AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0233340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0233340 MtrunA17_Chr2g0280881 99.153 118 1 0 1 118 1 118 2.47e-82 236
Msa0233340 MtrunA17_Chr2g0323211 64.151 106 38 0 9 114 5 110 8.65e-44 139
Msa0233340 MtrunA17_Chr2g0318861 59.649 114 42 1 1 114 1 110 1.71e-40 130
Msa0233340 MtrunA17_Chr8g0361421 59.091 110 45 0 5 114 2 111 2.75e-39 127
Msa0233340 MtrunA17_Chr2g0318791 61.905 105 33 2 14 118 9 106 2.51e-38 125
Msa0233340 MtrunA17_Chr4g0023231 59.406 101 41 0 14 114 6 106 2.52e-38 125
Msa0233340 MtrunA17_Chr2g0318761 65.957 94 31 1 14 107 5 97 4.30e-38 124
Msa0233340 MtrunA17_Chr2g0318801 63.830 94 33 1 14 107 9 101 7.75e-36 119
Msa0233340 MtrunA17_Chr8g0346481 56.436 101 44 0 14 114 19 119 1.21e-35 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0233340 AT4G27280.1 58.824 102 41 1 14 114 19 120 8.55e-38 124
Msa0233340 AT5G54490.1 55.882 102 44 1 14 114 19 120 1.15e-35 119
Msa0233340 AT2G46600.1 52.475 101 48 0 14 114 22 122 4.54e-34 115

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCAATGATGGAGAAAT+TGG 0.086263 2_2:+5677931 Msa0233340:CDS
AACCGATCCACCATTCTTCT+TGG 0.251298 2_2:-5677891 None:intergenic
GAGTGACTCTAAGCAACTTT+TGG 0.324838 2_2:+5678186 Msa0233340:CDS
CCCAATTTCTCCATCATTGA+AGG 0.334569 2_2:-5677932 None:intergenic
CCTTCAATGATGGAGAAATT+GGG 0.434103 2_2:+5677932 Msa0233340:CDS
AAACTCTGCATTGCTTGGGT+TGG 0.475429 2_2:+5678045 Msa0233340:CDS
CTAAGAGAAGGTGATATTGA+TGG 0.479725 2_2:+5678100 Msa0233340:CDS
GGGAAGTGAAGGGTTTATGA+AGG 0.496833 2_2:+5677952 Msa0233340:CDS
TCTGCATTGCTTGGGTTGGA+AGG 0.497566 2_2:+5678049 Msa0233340:CDS
GGACTACTTCCCTTCAATGA+TGG 0.499786 2_2:+5677922 Msa0233340:CDS
TTTATGAAGGAGTTGGCAAA+TGG 0.508232 2_2:+5677965 Msa0233340:CDS
TAAGAGAAGGTGATATTGAT+GGG 0.513310 2_2:+5678101 Msa0233340:CDS
GAAGGTGATATTGATGGGGA+TGG 0.523087 2_2:+5678106 Msa0233340:CDS
TGAAGGGTTTATGAAGGAGT+TGG 0.524979 2_2:+5677958 Msa0233340:CDS
CCAATTTCTCCATCATTGAA+GGG 0.539401 2_2:-5677931 None:intergenic
ATGGAGAAATTGGGAAGTGA+AGG 0.543176 2_2:+5677941 Msa0233340:CDS
GCGTCGAGTTCCAAGAAGAA+TGG 0.562292 2_2:+5677881 Msa0233340:CDS
GGATGGAGCTCTTAATGAGA+TGG 0.581359 2_2:+5678123 Msa0233340:CDS
TGGAGAAATTGGGAAGTGAA+GGG 0.582244 2_2:+5677942 Msa0233340:CDS
TTAGAGTCACTCATCAAAGC+AGG 0.587443 2_2:-5678175 None:intergenic
TTCCAAGAAGAATGGTGGAT+CGG 0.596507 2_2:+5677889 Msa0233340:CDS
CAGGACTCAACCTAAACATG+AGG 0.616612 2_2:-5678156 None:intergenic
ATTACTTGCATGCTAAGAGA+AGG 0.617118 2_2:+5678088 Msa0233340:CDS
TCGAGTTCCAAGAAGAATGG+TGG 0.624651 2_2:+5677884 Msa0233340:CDS
AAGAGAAGGTGATATTGATG+GGG 0.671155 2_2:+5678102 Msa0233340:CDS
AGGACTCAACCTAAACATGA+GGG 0.684724 2_2:-5678155 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTTTAATGTGTTGA+TGG + chr2_2:5677982-5678001 Msa0233340:CDS 25.0%
TAAGAGAAGGTGATATTGAT+GGG + chr2_2:5678101-5678120 Msa0233340:CDS 30.0%
CCAATTTCTCCATCATTGAA+GGG - chr2_2:5677934-5677953 None:intergenic 35.0%
! CCTTCAATGATGGAGAAATT+GGG + chr2_2:5677932-5677951 Msa0233340:CDS 35.0%
TTTATGAAGGAGTTGGCAAA+TGG + chr2_2:5677965-5677984 Msa0233340:CDS 35.0%
AAGAAAAACTCTGCATTGCT+TGG + chr2_2:5678040-5678059 Msa0233340:CDS 35.0%
AGAAAAACTCTGCATTGCTT+GGG + chr2_2:5678041-5678060 Msa0233340:CDS 35.0%
ATTACTTGCATGCTAAGAGA+AGG + chr2_2:5678088-5678107 Msa0233340:CDS 35.0%
CTAAGAGAAGGTGATATTGA+TGG + chr2_2:5678100-5678119 Msa0233340:CDS 35.0%
AAGAGAAGGTGATATTGATG+GGG + chr2_2:5678102-5678121 Msa0233340:CDS 35.0%
!! TTGGAAGAAGCTATTTTCAC+TGG + chr2_2:5678205-5678224 Msa0233340:CDS 35.0%
!! TTCCAAGAAGAATGGTGGAT+CGG + chr2_2:5677889-5677908 Msa0233340:CDS 40.0%
! CCCTTCAATGATGGAGAAAT+TGG + chr2_2:5677931-5677950 Msa0233340:CDS 40.0%
CCCAATTTCTCCATCATTGA+AGG - chr2_2:5677935-5677954 None:intergenic 40.0%
ATGGAGAAATTGGGAAGTGA+AGG + chr2_2:5677941-5677960 Msa0233340:CDS 40.0%
TGGAGAAATTGGGAAGTGAA+GGG + chr2_2:5677942-5677961 Msa0233340:CDS 40.0%
TGAAGGGTTTATGAAGGAGT+TGG + chr2_2:5677958-5677977 Msa0233340:CDS 40.0%
! AGTTTTGCACCCTCATGTTT+AGG + chr2_2:5678146-5678165 Msa0233340:CDS 40.0%
AGGACTCAACCTAAACATGA+GGG - chr2_2:5678158-5678177 None:intergenic 40.0%
TTAGAGTCACTCATCAAAGC+AGG - chr2_2:5678178-5678197 None:intergenic 40.0%
! GAGTGACTCTAAGCAACTTT+TGG + chr2_2:5678186-5678205 Msa0233340:CDS 40.0%
TCGAGTTCCAAGAAGAATGG+TGG + chr2_2:5677884-5677903 Msa0233340:CDS 45.0%
AACCGATCCACCATTCTTCT+TGG - chr2_2:5677894-5677913 None:intergenic 45.0%
!!! TGGTGGATCGGTTGTTTTTG+AGG + chr2_2:5677901-5677920 Msa0233340:CDS 45.0%
GGACTACTTCCCTTCAATGA+TGG + chr2_2:5677922-5677941 Msa0233340:CDS 45.0%
GGGAAGTGAAGGGTTTATGA+AGG + chr2_2:5677952-5677971 Msa0233340:CDS 45.0%
AAACTCTGCATTGCTTGGGT+TGG + chr2_2:5678045-5678064 Msa0233340:CDS 45.0%
GAAGGTGATATTGATGGGGA+TGG + chr2_2:5678106-5678125 Msa0233340:CDS 45.0%
GGATGGAGCTCTTAATGAGA+TGG + chr2_2:5678123-5678142 Msa0233340:CDS 45.0%
CAGGACTCAACCTAAACATG+AGG - chr2_2:5678159-5678178 None:intergenic 45.0%
GCGTCGAGTTCCAAGAAGAA+TGG + chr2_2:5677881-5677900 Msa0233340:CDS 50.0%
!! TCTGCATTGCTTGGGTTGGA+AGG + chr2_2:5678049-5678068 Msa0233340:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 5677878 5678234 5677878 ID=Msa0233340;Name=Msa0233340
chr2_2 mRNA 5677878 5678234 5677878 ID=Msa0233340-mRNA-1;Parent=Msa0233340;Name=Msa0233340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|118
chr2_2 exon 5677878 5678234 5677878 ID=Msa0233340-mRNA-1:exon:2428;Parent=Msa0233340-mRNA-1
chr2_2 CDS 5677878 5678234 5677878 ID=Msa0233340-mRNA-1:cds;Parent=Msa0233340-mRNA-1
Gene Sequence

>Msa0233340

ATGGCGTCGAGTTCCAAGAAGAATGGTGGATCGGTTGTTTTTGAGGACTACTTCCCTTCAATGATGGAGAAATTGGGAAGTGAAGGGTTTATGAAGGAGTTGGCAAATGGTTTTAATGTGTTGATGGACAGAGAGAAGAAAGTGATAACATTTGAGAGCTTGAAGAAAAACTCTGCATTGCTTGGGTTGGAAGGAATGAACGATGATGAGATTACTTGCATGCTAAGAGAAGGTGATATTGATGGGGATGGAGCTCTTAATGAGATGGAGTTTTGCACCCTCATGTTTAGGTTGAGTCCTGCTTTGATGAGTGACTCTAAGCAACTTTTGGAAGAAGCTATTTTCACTGGCCATTAA

Protein sequence

>Msa0233340

MASSSKKNGGSVVFEDYFPSMMEKLGSEGFMKELANGFNVLMDREKKVITFESLKKNSALLGLEGMNDDEITCMLREGDIDGDGALNEMEFCTLMFRLSPALMSDSKQLLEEAIFTGH*