Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026440 | Msa0145250 | 0.805892 | 1.071701e-49 | -8.615850e-47 |
| Msa0026440 | Msa0165510 | 0.874400 | 6.955908e-68 | -8.615850e-47 |
| Msa0026440 | Msa1043830 | 0.816323 | 5.868275e-52 | -8.615850e-47 |
| Msa0026440 | Msa1453250 | 0.866309 | 3.132485e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0026440 | MtrunA17_Chr1g0153611 | 95.699 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 3.10e-63 | 186 |
| Msa0026440 | MtrunA17_Chr1g0153581 | 68.085 | 94 | 29 | 1 | 1 | 93 | 1 | 94 | 3.35e-40 | 128 |
| Msa0026440 | MtrunA17_Chr1g0153561 | 67.021 | 94 | 30 | 1 | 1 | 93 | 1 | 94 | 1.56e-39 | 126 |
| Msa0026440 | MtrunA17_Chr1g0153601 | 65.957 | 94 | 31 | 1 | 1 | 93 | 1 | 94 | 1.04e-38 | 124 |
| Msa0026440 | MtrunA17_Chr1g0153551 | 45.556 | 90 | 47 | 2 | 1 | 88 | 5 | 94 | 2.88e-20 | 77.8 |
| Msa0026440 | MtrunA17_Chr1g0153541 | 42.391 | 92 | 48 | 2 | 1 | 87 | 5 | 96 | 1.60e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATTGTCTTCTCTCTATC+AGG | 0.235282 | 1_1:+50301295 | None:intergenic |
| TATCTTCTTGTCAAAGGGTT+TGG | 0.309945 | 1_1:-50301058 | Msa0026440:CDS |
| GATTTACGTTCCTATGTTCC+TGG | 0.332407 | 1_1:-50301166 | Msa0026440:CDS |
| CAAAGCAGAAAGGAAAATAA+TGG | 0.385640 | 1_1:-50301260 | Msa0026440:CDS |
| ACTAATTTGTCCATCATTGT+TGG | 0.414841 | 1_1:+50301108 | None:intergenic |
| AAGGAAAATAATGGAAGTAT+TGG | 0.434553 | 1_1:-50301251 | Msa0026440:CDS |
| TCTCTATCAGGGCGTAGTAT+AGG | 0.452488 | 1_1:+50301307 | None:intergenic |
| TTGATTATCTTCTTGTCAAA+GGG | 0.453582 | 1_1:-50301063 | Msa0026440:CDS |
| GTTGATTATCTTCTTGTCAA+AGG | 0.460253 | 1_1:-50301064 | Msa0026440:CDS |
| AATATTGATGCCAACAATGA+TGG | 0.465249 | 1_1:-50301118 | Msa0026440:CDS |
| CAACGCATGGCTCTCCATCC+AGG | 0.488249 | 1_1:+50301148 | None:intergenic |
| TACGTTCCTATGTTCCTGGA+TGG | 0.524072 | 1_1:-50301162 | Msa0026440:CDS |
| ATGGAGAGCCATGCGTTGTA+TGG | 0.529428 | 1_1:-50301143 | Msa0026440:CDS |
| AACAATGATGGACAAATTAG+TGG | 0.554056 | 1_1:-50301106 | Msa0026440:CDS |
| AAGATCAAATCAAAGCAGAA+AGG | 0.570009 | 1_1:-50301270 | Msa0026440:CDS |
| GGCTCTCCATCCAGGAACAT+AGG | 0.575847 | 1_1:+50301156 | None:intergenic |
| AATATTGACCATACAACGCA+TGG | 0.602870 | 1_1:+50301135 | None:intergenic |
| AAATTGTCTTCTCTCTATCA+GGG | 0.603477 | 1_1:+50301296 | None:intergenic |
| TGATGGACAAATTAGTGGTG+AGG | 0.626914 | 1_1:-50301101 | Msa0026440:CDS |
| AAAGCAGATAGCAATAAGGA+CGG | 0.681326 | 1_1:-50301226 | Msa0026440:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGAAAATAATGGAAGTAT+TGG | - | chr1_1:50301110-50301129 | Msa0026440:CDS | 25.0% |
| ! | TTGATTATCTTCTTGTCAAA+GGG | - | chr1_1:50301298-50301317 | Msa0026440:CDS | 25.0% |
| AAATTGTCTTCTCTCTATCA+GGG | + | chr1_1:50301068-50301087 | None:intergenic | 30.0% | |
| AAGATCAAATCAAAGCAGAA+AGG | - | chr1_1:50301091-50301110 | Msa0026440:CDS | 30.0% | |
| CAAAGCAGAAAGGAAAATAA+TGG | - | chr1_1:50301101-50301120 | Msa0026440:CDS | 30.0% | |
| AATATTGATGCCAACAATGA+TGG | - | chr1_1:50301243-50301262 | Msa0026440:CDS | 30.0% | |
| ! | ACTAATTTGTCCATCATTGT+TGG | + | chr1_1:50301256-50301275 | None:intergenic | 30.0% |
| !! | AACAATGATGGACAAATTAG+TGG | - | chr1_1:50301255-50301274 | Msa0026440:CDS | 30.0% |
| GTTGATTATCTTCTTGTCAA+AGG | - | chr1_1:50301297-50301316 | Msa0026440:CDS | 30.0% | |
| GAAATTGTCTTCTCTCTATC+AGG | + | chr1_1:50301069-50301088 | None:intergenic | 35.0% | |
| GGAAAAAGCAGATAGCAATA+AGG | - | chr1_1:50301131-50301150 | Msa0026440:CDS | 35.0% | |
| AAAGCAGATAGCAATAAGGA+CGG | - | chr1_1:50301135-50301154 | Msa0026440:CDS | 35.0% | |
| AATATTGACCATACAACGCA+TGG | + | chr1_1:50301229-50301248 | None:intergenic | 35.0% | |
| TATCTTCTTGTCAAAGGGTT+TGG | - | chr1_1:50301303-50301322 | Msa0026440:CDS | 35.0% | |
| GATTTACGTTCCTATGTTCC+TGG | - | chr1_1:50301195-50301214 | Msa0026440:CDS | 40.0% | |
| ! | TGATGGACAAATTAGTGGTG+AGG | - | chr1_1:50301260-50301279 | Msa0026440:CDS | 40.0% |
| TCTCTATCAGGGCGTAGTAT+AGG | + | chr1_1:50301057-50301076 | None:intergenic | 45.0% | |
| TACGTTCCTATGTTCCTGGA+TGG | - | chr1_1:50301199-50301218 | Msa0026440:CDS | 45.0% | |
| ATGGAGAGCCATGCGTTGTA+TGG | - | chr1_1:50301218-50301237 | Msa0026440:CDS | 50.0% | |
| GGCTCTCCATCCAGGAACAT+AGG | + | chr1_1:50301208-50301227 | None:intergenic | 55.0% | |
| CAACGCATGGCTCTCCATCC+AGG | + | chr1_1:50301216-50301235 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 50301051 | 50301332 | 50301051 | ID=Msa0026440;Name=Msa0026440 |
| chr1_1 | mRNA | 50301051 | 50301332 | 50301051 | ID=Msa0026440-mRNA-1;Parent=Msa0026440;Name=Msa0026440-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|93 |
| chr1_1 | exon | 50301051 | 50301332 | 50301051 | ID=Msa0026440-mRNA-1:exon:14609;Parent=Msa0026440-mRNA-1 |
| chr1_1 | CDS | 50301051 | 50301332 | 50301051 | ID=Msa0026440-mRNA-1:cds;Parent=Msa0026440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
