AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0016680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016680 MtrunA17_Chr3g0103751 91.892 74 6 0 8 81 1172 1245 1.82e-40 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016680 AT3G46960.3 72.973 74 20 0 8 81 1057 1130 2.65e-31 114
Msa0016680 AT3G46960.2 72.973 74 20 0 8 81 1048 1121 2.81e-31 114
Msa0016680 AT3G46960.1 72.973 74 20 0 8 81 1185 1258 3.52e-31 114

Find 30 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCAAAGGTGTACATTT+TGG 0.140890 1_1:-27740972 Msa0016680:three_prime_UTR
CTTCACTCTTCAGGGCTTAT+TGG 0.227219 1_1:+27741113 None:intergenic
GGGCATACTCTTCACTCTTC+AGG 0.230925 1_1:+27741104 None:intergenic
CAATCAAGTAAAGTTGTATT+TGG 0.256274 1_1:+27741010 None:intergenic
TATTTCTTAGTTAGTACATT+TGG 0.288529 1_1:-27743250 Msa0016680:five_prime_UTR
GCCCAAGAAAATATCAAATT+TGG 0.312676 1_1:-27741085 Msa0016680:three_prime_UTR
TTCACTCTTCAGGGCTTATT+GGG 0.325955 1_1:+27741114 None:intergenic
GGCATACTCTTCACTCTTCA+GGG 0.347658 1_1:+27741105 None:intergenic
ATTTCTTAGTTAGTACATTT+GGG 0.370811 1_1:-27743249 Msa0016680:five_prime_UTR
ATTAACACTGCTGCTTCTTC+TGG 0.386800 1_1:+27741438 None:intergenic
CTGGTTCCAGTTCATCGAGC+TGG 0.390858 1_1:+27741457 None:intergenic
CTAAATTCTATTCATATGTT+AGG 0.408564 1_1:+27740904 None:intergenic
TGAGAACCAGCTCGATGAAC+TGG 0.411981 1_1:-27741463 Msa0016680:CDS
CGTTGAAGTGGTTTATGAAT+GGG 0.412250 1_1:-27741059 Msa0016680:three_prime_UTR
TCGTTGAAGTGGTTTATGAA+TGG 0.458799 1_1:-27741060 Msa0016680:three_prime_UTR
GCTCGGATGCATTCTTCTGC+TGG 0.462768 1_1:+27741400 None:intergenic
AAGTGGTTTATGAATGGGAA+AGG 0.511580 1_1:-27741054 Msa0016680:three_prime_UTR
CAAATTTGGTCTCGTTGAAG+TGG 0.531565 1_1:-27741071 Msa0016680:three_prime_UTR
TGGACAACCACGACACTTGA+CGG 0.534623 1_1:+27743221 None:intergenic
GATTGCAAAAGCTATGAAGC+TGG 0.548585 1_1:-27742463 Msa0016680:exon
GGATTCGCCGTCAAGTGTCG+TGG 0.582573 1_1:-27743228 Msa0016680:five_prime_UTR
CTGTCTTACAGCACATCTCA+AGG 0.588076 1_1:-27743187 Msa0016680:intron
CCTAGGTGTGAGTGATGGCT+CGG 0.612561 1_1:+27741383 None:intergenic
GACAGCCTAGGTGTGAGTGA+TGG 0.618203 1_1:+27741378 None:intergenic
CCGAGCCATCACTCACACCT+AGG 0.627548 1_1:-27741383 Msa0016680:CDS
CACAAAATAGCTATACGACT+AGG 0.641406 1_1:-27741161 Msa0016680:CDS
GGGCATTACGTACCTCCTCA+CGG 0.645455 1_1:+27742436 None:intergenic
GTAAGACAGTTGAAGAAGTG+TGG 0.662636 1_1:+27743201 None:intergenic
GAAGCTGGAGTTCCGTGAGG+AGG 0.674460 1_1:-27742448 Msa0016680:intron
TATGAAGCTGGAGTTCCGTG+AGG 0.748586 1_1:-27742451 Msa0016680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATACTTGTATTTGAATTTT+CGG - chr1_1:27740995-27741014 Msa0016680:three_prime_UTR 15.0%
!!! ATGATTAGGTTTTTTTTTTT+TGG - chr1_1:27741441-27741460 Msa0016680:CDS 15.0%
!! AATAAAAAAAAAAACTAGCT+AGG + chr1_1:27741577-27741596 None:intergenic 15.0%
!!! ATATAATTTTTGTTTGGAAT+TGG - chr1_1:27741780-27741799 Msa0016680:intron 15.0%
!!! TATAATTTTTGTTTGGAATT+GGG - chr1_1:27741781-27741800 Msa0016680:intron 15.0%
!!! GATTAAACTATTTTAAGTAA+AGG - chr1_1:27742019-27742038 Msa0016680:intron 15.0%
!! AAGTTAAAAGAAAATGAAAA+TGG + chr1_1:27742332-27742351 None:intergenic 15.0%
!! GTTATAAAAAAAATTAAGAG+GGG + chr1_1:27742828-27742847 None:intergenic 15.0%
!! CGTTATAAAAAAAATTAAGA+GGG + chr1_1:27742829-27742848 None:intergenic 15.0%
!! ACGTTATAAAAAAAATTAAG+AGG + chr1_1:27742830-27742849 None:intergenic 15.0%
!! TATTTCTTAGTTAGTACATT+TGG - chr1_1:27740851-27740870 Msa0016680:three_prime_UTR 20.0%
!! ATTTCTTAGTTAGTACATTT+GGG - chr1_1:27740852-27740871 Msa0016680:three_prime_UTR 20.0%
!! ATGAACAATAAGATTAACTT+TGG + chr1_1:27741024-27741043 None:intergenic 20.0%
!!! GTTAATCAATTTTTTTCTCA+AGG - chr1_1:27741158-27741177 Msa0016680:CDS 20.0%
!! CAAAAAAATGTTAATCATAC+AGG + chr1_1:27741468-27741487 None:intergenic 20.0%
!!! ATATTTGTTTCTTCTGAAAA+AGG - chr1_1:27741607-27741626 Msa0016680:intron 20.0%
!!! CTGAACATATAATTTTTGTT+TGG - chr1_1:27741774-27741793 Msa0016680:intron 20.0%
!! AAGTAAACAATTCAATAAGA+AGG + chr1_1:27742133-27742152 None:intergenic 20.0%
!!! CTATATTCATTTTATGTGTT+TGG - chr1_1:27742580-27742599 Msa0016680:intron 20.0%
!!! ACCAAATTTGATATTTTCTT+GGG + chr1_1:27743020-27743039 None:intergenic 20.0%
!! CTAAATTCTATTCATATGTT+AGG + chr1_1:27743200-27743219 None:intergenic 20.0%
! ATTAAAACTAAGAGTAACAC+AGG + chr1_1:27740956-27740975 None:intergenic 25.0%
! TCTGTTAATCAATTGGTTAA+AGG - chr1_1:27741090-27741109 Msa0016680:three_prime_UTR 25.0%
! TTAATCAATTGGTTAAAGGA+AGG - chr1_1:27741094-27741113 Msa0016680:three_prime_UTR 25.0%
! TAATCAATTGGTTAAAGGAA+GGG - chr1_1:27741095-27741114 Msa0016680:three_prime_UTR 25.0%
! GAGAAAAAAATTGATTAACG+CGG + chr1_1:27741157-27741176 None:intergenic 25.0%
!! ATATTGCCAAACTATTTTGT+AGG - chr1_1:27741337-27741356 Msa0016680:intron 25.0%
!!! ATTGTGTTTTTGTTTTAGTC+TGG - chr1_1:27741886-27741905 Msa0016680:intron 25.0%
!!! TTGTAGATGTTGTTTTTACT+TGG - chr1_1:27741932-27741951 Msa0016680:intron 25.0%
! CAATCCTAACATATTTGTAT+AGG - chr1_1:27742374-27742393 Msa0016680:intron 25.0%
!! GACCAAATTTGATATTTTCT+TGG + chr1_1:27743021-27743040 None:intergenic 25.0%
! CAATCAAGTAAAGTTGTATT+TGG + chr1_1:27743094-27743113 None:intergenic 25.0%
!!! AGCATAGTTTTTAAGATCAA+AGG - chr1_1:27743117-27743136 Msa0016680:intron 25.0%
ATATATCCATGTGTATGTGA+TGG - chr1_1:27741054-27741073 Msa0016680:three_prime_UTR 30.0%
ACTTGTCTCTGTTAATCAAT+TGG - chr1_1:27741083-27741102 Msa0016680:three_prime_UTR 30.0%
AATCAATTGGTTAAAGGAAG+GGG - chr1_1:27741096-27741115 Msa0016680:exon 30.0%
!! GAATTTGTTAACAGTCTAGA+TGG - chr1_1:27741119-27741138 Msa0016680:CDS 30.0%
TTTATAGAACTTTGTGTGTG+TGG - chr1_1:27741230-27741249 Msa0016680:intron 30.0%
AGCATTCCTACAAAATAGTT+TGG + chr1_1:27741346-27741365 None:intergenic 30.0%
GAATTAGAGAGAAGAATCAA+AGG + chr1_1:27741373-27741392 None:intergenic 30.0%
TATATGTTCAGAATCACAGT+TGG + chr1_1:27741765-27741784 None:intergenic 30.0%
ACTGATGTGTAGAAAGAAAA+AGG - chr1_1:27741837-27741856 Msa0016680:intron 30.0%
CTGATGTGTAGAAAGAAAAA+GGG - chr1_1:27741838-27741857 Msa0016680:intron 30.0%
! TGGTTGTTGTTCTTATTGAA+AGG - chr1_1:27741906-27741925 Msa0016680:intron 30.0%
! GATATGAAGTGTTCTAATTC+CGG - chr1_1:27741972-27741991 Msa0016680:intron 30.0%
CAAGATGAAAATGACAGTAA+TGG - chr1_1:27742056-27742075 Msa0016680:intron 30.0%
TTCATCGTGTAAAATTTGTG+TGG - chr1_1:27742165-27742184 Msa0016680:intron 30.0%
AAACAAGAATTCAAGTATCC+TGG + chr1_1:27742210-27742229 None:intergenic 30.0%
ATCATCGTTCAATTGGTTAA+AGG - chr1_1:27742269-27742288 Msa0016680:intron 30.0%
TTCAATTGGTTAAAGGAAGA+GGG - chr1_1:27742276-27742295 Msa0016680:intron 30.0%
!! TAGGATTGTGTAAAAGTGTT+GGG + chr1_1:27742362-27742381 None:intergenic 30.0%
! TTAGGATTGTGTAAAAGTGT+TGG + chr1_1:27742363-27742382 None:intergenic 30.0%
AGTGCCTATACAAATATGTT+AGG + chr1_1:27742381-27742400 None:intergenic 30.0%
AACATATTTGTATAGGCACT+AGG - chr1_1:27742381-27742400 Msa0016680:intron 30.0%
TGTGTTCGGTTAAAAGTAAT+AGG - chr1_1:27742407-27742426 Msa0016680:intron 30.0%
TTCTGATTGGAATCAACATA+TGG - chr1_1:27742888-27742907 Msa0016680:intron 30.0%
GCCCAAGAAAATATCAAATT+TGG - chr1_1:27743016-27743035 Msa0016680:intron 30.0%
!! AAGATCAAAGGTGTACATTT+TGG - chr1_1:27743129-27743148 Msa0016680:intron 30.0%
!! AGATCAAAGGTGTACATTTT+GGG - chr1_1:27743130-27743149 Msa0016680:intron 30.0%
AGTAACACAGGTAAAGAGAA+GGG + chr1_1:27740944-27740963 None:intergenic 35.0%
ATCAATTGGTTAAAGGAAGG+GGG - chr1_1:27741097-27741116 Msa0016680:exon 35.0%
ACAAGAATAAGATCGCGTTT+AGG + chr1_1:27741492-27741511 None:intergenic 35.0%
! TTCATGGGAGGAACAATTTT+TGG + chr1_1:27741693-27741712 None:intergenic 35.0%
!!! CAGTTGCTTTTGCAAAATCA+CGG - chr1_1:27741720-27741739 Msa0016680:intron 35.0%
CTTTGACATCATCGTTCAAT+TGG - chr1_1:27742262-27742281 Msa0016680:intron 35.0%
GTTCAATTGGTTAAAGGAAG+AGG - chr1_1:27742275-27742294 Msa0016680:intron 35.0%
!!! CATAGTCTAATGCCACTTTT+CGG - chr1_1:27742480-27742499 Msa0016680:intron 35.0%
AATCAGAATTTCAGAGAAGC+AGG + chr1_1:27742876-27742895 None:intergenic 35.0%
!! TGCTTCTCTGAAATTCTGAT+TGG - chr1_1:27742875-27742894 Msa0016680:intron 35.0%
CACAAAATAGCTATACGACT+AGG - chr1_1:27742940-27742959 Msa0016680:intron 35.0%
TCGTTGAAGTGGTTTATGAA+TGG - chr1_1:27743041-27743060 Msa0016680:intron 35.0%
CGTTGAAGTGGTTTATGAAT+GGG - chr1_1:27743042-27743061 Msa0016680:intron 35.0%
AAGTGGTTTATGAATGGGAA+AGG - chr1_1:27743047-27743066 Msa0016680:intron 35.0%
!! GATCAAAGGTGTACATTTTG+GGG - chr1_1:27743131-27743150 Msa0016680:intron 35.0%
GTAAGACAGTTGAAGAAGTG+TGG + chr1_1:27740903-27740922 None:intergenic 40.0%
GAGTAACACAGGTAAAGAGA+AGG + chr1_1:27740945-27740964 None:intergenic 40.0%
TGAAGACCATCACATACACA+TGG + chr1_1:27741063-27741082 None:intergenic 40.0%
CTATCTAATGCAAGTGAGTG+TGG - chr1_1:27741256-27741275 Msa0016680:intron 40.0%
ACGCGTCAAGTTCTATGATT+AGG - chr1_1:27741427-27741446 Msa0016680:CDS 40.0%
GATTGCAAAAGCTATGAAGC+TGG - chr1_1:27741638-27741657 Msa0016680:intron 40.0%
!!! TGGGAGGAACAATTTTTGGT+CGG + chr1_1:27741689-27741708 None:intergenic 40.0%
GAAAAAGGGATGAGCTTGAT+TGG - chr1_1:27741852-27741871 Msa0016680:intron 40.0%
CCTACAAGAACAACATCAAC+CGG + chr1_1:27741994-27742013 None:intergenic 40.0%
AAGAATTCAAGTATCCTGGC+CGG + chr1_1:27742206-27742225 None:intergenic 40.0%
GCAAAATACCAACCGAAAAG+TGG + chr1_1:27742495-27742514 None:intergenic 40.0%
! ATTAACACTGCTGCTTCTTC+TGG + chr1_1:27742666-27742685 None:intergenic 40.0%
!!! TTTTTTGCATCAGACAGCCT+AGG + chr1_1:27742738-27742757 None:intergenic 40.0%
GGCTGTCTGATGCAAAAAAT+AGG - chr1_1:27742739-27742758 Msa0016680:intron 40.0%
TTCACTCTTCAGGGCTTATT+GGG + chr1_1:27742990-27743009 None:intergenic 40.0%
CAAATTTGGTCTCGTTGAAG+TGG - chr1_1:27743030-27743049 Msa0016680:intron 40.0%
! CACCAATACATTTTTCACCG+AGG + chr1_1:27743157-27743176 None:intergenic 40.0%
CTGTCTTACAGCACATCTCA+AGG - chr1_1:27740914-27740933 Msa0016680:three_prime_UTR 45.0%
!!! GGGAGGAACAATTTTTGGTC+GGG + chr1_1:27741688-27741707 None:intergenic 45.0%
AGCAACTGAGCTCGATTCAT+GGG + chr1_1:27741708-27741727 None:intergenic 45.0%
AAGCAACTGAGCTCGATTCA+TGG + chr1_1:27741709-27741728 None:intergenic 45.0%
CAGTTGGTTAGCATCACTGT+AGG + chr1_1:27741749-27741768 None:intergenic 45.0%
! CCGGTTGATGTTGTTCTTGT+AGG - chr1_1:27741991-27742010 Msa0016680:intron 45.0%
CCAGCACTTAGCTTCTTAAC+AGG + chr1_1:27742086-27742105 None:intergenic 45.0%
CCTGTTAAGAAGCTAAGTGC+TGG - chr1_1:27742083-27742102 Msa0016680:intron 45.0%
AGAATTCAAGTATCCTGGCC+GGG + chr1_1:27742205-27742224 None:intergenic 45.0%
TAGGCACTAGGCAATGTGTT+CGG - chr1_1:27742393-27742412 Msa0016680:intron 45.0%
!!! GTCTAATGCCACTTTTCGGT+TGG - chr1_1:27742484-27742503 Msa0016680:intron 45.0%
CTTCACTCTTCAGGGCTTAT+TGG + chr1_1:27742991-27743010 None:intergenic 45.0%
GGCATACTCTTCACTCTTCA+GGG + chr1_1:27742999-27743018 None:intergenic 45.0%
GGCCTCGGTGAAAAATGTAT+TGG - chr1_1:27743152-27743171 Msa0016680:intron 45.0%
TGGACAACCACGACACTTGA+CGG + chr1_1:27740883-27740902 None:intergenic 50.0%
TATGAAGCTGGAGTTCCGTG+AGG - chr1_1:27741650-27741669 Msa0016680:intron 50.0%
AACTGAGCTCGATTCATGGG+AGG + chr1_1:27741705-27741724 None:intergenic 50.0%
AATTCAAGTATCCTGGCCGG+GGG + chr1_1:27742203-27742222 None:intergenic 50.0%
GAATTCAAGTATCCTGGCCG+GGG + chr1_1:27742204-27742223 None:intergenic 50.0%
TGAGAACCAGCTCGATGAAC+TGG - chr1_1:27742638-27742657 Msa0016680:intron 50.0%
GGGCATACTCTTCACTCTTC+AGG + chr1_1:27743000-27743019 None:intergenic 50.0%
!!! AGGTGTACATTTTGGGGCCT+CGG - chr1_1:27743137-27743156 Msa0016680:intron 50.0%
GTGAGTGTGGTGTGTGTGTG+TGG - chr1_1:27741269-27741288 Msa0016680:intron 55.0%
GGGCATTACGTACCTCCTCA+CGG + chr1_1:27741668-27741687 None:intergenic 55.0%
GGCGAGTTGCGTCTTCACAA+AGG - chr1_1:27742104-27742123 Msa0016680:intron 55.0%
CTGGTTCCAGTTCATCGAGC+TGG + chr1_1:27742647-27742666 None:intergenic 55.0%
GCTCGGATGCATTCTTCTGC+TGG + chr1_1:27742704-27742723 None:intergenic 55.0%
CCTAGGTGTGAGTGATGGCT+CGG + chr1_1:27742721-27742740 None:intergenic 55.0%
GACAGCCTAGGTGTGAGTGA+TGG + chr1_1:27742726-27742745 None:intergenic 55.0%
! GGATTCGCCGTCAAGTGTCG+TGG - chr1_1:27740873-27740892 Msa0016680:three_prime_UTR 60.0%
GAAGCTGGAGTTCCGTGAGG+AGG - chr1_1:27741653-27741672 Msa0016680:intron 60.0%
AAGTATCCTGGCCGGGGGTA+GGG + chr1_1:27742198-27742217 None:intergenic 60.0%
CCGAGCCATCACTCACACCT+AGG - chr1_1:27742718-27742737 Msa0016680:intron 60.0%
AGTATCCTGGCCGGGGGTAG+GGG + chr1_1:27742197-27742216 None:intergenic 65.0%
CAAGTATCCTGGCCGGGGGT+AGG + chr1_1:27742199-27742218 None:intergenic 65.0%
GTGGCACTTGCCCCTACCCC+CGG - chr1_1:27742184-27742203 Msa0016680:intron 70.0%
ACTTGCCCCTACCCCCGGCC+AGG - chr1_1:27742189-27742208 Msa0016680:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 27740834 27743289 27740834 ID=Msa0016680;Name=Msa0016680
chr1_1 mRNA 27740834 27743289 27740834 ID=Msa0016680-mRNA-1;Parent=Msa0016680;Name=Msa0016680-mRNA-1;_AED=0.32;_eAED=0.32;_QI=124|0.66|0.75|1|0.66|0.5|4|281|81
chr1_1 exon 27740834 27741187 27740834 ID=Msa0016680-mRNA-1:exon:9308;Parent=Msa0016680-mRNA-1
chr1_1 exon 27741363 27741511 27741363 ID=Msa0016680-mRNA-1:exon:9307;Parent=Msa0016680-mRNA-1
chr1_1 exon 27742449 27742494 27742449 ID=Msa0016680-mRNA-1:exon:9306;Parent=Msa0016680-mRNA-1
chr1_1 exon 27743188 27743289 27743188 ID=Msa0016680-mRNA-1:exon:9305;Parent=Msa0016680-mRNA-1
chr1_1 five_prime_UTR 27743188 27743289 27743188 ID=Msa0016680-mRNA-1:five_prime_utr;Parent=Msa0016680-mRNA-1
chr1_1 five_prime_UTR 27742473 27742494 27742473 ID=Msa0016680-mRNA-1:five_prime_utr;Parent=Msa0016680-mRNA-1
chr1_1 CDS 27742449 27742472 27742449 ID=Msa0016680-mRNA-1:cds;Parent=Msa0016680-mRNA-1
chr1_1 CDS 27741363 27741511 27741363 ID=Msa0016680-mRNA-1:cds;Parent=Msa0016680-mRNA-1
chr1_1 CDS 27741115 27741187 27741115 ID=Msa0016680-mRNA-1:cds;Parent=Msa0016680-mRNA-1
chr1_1 three_prime_UTR 27740834 27741114 27740834 ID=Msa0016680-mRNA-1:three_prime_utr;Parent=Msa0016680-mRNA-1
Gene Sequence

>Msa0016680

ATGAAGCTGGAGTTCCGTGAGGAGTCTCTGATATGTACTGAGTGCTTGTTTGAGAACCAGCTCGATGAACTGGAACCAGAAGAAGCAGCAGTGTTAATGTCTGCTTTTGTGTTCCAGCAGAAGAATGCATCCGAGCCATCACTCACACCTAGGCTGTCTGATGCAAAAAATAGATTGCACAAAATAGCTATACGACTAGGAGAGCTTCAAGCACAGTTTAATCTCCCAATAAGCCCTGAAGAGTGA

Protein sequence

>Msa0016680

MKLEFREESLICTECLFENQLDELEPEEAAVLMSAFVFQQKNASEPSLTPRLSDAKNRLHKIAIRLGELQAQFNLPISPEE*