AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0620880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0620880 MtrunA17_Chr1g0148961 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0620880 MtrunA17_Chr1g0148951 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0620880 MtrunA17_Chr1g0184901 86.567 134 18 0 1 134 1 134 1.96e-73 215
Msa0620880 MtrunA17_Chr1g0211651 81.250 128 24 0 7 134 4 131 2.88e-71 209
Msa0620880 MtrunA17_Chr4g0024701 66.418 134 25 1 1 134 1 114 6.90e-47 147
Msa0620880 MtrunA17_Chr2g0319191 64.545 110 37 2 24 132 14 122 1.42e-40 132
Msa0620880 MtrunA17_Chr2g0319181 64.545 110 37 2 24 132 14 122 1.91e-40 131
Msa0620880 MtrunA17_Chr7g0268831 58.333 132 47 4 1 130 1 126 2.47e-40 131
Msa0620880 MtrunA17_Chr7g0273351 58.197 122 49 2 10 130 3 123 8.09e-40 130
Msa0620880 MtrunA17_Chr7g0273331 58.197 122 49 2 10 130 59 179 9.14e-39 129
Msa0620880 MtrunA17_Chr7g0259151 61.682 107 39 2 25 130 21 126 2.97e-38 126
Msa0620880 MtrunA17_Chr7g0272611 55.652 115 49 2 19 132 19 132 7.29e-37 123
Msa0620880 MtrunA17_Chr4g0031041 59.292 113 44 2 23 134 15 126 4.29e-36 121
Msa0620880 MtrunA17_Chr2g0327951 59.292 113 44 2 23 134 56 167 8.62e-36 121
Msa0620880 MtrunA17_Chr4g0035621 61.111 108 40 2 27 133 25 131 4.35e-35 118
Msa0620880 MtrunA17_Chr8g0379311 60.550 109 41 2 27 134 25 132 4.84e-35 118
Msa0620880 MtrunA17_Chr4g0032301 59.633 109 42 2 27 134 25 132 5.44e-35 118
Msa0620880 MtrunA17_Chr4g0031001 59.633 109 42 2 27 134 25 132 6.61e-35 118
Msa0620880 MtrunA17_Chr4g0031651 59.633 109 42 2 27 134 25 132 6.76e-35 118
Msa0620880 MtrunA17_Chr2g0327931 60.185 108 41 2 27 133 24 130 8.79e-35 118
Msa0620880 MtrunA17_Chr7g0273341 53.448 116 40 5 19 132 50 153 3.35e-28 101
Msa0620880 MtrunA17_Chr4g0031031 62.687 67 24 1 55 120 17 83 3.79e-20 79.3
Msa0620880 MtrunA17_Chr6g0449661 45.283 106 47 4 27 130 86 182 6.63e-19 78.6
Msa0620880 MtrunA17_Chr3g0119971 44.340 106 48 4 27 130 81 177 1.70e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0620880 AT1G52740.1 88.060 134 16 0 1 134 1 134 5.27e-76 222
Msa0620880 AT3G54560.1 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0620880 AT3G54560.2 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0620880 AT2G38810.1 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0620880 AT2G38810.4 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0620880 AT2G38810.3 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0620880 AT2G38810.2 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0620880 AT4G13570.1 66.667 111 33 1 18 124 8 118 3.28e-45 143
Msa0620880 AT4G13570.2 75.556 90 22 0 35 124 35 124 3.59e-44 141
Msa0620880 AT4G27230.2 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0620880 AT4G27230.1 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0620880 AT5G54640.1 64.545 110 37 2 24 132 14 122 2.45e-40 131
Msa0620880 AT3G20670.1 65.741 108 35 2 24 130 14 120 6.89e-40 130
Msa0620880 AT1G08880.1 64.762 105 35 2 25 128 21 124 1.01e-39 130
Msa0620880 AT1G51060.1 63.636 110 38 2 24 132 14 122 1.11e-39 130
Msa0620880 AT1G54690.1 63.810 105 36 2 25 128 21 124 2.89e-39 129
Msa0620880 AT5G02560.1 61.947 113 41 2 23 134 21 132 1.19e-38 128
Msa0620880 AT5G27670.1 60.870 115 43 2 19 132 18 131 4.54e-37 124
Msa0620880 AT5G59870.1 61.261 111 41 2 23 132 21 130 3.64e-36 121
Msa0620880 AT5G02560.2 51.095 137 41 3 23 134 21 156 3.79e-33 115

Find 54 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGTCTTTGAGGTTTGC+TGG 0.259581 4_2:+72668111 None:intergenic
TCTTCTTGTCCTTGTCTTTG+AGG 0.316058 4_2:+72668103 None:intergenic
TTCCTCAAGAGCTGGTCTTC+AGG 0.343744 4_2:-72668067 Msa0620880:intron
TATGAACATTCATTCCTTTG+AGG 0.376752 4_2:+72665239 None:intergenic
GGTGATAACAACTTTATTTG+TGG 0.407928 4_2:-72665169 Msa0620880:three_prime_UTR
CAATGGCACATGGAAGAGTT+GGG 0.442531 4_2:-72665482 Msa0620880:CDS
AGTTTATTCTGCCGCTATCC+TGG 0.451988 4_2:-72665448 Msa0620880:CDS
GTATTTAACTGCAGAGGTGT+TGG 0.470183 4_2:-72665424 Msa0620880:CDS
ACAATGGCACATGGAAGAGT+TGG 0.476378 4_2:-72665483 Msa0620880:CDS
GGAGCGAAGGGTTTGACAAC+CGG 0.477894 4_2:-72668141 Msa0620880:CDS
CTCATCAAAGGAACAATAGC+TGG 0.487626 4_2:-72665306 Msa0620880:CDS
TTGAAGTGCGAAAACATGTC+GGG 0.497395 4_2:-72668168 Msa0620880:exon
TGCATGTCTAGGATTTATGT+TGG 0.504995 4_2:-72665026 Msa0620880:three_prime_UTR
GGATAATTCGCTCCTTGCGT+TGG 0.505084 4_2:-72665148 Msa0620880:three_prime_UTR
ATAAGAGACTTGTGGATATG+AGG 0.507095 4_2:+72665270 None:intergenic
TGGTATGGTACTGCATGTCT+AGG 0.516636 4_2:-72665037 Msa0620880:three_prime_UTR
AGACGATGGATACGTCCGAC+AGG 0.539450 4_2:+72665519 None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG 0.540859 4_2:-72665418 Msa0620880:CDS
AGCTCTTGAGGAACGAGATG+TGG 0.542232 4_2:+72668077 None:intergenic
AACCTGAAGACCAGCTCTTG+AGG 0.543571 4_2:+72668065 None:intergenic
ACATCTCGTTCCTCAAGAGC+TGG 0.546311 4_2:-72668075 Msa0620880:CDS
TAATTCTCTCTAATTAACGC+AGG 0.546344 4_2:-72665218 Msa0620880:three_prime_UTR
GCAGAGGTGTTGGAGTTGGC+TGG 0.558822 4_2:-72665414 Msa0620880:CDS
CAATGCAAGCAAGGATCTGA+AGG 0.565081 4_2:-72665391 Msa0620880:CDS
TGCGAAAACATGTCGGGGAA+AGG 0.567757 4_2:-72668162 Msa0620880:exon
CCTCTGCAGTTAAATACTCC+AGG 0.571308 4_2:+72665430 None:intergenic
GAACTTGACACTCTCATCAA+AGG 0.571379 4_2:-72665318 Msa0620880:CDS
GTTGGCTGGCAATGCAAGCA+AGG 0.571379 4_2:-72665400 Msa0620880:CDS
AAATTCAGATAATCTAAAGA+AGG 0.572497 4_2:+72665107 None:intergenic
ACTTGTTGATAAGAGACTTG+TGG 0.572936 4_2:+72665262 None:intergenic
TCAGATAATCTAAAGAAGGA+TGG 0.582849 4_2:+72665111 None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG 0.584334 4_2:+72665354 None:intergenic
TCTTATCAACAAGTCCTCAA+AGG 0.584732 4_2:-72665253 Msa0620880:CDS
CATCTACAGCTTGCTATCCG+TGG 0.586408 4_2:-72665348 Msa0620880:CDS
TCCATCGTCTTCTGAAGTCA+AGG 0.589423 4_2:-72665506 Msa0620880:CDS
TTTGAAGTGCGAAAACATGT+CGG 0.591882 4_2:-72668169 Msa0620880:exon
CATGTCGGGGAAAGGAGCGA+AGG 0.599837 4_2:-72668154 Msa0620880:CDS
AATTCTCTCTAATTAACGCA+GGG 0.600377 4_2:-72665217 Msa0620880:three_prime_UTR
GCTCTTGAGGAACGAGATGT+GGG 0.607406 4_2:+72668078 None:intergenic
AAAGGAACAATAGCTGGTGG+TGG 0.608922 4_2:-72665300 Msa0620880:CDS
AAGTCAAGGACAATGGCACA+TGG 0.622887 4_2:-72665492 Msa0620880:CDS
ATGTCGGGGAAAGGAGCGAA+GGG 0.624226 4_2:-72668153 Msa0620880:CDS
CCTGGAGTATTTAACTGCAG+AGG 0.624849 4_2:-72665430 Msa0620880:CDS
CAATCAATCAAACCAACGCA+AGG 0.626065 4_2:+72665136 None:intergenic
GACGATGGATACGTCCGACA+GGG 0.626692 4_2:+72665520 None:intergenic
TTCTACATACAGTTCCCTGT+CGG 0.628096 4_2:-72665534 Msa0620880:intron
TGTCAAGTTCTTCATCTCCA+CGG 0.636093 4_2:+72665331 None:intergenic
ACCAGCAAACCTCAAAGACA+AGG 0.637885 4_2:-72668112 Msa0620880:CDS
AATGGCACATGGAAGAGTTG+GGG 0.645846 4_2:-72665481 Msa0620880:CDS
TGAAGTGCGAAAACATGTCG+GGG 0.649052 4_2:-72668167 Msa0620880:exon
TCTTCTGAAGTCAAGGACAA+TGG 0.655563 4_2:-72665499 Msa0620880:CDS
ATCAAAGGAACAATAGCTGG+TGG 0.661257 4_2:-72665303 Msa0620880:CDS
TCCTTGACTTCAGAAGACGA+TGG 0.668973 4_2:+72665505 None:intergenic
AGTTAAATACTCCAGGATAG+CGG 0.673071 4_2:+72665437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTATTTTGTTGTTAAA+AGG - chr4_2:72665295-72665314 Msa0620880:CDS 15.0%
!! ACAAAAAGAATAGTAAAAAA+CGG + chr4_2:72665579-72665598 None:intergenic 15.0%
!!! TTTATGATTTGTAATATCAA+TGG - chr4_2:72665910-72665929 Msa0620880:intron 15.0%
!!! TTTTTTTGTTTGTTTTAAGT+AGG - chr4_2:72666639-72666658 Msa0620880:intron 15.0%
!!! AGTATTATTTTATAACTGTT+TGG - chr4_2:72667081-72667100 Msa0620880:intron 15.0%
!!! AAAGGATTTTAATGTAATTT+CGG - chr4_2:72667978-72667997 Msa0620880:intron 15.0%
!!! TTTTGTTGTTAAAAGGATAA+TGG - chr4_2:72665302-72665321 Msa0620880:CDS 20.0%
!!! TTTGTTGTTAAAAGGATAAT+GGG - chr4_2:72665303-72665322 Msa0620880:CDS 20.0%
!!! AAAAATTGTAGTTTGAGATT+TGG - chr4_2:72665343-72665362 Msa0620880:CDS 20.0%
!!! AAAATTGTAGTTTGAGATTT+GGG - chr4_2:72665344-72665363 Msa0620880:CDS 20.0%
!!! TTTTTATCTAAGATGAAGTA+TGG - chr4_2:72665367-72665386 Msa0620880:CDS 20.0%
!!! TTTTATCTAAGATGAAGTAT+GGG - chr4_2:72665368-72665387 Msa0620880:CDS 20.0%
!!! TTTTTACCAAGATTGAAATT+TGG - chr4_2:72665406-72665425 Msa0620880:CDS 20.0%
!!! TTTTACCAAGATTGAAATTT+GGG - chr4_2:72665407-72665426 Msa0620880:CDS 20.0%
!! ATATATACACCTATTAAGAA+GGG + chr4_2:72666195-72666214 None:intergenic 20.0%
!! AATATATACACCTATTAAGA+AGG + chr4_2:72666196-72666215 None:intergenic 20.0%
!! ATATATTCAGTAATGCTAAA+TGG - chr4_2:72666209-72666228 Msa0620880:intron 20.0%
!! ATTATAAAAGATAGTCCATT+CGG + chr4_2:72666266-72666285 None:intergenic 20.0%
!! TGGAAAATGAAAAGATAATT+TGG + chr4_2:72666346-72666365 None:intergenic 20.0%
!!! CTTCGATATTGATTAATTAA+TGG + chr4_2:72666527-72666546 None:intergenic 20.0%
!! TTAATTAATCAATATCGAAG+TGG - chr4_2:72666527-72666546 Msa0620880:intron 20.0%
!! TTATAGAATCATATGTGAAT+GGG - chr4_2:72666664-72666683 Msa0620880:intron 20.0%
!! TATTGTATTTCTTTGATTCT+TGG - chr4_2:72667492-72667511 Msa0620880:intron 20.0%
!! AAATTCAGATAATCTAAAGA+AGG + chr4_2:72668064-72668083 None:intergenic 20.0%
!!! ATTTTTCTTCTCTTTTGATA+TGG - chr4_2:72668080-72668099 Msa0620880:CDS 20.0%
! AGTGAAAAAGGTTAAATTTG+AGG + chr4_2:72665191-72665210 None:intergenic 25.0%
!! TTTATCTAAGATGAAGTATG+GGG - chr4_2:72665369-72665388 Msa0620880:CDS 25.0%
! CAGTGAAAAACTAATAACAT+GGG + chr4_2:72665486-72665505 None:intergenic 25.0%
! TCAGTGAAAAACTAATAACA+TGG + chr4_2:72665487-72665506 None:intergenic 25.0%
! CAGTTAAGCAAATTGTAAAT+GGG + chr4_2:72665510-72665529 None:intergenic 25.0%
! TCAGTTAAGCAAATTGTAAA+TGG + chr4_2:72665511-72665530 None:intergenic 25.0%
! AAAAGAATAGTAAAAAACGG+TGG + chr4_2:72665576-72665595 None:intergenic 25.0%
!!! AATTACCTTGTTTTGTTTCA+TGG - chr4_2:72665606-72665625 Msa0620880:intron 25.0%
! GAGAATTGAATAGTTGAATA+TGG - chr4_2:72665679-72665698 Msa0620880:intron 25.0%
!!! GGATGTTCATTTTATTTCTT+TGG - chr4_2:72665812-72665831 Msa0620880:intron 25.0%
! CAAAAGAAGAAATCAAATCA+TGG + chr4_2:72665857-72665876 None:intergenic 25.0%
!! GCAATTCATTTTACAACTTA+AGG + chr4_2:72665949-72665968 None:intergenic 25.0%
! AGAGAACATATGCAAAATAA+GGG + chr4_2:72665992-72666011 None:intergenic 25.0%
! TTGTTTGAAAAACTTAGACA+AGG + chr4_2:72666026-72666045 None:intergenic 25.0%
!!! TTTTCTTTTTTCGGGAAAAA+AGG + chr4_2:72666103-72666122 None:intergenic 25.0%
! CTATTATTTCCCTTCTTAAT+AGG - chr4_2:72666183-72666202 Msa0620880:intron 25.0%
!!! AAAAGATAATTTGGTGTAAG+GGG + chr4_2:72666337-72666356 None:intergenic 25.0%
!!! GAAAAGATAATTTGGTGTAA+GGG + chr4_2:72666338-72666357 None:intergenic 25.0%
!!! TGAAAAGATAATTTGGTGTA+AGG + chr4_2:72666339-72666358 None:intergenic 25.0%
! CAAAAAAACAATCAATCCAT+TGG + chr4_2:72666627-72666646 None:intergenic 25.0%
! GTTATAGAATCATATGTGAA+TGG - chr4_2:72666663-72666682 Msa0620880:intron 25.0%
! TATAGAATCATATGTGAATG+GGG - chr4_2:72666665-72666684 Msa0620880:intron 25.0%
!! AGTTATCACATAATCTTTTG+TGG - chr4_2:72666875-72666894 Msa0620880:intron 25.0%
!! ACAGATTTTCACATATACAT+GGG + chr4_2:72667162-72667181 None:intergenic 25.0%
!! AACAGATTTTCACATATACA+TGG + chr4_2:72667163-72667182 None:intergenic 25.0%
! AAAAAGACAAAATCATCACA+CGG + chr4_2:72667443-72667462 None:intergenic 25.0%
AAGAAAACGATGAAGAGAAA+GGG + chr4_2:72665148-72665167 None:intergenic 30.0%
CCAAAACAAAAGAGTGAAAA+AGG + chr4_2:72665203-72665222 None:intergenic 30.0%
!! CCTTTTTCACTCTTTTGTTT+TGG - chr4_2:72665200-72665219 Msa0620880:three_prime_UTR 30.0%
GATTTATTGTGTCACATTGA+TGG - chr4_2:72665529-72665548 Msa0620880:intron 30.0%
CTTTAGATTAAAGACGTCTT+CGG + chr4_2:72665771-72665790 None:intergenic 30.0%
! ACGTCTTTAATCTAAAGTGT+TGG - chr4_2:72665774-72665793 Msa0620880:intron 30.0%
!! TGTTGGTGCTACATTATTTA+TGG - chr4_2:72665791-72665810 Msa0620880:intron 30.0%
!! AAATGAATTGCTCACTTTTG+AGG - chr4_2:72665958-72665977 Msa0620880:intron 30.0%
CAGAGAACATATGCAAAATA+AGG + chr4_2:72665993-72666012 None:intergenic 30.0%
! TATTTTGCATATGTTCTCTG+TGG - chr4_2:72665993-72666012 Msa0620880:intron 30.0%
! ACTTGCGCATTTTCTTTTTT+CGG + chr4_2:72666112-72666131 None:intergenic 30.0%
TGGATATTAGAAGGGATATA+GGG - chr4_2:72666140-72666159 Msa0620880:intron 30.0%
AAGAAGGGAAATAATAGTTC+CGG + chr4_2:72666180-72666199 None:intergenic 30.0%
GATTTCAATCCAATGATCAT+TGG + chr4_2:72666497-72666516 None:intergenic 30.0%
TTAATCAATATCGAAGTGGT+TGG - chr4_2:72666531-72666550 Msa0620880:intron 30.0%
TAATCAATATCGAAGTGGTT+GGG - chr4_2:72666532-72666551 Msa0620880:intron 30.0%
TTGCAGTCAAGATTAGTAAT+TGG - chr4_2:72666585-72666604 Msa0620880:intron 30.0%
! ATGGATTACATAAAACACAG+AGG + chr4_2:72667266-72667285 None:intergenic 30.0%
TTAGTAATCCAAAAACGAGA+AGG + chr4_2:72667292-72667311 None:intergenic 30.0%
!! TCGTTTTTGGATTACTAATG+TGG - chr4_2:72667294-72667313 Msa0620880:intron 30.0%
GTAAATCATGTTGCATTTGT+TGG - chr4_2:72667559-72667578 Msa0620880:intron 30.0%
ATTTATCCCACATCATAATC+TGG - chr4_2:72667592-72667611 Msa0620880:intron 30.0%
ATGTAGAAAGATGTTCATGA+TGG + chr4_2:72667622-72667641 None:intergenic 30.0%
TATGAACATTCATTCCTTTG+AGG + chr4_2:72667932-72667951 None:intergenic 30.0%
TAATTCTCTCTAATTAACGC+AGG - chr4_2:72667950-72667969 Msa0620880:intron 30.0%
AATTCTCTCTAATTAACGCA+GGG - chr4_2:72667951-72667970 Msa0620880:intron 30.0%
TAATTAACGCAGGGAAAAAA+AGG - chr4_2:72667960-72667979 Msa0620880:intron 30.0%
! GGTGATAACAACTTTATTTG+TGG - chr4_2:72667999-72668018 Msa0620880:intron 30.0%
TCAGATAATCTAAAGAAGGA+TGG + chr4_2:72668060-72668079 None:intergenic 30.0%
!! AACTAACATCTTCACTTTTG+TGG - chr4_2:72668111-72668130 Msa0620880:CDS 30.0%
!! TCTATCTGTTTTGAATTGCT+TGG - chr4_2:72668185-72668204 Msa0620880:five_prime_UTR 30.0%
!!! AGAGAGATATTTGGCTTTTG+AGG + chr4_2:72664952-72664971 None:intergenic 35.0%
! TTTGAAGTGCGAAAACATGT+CGG - chr4_2:72664999-72665018 Msa0620880:three_prime_UTR 35.0%
CAAGAAAACGATGAAGAGAA+AGG + chr4_2:72665149-72665168 None:intergenic 35.0%
CACTTTAGCGATGTAACATA+AGG + chr4_2:72665249-72665268 None:intergenic 35.0%
GTACACCCAAATTTCAATCT+TGG + chr4_2:72665415-72665434 None:intergenic 35.0%
ATTCGCCATGAAACAAAACA+AGG + chr4_2:72665614-72665633 None:intergenic 35.0%
TTGAATATGGTCACATGTGT+CGG - chr4_2:72665692-72665711 Msa0620880:intron 35.0%
! CTTGCGCATTTTCTTTTTTC+GGG + chr4_2:72666111-72666130 None:intergenic 35.0%
GAAAAAAGAAAATGCGCAAG+TGG - chr4_2:72666111-72666130 Msa0620880:intron 35.0%
GTGGATATTAGAAGGGATAT+AGG - chr4_2:72666139-72666158 Msa0620880:intron 35.0%
ATATTAGAAGGGATATAGGG+TGG - chr4_2:72666143-72666162 Msa0620880:intron 35.0%
AGAAGGGAAATAATAGTTCC+GGG + chr4_2:72666179-72666198 None:intergenic 35.0%
TAAAGCTTGTACAGTGTAAG+CGG + chr4_2:72666441-72666460 None:intergenic 35.0%
CTGTCATAACCAATGATCAT+TGG - chr4_2:72666485-72666504 Msa0620880:intron 35.0%
!!! TGTTTTAACTGTCAAGACCA+AGG + chr4_2:72667013-72667032 None:intergenic 35.0%
ATGCTGTGTTCAATTAGACA+TGG + chr4_2:72667204-72667223 None:intergenic 35.0%
TCTGCATTACTTATTCTGTG+CGG - chr4_2:72667230-72667249 Msa0620880:intron 35.0%
! TCCATATTCCTTCTCGTTTT+TGG - chr4_2:72667281-72667300 Msa0620880:intron 35.0%
TCCAAAAACGAGAAGGAATA+TGG + chr4_2:72667285-72667304 None:intergenic 35.0%
CCACTTGCAAAAAATTCTAG+TGG + chr4_2:72667467-72667486 None:intergenic 35.0%
!!! CCACTAGAATTTTTTGCAAG+TGG - chr4_2:72667464-72667483 Msa0620880:intron 35.0%
AAGCATGCTAAAATGTTGTC+TGG - chr4_2:72667534-72667553 Msa0620880:intron 35.0%
GGAAAACCAGATTATGATGT+GGG + chr4_2:72667601-72667620 None:intergenic 35.0%
TGGAAAACCAGATTATGATG+TGG + chr4_2:72667602-72667621 None:intergenic 35.0%
AGTTAAATACTCCAGGATAG+CGG + chr4_2:72667734-72667753 None:intergenic 35.0%
ATAAGAGACTTGTGGATATG+AGG + chr4_2:72667901-72667920 None:intergenic 35.0%
! ACTTGTTGATAAGAGACTTG+TGG + chr4_2:72667909-72667928 None:intergenic 35.0%
TCTTATCAACAAGTCCTCAA+AGG - chr4_2:72667915-72667934 Msa0620880:intron 35.0%
!! ACATCTTCACTTTTGTGGTA+TGG - chr4_2:72668116-72668135 Msa0620880:CDS 35.0%
! TGCATGTCTAGGATTTATGT+TGG - chr4_2:72668142-72668161 Msa0620880:CDS 35.0%
!! TGTTTTGAATTGCTTGGCAA+TGG - chr4_2:72668191-72668210 Msa0620880:five_prime_UTR 35.0%
GAGAGAGAGAGAGAGATATT+TGG + chr4_2:72664961-72664980 None:intergenic 40.0%
TTGAAGTGCGAAAACATGTC+GGG - chr4_2:72665000-72665019 Msa0620880:three_prime_UTR 40.0%
TCTTCTTGTCCTTGTCTTTG+AGG + chr4_2:72665068-72665087 None:intergenic 40.0%
GATGAAGAGAAAGGGTAAGA+GGG + chr4_2:72665140-72665159 None:intergenic 40.0%
AGATGAAGTATGGGGTAGAA+AGG - chr4_2:72665377-72665396 Msa0620880:CDS 40.0%
!! TGTCACATTGATGGTGTCTT+TGG - chr4_2:72665538-72665557 Msa0620880:intron 40.0%
!! ACATTGATGGTGTCTTTGGA+AGG - chr4_2:72665542-72665561 Msa0620880:intron 40.0%
!! CATTGATGGTGTCTTTGGAA+GGG - chr4_2:72665543-72665562 Msa0620880:intron 40.0%
!! ATTGATGGTGTCTTTGGAAG+GGG - chr4_2:72665544-72665563 Msa0620880:intron 40.0%
! CTTCGGTGTAAAACATGTGA+TGG + chr4_2:72665754-72665773 None:intergenic 40.0%
TTGGACTTCTAACAGAGACT+AGG - chr4_2:72665831-72665850 Msa0620880:intron 40.0%
CATATGTTCTCTGTGGTCAT+GGG - chr4_2:72666000-72666019 Msa0620880:intron 40.0%
ATCCCTTCTAATATCCACCA+AGG + chr4_2:72666137-72666156 None:intergenic 40.0%
TATTAGCTAACTATGCGGTG+TGG + chr4_2:72666366-72666385 None:intergenic 40.0%
ACACCGCATAGTTAGCTAAT+AGG - chr4_2:72666365-72666384 Msa0620880:intron 40.0%
CTGCCTATTAGCTAACTATG+CGG + chr4_2:72666371-72666390 None:intergenic 40.0%
ATGACAGTGTGTGCTATCAA+GGG + chr4_2:72666472-72666491 None:intergenic 40.0%
TATGACAGTGTGTGCTATCA+AGG + chr4_2:72666473-72666492 None:intergenic 40.0%
CTTGACTGCAACGTAAAAGA+TGG + chr4_2:72666576-72666595 None:intergenic 40.0%
ATGCTTCACTCTCAAACCAA+TGG - chr4_2:72666608-72666627 Msa0620880:intron 40.0%
AGAATCATATGTGAATGGGG+TGG - chr4_2:72666668-72666687 Msa0620880:intron 40.0%
! CATAATCTTTTGTGGTCCAG+TGG - chr4_2:72666883-72666902 Msa0620880:intron 40.0%
CCAAAAAAGCAGATTACCAC+TGG + chr4_2:72666902-72666921 None:intergenic 40.0%
!!! CCAGTGGTAATCTGCTTTTT+TGG - chr4_2:72666899-72666918 Msa0620880:intron 40.0%
!!! CTGCTTTTTTGGTTCCTATG+AGG - chr4_2:72666910-72666929 Msa0620880:intron 40.0%
!! AGCTTGGATGGTTTTTCAGA+GGG + chr4_2:72667109-72667128 None:intergenic 40.0%
TGCTTCATCCACATGTGTTA+CGG - chr4_2:72667385-72667404 Msa0620880:intron 40.0%
CCAAAGATAAGTCACTCACA+TGG + chr4_2:72667417-72667436 None:intergenic 40.0%
CCATGTGAGTGACTTATCTT+TGG - chr4_2:72667414-72667433 Msa0620880:intron 40.0%
TTCTACATACAGTTCCCTGT+CGG - chr4_2:72667634-72667653 Msa0620880:intron 40.0%
!! TCTTCTGAAGTCAAGGACAA+TGG - chr4_2:72667669-72667688 Msa0620880:intron 40.0%
! GTATTTAACTGCAGAGGTGT+TGG - chr4_2:72667744-72667763 Msa0620880:intron 40.0%
ATAGCAAGCTGTAGATGTCT+CGG + chr4_2:72667817-72667836 None:intergenic 40.0%
TGTCAAGTTCTTCATCTCCA+CGG + chr4_2:72667840-72667859 None:intergenic 40.0%
GAACTTGACACTCTCATCAA+AGG - chr4_2:72667850-72667869 Msa0620880:intron 40.0%
CTCATCAAAGGAACAATAGC+TGG - chr4_2:72667862-72667881 Msa0620880:intron 40.0%
ATCAAAGGAACAATAGCTGG+TGG - chr4_2:72667865-72667884 Msa0620880:intron 40.0%
CAATCAATCAAACCAACGCA+AGG + chr4_2:72668035-72668054 None:intergenic 40.0%
! AATGCAAAGTCTTTTGCTGC+TGG - chr4_2:72668217-72668236 Msa0620880:five_prime_UTR 40.0%
TGAAGTGCGAAAACATGTCG+GGG - chr4_2:72665001-72665020 Msa0620880:three_prime_UTR 45.0%
!!! GAGGTTTGCTGGTGTTTTAC+CGG + chr4_2:72665049-72665068 None:intergenic 45.0%
ACCAGCAAACCTCAAAGACA+AGG - chr4_2:72665056-72665075 Msa0620880:three_prime_UTR 45.0%
!! TCCTTGTCTTTGAGGTTTGC+TGG + chr4_2:72665060-72665079 None:intergenic 45.0%
CGATGAAGAGAAAGGGTAAG+AGG + chr4_2:72665141-72665160 None:intergenic 45.0%
GTTTCATGGCGAATGATACG+CGG - chr4_2:72665620-72665639 Msa0620880:intron 45.0%
GCATATGTTCTCTGTGGTCA+TGG - chr4_2:72665999-72666018 Msa0620880:intron 45.0%
! TGGCCTTGGTGGATATTAGA+AGG - chr4_2:72666131-72666150 Msa0620880:intron 45.0%
! GGCCTTGGTGGATATTAGAA+GGG - chr4_2:72666132-72666151 Msa0620880:intron 45.0%
GGGAAATAATAGTTCCGGGA+GGG + chr4_2:72666175-72666194 None:intergenic 45.0%
AGGGAAATAATAGTTCCGGG+AGG + chr4_2:72666176-72666195 None:intergenic 45.0%
ATTCGGTTTAGCGCATTCGT+GGG + chr4_2:72666249-72666268 None:intergenic 45.0%
! AAGCGGCGAGTTGTTGATAA+CGG + chr4_2:72666424-72666443 None:intergenic 45.0%
TCAATATCGAAGTGGTTGGG+TGG - chr4_2:72666535-72666554 Msa0620880:intron 45.0%
CAATATCGAAGTGGTTGGGT+GGG - chr4_2:72666536-72666555 Msa0620880:intron 45.0%
AATATCGAAGTGGTTGGGTG+GGG - chr4_2:72666537-72666556 Msa0620880:intron 45.0%
!! TGAATGGGGTGGATTAGTGT+GGG - chr4_2:72666679-72666698 Msa0620880:intron 45.0%
ACTCGAGCTTCTATCCTCAT+AGG + chr4_2:72666927-72666946 None:intergenic 45.0%
TGTTGATGTGAGTCGAACCT+TGG - chr4_2:72666993-72667012 Msa0620880:intron 45.0%
!! CTTGGATGGTTTTTCAGAGG+GGG + chr4_2:72667107-72667126 None:intergenic 45.0%
!! GCTTGGATGGTTTTTCAGAG+GGG + chr4_2:72667108-72667127 None:intergenic 45.0%
!! GAGCTTGGATGGTTTTTCAG+AGG + chr4_2:72667110-72667129 None:intergenic 45.0%
CACATTGCTCACCTTATCAG+TGG + chr4_2:72667135-72667154 None:intergenic 45.0%
! TCATGTCAGGCTGCACTTTT+TGG + chr4_2:72667326-72667345 None:intergenic 45.0%
AAAGTGCAGCCTGACATGAA+AGG - chr4_2:72667327-72667346 Msa0620880:intron 45.0%
TGCAGCTTCTGGTAAGTCAT+CGG - chr4_2:72667351-72667370 Msa0620880:intron 45.0%
!! TCCATCGTCTTCTGAAGTCA+AGG - chr4_2:72667662-72667681 Msa0620880:intron 45.0%
TCCTTGACTTCAGAAGACGA+TGG + chr4_2:72667666-72667685 None:intergenic 45.0%
AAGTCAAGGACAATGGCACA+TGG - chr4_2:72667676-72667695 Msa0620880:intron 45.0%
ACAATGGCACATGGAAGAGT+TGG - chr4_2:72667685-72667704 Msa0620880:intron 45.0%
CAATGGCACATGGAAGAGTT+GGG - chr4_2:72667686-72667705 Msa0620880:intron 45.0%
AATGGCACATGGAAGAGTTG+GGG - chr4_2:72667687-72667706 Msa0620880:intron 45.0%
AGTTTATTCTGCCGCTATCC+TGG - chr4_2:72667720-72667739 Msa0620880:intron 45.0%
CCTCTGCAGTTAAATACTCC+AGG + chr4_2:72667741-72667760 None:intergenic 45.0%
! CCTGGAGTATTTAACTGCAG+AGG - chr4_2:72667738-72667757 Msa0620880:intron 45.0%
CAATGCAAGCAAGGATCTGA+AGG - chr4_2:72667777-72667796 Msa0620880:intron 45.0%
AAAGGAACAATAGCTGGTGG+TGG - chr4_2:72667868-72667887 Msa0620880:intron 45.0%
TGGTATGGTACTGCATGTCT+AGG - chr4_2:72668131-72668150 Msa0620880:CDS 45.0%
TGCGAAAACATGTCGGGGAA+AGG - chr4_2:72665006-72665025 Msa0620880:three_prime_UTR 50.0%
GCTCTTGAGGAACGAGATGT+GGG + chr4_2:72665093-72665112 None:intergenic 50.0%
AGCTCTTGAGGAACGAGATG+TGG + chr4_2:72665094-72665113 None:intergenic 50.0%
ACATCTCGTTCCTCAAGAGC+TGG - chr4_2:72665093-72665112 Msa0620880:three_prime_UTR 50.0%
!! TTCCTCAAGAGCTGGTCTTC+AGG - chr4_2:72665101-72665120 Msa0620880:three_prime_UTR 50.0%
AACCTGAAGACCAGCTCTTG+AGG + chr4_2:72665106-72665125 None:intergenic 50.0%
!! ACATGTGTCGGTGTTGTGTC+AGG - chr4_2:72665704-72665723 Msa0620880:intron 50.0%
!! CATGTGTCGGTGTTGTGTCA+GGG - chr4_2:72665705-72665724 Msa0620880:intron 50.0%
AGAAAATGCGCAAGTGGCCT+TGG - chr4_2:72666117-72666136 Msa0620880:intron 50.0%
TAATAGTTCCGGGAGGGCTA+TGG + chr4_2:72666169-72666188 None:intergenic 50.0%
TTCGGTTTAGCGCATTCGTG+GGG + chr4_2:72666248-72666267 None:intergenic 50.0%
CATTCGGTTTAGCGCATTCG+TGG + chr4_2:72666250-72666269 None:intergenic 50.0%
CACGAATGCGCTAAACCGAA+TGG - chr4_2:72666248-72666267 Msa0620880:intron 50.0%
CAGTGTGTGCTATCAAGGGA+TGG + chr4_2:72666468-72666487 None:intergenic 50.0%
ATATCGAAGTGGTTGGGTGG+GGG - chr4_2:72666538-72666557 Msa0620880:intron 50.0%
!! GTGAATGGGGTGGATTAGTG+TGG - chr4_2:72666678-72666697 Msa0620880:intron 50.0%
TATCAGTGGCAGAGCTTGGA+TGG + chr4_2:72667121-72667140 None:intergenic 50.0%
TCCAAGCTCTGCCACTGATA+AGG - chr4_2:72667121-72667140 Msa0620880:intron 50.0%
ACCTTATCAGTGGCAGAGCT+TGG + chr4_2:72667125-72667144 None:intergenic 50.0%
AAGCTGCAGCCTTTCATGTC+AGG + chr4_2:72667339-72667358 None:intergenic 50.0%
ACATGAAAGGCTGCAGCTTC+TGG - chr4_2:72667340-72667359 Msa0620880:intron 50.0%
!! AACTGCAGAGGTGTTGGAGT+TGG - chr4_2:72667750-72667769 Msa0620880:intron 50.0%
!! CATCTACAGCTTGCTATCCG+TGG - chr4_2:72667820-72667839 Msa0620880:intron 50.0%
GGATAATTCGCTCCTTGCGT+TGG - chr4_2:72668020-72668039 Msa0620880:intron 50.0%
ATGTCGGGGAAAGGAGCGAA+GGG - chr4_2:72665015-72665034 Msa0620880:three_prime_UTR 55.0%
GGAGCGAAGGGTTTGACAAC+CGG - chr4_2:72665027-72665046 Msa0620880:three_prime_UTR 55.0%
!! TGTCGGTGTTGTGTCAGGGT+TGG - chr4_2:72665709-72665728 Msa0620880:intron 55.0%
! AAATGCGCAAGTGGCCTTGG+TGG - chr4_2:72666120-72666139 Msa0620880:intron 55.0%
TCGGTTTAGCGCATTCGTGG+GGG + chr4_2:72666247-72666266 None:intergenic 55.0%
!! CGGCGAGTTGTTGATAACGG+TGG + chr4_2:72666421-72666440 None:intergenic 55.0%
GGCTGTAGCCGTAACACATG+TGG + chr4_2:72667396-72667415 None:intergenic 55.0%
GACGATGGATACGTCCGACA+GGG + chr4_2:72667651-72667670 None:intergenic 55.0%
AGACGATGGATACGTCCGAC+AGG + chr4_2:72667652-72667671 None:intergenic 55.0%
GTTGGCTGGCAATGCAAGCA+AGG - chr4_2:72667768-72667787 Msa0620880:intron 55.0%
CATGTCGGGGAAAGGAGCGA+AGG - chr4_2:72665014-72665033 Msa0620880:three_prime_UTR 60.0%
!! GCAGAGGTGTTGGAGTTGGC+TGG - chr4_2:72667754-72667773 Msa0620880:intron 60.0%
TAGGGTGGCCATAGCCCTCC+CGG - chr4_2:72666158-72666177 Msa0620880:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 72664943 72668247 72664943 ID=Msa0620880;Name=Msa0620880
chr4_2 mRNA 72664943 72668247 72664943 ID=Msa0620880-mRNA-1;Parent=Msa0620880;Name=Msa0620880-mRNA-1;_AED=0.27;_eAED=0.27;_QI=72|1|1|1|1|1|2|305|134
chr4_2 exon 72664943 72665544 72664943 ID=Msa0620880-mRNA-1:exon:21703;Parent=Msa0620880-mRNA-1
chr4_2 exon 72668068 72668247 72668068 ID=Msa0620880-mRNA-1:exon:21702;Parent=Msa0620880-mRNA-1
chr4_2 five_prime_UTR 72668176 72668247 72668176 ID=Msa0620880-mRNA-1:five_prime_utr;Parent=Msa0620880-mRNA-1
chr4_2 CDS 72668068 72668175 72668068 ID=Msa0620880-mRNA-1:cds;Parent=Msa0620880-mRNA-1
chr4_2 CDS 72665248 72665544 72665248 ID=Msa0620880-mRNA-1:cds;Parent=Msa0620880-mRNA-1
chr4_2 three_prime_UTR 72664943 72665247 72664943 ID=Msa0620880-mRNA-1:three_prime_utr;Parent=Msa0620880-mRNA-1
Gene Sequence

>Msa0620880

ATGTCGGGGAAAGGAGCGAAGGGTTTGACAACCGGTAAAACACCAGCAAACCTCAAAGACAAGGACAAGAAGAAACCCACATCTCGTTCCTCAAGAGCTGGTCTTCAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACAGCTGCAGTTTATTCTGCCGCTATCCTGGAGTATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGATGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA
Protein sequence

>Msa0620880

MSGKGAKGLTTGKTPANLKDKDKKKPTSRSSRAGLQFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE*