AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0014090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0014090 MtrunA17_Chr1g0164131 79.032 62 13 0 1 62 484 545 1.31e-26 103
Msa0014090 MtrunA17_Chr1g0164131 61.644 73 23 1 73 145 285 352 1.65e-17 78.2
Msa0014090 MtrunA17_Chr1g0164031 44.853 136 55 3 26 146 6 136 3.62e-21 87.0
Msa0014090 MtrunA17_Chr1g0163811 54.839 62 23 1 84 145 90 146 3.84e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0014090 AT1G66345.1 29.655 145 71 2 1 145 240 353 6.70e-11 59.3

Find 19 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGCTGACAAGTATTTA+AGG 0.159059 1_1:+22143090 Msa0014090:CDS
TAAAGTTAGGTTAGGCGATT+TGG 0.312974 1_1:+22144432 Msa0014090:intron
ACATAAACAATCAACAAATA+AGG 0.330227 1_1:-22144409 None:intergenic
AAGTATTTAAGGACTATAAA+AGG 0.339479 1_1:+22143101 Msa0014090:CDS
ATTCAGTGTTTGTGCCGTTT+TGG 0.340824 1_1:+22143059 Msa0014090:CDS
TCACAATGAATTGGCCTCAT+TGG 0.342113 1_1:+22143211 Msa0014090:CDS
GAAGAATTTGATTGGTGATT+CGG 0.368165 1_1:+22144384 Msa0014090:CDS
TCTTCAGCTTCACAATGAAT+TGG 0.380128 1_1:+22143202 Msa0014090:CDS
GTCTCATATATACTCACATT+TGG 0.390610 1_1:-22143137 None:intergenic
TTTCATCATTTCCCTTCTGC+AGG 0.399952 1_1:-22143175 None:intergenic
GCAGAAGGGAAATGATGAAA+GGG 0.515847 1_1:+22143178 Msa0014090:CDS
TTGTTGCAGAAGAATTTGAT+TGG 0.523742 1_1:+22144376 Msa0014090:CDS
TGCAGAAGGGAAATGATGAA+AGG 0.551649 1_1:+22143177 Msa0014090:CDS
GATCGCTGGTCACCTGCAGA+AGG 0.557140 1_1:+22143163 Msa0014090:CDS
TTGGAGATGATGTTGAGAAG+AGG 0.579266 1_1:+22144496 Msa0014090:CDS
ATATATGAGACATTGATCGC+TGG 0.614340 1_1:+22143149 Msa0014090:CDS
ATCGCTGGTCACCTGCAGAA+GGG 0.624789 1_1:+22143164 Msa0014090:CDS
ACTATGTTGCAACTCCAATG+AGG 0.668614 1_1:-22143225 None:intergenic
ATAAAACACTCGTATAAACG+AGG 0.746007 1_1:-22144545 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTAAATGTTAGTTA+TGG + chr1_1:22143970-22143989 Msa0014090:intron 10.0%
!! ATTAAATTAAATGTTAGTTA+TGG + chr1_1:22143970-22143989 Msa0014090:intron 10.0%
!!! AGATGTATACATTATTTTTT+TGG + chr1_1:22144467-22144486 Msa0014090:CDS 15.0%
!!! ATTATTTTTTTGGTAGAAAT+TGG + chr1_1:22144477-22144496 Msa0014090:intron 15.0%
!!! AGATGTATACATTATTTTTT+TGG + chr1_1:22144467-22144486 Msa0014090:CDS 15.0%
!!! ATTATTTTTTTGGTAGAAAT+TGG + chr1_1:22144477-22144496 Msa0014090:intron 15.0%
!! AAGTATTTAAGGACTATAAA+AGG + chr1_1:22143101-22143120 Msa0014090:CDS 20.0%
!! ACATAAACAATCAACAAATA+AGG - chr1_1:22144412-22144431 None:intergenic 20.0%
!! TGATTGTTTATGTTAAAGTT+AGG + chr1_1:22144419-22144438 Msa0014090:intron 20.0%
!! AAGTATTTAAGGACTATAAA+AGG + chr1_1:22143101-22143120 Msa0014090:CDS 20.0%
!! ACATAAACAATCAACAAATA+AGG - chr1_1:22144412-22144431 None:intergenic 20.0%
!! TGATTGTTTATGTTAAAGTT+AGG + chr1_1:22144419-22144438 Msa0014090:intron 20.0%
! AAAATGCTGACAAGTATTTA+AGG + chr1_1:22143090-22143109 Msa0014090:CDS 25.0%
!!! TACTAAGTTTTAAGCAACTT+CGG + chr1_1:22143296-22143315 Msa0014090:intron 25.0%
! CATTTGATTGAATTAAACCT+TGG + chr1_1:22143560-22143579 Msa0014090:intron 25.0%
! TCATTATCCAAATTCACTTA+AGG + chr1_1:22143618-22143637 Msa0014090:intron 25.0%
!! TAATTTGCCTTAAGTGAATT+TGG - chr1_1:22143628-22143647 None:intergenic 25.0%
!!! GTTACTGAGATTTTTTTCAT+AGG + chr1_1:22143660-22143679 Msa0014090:intron 25.0%
! TTGAAACTTTGCATACAATA+TGG + chr1_1:22143729-22143748 Msa0014090:intron 25.0%
!!! GGATGATTCTGTTTTTTTTA+AGG + chr1_1:22143750-22143769 Msa0014090:intron 25.0%
!! ACATATATACTTCTCTCTTT+TGG + chr1_1:22143925-22143944 Msa0014090:intron 25.0%
!! TTAGTTGATATAGAGAATGT+TGG + chr1_1:22144291-22144310 Msa0014090:intron 25.0%
! GTTTATGTTAAAGTTAGGTT+AGG + chr1_1:22144424-22144443 Msa0014090:intron 25.0%
!! TATACGAGTGTTTTATAAGA+AGG + chr1_1:22144552-22144571 Msa0014090:CDS 25.0%
!! ATACGAGTGTTTTATAAGAA+GGG + chr1_1:22144553-22144572 Msa0014090:CDS 25.0%
! AAAATGCTGACAAGTATTTA+AGG + chr1_1:22143090-22143109 Msa0014090:CDS 25.0%
!!! TACTAAGTTTTAAGCAACTT+CGG + chr1_1:22143296-22143315 Msa0014090:intron 25.0%
! CATTTGATTGAATTAAACCT+TGG + chr1_1:22143560-22143579 Msa0014090:intron 25.0%
! TCATTATCCAAATTCACTTA+AGG + chr1_1:22143618-22143637 Msa0014090:intron 25.0%
!! TAATTTGCCTTAAGTGAATT+TGG - chr1_1:22143628-22143647 None:intergenic 25.0%
!!! GTTACTGAGATTTTTTTCAT+AGG + chr1_1:22143660-22143679 Msa0014090:intron 25.0%
! TTGAAACTTTGCATACAATA+TGG + chr1_1:22143729-22143748 Msa0014090:intron 25.0%
!!! GGATGATTCTGTTTTTTTTA+AGG + chr1_1:22143750-22143769 Msa0014090:intron 25.0%
!! ACATATATACTTCTCTCTTT+TGG + chr1_1:22143925-22143944 Msa0014090:intron 25.0%
!! TTAGTTGATATAGAGAATGT+TGG + chr1_1:22144291-22144310 Msa0014090:intron 25.0%
! GTTTATGTTAAAGTTAGGTT+AGG + chr1_1:22144424-22144443 Msa0014090:intron 25.0%
!! TATACGAGTGTTTTATAAGA+AGG + chr1_1:22144552-22144571 Msa0014090:CDS 25.0%
!! ATACGAGTGTTTTATAAGAA+GGG + chr1_1:22144553-22144572 Msa0014090:CDS 25.0%
! CATTTTCAACCTTTCCAAAA+CGG - chr1_1:22143076-22143095 None:intergenic 30.0%
GTCTCATATATACTCACATT+TGG - chr1_1:22143140-22143159 None:intergenic 30.0%
!!! TATCATCTGGCTTTGTATTT+TGG + chr1_1:22143342-22143361 Msa0014090:intron 30.0%
! TTTGCTTTGTTCAAATTCGT+AGG + chr1_1:22143433-22143452 Msa0014090:intron 30.0%
! TTGCTTTGTTCAAATTCGTA+GGG + chr1_1:22143434-22143453 Msa0014090:intron 30.0%
ATTAGTAACAATTGTGAGTC+CGG + chr1_1:22143503-22143522 Msa0014090:intron 30.0%
AAACAAAGAAGAACAAAACC+CGG - chr1_1:22143525-22143544 None:intergenic 30.0%
!! TGAAAAAACAAAGTGCTGTA+TGG + chr1_1:22143688-22143707 Msa0014090:intron 30.0%
! ACTTCTCTCTTTTGGATAAA+AGG + chr1_1:22143933-22143952 Msa0014090:intron 30.0%
! ATGTTAGTTATGGTTTTCAG+TGG + chr1_1:22143980-22143999 Msa0014090:intron 30.0%
GATATAGAGAATGTTGGAAA+AGG + chr1_1:22144297-22144316 Msa0014090:intron 30.0%
TTGTTGCAGAAGAATTTGAT+TGG + chr1_1:22144376-22144395 Msa0014090:CDS 30.0%
! GAAGAATTTGATTGGTGATT+CGG + chr1_1:22144384-22144403 Msa0014090:CDS 30.0%
ATAAAACACTCGTATAAACG+AGG - chr1_1:22144548-22144567 None:intergenic 30.0%
! CATTTTCAACCTTTCCAAAA+CGG - chr1_1:22143076-22143095 None:intergenic 30.0%
GTCTCATATATACTCACATT+TGG - chr1_1:22143140-22143159 None:intergenic 30.0%
!!! TATCATCTGGCTTTGTATTT+TGG + chr1_1:22143342-22143361 Msa0014090:intron 30.0%
! TTTGCTTTGTTCAAATTCGT+AGG + chr1_1:22143433-22143452 Msa0014090:intron 30.0%
! TTGCTTTGTTCAAATTCGTA+GGG + chr1_1:22143434-22143453 Msa0014090:intron 30.0%
ATTAGTAACAATTGTGAGTC+CGG + chr1_1:22143503-22143522 Msa0014090:intron 30.0%
AAACAAAGAAGAACAAAACC+CGG - chr1_1:22143525-22143544 None:intergenic 30.0%
!! TGAAAAAACAAAGTGCTGTA+TGG + chr1_1:22143688-22143707 Msa0014090:intron 30.0%
! ACTTCTCTCTTTTGGATAAA+AGG + chr1_1:22143933-22143952 Msa0014090:intron 30.0%
! ATGTTAGTTATGGTTTTCAG+TGG + chr1_1:22143980-22143999 Msa0014090:intron 30.0%
GATATAGAGAATGTTGGAAA+AGG + chr1_1:22144297-22144316 Msa0014090:intron 30.0%
TTGTTGCAGAAGAATTTGAT+TGG + chr1_1:22144376-22144395 Msa0014090:CDS 30.0%
! GAAGAATTTGATTGGTGATT+CGG + chr1_1:22144384-22144403 Msa0014090:CDS 30.0%
ATAAAACACTCGTATAAACG+AGG - chr1_1:22144548-22144567 None:intergenic 30.0%
ATATATGAGACATTGATCGC+TGG + chr1_1:22143149-22143168 Msa0014090:CDS 35.0%
TCTTCAGCTTCACAATGAAT+TGG + chr1_1:22143202-22143221 Msa0014090:CDS 35.0%
!!! TAACATGTGCTGCTTTTGAA+AGG - chr1_1:22143258-22143277 None:intergenic 35.0%
!! GATGATAAACGTGAGTTGAT+GGG - chr1_1:22143329-22143348 None:intergenic 35.0%
! AGATGATAAACGTGAGTTGA+TGG - chr1_1:22143330-22143349 None:intergenic 35.0%
ATCAACTCACGTTTATCATC+TGG + chr1_1:22143329-22143348 Msa0014090:intron 35.0%
TTAGTAACAATTGTGAGTCC+GGG + chr1_1:22143504-22143523 Msa0014090:intron 35.0%
GTCTTCACAAATCAATTCAG+AGG + chr1_1:22143813-22143832 Msa0014090:intron 35.0%
TGGAATTCATTGTCTTCGTT+TGG + chr1_1:22144000-22144019 Msa0014090:intron 35.0%
TAAAGTTAGGTTAGGCGATT+TGG + chr1_1:22144432-22144451 Msa0014090:intron 35.0%
! TGATGTTGAGAAGAGGTTTT+AGG + chr1_1:22144503-22144522 Msa0014090:CDS 35.0%
! GATGTTGAGAAGAGGTTTTA+GGG + chr1_1:22144504-22144523 Msa0014090:CDS 35.0%
ATATATGAGACATTGATCGC+TGG + chr1_1:22143149-22143168 Msa0014090:CDS 35.0%
TCTTCAGCTTCACAATGAAT+TGG + chr1_1:22143202-22143221 Msa0014090:CDS 35.0%
!!! TAACATGTGCTGCTTTTGAA+AGG - chr1_1:22143258-22143277 None:intergenic 35.0%
!! GATGATAAACGTGAGTTGAT+GGG - chr1_1:22143329-22143348 None:intergenic 35.0%
! AGATGATAAACGTGAGTTGA+TGG - chr1_1:22143330-22143349 None:intergenic 35.0%
ATCAACTCACGTTTATCATC+TGG + chr1_1:22143329-22143348 Msa0014090:intron 35.0%
TTAGTAACAATTGTGAGTCC+GGG + chr1_1:22143504-22143523 Msa0014090:intron 35.0%
GTCTTCACAAATCAATTCAG+AGG + chr1_1:22143813-22143832 Msa0014090:intron 35.0%
TGGAATTCATTGTCTTCGTT+TGG + chr1_1:22144000-22144019 Msa0014090:intron 35.0%
TAAAGTTAGGTTAGGCGATT+TGG + chr1_1:22144432-22144451 Msa0014090:intron 35.0%
! TGATGTTGAGAAGAGGTTTT+AGG + chr1_1:22144503-22144522 Msa0014090:CDS 35.0%
! GATGTTGAGAAGAGGTTTTA+GGG + chr1_1:22144504-22144523 Msa0014090:CDS 35.0%
!!! ATTCAGTGTTTGTGCCGTTT+TGG + chr1_1:22143059-22143078 Msa0014090:CDS 40.0%
TTTCATCATTTCCCTTCTGC+AGG - chr1_1:22143178-22143197 None:intergenic 40.0%
TGCAGAAGGGAAATGATGAA+AGG + chr1_1:22143177-22143196 Msa0014090:CDS 40.0%
GCAGAAGGGAAATGATGAAA+GGG + chr1_1:22143178-22143197 Msa0014090:CDS 40.0%
! TCACAATGAATTGGCCTCAT+TGG + chr1_1:22143211-22143230 Msa0014090:CDS 40.0%
ACTATGTTGCAACTCCAATG+AGG - chr1_1:22143228-22143247 None:intergenic 40.0%
!!! CATCTGGCTTTGTATTTTGG+AGG + chr1_1:22143345-22143364 Msa0014090:intron 40.0%
TGATCATAACAGTGCAACCA+AGG - chr1_1:22143580-22143599 None:intergenic 40.0%
ATAGCACAGAGTCATATCAG+TGG + chr1_1:22143889-22143908 Msa0014090:intron 40.0%
! AGATGCGCGTTTTACACTAT+TGG - chr1_1:22144106-22144125 None:intergenic 40.0%
! TTGAAAACAGTGCGTTTTGG+TGG - chr1_1:22144190-22144209 None:intergenic 40.0%
! GTGTTGAAAACAGTGCGTTT+TGG - chr1_1:22144193-22144212 None:intergenic 40.0%
AACACTTCTCAGTTTCTCGA+TGG + chr1_1:22144267-22144286 Msa0014090:intron 40.0%
ACACTTCTCAGTTTCTCGAT+GGG + chr1_1:22144268-22144287 Msa0014090:intron 40.0%
GAGAATGTTGGAAAAGGAAG+AGG + chr1_1:22144303-22144322 Msa0014090:intron 40.0%
GAAGAGGAAAAAGACGATGA+AGG + chr1_1:22144319-22144338 Msa0014090:intron 40.0%
!! AGGCGATTTGGATTTTGCTT+TGG + chr1_1:22144444-22144463 Msa0014090:CDS 40.0%
TTGGAGATGATGTTGAGAAG+AGG + chr1_1:22144496-22144515 Msa0014090:CDS 40.0%
!!! ATTCAGTGTTTGTGCCGTTT+TGG + chr1_1:22143059-22143078 Msa0014090:CDS 40.0%
TTTCATCATTTCCCTTCTGC+AGG - chr1_1:22143178-22143197 None:intergenic 40.0%
TGCAGAAGGGAAATGATGAA+AGG + chr1_1:22143177-22143196 Msa0014090:CDS 40.0%
GCAGAAGGGAAATGATGAAA+GGG + chr1_1:22143178-22143197 Msa0014090:CDS 40.0%
! TCACAATGAATTGGCCTCAT+TGG + chr1_1:22143211-22143230 Msa0014090:CDS 40.0%
ACTATGTTGCAACTCCAATG+AGG - chr1_1:22143228-22143247 None:intergenic 40.0%
!!! CATCTGGCTTTGTATTTTGG+AGG + chr1_1:22143345-22143364 Msa0014090:intron 40.0%
TGATCATAACAGTGCAACCA+AGG - chr1_1:22143580-22143599 None:intergenic 40.0%
ATAGCACAGAGTCATATCAG+TGG + chr1_1:22143889-22143908 Msa0014090:intron 40.0%
! AGATGCGCGTTTTACACTAT+TGG - chr1_1:22144106-22144125 None:intergenic 40.0%
! TTGAAAACAGTGCGTTTTGG+TGG - chr1_1:22144190-22144209 None:intergenic 40.0%
! GTGTTGAAAACAGTGCGTTT+TGG - chr1_1:22144193-22144212 None:intergenic 40.0%
AACACTTCTCAGTTTCTCGA+TGG + chr1_1:22144267-22144286 Msa0014090:intron 40.0%
ACACTTCTCAGTTTCTCGAT+GGG + chr1_1:22144268-22144287 Msa0014090:intron 40.0%
GAGAATGTTGGAAAAGGAAG+AGG + chr1_1:22144303-22144322 Msa0014090:intron 40.0%
GAAGAGGAAAAAGACGATGA+AGG + chr1_1:22144319-22144338 Msa0014090:intron 40.0%
!! AGGCGATTTGGATTTTGCTT+TGG + chr1_1:22144444-22144463 Msa0014090:CDS 40.0%
TTGGAGATGATGTTGAGAAG+AGG + chr1_1:22144496-22144515 Msa0014090:CDS 40.0%
!!! GTGTTTGTGCCGTTTTGGAA+AGG + chr1_1:22143064-22143083 Msa0014090:CDS 45.0%
ACTTCACTCACATACAAGCG+CGG + chr1_1:22144227-22144246 Msa0014090:intron 45.0%
!!! GTGTTTGTGCCGTTTTGGAA+AGG + chr1_1:22143064-22143083 Msa0014090:CDS 45.0%
ACTTCACTCACATACAAGCG+CGG + chr1_1:22144227-22144246 Msa0014090:intron 45.0%
ATCGCTGGTCACCTGCAGAA+GGG + chr1_1:22143164-22143183 Msa0014090:CDS 55.0%
ATCGCTGGTCACCTGCAGAA+GGG + chr1_1:22143164-22143183 Msa0014090:CDS 55.0%
GATCGCTGGTCACCTGCAGA+AGG + chr1_1:22143163-22143182 Msa0014090:CDS 60.0%
GATCGCTGGTCACCTGCAGA+AGG + chr1_1:22143163-22143182 Msa0014090:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 22143056 22144582 22143056 ID=Msa0014090;Name=Msa0014090
chr1_1 mRNA 22143056 22144582 22143056 ID=Msa0014090-mRNA-1;Parent=Msa0014090;Name=Msa0014090-mRNA-1;_AED=0.43;_eAED=0.50;_QI=0|0|0|1|0|0.25|4|0|146
chr1_1 exon 22143056 22143245 22143056 ID=Msa0014090-mRNA-1:exon:7775;Parent=Msa0014090-mRNA-1
chr1_1 exon 22144323 22144435 22144323 ID=Msa0014090-mRNA-1:exon:7776;Parent=Msa0014090-mRNA-1
chr1_1 exon 22144441 22144488 22144441 ID=Msa0014090-mRNA-1:exon:7777;Parent=Msa0014090-mRNA-1
chr1_1 exon 22144493 22144582 22144493 ID=Msa0014090-mRNA-1:exon:7778;Parent=Msa0014090-mRNA-1
chr1_1 CDS 22143056 22143245 22143056 ID=Msa0014090-mRNA-1:cds;Parent=Msa0014090-mRNA-1
chr1_1 CDS 22144323 22144435 22144323 ID=Msa0014090-mRNA-1:cds;Parent=Msa0014090-mRNA-1
chr1_1 CDS 22144441 22144488 22144441 ID=Msa0014090-mRNA-1:cds;Parent=Msa0014090-mRNA-1
chr1_1 CDS 22144493 22144582 22144493 ID=Msa0014090-mRNA-1:cds;Parent=Msa0014090-mRNA-1
Gene Sequence

>Msa0014090

ATGATTCAGTGTTTGTGCCGTTTTGGAAAGGTTGAAAATGCTGACAAGTATTTAAGGACTATAAAAGGTCGATTGCTGTTTCCAAATGTGAGTATATATGAGACATTGATCGCTGGTCACCTGCAGAAGGGAAATGATGAAAGGGCTCTTCAGCTTCACAATGAATTGGCCTCATTGGAGTTGCAACATAAGGAAAAAGACGATGAAGGTGAGTTAGTTAAGTTAGTTACATTGTTGAAGAGATTGTTGCAGAAGAATTTGATTGGTGATTCGGTTCCTTATTTGTTGATTGTTTATGTTAAAGTTAGGCGATTTGGATTTTGCTTTGGAGATGTATACATTATTTTTTTGAAATTGGAGATGATGTTGAGAAGAGGTTTTAGGGAGAATTTGTTTGTTAACACCTCGTTTATACGAGTGTTTTATAAGAAGGGACATTGA

Protein sequence

>Msa0014090

MIQCLCRFGKVENADKYLRTIKGRLLFPNVSIYETLIAGHLQKGNDERALQLHNELASLELQHKEKDDEGELVKLVTLLKRLLQKNLIGDSVPYLLIVYVKVRRFGFCFGDVYIIFLKLEMMLRRGFRENLFVNTSFIRVFYKKGH*