AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0907260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0907260 MtrunA17_Chr1g0204731 89.855 69 7 0 4 72 67 135 1.68e-38 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0907260 AT1G04200.1 46.479 71 33 1 4 69 67 137 1.26e-11 59.3
Msa0907260 AT1G04200.3 46.479 71 33 1 4 69 67 137 1.39e-11 59.3
Msa0907260 AT1G04200.2 46.479 71 33 1 4 69 67 137 1.42e-11 59.3

Find 15 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCTTATCAAGTATTTAT+TGG 0.232767 6_2:+5848066 Msa0907260:CDS
GGAACTCTATTATAGTATTT+AGG 0.245094 6_2:+5848111 Msa0907260:CDS
TATCTTATCAAGTATTTATT+GGG 0.279015 6_2:+5848067 Msa0907260:CDS
ACATCTAGCATGCTACTTGC+AGG 0.396925 6_2:+5847803 Msa0907260:CDS
TACTACTGGTGTGCCGCCCT+TGG 0.436969 6_2:+5847836 Msa0907260:CDS
CAATTGTATCTATCTTTCAA+TGG 0.466749 6_2:+5847930 Msa0907260:CDS
CAGGAGTCCATGTCTACTAC+TGG 0.477679 6_2:+5847822 Msa0907260:CDS
TTGAAGCACTTGATTGAAAG+TGG 0.483841 6_2:+5847903 Msa0907260:CDS
ATCTCAGTATAAGAAAGTTA+AGG 0.499204 6_2:+5848090 Msa0907260:CDS
GCCGCCCTTGGTTTATGAAA+AGG 0.503373 6_2:+5847848 Msa0907260:CDS
CACAAGCTTTGCAAAATGCC+TGG 0.519018 6_2:-5847778 None:intergenic
CGGCACACCAGTAGTAGACA+TGG 0.592941 6_2:-5847829 None:intergenic
TGGTGATGAAGTTGTACTGA+AGG 0.628038 6_2:+5847950 Msa0907260:CDS
GCTGCTAATGCAGTGTACAT+CGG 0.647239 6_2:+5847870 Msa0907260:CDS
AAAACATTAATTGTCACACC+AGG 0.713189 6_2:+5847760 Msa0907260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTATCAAGTATTTATT+GGG + chr6_2:5848067-5848086 Msa0907260:CDS 15.0%
!! CTATCTTATCAAGTATTTAT+TGG + chr6_2:5848066-5848085 Msa0907260:CDS 20.0%
! CAATTGTATCTATCTTTCAA+TGG + chr6_2:5847930-5847949 Msa0907260:CDS 25.0%
! ATCTCAGTATAAGAAAGTTA+AGG + chr6_2:5848090-5848109 Msa0907260:CDS 25.0%
AAAACATTAATTGTCACACC+AGG + chr6_2:5847760-5847779 Msa0907260:CDS 30.0%
! TTGTACTGAAGGATGTTTTA+GGG + chr6_2:5847961-5847980 Msa0907260:intron 30.0%
! GAAGTTAGAACCATGAATTT+TGG + chr6_2:5848034-5848053 Msa0907260:intron 30.0%
GTTCTAATCTCCAAAATTCA+TGG - chr6_2:5848047-5848066 None:intergenic 30.0%
! TTGAAGCACTTGATTGAAAG+TGG + chr6_2:5847903-5847922 Msa0907260:CDS 35.0%
! GTTGTACTGAAGGATGTTTT+AGG + chr6_2:5847960-5847979 Msa0907260:intron 35.0%
!! TGTACTGAAGGATGTTTTAG+GGG + chr6_2:5847962-5847981 Msa0907260:intron 35.0%
! CGCGTGTTAGATTTTACTTT+CGG + chr6_2:5847998-5848017 Msa0907260:intron 35.0%
! GCGTGTTAGATTTTACTTTC+GGG + chr6_2:5847999-5848018 Msa0907260:intron 35.0%
! GCAGCCTTTTCATAAACCAA+GGG - chr6_2:5847855-5847874 None:intergenic 40.0%
! AGCAGCCTTTTCATAAACCA+AGG - chr6_2:5847856-5847875 None:intergenic 40.0%
! TGGTGATGAAGTTGTACTGA+AGG + chr6_2:5847950-5847969 Msa0907260:CDS 40.0%
!! GTACTGAAGGATGTTTTAGG+GGG + chr6_2:5847963-5847982 Msa0907260:intron 40.0%
CACAAGCTTTGCAAAATGCC+TGG - chr6_2:5847781-5847800 None:intergenic 45.0%
! ACATCTAGCATGCTACTTGC+AGG + chr6_2:5847803-5847822 Msa0907260:CDS 45.0%
! GCCTTTTCATAAACCAAGGG+CGG - chr6_2:5847852-5847871 None:intergenic 45.0%
GCTGCTAATGCAGTGTACAT+CGG + chr6_2:5847870-5847889 Msa0907260:CDS 45.0%
CAGGAGTCCATGTCTACTAC+TGG + chr6_2:5847822-5847841 Msa0907260:CDS 50.0%
GCCGCCCTTGGTTTATGAAA+AGG + chr6_2:5847848-5847867 Msa0907260:CDS 50.0%
CGGCACACCAGTAGTAGACA+TGG - chr6_2:5847832-5847851 None:intergenic 55.0%
TACTACTGGTGTGCCGCCCT+TGG + chr6_2:5847836-5847855 Msa0907260:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 5847759 5848132 5847759 ID=Msa0907260;Name=Msa0907260
chr6_2 mRNA 5847759 5848132 5847759 ID=Msa0907260-mRNA-1;Parent=Msa0907260;Name=Msa0907260-mRNA-1;_AED=0.28;_eAED=0.27;_QI=0|0|0|1|0|0|2|0|94
chr6_2 exon 5847759 5847974 5847759 ID=Msa0907260-mRNA-1:exon:1833;Parent=Msa0907260-mRNA-1
chr6_2 exon 5848064 5848132 5848064 ID=Msa0907260-mRNA-1:exon:1834;Parent=Msa0907260-mRNA-1
chr6_2 CDS 5847759 5847974 5847759 ID=Msa0907260-mRNA-1:cds;Parent=Msa0907260-mRNA-1
chr6_2 CDS 5848064 5848132 5848064 ID=Msa0907260-mRNA-1:cds;Parent=Msa0907260-mRNA-1
Gene Sequence

>Msa0907260

AAAAACATTAATTGTCACACCAGGCATTTTGCAAAGCTTGTGTTACATCTAGCATGCTACTTGCAGGAGTCCATGTCTACTACTGGTGTGCCGCCCTTGGTTTATGAAAAGGCTGCTAATGCAGTGTACATCGGTTCTGTGTTTTTGAAGCACTTGATTGAAAGTGGTATGCAATTGTATCTATCTTTCAATGGTGATGAAGTTGTACTGAAGGATAACTATCTTATCAAGTATTTATTGGGATCTCAGTATAAGAAAGTTAAGGAACTCTATTATAGTATTTAG

Protein sequence

>Msa0907260

KNINCHTRHFAKLVLHLACYLQESMSTTGVPPLVYEKAANAVYIGSVFLKHLIESGMQLYLSFNGDEVVLKDNYLIKYLLGSQYKKVKELYYSI*