AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0956270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0956270 MtrunA17_Chr1g0204731 89.855 69 7 0 4 72 67 135 1.68e-38 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0956270 AT1G04200.1 46.479 71 33 1 4 69 67 137 1.26e-11 59.3
Msa0956270 AT1G04200.3 46.479 71 33 1 4 69 67 137 1.39e-11 59.3
Msa0956270 AT1G04200.2 46.479 71 33 1 4 69 67 137 1.42e-11 59.3

Find 15 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCTTATCAAGTATTTAT+TGG 0.232767 6_3:+62817363 Msa0956270:CDS
GGAACTCTATTATAGTATTT+AGG 0.245094 6_3:+62817408 Msa0956270:CDS
TATCTTATCAAGTATTTATT+GGG 0.279015 6_3:+62817364 Msa0956270:CDS
ACATCTAGCATGCTACTTGC+AGG 0.396925 6_3:+62817100 Msa0956270:CDS
TACTACTGGTGTGCCGCCCT+TGG 0.436969 6_3:+62817133 Msa0956270:CDS
CAATTGTATCTATCTTTCAA+TGG 0.466749 6_3:+62817227 Msa0956270:CDS
CAGGAGTCCATGTCTACTAC+TGG 0.477679 6_3:+62817119 Msa0956270:CDS
TTGAAGCACTTGATTGAAAG+TGG 0.483841 6_3:+62817200 Msa0956270:CDS
ATCTCAGTATAAGAAAGTTA+AGG 0.499204 6_3:+62817387 Msa0956270:CDS
GCCGCCCTTGGTTTATGAAA+AGG 0.503373 6_3:+62817145 Msa0956270:CDS
CACAAGCTTTGCAAAATGCC+TGG 0.519018 6_3:-62817075 None:intergenic
CGGCACACCAGTAGTAGACA+TGG 0.592941 6_3:-62817126 None:intergenic
TGGTGATGAAGTTGTACTGA+AGG 0.628038 6_3:+62817247 Msa0956270:CDS
GCTGCTAATGCAGTGTACAT+CGG 0.647239 6_3:+62817167 Msa0956270:CDS
AAAACATTAATTGTCACACC+AGG 0.713189 6_3:+62817057 Msa0956270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTATCAAGTATTTATT+GGG + chr6_3:62817364-62817383 Msa0956270:CDS 15.0%
!! CTATCTTATCAAGTATTTAT+TGG + chr6_3:62817363-62817382 Msa0956270:CDS 20.0%
! CAATTGTATCTATCTTTCAA+TGG + chr6_3:62817227-62817246 Msa0956270:CDS 25.0%
! ATCTCAGTATAAGAAAGTTA+AGG + chr6_3:62817387-62817406 Msa0956270:CDS 25.0%
AAAACATTAATTGTCACACC+AGG + chr6_3:62817057-62817076 Msa0956270:CDS 30.0%
! TTGTACTGAAGGATGTTTTA+GGG + chr6_3:62817258-62817277 Msa0956270:intron 30.0%
! GAAGTTAGAACCATGAATTT+TGG + chr6_3:62817331-62817350 Msa0956270:intron 30.0%
GTTCTAATCTCCAAAATTCA+TGG - chr6_3:62817344-62817363 None:intergenic 30.0%
! TTGAAGCACTTGATTGAAAG+TGG + chr6_3:62817200-62817219 Msa0956270:CDS 35.0%
! GTTGTACTGAAGGATGTTTT+AGG + chr6_3:62817257-62817276 Msa0956270:intron 35.0%
!! TGTACTGAAGGATGTTTTAG+GGG + chr6_3:62817259-62817278 Msa0956270:intron 35.0%
! CGCGTGTTAGATTTTACTTT+CGG + chr6_3:62817295-62817314 Msa0956270:intron 35.0%
! GCGTGTTAGATTTTACTTTC+GGG + chr6_3:62817296-62817315 Msa0956270:intron 35.0%
! GCAGCCTTTTCATAAACCAA+GGG - chr6_3:62817152-62817171 None:intergenic 40.0%
! AGCAGCCTTTTCATAAACCA+AGG - chr6_3:62817153-62817172 None:intergenic 40.0%
! TGGTGATGAAGTTGTACTGA+AGG + chr6_3:62817247-62817266 Msa0956270:CDS 40.0%
!! GTACTGAAGGATGTTTTAGG+GGG + chr6_3:62817260-62817279 Msa0956270:intron 40.0%
CACAAGCTTTGCAAAATGCC+TGG - chr6_3:62817078-62817097 None:intergenic 45.0%
! ACATCTAGCATGCTACTTGC+AGG + chr6_3:62817100-62817119 Msa0956270:CDS 45.0%
! GCCTTTTCATAAACCAAGGG+CGG - chr6_3:62817149-62817168 None:intergenic 45.0%
GCTGCTAATGCAGTGTACAT+CGG + chr6_3:62817167-62817186 Msa0956270:CDS 45.0%
CAGGAGTCCATGTCTACTAC+TGG + chr6_3:62817119-62817138 Msa0956270:CDS 50.0%
GCCGCCCTTGGTTTATGAAA+AGG + chr6_3:62817145-62817164 Msa0956270:CDS 50.0%
CGGCACACCAGTAGTAGACA+TGG - chr6_3:62817129-62817148 None:intergenic 55.0%
TACTACTGGTGTGCCGCCCT+TGG + chr6_3:62817133-62817152 Msa0956270:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 62817056 62817429 62817056 ID=Msa0956270;Name=Msa0956270
chr6_3 mRNA 62817056 62817429 62817056 ID=Msa0956270-mRNA-1;Parent=Msa0956270;Name=Msa0956270-mRNA-1;_AED=0.28;_eAED=0.27;_QI=0|0|0|1|0|0|2|0|94
chr6_3 exon 62817056 62817271 62817056 ID=Msa0956270-mRNA-1:exon:12103;Parent=Msa0956270-mRNA-1
chr6_3 exon 62817361 62817429 62817361 ID=Msa0956270-mRNA-1:exon:12104;Parent=Msa0956270-mRNA-1
chr6_3 CDS 62817056 62817271 62817056 ID=Msa0956270-mRNA-1:cds;Parent=Msa0956270-mRNA-1
chr6_3 CDS 62817361 62817429 62817361 ID=Msa0956270-mRNA-1:cds;Parent=Msa0956270-mRNA-1
Gene Sequence

>Msa0956270

AAAAACATTAATTGTCACACCAGGCATTTTGCAAAGCTTGTGTTACATCTAGCATGCTACTTGCAGGAGTCCATGTCTACTACTGGTGTGCCGCCCTTGGTTTATGAAAAGGCTGCTAATGCAGTGTACATCGGTTCTGTGTTTTTGAAGCACTTGATTGAAAGTGGTATGCAATTGTATCTATCTTTCAATGGTGATGAAGTTGTACTGAAGGATAACTATCTTATCAAGTATTTATTGGGATCTCAGTATAAGAAAGTTAAGGAACTCTATTATAGTATTTAG

Protein sequence

>Msa0956270

KNINCHTRHFAKLVLHLACYLQESMSTTGVPPLVYEKAANAVYIGSVFLKHLIESGMQLYLSFNGDEVVLKDNYLIKYLLGSQYKKVKELYYSI*