AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0165510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165510 MtrunA17_Chr1g0153611 96.774 93 3 0 1 93 1 93 8.68e-64 187
Msa0165510 MtrunA17_Chr1g0153581 68.085 94 29 1 1 93 1 94 3.99e-40 127
Msa0165510 MtrunA17_Chr1g0153561 67.021 94 30 1 1 93 1 94 1.27e-39 126
Msa0165510 MtrunA17_Chr1g0153601 65.957 94 31 1 1 93 1 94 1.02e-38 124
Msa0165510 MtrunA17_Chr1g0153551 46.667 90 46 2 1 88 5 94 1.14e-20 79.0
Msa0165510 MtrunA17_Chr1g0153541 42.391 92 48 2 1 87 5 96 1.57e-17 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTGTCTTCTTTCTATC+AGG 0.222096 1_4:+46134292 None:intergenic
TATCTTCTTGTCAAAGGGTT+TGG 0.309945 1_4:-46134055 Msa0165510:CDS
GATTTACGTTCCTATGTTCC+TGG 0.332407 1_4:-46134163 Msa0165510:CDS
CAAAGCAGAAAGGAAAATAA+TGG 0.385640 1_4:-46134257 Msa0165510:CDS
ACTAATTTGTCCATCATTGT+TGG 0.414841 1_4:+46134105 None:intergenic
TTTCTATCAGGGCGTAGTAT+AGG 0.421580 1_4:+46134304 None:intergenic
AAGGAAAATAATGGAAGTAT+TGG 0.434553 1_4:-46134248 Msa0165510:CDS
TTGATTATCTTCTTGTCAAA+GGG 0.453582 1_4:-46134060 Msa0165510:CDS
GTTGATTATCTTCTTGTCAA+AGG 0.460253 1_4:-46134061 Msa0165510:CDS
AATATTGATGCCAACAATGA+TGG 0.465249 1_4:-46134115 Msa0165510:CDS
CAACGCATGGCTCTCCATCC+AGG 0.488249 1_4:+46134145 None:intergenic
TACGTTCCTATGTTCCTGGA+TGG 0.524072 1_4:-46134159 Msa0165510:CDS
AAATTGTCTTCTTTCTATCA+GGG 0.524225 1_4:+46134293 None:intergenic
ATGGAGAGCCATGCGTTGTA+TGG 0.529428 1_4:-46134140 Msa0165510:CDS
AACAATGATGGACAAATTAG+TGG 0.554056 1_4:-46134103 Msa0165510:CDS
AAGATCAAATCAAAGCAGAA+AGG 0.570009 1_4:-46134267 Msa0165510:CDS
GGCTCTCCATCCAGGAACAT+AGG 0.575847 1_4:+46134153 None:intergenic
AATATTGACCATACAACGCA+TGG 0.602870 1_4:+46134132 None:intergenic
TGATGGACAAATTAGTGGTG+AGG 0.626914 1_4:-46134098 Msa0165510:CDS
AAAGCAGATAGCAATAAGGA+CGG 0.681326 1_4:-46134223 Msa0165510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATTGTCTTCTTTCTATCA+GGG + chr1_4:46134065-46134084 None:intergenic 25.0%
!! AAGGAAAATAATGGAAGTAT+TGG - chr1_4:46134107-46134126 Msa0165510:CDS 25.0%
! TTGATTATCTTCTTGTCAAA+GGG - chr1_4:46134295-46134314 Msa0165510:CDS 25.0%
GAAATTGTCTTCTTTCTATC+AGG + chr1_4:46134066-46134085 None:intergenic 30.0%
AAGATCAAATCAAAGCAGAA+AGG - chr1_4:46134088-46134107 Msa0165510:CDS 30.0%
CAAAGCAGAAAGGAAAATAA+TGG - chr1_4:46134098-46134117 Msa0165510:CDS 30.0%
AATATTGATGCCAACAATGA+TGG - chr1_4:46134240-46134259 Msa0165510:CDS 30.0%
! ACTAATTTGTCCATCATTGT+TGG + chr1_4:46134253-46134272 None:intergenic 30.0%
!! AACAATGATGGACAAATTAG+TGG - chr1_4:46134252-46134271 Msa0165510:CDS 30.0%
GTTGATTATCTTCTTGTCAA+AGG - chr1_4:46134294-46134313 Msa0165510:CDS 30.0%
GGAAAAAGCAGATAGCAATA+AGG - chr1_4:46134128-46134147 Msa0165510:CDS 35.0%
AAAGCAGATAGCAATAAGGA+CGG - chr1_4:46134132-46134151 Msa0165510:CDS 35.0%
AATATTGACCATACAACGCA+TGG + chr1_4:46134226-46134245 None:intergenic 35.0%
TATCTTCTTGTCAAAGGGTT+TGG - chr1_4:46134300-46134319 Msa0165510:CDS 35.0%
TTTCTATCAGGGCGTAGTAT+AGG + chr1_4:46134054-46134073 None:intergenic 40.0%
GATTTACGTTCCTATGTTCC+TGG - chr1_4:46134192-46134211 Msa0165510:CDS 40.0%
! TGATGGACAAATTAGTGGTG+AGG - chr1_4:46134257-46134276 Msa0165510:CDS 40.0%
TACGTTCCTATGTTCCTGGA+TGG - chr1_4:46134196-46134215 Msa0165510:CDS 45.0%
ATGGAGAGCCATGCGTTGTA+TGG - chr1_4:46134215-46134234 Msa0165510:CDS 50.0%
GGCTCTCCATCCAGGAACAT+AGG + chr1_4:46134205-46134224 None:intergenic 55.0%
CAACGCATGGCTCTCCATCC+AGG + chr1_4:46134213-46134232 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 46134048 46134329 46134048 ID=Msa0165510;Name=Msa0165510
chr1_4 mRNA 46134048 46134329 46134048 ID=Msa0165510-mRNA-1;Parent=Msa0165510;Name=Msa0165510-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|93
chr1_4 exon 46134048 46134329 46134048 ID=Msa0165510-mRNA-1:exon:13242;Parent=Msa0165510-mRNA-1
chr1_4 CDS 46134048 46134329 46134048 ID=Msa0165510-mRNA-1:cds;Parent=Msa0165510-mRNA-1
Gene Sequence

>Msa0165510

ATGCCTATACTACGCCCTGATAGAAAGAAGACAATTTCTCAAGATCAAATCAAAGCAGAAAGGAAAATAATGGAAGTATTGGAAAAAGCAGATAGCAATAAGGACGGTTGTTATACAAAGAGTGAGATCAAGAAAGCTCTCAAAGATTTACGTTCCTATGTTCCTGGATGGAGAGCCATGCGTTGTATGGTCAATATTGATGCCAACAATGATGGACAAATTAGTGGTGAGGAAATTGATACTTTAGTTGATTATCTTCTTGTCAAAGGGTTTGGAAAGTAA

Protein sequence

>Msa0165510

MPILRPDRKKTISQDQIKAERKIMEVLEKADSNKDGCYTKSEIKKALKDLRSYVPGWRAMRCMVNIDANNDGQISGEEIDTLVDYLLVKGFGK*