AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077870 MtrunA17_Chr1g0187921 99.576 236 1 0 1 236 1 236 1.92e-176 484
Msa0077870 MtrunA17_Chr1g0184791 68.619 239 51 7 12 236 17 245 1.80e-102 297
Msa0077870 MtrunA17_Chr7g0260641 63.710 248 67 5 6 236 9 250 6.21e-98 286
Msa0077870 MtrunA17_Chr1g0190711 63.115 244 70 6 8 236 15 253 1.55e-96 282
Msa0077870 MtrunA17_Chr6g0486121 50.973 257 85 6 6 236 13 254 6.56e-79 238
Msa0077870 MtrunA17_Chr8g0390281 48.364 275 93 8 7 236 23 293 5.35e-71 219
Msa0077870 MtrunA17_Chr5g0411501 48.252 286 90 7 8 236 51 335 1.20e-70 219
Msa0077870 MtrunA17_Chr8g0366881 44.755 286 101 5 6 236 65 348 6.09e-68 213
Msa0077870 MtrunA17_Chr2g0331411 43.894 303 95 8 8 236 25 326 1.99e-66 208
Msa0077870 MtrunA17_Chr5g0428141 62.893 159 57 2 78 236 191 347 2.58e-66 208
Msa0077870 MtrunA17_Chr1g0184801 46.429 224 79 6 8 224 12 201 8.23e-54 172
Msa0077870 MtrunA17_Chr1g0187961 46.083 217 68 6 8 224 11 178 4.50e-52 166
Msa0077870 MtrunA17_Chr7g0260621 46.119 219 90 6 8 225 11 202 5.49e-52 167
Msa0077870 MtrunA17_Chr1g0166011 42.986 221 83 6 8 224 1 182 1.83e-49 160
Msa0077870 MtrunA17_Chr4g0004181 41.304 230 75 7 1 228 1 172 1.60e-38 132
Msa0077870 MtrunA17_Chr1g0183251 42.623 183 72 3 82 231 81 263 1.32e-37 132
Msa0077870 MtrunA17_Chr2g0332141 53.846 117 43 4 116 228 4 113 8.33e-35 120
Msa0077870 MtrunA17_Chr8g0340731 37.714 175 89 3 64 220 88 260 2.76e-29 110
Msa0077870 MtrunA17_Chr2g0330951 38.824 170 73 5 77 220 90 254 3.63e-28 107
Msa0077870 MtrunA17_Chr4g0069561 32.867 143 67 4 81 221 69 184 2.42e-19 82.4
Msa0077870 MtrunA17_Chr1g0193861 41.509 106 48 3 113 217 78 170 4.59e-17 76.3
Msa0077870 MtrunA17_Chr3g0137601 26.950 141 83 3 83 221 88 210 5.20e-17 77.0
Msa0077870 MtrunA17_Chr4g0064131 26.667 135 78 1 84 218 93 206 3.42e-15 71.6
Msa0077870 MtrunA17_Chr7g0270851 39.604 101 47 4 118 217 68 155 1.95e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077870 AT4G14550.1 75.000 236 44 4 8 236 1 228 1.01e-120 343
Msa0077870 AT3G23050.1 70.683 249 50 6 4 236 2 243 2.01e-117 335
Msa0077870 AT4G14550.2 74.107 224 43 4 8 224 1 216 1.69e-112 322
Msa0077870 AT1G04250.1 65.272 239 57 5 8 236 7 229 2.01e-103 299
Msa0077870 AT3G04730.1 64.050 242 67 4 8 236 2 236 1.21e-102 297
Msa0077870 AT4G14550.3 72.414 203 41 4 8 203 1 195 7.44e-98 284
Msa0077870 AT3G23050.2 67.593 216 48 5 4 204 2 210 9.30e-95 276
Msa0077870 AT4G29080.1 48.699 269 99 8 6 235 36 304 3.62e-71 220
Msa0077870 AT5G65670.2 44.964 278 102 6 8 236 61 336 1.55e-66 209
Msa0077870 AT5G65670.1 44.643 280 102 7 8 236 61 338 1.46e-64 204
Msa0077870 AT2G22670.3 52.804 214 80 5 41 235 107 318 3.63e-63 200
Msa0077870 AT2G22670.1 52.804 214 80 5 41 235 107 318 3.63e-63 200
Msa0077870 AT2G22670.4 52.804 214 80 5 41 235 124 335 5.52e-63 200
Msa0077870 AT4G14550.4 66.667 159 38 4 8 159 1 151 1.87e-62 193
Msa0077870 AT2G22670.5 51.869 214 80 6 41 235 107 316 6.55e-60 191
Msa0077870 AT2G22670.2 51.869 214 80 6 41 235 107 316 6.55e-60 191
Msa0077870 AT3G23050.3 60.694 173 46 5 4 161 2 167 1.89e-59 185
Msa0077870 AT1G04240.1 47.511 221 80 7 4 224 1 185 7.56e-55 174
Msa0077870 AT1G04240.2 46.875 224 83 7 1 224 98 285 5.14e-54 175
Msa0077870 AT5G43700.1 48.165 218 67 7 8 225 11 182 1.65e-52 168
Msa0077870 AT3G23030.1 45.161 217 64 6 8 224 9 170 3.63e-49 159
Msa0077870 AT3G23030.2 45.161 217 64 6 8 224 96 257 2.33e-48 160
Msa0077870 AT4G14560.1 43.056 216 67 5 6 221 5 164 7.20e-44 145
Msa0077870 AT1G15580.1 40.639 219 70 4 1 219 1 159 2.53e-43 144
Msa0077870 AT3G15540.1 40.086 232 95 7 6 231 4 197 7.49e-41 139
Msa0077870 AT2G33310.3 44.828 174 72 6 76 230 68 236 4.98e-39 135
Msa0077870 AT2G33310.1 44.828 174 72 6 76 230 68 236 4.98e-39 135
Msa0077870 AT2G33310.2 44.767 172 71 6 78 230 71 237 6.90e-38 132
Msa0077870 AT1G52830.1 38.813 219 96 6 6 224 4 184 9.09e-38 130
Msa0077870 AT4G28640.1 48.408 157 65 5 83 225 88 242 1.73e-35 126
Msa0077870 AT1G04550.2 41.477 176 76 3 76 231 62 230 2.42e-35 126
Msa0077870 AT4G28640.3 48.387 155 64 5 83 223 88 240 2.95e-35 126
Msa0077870 AT3G16500.1 38.095 231 108 8 11 220 36 252 4.88e-34 123
Msa0077870 AT4G28640.2 48.387 155 64 5 83 223 88 240 5.36e-34 124
Msa0077870 AT3G16500.2 38.095 231 108 8 11 220 36 252 7.50e-34 122
Msa0077870 AT5G25890.1 39.157 166 77 3 62 227 29 170 2.74e-30 111
Msa0077870 AT1G04100.1 40.719 167 70 4 85 224 95 259 7.73e-30 112
Msa0077870 AT1G51950.1 40.625 160 74 4 80 220 93 250 2.28e-28 108
Msa0077870 AT3G17600.1 32.877 146 68 4 75 217 36 154 2.24e-21 87.4
Msa0077870 AT2G46990.1 39.623 106 51 2 114 219 80 172 8.02e-19 81.3
Msa0077870 AT3G62100.1 38.889 108 53 2 110 217 74 168 1.66e-18 80.1
Msa0077870 AT2G46990.2 40.659 91 41 2 114 204 80 157 3.98e-14 68.2
Msa0077870 AT4G32280.1 23.944 213 105 6 50 232 64 249 1.06e-13 68.9
Msa0077870 AT1G80390.1 34.000 150 61 3 9 158 15 126 1.60e-13 65.9
Msa0077870 AT3G62100.2 37.895 95 46 2 110 204 74 155 2.09e-13 66.2
Msa0077870 AT4G32280.2 29.204 113 56 2 120 232 119 207 1.49e-12 65.1

Find 105 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAAGGAGCTGAACATTT+TGG 0.275554 1_2:+54894408 None:intergenic
GGTGGTGGTGGCGGCGGCGG+TGG 0.280712 1_2:-54895537 Msa0077870:CDS
ATATATATTATAGGTAACTA+TGG 0.289007 1_2:-54893989 Msa0077870:intron
AATCTTCTTCTTCTAAGCTT+TGG 0.317444 1_2:-54895649 Msa0077870:five_prime_UTR
GGTGGTGGCGGCGGCGGTGG+AGG 0.335196 1_2:-54895534 Msa0077870:CDS
ATTGAAGAACTTTGTGTTTG+TGG 0.340808 1_2:+54895860 None:intergenic
CTTCTCATTCAGATCTTCCT+TGG 0.341646 1_2:+54895431 None:intergenic
TACTTAGCTGGTGGCTTAGC+AGG 0.347183 1_2:+54895375 None:intergenic
GGTGGCGGCGGCGGTGGAGG+AGG 0.347274 1_2:-54895531 Msa0077870:CDS
TTGCATTTCTCCATTGCTCT+TGG 0.358710 1_2:+54892984 None:intergenic
TATTATAATGCACCTTGGTT+TGG 0.368189 1_2:+54895779 None:intergenic
GACAAAGCTCAGTCTCCTTC+AGG 0.370771 1_2:+54895571 None:intergenic
ATGAAAGGATCAGAAGCTAT+TGG 0.371063 1_2:-54893627 Msa0077870:CDS
TGTTGATTGGAGTGTTAGTT+TGG 0.376698 1_2:-54892862 Msa0077870:three_prime_UTR
CCTGGTGGTGGTGGCGGCGG+CGG 0.379613 1_2:-54895540 Msa0077870:CDS
GGCATCAGATAAATCTTTGT+AGG 0.380510 1_2:+54894435 None:intergenic
GTGGCTTAGCAGGGTCCTTA+AGG 0.389548 1_2:+54895385 None:intergenic
TACTGCTGCTGCATTTGTTA+AGG 0.392669 1_2:-54894512 Msa0077870:CDS
CATGAATGAGAGCAAGTTGA+TGG 0.395903 1_2:-54893945 Msa0077870:CDS
TCAATTAACTTATATTATTG+AGG 0.412560 1_2:-54895842 Msa0077870:five_prime_UTR
CTGCCTGGTGGTGGTGGCGG+CGG 0.419116 1_2:-54895543 Msa0077870:CDS
TGAAAGGATCAGAAGCTATT+GGG 0.427068 1_2:-54893626 Msa0077870:CDS
ATTGGGAAAACTTACTTAGC+TGG 0.432635 1_2:+54895363 None:intergenic
CAATTAACTTATATTATTGA+GGG 0.435956 1_2:-54895841 Msa0077870:five_prime_UTR
TCATGAAGTCTATCATTCCT+TGG 0.436607 1_2:+54893963 None:intergenic
GAGCTTTGTCTTGGTCTGCC+TGG 0.443783 1_2:-54895558 Msa0077870:CDS
ATGAAGCACTACCTGTTGAT+TGG 0.446997 1_2:-54892875 Msa0077870:three_prime_UTR
TTTGTTAAGGTTTCCATGGA+TGG 0.449811 1_2:-54894499 Msa0077870:CDS
AAGGAGACTGAGCTTTGTCT+TGG 0.456647 1_2:-54895567 Msa0077870:CDS
GGATCAGAAGCTATTGGGCT+TGG 0.459856 1_2:-54893621 Msa0077870:intron
CTTGGTCTGCCTGGTGGTGG+TGG 0.460328 1_2:-54895549 Msa0077870:CDS
CATGAAGTCTATCATTCCTT+GGG 0.461454 1_2:+54893964 None:intergenic
TATTTCAGGGCTCAAGTAGT+TGG 0.461914 1_2:-54894622 Msa0077870:intron
TGTCTTGGTCTGCCTGGTGG+TGG 0.462328 1_2:-54895552 Msa0077870:CDS
TATATATTATAGGTAACTAT+GGG 0.462994 1_2:-54893988 Msa0077870:intron
AAAGGAGAAGACCCTCCTTA+AGG 0.463373 1_2:-54895400 Msa0077870:CDS
TTGGTACGGGACTTCGATAT+AGG 0.464620 1_2:+54892921 None:intergenic
GTCGCCATCCTTATCTTCAT+AGG 0.465608 1_2:+54893892 None:intergenic
GAAGAACATGATGGCACAAA+AGG 0.465863 1_2:-54894575 Msa0077870:CDS
CTTATAGGTCCACTTTGGTA+CGG 0.467220 1_2:+54892907 None:intergenic
AAACTAACACTCCAATCAAC+AGG 0.468673 1_2:+54892864 None:intergenic
CCTCTCTTCGCAGAAGCCCT+TGG 0.474958 1_2:+54895492 None:intergenic
ATTTAGACCAAAATCAGCTT+CGG 0.485181 1_2:+54892958 None:intergenic
GCTTAGCAGGGTCCTTAAGG+AGG 0.489152 1_2:+54895388 None:intergenic
TTATAGGTCCACTTTGGTAC+GGG 0.492481 1_2:+54892908 None:intergenic
GGCAAACTACAATAGTATAT+AGG 0.494578 1_2:+54892748 None:intergenic
GGCCACCAGTGAGGTCATAC+AGG 0.498221 1_2:-54894597 Msa0077870:CDS
GGTCTGCCTGGTGGTGGTGG+CGG 0.503771 1_2:-54895546 Msa0077870:CDS
TGTGTTGATGTTTCTCCTCA+AGG 0.507891 1_2:+54895743 None:intergenic
CCATCCTTATCTTCATAGGT+TGG 0.508321 1_2:+54893896 None:intergenic
GAATGAGAAGAGTGCTTCAA+AGG 0.512968 1_2:-54895418 Msa0077870:CDS
TGCATTTGTTAAGGTTTCCA+TGG 0.525650 1_2:-54894503 Msa0077870:CDS
CCGCCGCCGCCACCACCACC+AGG 0.530029 1_2:+54895540 None:intergenic
TGTGCCAACCTATGAAGATA+AGG 0.537235 1_2:-54893900 Msa0077870:CDS
TCAGGGCTCAAGTAGTTGGT+TGG 0.538535 1_2:-54894618 Msa0077870:intron
ACTTAGCTGGTGGCTTAGCA+GGG 0.540758 1_2:+54895376 None:intergenic
TCTTGTATTATAATGCACCT+TGG 0.543903 1_2:+54895774 None:intergenic
TTCTTCCTGTATGACCTCAC+TGG 0.545123 1_2:+54894592 None:intergenic
AAGGTAAGGTGCTCCATCCA+TGG 0.548189 1_2:+54894486 None:intergenic
TGCGAATTTCACATCATGCT+AGG 0.551834 1_2:+54895888 None:intergenic
AAGTCAACCTTACGAAGGTA+AGG 0.557851 1_2:+54894472 None:intergenic
TGCTCGTGGGAGATGTCCCA+TGG 0.560169 1_2:-54893865 Msa0077870:CDS
TGGAGCACCTTACCTTCGTA+AGG 0.564503 1_2:-54894479 Msa0077870:CDS
GTGTTGATGTTTCTCCTCAA+GGG 0.566405 1_2:+54895744 None:intergenic
CCAAGGGCTTCTGCGAAGAG+AGG 0.567370 1_2:-54895492 Msa0077870:CDS
CATTTCAGCACCAAGAGCAA+TGG 0.570918 1_2:-54892994 Msa0077870:intron
GTTCTGAGGTGGAAACTCCA+AGG 0.573378 1_2:-54895509 Msa0077870:CDS
ATGTTCAGCTCCTTCACCAC+TGG 0.578252 1_2:-54894403 Msa0077870:intron
ATTAACTTATATTATTGAGG+GGG 0.578604 1_2:-54895839 Msa0077870:five_prime_UTR
TAATTAATTACCAGTGGTGA+AGG 0.579528 1_2:+54894393 None:intergenic
AATATGGCCACTATGGGGCA+TGG 0.587296 1_2:-54895600 Msa0077870:CDS
CTTAGCAGGGTCCTTAAGGA+GGG 0.587502 1_2:+54895389 None:intergenic
GTTCAATCCATGCCCCATAG+TGG 0.588585 1_2:+54895593 None:intergenic
CGGTGGAGGAGGTTCTGAGG+TGG 0.591005 1_2:-54895520 Msa0077870:CDS
ATCGAAGTCCCGTACCAAAG+TGG 0.594032 1_2:-54892916 Msa0077870:three_prime_UTR
AAGCGTCTGCGCATAATGAA+AGG 0.596352 1_2:-54893642 Msa0077870:CDS
GGATGGCGACTGGATGCTCG+TGG 0.596688 1_2:-54893879 Msa0077870:CDS
TTCTGAGGTGGAAACTCCAA+GGG 0.597358 1_2:-54895508 Msa0077870:CDS
ATGAAGATAAGGATGGCGAC+TGG 0.598557 1_2:-54893889 Msa0077870:CDS
GCTATAGGAACTTACTCCCA+TGG 0.598945 1_2:+54893848 None:intergenic
AATTAACTTATATTATTGAG+GGG 0.603113 1_2:-54895840 Msa0077870:five_prime_UTR
GGGAAAACTTACTTAGCTGG+TGG 0.604797 1_2:+54895366 None:intergenic
CGGCGGTGGAGGAGGTTCTG+AGG 0.606000 1_2:-54895523 Msa0077870:CDS
TTCCTGTATGACCTCACTGG+TGG 0.606534 1_2:+54894595 None:intergenic
ATAGGTAACTATGGGGCCCA+AGG 0.608548 1_2:-54893980 Msa0077870:intron
GTACGGGACTTCGATATAGG+AGG 0.608615 1_2:+54892924 None:intergenic
GAAGCTTAATCTTCAAACCA+AGG 0.609730 1_2:-54895448 Msa0077870:CDS
CTTTGTCTTGGTCTGCCTGG+TGG 0.610253 1_2:-54895555 Msa0077870:CDS
AAGGTGCATTATAATACAAG+AGG 0.617418 1_2:-54895772 Msa0077870:five_prime_UTR
CTATAGGAACTTACTCCCAT+GGG 0.622755 1_2:+54893849 None:intergenic
GGGGCATGGATTGAACCTGA+AGG 0.627746 1_2:-54895586 Msa0077870:CDS
CCAACCTATGAAGATAAGGA+TGG 0.646142 1_2:-54893896 Msa0077870:CDS
GCTCGTGGGAGATGTCCCAT+GGG 0.650152 1_2:-54893864 Msa0077870:CDS
AAACTACAATAGTATATAGG+TGG 0.655587 1_2:+54892751 None:intergenic
GTCATACAGGAAGAACATGA+TGG 0.666998 1_2:-54894584 Msa0077870:CDS
GATGGCGACTGGATGCTCGT+GGG 0.667120 1_2:-54893878 Msa0077870:CDS
AAAAGAATATGGCCACTATG+GGG 0.689548 1_2:-54895605 Msa0077870:exon
TAGTTGGTTGGCCACCAGTG+AGG 0.691007 1_2:-54894606 Msa0077870:CDS
TACAAGAGGACACACCCTTG+AGG 0.691238 1_2:-54895758 Msa0077870:five_prime_UTR
ACACAACAAACATGACAGCA+TGG 0.695455 1_2:+54892694 None:intergenic
ATTTCTATATAGCCAAACCA+AGG 0.701377 1_2:-54895791 Msa0077870:five_prime_UTR
ATAATAGTAATAATTAAACA+AGG 0.703492 1_2:+54892727 None:intergenic
ACAAAAGGTTAACAATACTG+AGG 0.707970 1_2:-54894560 Msa0077870:CDS
TTGTCAAGTCAACCTTACGA+AGG 0.716471 1_2:+54894467 None:intergenic
ATATATTATAGGTAACTATG+GGG 0.717921 1_2:-54893987 Msa0077870:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTAATATTATTATA+AGG - chr1_2:54894388-54894407 Msa0077870:intron 0.0%
!! TATTAATTTATATATATTAT+AGG - chr1_2:54894531-54894550 Msa0077870:CDS 0.0%
!!! TTTTTTTTTTATAACTTTCT+TGG - chr1_2:54893217-54893236 Msa0077870:intron 10.0%
!!! ATTATTAATTTGAAGAAAAA+TGG + chr1_2:54893747-54893766 None:intergenic 10.0%
!! TTAAAAAATTGCATAAAATT+TGG + chr1_2:54893825-54893844 None:intergenic 10.0%
!! ATAATAGTAATAATTAAACA+AGG + chr1_2:54895805-54895824 None:intergenic 10.0%
!! TCAATTAACTTATATTATTG+AGG - chr1_2:54892687-54892706 Msa0077870:three_prime_UTR 15.0%
!! CAATTAACTTATATTATTGA+GGG - chr1_2:54892688-54892707 Msa0077870:three_prime_UTR 15.0%
!! AATTAACTTATATTATTGAG+GGG - chr1_2:54892689-54892708 Msa0077870:three_prime_UTR 15.0%
!! AATATTAAAAAGCGATATAA+GGG + chr1_2:54893517-54893536 None:intergenic 15.0%
!! AAAGAATAATTGAGATTTAT+AGG - chr1_2:54893857-54893876 Msa0077870:intron 15.0%
!! AAGAATAATTGAGATTTATA+GGG - chr1_2:54893858-54893877 Msa0077870:intron 15.0%
!! TAGATTAATTATTATCATCT+TGG - chr1_2:54894461-54894480 Msa0077870:CDS 15.0%
!! AGATTAATTATTATCATCTT+GGG - chr1_2:54894462-54894481 Msa0077870:CDS 15.0%
!! ATATATATTATAGGTAACTA+TGG - chr1_2:54894540-54894559 Msa0077870:CDS 15.0%
!! TATATATTATAGGTAACTAT+GGG - chr1_2:54894541-54894560 Msa0077870:CDS 15.0%
!!! TGGTTATTTAAATTTTTTGA+AGG - chr1_2:54894846-54894865 Msa0077870:intron 15.0%
!!! TTCAATGATTTATTTTTTGT+TGG + chr1_2:54895143-54895162 None:intergenic 15.0%
!! ATGATAATTTAGATATTTGA+AGG + chr1_2:54895285-54895304 None:intergenic 15.0%
!! AAATCATATTTACATTATGT+TGG + chr1_2:54895335-54895354 None:intergenic 15.0%
!! TGTAAATATGATTTAATAGT+TGG - chr1_2:54895341-54895360 Msa0077870:intron 15.0%
!! ATTAACTTATATTATTGAGG+GGG - chr1_2:54892690-54892709 Msa0077870:three_prime_UTR 20.0%
!!! TTGTTTAGTAAATGTTGTTT+TGG + chr1_2:54893274-54893293 None:intergenic 20.0%
!! AAAAAATAGAGAGAGAAAAA+AGG + chr1_2:54893393-54893412 None:intergenic 20.0%
!!! TTTCTTTTCTATCTTCTTAT+TGG + chr1_2:54893423-54893442 None:intergenic 20.0%
!! AATAAGAAGATAGAAAAGAA+AGG - chr1_2:54893422-54893441 Msa0077870:intron 20.0%
!! TATACTATTTGTATATGAGT+TGG - chr1_2:54893486-54893505 Msa0077870:intron 20.0%
!! ATATTAAAAAGCGATATAAG+GGG + chr1_2:54893516-54893535 None:intergenic 20.0%
!! CAATATTAAAAAGCGATATA+AGG + chr1_2:54893518-54893537 None:intergenic 20.0%
!! AATTTACTAAACAAGCATTA+TGG - chr1_2:54893559-54893578 Msa0077870:intron 20.0%
!!! TTTTTTTCTATCTTCCATTT+GGG + chr1_2:54893594-54893613 None:intergenic 20.0%
!!! TTTTTTTTCTATCTTCCATT+TGG + chr1_2:54893595-54893614 None:intergenic 20.0%
!! AATATTACAAATACAAGCAT+AGG + chr1_2:54894180-54894199 None:intergenic 20.0%
!! ATAAACTATAACAAATAGCT+AGG + chr1_2:54894315-54894334 None:intergenic 20.0%
!! ATATATTATAGGTAACTATG+GGG - chr1_2:54894542-54894561 Msa0077870:CDS 20.0%
!! AGTACATATATATGTTTGTT+GGG + chr1_2:54894779-54894798 None:intergenic 20.0%
!! AAGTACATATATATGTTTGT+TGG + chr1_2:54894780-54894799 None:intergenic 20.0%
!! AACAAACATATATATGTACT+TGG - chr1_2:54894779-54894798 Msa0077870:intron 20.0%
!! AATAACCAATGTTAATTTCA+TGG + chr1_2:54894834-54894853 None:intergenic 20.0%
!!! AATATTTTGTACTTAACTCA+TGG - chr1_2:54894962-54894981 Msa0077870:intron 20.0%
!!! TTTATTTTTTGTTGGTCTTA+AGG + chr1_2:54895135-54895154 None:intergenic 20.0%
!! ATTGAATTGAAAATCTTAGA+TGG - chr1_2:54895157-54895176 Msa0077870:intron 20.0%
!!! CTTCATATATCTTTTCTTAT+AGG + chr1_2:54895640-54895659 None:intergenic 20.0%
!!! TTTTCAGTTTTTTCATAGAG+AGG - chr1_2:54892811-54892830 Msa0077870:three_prime_UTR 25.0%
!!! TTTCAGTTTTTTCATAGAGA+GGG - chr1_2:54892812-54892831 Msa0077870:three_prime_UTR 25.0%
! TCAAAATTACAAGCATTTGT+GGG + chr1_2:54893700-54893719 None:intergenic 25.0%
! ATCAAAATTACAAGCATTTG+TGG + chr1_2:54893701-54893720 None:intergenic 25.0%
!!! TTATGCAATTTTTTAAGCCT+TGG - chr1_2:54893829-54893848 Msa0077870:intron 25.0%
! TAGTACTAATTAATTACCAG+TGG + chr1_2:54894145-54894164 None:intergenic 25.0%
!!! ACTTTTTTTTGTTGTGATGA+TGG + chr1_2:54894367-54894386 None:intergenic 25.0%
!!! TATAAGGTTTTAGATCTTGA+AGG - chr1_2:54894404-54894423 Msa0077870:CDS 25.0%
!! GTACATATATATGTTTGTTG+GGG + chr1_2:54894778-54894797 None:intergenic 25.0%
!!! ATTTTTTGAAGGATGTTTGT+TGG - chr1_2:54894857-54894876 Msa0077870:intron 25.0%
! GGCTATTTATTCTAACTTAT+TGG - chr1_2:54895495-54895514 Msa0077870:CDS 25.0%
! AAACTACAATAGTATATAGG+TGG + chr1_2:54895781-54895800 None:intergenic 25.0%
ATTGAAGAACTTTGTGTTTG+TGG + chr1_2:54892672-54892691 None:intergenic 30.0%
ATTTCTATATAGCCAAACCA+AGG - chr1_2:54892738-54892757 Msa0077870:three_prime_UTR 30.0%
TATTATAATGCACCTTGGTT+TGG + chr1_2:54892753-54892772 None:intergenic 30.0%
TCTTGTATTATAATGCACCT+TGG + chr1_2:54892758-54892777 None:intergenic 30.0%
AAGGTGCATTATAATACAAG+AGG - chr1_2:54892757-54892776 Msa0077870:three_prime_UTR 30.0%
AATCTTCTTCTTCTAAGCTT+TGG - chr1_2:54892880-54892899 Msa0077870:three_prime_UTR 30.0%
AAAAAGAATATGGCCACTAT+GGG - chr1_2:54892923-54892942 Msa0077870:three_prime_UTR 30.0%
AGCATGCAAGTACTTTAATT+GGG + chr1_2:54893186-54893205 None:intergenic 30.0%
AAGCATGCAAGTACTTTAAT+TGG + chr1_2:54893187-54893206 None:intergenic 30.0%
ATATTTGTACAACTGTTCTG+TGG - chr1_2:54893318-54893337 Msa0077870:intron 30.0%
! CAATTATTCTTTTCTCTCCA+AGG + chr1_2:54893849-54893868 None:intergenic 30.0%
ACAAAAGGTTAACAATACTG+AGG - chr1_2:54893969-54893988 Msa0077870:CDS 30.0%
TAATTAATTACCAGTGGTGA+AGG + chr1_2:54894139-54894158 None:intergenic 30.0%
CTATAACAAATAGCTAGGTA+TGG + chr1_2:54894310-54894329 None:intergenic 30.0%
TGATTGTAGTATATAGTAGC+TGG + chr1_2:54894724-54894743 None:intergenic 30.0%
TTCATGGATGTGTAAATATG+TGG + chr1_2:54894818-54894837 None:intergenic 30.0%
CACATCCATGAAATTAACAT+TGG - chr1_2:54894826-54894845 Msa0077870:intron 30.0%
! TGGTCTTAAGGATTAAGTTT+AGG + chr1_2:54895123-54895142 None:intergenic 30.0%
ATTTAGACCAAAATCAGCTT+CGG + chr1_2:54895574-54895593 None:intergenic 30.0%
GGCAAACTACAATAGTATAT+AGG + chr1_2:54895784-54895803 None:intergenic 30.0%
GAAGCTAGCCAAAAAGAATA+TGG - chr1_2:54892913-54892932 Msa0077870:three_prime_UTR 35.0%
! CATAGTGGCCATATTCTTTT+TGG + chr1_2:54892924-54892943 None:intergenic 35.0%
CAAAAAGAATATGGCCACTA+TGG - chr1_2:54892922-54892941 Msa0077870:three_prime_UTR 35.0%
AAAAGAATATGGCCACTATG+GGG - chr1_2:54892924-54892943 Msa0077870:three_prime_UTR 35.0%
GAAGCTTAATCTTCAAACCA+AGG - chr1_2:54893081-54893100 Msa0077870:intron 35.0%
ATTGGGAAAACTTACTTAGC+TGG + chr1_2:54893169-54893188 None:intergenic 35.0%
ATAGAGAGAGAAAAAAGGAG+AGG + chr1_2:54893388-54893407 None:intergenic 35.0%
TATGGATTCTCAAACCCAAA+TGG - chr1_2:54893577-54893596 Msa0077870:intron 35.0%
! TGCATTTGTTAAGGTTTCCA+TGG - chr1_2:54894026-54894045 Msa0077870:intron 35.0%
TTTGTTAAGGTTTCCATGGA+TGG - chr1_2:54894030-54894049 Msa0077870:intron 35.0%
GGCATCAGATAAATCTTTGT+AGG + chr1_2:54894097-54894116 None:intergenic 35.0%
! CATTCTGTCTAGTTTAGATG+GGG + chr1_2:54894224-54894243 None:intergenic 35.0%
! GCATTCTGTCTAGTTTAGAT+GGG + chr1_2:54894225-54894244 None:intergenic 35.0%
! AGCATTCTGTCTAGTTTAGA+TGG + chr1_2:54894226-54894245 None:intergenic 35.0%
AATAGCTAGGTATGGACTTT+AGG + chr1_2:54894302-54894321 None:intergenic 35.0%
TTTAGATCTTGAAGGATGCA+TGG - chr1_2:54894412-54894431 Msa0077870:CDS 35.0%
CATGAAGTCTATCATTCCTT+GGG + chr1_2:54894568-54894587 None:intergenic 35.0%
TCATGAAGTCTATCATTCCT+TGG + chr1_2:54894569-54894588 None:intergenic 35.0%
TAGTATATAGTAGCTGGAGA+TGG + chr1_2:54894718-54894737 None:intergenic 35.0%
TGGATGTGTAAATATGTGGT+TGG + chr1_2:54894814-54894833 None:intergenic 35.0%
ATGAAAGGATCAGAAGCTAT+TGG - chr1_2:54894902-54894921 Msa0077870:intron 35.0%
TGAAAGGATCAGAAGCTATT+GGG - chr1_2:54894903-54894922 Msa0077870:intron 35.0%
! TAACTCATGGAAGTATGCTT+TGG - chr1_2:54894975-54894994 Msa0077870:intron 35.0%
TGCATCAAGTATAATGCAAG+TGG + chr1_2:54895071-54895090 None:intergenic 35.0%
! CTTTTCTTATAGGTCCACTT+TGG + chr1_2:54895630-54895649 None:intergenic 35.0%
AAACTAACACTCCAATCAAC+AGG + chr1_2:54895668-54895687 None:intergenic 35.0%
! TGTTGATTGGAGTGTTAGTT+TGG - chr1_2:54895667-54895686 Msa0077870:five_prime_UTR 35.0%
! GTGTTGATGTTTCTCCTCAA+GGG + chr1_2:54892788-54892807 None:intergenic 40.0%
! TGTGTTGATGTTTCTCCTCA+AGG + chr1_2:54892789-54892808 None:intergenic 40.0%
CTTCTCATTCAGATCTTCCT+TGG + chr1_2:54893101-54893120 None:intergenic 40.0%
!! GAATGAGAAGAGTGCTTCAA+AGG - chr1_2:54893111-54893130 Msa0077870:intron 40.0%
TATTTCAGGGCTCAAGTAGT+TGG - chr1_2:54893907-54893926 Msa0077870:CDS 40.0%
GTCATACAGGAAGAACATGA+TGG - chr1_2:54893945-54893964 Msa0077870:CDS 40.0%
GAAGAACATGATGGCACAAA+AGG - chr1_2:54893954-54893973 Msa0077870:CDS 40.0%
! TACTGCTGCTGCATTTGTTA+AGG - chr1_2:54894017-54894036 Msa0077870:intron 40.0%
AAGTCAACCTTACGAAGGTA+AGG + chr1_2:54894060-54894079 None:intergenic 40.0%
TTGTCAAGTCAACCTTACGA+AGG + chr1_2:54894065-54894084 None:intergenic 40.0%
! CATGAATGAGAGCAAGTTGA+TGG - chr1_2:54894584-54894603 Msa0077870:CDS 40.0%
TGTGCCAACCTATGAAGATA+AGG - chr1_2:54894629-54894648 Msa0077870:intron 40.0%
CCATCCTTATCTTCATAGGT+TGG + chr1_2:54894636-54894655 None:intergenic 40.0%
CCAACCTATGAAGATAAGGA+TGG - chr1_2:54894633-54894652 Msa0077870:intron 40.0%
CTATAGGAACTTACTCCCAT+GGG + chr1_2:54894683-54894702 None:intergenic 40.0%
TATATAGTAGCTGGAGATGG+AGG + chr1_2:54894715-54894734 None:intergenic 40.0%
ACAAAATGACACGCACACAA+GGG + chr1_2:54895475-54895494 None:intergenic 40.0%
TTGCATTTCTCCATTGCTCT+TGG + chr1_2:54895548-54895567 None:intergenic 40.0%
!! GCAAAAACCGAAGCTGATTT+TGG - chr1_2:54895564-54895583 Msa0077870:CDS 40.0%
TTATAGGTCCACTTTGGTAC+GGG + chr1_2:54895624-54895643 None:intergenic 40.0%
CTTATAGGTCCACTTTGGTA+CGG + chr1_2:54895625-54895644 None:intergenic 40.0%
! ATGAAGCACTACCTGTTGAT+TGG - chr1_2:54895654-54895673 Msa0077870:five_prime_UTR 40.0%
ACACAACAAACATGACAGCA+TGG + chr1_2:54895838-54895857 None:intergenic 40.0%
! AAGGAGACTGAGCTTTGTCT+TGG - chr1_2:54892962-54892981 Msa0077870:exon 45.0%
!! TTCTGAGGTGGAAACTCCAA+GGG - chr1_2:54893021-54893040 Msa0077870:intron 45.0%
AAAGGAGAAGACCCTCCTTA+AGG - chr1_2:54893129-54893148 Msa0077870:intron 45.0%
GGGAAAACTTACTTAGCTGG+TGG + chr1_2:54893166-54893185 None:intergenic 45.0%
TTCTTCCTGTATGACCTCAC+TGG + chr1_2:54893940-54893959 None:intergenic 45.0%
!!! GGAGCTGAACATTTTGGCTA+AGG + chr1_2:54894118-54894137 None:intergenic 45.0%
! GGTGAAGGAGCTGAACATTT+TGG + chr1_2:54894124-54894143 None:intergenic 45.0%
GTCGCCATCCTTATCTTCAT+AGG + chr1_2:54894640-54894659 None:intergenic 45.0%
ATGAAGATAAGGATGGCGAC+TGG - chr1_2:54894640-54894659 Msa0077870:intron 45.0%
GCTATAGGAACTTACTCCCA+TGG + chr1_2:54894684-54894703 None:intergenic 45.0%
ATGGAGGTTGGATGAGCTAT+AGG + chr1_2:54894699-54894718 None:intergenic 45.0%
AAGCGTCTGCGCATAATGAA+AGG - chr1_2:54894887-54894906 Msa0077870:intron 45.0%
AAAATGACACGCACACAAGG+GGG + chr1_2:54895473-54895492 None:intergenic 45.0%
CAAAATGACACGCACACAAG+GGG + chr1_2:54895474-54895493 None:intergenic 45.0%
CACAAAATGACACGCACACA+AGG + chr1_2:54895476-54895495 None:intergenic 45.0%
!!! CTTGTGTGCGTGTCATTTTG+TGG - chr1_2:54895474-54895493 Msa0077870:CDS 45.0%
CATTTCAGCACCAAGAGCAA+TGG - chr1_2:54895535-54895554 Msa0077870:CDS 45.0%
TTGGTACGGGACTTCGATAT+AGG + chr1_2:54895611-54895630 None:intergenic 45.0%
!! TATTATATTAATAATATTTC+AGG - chr1_2:54893893-54893912 Msa0077870:CDS 5.0%
!! ATTATATTAATAATATTTCA+GGG - chr1_2:54893894-54893913 Msa0077870:CDS 5.0%
!! ATTTGTAATATTTATATATA+TGG - chr1_2:54894188-54894207 Msa0077870:intron 5.0%
TACAAGAGGACACACCCTTG+AGG - chr1_2:54892771-54892790 Msa0077870:three_prime_UTR 50.0%
AATATGGCCACTATGGGGCA+TGG - chr1_2:54892929-54892948 Msa0077870:three_prime_UTR 50.0%
GTTCAATCCATGCCCCATAG+TGG + chr1_2:54892939-54892958 None:intergenic 50.0%
GACAAAGCTCAGTCTCCTTC+AGG + chr1_2:54892961-54892980 None:intergenic 50.0%
!! GTTCTGAGGTGGAAACTCCA+AGG - chr1_2:54893020-54893039 Msa0077870:intron 50.0%
CTTAGCAGGGTCCTTAAGGA+GGG + chr1_2:54893143-54893162 None:intergenic 50.0%
ACTTAGCTGGTGGCTTAGCA+GGG + chr1_2:54893156-54893175 None:intergenic 50.0%
TACTTAGCTGGTGGCTTAGC+AGG + chr1_2:54893157-54893176 None:intergenic 50.0%
TCAGGGCTCAAGTAGTTGGT+TGG - chr1_2:54893911-54893930 Msa0077870:CDS 50.0%
TTCCTGTATGACCTCACTGG+TGG + chr1_2:54893937-54893956 None:intergenic 50.0%
! AAGGTAAGGTGCTCCATCCA+TGG + chr1_2:54894046-54894065 None:intergenic 50.0%
! TGGAGCACCTTACCTTCGTA+AGG - chr1_2:54894050-54894069 Msa0077870:intron 50.0%
ATGTTCAGCTCCTTCACCAC+TGG - chr1_2:54894126-54894145 Msa0077870:intron 50.0%
AGGATGCATGGCGATTGTTG+AGG - chr1_2:54894424-54894443 Msa0077870:CDS 50.0%
ATAGGTAACTATGGGGCCCA+AGG - chr1_2:54894549-54894568 Msa0077870:CDS 50.0%
TAGTAGCTGGAGATGGAGGT+TGG + chr1_2:54894711-54894730 None:intergenic 50.0%
GGATCAGAAGCTATTGGGCT+TGG - chr1_2:54894908-54894927 Msa0077870:intron 50.0%
AAATGACACGCACACAAGGG+GGG + chr1_2:54895472-54895491 None:intergenic 50.0%
GTACGGGACTTCGATATAGG+AGG + chr1_2:54895608-54895627 None:intergenic 50.0%
ATCGAAGTCCCGTACCAAAG+TGG - chr1_2:54895613-54895632 Msa0077870:exon 50.0%
! GGGGCATGGATTGAACCTGA+AGG - chr1_2:54892943-54892962 Msa0077870:three_prime_UTR 55.0%
! GAGCTTTGTCTTGGTCTGCC+TGG - chr1_2:54892971-54892990 Msa0077870:CDS 55.0%
CTTTGTCTTGGTCTGCCTGG+TGG - chr1_2:54892974-54892993 Msa0077870:CDS 55.0%
GCTTAGCAGGGTCCTTAAGG+AGG + chr1_2:54893144-54893163 None:intergenic 55.0%
GTGGCTTAGCAGGGTCCTTA+AGG + chr1_2:54893147-54893166 None:intergenic 55.0%
! TAGTTGGTTGGCCACCAGTG+AGG - chr1_2:54893923-54893942 Msa0077870:CDS 55.0%
TGTCTTGGTCTGCCTGGTGG+TGG - chr1_2:54892977-54892996 Msa0077870:CDS 60.0%
CCTCTCTTCGCAGAAGCCCT+TGG + chr1_2:54893040-54893059 None:intergenic 60.0%
CCAAGGGCTTCTGCGAAGAG+AGG - chr1_2:54893037-54893056 Msa0077870:intron 60.0%
GGCCACCAGTGAGGTCATAC+AGG - chr1_2:54893932-54893951 Msa0077870:CDS 60.0%
GATGGCGACTGGATGCTCGT+GGG - chr1_2:54894651-54894670 Msa0077870:intron 60.0%
TGCTCGTGGGAGATGTCCCA+TGG - chr1_2:54894664-54894683 Msa0077870:intron 60.0%
GCTCGTGGGAGATGTCCCAT+GGG - chr1_2:54894665-54894684 Msa0077870:intron 60.0%
GCACACAAGGGGGGACTCAA+GGG + chr1_2:54895463-54895482 None:intergenic 60.0%
!! CTTGGTCTGCCTGGTGGTGG+TGG - chr1_2:54892980-54892999 Msa0077870:CDS 65.0%
!! CGGTGGAGGAGGTTCTGAGG+TGG - chr1_2:54893009-54893028 Msa0077870:intron 65.0%
GGATGGCGACTGGATGCTCG+TGG - chr1_2:54894650-54894669 Msa0077870:intron 65.0%
CGCACACAAGGGGGGACTCA+AGG + chr1_2:54895464-54895483 None:intergenic 65.0%
!! GGTCTGCCTGGTGGTGGTGG+CGG - chr1_2:54892983-54893002 Msa0077870:CDS 70.0%
CGGCGGTGGAGGAGGTTCTG+AGG - chr1_2:54893006-54893025 Msa0077870:intron 70.0%
!! CTGCCTGGTGGTGGTGGCGG+CGG - chr1_2:54892986-54893005 Msa0077870:CDS 75.0%
!!! CCTGGTGGTGGTGGCGGCGG+CGG - chr1_2:54892989-54893008 Msa0077870:CDS 80.0%
! CCGCCGCCGCCACCACCACC+AGG + chr1_2:54892992-54893011 None:intergenic 85.0%
!!! GGTGGTGGTGGCGGCGGCGG+TGG - chr1_2:54892992-54893011 Msa0077870:intron 85.0%
!!! GGTGGTGGCGGCGGCGGTGG+AGG - chr1_2:54892995-54893014 Msa0077870:intron 85.0%
!! GGTGGCGGCGGCGGTGGAGG+AGG - chr1_2:54892998-54893017 Msa0077870:intron 85.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54892644 54895907 54892644 ID=Msa0077870;Name=Msa0077870
chr1_2 mRNA 54892644 54895907 54892644 ID=Msa0077870-mRNA-1;Parent=Msa0077870;Name=Msa0077870-mRNA-1;_AED=0.04;_eAED=0.04;_QI=288|1|1|1|1|1|5|327|236
chr1_2 exon 54892644 54893008 54892644 ID=Msa0077870-mRNA-1:exon:17119;Parent=Msa0077870-mRNA-1
chr1_2 exon 54893622 54893683 54893622 ID=Msa0077870-mRNA-1:exon:17118;Parent=Msa0077870-mRNA-1
chr1_2 exon 54893863 54893998 54893863 ID=Msa0077870-mRNA-1:exon:17117;Parent=Msa0077870-mRNA-1
chr1_2 exon 54894404 54894636 54894404 ID=Msa0077870-mRNA-1:exon:17116;Parent=Msa0077870-mRNA-1
chr1_2 exon 54895378 54895907 54895378 ID=Msa0077870-mRNA-1:exon:17115;Parent=Msa0077870-mRNA-1
chr1_2 five_prime_UTR 54895620 54895907 54895620 ID=Msa0077870-mRNA-1:five_prime_utr;Parent=Msa0077870-mRNA-1
chr1_2 CDS 54895378 54895619 54895378 ID=Msa0077870-mRNA-1:cds;Parent=Msa0077870-mRNA-1
chr1_2 CDS 54894404 54894636 54894404 ID=Msa0077870-mRNA-1:cds;Parent=Msa0077870-mRNA-1
chr1_2 CDS 54893863 54893998 54893863 ID=Msa0077870-mRNA-1:cds;Parent=Msa0077870-mRNA-1
chr1_2 CDS 54893622 54893683 54893622 ID=Msa0077870-mRNA-1:cds;Parent=Msa0077870-mRNA-1
chr1_2 CDS 54892971 54893008 54892971 ID=Msa0077870-mRNA-1:cds;Parent=Msa0077870-mRNA-1
chr1_2 three_prime_UTR 54892644 54892970 54892644 ID=Msa0077870-mRNA-1:three_prime_utr;Parent=Msa0077870-mRNA-1
Gene Sequence

>Msa0077870

ATGGCCACTATGGGGCATGGATTGAACCTGAAGGAGACTGAGCTTTGTCTTGGTCTGCCTGGTGGTGGTGGCGGCGGCGGTGGAGGAGGTTCTGAGGTGGAAACTCCAAGGGCTTCTGCGAAGAGAGGTTTCTCTGAGACTGTTGATCTGAAGCTTAATCTTCAAACCAAGGAAGATCTGAATGAGAAGAGTGCTTCAAAGGAGAAGACCCTCCTTAAGGACCCTGCTAAGCCACCAGCTAAGGCTCAAGTAGTTGGTTGGCCACCAGTGAGGTCATACAGGAAGAACATGATGGCACAAAAGGTTAACAATACTGAGGACACTGAGAAGACAACAAGTAACACTACTGCTGCTGCATTTGTTAAGGTTTCCATGGATGGAGCACCTTACCTTCGTAAGGTTGACTTGACAATGTACAAAACCTACAAAGATTTATCTGATGCCTTAGCCAAAATGTTCAGCTCCTTCACCACTGGTAACTATGGGGCCCAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTTAACAGCTCTGAGTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGAGATGTCCCATGGGAGATGTTTGTTGGATCATGCAAGCGTCTGCGCATAATGAAAGGATCAGAAGCTATTGGGCTTGCACCAAGAGCAATGGAGAAATGCAAAAACCGAAGCTGA
Protein sequence

>Msa0077870

MATMGHGLNLKETELCLGLPGGGGGGGGGGSEVETPRASAKRGFSETVDLKLNLQTKEDLNEKSASKEKTLLKDPAKPPAKAQVVGWPPVRSYRKNMMAQKVNNTEDTEKTTSNTTAAAFVKVSMDGAPYLRKVDLTMYKTYKDLSDALAKMFSSFTTGNYGAQGMIDFMNESKLMDLLNSSEYVPTYEDKDGDWMLVGDVPWEMFVGSCKRLRIMKGSEAIGLAPRAMEKCKNRS*