AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0016740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016740 MtrunA17_Chr2g0299671 91.837 98 7 1 4 100 218 315 3.89e-54 180
Msa0016740 MtrunA17_Chr4g0070991 73.469 98 25 1 3 100 189 285 8.14e-43 149
Msa0016740 MtrunA17_Chr8g0385411 80.822 73 8 1 125 191 159 231 4.96e-33 124
Msa0016740 MtrunA17_Chr3g0131401 56.667 60 26 0 132 191 193 252 9.59e-18 80.5
Msa0016740 MtrunA17_Chr1g0192121 58.333 60 24 1 132 191 161 219 5.85e-15 72.4
Msa0016740 MtrunA17_Chr7g0272591 56.667 60 25 1 132 191 174 232 6.39e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016740 AT2G19450.1 67.010 97 25 1 4 100 208 297 3.95e-34 126
Msa0016740 AT5G47910.1 47.692 65 34 0 127 191 200 264 1.03e-13 69.3
Msa0016740 AT5G51060.1 60.000 50 20 0 142 191 188 237 1.71e-12 65.5

Find 84 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGTTGGTGATATGTTTA+TGG 0.214566 1_1:+27869454 None:intergenic
CCTCTTCATGTGTTGGTAAT+GGG 0.237600 1_1:-27871613 Msa0016740:five_prime_UTR
TGTCTATTCTGTCCTCCTTT+TGG 0.286716 1_1:-27870057 Msa0016740:CDS
GGTTGATTCGATCTGGCTTT+TGG 0.288503 1_1:-27871665 Msa0016740:five_prime_UTR
CAGACCAAAGCTTTGATACT+AGG 0.290247 1_1:-27869546 Msa0016740:CDS
AGATCCAGTTTAGGGAGTTC+TGG 0.296332 1_1:-27869579 Msa0016740:CDS
CAAGAGTTTAGCATACTTCA+TGG 0.303155 1_1:-27870425 Msa0016740:CDS
CCCTCTTCATGTGTTGGTAA+TGG 0.317436 1_1:-27871614 Msa0016740:five_prime_UTR
CGATTAACAAGATCCAGTTT+AGG 0.323576 1_1:-27869588 Msa0016740:CDS
TTGCATAGTGTGGTTAAAAT+TGG 0.337241 1_1:-27870827 Msa0016740:CDS
GATTAACAAGATCCAGTTTA+GGG 0.337450 1_1:-27869587 Msa0016740:CDS
AGTGCCTGATACTTTGAATA+TGG 0.344744 1_1:-27870470 Msa0016740:CDS
AAAGTTGATTTGAATGTTGC+AGG 0.358644 1_1:-27871561 Msa0016740:exon
TACTCCATATTCAAAGTATC+AGG 0.365199 1_1:+27870466 None:intergenic
ATTCGGCCATGTATATGATA+TGG 0.369935 1_1:-27869381 Msa0016740:three_prime_UTR
TATTATGTGATGTACTAATT+CGG 0.370244 1_1:-27869398 Msa0016740:three_prime_UTR
CAGAGTGAGAGGGAGAAGAA+AGG 0.375249 1_1:+27871992 None:intergenic
GACCTGAGGATGACTAAAAC+TGG 0.382726 1_1:+27871491 None:intergenic
GTTCAAAATCGCTGAGAGAT+TGG 0.390939 1_1:-27871638 Msa0016740:five_prime_UTR
GACAAAATGCATCAAAGATC+AGG 0.394937 1_1:+27869995 None:intergenic
TGAGCCTAGTATCAAAGCTT+TGG 0.396068 1_1:+27869542 None:intergenic
AGTCCCAGAACTCCCTAAAC+TGG 0.398261 1_1:+27869575 None:intergenic
AGAGTGAGAGGGAGAAGAAA+GGG 0.404714 1_1:+27871993 None:intergenic
CTCTGCCTCTCACTCACTTC+CGG 0.419111 1_1:-27871974 Msa0016740:five_prime_UTR
AAAATACCCATGCCACATAA+GGG 0.419152 1_1:+27872067 None:intergenic
GATCCAGTTTAGGGAGTTCT+GGG 0.425992 1_1:-27869578 Msa0016740:CDS
TATTATTGAAAATCTGATGA+AGG 0.454922 1_1:-27871814 Msa0016740:intron
CAAACTACATCTACCGCAGC+TGG 0.465385 1_1:+27869972 None:intergenic
TATGGATGGTTGATTCGATC+TGG 0.468829 1_1:-27871672 Msa0016740:five_prime_UTR
GTTACCTGGTAGCATAATGT+AGG 0.471407 1_1:+27870397 None:intergenic
CATGCCACATAAGGGGTGTA+TGG 0.473033 1_1:+27872075 None:intergenic
CTCTTGAAGTTCACATTGTG+TGG 0.476660 1_1:+27870442 None:intergenic
TGAGTGAGAGGCAGAGTGAG+AGG 0.480903 1_1:+27871981 None:intergenic
TTTGACACTTACATTAATGT+TGG 0.483582 1_1:+27869440 None:intergenic
GCTCGGCAGAGAGGGATTCA+AGG 0.486020 1_1:-27869616 Msa0016740:CDS
GAGATCTAAGAGAGAGAAGA+GGG 0.490981 1_1:+27872015 None:intergenic
AGCAAGCGCTCTCATATCAT+AGG 0.491205 1_1:+27870786 None:intergenic
AGGGAGAGAGTGGGAACGAC+CGG 0.501155 1_1:+27871955 None:intergenic
TGCTTGTTGCGGTGAATAGT+AGG 0.505850 1_1:-27871839 Msa0016740:five_prime_UTR
AAGCTAAGCAGATGACCAAA+AGG 0.508120 1_1:+27870042 None:intergenic
ATTTACTCATATCATTTACA+TGG 0.515511 1_1:+27869319 None:intergenic
GAGAGTGGGAGGAGAAGGCC+AGG 0.527059 1_1:+27871914 None:intergenic
GAGTGAGAGGCAGAGTGAGA+GGG 0.530635 1_1:+27871982 None:intergenic
ACTGTGAGAGTGGGAGGAGA+AGG 0.535318 1_1:+27871909 None:intergenic
ATACTCTTGCTCGGCAGAGA+GGG 0.537758 1_1:-27869624 Msa0016740:CDS
GGAGATCTAAGAGAGAGAAG+AGG 0.543617 1_1:+27872014 None:intergenic
CTGTATTGTGGTGCTTGTTG+CGG 0.545649 1_1:-27871850 Msa0016740:five_prime_UTR
CATACACCCCTTATGTGGCA+TGG 0.546542 1_1:-27872074 Msa0016740:five_prime_UTR
TGGAAGAGTGGTATGGAGAA+GGG 0.549448 1_1:+27872095 None:intergenic
CCCATTACCAACACATGAAG+AGG 0.550568 1_1:+27871613 None:intergenic
TCTTCCATACACCCCTTATG+TGG 0.557932 1_1:-27872079 Msa0016740:five_prime_UTR
GTCGGCCGTCAGAGTCATGC+AGG 0.562411 1_1:-27871885 Msa0016740:five_prime_UTR
TCACTGTTTAAGGGAGAGAG+TGG 0.562567 1_1:+27871945 None:intergenic
ACGGCCGACGACTGTGAGAG+TGG 0.564111 1_1:+27871899 None:intergenic
ATTGGCCCCTCTTCATGTGT+TGG 0.564337 1_1:-27871620 Msa0016740:five_prime_UTR
GGGTGTATGGAAGAGTGGTA+TGG 0.569311 1_1:+27872088 None:intergenic
ATACACCCCTTATGTGGCAT+GGG 0.571567 1_1:-27872073 Msa0016740:five_prime_UTR
TGCTCCTACATTATGCTACC+AGG 0.572609 1_1:-27870401 Msa0016740:intron
ATGGAAGAGTGGTATGGAGA+AGG 0.576479 1_1:+27872094 None:intergenic
AGGATTGAATGAAGAGTCAA+AGG 0.577878 1_1:-27869668 Msa0016740:CDS
CACTGTTTAAGGGAGAGAGT+GGG 0.578733 1_1:+27871946 None:intergenic
AGTTAGCCATATCATATACA+TGG 0.591734 1_1:+27869375 None:intergenic
TGCTATTGACTTGCATAGTG+TGG 0.598533 1_1:-27870837 Msa0016740:CDS
ACAAAATGCATCAAAGATCA+GGG 0.602070 1_1:+27869996 None:intergenic
TACTCATATCATTTACATGG+TGG 0.610543 1_1:+27869322 None:intergenic
ATAAGGGGTGTATGGAAGAG+TGG 0.614419 1_1:+27872083 None:intergenic
TCTTGAAGTTCACATTGTGT+GGG 0.616470 1_1:+27870443 None:intergenic
AAACTACATCTACCGCAGCT+GGG 0.618191 1_1:+27869973 None:intergenic
CCATTACCAACACATGAAGA+GGG 0.619542 1_1:+27871614 None:intergenic
AACGACCGGAAGTGAGTGAG+AGG 0.624026 1_1:+27871969 None:intergenic
AAATACCCATGCCACATAAG+GGG 0.631006 1_1:+27872068 None:intergenic
CGGCCGACGACTGTGAGAGT+GGG 0.631495 1_1:+27871900 None:intergenic
CCGACGACTGTGAGAGTGGG+AGG 0.636823 1_1:+27871903 None:intergenic
TAAACAGTGAAAACTCAACC+TGG 0.641654 1_1:-27871932 Msa0016740:five_prime_UTR
ACTGTTCAATCTCTGTATTG+TGG 0.642176 1_1:-27871862 Msa0016740:five_prime_UTR
GCTTGCTGTTTCAAATGACA+AGG 0.642596 1_1:-27870770 Msa0016740:intron
CCTCCCACTCTCACAGTCGT+CGG 0.654037 1_1:-27871903 Msa0016740:five_prime_UTR
GATACTCTTGCTCGGCAGAG+AGG 0.662539 1_1:-27869625 Msa0016740:CDS
ACAGTCCTGCATGACTCTGA+CGG 0.673299 1_1:+27871880 None:intergenic
CTAAGCAGATGACCAAAAGG+AGG 0.683064 1_1:+27870045 None:intergenic
CGGCAGAGAGGGATTCAAGG+AGG 0.710701 1_1:-27869613 Msa0016740:CDS
CATTACCAACACATGAAGAG+GGG 0.715726 1_1:+27871615 None:intergenic
TCAGGGTCAAAACCCAACAG+AGG 0.721005 1_1:+27870013 None:intergenic
GCAAGTCAATAGCATCAACG+TGG 0.767827 1_1:+27870846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTTAAACATTGATT+TGG - chr1_1:27870417-27870436 Msa0016740:CDS 10.0%
!! TGAATAATATCTATAATAAA+AGG - chr1_1:27870453-27870472 Msa0016740:CDS 10.0%
!!! CTTATTTATGACTATATTTT+TGG - chr1_1:27871195-27871214 Msa0016740:intron 15.0%
!! TATTATTGAAAATCTGATGA+AGG - chr1_1:27869604-27869623 Msa0016740:CDS 20.0%
!!! AAAGCATTTTTTTTACAAGT+TGG + chr1_1:27869660-27869679 None:intergenic 20.0%
!!! TAATTATATGTTTTGCAGTA+TGG - chr1_1:27869728-27869747 Msa0016740:intron 20.0%
!!! TTTTAAGTCTATTCTTAAAG+AGG + chr1_1:27869840-27869859 None:intergenic 20.0%
!! ATGAAATTATGTCAATATCT+AGG - chr1_1:27870080-27870099 Msa0016740:intron 20.0%
!! AATATAATTAGAAACACAGA+AGG - chr1_1:27870218-27870237 Msa0016740:intron 20.0%
!! AGTATTTCGATAAATATAAG+AGG - chr1_1:27870679-27870698 Msa0016740:intron 20.0%
!!! TTATGACTATATTTTTGGTT+TGG - chr1_1:27871200-27871219 Msa0016740:intron 20.0%
!!! AAATTTTTCCTTCAATTTGT+GGG - chr1_1:27871598-27871617 Msa0016740:five_prime_UTR 20.0%
!! TATTATGTGATGTACTAATT+CGG - chr1_1:27872020-27872039 Msa0016740:five_prime_UTR 20.0%
!! ATTTACTCATATCATTTACA+TGG + chr1_1:27872102-27872121 None:intergenic 20.0%
!! AGTTGGAAATTCAAATTTTG+CGG + chr1_1:27869643-27869662 None:intergenic 25.0%
! AATTATGTCAATATCTAGGA+AGG - chr1_1:27870084-27870103 Msa0016740:intron 25.0%
! ATTATGTCAATATCTAGGAA+GGG - chr1_1:27870085-27870104 Msa0016740:intron 25.0%
! ATATTCATGTCATGTGATAA+AGG - chr1_1:27870291-27870310 Msa0016740:intron 25.0%
! CTATAATAAAAGGTTCTTCT+TGG - chr1_1:27870463-27870482 Msa0016740:CDS 25.0%
!! AGTTAAAAACTTTTGTTACC+TGG + chr1_1:27871038-27871057 None:intergenic 25.0%
! AAATAAATGCATGCAAATGT+CGG + chr1_1:27871137-27871156 None:intergenic 25.0%
!! AAAATTTGAGATAAGAGCTA+GGG + chr1_1:27871585-27871604 None:intergenic 25.0%
!! AAAAATTTGAGATAAGAGCT+AGG + chr1_1:27871586-27871605 None:intergenic 25.0%
!! CAAATTTTTCCTTCAATTTG+TGG - chr1_1:27871597-27871616 Msa0016740:five_prime_UTR 25.0%
! GTAATTTCTAAGTGTTTCTT+TGG - chr1_1:27871717-27871736 Msa0016740:intron 25.0%
!!! TAATGTTGGTGATATGTTTA+TGG + chr1_1:27871967-27871986 None:intergenic 25.0%
! TTTGACACTTACATTAATGT+TGG + chr1_1:27871981-27872000 None:intergenic 25.0%
! GTTGAGTTTTCACTGTTTAA+GGG + chr1_1:27869485-27869504 None:intergenic 30.0%
! TATATGTTTTGCAGTATGGA+TGG - chr1_1:27869732-27869751 Msa0016740:intron 30.0%
!! AAAGTTGATTTGAATGTTGC+AGG - chr1_1:27869857-27869876 Msa0016740:intron 30.0%
! ATTAGTTTAGATAGAAGTCG+AGG + chr1_1:27869975-27869994 None:intergenic 30.0%
AAAAGTTTCAAAGCTGTTTG+AGG - chr1_1:27870139-27870158 Msa0016740:intron 30.0%
AAACACAGAAGGTAATTTCA+AGG - chr1_1:27870229-27870248 Msa0016740:intron 30.0%
AACACAGAAGGTAATTTCAA+GGG - chr1_1:27870230-27870249 Msa0016740:intron 30.0%
ATTTCAAGGGAACAAACAAT+TGG - chr1_1:27870243-27870262 Msa0016740:intron 30.0%
TTTCAAGGGAACAAACAATT+GGG - chr1_1:27870244-27870263 Msa0016740:intron 30.0%
! TTTTATGCTCAAGAAACACA+GGG + chr1_1:27870379-27870398 None:intergenic 30.0%
!! AACATTGATTTGGCTTATGA+TGG - chr1_1:27870427-27870446 Msa0016740:CDS 30.0%
GGTATTGTGAAGATATCTTA+TGG - chr1_1:27870502-27870521 Msa0016740:intron 30.0%
TTGCATAGTGTGGTTAAAAT+TGG - chr1_1:27870591-27870610 Msa0016740:intron 30.0%
GTTATGCTATATGTTCTATG+TGG - chr1_1:27870701-27870720 Msa0016740:intron 30.0%
AGGTAACATGATTGAATAAG+GGG + chr1_1:27870911-27870930 None:intergenic 30.0%
CAGGTAACATGATTGAATAA+GGG + chr1_1:27870912-27870931 None:intergenic 30.0%
ACAGGTAACATGATTGAATA+AGG + chr1_1:27870913-27870932 None:intergenic 30.0%
TTATTCAATCATGTTACCTG+TGG - chr1_1:27870912-27870931 Msa0016740:intron 30.0%
! TACTCCATATTCAAAGTATC+AGG + chr1_1:27870955-27870974 None:intergenic 30.0%
!!! CATTAGTGGATTTTTTCCTA+TGG - chr1_1:27871292-27871311 Msa0016740:intron 30.0%
ACAAAATGCATCAAAGATCA+GGG + chr1_1:27871425-27871444 None:intergenic 30.0%
GCTTATTTGCATGTACAATT+TGG - chr1_1:27871530-27871549 Msa0016740:CDS 30.0%
!!! GGGATTTTGTGTTTTGAAAA+TGG - chr1_1:27871639-27871658 Msa0016740:five_prime_UTR 30.0%
GTTTCTTTGGATTCAATTGT+AGG - chr1_1:27871730-27871749 Msa0016740:intron 30.0%
GATTAACAAGATCCAGTTTA+GGG - chr1_1:27871831-27871850 Msa0016740:five_prime_UTR 30.0%
AGTTAGCCATATCATATACA+TGG + chr1_1:27872046-27872065 None:intergenic 30.0%
TACTCATATCATTTACATGG+TGG + chr1_1:27872099-27872118 None:intergenic 30.0%
AAAATACCCATGCCACATAA+GGG + chr1_1:27869354-27869373 None:intergenic 35.0%
AAAAATACCCATGCCACATA+AGG + chr1_1:27869355-27869374 None:intergenic 35.0%
! GGTTGAGTTTTCACTGTTTA+AGG + chr1_1:27869486-27869505 None:intergenic 35.0%
TAAACAGTGAAAACTCAACC+TGG - chr1_1:27869486-27869505 Msa0016740:three_prime_UTR 35.0%
ACTGTTCAATCTCTGTATTG+TGG - chr1_1:27869556-27869575 Msa0016740:CDS 35.0%
TTTAAGTCTATACTGACCTG+AGG + chr1_1:27869944-27869963 None:intergenic 35.0%
!! AGGAACCTCATTTTAGTGTT+TGG - chr1_1:27870159-27870178 Msa0016740:intron 35.0%
ACGAACCAAACACTAAAATG+AGG + chr1_1:27870167-27870186 None:intergenic 35.0%
AACAATTGGGAACAACTTTG+TGG - chr1_1:27870257-27870276 Msa0016740:intron 35.0%
GTTACATGAATGTCTGTCAA+AGG + chr1_1:27870317-27870336 None:intergenic 35.0%
! CTTTTATGCTCAAGAAACAC+AGG + chr1_1:27870380-27870399 None:intergenic 35.0%
ATAAAAGTTGAGCACATCTG+TGG - chr1_1:27870393-27870412 Msa0016740:intron 35.0%
GTTCTTCTTGGTCATTTCAT+GGG - chr1_1:27870475-27870494 Msa0016740:CDS 35.0%
!!! TGCGATTCTGCTTTTTTATC+TGG - chr1_1:27870547-27870566 Msa0016740:intron 35.0%
AATCATGTTACCTGTGGTAT+AGG - chr1_1:27870918-27870937 Msa0016740:intron 35.0%
ATCATGTTACCTGTGGTATA+GGG - chr1_1:27870919-27870938 Msa0016740:intron 35.0%
AGTGCCTGATACTTTGAATA+TGG - chr1_1:27870948-27870967 Msa0016740:intron 35.0%
TCTTGAAGTTCACATTGTGT+GGG + chr1_1:27870978-27870997 None:intergenic 35.0%
CAAGAGTTTAGCATACTTCA+TGG - chr1_1:27870993-27871012 Msa0016740:intron 35.0%
! TTCTGATGAAAGCACATACA+AGG - chr1_1:27871158-27871177 Msa0016740:intron 35.0%
!!! TGTCTGAGGTTTTTAGAGTA+AGG - chr1_1:27871227-27871246 Msa0016740:intron 35.0%
!!! GTCTGAGGTTTTTAGAGTAA+GGG - chr1_1:27871228-27871247 Msa0016740:intron 35.0%
CACTAATGAAGAGAGAACTT+GGG + chr1_1:27871280-27871299 None:intergenic 35.0%
TAAAGATCAGAGACAAGTTG+AGG + chr1_1:27871325-27871344 None:intergenic 35.0%
!! TTAGCTTTTATAGCCTCTGT+TGG - chr1_1:27871392-27871411 Msa0016740:intron 35.0%
! TAGCTTTTATAGCCTCTGTT+GGG - chr1_1:27871393-27871412 Msa0016740:intron 35.0%
GACAAAATGCATCAAAGATC+AGG + chr1_1:27871426-27871445 None:intergenic 35.0%
GTTGTAACTACAGCTTAGAT+AGG + chr1_1:27871478-27871497 None:intergenic 35.0%
! CACATTTGAGACAGAAGTAA+GGG + chr1_1:27871500-27871519 None:intergenic 35.0%
! TCACATTTGAGACAGAAGTA+AGG + chr1_1:27871501-27871520 None:intergenic 35.0%
ACTTCTGTCTCAAATGTGAT+TGG - chr1_1:27871502-27871521 Msa0016740:CDS 35.0%
GGTTTGAGGGTTGTAATTTA+GGG - chr1_1:27871619-27871638 Msa0016740:five_prime_UTR 35.0%
AGGATTGAATGAAGAGTCAA+AGG - chr1_1:27871750-27871769 Msa0016740:intron 35.0%
!! ATCAAAAAGCATCTCAGCAT+AGG + chr1_1:27871776-27871795 None:intergenic 35.0%
!! TGCTTTTTGATACTCTTGCT+CGG - chr1_1:27871785-27871804 Msa0016740:intron 35.0%
CGATTAACAAGATCCAGTTT+AGG - chr1_1:27871830-27871849 Msa0016740:five_prime_UTR 35.0%
ATTCGGCCATGTATATGATA+TGG - chr1_1:27872037-27872056 Msa0016740:five_prime_UTR 35.0%
AAATACCCATGCCACATAAG+GGG + chr1_1:27869353-27869372 None:intergenic 40.0%
GAGATCTAAGAGAGAGAAGA+GGG + chr1_1:27869406-27869425 None:intergenic 40.0%
!! TATGGATGGTTGATTCGATC+TGG - chr1_1:27869746-27869765 Msa0016740:intron 40.0%
GTTCAAAATCGCTGAGAGAT+TGG - chr1_1:27869780-27869799 Msa0016740:intron 40.0%
CATTACCAACACATGAAGAG+GGG + chr1_1:27869806-27869825 None:intergenic 40.0%
CCATTACCAACACATGAAGA+GGG + chr1_1:27869807-27869826 None:intergenic 40.0%
!! CCTCTTCATGTGTTGGTAAT+GGG - chr1_1:27869805-27869824 Msa0016740:intron 40.0%
!! ATCCAGTTTTAGTCATCCTC+AGG - chr1_1:27869925-27869944 Msa0016740:intron 40.0%
GGTTCTTCTTGGTCATTTCA+TGG - chr1_1:27870474-27870493 Msa0016740:CDS 40.0%
GAAGATATCTTATGGATCCG+TGG - chr1_1:27870510-27870529 Msa0016740:intron 40.0%
!! TGCTATTGACTTGCATAGTG+TGG - chr1_1:27870581-27870600 Msa0016740:intron 40.0%
!! GCTTGCTGTTTCAAATGACA+AGG - chr1_1:27870648-27870667 Msa0016740:intron 40.0%
ATACTACAGTGTAATCACGC+AGG + chr1_1:27870751-27870770 None:intergenic 40.0%
CGTGATTACACTGTAGTATG+TGG - chr1_1:27870752-27870771 Msa0016740:intron 40.0%
CTCTTGAAGTTCACATTGTG+TGG + chr1_1:27870979-27870998 None:intergenic 40.0%
GTTACCTGGTAGCATAATGT+AGG + chr1_1:27871024-27871043 None:intergenic 40.0%
!! TTTGGTTTGGAGTATGTCTG+AGG - chr1_1:27871213-27871232 Msa0016740:intron 40.0%
CCACTAATGAAGAGAGAACT+TGG + chr1_1:27871281-27871300 None:intergenic 40.0%
CCAAGTTCTCTCTTCATTAG+TGG - chr1_1:27871278-27871297 Msa0016740:intron 40.0%
! TGTCTATTCTGTCCTCCTTT+TGG - chr1_1:27871361-27871380 Msa0016740:intron 40.0%
AAGCTAAGCAGATGACCAAA+AGG + chr1_1:27871379-27871398 None:intergenic 40.0%
!! GTCTCAAATGTGATTGGTCT+CGG - chr1_1:27871508-27871527 Msa0016740:CDS 40.0%
TCCATCTCTTGTGCTAAATG+TGG - chr1_1:27871554-27871573 Msa0016740:exon 40.0%
ACCACATTTAGCACAAGAGA+TGG + chr1_1:27871558-27871577 None:intergenic 40.0%
TCCTTCAATTTGTGGGTTTG+AGG - chr1_1:27871605-27871624 Msa0016740:five_prime_UTR 40.0%
CCTTCAATTTGTGGGTTTGA+GGG - chr1_1:27871606-27871625 Msa0016740:five_prime_UTR 40.0%
GGGTTTGAGGGTTGTAATTT+AGG - chr1_1:27871618-27871637 Msa0016740:five_prime_UTR 40.0%
! CAGACCAAAGCTTTGATACT+AGG - chr1_1:27871872-27871891 Msa0016740:five_prime_UTR 40.0%
TGAGCCTAGTATCAAAGCTT+TGG + chr1_1:27871879-27871898 None:intergenic 40.0%
TGGAAGAGTGGTATGGAGAA+GGG + chr1_1:27869326-27869345 None:intergenic 45.0%
ATGGAAGAGTGGTATGGAGA+AGG + chr1_1:27869327-27869346 None:intergenic 45.0%
! ATAAGGGGTGTATGGAAGAG+TGG + chr1_1:27869338-27869357 None:intergenic 45.0%
TCTTCCATACACCCCTTATG+TGG - chr1_1:27869339-27869358 Msa0016740:three_prime_UTR 45.0%
ATACACCCCTTATGTGGCAT+GGG - chr1_1:27869345-27869364 Msa0016740:three_prime_UTR 45.0%
GGAGATCTAAGAGAGAGAAG+AGG + chr1_1:27869407-27869426 None:intergenic 45.0%
AGAGTGAGAGGGAGAAGAAA+GGG + chr1_1:27869428-27869447 None:intergenic 45.0%
CACTGTTTAAGGGAGAGAGT+GGG + chr1_1:27869475-27869494 None:intergenic 45.0%
TCACTGTTTAAGGGAGAGAG+TGG + chr1_1:27869476-27869495 None:intergenic 45.0%
! CTGTATTGTGGTGCTTGTTG+CGG - chr1_1:27869568-27869587 Msa0016740:CDS 45.0%
! TGCTTGTTGCGGTGAATAGT+AGG - chr1_1:27869579-27869598 Msa0016740:CDS 45.0%
!!! GGTTGATTCGATCTGGCTTT+TGG - chr1_1:27869753-27869772 Msa0016740:intron 45.0%
! CCCTCTTCATGTGTTGGTAA+TGG - chr1_1:27869804-27869823 Msa0016740:intron 45.0%
CCCATTACCAACACATGAAG+AGG + chr1_1:27869808-27869827 None:intergenic 45.0%
GACCTGAGGATGACTAAAAC+TGG + chr1_1:27869930-27869949 None:intergenic 45.0%
CTTGGTCATTTCATGGGCAT+TGG - chr1_1:27870481-27870500 Msa0016740:CDS 45.0%
GCAAGTCAATAGCATCAACG+TGG + chr1_1:27870575-27870594 None:intergenic 45.0%
AGCAAGCGCTCTCATATCAT+AGG + chr1_1:27870635-27870654 None:intergenic 45.0%
GATTGGAAGTGGAAGCAGAA+TGG + chr1_1:27870807-27870826 None:intergenic 45.0%
CAAGAACAGAGGATTGGAAG+TGG + chr1_1:27870818-27870837 None:intergenic 45.0%
CACTTCCAATCCTCTGTTCT+TGG - chr1_1:27870816-27870835 Msa0016740:CDS 45.0%
AGAAGCCAAGAACAGAGGAT+TGG + chr1_1:27870824-27870843 None:intergenic 45.0%
! ACTGTTTCTCCCTATACCAC+AGG + chr1_1:27870931-27870950 None:intergenic 45.0%
TGCTCCTACATTATGCTACC+AGG - chr1_1:27871017-27871036 Msa0016740:intron 45.0%
!! GTGTCATCGTTGCATCGATT+TGG - chr1_1:27871097-27871116 Msa0016740:intron 45.0%
AAGTTGAGGAAGCAGTCCAT+AGG + chr1_1:27871311-27871330 None:intergenic 45.0%
CTAAGCAGATGACCAAAAGG+AGG + chr1_1:27871376-27871395 None:intergenic 45.0%
AAACTACATCTACCGCAGCT+GGG + chr1_1:27871448-27871467 None:intergenic 45.0%
CCCTCAAACCCACAAATTGA+AGG + chr1_1:27871609-27871628 None:intergenic 45.0%
AGATCCAGTTTAGGGAGTTC+TGG - chr1_1:27871839-27871858 Msa0016740:five_prime_UTR 45.0%
GATCCAGTTTAGGGAGTTCT+GGG - chr1_1:27871840-27871859 Msa0016740:five_prime_UTR 45.0%
GGGTGTATGGAAGAGTGGTA+TGG + chr1_1:27869333-27869352 None:intergenic 50.0%
! CATGCCACATAAGGGGTGTA+TGG + chr1_1:27869346-27869365 None:intergenic 50.0%
CATACACCCCTTATGTGGCA+TGG - chr1_1:27869344-27869363 Msa0016740:three_prime_UTR 50.0%
CAGAGTGAGAGGGAGAAGAA+AGG + chr1_1:27869429-27869448 None:intergenic 50.0%
ACAGTCCTGCATGACTCTGA+CGG + chr1_1:27869541-27869560 None:intergenic 50.0%
ATTGGCCCCTCTTCATGTGT+TGG - chr1_1:27869798-27869817 Msa0016740:intron 50.0%
AGAATCGCACCTGCACAGTA+AGG + chr1_1:27870536-27870555 None:intergenic 50.0%
CAGCAAGAAGCCAAGAACAG+AGG + chr1_1:27870829-27870848 None:intergenic 50.0%
CTCTGTTCTTGGCTTCTTGC+TGG - chr1_1:27870827-27870846 Msa0016740:CDS 50.0%
GTTCTTGGCTTCTTGCTGGA+TGG - chr1_1:27870831-27870850 Msa0016740:CDS 50.0%
TCAGGGTCAAAACCCAACAG+AGG + chr1_1:27871408-27871427 None:intergenic 50.0%
! GATGCATTTTGTCCCAGCTG+CGG - chr1_1:27871433-27871452 Msa0016740:intron 50.0%
CAAACTACATCTACCGCAGC+TGG + chr1_1:27871449-27871468 None:intergenic 50.0%
CAGCTGCGGTAGATGTAGTT+TGG - chr1_1:27871447-27871466 Msa0016740:intron 50.0%
ATACTCTTGCTCGGCAGAGA+GGG - chr1_1:27871794-27871813 Msa0016740:intron 50.0%
AGTCCCAGAACTCCCTAAAC+TGG + chr1_1:27871846-27871865 None:intergenic 50.0%
GAGTGAGAGGCAGAGTGAGA+GGG + chr1_1:27869439-27869458 None:intergenic 55.0%
TGAGTGAGAGGCAGAGTGAG+AGG + chr1_1:27869440-27869459 None:intergenic 55.0%
CTCTGCCTCTCACTCACTTC+CGG - chr1_1:27869444-27869463 Msa0016740:three_prime_UTR 55.0%
AACGACCGGAAGTGAGTGAG+AGG + chr1_1:27869452-27869471 None:intergenic 55.0%
ACTGTGAGAGTGGGAGGAGA+AGG + chr1_1:27869512-27869531 None:intergenic 55.0%
GATACTCTTGCTCGGCAGAG+AGG - chr1_1:27871793-27871812 Msa0016740:intron 55.0%
AGGGAGAGAGTGGGAACGAC+CGG + chr1_1:27869466-27869485 None:intergenic 60.0%
CCTCCCACTCTCACAGTCGT+CGG - chr1_1:27869515-27869534 Msa0016740:exon 60.0%
! GATCCGTGGCCTTACTGTGC+AGG - chr1_1:27870524-27870543 Msa0016740:intron 60.0%
GCACCTGCACAGTAAGGCCA+CGG + chr1_1:27870530-27870549 None:intergenic 60.0%
GCTCGGCAGAGAGGGATTCA+AGG - chr1_1:27871802-27871821 Msa0016740:intron 60.0%
CGGCAGAGAGGGATTCAAGG+AGG - chr1_1:27871805-27871824 Msa0016740:intron 60.0%
GAGAGTGGGAGGAGAAGGCC+AGG + chr1_1:27869507-27869526 None:intergenic 65.0%
CCGACGACTGTGAGAGTGGG+AGG + chr1_1:27869518-27869537 None:intergenic 65.0%
CGGCCGACGACTGTGAGAGT+GGG + chr1_1:27869521-27869540 None:intergenic 65.0%
ACGGCCGACGACTGTGAGAG+TGG + chr1_1:27869522-27869541 None:intergenic 65.0%
GTCGGCCGTCAGAGTCATGC+AGG - chr1_1:27869533-27869552 Msa0016740:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 27869305 27872135 27869305 ID=Msa0016740;Name=Msa0016740
chr1_1 mRNA 27869305 27872135 27869305 ID=Msa0016740-mRNA-1;Parent=Msa0016740;Name=Msa0016740-mRNA-1;_AED=0.18;_eAED=0.18;_QI=445|0.6|0.83|0.83|0.8|0.83|6|217|191
chr1_1 exon 27869305 27869688 27869305 ID=Msa0016740-mRNA-1:exon:9367;Parent=Msa0016740-mRNA-1
chr1_1 exon 27869986 27870094 27869986 ID=Msa0016740-mRNA-1:exon:9366;Parent=Msa0016740-mRNA-1
chr1_1 exon 27870402 27870500 27870402 ID=Msa0016740-mRNA-1:exon:9365;Parent=Msa0016740-mRNA-1
chr1_1 exon 27870771 27870894 27870771 ID=Msa0016740-mRNA-1:exon:9364;Parent=Msa0016740-mRNA-1
chr1_1 exon 27871494 27871694 27871494 ID=Msa0016740-mRNA-1:exon:9363;Parent=Msa0016740-mRNA-1
chr1_1 exon 27871815 27872135 27871815 ID=Msa0016740-mRNA-1:exon:9362;Parent=Msa0016740-mRNA-1
chr1_1 five_prime_UTR 27871815 27872135 27871815 ID=Msa0016740-mRNA-1:five_prime_utr;Parent=Msa0016740-mRNA-1
chr1_1 five_prime_UTR 27871571 27871694 27871571 ID=Msa0016740-mRNA-1:five_prime_utr;Parent=Msa0016740-mRNA-1
chr1_1 CDS 27871494 27871570 27871494 ID=Msa0016740-mRNA-1:cds;Parent=Msa0016740-mRNA-1
chr1_1 CDS 27870771 27870894 27870771 ID=Msa0016740-mRNA-1:cds;Parent=Msa0016740-mRNA-1
chr1_1 CDS 27870402 27870500 27870402 ID=Msa0016740-mRNA-1:cds;Parent=Msa0016740-mRNA-1
chr1_1 CDS 27869986 27870094 27869986 ID=Msa0016740-mRNA-1:cds;Parent=Msa0016740-mRNA-1
chr1_1 CDS 27869522 27869688 27869522 ID=Msa0016740-mRNA-1:cds;Parent=Msa0016740-mRNA-1
chr1_1 three_prime_UTR 27869305 27869521 27869305 ID=Msa0016740-mRNA-1:three_prime_utr;Parent=Msa0016740-mRNA-1
Gene Sequence

>Msa0016740

ATGTTGCAGGTTGTTGTTCTACTTCATATTGTAATTACAACTGTTGCAATTATCTATCCAGTTTTAGTCATCCTCAGGTGCGATTCTGCTTTTTTATCTGGTTCCACGTTGATGCTATTGACTTGCATAGTGTGGTTAAAATTGGTGTCATATGCACATACAACCTATGATATGAGAGCGCTTGCTGTTTCAAATGACAAGGGAGAAACAGTGCCTGATACTTTGAATATGGAGTACCCACACAATGTGAACTTCAAGAGTTTAGCATACTTCATGGTTGCTCCTACATTATGCTACCAGATCAATTTGTCTAACTGTCTATTCTGTCCTCCTTTTGGTCATCTGCTTAGCTTTTATAGCCTCTGTTGGGTTTTGACCCTGATCTTTGATGCATTTTGTCCCAGCTGCGGATTGAATGAAGAGTCAAAGGCCTATGCTGAGATGCTTTTTGATACTCTTGCTCGGCAGAGAGGGATTCAAGGAGGTTCGATTAACAAGATCCAGTTTAGGGAGTTCTGGGACTGTATTTCAGACCAAAGCTTTGATACTAGGCTCAAAATATTCTTTGACATGTAA
Protein sequence

>Msa0016740

MLQVVVLLHIVITTVAIIYPVLVILRCDSAFLSGSTLMLLTCIVWLKLVSYAHTTYDMRALAVSNDKGETVPDTLNMEYPHNVNFKSLAYFMVAPTLCYQINLSNCLFCPPFGHLLSFYSLCWVLTLIFDAFCPSCGLNEESKAYAEMLFDTLARQRGIQGGSINKIQFREFWDCISDQSFDTRLKIFFDM*