AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0016330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016330 MtrunA17_Chr1g0196771 96.018 226 9 0 1 226 1 226 3.71e-165 454
Msa0016330 MtrunA17_Chr1g0212301 49.321 221 108 2 1 218 1 220 4.48e-75 226
Msa0016330 MtrunA17_Chr4g0008781 45.755 212 112 2 1 211 6 215 6.29e-68 208
Msa0016330 MtrunA17_Chr1g0196661 50.000 212 103 2 1 210 1 211 8.58e-68 207
Msa0016330 MtrunA17_Chr4g0029341 47.867 211 109 1 2 211 3 213 2.90e-67 206
Msa0016330 MtrunA17_Chr7g0240561 47.511 221 111 3 1 219 5 222 1.40e-66 204
Msa0016330 MtrunA17_Chr8g0379581 48.182 220 111 2 2 220 4 221 4.63e-66 203
Msa0016330 MtrunA17_Chr7g0240581 46.364 220 113 2 2 219 6 222 5.05e-66 203
Msa0016330 MtrunA17_Chr4g0008791 46.154 221 114 3 3 221 7 224 1.42e-65 202
Msa0016330 MtrunA17_Chr3g0132581 46.575 219 114 2 2 219 4 220 7.05e-65 200
Msa0016330 MtrunA17_Chr7g0240551 48.598 214 105 3 2 213 6 216 1.11e-64 200
Msa0016330 MtrunA17_Chr7g0240681 45.161 217 105 4 2 211 6 215 4.15e-64 198
Msa0016330 MtrunA17_Chr7g0240541 47.664 214 107 3 2 213 6 216 1.04e-63 197
Msa0016330 MtrunA17_Chr6g0483141 46.919 211 109 2 1 210 1 209 1.50e-63 197
Msa0016330 MtrunA17_Chr1g0196691 46.479 213 112 1 1 211 26 238 1.57e-63 197
Msa0016330 MtrunA17_Chr1g0196721 49.048 210 104 2 4 211 3 211 2.30e-63 196
Msa0016330 MtrunA17_Chr7g0240631 44.595 222 118 3 2 221 6 224 2.61e-63 196
Msa0016330 MtrunA17_Chr7g0240511 46.364 220 108 4 2 219 6 217 7.94e-62 192
Msa0016330 MtrunA17_Chr7g0240641 44.395 223 119 3 1 221 5 224 3.30e-61 191
Msa0016330 MtrunA17_Chr7g0240531 46.729 214 109 3 2 213 6 216 4.31e-60 188
Msa0016330 MtrunA17_Chr7g0240691 42.601 223 122 3 2 221 36 255 4.37e-60 189
Msa0016330 MtrunA17_Chr8g0379561 44.700 217 117 2 2 217 5 219 1.41e-59 187
Msa0016330 MtrunA17_Chr1g0196711 45.972 211 111 3 1 209 1 210 4.26e-59 186
Msa0016330 MtrunA17_Chr7g0240611 42.793 222 118 4 2 221 6 220 4.17e-56 178
Msa0016330 MtrunA17_Chr7g0240521 45.701 221 114 4 2 219 13 230 3.64e-55 176
Msa0016330 MtrunA17_Chr7g0240671 45.238 210 106 4 4 211 5 207 1.53e-54 174
Msa0016330 MtrunA17_Chr5g0417011 40.654 214 122 4 3 211 7 220 4.74e-51 165
Msa0016330 MtrunA17_Chr7g0240651 42.727 220 117 4 2 219 6 218 2.82e-49 160
Msa0016330 MtrunA17_Chr5g0415411 40.741 216 117 4 3 209 5 218 3.28e-46 153
Msa0016330 MtrunA17_Chr7g0240661 41.981 212 114 4 2 211 6 210 3.53e-46 152
Msa0016330 MtrunA17_Chr2g0312771 38.288 222 123 6 2 217 3 216 1.31e-45 151
Msa0016330 MtrunA17_Chr2g0312731 38.009 221 119 7 2 212 3 215 4.81e-45 149
Msa0016330 MtrunA17_Chr8g0363751 38.961 231 130 5 3 224 5 233 5.50e-45 150
Msa0016330 MtrunA17_Chr2g0312721 37.209 215 129 4 2 214 3 213 6.23e-45 149
Msa0016330 MtrunA17_Chr2g0312751 37.387 222 125 6 2 217 3 216 1.56e-44 148
Msa0016330 MtrunA17_Chr2g0312811 35.909 220 133 5 2 217 3 218 2.44e-42 142
Msa0016330 MtrunA17_Chr2g0312781 38.426 216 123 5 2 211 20 231 2.79e-42 143
Msa0016330 MtrunA17_Chr3g0107731 40.000 210 111 7 3 205 9 210 1.18e-41 141
Msa0016330 MtrunA17_Chr2g0312791 37.379 206 122 5 2 204 3 204 7.13e-41 139
Msa0016330 MtrunA17_Chr3g0107741 40.094 212 110 8 2 205 8 210 8.92e-40 136
Msa0016330 MtrunA17_Chr2g0312681 36.842 209 126 4 3 209 4 208 5.63e-38 131
Msa0016330 MtrunA17_Chr8g0357241 36.493 211 126 5 10 217 11 216 2.35e-37 130
Msa0016330 MtrunA17_Chr5g0433801 37.156 218 129 6 2 216 5 217 6.11e-37 129
Msa0016330 MtrunA17_Chr8g0357211 45.333 150 76 4 10 156 11 157 7.66e-37 128
Msa0016330 MtrunA17_Chr4g0069471 46.584 161 78 6 1 156 22 179 9.92e-37 127
Msa0016330 MtrunA17_Chr8g0391631 43.949 157 81 5 1 150 19 175 3.77e-35 123
Msa0016330 MtrunA17_Chr2g0312691 37.900 219 122 6 3 215 4 214 1.72e-34 122
Msa0016330 MtrunA17_Chr2g0312711 36.652 221 123 5 3 216 4 214 3.75e-33 119
Msa0016330 MtrunA17_Chr2g0313561 46.552 58 30 1 47 103 2 59 1.46e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016330 AT2G29420.1 47.418 213 109 2 2 213 8 218 3.42e-66 204
Msa0016330 AT3G09270.1 47.887 213 106 4 2 211 5 215 1.91e-62 194
Msa0016330 AT2G29490.1 46.411 209 108 3 2 208 6 212 1.12e-57 182
Msa0016330 AT2G29460.1 45.070 213 113 3 2 212 6 216 4.36e-56 178
Msa0016330 AT2G29450.1 45.413 218 111 4 2 213 5 220 7.83e-56 177
Msa0016330 AT2G29480.1 45.070 213 113 3 2 212 6 216 1.45e-55 177
Msa0016330 AT2G29440.1 44.076 211 114 3 2 210 5 213 3.64e-52 168
Msa0016330 AT2G29470.1 42.453 212 117 4 2 210 6 215 1.63e-51 167
Msa0016330 AT5G62480.1 37.719 228 126 5 4 219 9 232 2.02e-50 164
Msa0016330 AT1G78320.1 37.104 221 133 4 2 220 3 219 5.61e-47 155
Msa0016330 AT1G74590.1 37.778 225 126 5 8 223 12 231 3.52e-46 153
Msa0016330 AT1G78370.1 39.904 208 119 4 10 215 11 214 5.38e-46 152
Msa0016330 AT1G27130.1 37.838 222 131 4 2 216 5 226 8.33e-46 152
Msa0016330 AT1G78380.1 40.367 218 124 4 3 218 4 217 1.87e-45 151
Msa0016330 AT1G17170.1 39.815 216 123 5 2 215 3 213 5.48e-45 150
Msa0016330 AT1G10370.1 39.815 216 124 3 3 212 5 220 2.25e-44 148
Msa0016330 AT1G78340.1 36.986 219 132 4 2 218 3 217 8.32e-43 144
Msa0016330 AT1G17180.1 37.037 216 130 4 2 215 3 214 1.66e-41 141
Msa0016330 AT3G43800.1 39.806 206 114 6 2 201 4 205 3.79e-40 137
Msa0016330 AT1G59670.1 38.991 218 126 5 2 212 5 222 1.88e-39 136
Msa0016330 AT1G59700.1 38.182 220 125 5 2 212 5 222 3.84e-39 135
Msa0016330 AT1G69930.1 35.814 215 133 3 2 211 11 225 1.40e-38 134
Msa0016330 AT1G27140.1 35.455 220 134 4 2 213 5 224 1.44e-38 134
Msa0016330 AT1G53680.1 36.239 218 131 4 4 217 8 221 5.78e-38 132
Msa0016330 AT1G17190.1 37.727 220 131 4 2 219 4 219 1.37e-37 130
Msa0016330 AT1G69920.1 36.652 221 131 5 4 216 35 254 2.29e-37 131
Msa0016330 AT1G10360.1 36.161 224 130 7 2 217 4 222 2.86e-36 127
Msa0016330 AT1G78360.1 36.199 221 128 6 3 217 4 217 1.68e-35 125
Msa0016330 AT5G62480.2 30.942 223 114 5 8 219 13 206 2.95e-33 119
Msa0016330 AT5G62480.3 30.942 223 113 5 8 219 13 205 7.13e-33 118
Msa0016330 AT1G78320.2 30.455 220 122 4 2 220 3 192 7.04e-30 110
Msa0016330 AT2G02390.1 26.636 214 133 5 2 201 7 210 5.06e-12 63.5
Msa0016330 AT2G02380.1 27.143 210 133 8 4 201 12 213 1.16e-11 62.4
Msa0016330 AT2G02390.3 25.339 221 134 5 2 201 7 217 2.40e-11 61.6

Find 58 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACAACCCTCTGAGTAAA+TGG 0.229034 1_1:+27138674 None:intergenic
AGAGGTCCAATGTTCGTTTC+TGG 0.238880 1_1:-27138445 Msa0016330:CDS
CATTTCCCCACCTTCACTCT+TGG 0.265735 1_1:-27137705 Msa0016330:CDS
CTAATGTTACGTACACTTTC+CGG 0.265881 1_1:-27137518 Msa0016330:three_prime_UTR
CTCTTGGGTCAAGAATTTCT+TGG 0.313611 1_1:-27137689 Msa0016330:CDS
ACAGGAACCTTCTTGTAGAT+TGG 0.343683 1_1:+27138569 None:intergenic
TTCATTGGTGGCAACACAAT+TGG 0.349905 1_1:-27137814 Msa0016330:CDS
ATAAATCATGGAGGATGTTA+AGG 0.353756 1_1:-27138717 None:intergenic
ATTTCCCCACCTTCACTCTT+GGG 0.369745 1_1:-27137704 Msa0016330:CDS
CACTAACTCATATTTCGCTT+TGG 0.370582 1_1:+27137353 None:intergenic
GTCAAAATTCATCGAGGAAA+AGG 0.403539 1_1:-27138423 Msa0016330:intron
GAGATCAATAAAAGAAACTT+TGG 0.416167 1_1:-27137884 Msa0016330:CDS
TTTCCGTTATGTACAAGAAC+AGG 0.426569 1_1:+27138551 None:intergenic
CACATATATCATTTAATGAA+TGG 0.430909 1_1:-27137424 Msa0016330:three_prime_UTR
GCTACTTGTTGAGTACTTCA+AGG 0.437991 1_1:-27137629 Msa0016330:CDS
TAAGCATCTTGTGGCAATAG+AGG 0.455670 1_1:+27138470 None:intergenic
GGATTGGCAGACATAGCCTT+AGG 0.466349 1_1:-27137793 Msa0016330:CDS
ATCACCATTGACTCTGCCAT+TGG 0.471524 1_1:+27138527 None:intergenic
GTGTGGACATTAAAAGTAAA+GGG 0.473984 1_1:-27138656 Msa0016330:CDS
TGTGTGGACATTAAAAGTAA+AGG 0.486415 1_1:-27138657 Msa0016330:CDS
CCAGTCATGGAAGAAATTGT+TGG 0.492761 1_1:-27137751 Msa0016330:CDS
TGGCAGACATAGCCTTAGGT+TGG 0.497374 1_1:-27137789 Msa0016330:CDS
AACTTTGAGAGTGATTGAAA+AGG 0.504867 1_1:-27137860 Msa0016330:CDS
AAAACCAATGGCAGAGTCAA+TGG 0.509729 1_1:-27138531 Msa0016330:CDS
AGAGTGATTGAAAAGGAAAG+TGG 0.513776 1_1:-27137853 Msa0016330:CDS
GTCAAGTCCATTTACTCAGA+GGG 0.514134 1_1:-27138681 Msa0016330:CDS
CTTAGTGAAAAGATGTTCAT+TGG 0.515605 1_1:-27137829 Msa0016330:CDS
CCAACAATTTCTTCCATGAC+TGG 0.525633 1_1:+27137751 None:intergenic
GTTCCTGTTCTTGTACATAA+CGG 0.527966 1_1:-27138554 Msa0016330:CDS
GTACTCAACAAGTAGCTCAT+GGG 0.541505 1_1:+27137636 None:intergenic
TGGTGGCAACACAATTGGAT+TGG 0.546011 1_1:-27137809 Msa0016330:CDS
TGCACATACTCTGCCAGTCA+TGG 0.554228 1_1:-27137764 Msa0016330:CDS
TCTTGACCCAAGAGTGAAGG+TGG 0.567830 1_1:+27137698 None:intergenic
CATTTACTCAGAGGGTTGTG+TGG 0.569240 1_1:-27138673 Msa0016330:CDS
TAACAATGACTGGAGAGTGC+AGG 0.569378 1_1:-27137377 Msa0016330:three_prime_UTR
AGTACTCAACAAGTAGCTCA+TGG 0.570685 1_1:+27137635 None:intergenic
AGTGAAAAGATGTTCATTGG+TGG 0.577298 1_1:-27137826 Msa0016330:CDS
TAAGTCGGTTGCTATGATCA+TGG 0.583468 1_1:+27137214 None:intergenic
TTGAATATATTGATGAGACT+TGG 0.584030 1_1:-27138502 Msa0016330:CDS
AAGCATCTTGTGGCAATAGA+GGG 0.589426 1_1:+27138471 None:intergenic
AGTATGTGCAATCCAACCTA+AGG 0.590838 1_1:+27137777 None:intergenic
GAGTGATTGAAAAGGAAAGT+GGG 0.591381 1_1:-27137852 Msa0016330:CDS
GACCTCTCATAAGCATCTTG+TGG 0.597852 1_1:+27138461 None:intergenic
TTGAGTACTTCAAGGAAAGG+AGG 0.598211 1_1:-27137621 Msa0016330:CDS
ATACAATCCAATCTACAAGA+AGG 0.599962 1_1:-27138576 Msa0016330:CDS
TTGTTGAGTACTTCAAGGAA+AGG 0.601650 1_1:-27137624 Msa0016330:CDS
AATTCTTGACCCAAGAGTGA+AGG 0.605291 1_1:+27137695 None:intergenic
TGTACATAACGGAAAACCAA+TGG 0.624083 1_1:-27138543 Msa0016330:CDS
CTTGACCCAAGAGTGAAGGT+GGG 0.630426 1_1:+27137699 None:intergenic
GGTCAAGTCCATTTACTCAG+AGG 0.633849 1_1:-27138682 Msa0016330:CDS
TACTCAACAAGTAGCTCATG+GGG 0.634231 1_1:+27137637 None:intergenic
GGTGTGAGTTATGAGTATTA+TGG 0.634625 1_1:-27138635 Msa0016330:CDS
GTACTTCAAGGAAAGGAGGA+AGG 0.655832 1_1:-27137617 Msa0016330:CDS
TGTGGCAATAGAGGGATCTG+AGG 0.656588 1_1:+27138479 None:intergenic
TCAACAAGTAGCTCATGGGG+TGG 0.666333 1_1:+27137640 None:intergenic
TGCCACAAGATGCTTATGAG+AGG 0.667321 1_1:-27138463 Msa0016330:CDS
TTGACCCAAGAGTGAAGGTG+GGG 0.669984 1_1:+27137700 None:intergenic
TTTCTGGTCAAAATTCATCG+AGG 0.698544 1_1:-27138429 Msa0016330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCGTATAAAATATTTTTT+TGG + chr1_1:27137741-27137760 None:intergenic 10.0%
!!! AATCGTATAAAATATTTTTT+TGG + chr1_1:27137741-27137760 None:intergenic 10.0%
!! TTTAAATTTGATTATGGAAA+GGG + chr1_1:27137520-27137539 None:intergenic 15.0%
!! AAATATAGAGAAAAAATCTA+CGG - chr1_1:27137810-27137829 Msa0016330:CDS 15.0%
!! AAAAGAACAAAAAATTCTAA+TGG + chr1_1:27138318-27138337 None:intergenic 15.0%
!!! CTTTTATGTATTAAATAACA+AGG - chr1_1:27138637-27138656 Msa0016330:CDS 15.0%
!! ATTCTCTATATATATATAGA+AGG - chr1_1:27138709-27138728 Msa0016330:CDS 15.0%
!! TTCTCTATATATATATAGAA+GGG - chr1_1:27138710-27138729 Msa0016330:CDS 15.0%
!! TTTAAATTTGATTATGGAAA+GGG + chr1_1:27137520-27137539 None:intergenic 15.0%
!! AAATATAGAGAAAAAATCTA+CGG - chr1_1:27137810-27137829 Msa0016330:CDS 15.0%
!! AAAAGAACAAAAAATTCTAA+TGG + chr1_1:27138318-27138337 None:intergenic 15.0%
!!! CTTTTATGTATTAAATAACA+AGG - chr1_1:27138637-27138656 Msa0016330:CDS 15.0%
!! ATTCTCTATATATATATAGA+AGG - chr1_1:27138709-27138728 Msa0016330:CDS 15.0%
!! GTTTAAATTTGATTATGGAA+AGG + chr1_1:27137521-27137540 None:intergenic 20.0%
!!! TTGTCGTTTAAATTTGATTA+TGG + chr1_1:27137526-27137545 None:intergenic 20.0%
!! TGATATTTAACCAGTAAATA+TGG - chr1_1:27137621-27137640 Msa0016330:CDS 20.0%
!! GATACAAAGATCTATTTAAA+AGG + chr1_1:27137672-27137691 None:intergenic 20.0%
!!! TTTTTGTTTATAATAGTGAC+CGG + chr1_1:27138385-27138404 None:intergenic 20.0%
!! CACATATATCATTTAATGAA+TGG - chr1_1:27138457-27138476 Msa0016330:CDS 20.0%
!!! TGTCTTTTTATTGTTTTTTC+AGG - chr1_1:27138549-27138568 Msa0016330:CDS 20.0%
!!! GTCTTTTTATTGTTTTTTCA+GGG - chr1_1:27138550-27138569 Msa0016330:CDS 20.0%
!! AACAAAAACTTAGATTAAGT+CGG + chr1_1:27138685-27138704 None:intergenic 20.0%
!! GTTTAAATTTGATTATGGAA+AGG + chr1_1:27137521-27137540 None:intergenic 20.0%
!!! TTGTCGTTTAAATTTGATTA+TGG + chr1_1:27137526-27137545 None:intergenic 20.0%
!! TGATATTTAACCAGTAAATA+TGG - chr1_1:27137621-27137640 Msa0016330:CDS 20.0%
!! GATACAAAGATCTATTTAAA+AGG + chr1_1:27137672-27137691 None:intergenic 20.0%
!!! TTTTTGTTTATAATAGTGAC+CGG + chr1_1:27138385-27138404 None:intergenic 20.0%
!! CACATATATCATTTAATGAA+TGG - chr1_1:27138457-27138476 Msa0016330:CDS 20.0%
!!! TGTCTTTTTATTGTTTTTTC+AGG - chr1_1:27138549-27138568 Msa0016330:CDS 20.0%
!!! GTCTTTTTATTGTTTTTTCA+GGG - chr1_1:27138550-27138569 Msa0016330:CDS 20.0%
!! AACAAAAACTTAGATTAAGT+CGG + chr1_1:27138685-27138704 None:intergenic 20.0%
! TTGAATATATTGATGAGACT+TGG - chr1_1:27137379-27137398 Msa0016330:three_prime_UTR 25.0%
!! TTACTGGTTAAATATCAAGT+TGG + chr1_1:27137618-27137637 None:intergenic 25.0%
! AAATGTTCTTCCATATTTAC+TGG + chr1_1:27137634-27137653 None:intergenic 25.0%
! TACGTTAACATTTGATATGT+TGG + chr1_1:27137854-27137873 None:intergenic 25.0%
! TCTCATAAGTATTACTTTGA+TGG + chr1_1:27137885-27137904 None:intergenic 25.0%
!! ATTTTATCCTACAATGACAT+TGG - chr1_1:27137909-27137928 Msa0016330:CDS 25.0%
! GAGATCAATAAAAGAAACTT+TGG - chr1_1:27137997-27138016 Msa0016330:intron 25.0%
!! TTTTTGCTTATAACAATGAC+TGG - chr1_1:27138494-27138513 Msa0016330:CDS 25.0%
! TTGAATATATTGATGAGACT+TGG - chr1_1:27137379-27137398 Msa0016330:three_prime_UTR 25.0%
!! TTACTGGTTAAATATCAAGT+TGG + chr1_1:27137618-27137637 None:intergenic 25.0%
! AAATGTTCTTCCATATTTAC+TGG + chr1_1:27137634-27137653 None:intergenic 25.0%
! TACGTTAACATTTGATATGT+TGG + chr1_1:27137854-27137873 None:intergenic 25.0%
! TCTCATAAGTATTACTTTGA+TGG + chr1_1:27137885-27137904 None:intergenic 25.0%
!! ATTTTATCCTACAATGACAT+TGG - chr1_1:27137909-27137928 Msa0016330:CDS 25.0%
! GAGATCAATAAAAGAAACTT+TGG - chr1_1:27137997-27138016 Msa0016330:intron 25.0%
!! TTTTTGCTTATAACAATGAC+TGG - chr1_1:27138494-27138513 Msa0016330:CDS 25.0%
!!! ATGTTAAGGTTTTTGGTCTA+TGG - chr1_1:27137178-27137197 Msa0016330:three_prime_UTR 30.0%
TGTGTGGACATTAAAAGTAA+AGG - chr1_1:27137224-27137243 Msa0016330:three_prime_UTR 30.0%
GTGTGGACATTAAAAGTAAA+GGG - chr1_1:27137225-27137244 Msa0016330:three_prime_UTR 30.0%
ATACAATCCAATCTACAAGA+AGG - chr1_1:27137305-27137324 Msa0016330:three_prime_UTR 30.0%
AATGTGACACAATTGACAAA+TGG - chr1_1:27137569-27137588 Msa0016330:exon 30.0%
AATCATTCCAATGTCATTGT+AGG + chr1_1:27137919-27137938 None:intergenic 30.0%
!!! ATGGAATTTTTTCTGTACGA+TGG - chr1_1:27137962-27137981 Msa0016330:intron 30.0%
! AACTTTGAGAGTGATTGAAA+AGG - chr1_1:27138021-27138040 Msa0016330:intron 30.0%
CTTAGTGAAAAGATGTTCAT+TGG - chr1_1:27138052-27138071 Msa0016330:intron 30.0%
!!! GGAAGATTGTTTTTTATAGC+AGG + chr1_1:27138223-27138242 None:intergenic 30.0%
!!! ATGTTAAGGTTTTTGGTCTA+TGG - chr1_1:27137178-27137197 Msa0016330:three_prime_UTR 30.0%
TGTGTGGACATTAAAAGTAA+AGG - chr1_1:27137224-27137243 Msa0016330:three_prime_UTR 30.0%
GTGTGGACATTAAAAGTAAA+GGG - chr1_1:27137225-27137244 Msa0016330:three_prime_UTR 30.0%
ATACAATCCAATCTACAAGA+AGG - chr1_1:27137305-27137324 Msa0016330:three_prime_UTR 30.0%
AATGTGACACAATTGACAAA+TGG - chr1_1:27137569-27137588 Msa0016330:exon 30.0%
AATCATTCCAATGTCATTGT+AGG + chr1_1:27137919-27137938 None:intergenic 30.0%
!!! ATGGAATTTTTTCTGTACGA+TGG - chr1_1:27137962-27137981 Msa0016330:intron 30.0%
! AACTTTGAGAGTGATTGAAA+AGG - chr1_1:27138021-27138040 Msa0016330:intron 30.0%
CTTAGTGAAAAGATGTTCAT+TGG - chr1_1:27138052-27138071 Msa0016330:intron 30.0%
!!! GGAAGATTGTTTTTTATAGC+AGG + chr1_1:27138223-27138242 None:intergenic 30.0%
! ATGGAGGATGTTAAGGTTTT+TGG - chr1_1:27137171-27137190 Msa0016330:three_prime_UTR 35.0%
GGTGTGAGTTATGAGTATTA+TGG - chr1_1:27137246-27137265 Msa0016330:three_prime_UTR 35.0%
GTTCCTGTTCTTGTACATAA+CGG - chr1_1:27137327-27137346 Msa0016330:three_prime_UTR 35.0%
TTTCCGTTATGTACAAGAAC+AGG + chr1_1:27137333-27137352 None:intergenic 35.0%
! TGTACATAACGGAAAACCAA+TGG - chr1_1:27137338-27137357 Msa0016330:three_prime_UTR 35.0%
! TTTTGACCAGAAACGAACAT+TGG + chr1_1:27137445-27137464 None:intergenic 35.0%
! TTTCTGGTCAAAATTCATCG+AGG - chr1_1:27137452-27137471 Msa0016330:three_prime_UTR 35.0%
GTCAAAATTCATCGAGGAAA+AGG - chr1_1:27137458-27137477 Msa0016330:three_prime_UTR 35.0%
TCAATTGTGTCACATTACGT+CGG + chr1_1:27137565-27137584 None:intergenic 35.0%
GGTCAAATATACCCTTCAAT+CGG + chr1_1:27137720-27137739 None:intergenic 35.0%
! TTTTCAGTCAATGCACATGA+TGG - chr1_1:27137943-27137962 Msa0016330:intron 35.0%
AGAGTGATTGAAAAGGAAAG+TGG - chr1_1:27138028-27138047 Msa0016330:intron 35.0%
GAGTGATTGAAAAGGAAAGT+GGG - chr1_1:27138029-27138048 Msa0016330:intron 35.0%
AGTGAAAAGATGTTCATTGG+TGG - chr1_1:27138055-27138074 Msa0016330:intron 35.0%
TTGTTGAGTACTTCAAGGAA+AGG - chr1_1:27138257-27138276 Msa0016330:intron 35.0%
CTAATGTTACGTACACTTTC+CGG - chr1_1:27138363-27138382 Msa0016330:intron 35.0%
CACTAACTCATATTTCGCTT+TGG + chr1_1:27138531-27138550 None:intergenic 35.0%
! ATGGAGGATGTTAAGGTTTT+TGG - chr1_1:27137171-27137190 Msa0016330:three_prime_UTR 35.0%
GGTGTGAGTTATGAGTATTA+TGG - chr1_1:27137246-27137265 Msa0016330:three_prime_UTR 35.0%
GTTCCTGTTCTTGTACATAA+CGG - chr1_1:27137327-27137346 Msa0016330:three_prime_UTR 35.0%
TTTCCGTTATGTACAAGAAC+AGG + chr1_1:27137333-27137352 None:intergenic 35.0%
! TGTACATAACGGAAAACCAA+TGG - chr1_1:27137338-27137357 Msa0016330:three_prime_UTR 35.0%
! TTTTGACCAGAAACGAACAT+TGG + chr1_1:27137445-27137464 None:intergenic 35.0%
! TTTCTGGTCAAAATTCATCG+AGG - chr1_1:27137452-27137471 Msa0016330:three_prime_UTR 35.0%
GTCAAAATTCATCGAGGAAA+AGG - chr1_1:27137458-27137477 Msa0016330:three_prime_UTR 35.0%
TCAATTGTGTCACATTACGT+CGG + chr1_1:27137565-27137584 None:intergenic 35.0%
GGTCAAATATACCCTTCAAT+CGG + chr1_1:27137720-27137739 None:intergenic 35.0%
! TTTTCAGTCAATGCACATGA+TGG - chr1_1:27137943-27137962 Msa0016330:intron 35.0%
AGAGTGATTGAAAAGGAAAG+TGG - chr1_1:27138028-27138047 Msa0016330:intron 35.0%
GAGTGATTGAAAAGGAAAGT+GGG - chr1_1:27138029-27138048 Msa0016330:intron 35.0%
AGTGAAAAGATGTTCATTGG+TGG - chr1_1:27138055-27138074 Msa0016330:intron 35.0%
TTGTTGAGTACTTCAAGGAA+AGG - chr1_1:27138257-27138276 Msa0016330:intron 35.0%
CTAATGTTACGTACACTTTC+CGG - chr1_1:27138363-27138382 Msa0016330:intron 35.0%
CACTAACTCATATTTCGCTT+TGG + chr1_1:27138531-27138550 None:intergenic 35.0%
GTCAAGTCCATTTACTCAGA+GGG - chr1_1:27137200-27137219 Msa0016330:three_prime_UTR 40.0%
ACAGGAACCTTCTTGTAGAT+TGG + chr1_1:27137315-27137334 None:intergenic 40.0%
AAAACCAATGGCAGAGTCAA+TGG - chr1_1:27137350-27137369 Msa0016330:three_prime_UTR 40.0%
AAGCATCTTGTGGCAATAGA+GGG + chr1_1:27137413-27137432 None:intergenic 40.0%
TAAGCATCTTGTGGCAATAG+AGG + chr1_1:27137414-27137433 None:intergenic 40.0%
!! AGTCTCTAGAATCCGATTGA+AGG - chr1_1:27137705-27137724 Msa0016330:CDS 40.0%
!! GTCTCTAGAATCCGATTGAA+GGG - chr1_1:27137706-27137725 Msa0016330:CDS 40.0%
! TTCATTGGTGGCAACACAAT+TGG - chr1_1:27138067-27138086 Msa0016330:intron 40.0%
AGTATGTGCAATCCAACCTA+AGG + chr1_1:27138107-27138126 None:intergenic 40.0%
CCAACAATTTCTTCCATGAC+TGG + chr1_1:27138133-27138152 None:intergenic 40.0%
CCAGTCATGGAAGAAATTGT+TGG - chr1_1:27138130-27138149 Msa0016330:intron 40.0%
AATTCTTGACCCAAGAGTGA+AGG + chr1_1:27138189-27138208 None:intergenic 40.0%
CTCTTGGGTCAAGAATTTCT+TGG - chr1_1:27138192-27138211 Msa0016330:intron 40.0%
TACTCAACAAGTAGCTCATG+GGG + chr1_1:27138247-27138266 None:intergenic 40.0%
GTACTCAACAAGTAGCTCAT+GGG + chr1_1:27138248-27138267 None:intergenic 40.0%
AGTACTCAACAAGTAGCTCA+TGG + chr1_1:27138249-27138268 None:intergenic 40.0%
! GCTACTTGTTGAGTACTTCA+AGG - chr1_1:27138252-27138271 Msa0016330:intron 40.0%
TTGAGTACTTCAAGGAAAGG+AGG - chr1_1:27138260-27138279 Msa0016330:intron 40.0%
TAAGTCGGTTGCTATGATCA+TGG + chr1_1:27138670-27138689 None:intergenic 40.0%
GTCAAGTCCATTTACTCAGA+GGG - chr1_1:27137200-27137219 Msa0016330:three_prime_UTR 40.0%
ACAGGAACCTTCTTGTAGAT+TGG + chr1_1:27137315-27137334 None:intergenic 40.0%
AAAACCAATGGCAGAGTCAA+TGG - chr1_1:27137350-27137369 Msa0016330:three_prime_UTR 40.0%
AAGCATCTTGTGGCAATAGA+GGG + chr1_1:27137413-27137432 None:intergenic 40.0%
TAAGCATCTTGTGGCAATAG+AGG + chr1_1:27137414-27137433 None:intergenic 40.0%
!! AGTCTCTAGAATCCGATTGA+AGG - chr1_1:27137705-27137724 Msa0016330:CDS 40.0%
!! GTCTCTAGAATCCGATTGAA+GGG - chr1_1:27137706-27137725 Msa0016330:CDS 40.0%
! TTCATTGGTGGCAACACAAT+TGG - chr1_1:27138067-27138086 Msa0016330:intron 40.0%
AGTATGTGCAATCCAACCTA+AGG + chr1_1:27138107-27138126 None:intergenic 40.0%
CCAACAATTTCTTCCATGAC+TGG + chr1_1:27138133-27138152 None:intergenic 40.0%
CCAGTCATGGAAGAAATTGT+TGG - chr1_1:27138130-27138149 Msa0016330:intron 40.0%
AATTCTTGACCCAAGAGTGA+AGG + chr1_1:27138189-27138208 None:intergenic 40.0%
CTCTTGGGTCAAGAATTTCT+TGG - chr1_1:27138192-27138211 Msa0016330:intron 40.0%
TACTCAACAAGTAGCTCATG+GGG + chr1_1:27138247-27138266 None:intergenic 40.0%
GTACTCAACAAGTAGCTCAT+GGG + chr1_1:27138248-27138267 None:intergenic 40.0%
AGTACTCAACAAGTAGCTCA+TGG + chr1_1:27138249-27138268 None:intergenic 40.0%
! GCTACTTGTTGAGTACTTCA+AGG - chr1_1:27138252-27138271 Msa0016330:intron 40.0%
TTGAGTACTTCAAGGAAAGG+AGG - chr1_1:27138260-27138279 Msa0016330:intron 40.0%
TAAGTCGGTTGCTATGATCA+TGG + chr1_1:27138670-27138689 None:intergenic 40.0%
GGTCAAGTCCATTTACTCAG+AGG - chr1_1:27137199-27137218 Msa0016330:three_prime_UTR 45.0%
CACACAACCCTCTGAGTAAA+TGG + chr1_1:27137210-27137229 None:intergenic 45.0%
CATTTACTCAGAGGGTTGTG+TGG - chr1_1:27137208-27137227 Msa0016330:three_prime_UTR 45.0%
!! ATCACCATTGACTCTGCCAT+TGG + chr1_1:27137357-27137376 None:intergenic 45.0%
TGCCACAAGATGCTTATGAG+AGG - chr1_1:27137418-27137437 Msa0016330:three_prime_UTR 45.0%
GACCTCTCATAAGCATCTTG+TGG + chr1_1:27137423-27137442 None:intergenic 45.0%
AGAGGTCCAATGTTCGTTTC+TGG - chr1_1:27137436-27137455 Msa0016330:three_prime_UTR 45.0%
GGGAAAACTAGCTACACAGA+AGG + chr1_1:27137500-27137519 None:intergenic 45.0%
CACATTACGTCGGCAGTATA+AGG + chr1_1:27137555-27137574 None:intergenic 45.0%
TGGTGGCAACACAATTGGAT+TGG - chr1_1:27138072-27138091 Msa0016330:intron 45.0%
ATTTCCCCACCTTCACTCTT+GGG - chr1_1:27138177-27138196 Msa0016330:intron 45.0%
GTACTTCAAGGAAAGGAGGA+AGG - chr1_1:27138264-27138283 Msa0016330:intron 45.0%
! TAACAATGACTGGAGAGTGC+AGG - chr1_1:27138504-27138523 Msa0016330:CDS 45.0%
GGTCAAGTCCATTTACTCAG+AGG - chr1_1:27137199-27137218 Msa0016330:three_prime_UTR 45.0%
CACACAACCCTCTGAGTAAA+TGG + chr1_1:27137210-27137229 None:intergenic 45.0%
CATTTACTCAGAGGGTTGTG+TGG - chr1_1:27137208-27137227 Msa0016330:three_prime_UTR 45.0%
!! ATCACCATTGACTCTGCCAT+TGG + chr1_1:27137357-27137376 None:intergenic 45.0%
TGCCACAAGATGCTTATGAG+AGG - chr1_1:27137418-27137437 Msa0016330:three_prime_UTR 45.0%
GACCTCTCATAAGCATCTTG+TGG + chr1_1:27137423-27137442 None:intergenic 45.0%
AGAGGTCCAATGTTCGTTTC+TGG - chr1_1:27137436-27137455 Msa0016330:three_prime_UTR 45.0%
GGGAAAACTAGCTACACAGA+AGG + chr1_1:27137500-27137519 None:intergenic 45.0%
CACATTACGTCGGCAGTATA+AGG + chr1_1:27137555-27137574 None:intergenic 45.0%
TGGTGGCAACACAATTGGAT+TGG - chr1_1:27138072-27138091 Msa0016330:intron 45.0%
ATTTCCCCACCTTCACTCTT+GGG - chr1_1:27138177-27138196 Msa0016330:intron 45.0%
GTACTTCAAGGAAAGGAGGA+AGG - chr1_1:27138264-27138283 Msa0016330:intron 45.0%
! TAACAATGACTGGAGAGTGC+AGG - chr1_1:27138504-27138523 Msa0016330:CDS 45.0%
!! ATAAAAGTAAAATAAAAAAT+AGG + chr1_1:27137776-27137795 None:intergenic 5.0%
!! ATAAAAGTAAAATAAAAAAT+AGG + chr1_1:27137776-27137795 None:intergenic 5.0%
TGTGGCAATAGAGGGATCTG+AGG + chr1_1:27137405-27137424 None:intergenic 50.0%
! GGATTGGCAGACATAGCCTT+AGG - chr1_1:27138088-27138107 Msa0016330:intron 50.0%
! TGGCAGACATAGCCTTAGGT+TGG - chr1_1:27138092-27138111 Msa0016330:intron 50.0%
TGCACATACTCTGCCAGTCA+TGG - chr1_1:27138117-27138136 Msa0016330:intron 50.0%
CATTTCCCCACCTTCACTCT+TGG - chr1_1:27138176-27138195 Msa0016330:intron 50.0%
TTGACCCAAGAGTGAAGGTG+GGG + chr1_1:27138184-27138203 None:intergenic 50.0%
CTTGACCCAAGAGTGAAGGT+GGG + chr1_1:27138185-27138204 None:intergenic 50.0%
TCTTGACCCAAGAGTGAAGG+TGG + chr1_1:27138186-27138205 None:intergenic 50.0%
TCAACAAGTAGCTCATGGGG+TGG + chr1_1:27138244-27138263 None:intergenic 50.0%
TGTGGCAATAGAGGGATCTG+AGG + chr1_1:27137405-27137424 None:intergenic 50.0%
! GGATTGGCAGACATAGCCTT+AGG - chr1_1:27138088-27138107 Msa0016330:intron 50.0%
! TGGCAGACATAGCCTTAGGT+TGG - chr1_1:27138092-27138111 Msa0016330:intron 50.0%
TGCACATACTCTGCCAGTCA+TGG - chr1_1:27138117-27138136 Msa0016330:intron 50.0%
CATTTCCCCACCTTCACTCT+TGG - chr1_1:27138176-27138195 Msa0016330:intron 50.0%
TTGACCCAAGAGTGAAGGTG+GGG + chr1_1:27138184-27138203 None:intergenic 50.0%
CTTGACCCAAGAGTGAAGGT+GGG + chr1_1:27138185-27138204 None:intergenic 50.0%
TCTTGACCCAAGAGTGAAGG+TGG + chr1_1:27138186-27138205 None:intergenic 50.0%
TCAACAAGTAGCTCATGGGG+TGG + chr1_1:27138244-27138263 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 27137171 27138732 27137171 ID=Msa0016330;Name=Msa0016330
chr1_1 mRNA 27137171 27138732 27137171 ID=Msa0016330-mRNA-1;Parent=Msa0016330;Name=Msa0016330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|411|226
chr1_1 exon 27137171 27137953 27137171 ID=Msa0016330-mRNA-1:exon:9154;Parent=Msa0016330-mRNA-1
chr1_1 exon 27138424 27138732 27138424 ID=Msa0016330-mRNA-1:exon:9153;Parent=Msa0016330-mRNA-1
chr1_1 CDS 27138424 27138732 27138424 ID=Msa0016330-mRNA-1:cds;Parent=Msa0016330-mRNA-1
chr1_1 CDS 27137582 27137953 27137582 ID=Msa0016330-mRNA-1:cds;Parent=Msa0016330-mRNA-1
chr1_1 three_prime_UTR 27137171 27137581 27137171 ID=Msa0016330-mRNA-1:three_prime_utr;Parent=Msa0016330-mRNA-1
Gene Sequence

>Msa0016330

ATGGAGGATGTTAAGGTTTTTGGTCTATGGTCAAGTCCATTTACTCAGAGGGTTGTGTGGACATTAAAAGTAAAGGGTGTGAGTTATGAGTATTATGGTGAAGATCTTGCAAATAAGAGTAACTTGTTACTGCAATACAATCCAATCTACAAGAAGGTTCCTGTTCTTGTACATAACGGAAAACCAATGGCAGAGTCAATGGTGATTGTTGAATATATTGATGAGACTTGGCCTCAGATCCCTCTATTGCCACAAGATGCTTATGAGAGGTCCAATGTTCGTTTCTGGTCAAAATTCATCGAGGAAAAGTCAATGCACATGATGGAATTTTTTCTGTACGATGGAGAACGACAAGAGAGATCAATAAAAGAAACTTTGGAAACTTTGAGAGTGATTGAAAAGGAAAGTGGGCTTAGTGAAAAGATGTTCATTGGTGGCAACACAATTGGATTGGCAGACATAGCCTTAGGTTGGATTGCACATACTCTGCCAGTCATGGAAGAAATTGTTGGAGTCAAGTTCATTACAGTTGATGCATTTCCCCACCTTCACTCTTGGGTCAAGAATTTCTTGGAAATTCCTGCTATAAAAAACAATCTTCCACCCCATGAGCTACTTGTTGAGTACTTCAAGGAAAGGAGGAAGGTGTTTCTTGCATTGACTTGTCATCATCACCATTAG
Protein sequence

>Msa0016330

MEDVKVFGLWSSPFTQRVVWTLKVKGVSYEYYGEDLANKSNLLLQYNPIYKKVPVLVHNGKPMAESMVIVEYIDETWPQIPLLPQDAYERSNVRFWSKFIEEKSMHMMEFFLYDGERQERSIKETLETLRVIEKESGLSEKMFIGGNTIGLADIALGWIAHTLPVMEEIVGVKFITVDAFPHLHSWVKNFLEIPAIKNNLPPHELLVEYFKERRKVFLALTCHHHH*