AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0191040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191040 MtrunA17_Chr2g0280881 99.153 118 1 0 1 118 1 118 2.47e-82 236
Msa0191040 MtrunA17_Chr2g0323211 64.151 106 38 0 9 114 5 110 8.65e-44 139
Msa0191040 MtrunA17_Chr2g0318861 59.649 114 42 1 1 114 1 110 1.71e-40 130
Msa0191040 MtrunA17_Chr8g0361421 59.091 110 45 0 5 114 2 111 2.75e-39 127
Msa0191040 MtrunA17_Chr2g0318791 61.905 105 33 2 14 118 9 106 2.51e-38 125
Msa0191040 MtrunA17_Chr4g0023231 59.406 101 41 0 14 114 6 106 2.52e-38 125
Msa0191040 MtrunA17_Chr2g0318761 65.957 94 31 1 14 107 5 97 4.30e-38 124
Msa0191040 MtrunA17_Chr2g0318801 63.830 94 33 1 14 107 9 101 7.75e-36 119
Msa0191040 MtrunA17_Chr8g0346481 56.436 101 44 0 14 114 19 119 1.21e-35 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191040 AT4G27280.1 58.824 102 41 1 14 114 19 120 8.55e-38 124
Msa0191040 AT5G54490.1 55.882 102 44 1 14 114 19 120 1.15e-35 119
Msa0191040 AT2G46600.1 52.475 101 48 0 14 114 22 122 4.54e-34 115

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCAATGATGGAGAAAT+TGG 0.086263 2_1:+6567237 Msa0191040:CDS
AACCGATCCACCATTCTTCT+TGG 0.251298 2_1:-6567197 None:intergenic
GAGTGACTCTAAGCAACTTT+TGG 0.324838 2_1:+6567492 Msa0191040:CDS
CCCAATTTCTCCATCATTGA+AGG 0.334569 2_1:-6567238 None:intergenic
CCTTCAATGATGGAGAAATT+GGG 0.434103 2_1:+6567238 Msa0191040:CDS
AAACTCTGCATTGCTTGGGT+TGG 0.475429 2_1:+6567351 Msa0191040:CDS
CTAAGAGAAGGTGATATTGA+TGG 0.479725 2_1:+6567406 Msa0191040:CDS
GGGAAGTGAAGGGTTTATGA+AGG 0.496833 2_1:+6567258 Msa0191040:CDS
TCTGCATTGCTTGGGTTGGA+AGG 0.497566 2_1:+6567355 Msa0191040:CDS
GGACTACTTCCCTTCAATGA+TGG 0.499786 2_1:+6567228 Msa0191040:CDS
TTTATGAAGGAGTTGGCAAA+TGG 0.508232 2_1:+6567271 Msa0191040:CDS
TAAGAGAAGGTGATATTGAT+GGG 0.513310 2_1:+6567407 Msa0191040:CDS
GAAGGTGATATTGATGGGGA+TGG 0.523087 2_1:+6567412 Msa0191040:CDS
TGAAGGGTTTATGAAGGAGT+TGG 0.524979 2_1:+6567264 Msa0191040:CDS
CCAATTTCTCCATCATTGAA+GGG 0.539401 2_1:-6567237 None:intergenic
ATGGAGAAATTGGGAAGTGA+AGG 0.543176 2_1:+6567247 Msa0191040:CDS
GCGTCGAGTTCCAAGAAGAA+TGG 0.562292 2_1:+6567187 Msa0191040:CDS
GGATGGAGCTCTTAATGAGA+TGG 0.581359 2_1:+6567429 Msa0191040:CDS
TGGAGAAATTGGGAAGTGAA+GGG 0.582244 2_1:+6567248 Msa0191040:CDS
TTAGAGTCACTCATCAAAGC+AGG 0.587443 2_1:-6567481 None:intergenic
TTCCAAGAAGAATGGTGGAT+CGG 0.596507 2_1:+6567195 Msa0191040:CDS
CAGGACTCAACCTAAACATG+AGG 0.616612 2_1:-6567462 None:intergenic
ATTACTTGCATGCTAAGAGA+AGG 0.617118 2_1:+6567394 Msa0191040:CDS
TCGAGTTCCAAGAAGAATGG+TGG 0.624651 2_1:+6567190 Msa0191040:CDS
AAGAGAAGGTGATATTGATG+GGG 0.671155 2_1:+6567408 Msa0191040:CDS
AGGACTCAACCTAAACATGA+GGG 0.684724 2_1:-6567461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTTTAATGTGTTGA+TGG + chr2_1:6567288-6567307 Msa0191040:CDS 25.0%
TAAGAGAAGGTGATATTGAT+GGG + chr2_1:6567407-6567426 Msa0191040:CDS 30.0%
CCAATTTCTCCATCATTGAA+GGG - chr2_1:6567240-6567259 None:intergenic 35.0%
! CCTTCAATGATGGAGAAATT+GGG + chr2_1:6567238-6567257 Msa0191040:CDS 35.0%
TTTATGAAGGAGTTGGCAAA+TGG + chr2_1:6567271-6567290 Msa0191040:CDS 35.0%
AAGAAAAACTCTGCATTGCT+TGG + chr2_1:6567346-6567365 Msa0191040:CDS 35.0%
AGAAAAACTCTGCATTGCTT+GGG + chr2_1:6567347-6567366 Msa0191040:CDS 35.0%
ATTACTTGCATGCTAAGAGA+AGG + chr2_1:6567394-6567413 Msa0191040:CDS 35.0%
CTAAGAGAAGGTGATATTGA+TGG + chr2_1:6567406-6567425 Msa0191040:CDS 35.0%
AAGAGAAGGTGATATTGATG+GGG + chr2_1:6567408-6567427 Msa0191040:CDS 35.0%
!! TTGGAAGAAGCTATTTTCAC+TGG + chr2_1:6567511-6567530 Msa0191040:CDS 35.0%
!! TTCCAAGAAGAATGGTGGAT+CGG + chr2_1:6567195-6567214 Msa0191040:CDS 40.0%
! CCCTTCAATGATGGAGAAAT+TGG + chr2_1:6567237-6567256 Msa0191040:CDS 40.0%
CCCAATTTCTCCATCATTGA+AGG - chr2_1:6567241-6567260 None:intergenic 40.0%
ATGGAGAAATTGGGAAGTGA+AGG + chr2_1:6567247-6567266 Msa0191040:CDS 40.0%
TGGAGAAATTGGGAAGTGAA+GGG + chr2_1:6567248-6567267 Msa0191040:CDS 40.0%
TGAAGGGTTTATGAAGGAGT+TGG + chr2_1:6567264-6567283 Msa0191040:CDS 40.0%
! AGTTTTGCACCCTCATGTTT+AGG + chr2_1:6567452-6567471 Msa0191040:CDS 40.0%
AGGACTCAACCTAAACATGA+GGG - chr2_1:6567464-6567483 None:intergenic 40.0%
TTAGAGTCACTCATCAAAGC+AGG - chr2_1:6567484-6567503 None:intergenic 40.0%
! GAGTGACTCTAAGCAACTTT+TGG + chr2_1:6567492-6567511 Msa0191040:CDS 40.0%
TCGAGTTCCAAGAAGAATGG+TGG + chr2_1:6567190-6567209 Msa0191040:CDS 45.0%
AACCGATCCACCATTCTTCT+TGG - chr2_1:6567200-6567219 None:intergenic 45.0%
!!! TGGTGGATCGGTTGTTTTTG+AGG + chr2_1:6567207-6567226 Msa0191040:CDS 45.0%
GGACTACTTCCCTTCAATGA+TGG + chr2_1:6567228-6567247 Msa0191040:CDS 45.0%
GGGAAGTGAAGGGTTTATGA+AGG + chr2_1:6567258-6567277 Msa0191040:CDS 45.0%
AAACTCTGCATTGCTTGGGT+TGG + chr2_1:6567351-6567370 Msa0191040:CDS 45.0%
GAAGGTGATATTGATGGGGA+TGG + chr2_1:6567412-6567431 Msa0191040:CDS 45.0%
GGATGGAGCTCTTAATGAGA+TGG + chr2_1:6567429-6567448 Msa0191040:CDS 45.0%
CAGGACTCAACCTAAACATG+AGG - chr2_1:6567465-6567484 None:intergenic 45.0%
GCGTCGAGTTCCAAGAAGAA+TGG + chr2_1:6567187-6567206 Msa0191040:CDS 50.0%
!! TCTGCATTGCTTGGGTTGGA+AGG + chr2_1:6567355-6567374 Msa0191040:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 6567184 6567540 6567184 ID=Msa0191040;Name=Msa0191040
chr2_1 mRNA 6567184 6567540 6567184 ID=Msa0191040-mRNA-1;Parent=Msa0191040;Name=Msa0191040-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|118
chr2_1 exon 6567184 6567540 6567184 ID=Msa0191040-mRNA-1:exon:2937;Parent=Msa0191040-mRNA-1
chr2_1 CDS 6567184 6567540 6567184 ID=Msa0191040-mRNA-1:cds;Parent=Msa0191040-mRNA-1
Gene Sequence

>Msa0191040

ATGGCGTCGAGTTCCAAGAAGAATGGTGGATCGGTTGTTTTTGAGGACTACTTCCCTTCAATGATGGAGAAATTGGGAAGTGAAGGGTTTATGAAGGAGTTGGCAAATGGTTTTAATGTGTTGATGGACAGAGAGAAGAAAGTGATAACATTTGAGAGCTTGAAGAAAAACTCTGCATTGCTTGGGTTGGAAGGAATGAACGATGATGAGATTACTTGCATGCTAAGAGAAGGTGATATTGATGGGGATGGAGCTCTTAATGAGATGGAGTTTTGCACCCTCATGTTTAGGTTGAGTCCTGCTTTGATGAGTGACTCTAAGCAACTTTTGGAAGAAGCTATTTTCACTGGCCATTAA

Protein sequence

>Msa0191040

MASSSKKNGGSVVFEDYFPSMMEKLGSEGFMKELANGFNVLMDREKKVITFESLKKNSALLGLEGMNDDEITCMLREGDIDGDGALNEMEFCTLMFRLSPALMSDSKQLLEEAIFTGH*