AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0030270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0030270 MtrunA17_Chr1g0187921 99.153 236 2 0 1 236 1 236 1.38e-175 481
Msa0030270 MtrunA17_Chr1g0184791 69.038 239 50 7 12 236 17 245 4.85e-103 298
Msa0030270 MtrunA17_Chr7g0260641 64.113 248 66 5 6 236 9 250 4.88e-98 286
Msa0030270 MtrunA17_Chr1g0190711 62.705 244 71 6 8 236 15 253 1.04e-95 280
Msa0030270 MtrunA17_Chr6g0486121 51.562 256 85 6 6 236 13 254 1.91e-78 236
Msa0030270 MtrunA17_Chr8g0390281 48.364 275 93 8 7 236 23 293 5.24e-71 219
Msa0030270 MtrunA17_Chr5g0411501 48.252 286 90 7 8 236 51 335 1.22e-70 219
Msa0030270 MtrunA17_Chr8g0366881 44.755 286 101 5 6 236 65 348 7.24e-68 213
Msa0030270 MtrunA17_Chr2g0331411 43.894 303 95 8 8 236 25 326 1.35e-66 208
Msa0030270 MtrunA17_Chr5g0428141 62.893 159 57 2 78 236 191 347 2.75e-66 208
Msa0030270 MtrunA17_Chr1g0184801 46.429 224 79 6 8 224 12 201 6.63e-54 172
Msa0030270 MtrunA17_Chr1g0187961 45.662 219 70 6 6 224 9 178 4.45e-52 166
Msa0030270 MtrunA17_Chr7g0260621 45.662 219 91 6 8 225 11 202 3.08e-51 165
Msa0030270 MtrunA17_Chr1g0166011 42.986 221 83 6 8 224 1 182 1.93e-49 160
Msa0030270 MtrunA17_Chr4g0004181 41.304 230 75 7 1 228 1 172 1.38e-38 132
Msa0030270 MtrunA17_Chr1g0183251 42.623 183 72 3 82 231 81 263 1.09e-37 132
Msa0030270 MtrunA17_Chr2g0332141 53.846 117 43 4 116 228 4 113 7.31e-35 120
Msa0030270 MtrunA17_Chr8g0340731 38.150 173 91 3 64 220 88 260 1.68e-29 111
Msa0030270 MtrunA17_Chr2g0330951 39.412 170 72 5 77 220 90 254 2.91e-28 108
Msa0030270 MtrunA17_Chr4g0069561 32.867 143 67 4 81 221 69 184 2.27e-19 82.4
Msa0030270 MtrunA17_Chr3g0137601 26.950 141 83 3 83 221 88 210 3.67e-17 77.4
Msa0030270 MtrunA17_Chr1g0193861 41.509 106 48 3 113 217 78 170 4.18e-17 76.3
Msa0030270 MtrunA17_Chr4g0064131 26.667 135 78 1 84 218 93 206 4.28e-15 71.6
Msa0030270 MtrunA17_Chr7g0270851 39.604 101 47 4 118 217 68 155 1.91e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0030270 AT4G14550.1 75.424 236 43 4 8 236 1 228 4.20e-121 344
Msa0030270 AT3G23050.1 70.683 249 50 6 4 236 2 243 8.96e-118 336
Msa0030270 AT4G14550.2 74.554 224 42 4 8 224 1 216 7.60e-113 323
Msa0030270 AT1G04250.1 65.272 239 57 5 8 236 7 229 2.03e-103 299
Msa0030270 AT3G04730.1 64.050 242 67 4 8 236 2 236 6.99e-103 298
Msa0030270 AT4G14550.3 72.906 203 40 4 8 203 1 195 2.49e-98 285
Msa0030270 AT3G23050.2 68.056 216 47 5 4 204 2 210 5.44e-95 277
Msa0030270 AT4G29080.1 48.699 269 99 8 6 235 36 304 3.90e-71 220
Msa0030270 AT5G65670.2 44.964 278 102 6 8 236 61 336 1.57e-66 209
Msa0030270 AT5G65670.1 44.643 280 102 7 8 236 61 338 1.80e-64 204
Msa0030270 AT2G22670.3 53.271 214 79 5 41 235 107 318 1.28e-63 201
Msa0030270 AT2G22670.1 53.271 214 79 5 41 235 107 318 1.28e-63 201
Msa0030270 AT2G22670.4 53.271 214 79 5 41 235 124 335 1.85e-63 201
Msa0030270 AT4G14550.4 67.296 159 37 4 8 159 1 151 5.96e-63 195
Msa0030270 AT2G22670.5 52.336 214 79 6 41 235 107 316 2.32e-60 192
Msa0030270 AT2G22670.2 52.336 214 79 6 41 235 107 316 2.32e-60 192
Msa0030270 AT3G23050.3 60.694 173 46 5 4 161 2 167 1.25e-59 186
Msa0030270 AT1G04240.1 47.059 221 81 7 4 224 1 185 4.80e-54 172
Msa0030270 AT1G04240.2 46.429 224 84 7 1 224 98 285 2.62e-53 174
Msa0030270 AT5G43700.1 48.624 218 66 7 8 225 11 182 4.14e-53 170
Msa0030270 AT3G23030.1 44.240 217 66 5 8 224 9 170 4.91e-49 159
Msa0030270 AT3G23030.2 44.240 217 66 5 8 224 96 257 2.83e-48 160
Msa0030270 AT4G14560.1 43.056 216 67 5 6 221 5 164 6.12e-44 145
Msa0030270 AT1G15580.1 40.639 219 70 4 1 219 1 159 2.40e-43 144
Msa0030270 AT3G15540.1 40.517 232 94 7 6 231 4 197 2.66e-41 140
Msa0030270 AT2G33310.3 44.828 174 72 6 76 230 68 236 4.33e-39 135
Msa0030270 AT2G33310.1 44.828 174 72 6 76 230 68 236 4.33e-39 135
Msa0030270 AT2G33310.2 44.767 172 71 6 78 230 71 237 5.63e-38 133
Msa0030270 AT1G52830.1 38.356 219 97 6 6 224 4 184 2.17e-37 129
Msa0030270 AT4G28640.1 49.045 157 64 5 83 225 88 242 5.45e-36 127
Msa0030270 AT4G28640.3 49.032 155 63 5 83 223 88 240 9.23e-36 127
Msa0030270 AT1G04550.2 41.477 176 76 3 76 231 62 230 1.20e-35 127
Msa0030270 AT4G28640.2 49.032 155 63 5 83 223 88 240 1.82e-34 125
Msa0030270 AT3G16500.1 37.662 231 109 8 11 220 36 252 2.84e-33 121
Msa0030270 AT3G16500.2 37.662 231 109 8 11 220 36 252 4.02e-33 121
Msa0030270 AT5G25890.1 39.157 166 77 3 62 227 29 170 2.83e-30 111
Msa0030270 AT1G04100.1 42.515 167 67 4 85 224 95 259 7.18e-30 112
Msa0030270 AT1G51950.1 41.250 160 73 4 80 220 93 250 6.52e-29 110
Msa0030270 AT3G17600.1 32.877 146 68 4 75 217 36 154 2.92e-21 87.0
Msa0030270 AT2G46990.1 39.623 106 51 2 114 219 80 172 7.15e-19 81.3
Msa0030270 AT3G62100.1 38.889 108 53 2 110 217 74 168 1.62e-18 80.1
Msa0030270 AT2G46990.2 40.659 91 41 2 114 204 80 157 3.70e-14 68.2
Msa0030270 AT4G32280.1 24.413 213 104 6 50 232 64 249 1.42e-13 68.6
Msa0030270 AT1G80390.1 34.000 150 61 3 9 158 15 126 1.53e-13 65.9
Msa0030270 AT3G62100.2 37.895 95 46 2 110 204 74 155 1.99e-13 66.2
Msa0030270 AT4G32280.2 29.204 113 56 2 120 232 119 207 1.49e-12 65.1

Find 105 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAAGGAGCTGAACATTT+TGG 0.275554 1_1:+56366871 None:intergenic
GGTGGTGGTGGCGGCGGCGG+TGG 0.280712 1_1:-56367999 Msa0030270:CDS
ATATATATTATAGGTAACTA+TGG 0.289007 1_1:-56366451 Msa0030270:intron
AATCTTCTTCTTCTAAGCTT+TGG 0.317444 1_1:-56368111 Msa0030270:five_prime_UTR
ATTGAAGAACTTTGTGTTTG+TGG 0.337731 1_1:+56368323 None:intergenic
CTTCTCATTCAGATCTTCCT+TGG 0.341646 1_1:+56367893 None:intergenic
GGTGGTGGCGGCGGCGGTGG+AGG 0.343665 1_1:-56367996 Msa0030270:CDS
TACTTAGCTGGTGGCTTAGC+AGG 0.347183 1_1:+56367837 None:intergenic
GGTGGCGGCGGCGGTGGAGG+TGG 0.350359 1_1:-56367993 Msa0030270:CDS
TTGCATTTCTCCATTGCTCT+TGG 0.358710 1_1:+56365446 None:intergenic
TATTATAATGCACCTTGGTT+TGG 0.368189 1_1:+56368244 None:intergenic
GACAAAGCTCAGTCTCCTTC+AGG 0.370771 1_1:+56368033 None:intergenic
ATGAAAGGATCAGAAGCTAT+TGG 0.371063 1_1:-56366089 Msa0030270:CDS
TGTTGATTGGAGTGTTAGTT+TGG 0.376698 1_1:-56365324 Msa0030270:three_prime_UTR
CCTGGTGGTGGTGGCGGCGG+CGG 0.379613 1_1:-56368002 Msa0030270:CDS
GGCATCAGATAAATCTTTGT+AGG 0.380510 1_1:+56366898 None:intergenic
GTGGCTTAGCAGGGTCCTTA+AGG 0.389548 1_1:+56367847 None:intergenic
TACTGCTGCTGCATTTGTTA+AGG 0.392669 1_1:-56366975 Msa0030270:CDS
CATGAATGAGAGCAAGTTGA+TGG 0.395903 1_1:-56366407 Msa0030270:CDS
TCAATTAACTTATATTATTG+AGG 0.412560 1_1:-56368305 Msa0030270:five_prime_UTR
CTGCCTGGTGGTGGTGGCGG+CGG 0.419116 1_1:-56368005 Msa0030270:CDS
TGAAAGGATCAGAAGCTATT+GGG 0.427068 1_1:-56366088 Msa0030270:CDS
ATTGGGAAAACTTACTTAGC+TGG 0.432635 1_1:+56367825 None:intergenic
CAATTAACTTATATTATTGA+GGG 0.435956 1_1:-56368304 Msa0030270:five_prime_UTR
TCATGAAGTCTATCATTCCT+TGG 0.436607 1_1:+56366425 None:intergenic
GAGCTTTGTCTTGGTCTGCC+TGG 0.443783 1_1:-56368020 Msa0030270:CDS
ATGAAGCACTACCTGTTGAT+TGG 0.446997 1_1:-56365337 Msa0030270:three_prime_UTR
TTTGTTAAGGTTTCCATGGA+TGG 0.449811 1_1:-56366962 Msa0030270:CDS
AAGGAGACTGAGCTTTGTCT+TGG 0.456647 1_1:-56368029 Msa0030270:CDS
GGATCAGAAGCTATTGGGCT+TGG 0.459856 1_1:-56366083 Msa0030270:intron
CTTGGTCTGCCTGGTGGTGG+TGG 0.460328 1_1:-56368011 Msa0030270:CDS
CATGAAGTCTATCATTCCTT+GGG 0.461454 1_1:+56366426 None:intergenic
TATTTCAGGGCTCAAGTAGT+TGG 0.461914 1_1:-56367085 Msa0030270:intron
TGTCTTGGTCTGCCTGGTGG+TGG 0.462328 1_1:-56368014 Msa0030270:CDS
TATATATTATAGGTAACTAT+GGG 0.462994 1_1:-56366450 Msa0030270:intron
AAAGGAGAAGACCCTCCTTA+AGG 0.463373 1_1:-56367862 Msa0030270:CDS
TTGGTACGGGACTTCGATAT+AGG 0.464620 1_1:+56365383 None:intergenic
GTCGCCATCCTTATCTTCAT+AGG 0.465608 1_1:+56366354 None:intergenic
GAAGAACATGATGGCACAAA+AGG 0.465863 1_1:-56367038 Msa0030270:CDS
CTTATAGGTCCACTTTGGTA+CGG 0.467220 1_1:+56365369 None:intergenic
AAACTAACACTCCAATCAAC+AGG 0.468673 1_1:+56365326 None:intergenic
CCTCTCTTCGCAGAAGCCCT+TGG 0.474958 1_1:+56367954 None:intergenic
ATTTAGACCAAAATCAGCTT+CGG 0.485181 1_1:+56365420 None:intergenic
GCTTAGCAGGGTCCTTAAGG+AGG 0.489152 1_1:+56367850 None:intergenic
TTATAGGTCCACTTTGGTAC+GGG 0.492481 1_1:+56365370 None:intergenic
GGCAAACTACAATAGTATAT+AGG 0.494578 1_1:+56365210 None:intergenic
GGCCACCAGTGAGGTCATAC+AGG 0.498221 1_1:-56367060 Msa0030270:CDS
GGTCTGCCTGGTGGTGGTGG+CGG 0.503771 1_1:-56368008 Msa0030270:CDS
TGTGTTGATGTTTCTCCTCA+AGG 0.507891 1_1:+56368205 None:intergenic
CCATCCTTATCTTCATAGGT+TGG 0.508321 1_1:+56366358 None:intergenic
CGGTGGAGGTGGTTCTGAGG+TGG 0.509616 1_1:-56367982 Msa0030270:CDS
GAATGAGAAGAGTGCTTCAA+AGG 0.512968 1_1:-56367880 Msa0030270:CDS
TGCATTTGTTAAGGTTTCCA+TGG 0.525650 1_1:-56366966 Msa0030270:CDS
CCGCCGCCGCCACCACCACC+AGG 0.530029 1_1:+56368002 None:intergenic
TGTGCCAACCTATGAAGATA+AGG 0.537235 1_1:-56366362 Msa0030270:CDS
TCAGGGCTCAAGTAGTTGGT+TGG 0.538535 1_1:-56367081 Msa0030270:intron
ACTTAGCTGGTGGCTTAGCA+GGG 0.540758 1_1:+56367838 None:intergenic
TCTTGTATTATAATGCACCT+TGG 0.543903 1_1:+56368239 None:intergenic
TTCTTCCTGTATGACCTCAC+TGG 0.545123 1_1:+56367055 None:intergenic
AAGGTAAGGTGCTCCATCCA+TGG 0.548189 1_1:+56366949 None:intergenic
TGCGAATTTCACATCATGCT+AGG 0.551834 1_1:+56368351 None:intergenic
AAGTCAACCTTACGAAGGTA+AGG 0.557851 1_1:+56366935 None:intergenic
TGCTCGTGGGAGATGTCCCA+TGG 0.560169 1_1:-56366327 Msa0030270:CDS
GTTCTGAGGTGGAAACTCCA+AGG 0.563503 1_1:-56367971 Msa0030270:CDS
TGGAGCACCTTACCTTCGTA+AGG 0.564503 1_1:-56366942 Msa0030270:CDS
GTGTTGATGTTTCTCCTCAA+GGG 0.566405 1_1:+56368206 None:intergenic
CCAAGGGCTTCTGCGAAGAG+AGG 0.567370 1_1:-56367954 Msa0030270:CDS
CATTTCAGCACCAAGAGCAA+TGG 0.570918 1_1:-56365456 Msa0030270:intron
ATGTTCAGCTCCTTCACCAC+TGG 0.578252 1_1:-56366866 Msa0030270:intron
ATTAACTTATATTATTGAGG+GGG 0.578604 1_1:-56368302 Msa0030270:five_prime_UTR
TAATTAATTACCAGTGGTGA+AGG 0.579528 1_1:+56366856 None:intergenic
AATATGGCCACTATGGGGCA+TGG 0.587296 1_1:-56368062 Msa0030270:CDS
CTTAGCAGGGTCCTTAAGGA+GGG 0.587502 1_1:+56367851 None:intergenic
GTTCAATCCATGCCCCATAG+TGG 0.588585 1_1:+56368055 None:intergenic
ATCGAAGTCCCGTACCAAAG+TGG 0.594032 1_1:-56365378 Msa0030270:three_prime_UTR
AAGCGTCTGCGCATAATGAA+AGG 0.596352 1_1:-56366104 Msa0030270:CDS
GGATGGCGACTGGATGCTCG+TGG 0.596688 1_1:-56366341 Msa0030270:CDS
ATGAAGATAAGGATGGCGAC+TGG 0.598557 1_1:-56366351 Msa0030270:CDS
GCTATAGGAACTTACTCCCA+TGG 0.598945 1_1:+56366310 None:intergenic
AATTAACTTATATTATTGAG+GGG 0.603113 1_1:-56368303 Msa0030270:five_prime_UTR
TTCTGAGGTGGAAACTCCAA+GGG 0.603496 1_1:-56367970 Msa0030270:CDS
GGGAAAACTTACTTAGCTGG+TGG 0.604797 1_1:+56367828 None:intergenic
CGGCGGTGGAGGTGGTTCTG+AGG 0.606000 1_1:-56367985 Msa0030270:CDS
TTCCTGTATGACCTCACTGG+TGG 0.606534 1_1:+56367058 None:intergenic
ATAGGTAACTATGGGGCCCA+AGG 0.608548 1_1:-56366442 Msa0030270:intron
GTACGGGACTTCGATATAGG+AGG 0.608615 1_1:+56365386 None:intergenic
GAAGCTTAATCTTCAAACCA+AGG 0.609730 1_1:-56367910 Msa0030270:CDS
CTTTGTCTTGGTCTGCCTGG+TGG 0.610253 1_1:-56368017 Msa0030270:CDS
AAGGTGCATTATAATACAAG+AGG 0.617418 1_1:-56368237 Msa0030270:five_prime_UTR
CTATAGGAACTTACTCCCAT+GGG 0.622755 1_1:+56366311 None:intergenic
GGGGCATGGATTGAACCTGA+AGG 0.627746 1_1:-56368048 Msa0030270:CDS
CCAACCTATGAAGATAAGGA+TGG 0.646142 1_1:-56366358 Msa0030270:CDS
GCTCGTGGGAGATGTCCCAT+GGG 0.650152 1_1:-56366326 Msa0030270:CDS
AAACTACAATAGTATATAGG+TGG 0.655587 1_1:+56365213 None:intergenic
GTCATACAGGAAGAACATGA+TGG 0.666998 1_1:-56367047 Msa0030270:CDS
GATGGCGACTGGATGCTCGT+GGG 0.667120 1_1:-56366340 Msa0030270:CDS
AAAAGAATATGGCCACTATG+GGG 0.689548 1_1:-56368067 Msa0030270:exon
TAGTTGGTTGGCCACCAGTG+AGG 0.691007 1_1:-56367069 Msa0030270:CDS
ACACAACAAACATGACAGCA+TGG 0.695455 1_1:+56365156 None:intergenic
ATTTCTATATAGCCAAACCA+AGG 0.701377 1_1:-56368256 Msa0030270:five_prime_UTR
ATAATAGTAATAATTAAACA+AGG 0.703492 1_1:+56365189 None:intergenic
AAGAGGACAGTACACCCTTG+AGG 0.704580 1_1:-56368220 Msa0030270:five_prime_UTR
TTGTCAAGTCAACCTTACGA+AGG 0.716471 1_1:+56366930 None:intergenic
ACAAAAGGTTAGCAATACTG+AGG 0.716616 1_1:-56367023 Msa0030270:CDS
ATATATTATAGGTAACTATG+GGG 0.717921 1_1:-56366449 Msa0030270:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTAATATTATTATA+AGG - chr1_1:56366851-56366870 Msa0030270:intron 0.0%
!! TATTAATTTATATATATTAT+AGG - chr1_1:56366994-56367013 Msa0030270:CDS 0.0%
!!! TTTTTTTTTTATAACTTTCT+TGG - chr1_1:56365680-56365699 Msa0030270:intron 10.0%
!!! ATTATTAATTTGAAGAAAAA+TGG + chr1_1:56366209-56366228 None:intergenic 10.0%
!! TTAAAAAATTGCATAAAATT+TGG + chr1_1:56366287-56366306 None:intergenic 10.0%
!! ATAATAGTAATAATTAAACA+AGG + chr1_1:56368268-56368287 None:intergenic 10.0%
!! TCAATTAACTTATATTATTG+AGG - chr1_1:56365149-56365168 Msa0030270:three_prime_UTR 15.0%
!! CAATTAACTTATATTATTGA+GGG - chr1_1:56365150-56365169 Msa0030270:three_prime_UTR 15.0%
!! AATTAACTTATATTATTGAG+GGG - chr1_1:56365151-56365170 Msa0030270:three_prime_UTR 15.0%
!! AATATGAAAAAACGATATAA+GGG + chr1_1:56365979-56365998 None:intergenic 15.0%
!! AAAGAATAATTGAGATTTAT+AGG - chr1_1:56366319-56366338 Msa0030270:intron 15.0%
!! AAGAATAATTGAGATTTATA+GGG - chr1_1:56366320-56366339 Msa0030270:intron 15.0%
!! TAGATTAATTATTATCATCT+TGG - chr1_1:56366924-56366943 Msa0030270:CDS 15.0%
!! AGATTAATTATTATCATCTT+GGG - chr1_1:56366925-56366944 Msa0030270:CDS 15.0%
!! ATATATATTATAGGTAACTA+TGG - chr1_1:56367003-56367022 Msa0030270:CDS 15.0%
!! TATATATTATAGGTAACTAT+GGG - chr1_1:56367004-56367023 Msa0030270:CDS 15.0%
!!! TGGTTATTTAAATTTTTTGA+AGG - chr1_1:56367309-56367328 Msa0030270:intron 15.0%
!!! TTCAATGATTTATTTTTTGT+TGG + chr1_1:56367606-56367625 None:intergenic 15.0%
!! ATGATAATTTAGATATTTGA+AGG + chr1_1:56367748-56367767 None:intergenic 15.0%
!! AAATCATATTTACATTATGT+TGG + chr1_1:56367798-56367817 None:intergenic 15.0%
!! TGTAAATATGATTTAATAGT+TGG - chr1_1:56367804-56367823 Msa0030270:intron 15.0%
!! ATTAACTTATATTATTGAGG+GGG - chr1_1:56365152-56365171 Msa0030270:three_prime_UTR 20.0%
!!! TTGTTTAGTAAATGTTGTTT+TGG + chr1_1:56365737-56365756 None:intergenic 20.0%
!! AAAAAATAGAGAGAGAAAAA+AGG + chr1_1:56365856-56365875 None:intergenic 20.0%
!!! TTTCTTTTCTATCTTCTTAT+TGG + chr1_1:56365885-56365904 None:intergenic 20.0%
!! AATAAGAAGATAGAAAAGAA+AGG - chr1_1:56365884-56365903 Msa0030270:intron 20.0%
!! TATACTATTTGTATATGAGT+TGG - chr1_1:56365948-56365967 Msa0030270:intron 20.0%
!! ATATGAAAAAACGATATAAG+GGG + chr1_1:56365978-56365997 None:intergenic 20.0%
!! CAATATGAAAAAACGATATA+AGG + chr1_1:56365980-56365999 None:intergenic 20.0%
!! AATTTACTAAACAAGCATTA+TGG - chr1_1:56366021-56366040 Msa0030270:intron 20.0%
!!! TTTTTTTCTATCTTCCATTT+GGG + chr1_1:56366056-56366075 None:intergenic 20.0%
!!! TTTTTTTTCTATCTTCCATT+TGG + chr1_1:56366057-56366076 None:intergenic 20.0%
!! AATATTACAAATACAAGCAT+AGG + chr1_1:56366642-56366661 None:intergenic 20.0%
!! ATAAACTATATCAAATAGCT+AGG + chr1_1:56366778-56366797 None:intergenic 20.0%
!! ATATATTATAGGTAACTATG+GGG - chr1_1:56367005-56367024 Msa0030270:CDS 20.0%
!! AGTACATATATATGTTTGTT+GGG + chr1_1:56367242-56367261 None:intergenic 20.0%
!! AAGTACATATATATGTTTGT+TGG + chr1_1:56367243-56367262 None:intergenic 20.0%
!! AACAAACATATATATGTACT+TGG - chr1_1:56367242-56367261 Msa0030270:intron 20.0%
!! AATAACCAATGTTAATTTCA+TGG + chr1_1:56367297-56367316 None:intergenic 20.0%
!!! AATATTTTGTACTTAACTCA+TGG - chr1_1:56367425-56367444 Msa0030270:intron 20.0%
!!! TTTATTTTTTGTTGGTCTTA+AGG + chr1_1:56367598-56367617 None:intergenic 20.0%
!! ATTGAATTGAAAATCTTAGA+TGG - chr1_1:56367620-56367639 Msa0030270:intron 20.0%
!!! CTTCATATATCTTTTCTTAT+AGG + chr1_1:56368103-56368122 None:intergenic 20.0%
!!! TTTTCAGTTTTTTCATAGAG+AGG - chr1_1:56365274-56365293 Msa0030270:three_prime_UTR 25.0%
!!! TTTCAGTTTTTTCATAGAGA+GGG - chr1_1:56365275-56365294 Msa0030270:three_prime_UTR 25.0%
! TCAAAATTACAAGCATTTGT+GGG + chr1_1:56366162-56366181 None:intergenic 25.0%
! ATCAAAATTACAAGCATTTG+TGG + chr1_1:56366163-56366182 None:intergenic 25.0%
!!! TTATGCAATTTTTTAAGCCT+TGG - chr1_1:56366291-56366310 Msa0030270:intron 25.0%
! TAGTACTAATTAATTACCAG+TGG + chr1_1:56366607-56366626 None:intergenic 25.0%
!!! ACTTTTTTTTGTTGTGATGA+TGG + chr1_1:56366830-56366849 None:intergenic 25.0%
!!! TATAAGGTTTTAGATCTTGA+AGG - chr1_1:56366867-56366886 Msa0030270:CDS 25.0%
!! GTACATATATATGTTTGTTG+GGG + chr1_1:56367241-56367260 None:intergenic 25.0%
!!! ATTTTTTGAAGGATGTTTGT+TGG - chr1_1:56367320-56367339 Msa0030270:intron 25.0%
! GGCTATTTATTCTAACTTAT+TGG - chr1_1:56367958-56367977 Msa0030270:CDS 25.0%
! AAACTACAATAGTATATAGG+TGG + chr1_1:56368244-56368263 None:intergenic 25.0%
ATTGAAGAACTTTGTGTTTG+TGG + chr1_1:56365134-56365153 None:intergenic 30.0%
ATTTCTATATAGCCAAACCA+AGG - chr1_1:56365198-56365217 Msa0030270:three_prime_UTR 30.0%
TATTATAATGCACCTTGGTT+TGG + chr1_1:56365213-56365232 None:intergenic 30.0%
TCTTGTATTATAATGCACCT+TGG + chr1_1:56365218-56365237 None:intergenic 30.0%
AAGGTGCATTATAATACAAG+AGG - chr1_1:56365217-56365236 Msa0030270:three_prime_UTR 30.0%
AATCTTCTTCTTCTAAGCTT+TGG - chr1_1:56365343-56365362 Msa0030270:three_prime_UTR 30.0%
AAAAAGAATATGGCCACTAT+GGG - chr1_1:56365386-56365405 Msa0030270:three_prime_UTR 30.0%
AGCATGCAAGTACTTTAATT+GGG + chr1_1:56365649-56365668 None:intergenic 30.0%
AAGCATGCAAGTACTTTAAT+TGG + chr1_1:56365650-56365669 None:intergenic 30.0%
ATATTTGTACAACTGTTCTG+TGG - chr1_1:56365781-56365800 Msa0030270:intron 30.0%
! CAATTATTCTTTTCTCTCCA+AGG + chr1_1:56366311-56366330 None:intergenic 30.0%
TAATTAATTACCAGTGGTGA+AGG + chr1_1:56366601-56366620 None:intergenic 30.0%
CTATATCAAATAGCTAGGTA+TGG + chr1_1:56366773-56366792 None:intergenic 30.0%
TGATTGTAGTATATAGTAGC+TGG + chr1_1:56367187-56367206 None:intergenic 30.0%
TTCATGGATGTGTAAATATG+TGG + chr1_1:56367281-56367300 None:intergenic 30.0%
CACATCCATGAAATTAACAT+TGG - chr1_1:56367289-56367308 Msa0030270:intron 30.0%
! TGGTCTTAAGGATTAAGTTT+AGG + chr1_1:56367586-56367605 None:intergenic 30.0%
ATTTAGACCAAAATCAGCTT+CGG + chr1_1:56368037-56368056 None:intergenic 30.0%
GGCAAACTACAATAGTATAT+AGG + chr1_1:56368247-56368266 None:intergenic 30.0%
GAAGCTAGCCAAAAAGAATA+TGG - chr1_1:56365376-56365395 Msa0030270:three_prime_UTR 35.0%
! CATAGTGGCCATATTCTTTT+TGG + chr1_1:56365387-56365406 None:intergenic 35.0%
CAAAAAGAATATGGCCACTA+TGG - chr1_1:56365385-56365404 Msa0030270:three_prime_UTR 35.0%
AAAAGAATATGGCCACTATG+GGG - chr1_1:56365387-56365406 Msa0030270:three_prime_UTR 35.0%
GAAGCTTAATCTTCAAACCA+AGG - chr1_1:56365544-56365563 Msa0030270:intron 35.0%
ATTGGGAAAACTTACTTAGC+TGG + chr1_1:56365632-56365651 None:intergenic 35.0%
ATAGAGAGAGAAAAAAGGAG+AGG + chr1_1:56365851-56365870 None:intergenic 35.0%
TATGGATTCTCAAACCCAAA+TGG - chr1_1:56366039-56366058 Msa0030270:intron 35.0%
ACAAAAGGTTAGCAATACTG+AGG - chr1_1:56366431-56366450 Msa0030270:CDS 35.0%
! TGCATTTGTTAAGGTTTCCA+TGG - chr1_1:56366488-56366507 Msa0030270:intron 35.0%
TTTGTTAAGGTTTCCATGGA+TGG - chr1_1:56366492-56366511 Msa0030270:intron 35.0%
GGCATCAGATAAATCTTTGT+AGG + chr1_1:56366559-56366578 None:intergenic 35.0%
TAATCTTTCAGCATTCTGTC+TGG + chr1_1:56366697-56366716 None:intergenic 35.0%
AATAGCTAGGTATGGACTTT+AGG + chr1_1:56366765-56366784 None:intergenic 35.0%
TTTAGATCTTGAAGGATGCA+TGG - chr1_1:56366875-56366894 Msa0030270:CDS 35.0%
CATGAAGTCTATCATTCCTT+GGG + chr1_1:56367031-56367050 None:intergenic 35.0%
TCATGAAGTCTATCATTCCT+TGG + chr1_1:56367032-56367051 None:intergenic 35.0%
TAGTATATAGTAGCTGGAGA+TGG + chr1_1:56367181-56367200 None:intergenic 35.0%
TGGATGTGTAAATATGTGGT+TGG + chr1_1:56367277-56367296 None:intergenic 35.0%
ATGAAAGGATCAGAAGCTAT+TGG - chr1_1:56367365-56367384 Msa0030270:intron 35.0%
TGAAAGGATCAGAAGCTATT+GGG - chr1_1:56367366-56367385 Msa0030270:intron 35.0%
! TAACTCATGGAAGTATGCTT+TGG - chr1_1:56367438-56367457 Msa0030270:intron 35.0%
TGCATCAAGTATAATGCAAG+TGG + chr1_1:56367534-56367553 None:intergenic 35.0%
! CTTTTCTTATAGGTCCACTT+TGG + chr1_1:56368093-56368112 None:intergenic 35.0%
AAACTAACACTCCAATCAAC+AGG + chr1_1:56368131-56368150 None:intergenic 35.0%
! TGTTGATTGGAGTGTTAGTT+TGG - chr1_1:56368130-56368149 Msa0030270:five_prime_UTR 35.0%
! GTGTTGATGTTTCTCCTCAA+GGG + chr1_1:56365251-56365270 None:intergenic 40.0%
! TGTGTTGATGTTTCTCCTCA+AGG + chr1_1:56365252-56365271 None:intergenic 40.0%
CTTCTCATTCAGATCTTCCT+TGG + chr1_1:56365564-56365583 None:intergenic 40.0%
!! GAATGAGAAGAGTGCTTCAA+AGG - chr1_1:56365574-56365593 Msa0030270:intron 40.0%
TATTTCAGGGCTCAAGTAGT+TGG - chr1_1:56366369-56366388 Msa0030270:CDS 40.0%
GTCATACAGGAAGAACATGA+TGG - chr1_1:56366407-56366426 Msa0030270:CDS 40.0%
GAAGAACATGATGGCACAAA+AGG - chr1_1:56366416-56366435 Msa0030270:CDS 40.0%
! TACTGCTGCTGCATTTGTTA+AGG - chr1_1:56366479-56366498 Msa0030270:intron 40.0%
AAGTCAACCTTACGAAGGTA+AGG + chr1_1:56366522-56366541 None:intergenic 40.0%
TTGTCAAGTCAACCTTACGA+AGG + chr1_1:56366527-56366546 None:intergenic 40.0%
! CATTCTGTCTGGTTTAGATG+GGG + chr1_1:56366686-56366705 None:intergenic 40.0%
! GCATTCTGTCTGGTTTAGAT+GGG + chr1_1:56366687-56366706 None:intergenic 40.0%
! AGCATTCTGTCTGGTTTAGA+TGG + chr1_1:56366688-56366707 None:intergenic 40.0%
! CATGAATGAGAGCAAGTTGA+TGG - chr1_1:56367047-56367066 Msa0030270:CDS 40.0%
TGTGCCAACCTATGAAGATA+AGG - chr1_1:56367092-56367111 Msa0030270:intron 40.0%
CCATCCTTATCTTCATAGGT+TGG + chr1_1:56367099-56367118 None:intergenic 40.0%
CCAACCTATGAAGATAAGGA+TGG - chr1_1:56367096-56367115 Msa0030270:intron 40.0%
CTATAGGAACTTACTCCCAT+GGG + chr1_1:56367146-56367165 None:intergenic 40.0%
TATATAGTAGCTGGAGATGG+AGG + chr1_1:56367178-56367197 None:intergenic 40.0%
ACAAAATGACACGCACACAA+GGG + chr1_1:56367938-56367957 None:intergenic 40.0%
TTGCATTTCTCCATTGCTCT+TGG + chr1_1:56368011-56368030 None:intergenic 40.0%
!! GCAAAAACCGAAGCTGATTT+TGG - chr1_1:56368027-56368046 Msa0030270:CDS 40.0%
TTATAGGTCCACTTTGGTAC+GGG + chr1_1:56368087-56368106 None:intergenic 40.0%
CTTATAGGTCCACTTTGGTA+CGG + chr1_1:56368088-56368107 None:intergenic 40.0%
! ATGAAGCACTACCTGTTGAT+TGG - chr1_1:56368117-56368136 Msa0030270:five_prime_UTR 40.0%
ACACAACAAACATGACAGCA+TGG + chr1_1:56368301-56368320 None:intergenic 40.0%
! AAGGAGACTGAGCTTTGTCT+TGG - chr1_1:56365425-56365444 Msa0030270:exon 45.0%
!! TTCTGAGGTGGAAACTCCAA+GGG - chr1_1:56365484-56365503 Msa0030270:intron 45.0%
AAAGGAGAAGACCCTCCTTA+AGG - chr1_1:56365592-56365611 Msa0030270:intron 45.0%
GGGAAAACTTACTTAGCTGG+TGG + chr1_1:56365629-56365648 None:intergenic 45.0%
TTCTTCCTGTATGACCTCAC+TGG + chr1_1:56366402-56366421 None:intergenic 45.0%
!!! GGAGCTGAACATTTTGGCTA+AGG + chr1_1:56366580-56366599 None:intergenic 45.0%
! GGTGAAGGAGCTGAACATTT+TGG + chr1_1:56366586-56366605 None:intergenic 45.0%
GTCGCCATCCTTATCTTCAT+AGG + chr1_1:56367103-56367122 None:intergenic 45.0%
ATGAAGATAAGGATGGCGAC+TGG - chr1_1:56367103-56367122 Msa0030270:intron 45.0%
GCTATAGGAACTTACTCCCA+TGG + chr1_1:56367147-56367166 None:intergenic 45.0%
ATGGAGGTTGGATGAGCTAT+AGG + chr1_1:56367162-56367181 None:intergenic 45.0%
AAGCGTCTGCGCATAATGAA+AGG - chr1_1:56367350-56367369 Msa0030270:intron 45.0%
AAAATGACACGCACACAAGG+GGG + chr1_1:56367936-56367955 None:intergenic 45.0%
CAAAATGACACGCACACAAG+GGG + chr1_1:56367937-56367956 None:intergenic 45.0%
CACAAAATGACACGCACACA+AGG + chr1_1:56367939-56367958 None:intergenic 45.0%
!!! CTTGTGTGCGTGTCATTTTG+TGG - chr1_1:56367937-56367956 Msa0030270:CDS 45.0%
CATTTCAGCACCAAGAGCAA+TGG - chr1_1:56367998-56368017 Msa0030270:CDS 45.0%
TTGGTACGGGACTTCGATAT+AGG + chr1_1:56368074-56368093 None:intergenic 45.0%
!! TATTATATTAATAATATTTC+AGG - chr1_1:56366355-56366374 Msa0030270:CDS 5.0%
!! ATTATATTAATAATATTTCA+GGG - chr1_1:56366356-56366375 Msa0030270:CDS 5.0%
!! ATTTGTAATATTTATATATA+TGG - chr1_1:56366650-56366669 Msa0030270:intron 5.0%
AAGAGGACAGTACACCCTTG+AGG - chr1_1:56365234-56365253 Msa0030270:three_prime_UTR 50.0%
AATATGGCCACTATGGGGCA+TGG - chr1_1:56365392-56365411 Msa0030270:three_prime_UTR 50.0%
GTTCAATCCATGCCCCATAG+TGG + chr1_1:56365402-56365421 None:intergenic 50.0%
GACAAAGCTCAGTCTCCTTC+AGG + chr1_1:56365424-56365443 None:intergenic 50.0%
!! GTTCTGAGGTGGAAACTCCA+AGG - chr1_1:56365483-56365502 Msa0030270:intron 50.0%
CTTAGCAGGGTCCTTAAGGA+GGG + chr1_1:56365606-56365625 None:intergenic 50.0%
ACTTAGCTGGTGGCTTAGCA+GGG + chr1_1:56365619-56365638 None:intergenic 50.0%
TACTTAGCTGGTGGCTTAGC+AGG + chr1_1:56365620-56365639 None:intergenic 50.0%
TCAGGGCTCAAGTAGTTGGT+TGG - chr1_1:56366373-56366392 Msa0030270:CDS 50.0%
TTCCTGTATGACCTCACTGG+TGG + chr1_1:56366399-56366418 None:intergenic 50.0%
! AAGGTAAGGTGCTCCATCCA+TGG + chr1_1:56366508-56366527 None:intergenic 50.0%
! TGGAGCACCTTACCTTCGTA+AGG - chr1_1:56366512-56366531 Msa0030270:intron 50.0%
ATGTTCAGCTCCTTCACCAC+TGG - chr1_1:56366588-56366607 Msa0030270:intron 50.0%
AGGATGCATGGCGATTGTTG+AGG - chr1_1:56366887-56366906 Msa0030270:CDS 50.0%
ATAGGTAACTATGGGGCCCA+AGG - chr1_1:56367012-56367031 Msa0030270:CDS 50.0%
TAGTAGCTGGAGATGGAGGT+TGG + chr1_1:56367174-56367193 None:intergenic 50.0%
GGATCAGAAGCTATTGGGCT+TGG - chr1_1:56367371-56367390 Msa0030270:intron 50.0%
AAATGACACGCACACAAGGG+GGG + chr1_1:56367935-56367954 None:intergenic 50.0%
GTACGGGACTTCGATATAGG+AGG + chr1_1:56368071-56368090 None:intergenic 50.0%
ATCGAAGTCCCGTACCAAAG+TGG - chr1_1:56368076-56368095 Msa0030270:exon 50.0%
! GGGGCATGGATTGAACCTGA+AGG - chr1_1:56365406-56365425 Msa0030270:three_prime_UTR 55.0%
! GAGCTTTGTCTTGGTCTGCC+TGG - chr1_1:56365434-56365453 Msa0030270:CDS 55.0%
CTTTGTCTTGGTCTGCCTGG+TGG - chr1_1:56365437-56365456 Msa0030270:CDS 55.0%
GCTTAGCAGGGTCCTTAAGG+AGG + chr1_1:56365607-56365626 None:intergenic 55.0%
GTGGCTTAGCAGGGTCCTTA+AGG + chr1_1:56365610-56365629 None:intergenic 55.0%
! TAGTTGGTTGGCCACCAGTG+AGG - chr1_1:56366385-56366404 Msa0030270:CDS 55.0%
TGTCTTGGTCTGCCTGGTGG+TGG - chr1_1:56365440-56365459 Msa0030270:CDS 60.0%
CCTCTCTTCGCAGAAGCCCT+TGG + chr1_1:56365503-56365522 None:intergenic 60.0%
CCAAGGGCTTCTGCGAAGAG+AGG - chr1_1:56365500-56365519 Msa0030270:intron 60.0%
GGCCACCAGTGAGGTCATAC+AGG - chr1_1:56366394-56366413 Msa0030270:CDS 60.0%
GATGGCGACTGGATGCTCGT+GGG - chr1_1:56367114-56367133 Msa0030270:intron 60.0%
TGCTCGTGGGAGATGTCCCA+TGG - chr1_1:56367127-56367146 Msa0030270:intron 60.0%
GCTCGTGGGAGATGTCCCAT+GGG - chr1_1:56367128-56367147 Msa0030270:intron 60.0%
GCACACAAGGGGGGACTCAA+GGG + chr1_1:56367926-56367945 None:intergenic 60.0%
!! CTTGGTCTGCCTGGTGGTGG+TGG - chr1_1:56365443-56365462 Msa0030270:CDS 65.0%
!! CGGTGGAGGTGGTTCTGAGG+TGG - chr1_1:56365472-56365491 Msa0030270:intron 65.0%
GGATGGCGACTGGATGCTCG+TGG - chr1_1:56367113-56367132 Msa0030270:intron 65.0%
CGCACACAAGGGGGGACTCA+AGG + chr1_1:56367927-56367946 None:intergenic 65.0%
!! GGTCTGCCTGGTGGTGGTGG+CGG - chr1_1:56365446-56365465 Msa0030270:CDS 70.0%
! CGGCGGTGGAGGTGGTTCTG+AGG - chr1_1:56365469-56365488 Msa0030270:intron 70.0%
!! CTGCCTGGTGGTGGTGGCGG+CGG - chr1_1:56365449-56365468 Msa0030270:CDS 75.0%
!!! CCTGGTGGTGGTGGCGGCGG+CGG - chr1_1:56365452-56365471 Msa0030270:intron 80.0%
! CCGCCGCCGCCACCACCACC+AGG + chr1_1:56365455-56365474 None:intergenic 85.0%
!!! GGTGGTGGTGGCGGCGGCGG+TGG - chr1_1:56365455-56365474 Msa0030270:intron 85.0%
!!! GGTGGTGGCGGCGGCGGTGG+AGG - chr1_1:56365458-56365477 Msa0030270:intron 85.0%
!! GGTGGCGGCGGCGGTGGAGG+TGG - chr1_1:56365461-56365480 Msa0030270:intron 85.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 56365106 56368370 56365106 ID=Msa0030270;Name=Msa0030270
chr1_1 mRNA 56365106 56368370 56365106 ID=Msa0030270-mRNA-1;Parent=Msa0030270;Name=Msa0030270-mRNA-1;_AED=0.04;_eAED=0.04;_QI=289|1|1|1|1|1|5|327|236
chr1_1 exon 56365106 56365470 56365106 ID=Msa0030270-mRNA-1:exon:16823;Parent=Msa0030270-mRNA-1
chr1_1 exon 56366084 56366145 56366084 ID=Msa0030270-mRNA-1:exon:16822;Parent=Msa0030270-mRNA-1
chr1_1 exon 56366325 56366460 56366325 ID=Msa0030270-mRNA-1:exon:16821;Parent=Msa0030270-mRNA-1
chr1_1 exon 56366867 56367099 56366867 ID=Msa0030270-mRNA-1:exon:16820;Parent=Msa0030270-mRNA-1
chr1_1 exon 56367840 56368370 56367840 ID=Msa0030270-mRNA-1:exon:16819;Parent=Msa0030270-mRNA-1
chr1_1 five_prime_UTR 56368082 56368370 56368082 ID=Msa0030270-mRNA-1:five_prime_utr;Parent=Msa0030270-mRNA-1
chr1_1 CDS 56367840 56368081 56367840 ID=Msa0030270-mRNA-1:cds;Parent=Msa0030270-mRNA-1
chr1_1 CDS 56366867 56367099 56366867 ID=Msa0030270-mRNA-1:cds;Parent=Msa0030270-mRNA-1
chr1_1 CDS 56366325 56366460 56366325 ID=Msa0030270-mRNA-1:cds;Parent=Msa0030270-mRNA-1
chr1_1 CDS 56366084 56366145 56366084 ID=Msa0030270-mRNA-1:cds;Parent=Msa0030270-mRNA-1
chr1_1 CDS 56365433 56365470 56365433 ID=Msa0030270-mRNA-1:cds;Parent=Msa0030270-mRNA-1
chr1_1 three_prime_UTR 56365106 56365432 56365106 ID=Msa0030270-mRNA-1:three_prime_utr;Parent=Msa0030270-mRNA-1
Gene Sequence

>Msa0030270

ATGGCCACTATGGGGCATGGATTGAACCTGAAGGAGACTGAGCTTTGTCTTGGTCTGCCTGGTGGTGGTGGCGGCGGCGGTGGAGGTGGTTCTGAGGTGGAAACTCCAAGGGCTTCTGCGAAGAGAGGTTTCTCTGAGACTGTTGATCTGAAGCTTAATCTTCAAACCAAGGAAGATCTGAATGAGAAGAGTGCTTCAAAGGAGAAGACCCTCCTTAAGGACCCTGCTAAGCCACCAGCTAAGGCTCAAGTAGTTGGTTGGCCACCAGTGAGGTCATACAGGAAGAACATGATGGCACAAAAGGTTAGCAATACTGAGGACACTGAGAAGACAACAAGTAACACTACTGCTGCTGCATTTGTTAAGGTTTCCATGGATGGAGCACCTTACCTTCGTAAGGTTGACTTGACAATGTACAAAACCTACAAAGATTTATCTGATGCCTTAGCCAAAATGTTCAGCTCCTTCACCACTGGTAACTATGGGGCCCAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTTAACAGCTCTGAGTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGAGATGTCCCATGGGAGATGTTTGTTGGATCATGCAAGCGTCTGCGCATAATGAAAGGATCAGAAGCTATTGGGCTTGCACCAAGAGCAATGGAGAAATGCAAAAACCGAAGCTGA
Protein sequence

>Msa0030270

MATMGHGLNLKETELCLGLPGGGGGGGGGGSEVETPRASAKRGFSETVDLKLNLQTKEDLNEKSASKEKTLLKDPAKPPAKAQVVGWPPVRSYRKNMMAQKVSNTEDTEKTTSNTTAAAFVKVSMDGAPYLRKVDLTMYKTYKDLSDALAKMFSSFTTGNYGAQGMIDFMNESKLMDLLNSSEYVPTYEDKDGDWMLVGDVPWEMFVGSCKRLRIMKGSEAIGLAPRAMEKCKNRS*