AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453250 MtrunA17_Chr1g0153611 96.774 93 3 0 1 93 1 93 8.68e-64 187
Msa1453250 MtrunA17_Chr1g0153581 68.085 94 29 1 1 93 1 94 3.99e-40 127
Msa1453250 MtrunA17_Chr1g0153561 67.021 94 30 1 1 93 1 94 1.27e-39 126
Msa1453250 MtrunA17_Chr1g0153601 65.957 94 31 1 1 93 1 94 1.02e-38 124
Msa1453250 MtrunA17_Chr1g0153551 46.667 90 46 2 1 88 5 94 1.14e-20 79.0
Msa1453250 MtrunA17_Chr1g0153541 42.391 92 48 2 1 87 5 96 1.57e-17 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTGTCTTCTTTCTATC+AGG 0.222096 tig0034550:-1344 None:intergenic
TATCTTCTTGTCAAAGGGTT+TGG 0.309945 tig0034550:+1581 Msa1453250:CDS
GATTTACGTTCCTATGTTCC+TGG 0.332407 tig0034550:+1473 Msa1453250:CDS
CAAAGCAGAAAGGAAAATAA+TGG 0.385640 tig0034550:+1379 Msa1453250:CDS
ACTAATTTGTCCATCATTGT+TGG 0.414841 tig0034550:-1531 None:intergenic
TTTCTATCAGGGCGTAGTAT+AGG 0.421580 tig0034550:-1332 None:intergenic
AAGGAAAATAATGGAAGTAT+TGG 0.434553 tig0034550:+1388 Msa1453250:CDS
TTGATTATCTTCTTGTCAAA+GGG 0.453582 tig0034550:+1576 Msa1453250:CDS
GTTGATTATCTTCTTGTCAA+AGG 0.460253 tig0034550:+1575 Msa1453250:CDS
AATATTGATGCCAACAATGA+TGG 0.465249 tig0034550:+1521 Msa1453250:CDS
CAACGCATGGCTCTCCATCC+AGG 0.488249 tig0034550:-1491 None:intergenic
TACGTTCCTATGTTCCTGGA+TGG 0.524072 tig0034550:+1477 Msa1453250:CDS
AAATTGTCTTCTTTCTATCA+GGG 0.524225 tig0034550:-1343 None:intergenic
ATGGAGAGCCATGCGTTGTA+TGG 0.529428 tig0034550:+1496 Msa1453250:CDS
AACAATGATGGACAAATTAG+TGG 0.554056 tig0034550:+1533 Msa1453250:CDS
AAGATCAAATCAAAGCAGAA+AGG 0.570009 tig0034550:+1369 Msa1453250:CDS
GGCTCTCCATCCAGGAACAT+AGG 0.575847 tig0034550:-1483 None:intergenic
AATATTGACCATACAACGCA+TGG 0.602870 tig0034550:-1504 None:intergenic
TGATGGACAAATTAGTGGTG+AGG 0.626914 tig0034550:+1538 Msa1453250:CDS
AAAGCAGATAGCAATAAGGA+CGG 0.681326 tig0034550:+1413 Msa1453250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATTGTCTTCTTTCTATCA+GGG - tig0034550:1346-1365 None:intergenic 25.0%
!! AAGGAAAATAATGGAAGTAT+TGG + tig0034550:1388-1407 Msa1453250:CDS 25.0%
! TTGATTATCTTCTTGTCAAA+GGG + tig0034550:1576-1595 Msa1453250:CDS 25.0%
GAAATTGTCTTCTTTCTATC+AGG - tig0034550:1347-1366 None:intergenic 30.0%
AAGATCAAATCAAAGCAGAA+AGG + tig0034550:1369-1388 Msa1453250:CDS 30.0%
CAAAGCAGAAAGGAAAATAA+TGG + tig0034550:1379-1398 Msa1453250:CDS 30.0%
AATATTGATGCCAACAATGA+TGG + tig0034550:1521-1540 Msa1453250:CDS 30.0%
! ACTAATTTGTCCATCATTGT+TGG - tig0034550:1534-1553 None:intergenic 30.0%
!! AACAATGATGGACAAATTAG+TGG + tig0034550:1533-1552 Msa1453250:CDS 30.0%
GTTGATTATCTTCTTGTCAA+AGG + tig0034550:1575-1594 Msa1453250:CDS 30.0%
GGAAAAAGCAGATAGCAATA+AGG + tig0034550:1409-1428 Msa1453250:CDS 35.0%
AAAGCAGATAGCAATAAGGA+CGG + tig0034550:1413-1432 Msa1453250:CDS 35.0%
AATATTGACCATACAACGCA+TGG - tig0034550:1507-1526 None:intergenic 35.0%
TATCTTCTTGTCAAAGGGTT+TGG + tig0034550:1581-1600 Msa1453250:CDS 35.0%
TTTCTATCAGGGCGTAGTAT+AGG - tig0034550:1335-1354 None:intergenic 40.0%
GATTTACGTTCCTATGTTCC+TGG + tig0034550:1473-1492 Msa1453250:CDS 40.0%
! TGATGGACAAATTAGTGGTG+AGG + tig0034550:1538-1557 Msa1453250:CDS 40.0%
TACGTTCCTATGTTCCTGGA+TGG + tig0034550:1477-1496 Msa1453250:CDS 45.0%
ATGGAGAGCCATGCGTTGTA+TGG + tig0034550:1496-1515 Msa1453250:CDS 50.0%
GGCTCTCCATCCAGGAACAT+AGG - tig0034550:1486-1505 None:intergenic 55.0%
CAACGCATGGCTCTCCATCC+AGG - tig0034550:1494-1513 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0034550 gene 1329 1610 1329 ID=Msa1453250;Name=Msa1453250
tig0034550 mRNA 1329 1610 1329 ID=Msa1453250-mRNA-1;Parent=Msa1453250;Name=Msa1453250-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|93
tig0034550 exon 1329 1610 1329 ID=Msa1453250-mRNA-1:exon:19062;Parent=Msa1453250-mRNA-1
tig0034550 CDS 1329 1610 1329 ID=Msa1453250-mRNA-1:cds;Parent=Msa1453250-mRNA-1
Gene Sequence

>Msa1453250

ATGCCTATACTACGCCCTGATAGAAAGAAGACAATTTCTCAAGATCAAATCAAAGCAGAAAGGAAAATAATGGAAGTATTGGAAAAAGCAGATAGCAATAAGGACGGTTGTTATACAAAGAGTGAGATCAAGAAAGCTCTCAAAGATTTACGTTCCTATGTTCCTGGATGGAGAGCCATGCGTTGTATGGTCAATATTGATGCCAACAATGATGGACAAATTAGTGGTGAGGAAATTGATACTTTAGTTGATTATCTTCTTGTCAAAGGGTTTGGAAAGTAA

Protein sequence

>Msa1453250

MPILRPDRKKTISQDQIKAERKIMEVLEKADSNKDGCYTKSEIKKALKDLRSYVPGWRAMRCMVNIDANNDGQISGEEIDTLVDYLLVKGFGK*