Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043830 | AES59462.1 | 95.699 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 3.37e-59 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043830 | G7I3M7 | 95.699 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 1.61e-59 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007080 | Msa1043830 | 0.815784 | 7.740234e-52 | -8.615850e-47 |
| Msa0017510 | Msa1043830 | 0.828784 | 7.416651e-55 | -8.615850e-47 |
| Msa0026440 | Msa1043830 | 0.816323 | 5.868275e-52 | -8.615850e-47 |
| Msa0100090 | Msa1043830 | 0.800797 | 1.217644e-48 | -8.615850e-47 |
| Msa0112750 | Msa1043830 | 0.806527 | 7.876238e-50 | -8.615850e-47 |
| Msa0626280 | Msa1043830 | 0.803317 | 3.692371e-49 | -8.615850e-47 |
| Msa0641360 | Msa1043830 | 0.800317 | 1.525188e-48 | -8.615850e-47 |
| Msa0658570 | Msa1043830 | 0.803706 | 3.067261e-49 | -8.615850e-47 |
| Msa0682550 | Msa1043830 | 0.817068 | 3.993469e-52 | -8.615850e-47 |
| Msa0693380 | Msa1043830 | 0.809356 | 1.970221e-50 | -8.615850e-47 |
| Msa0727710 | Msa1043830 | 0.814623 | 1.402268e-51 | -8.615850e-47 |
| Msa0766630 | Msa1043830 | 0.801231 | 9.929159e-49 | -8.615850e-47 |
| Msa0777930 | Msa1043830 | 0.830913 | 2.246746e-55 | -8.615850e-47 |
| Msa0145250 | Msa1043830 | 0.851249 | 1.003432e-60 | -8.615850e-47 |
| Msa0157290 | Msa1043830 | 0.828055 | 1.112002e-54 | -8.615850e-47 |
| Msa0165510 | Msa1043830 | 0.872404 | 3.264716e-67 | -8.615850e-47 |
| Msa0173990 | Msa1043830 | 0.814286 | 1.664729e-51 | -8.615850e-47 |
| Msa0197660 | Msa1043830 | 0.805346 | 1.395186e-49 | -8.615850e-47 |
| Msa0227470 | Msa1043830 | 0.803719 | 3.047616e-49 | -8.615850e-47 |
| Msa0419980 | Msa1043830 | 0.815158 | 1.066750e-51 | -8.615850e-47 |
| Msa0437560 | Msa1043830 | 0.800181 | 1.625927e-48 | -8.615850e-47 |
| Msa0452920 | Msa1043830 | 0.809123 | 2.210723e-50 | -8.615850e-47 |
| Msa1043830 | Msa1082500 | 0.823126 | 1.637869e-53 | -8.615850e-47 |
| Msa1043830 | Msa1154070 | 0.801769 | 7.701903e-49 | -8.615850e-47 |
| Msa1043830 | Msa1242220 | 0.810564 | 1.082425e-50 | -8.615850e-47 |
| Msa1043830 | Msa1249490 | 0.800140 | 1.656906e-48 | -8.615850e-47 |
| Msa1043830 | Msa1283860 | 0.807476 | 4.962057e-50 | -8.615850e-47 |
| Msa1043830 | Msa1289280 | 0.809479 | 1.853956e-50 | -8.615850e-47 |
| Msa1043830 | Msa1298260 | 0.807322 | 5.348157e-50 | -8.615850e-47 |
| Msa1043830 | Msa1322890 | 0.818327 | 2.076541e-52 | -8.615850e-47 |
| Msa1043830 | Msa1324780 | 0.818278 | 2.130622e-52 | -8.615850e-47 |
| Msa1043830 | Msa1376890 | 0.800414 | 1.457512e-48 | -8.615850e-47 |
| Msa1043830 | Msa1424680 | 0.815797 | 7.691068e-52 | -8.615850e-47 |
| Msa1043830 | Msa1438250 | 0.813507 | 2.471417e-51 | -8.615850e-47 |
| Msa1043830 | Msa1453250 | 0.871708 | 5.563119e-67 | -8.615850e-47 |
| Msa0245880 | Msa1043830 | 0.801687 | 8.006459e-49 | -8.615850e-47 |
| Msa0285450 | Msa1043830 | 0.802621 | 5.142806e-49 | -8.615850e-47 |
| Msa0317510 | Msa1043830 | 0.800589 | 1.342547e-48 | -8.615850e-47 |
| Msa0340450 | Msa1043830 | 0.804567 | 2.030829e-49 | -8.615850e-47 |
| Msa0484780 | Msa1043830 | 0.817465 | 3.251936e-52 | -8.615850e-47 |
| Msa0503580 | Msa1043830 | 0.800096 | 1.691386e-48 | -8.615850e-47 |
| Msa0512900 | Msa1043830 | 0.810028 | 1.412726e-50 | -8.615850e-47 |
| Msa0558690 | Msa1043830 | 0.812245 | 4.672341e-51 | -8.615850e-47 |
| Msa0843400 | Msa1043830 | 0.803791 | 2.945069e-49 | -8.615850e-47 |
| Msa0867890 | Msa1043830 | 0.801847 | 7.421898e-49 | -8.615850e-47 |
| Msa0880140 | Msa1043830 | 0.828713 | 7.714063e-55 | -8.615850e-47 |
| Msa0909120 | Msa1043830 | 0.804432 | 2.166286e-49 | -8.615850e-47 |
| Msa0935910 | Msa1043830 | 0.820523 | 6.559115e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043830 | MtrunA17_Chr1g0153611 | 95.699 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 3.10e-63 | 186 |
| Msa1043830 | MtrunA17_Chr1g0153581 | 68.085 | 94 | 29 | 1 | 1 | 93 | 1 | 94 | 3.35e-40 | 128 |
| Msa1043830 | MtrunA17_Chr1g0153561 | 67.021 | 94 | 30 | 1 | 1 | 93 | 1 | 94 | 1.56e-39 | 126 |
| Msa1043830 | MtrunA17_Chr1g0153601 | 65.957 | 94 | 31 | 1 | 1 | 93 | 1 | 94 | 1.04e-38 | 124 |
| Msa1043830 | MtrunA17_Chr1g0153551 | 45.556 | 90 | 47 | 2 | 1 | 88 | 5 | 94 | 2.88e-20 | 77.8 |
| Msa1043830 | MtrunA17_Chr1g0153541 | 42.391 | 92 | 48 | 2 | 1 | 87 | 5 | 96 | 1.60e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATTGTCTTCTCTCTATC+AGG | 0.235282 | 7_2:-1974975 | None:intergenic |
| TATCTTCTTGTCAAAGGGTT+TGG | 0.309945 | 7_2:+1975212 | Msa1043830:CDS |
| GATTTACGTTCCTATGTTCC+TGG | 0.332407 | 7_2:+1975104 | Msa1043830:CDS |
| ACTAATTTGTCCATCATTGT+TGG | 0.437941 | 7_2:-1975162 | None:intergenic |
| TCTCTATCAGGGCGTAGTAT+AGG | 0.452488 | 7_2:-1974963 | None:intergenic |
| TTGATTATCTTCTTGTCAAA+GGG | 0.453582 | 7_2:+1975207 | Msa1043830:CDS |
| GTTGATTATCTTCTTGTCAA+AGG | 0.460253 | 7_2:+1975206 | Msa1043830:CDS |
| AATATTGATGCCAACAATGA+TGG | 0.465249 | 7_2:+1975152 | Msa1043830:CDS |
| CAACGCATGGCTCTCCATCC+AGG | 0.488249 | 7_2:-1975122 | None:intergenic |
| AACAATGATGGACAAATTAG+TGG | 0.498216 | 7_2:+1975164 | Msa1043830:CDS |
| TACGTTCCTATGTTCCTGGA+TGG | 0.524072 | 7_2:+1975108 | Msa1043830:CDS |
| ATGGAGAGCCATGCGTTGTA+TGG | 0.529428 | 7_2:+1975127 | Msa1043830:CDS |
| GGCTCTCCATCCAGGAACAT+AGG | 0.575847 | 7_2:-1975114 | None:intergenic |
| AATATTGACCATACAACGCA+TGG | 0.602870 | 7_2:-1975135 | None:intergenic |
| AAATTGTCTTCTCTCTATCA+GGG | 0.603477 | 7_2:-1974974 | None:intergenic |
| TGATGGACAAATTAGTGGCG+AGG | 0.646604 | 7_2:+1975169 | Msa1043830:CDS |
| AAAGCAGATAGCAATAAGGA+CGG | 0.681326 | 7_2:+1975044 | Msa1043830:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGAAAAATAATGGAAGTAT+TGG | + | chr7_2:1975019-1975038 | Msa1043830:CDS | 20.0% |
| ! | CAAAGCAGAAAGAAAAATAA+TGG | + | chr7_2:1975010-1975029 | Msa1043830:CDS | 25.0% |
| ! | TTGATTATCTTCTTGTCAAA+GGG | + | chr7_2:1975207-1975226 | Msa1043830:CDS | 25.0% |
| AAATTGTCTTCTCTCTATCA+GGG | - | chr7_2:1974977-1974996 | None:intergenic | 30.0% | |
| AATATTGATGCCAACAATGA+TGG | + | chr7_2:1975152-1975171 | Msa1043830:CDS | 30.0% | |
| ! | ACTAATTTGTCCATCATTGT+TGG | - | chr7_2:1975165-1975184 | None:intergenic | 30.0% |
| !! | AACAATGATGGACAAATTAG+TGG | + | chr7_2:1975164-1975183 | Msa1043830:CDS | 30.0% |
| GTTGATTATCTTCTTGTCAA+AGG | + | chr7_2:1975206-1975225 | Msa1043830:CDS | 30.0% | |
| GAAATTGTCTTCTCTCTATC+AGG | - | chr7_2:1974978-1974997 | None:intergenic | 35.0% | |
| GGAAAAAGCAGATAGCAATA+AGG | + | chr7_2:1975040-1975059 | Msa1043830:CDS | 35.0% | |
| AAAGCAGATAGCAATAAGGA+CGG | + | chr7_2:1975044-1975063 | Msa1043830:CDS | 35.0% | |
| AATATTGACCATACAACGCA+TGG | - | chr7_2:1975138-1975157 | None:intergenic | 35.0% | |
| TATCTTCTTGTCAAAGGGTT+TGG | + | chr7_2:1975212-1975231 | Msa1043830:CDS | 35.0% | |
| GATTTACGTTCCTATGTTCC+TGG | + | chr7_2:1975104-1975123 | Msa1043830:CDS | 40.0% | |
| TCTCTATCAGGGCGTAGTAT+AGG | - | chr7_2:1974966-1974985 | None:intergenic | 45.0% | |
| TACGTTCCTATGTTCCTGGA+TGG | + | chr7_2:1975108-1975127 | Msa1043830:CDS | 45.0% | |
| ! | TGATGGACAAATTAGTGGCG+AGG | + | chr7_2:1975169-1975188 | Msa1043830:CDS | 45.0% |
| ATGGAGAGCCATGCGTTGTA+TGG | + | chr7_2:1975127-1975146 | Msa1043830:CDS | 50.0% | |
| GGCTCTCCATCCAGGAACAT+AGG | - | chr7_2:1975117-1975136 | None:intergenic | 55.0% | |
| CAACGCATGGCTCTCCATCC+AGG | - | chr7_2:1975125-1975144 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1974960 | 1975241 | 1974960 | ID=Msa1043830;Name=Msa1043830 |
| chr7_2 | mRNA | 1974960 | 1975241 | 1974960 | ID=Msa1043830-mRNA-1;Parent=Msa1043830;Name=Msa1043830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|93 |
| chr7_2 | exon | 1974960 | 1975241 | 1974960 | ID=Msa1043830-mRNA-1:exon:394;Parent=Msa1043830-mRNA-1 |
| chr7_2 | CDS | 1974960 | 1975241 | 1974960 | ID=Msa1043830-mRNA-1:cds;Parent=Msa1043830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
