AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0006920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006920 MtrunA17_Chr1g0148961 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0006920 MtrunA17_Chr1g0148951 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0006920 MtrunA17_Chr1g0184901 86.567 134 18 0 1 134 1 134 1.96e-73 215
Msa0006920 MtrunA17_Chr1g0211651 81.250 128 24 0 7 134 4 131 2.88e-71 209
Msa0006920 MtrunA17_Chr4g0024701 66.418 134 25 1 1 134 1 114 6.90e-47 147
Msa0006920 MtrunA17_Chr2g0319191 64.545 110 37 2 24 132 14 122 1.42e-40 132
Msa0006920 MtrunA17_Chr2g0319181 64.545 110 37 2 24 132 14 122 1.91e-40 131
Msa0006920 MtrunA17_Chr7g0268831 58.333 132 47 4 1 130 1 126 2.47e-40 131
Msa0006920 MtrunA17_Chr7g0273351 58.197 122 49 2 10 130 3 123 8.09e-40 130
Msa0006920 MtrunA17_Chr7g0273331 58.197 122 49 2 10 130 59 179 9.14e-39 129
Msa0006920 MtrunA17_Chr7g0259151 61.682 107 39 2 25 130 21 126 2.97e-38 126
Msa0006920 MtrunA17_Chr7g0272611 55.652 115 49 2 19 132 19 132 7.29e-37 123
Msa0006920 MtrunA17_Chr4g0031041 59.292 113 44 2 23 134 15 126 4.29e-36 121
Msa0006920 MtrunA17_Chr2g0327951 59.292 113 44 2 23 134 56 167 8.62e-36 121
Msa0006920 MtrunA17_Chr4g0035621 61.111 108 40 2 27 133 25 131 4.35e-35 118
Msa0006920 MtrunA17_Chr8g0379311 60.550 109 41 2 27 134 25 132 4.84e-35 118
Msa0006920 MtrunA17_Chr4g0032301 59.633 109 42 2 27 134 25 132 5.44e-35 118
Msa0006920 MtrunA17_Chr4g0031001 59.633 109 42 2 27 134 25 132 6.61e-35 118
Msa0006920 MtrunA17_Chr4g0031651 59.633 109 42 2 27 134 25 132 6.76e-35 118
Msa0006920 MtrunA17_Chr2g0327931 60.185 108 41 2 27 133 24 130 8.79e-35 118
Msa0006920 MtrunA17_Chr7g0273341 53.448 116 40 5 19 132 50 153 3.35e-28 101
Msa0006920 MtrunA17_Chr4g0031031 62.687 67 24 1 55 120 17 83 3.79e-20 79.3
Msa0006920 MtrunA17_Chr6g0449661 45.283 106 47 4 27 130 86 182 6.63e-19 78.6
Msa0006920 MtrunA17_Chr3g0119971 44.340 106 48 4 27 130 81 177 1.70e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006920 AT1G52740.1 88.060 134 16 0 1 134 1 134 5.27e-76 222
Msa0006920 AT3G54560.1 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0006920 AT3G54560.2 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0006920 AT2G38810.1 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0006920 AT2G38810.4 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0006920 AT2G38810.3 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0006920 AT2G38810.2 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0006920 AT4G13570.1 66.667 111 33 1 18 124 8 118 3.28e-45 143
Msa0006920 AT4G13570.2 75.556 90 22 0 35 124 35 124 3.59e-44 141
Msa0006920 AT4G27230.2 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0006920 AT4G27230.1 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0006920 AT5G54640.1 64.545 110 37 2 24 132 14 122 2.45e-40 131
Msa0006920 AT3G20670.1 65.741 108 35 2 24 130 14 120 6.89e-40 130
Msa0006920 AT1G08880.1 64.762 105 35 2 25 128 21 124 1.01e-39 130
Msa0006920 AT1G51060.1 63.636 110 38 2 24 132 14 122 1.11e-39 130
Msa0006920 AT1G54690.1 63.810 105 36 2 25 128 21 124 2.89e-39 129
Msa0006920 AT5G02560.1 61.947 113 41 2 23 134 21 132 1.19e-38 128
Msa0006920 AT5G27670.1 60.870 115 43 2 19 132 18 131 4.54e-37 124
Msa0006920 AT5G59870.1 61.261 111 41 2 23 132 21 130 3.64e-36 121
Msa0006920 AT5G02560.2 51.095 137 41 3 23 134 21 156 3.79e-33 115

Find 54 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGTCTTTGAGGTTTGC+TGG 0.259581 1_1:-9924996 None:intergenic
TCTTCTTGTCCTTGTCTTTG+AGG 0.316058 1_1:-9925004 None:intergenic
TTCCTCAAGAGCTGGTCTTC+AGG 0.343744 1_1:+9925040 Msa0006920:CDS
TATGAACATTCATTCCTTTG+AGG 0.376752 1_1:-9927873 None:intergenic
GGTGATAACAACTTTATTTG+TGG 0.407928 1_1:+9927943 Msa0006920:three_prime_UTR
CAATGGCACATGGAAGAGTT+GGG 0.442531 1_1:+9927630 Msa0006920:CDS
AGTTTATTCTGCCGCTATCC+TGG 0.451988 1_1:+9927664 Msa0006920:CDS
GTATTTAACTGCAGAGGTGT+TGG 0.470183 1_1:+9927688 Msa0006920:CDS
ACAATGGCACATGGAAGAGT+TGG 0.476378 1_1:+9927629 Msa0006920:CDS
GGAGCGAAGGGTTTGACAAC+CGG 0.477894 1_1:+9924966 Msa0006920:CDS
CTCATCAAAGGAACAATAGC+TGG 0.487626 1_1:+9927806 Msa0006920:CDS
TTGAAGTGCGAAAACATGTC+GGG 0.497395 1_1:+9924939 Msa0006920:exon
TGCATGTCTAGGATTTATGT+TGG 0.504995 1_1:+9928086 Msa0006920:three_prime_UTR
GGATAATTCGCTCCTTGCGT+TGG 0.505084 1_1:+9927964 Msa0006920:three_prime_UTR
ATAAGAGACTTGTGGATATG+AGG 0.507095 1_1:-9927842 None:intergenic
TGGTATGGTACTGCATGTCT+AGG 0.516636 1_1:+9928075 Msa0006920:three_prime_UTR
AGACGATGGATACGTCCGAC+AGG 0.539450 1_1:-9927593 None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG 0.540859 1_1:+9927694 Msa0006920:CDS
AGCTCTTGAGGAACGAGATG+TGG 0.542232 1_1:-9925030 None:intergenic
AACCTGAAGACCAGCTCTTG+AGG 0.543571 1_1:-9925042 None:intergenic
ACATCTCGTTCCTCAAGAGC+TGG 0.546311 1_1:+9925032 Msa0006920:CDS
TAATTCTCTCTAATTAACGC+AGG 0.546344 1_1:+9927894 Msa0006920:three_prime_UTR
GCAGAGGTGTTGGAGTTGGC+TGG 0.558822 1_1:+9927698 Msa0006920:CDS
CAATGCAAGCAAGGATCTGA+AGG 0.565081 1_1:+9927721 Msa0006920:CDS
TGCGAAAACATGTCGGGGAA+AGG 0.567757 1_1:+9924945 Msa0006920:exon
CCTCTGCAGTTAAATACTCC+AGG 0.571308 1_1:-9927682 None:intergenic
GTTGGCTGGCAATGCAAGCA+AGG 0.571379 1_1:+9927712 Msa0006920:CDS
GAACTTGACACTCTCATCAA+AGG 0.571379 1_1:+9927794 Msa0006920:CDS
AAATTCAGATAATCTAAAGA+AGG 0.572497 1_1:-9928005 None:intergenic
ACTTGTTGATAAGAGACTTG+TGG 0.572936 1_1:-9927850 None:intergenic
TCAGATAATCTAAAGAAGGA+TGG 0.582849 1_1:-9928001 None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG 0.584334 1_1:-9927758 None:intergenic
TCTTATCAACAAGTCCTCAA+AGG 0.584732 1_1:+9927859 Msa0006920:CDS
CATCTACAGCTTGCTATCCG+TGG 0.586408 1_1:+9927764 Msa0006920:CDS
TCCATCGTCTTCTGAAGTCA+AGG 0.589423 1_1:+9927606 Msa0006920:CDS
TTTGAAGTGCGAAAACATGT+CGG 0.591882 1_1:+9924938 Msa0006920:exon
CATGTCGGGGAAAGGAGCGA+AGG 0.599837 1_1:+9924953 Msa0006920:exon
AATTCTCTCTAATTAACGCA+GGG 0.600377 1_1:+9927895 Msa0006920:three_prime_UTR
GCTCTTGAGGAACGAGATGT+GGG 0.607406 1_1:-9925029 None:intergenic
AAAGGAACAATAGCTGGTGG+TGG 0.608922 1_1:+9927812 Msa0006920:CDS
AAGTCAAGGACAATGGCACA+TGG 0.622887 1_1:+9927620 Msa0006920:CDS
ATGTCGGGGAAAGGAGCGAA+GGG 0.624226 1_1:+9924954 Msa0006920:CDS
CCTGGAGTATTTAACTGCAG+AGG 0.624849 1_1:+9927682 Msa0006920:CDS
CAATCAATCAAACCAACGCA+AGG 0.626065 1_1:-9927976 None:intergenic
GACGATGGATACGTCCGACA+GGG 0.626692 1_1:-9927592 None:intergenic
TTCTACATACAGTTCCCTGT+CGG 0.628096 1_1:+9927578 Msa0006920:intron
TGTCAAGTTCTTCATCTCCA+CGG 0.636093 1_1:-9927781 None:intergenic
ACCAGCAAACCTCAAAGACA+AGG 0.637885 1_1:+9924995 Msa0006920:CDS
AATGGCACATGGAAGAGTTG+GGG 0.645846 1_1:+9927631 Msa0006920:CDS
TGAAGTGCGAAAACATGTCG+GGG 0.649052 1_1:+9924940 Msa0006920:exon
TCTTCTGAAGTCAAGGACAA+TGG 0.655563 1_1:+9927613 Msa0006920:CDS
ATCAAAGGAACAATAGCTGG+TGG 0.661257 1_1:+9927809 Msa0006920:CDS
TCCTTGACTTCAGAAGACGA+TGG 0.668973 1_1:-9927607 None:intergenic
AGTTAAATACTCCAGGATAG+CGG 0.673071 1_1:-9927675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTATTTTTTTGTTAAA+AGG + chr1_1:9925234-9925253 Msa0006920:intron 10.0%
!! TTTATGATTTATAATATCAA+TGG + chr1_1:9925854-9925873 Msa0006920:intron 10.0%
!!! TTTTTTTGTTAAAAGGATAA+TGG + chr1_1:9925241-9925260 Msa0006920:intron 15.0%
!!! TTTTTTGTTAAAAGGATAAT+GGG + chr1_1:9925242-9925261 Msa0006920:intron 15.0%
!! ACAAAAAGAATAGTAAAAAA+CGG - chr1_1:9925521-9925540 None:intergenic 15.0%
!!! TTTTTTTGTTTGTTTTAAGT+AGG + chr1_1:9926583-9926602 Msa0006920:intron 15.0%
!!! AGTATTATTTTATAACTGTT+TGG + chr1_1:9927025-9927044 Msa0006920:intron 15.0%
!!! AAAGGATTTTAATGTAATTT+CGG + chr1_1:9927922-9927941 Msa0006920:three_prime_UTR 15.0%
!!! AAAATTGTAGTTTGAGATTT+GGG + chr1_1:9925283-9925302 Msa0006920:intron 20.0%
!!! TTTTTATCTAAGATGAAGTA+TGG + chr1_1:9925306-9925325 Msa0006920:intron 20.0%
!!! TTTTATCTAAGATGAAGTAT+GGG + chr1_1:9925307-9925326 Msa0006920:intron 20.0%
!!! TTTTTACCAAGATTGAAATT+TGG + chr1_1:9925347-9925366 Msa0006920:intron 20.0%
!!! TTTTACCAAGATTGAAATTT+GGG + chr1_1:9925348-9925367 Msa0006920:intron 20.0%
!! ATATATACACCTATTAAGAA+GGG - chr1_1:9926139-9926158 None:intergenic 20.0%
!! AATATATACACCTATTAAGA+AGG - chr1_1:9926140-9926159 None:intergenic 20.0%
!! ATATATTCAGTAATGCTAAA+TGG + chr1_1:9926153-9926172 Msa0006920:intron 20.0%
!! ATTATAAAAGATAGTCCATT+CGG - chr1_1:9926210-9926229 None:intergenic 20.0%
!! TGGAAAATGAAAAGATAATT+TGG - chr1_1:9926290-9926309 None:intergenic 20.0%
!!! CTTCGATATTGATTAATTAA+TGG - chr1_1:9926471-9926490 None:intergenic 20.0%
!! TTAATTAATCAATATCGAAG+TGG + chr1_1:9926471-9926490 Msa0006920:intron 20.0%
!! TTATAGAATCATATGTGAAT+GGG + chr1_1:9926608-9926627 Msa0006920:intron 20.0%
!! TATTGTATTTCTTTGATTCT+TGG + chr1_1:9927436-9927455 Msa0006920:intron 20.0%
!! AAATTCAGATAATCTAAAGA+AGG - chr1_1:9928008-9928027 None:intergenic 20.0%
!!! ATTTTTCTTCTCTTTTGATA+TGG + chr1_1:9928024-9928043 Msa0006920:three_prime_UTR 20.0%
! AGTGAAAAAGGTTAAATTTG+AGG - chr1_1:9925130-9925149 None:intergenic 25.0%
!! GAAAATTGTAGTTTGAGATT+TGG + chr1_1:9925282-9925301 Msa0006920:intron 25.0%
!! TTTATCTAAGATGAAGTATG+GGG + chr1_1:9925308-9925327 Msa0006920:intron 25.0%
! CAGTGAAAAACTAATAACAT+GGG - chr1_1:9925428-9925447 None:intergenic 25.0%
! TCAGTGAAAAACTAATAACA+TGG - chr1_1:9925429-9925448 None:intergenic 25.0%
! CAGTTAAGCAAATTGTAAAT+GGG - chr1_1:9925452-9925471 None:intergenic 25.0%
! TCAGTTAAGCAAATTGTAAA+TGG - chr1_1:9925453-9925472 None:intergenic 25.0%
! AAAAGAATAGTAAAAAACGG+TGG - chr1_1:9925518-9925537 None:intergenic 25.0%
!!! AATTACCTTGTTTTGTTTCA+TGG + chr1_1:9925550-9925569 Msa0006920:intron 25.0%
! GAGAATTGAATAGTTGAATA+TGG + chr1_1:9925623-9925642 Msa0006920:intron 25.0%
!!! GGATGTTCATTTTATTTCTT+TGG + chr1_1:9925756-9925775 Msa0006920:intron 25.0%
! CAAAAGAAGAAATCAAATCA+TGG - chr1_1:9925801-9925820 None:intergenic 25.0%
!! GCAATTCATTTTACAACTTA+AGG - chr1_1:9925893-9925912 None:intergenic 25.0%
!!! AAATGAATTGCTTACTTTTG+AGG + chr1_1:9925902-9925921 Msa0006920:intron 25.0%
! AGAGAACATATGCAAAATAA+GGG - chr1_1:9925936-9925955 None:intergenic 25.0%
!!! TTTTCTTTTTTCGGGAAAAA+AGG - chr1_1:9926047-9926066 None:intergenic 25.0%
! CTATTATTTCCCTTCTTAAT+AGG + chr1_1:9926127-9926146 Msa0006920:intron 25.0%
!!! AAAAGATAATTTGGTGTAAG+GGG - chr1_1:9926281-9926300 None:intergenic 25.0%
!!! GAAAAGATAATTTGGTGTAA+GGG - chr1_1:9926282-9926301 None:intergenic 25.0%
!!! TGAAAAGATAATTTGGTGTA+AGG - chr1_1:9926283-9926302 None:intergenic 25.0%
! CAAAAAAACAATCAATCCAT+TGG - chr1_1:9926571-9926590 None:intergenic 25.0%
! GTTATAGAATCATATGTGAA+TGG + chr1_1:9926607-9926626 Msa0006920:intron 25.0%
! TATAGAATCATATGTGAATG+GGG + chr1_1:9926609-9926628 Msa0006920:intron 25.0%
!! AGTTATCACATAATCTTTTG+TGG + chr1_1:9926819-9926838 Msa0006920:intron 25.0%
!! ACAGATTTTCACATATACAT+GGG - chr1_1:9927106-9927125 None:intergenic 25.0%
!! AACAGATTTTCACATATACA+TGG - chr1_1:9927107-9927126 None:intergenic 25.0%
! AAAAAGACAAAATCATCACA+CGG - chr1_1:9927387-9927406 None:intergenic 25.0%
AAGAAAACGATGAAGAGAAA+GGG - chr1_1:9925087-9925106 None:intergenic 30.0%
CCAAAACAAAAGAGTGAAAA+AGG - chr1_1:9925142-9925161 None:intergenic 30.0%
!! CCTTTTTCACTCTTTTGTTT+TGG + chr1_1:9925139-9925158 Msa0006920:intron 30.0%
!!! ATGGGTTTTACTTTTTGTAG+AGG + chr1_1:9925260-9925279 Msa0006920:intron 30.0%
GATTTATTGTGTCACATTGA+TGG + chr1_1:9925471-9925490 Msa0006920:intron 30.0%
CTTTAGATTAAAGACGTCTT+CGG - chr1_1:9925715-9925734 None:intergenic 30.0%
! ACGTCTTTAATCTAAAGTGT+TGG + chr1_1:9925718-9925737 Msa0006920:intron 30.0%
!! TGTTGGTGCTACATTATTTA+TGG + chr1_1:9925735-9925754 Msa0006920:intron 30.0%
CAGAGAACATATGCAAAATA+AGG - chr1_1:9925937-9925956 None:intergenic 30.0%
! TATTTTGCATATGTTCTCTG+TGG + chr1_1:9925937-9925956 Msa0006920:intron 30.0%
TTGTTTGAAAAGCTTAGACA+AGG - chr1_1:9925970-9925989 None:intergenic 30.0%
! ACTTGCGCATTTTCTTTTTT+CGG - chr1_1:9926056-9926075 None:intergenic 30.0%
TGGATATTAGAAGGGATATA+GGG + chr1_1:9926084-9926103 Msa0006920:intron 30.0%
AAGAAGGGAAATAATAGTTC+CGG - chr1_1:9926124-9926143 None:intergenic 30.0%
GATTTCAATCCAATGATCAT+TGG - chr1_1:9926441-9926460 None:intergenic 30.0%
TTAATCAATATCGAAGTGGT+TGG + chr1_1:9926475-9926494 Msa0006920:intron 30.0%
TAATCAATATCGAAGTGGTT+GGG + chr1_1:9926476-9926495 Msa0006920:intron 30.0%
TTGCAGTCAAGATTAGTAAT+TGG + chr1_1:9926529-9926548 Msa0006920:intron 30.0%
! ATGGATTACATAAAACACAG+AGG - chr1_1:9927210-9927229 None:intergenic 30.0%
TTAGTAATCCAAAAACGAGA+AGG - chr1_1:9927236-9927255 None:intergenic 30.0%
!! TCGTTTTTGGATTACTAATG+TGG + chr1_1:9927238-9927257 Msa0006920:intron 30.0%
GTAAATCATGTTGCATTTGT+TGG + chr1_1:9927503-9927522 Msa0006920:intron 30.0%
ATTTATCCCACATCATAATC+TGG + chr1_1:9927536-9927555 Msa0006920:intron 30.0%
ATGTAGAAAGATGTTCATGA+TGG - chr1_1:9927566-9927585 None:intergenic 30.0%
TATGAACATTCATTCCTTTG+AGG - chr1_1:9927876-9927895 None:intergenic 30.0%
TAATTCTCTCTAATTAACGC+AGG + chr1_1:9927894-9927913 Msa0006920:three_prime_UTR 30.0%
AATTCTCTCTAATTAACGCA+GGG + chr1_1:9927895-9927914 Msa0006920:three_prime_UTR 30.0%
TAATTAACGCAGGGAAAAAA+AGG + chr1_1:9927904-9927923 Msa0006920:three_prime_UTR 30.0%
! GGTGATAACAACTTTATTTG+TGG + chr1_1:9927943-9927962 Msa0006920:three_prime_UTR 30.0%
TCAGATAATCTAAAGAAGGA+TGG - chr1_1:9928004-9928023 None:intergenic 30.0%
!! AACTAACATCTTCACTTTTG+TGG + chr1_1:9928055-9928074 Msa0006920:three_prime_UTR 30.0%
!! TCTATCTGTTTTGAATTGCT+TGG + chr1_1:9928129-9928148 Msa0006920:three_prime_UTR 30.0%
! TTTGAAGTGCGAAAACATGT+CGG + chr1_1:9924938-9924957 Msa0006920:exon 35.0%
CAAGAAAACGATGAAGAGAA+AGG - chr1_1:9925088-9925107 None:intergenic 35.0%
CACTTTAGCGATGTAACATA+AGG - chr1_1:9925188-9925207 None:intergenic 35.0%
GTACACCCAAATTTCAATCT+TGG - chr1_1:9925356-9925375 None:intergenic 35.0%
ATTCGCCATGAAACAAAACA+AGG - chr1_1:9925558-9925577 None:intergenic 35.0%
TTGAATATGGTCACATGTGT+CGG + chr1_1:9925636-9925655 Msa0006920:intron 35.0%
! CTTGCGCATTTTCTTTTTTC+GGG - chr1_1:9926055-9926074 None:intergenic 35.0%
GAAAAAAGAAAATGCGCAAG+TGG + chr1_1:9926055-9926074 Msa0006920:intron 35.0%
GTGGATATTAGAAGGGATAT+AGG + chr1_1:9926083-9926102 Msa0006920:intron 35.0%
ATATTAGAAGGGATATAGGG+TGG + chr1_1:9926087-9926106 Msa0006920:intron 35.0%
AGAAGGGAAATAATAGTTCC+GGG - chr1_1:9926123-9926142 None:intergenic 35.0%
TAAAGCTTGTACAGTGTAAG+CGG - chr1_1:9926385-9926404 None:intergenic 35.0%
CTGTCATAACCAATGATCAT+TGG + chr1_1:9926429-9926448 Msa0006920:intron 35.0%
!!! TGTTTTAACTGTCAAGACCA+AGG - chr1_1:9926957-9926976 None:intergenic 35.0%
ATGCTGTGTTCAATTAGACA+TGG - chr1_1:9927148-9927167 None:intergenic 35.0%
TCTGCATTACTTATTCTGTG+CGG + chr1_1:9927174-9927193 Msa0006920:intron 35.0%
! TCCATATTCCTTCTCGTTTT+TGG + chr1_1:9927225-9927244 Msa0006920:intron 35.0%
TCCAAAAACGAGAAGGAATA+TGG - chr1_1:9927229-9927248 None:intergenic 35.0%
CCACTTGCAAAAAATTCTAG+TGG - chr1_1:9927411-9927430 None:intergenic 35.0%
!!! CCACTAGAATTTTTTGCAAG+TGG + chr1_1:9927408-9927427 Msa0006920:intron 35.0%
AAGCATGCTAAAATGTTGTC+TGG + chr1_1:9927478-9927497 Msa0006920:intron 35.0%
GGAAAACCAGATTATGATGT+GGG - chr1_1:9927545-9927564 None:intergenic 35.0%
TGGAAAACCAGATTATGATG+TGG - chr1_1:9927546-9927565 None:intergenic 35.0%
AGTTAAATACTCCAGGATAG+CGG - chr1_1:9927678-9927697 None:intergenic 35.0%
ATAAGAGACTTGTGGATATG+AGG - chr1_1:9927845-9927864 None:intergenic 35.0%
! ACTTGTTGATAAGAGACTTG+TGG - chr1_1:9927853-9927872 None:intergenic 35.0%
TCTTATCAACAAGTCCTCAA+AGG + chr1_1:9927859-9927878 Msa0006920:CDS 35.0%
!! ACATCTTCACTTTTGTGGTA+TGG + chr1_1:9928060-9928079 Msa0006920:three_prime_UTR 35.0%
! TGCATGTCTAGGATTTATGT+TGG + chr1_1:9928086-9928105 Msa0006920:three_prime_UTR 35.0%
!! TGTTTTGAATTGCTTGGCAA+TGG + chr1_1:9928135-9928154 Msa0006920:three_prime_UTR 35.0%
!!! AGAGAGAGATTTGGCTTTTG+AGG - chr1_1:9924895-9924914 None:intergenic 40.0%
TTGAAGTGCGAAAACATGTC+GGG + chr1_1:9924939-9924958 Msa0006920:exon 40.0%
TCTTCTTGTCCTTGTCTTTG+AGG - chr1_1:9925007-9925026 None:intergenic 40.0%
GATGAAGAGAAAGGGTAAGA+GGG - chr1_1:9925079-9925098 None:intergenic 40.0%
AGATGAAGTATGGGGTAGAA+AGG + chr1_1:9925316-9925335 Msa0006920:intron 40.0%
!! TGTCACATTGATGGTGTCTT+TGG + chr1_1:9925480-9925499 Msa0006920:intron 40.0%
!! ACATTGATGGTGTCTTTGGA+AGG + chr1_1:9925484-9925503 Msa0006920:intron 40.0%
!! CATTGATGGTGTCTTTGGAA+GGG + chr1_1:9925485-9925504 Msa0006920:intron 40.0%
!! ATTGATGGTGTCTTTGGAAG+GGG + chr1_1:9925486-9925505 Msa0006920:intron 40.0%
TTGGACTTCTAACAGAGACT+AGG + chr1_1:9925775-9925794 Msa0006920:intron 40.0%
CATATGTTCTCTGTGGTCAT+GGG + chr1_1:9925944-9925963 Msa0006920:intron 40.0%
ATCCCTTCTAATATCCACCA+AGG - chr1_1:9926081-9926100 None:intergenic 40.0%
TATTAGCTAACTATGCGGTG+TGG - chr1_1:9926310-9926329 None:intergenic 40.0%
ACACCGCATAGTTAGCTAAT+AGG + chr1_1:9926309-9926328 Msa0006920:intron 40.0%
CTGCCTATTAGCTAACTATG+CGG - chr1_1:9926315-9926334 None:intergenic 40.0%
ATGACAGTGTGTGCTATCAA+GGG - chr1_1:9926416-9926435 None:intergenic 40.0%
TATGACAGTGTGTGCTATCA+AGG - chr1_1:9926417-9926436 None:intergenic 40.0%
CTTGACTGCAACGTAAAAGA+TGG - chr1_1:9926520-9926539 None:intergenic 40.0%
ATGCTTCACTCTCAAACCAA+TGG + chr1_1:9926552-9926571 Msa0006920:intron 40.0%
AGAATCATATGTGAATGGGG+TGG + chr1_1:9926612-9926631 Msa0006920:intron 40.0%
! CATAATCTTTTGTGGTCCAG+TGG + chr1_1:9926827-9926846 Msa0006920:intron 40.0%
CCAAAAAAGCAGATTACCAC+TGG - chr1_1:9926846-9926865 None:intergenic 40.0%
!!! CCAGTGGTAATCTGCTTTTT+TGG + chr1_1:9926843-9926862 Msa0006920:intron 40.0%
!!! CTGCTTTTTTGGTTCCTATG+AGG + chr1_1:9926854-9926873 Msa0006920:intron 40.0%
!! AGCTTGGATGGTTTTTCAGA+GGG - chr1_1:9927053-9927072 None:intergenic 40.0%
TGCTTCATCCACATGTGTTA+CGG + chr1_1:9927329-9927348 Msa0006920:intron 40.0%
CCAAAGATAAGTCACTCACA+TGG - chr1_1:9927361-9927380 None:intergenic 40.0%
CCATGTGAGTGACTTATCTT+TGG + chr1_1:9927358-9927377 Msa0006920:intron 40.0%
TTCTACATACAGTTCCCTGT+CGG + chr1_1:9927578-9927597 Msa0006920:intron 40.0%
!! TCTTCTGAAGTCAAGGACAA+TGG + chr1_1:9927613-9927632 Msa0006920:CDS 40.0%
! GTATTTAACTGCAGAGGTGT+TGG + chr1_1:9927688-9927707 Msa0006920:CDS 40.0%
ATAGCAAGCTGTAGATGTCT+CGG - chr1_1:9927761-9927780 None:intergenic 40.0%
TGTCAAGTTCTTCATCTCCA+CGG - chr1_1:9927784-9927803 None:intergenic 40.0%
GAACTTGACACTCTCATCAA+AGG + chr1_1:9927794-9927813 Msa0006920:CDS 40.0%
CTCATCAAAGGAACAATAGC+TGG + chr1_1:9927806-9927825 Msa0006920:CDS 40.0%
ATCAAAGGAACAATAGCTGG+TGG + chr1_1:9927809-9927828 Msa0006920:CDS 40.0%
CAATCAATCAAACCAACGCA+AGG - chr1_1:9927979-9927998 None:intergenic 40.0%
! AATGCAAAGTCTTTTGCTGC+TGG + chr1_1:9928161-9928180 Msa0006920:three_prime_UTR 40.0%
GAGAGAGAGAGAGAGAGATT+TGG - chr1_1:9924904-9924923 None:intergenic 45.0%
TGAAGTGCGAAAACATGTCG+GGG + chr1_1:9924940-9924959 Msa0006920:exon 45.0%
!!! GAGGTTTGCTGGTGTTTTAC+CGG - chr1_1:9924988-9925007 None:intergenic 45.0%
ACCAGCAAACCTCAAAGACA+AGG + chr1_1:9924995-9925014 Msa0006920:CDS 45.0%
!! TCCTTGTCTTTGAGGTTTGC+TGG - chr1_1:9924999-9925018 None:intergenic 45.0%
CGATGAAGAGAAAGGGTAAG+AGG - chr1_1:9925080-9925099 None:intergenic 45.0%
GTTTCATGGCGAATGATACG+CGG + chr1_1:9925564-9925583 Msa0006920:intron 45.0%
GCATATGTTCTCTGTGGTCA+TGG + chr1_1:9925943-9925962 Msa0006920:intron 45.0%
! TGGCCTTGGTGGATATTAGA+AGG + chr1_1:9926075-9926094 Msa0006920:intron 45.0%
! GGCCTTGGTGGATATTAGAA+GGG + chr1_1:9926076-9926095 Msa0006920:intron 45.0%
GGGAAATAATAGTTCCGGGA+GGG - chr1_1:9926119-9926138 None:intergenic 45.0%
AGGGAAATAATAGTTCCGGG+AGG - chr1_1:9926120-9926139 None:intergenic 45.0%
ATTCGGTTTAGCGCATTCGT+GGG - chr1_1:9926193-9926212 None:intergenic 45.0%
! AAGCGGCGAGTTGTTGATAA+CGG - chr1_1:9926368-9926387 None:intergenic 45.0%
TCAATATCGAAGTGGTTGGG+TGG + chr1_1:9926479-9926498 Msa0006920:intron 45.0%
CAATATCGAAGTGGTTGGGT+GGG + chr1_1:9926480-9926499 Msa0006920:intron 45.0%
AATATCGAAGTGGTTGGGTG+GGG + chr1_1:9926481-9926500 Msa0006920:intron 45.0%
!! TGAATGGGGTGGATTAGTGT+GGG + chr1_1:9926623-9926642 Msa0006920:intron 45.0%
ACTCGAGCTTCTATCCTCAT+AGG - chr1_1:9926871-9926890 None:intergenic 45.0%
TGTTGATGTGAGTCGAACCT+TGG + chr1_1:9926937-9926956 Msa0006920:intron 45.0%
!! CTTGGATGGTTTTTCAGAGG+GGG - chr1_1:9927051-9927070 None:intergenic 45.0%
!! GCTTGGATGGTTTTTCAGAG+GGG - chr1_1:9927052-9927071 None:intergenic 45.0%
!! GAGCTTGGATGGTTTTTCAG+AGG - chr1_1:9927054-9927073 None:intergenic 45.0%
CACATTGCTCACCTTATCAG+TGG - chr1_1:9927079-9927098 None:intergenic 45.0%
! TCATGTCAGGCTGCACTTTT+TGG - chr1_1:9927270-9927289 None:intergenic 45.0%
AAAGTGCAGCCTGACATGAA+AGG + chr1_1:9927271-9927290 Msa0006920:intron 45.0%
TGCAGCTTCTGGTAAGTCAT+CGG + chr1_1:9927295-9927314 Msa0006920:intron 45.0%
!! TCCATCGTCTTCTGAAGTCA+AGG + chr1_1:9927606-9927625 Msa0006920:CDS 45.0%
TCCTTGACTTCAGAAGACGA+TGG - chr1_1:9927610-9927629 None:intergenic 45.0%
AAGTCAAGGACAATGGCACA+TGG + chr1_1:9927620-9927639 Msa0006920:CDS 45.0%
ACAATGGCACATGGAAGAGT+TGG + chr1_1:9927629-9927648 Msa0006920:CDS 45.0%
CAATGGCACATGGAAGAGTT+GGG + chr1_1:9927630-9927649 Msa0006920:CDS 45.0%
AATGGCACATGGAAGAGTTG+GGG + chr1_1:9927631-9927650 Msa0006920:CDS 45.0%
AGTTTATTCTGCCGCTATCC+TGG + chr1_1:9927664-9927683 Msa0006920:CDS 45.0%
CCTCTGCAGTTAAATACTCC+AGG - chr1_1:9927685-9927704 None:intergenic 45.0%
! CCTGGAGTATTTAACTGCAG+AGG + chr1_1:9927682-9927701 Msa0006920:CDS 45.0%
CAATGCAAGCAAGGATCTGA+AGG + chr1_1:9927721-9927740 Msa0006920:CDS 45.0%
AAAGGAACAATAGCTGGTGG+TGG + chr1_1:9927812-9927831 Msa0006920:CDS 45.0%
TGGTATGGTACTGCATGTCT+AGG + chr1_1:9928075-9928094 Msa0006920:three_prime_UTR 45.0%
TGCGAAAACATGTCGGGGAA+AGG + chr1_1:9924945-9924964 Msa0006920:exon 50.0%
GCTCTTGAGGAACGAGATGT+GGG - chr1_1:9925032-9925051 None:intergenic 50.0%
AGCTCTTGAGGAACGAGATG+TGG - chr1_1:9925033-9925052 None:intergenic 50.0%
ACATCTCGTTCCTCAAGAGC+TGG + chr1_1:9925032-9925051 Msa0006920:CDS 50.0%
!! TTCCTCAAGAGCTGGTCTTC+AGG + chr1_1:9925040-9925059 Msa0006920:CDS 50.0%
AACCTGAAGACCAGCTCTTG+AGG - chr1_1:9925045-9925064 None:intergenic 50.0%
!! ACATGTGTCGGTGTTGTGTC+AGG + chr1_1:9925648-9925667 Msa0006920:intron 50.0%
!! CATGTGTCGGTGTTGTGTCA+GGG + chr1_1:9925649-9925668 Msa0006920:intron 50.0%
AGAAAATGCGCAAGTGGCCT+TGG + chr1_1:9926061-9926080 Msa0006920:intron 50.0%
TAATAGTTCCGGGAGGGCTA+TGG - chr1_1:9926113-9926132 None:intergenic 50.0%
TTCGGTTTAGCGCATTCGTG+GGG - chr1_1:9926192-9926211 None:intergenic 50.0%
CATTCGGTTTAGCGCATTCG+TGG - chr1_1:9926194-9926213 None:intergenic 50.0%
CACGAATGCGCTAAACCGAA+TGG + chr1_1:9926192-9926211 Msa0006920:intron 50.0%
CAGTGTGTGCTATCAAGGGA+TGG - chr1_1:9926412-9926431 None:intergenic 50.0%
ATATCGAAGTGGTTGGGTGG+GGG + chr1_1:9926482-9926501 Msa0006920:intron 50.0%
!! GTGAATGGGGTGGATTAGTG+TGG + chr1_1:9926622-9926641 Msa0006920:intron 50.0%
TATCAGTGGCAGAGCTTGGA+TGG - chr1_1:9927065-9927084 None:intergenic 50.0%
TCCAAGCTCTGCCACTGATA+AGG + chr1_1:9927065-9927084 Msa0006920:intron 50.0%
ACCTTATCAGTGGCAGAGCT+TGG - chr1_1:9927069-9927088 None:intergenic 50.0%
AAGCTGCAGCCTTTCATGTC+AGG - chr1_1:9927283-9927302 None:intergenic 50.0%
ACATGAAAGGCTGCAGCTTC+TGG + chr1_1:9927284-9927303 Msa0006920:intron 50.0%
!! AACTGCAGAGGTGTTGGAGT+TGG + chr1_1:9927694-9927713 Msa0006920:CDS 50.0%
!! CATCTACAGCTTGCTATCCG+TGG + chr1_1:9927764-9927783 Msa0006920:CDS 50.0%
GGATAATTCGCTCCTTGCGT+TGG + chr1_1:9927964-9927983 Msa0006920:three_prime_UTR 50.0%
ATGTCGGGGAAAGGAGCGAA+GGG + chr1_1:9924954-9924973 Msa0006920:CDS 55.0%
GGAGCGAAGGGTTTGACAAC+CGG + chr1_1:9924966-9924985 Msa0006920:CDS 55.0%
!! TGTCGGTGTTGTGTCAGGGT+TGG + chr1_1:9925653-9925672 Msa0006920:intron 55.0%
! AAATGCGCAAGTGGCCTTGG+TGG + chr1_1:9926064-9926083 Msa0006920:intron 55.0%
TCGGTTTAGCGCATTCGTGG+GGG - chr1_1:9926191-9926210 None:intergenic 55.0%
!! CGGCGAGTTGTTGATAACGG+TGG - chr1_1:9926365-9926384 None:intergenic 55.0%
GGCTGTAGCCGTAACACATG+TGG - chr1_1:9927340-9927359 None:intergenic 55.0%
GACGATGGATACGTCCGACA+GGG - chr1_1:9927595-9927614 None:intergenic 55.0%
AGACGATGGATACGTCCGAC+AGG - chr1_1:9927596-9927615 None:intergenic 55.0%
GTTGGCTGGCAATGCAAGCA+AGG + chr1_1:9927712-9927731 Msa0006920:CDS 55.0%
CATGTCGGGGAAAGGAGCGA+AGG + chr1_1:9924953-9924972 Msa0006920:exon 60.0%
!! GCAGAGGTGTTGGAGTTGGC+TGG + chr1_1:9927698-9927717 Msa0006920:CDS 60.0%
TAGGGTGGCCATAGCCCTCC+CGG + chr1_1:9926102-9926121 Msa0006920:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 9924886 9928191 9924886 ID=Msa0006920;Name=Msa0006920
chr1_1 mRNA 9924886 9928191 9924886 ID=Msa0006920-mRNA-1;Parent=Msa0006920;Name=Msa0006920-mRNA-1;_AED=0.27;_eAED=0.27;_QI=68|1|1|1|1|1|2|305|134
chr1_1 exon 9924886 9925061 9924886 ID=Msa0006920-mRNA-1:exon:3589;Parent=Msa0006920-mRNA-1
chr1_1 exon 9927590 9928191 9927590 ID=Msa0006920-mRNA-1:exon:3590;Parent=Msa0006920-mRNA-1
chr1_1 five_prime_UTR 9924886 9924953 9924886 ID=Msa0006920-mRNA-1:five_prime_utr;Parent=Msa0006920-mRNA-1
chr1_1 CDS 9924954 9925061 9924954 ID=Msa0006920-mRNA-1:cds;Parent=Msa0006920-mRNA-1
chr1_1 CDS 9927590 9927886 9927590 ID=Msa0006920-mRNA-1:cds;Parent=Msa0006920-mRNA-1
chr1_1 three_prime_UTR 9927887 9928191 9927887 ID=Msa0006920-mRNA-1:three_prime_utr;Parent=Msa0006920-mRNA-1
Gene Sequence

>Msa0006920

ATGTCGGGGAAAGGAGCGAAGGGTTTGACAACCGGTAAAACACCAGCAAACCTCAAAGACAAGGACAAGAAGAAACCCACATCTCGTTCCTCAAGAGCTGGTCTTCAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACAGCTGCAGTTTATTCTGCCGCTATCCTGGAGTATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGATGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA
Protein sequence

>Msa0006920

MSGKGAKGLTTGKTPANLKDKDKKKPTSRSSRAGLQFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE*