AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0125760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125760 MtrunA17_Chr1g0187921 98.739 238 1 1 1 238 1 236 3.31e-174 478
Msa0125760 MtrunA17_Chr1g0184791 68.050 241 51 7 12 238 17 245 5.74e-102 296
Msa0125760 MtrunA17_Chr7g0260641 63.200 250 67 5 6 238 9 250 1.66e-97 285
Msa0125760 MtrunA17_Chr1g0190711 62.602 246 70 6 8 238 15 253 4.87e-96 281
Msa0125760 MtrunA17_Chr6g0486121 51.362 257 86 6 6 238 13 254 5.09e-79 238
Msa0125760 MtrunA17_Chr8g0390281 48.561 278 90 9 7 238 23 293 1.94e-70 217
Msa0125760 MtrunA17_Chr5g0411501 67.702 161 49 2 80 238 176 335 2.40e-70 218
Msa0125760 MtrunA17_Chr8g0366881 45.105 286 102 6 6 238 65 348 9.86e-68 212
Msa0125760 MtrunA17_Chr5g0428141 62.893 159 57 2 80 238 191 347 3.33e-66 208
Msa0125760 MtrunA17_Chr2g0331411 43.894 303 97 8 8 238 25 326 4.42e-66 207
Msa0125760 MtrunA17_Chr1g0184801 46.119 219 89 4 8 226 12 201 1.00e-53 172
Msa0125760 MtrunA17_Chr7g0260621 45.000 220 93 5 8 227 11 202 5.38e-52 167
Msa0125760 MtrunA17_Chr1g0187961 45.662 219 68 6 8 226 11 178 1.26e-51 165
Msa0125760 MtrunA17_Chr1g0166011 44.292 219 85 5 8 226 1 182 4.52e-50 162
Msa0125760 MtrunA17_Chr4g0004181 41.126 231 74 7 1 229 1 171 4.04e-38 131
Msa0125760 MtrunA17_Chr1g0183251 42.623 183 72 3 84 233 81 263 1.87e-37 132
Msa0125760 MtrunA17_Chr2g0332141 53.846 117 43 4 118 230 4 113 7.11e-35 120
Msa0125760 MtrunA17_Chr8g0340731 37.714 175 89 3 66 222 88 260 3.31e-29 110
Msa0125760 MtrunA17_Chr2g0330951 38.824 170 73 5 79 222 90 254 4.88e-28 107
Msa0125760 MtrunA17_Chr4g0069561 32.867 143 67 4 83 223 69 184 2.58e-19 82.4
Msa0125760 MtrunA17_Chr1g0193861 41.509 106 48 3 115 219 78 170 4.53e-17 76.3
Msa0125760 MtrunA17_Chr3g0137601 26.950 141 83 3 85 223 88 210 4.99e-17 77.0
Msa0125760 MtrunA17_Chr4g0064131 26.667 135 78 1 86 220 93 206 3.44e-15 72.0
Msa0125760 MtrunA17_Chr7g0270851 39.604 101 47 4 120 219 68 155 2.22e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125760 AT4G14550.1 74.370 238 44 4 8 238 1 228 2.56e-120 342
Msa0125760 AT3G23050.1 70.518 251 49 6 4 238 2 243 3.53e-117 335
Msa0125760 AT4G14550.2 73.451 226 43 4 8 226 1 216 5.34e-112 321
Msa0125760 AT1G04250.1 64.730 241 57 5 8 238 7 229 4.99e-103 298
Msa0125760 AT3G04730.1 63.525 244 67 4 8 238 2 236 3.45e-102 296
Msa0125760 AT4G14550.3 71.707 205 41 4 8 205 1 195 2.25e-97 283
Msa0125760 AT3G23050.2 67.431 218 47 5 4 206 2 210 1.89e-94 276
Msa0125760 AT4G29080.1 48.699 269 101 8 6 237 36 304 8.14e-71 219
Msa0125760 AT5G65670.2 52.055 219 80 5 43 238 120 336 3.72e-66 208
Msa0125760 AT5G65670.1 51.584 221 80 6 43 238 120 338 4.06e-64 203
Msa0125760 AT2G22670.3 52.804 214 80 5 43 237 107 318 5.27e-63 199
Msa0125760 AT2G22670.1 52.804 214 80 5 43 237 107 318 5.27e-63 199
Msa0125760 AT2G22670.4 52.804 214 80 5 43 237 124 335 9.02e-63 199
Msa0125760 AT4G14550.4 65.839 161 38 4 8 161 1 151 5.35e-62 192
Msa0125760 AT2G22670.5 51.869 214 80 6 43 237 107 316 1.06e-59 191
Msa0125760 AT2G22670.2 51.869 214 80 6 43 237 107 316 1.06e-59 191
Msa0125760 AT3G23050.3 60.000 175 46 5 4 163 2 167 3.38e-59 184
Msa0125760 AT1G04240.1 47.085 223 80 7 4 226 1 185 1.41e-54 174
Msa0125760 AT1G04240.2 46.460 226 83 7 1 226 98 285 1.20e-53 174
Msa0125760 AT5G43700.1 47.945 219 66 7 8 226 11 181 3.76e-52 167
Msa0125760 AT3G23030.1 44.749 219 64 6 8 226 9 170 7.84e-49 159
Msa0125760 AT3G23030.2 44.749 219 64 6 8 226 96 257 4.61e-48 159
Msa0125760 AT1G15580.1 40.271 221 70 4 1 221 1 159 3.48e-44 146
Msa0125760 AT4G14560.1 42.661 218 67 5 6 223 5 164 1.65e-43 145
Msa0125760 AT3G15540.1 39.744 234 95 7 6 233 4 197 2.58e-40 137
Msa0125760 AT2G33310.3 43.353 173 76 4 78 232 68 236 5.64e-39 135
Msa0125760 AT2G33310.1 43.353 173 76 4 78 232 68 236 5.64e-39 135
Msa0125760 AT2G33310.2 44.767 172 71 6 80 232 71 237 7.33e-38 132
Msa0125760 AT1G52830.1 38.914 221 95 6 6 226 4 184 1.62e-37 130
Msa0125760 AT4G28640.1 48.408 157 65 5 85 227 88 242 2.15e-35 126
Msa0125760 AT4G28640.3 48.387 155 64 5 85 225 88 240 3.38e-35 126
Msa0125760 AT1G04550.2 41.477 176 76 3 78 233 62 230 3.94e-35 125
Msa0125760 AT4G28640.2 48.387 155 64 5 85 225 88 240 7.32e-34 124
Msa0125760 AT3G16500.1 37.768 233 108 8 11 222 36 252 1.22e-33 122
Msa0125760 AT3G16500.2 37.768 233 108 8 11 222 36 252 1.55e-33 122
Msa0125760 AT5G25890.1 39.157 166 77 3 64 229 29 170 3.54e-30 110
Msa0125760 AT1G04100.1 40.719 167 70 4 87 226 95 259 7.20e-30 112
Msa0125760 AT1G51950.1 40.625 160 74 4 82 222 93 250 2.73e-28 108
Msa0125760 AT3G17600.1 32.877 146 68 4 77 219 36 154 2.69e-21 87.4
Msa0125760 AT2G46990.1 39.623 106 51 2 116 221 80 172 8.11e-19 81.3
Msa0125760 AT3G62100.1 38.889 108 53 2 112 219 74 168 1.73e-18 80.1
Msa0125760 AT2G46990.2 40.659 91 41 2 116 206 80 157 4.29e-14 68.2
Msa0125760 AT4G32280.1 23.944 213 105 6 52 234 64 249 1.25e-13 68.6
Msa0125760 AT3G62100.2 37.895 95 46 2 112 206 74 155 2.28e-13 66.2
Msa0125760 AT1G80390.1 33.553 152 61 3 9 160 15 126 4.00e-13 64.7
Msa0125760 AT4G32280.2 29.204 113 56 2 122 234 119 207 1.48e-12 65.1

Find 105 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAAGGAGCTGAACATTT+TGG 0.275554 1_3:+54349426 None:intergenic
GGTGGTGGTGGCGGCGGCGG+CGG 0.285511 1_3:-54350557 Msa0125760:CDS
ATATATATTATAGGTAACTA+TGG 0.289007 1_3:-54349006 Msa0125760:intron
AATCTTCTTCTTCTAAGCTT+TGG 0.317444 1_3:-54350669 Msa0125760:five_prime_UTR
ATTGAAGAACTTTGTGTTTG+TGG 0.337731 1_3:+54350881 None:intergenic
GGCGGCGGCGGCGGTGGAGG+TGG 0.338430 1_3:-54350545 Msa0125760:CDS
CTTCTCATTCAGATCTTCCT+TGG 0.341646 1_3:+54350445 None:intergenic
GGTGGTGGCGGCGGCGGCGG+CGG 0.347006 1_3:-54350554 Msa0125760:CDS
TACTTAGCTGGTGGCTTAGC+AGG 0.347183 1_3:+54350389 None:intergenic
TTGCATTTCTCCATTGCTCT+TGG 0.358710 1_3:+54347972 None:intergenic
TATTATAATGCACCTTGGTT+TGG 0.368189 1_3:+54350802 None:intergenic
GACAAAGCTCAGTCTCCTTC+AGG 0.370771 1_3:+54350591 None:intergenic
CCTGGTGGTGGTGGCGGCGG+CGG 0.370944 1_3:-54350560 Msa0125760:CDS
ATGAAAGGATCAGAAGCTAT+TGG 0.371063 1_3:-54348644 Msa0125760:CDS
TGTTGATTGGAGTGTTAGTT+TGG 0.376698 1_3:-54347850 Msa0125760:three_prime_UTR
GGCATCAGATAAATCTTTGT+AGG 0.380510 1_3:+54349453 None:intergenic
GTGGCTTAGCAGGGTCCTTA+AGG 0.389548 1_3:+54350399 None:intergenic
TACTGCTGCTGCATTTGTTA+AGG 0.392669 1_3:-54349530 Msa0125760:CDS
CATGAATGAGAGCAAGTTGA+TGG 0.395903 1_3:-54348962 Msa0125760:CDS
TCAATTAACTTATATTATTG+AGG 0.412560 1_3:-54350863 Msa0125760:five_prime_UTR
CTGCCTGGTGGTGGTGGCGG+CGG 0.419116 1_3:-54350563 Msa0125760:CDS
CCGCCGCCGCCACCACCACC+AGG 0.425081 1_3:+54350560 None:intergenic
TGAAAGGATCAGAAGCTATT+GGG 0.427068 1_3:-54348643 Msa0125760:CDS
ATTGGGAAAACTTACTTAGC+TGG 0.432635 1_3:+54350377 None:intergenic
CAATTAACTTATATTATTGA+GGG 0.435956 1_3:-54350862 Msa0125760:five_prime_UTR
TCATGAAGTCTATCATTCCT+TGG 0.436607 1_3:+54348980 None:intergenic
GAGCTTTGTCTTGGTCTGCC+TGG 0.443783 1_3:-54350578 Msa0125760:CDS
ATGAAGCACTACCTGTTGAT+TGG 0.446997 1_3:-54347863 Msa0125760:three_prime_UTR
TTTGTTAAGGTTTCCATGGA+TGG 0.449811 1_3:-54349517 Msa0125760:CDS
AAGGAGACTGAGCTTTGTCT+TGG 0.456647 1_3:-54350587 Msa0125760:CDS
GGATCAGAAGCTATTGGGCT+TGG 0.459856 1_3:-54348638 Msa0125760:intron
CTTGGTCTGCCTGGTGGTGG+TGG 0.460328 1_3:-54350569 Msa0125760:CDS
CATGAAGTCTATCATTCCTT+GGG 0.461454 1_3:+54348981 None:intergenic
TATTTCAGGGCTCAAGTAGT+TGG 0.461914 1_3:-54349640 Msa0125760:intron
TGTCTTGGTCTGCCTGGTGG+TGG 0.462328 1_3:-54350572 Msa0125760:CDS
TATATATTATAGGTAACTAT+GGG 0.462994 1_3:-54349005 Msa0125760:intron
AAAGGAGAAGACCCTCCTTA+AGG 0.463373 1_3:-54350414 Msa0125760:CDS
TTGGTACGGGACTTCGATAT+AGG 0.464620 1_3:+54347909 None:intergenic
GTCGCCATCCTTATCTTCAT+AGG 0.465608 1_3:+54348909 None:intergenic
GAAGAACATGATGGCACAAA+AGG 0.465863 1_3:-54349593 Msa0125760:CDS
CTTATAGGTCCACTTTGGTA+CGG 0.467220 1_3:+54347895 None:intergenic
AAACTAACACTCCAATCAAC+AGG 0.468673 1_3:+54347852 None:intergenic
CCTCTCTTCGCAGAAGCCCT+TGG 0.474958 1_3:+54350506 None:intergenic
ATTTAGACCAAAATCAGCTT+CGG 0.485181 1_3:+54347946 None:intergenic
GCTTAGCAGGGTCCTTAAGG+AGG 0.489152 1_3:+54350402 None:intergenic
TTATAGGTCCACTTTGGTAC+GGG 0.492481 1_3:+54347896 None:intergenic
GGCAAACTACAATAGTATAT+AGG 0.494578 1_3:+54347736 None:intergenic
GGCCACCAGTGAGGTCATAC+AGG 0.498221 1_3:-54349615 Msa0125760:CDS
GGTCTGCCTGGTGGTGGTGG+CGG 0.503771 1_3:-54350566 Msa0125760:CDS
TGTGTTGATGTTTCTCCTCA+AGG 0.507891 1_3:+54350763 None:intergenic
CCATCCTTATCTTCATAGGT+TGG 0.508321 1_3:+54348913 None:intergenic
CGGTGGAGGTGGTTCTGAGG+TGG 0.509616 1_3:-54350534 Msa0125760:CDS
GAATGAGAAGAGTGCTTCAA+AGG 0.512968 1_3:-54350432 Msa0125760:CDS
TGCATTTGTTAAGGTTTCCA+TGG 0.525650 1_3:-54349521 Msa0125760:CDS
TGTGCCAACCTATGAAGATA+AGG 0.537235 1_3:-54348917 Msa0125760:CDS
TCAGGGCTCAAGTAGTTGGT+TGG 0.538535 1_3:-54349636 Msa0125760:intron
ACTTAGCTGGTGGCTTAGCA+GGG 0.540758 1_3:+54350390 None:intergenic
TCTTGTATTATAATGCACCT+TGG 0.543903 1_3:+54350797 None:intergenic
TTCTTCCTGTATGACCTCAC+TGG 0.545123 1_3:+54349610 None:intergenic
AAGGTAAGGTGCTCCATCCA+TGG 0.548189 1_3:+54349504 None:intergenic
TGCGAATTTCACATCATGCT+AGG 0.551834 1_3:+54350909 None:intergenic
AAGTCAACCTTACGAAGGTA+AGG 0.557851 1_3:+54349490 None:intergenic
TGCTCGTGGGAGATGTCCCA+TGG 0.560169 1_3:-54348882 Msa0125760:CDS
GTTCTGAGGTGGAAACTCCA+AGG 0.563503 1_3:-54350523 Msa0125760:CDS
TGGAGCACCTTACCTTCGTA+AGG 0.564503 1_3:-54349497 Msa0125760:CDS
GTGTTGATGTTTCTCCTCAA+GGG 0.566405 1_3:+54350764 None:intergenic
CCAAGGGCTTCTGCGAAGAG+AGG 0.567370 1_3:-54350506 Msa0125760:CDS
CATTTCAGCACCAAGAGCAA+TGG 0.570918 1_3:-54347982 Msa0125760:intron
ATGTTCAGCTCCTTCACCAC+TGG 0.578252 1_3:-54349421 Msa0125760:intron
ATTAACTTATATTATTGAGG+GGG 0.578604 1_3:-54350860 Msa0125760:five_prime_UTR
TAATTAATTACCAGTGGTGA+AGG 0.579528 1_3:+54349411 None:intergenic
AATATGGCCACTATGGGGCA+TGG 0.587296 1_3:-54350620 Msa0125760:CDS
CTTAGCAGGGTCCTTAAGGA+GGG 0.587502 1_3:+54350403 None:intergenic
GTTCAATCCATGCCCCATAG+TGG 0.588585 1_3:+54350613 None:intergenic
ATCGAAGTCCCGTACCAAAG+TGG 0.594032 1_3:-54347904 Msa0125760:three_prime_UTR
AAGCGTCTGCGCATAATGAA+AGG 0.596352 1_3:-54348659 Msa0125760:CDS
GGATGGCGACTGGATGCTCG+TGG 0.596688 1_3:-54348896 Msa0125760:CDS
ATGAAGATAAGGATGGCGAC+TGG 0.598557 1_3:-54348906 Msa0125760:CDS
GCTATAGGAACTTACTCCCA+TGG 0.598945 1_3:+54348865 None:intergenic
AATTAACTTATATTATTGAG+GGG 0.603113 1_3:-54350861 Msa0125760:five_prime_UTR
TTCTGAGGTGGAAACTCCAA+GGG 0.603496 1_3:-54350522 Msa0125760:CDS
GGGAAAACTTACTTAGCTGG+TGG 0.604797 1_3:+54350380 None:intergenic
CGGCGGTGGAGGTGGTTCTG+AGG 0.606000 1_3:-54350537 Msa0125760:CDS
TTCCTGTATGACCTCACTGG+TGG 0.606534 1_3:+54349613 None:intergenic
ATAGGTAACTATGGGGCCCA+AGG 0.608548 1_3:-54348997 Msa0125760:intron
GTACGGGACTTCGATATAGG+AGG 0.608615 1_3:+54347912 None:intergenic
GAAGCTTAATCTTCAAACCA+AGG 0.609730 1_3:-54350462 Msa0125760:CDS
CTTTGTCTTGGTCTGCCTGG+TGG 0.610253 1_3:-54350575 Msa0125760:CDS
AAGGTGCATTATAATACAAG+AGG 0.617418 1_3:-54350795 Msa0125760:five_prime_UTR
CTATAGGAACTTACTCCCAT+GGG 0.622755 1_3:+54348866 None:intergenic
GGGGCATGGATTGAACCTGA+AGG 0.627746 1_3:-54350606 Msa0125760:CDS
CCAACCTATGAAGATAAGGA+TGG 0.646142 1_3:-54348913 Msa0125760:CDS
GCTCGTGGGAGATGTCCCAT+GGG 0.650152 1_3:-54348881 Msa0125760:CDS
AAACTACAATAGTATATAGG+TGG 0.655587 1_3:+54347739 None:intergenic
GTCATACAGGAAGAACATGA+TGG 0.666998 1_3:-54349602 Msa0125760:CDS
GATGGCGACTGGATGCTCGT+GGG 0.667120 1_3:-54348895 Msa0125760:CDS
AAAAGAATATGGCCACTATG+GGG 0.689548 1_3:-54350625 Msa0125760:exon
TAGTTGGTTGGCCACCAGTG+AGG 0.691007 1_3:-54349624 Msa0125760:CDS
ACACAACAAACATGACAGCA+TGG 0.695455 1_3:+54347682 None:intergenic
ATAATAATAATAATTAAACA+AGG 0.699431 1_3:+54347715 None:intergenic
ATTTCTATATAGCCAAACCA+AGG 0.701377 1_3:-54350814 Msa0125760:five_prime_UTR
AAGAGGACAGTACACCCTTG+AGG 0.704580 1_3:-54350778 Msa0125760:five_prime_UTR
ACAAAAGGTTAACAATACTG+AGG 0.707970 1_3:-54349578 Msa0125760:CDS
TTGTCAAGTCAACCTTACGA+AGG 0.716471 1_3:+54349485 None:intergenic
ATATATTATAGGTAACTATG+GGG 0.717921 1_3:-54349004 Msa0125760:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTAATATTATTATA+AGG - chr1_3:54349380-54349399 Msa0125760:intron 0.0%
!! TATTAATTTATATATATTAT+AGG - chr1_3:54349523-54349542 Msa0125760:CDS 0.0%
!!! TTTTTTTTTTATAACTTTCT+TGG - chr1_3:54348212-54348231 Msa0125760:intron 10.0%
!!! ATTATTAATTTGAAGAAAAA+TGG + chr1_3:54348740-54348759 None:intergenic 10.0%
!! TTAAAAAATTGCATAAAATT+TGG + chr1_3:54348816-54348835 None:intergenic 10.0%
!! TCAATTAACTTATATTATTG+AGG - chr1_3:54347675-54347694 Msa0125760:three_prime_UTR 15.0%
!! CAATTAACTTATATTATTGA+GGG - chr1_3:54347676-54347695 Msa0125760:three_prime_UTR 15.0%
!! AATTAACTTATATTATTGAG+GGG - chr1_3:54347677-54347696 Msa0125760:three_prime_UTR 15.0%
!! AATATGAAAAAACGATATAA+GGG + chr1_3:54348510-54348529 None:intergenic 15.0%
!! AAAGAATAATTGAGATTTAT+AGG - chr1_3:54348848-54348867 Msa0125760:intron 15.0%
!! AAGAATAATTGAGATTTATA+GGG - chr1_3:54348849-54348868 Msa0125760:intron 15.0%
!! TAGATTAATTATTATCATCT+TGG - chr1_3:54349453-54349472 Msa0125760:CDS 15.0%
!! AGATTAATTATTATCATCTT+GGG - chr1_3:54349454-54349473 Msa0125760:CDS 15.0%
!! ATATATATTATAGGTAACTA+TGG - chr1_3:54349532-54349551 Msa0125760:CDS 15.0%
!! TATATATTATAGGTAACTAT+GGG - chr1_3:54349533-54349552 Msa0125760:CDS 15.0%
!!! TGGTTATTTAAATTTTTTGA+AGG - chr1_3:54349838-54349857 Msa0125760:intron 15.0%
!!! TTCAATGATTTATTTTTTGT+TGG + chr1_3:54350164-54350183 None:intergenic 15.0%
!! ATGATAATTTAGATATTTGA+AGG + chr1_3:54350306-54350325 None:intergenic 15.0%
!! AAATCATATTTACATTATGT+TGG + chr1_3:54350356-54350375 None:intergenic 15.0%
!! TGTAAATATGATTTAATAGT+TGG - chr1_3:54350362-54350381 Msa0125760:intron 15.0%
!! ATTAACTTATATTATTGAGG+GGG - chr1_3:54347678-54347697 Msa0125760:three_prime_UTR 20.0%
!!! TTGTTTAGTAAATGTTGTTT+TGG + chr1_3:54348269-54348288 None:intergenic 20.0%
!! AAAAAATAGAGAGAGAAAAA+AGG + chr1_3:54348388-54348407 None:intergenic 20.0%
!!! TTTCTTTTCTATCTTCTTAT+TGG + chr1_3:54348416-54348435 None:intergenic 20.0%
!! AATAAGAAGATAGAAAAGAA+AGG - chr1_3:54348415-54348434 Msa0125760:intron 20.0%
!! TATACTATTTGTATATGAGT+TGG - chr1_3:54348479-54348498 Msa0125760:intron 20.0%
!! ATATGAAAAAACGATATAAG+GGG + chr1_3:54348509-54348528 None:intergenic 20.0%
!! CAATATGAAAAAACGATATA+AGG + chr1_3:54348511-54348530 None:intergenic 20.0%
!! AATTTACTAAACAAGCATTA+TGG - chr1_3:54348552-54348571 Msa0125760:intron 20.0%
!!! TTTTTTTCTATCTTCCATTT+GGG + chr1_3:54348587-54348606 None:intergenic 20.0%
!!! TTTTTTTTCTATCTTCCATT+TGG + chr1_3:54348588-54348607 None:intergenic 20.0%
!! AATATTACAAATACAAGCAT+AGG + chr1_3:54349171-54349190 None:intergenic 20.0%
!! ATAAACTATATCAAATAGCT+AGG + chr1_3:54349307-54349326 None:intergenic 20.0%
!! ATATATTATAGGTAACTATG+GGG - chr1_3:54349534-54349553 Msa0125760:CDS 20.0%
!! AGTACATATATATGTTTGTT+GGG + chr1_3:54349771-54349790 None:intergenic 20.0%
!! AAGTACATATATATGTTTGT+TGG + chr1_3:54349772-54349791 None:intergenic 20.0%
!! AACAAACATATATATGTACT+TGG - chr1_3:54349771-54349790 Msa0125760:intron 20.0%
!! AATAACCAATGTTAATTTCA+TGG + chr1_3:54349826-54349845 None:intergenic 20.0%
!!! AATATTTTGTACTTAACTCA+TGG - chr1_3:54349954-54349973 Msa0125760:intron 20.0%
!!! TAATTCTTTTTTTTGAGCAA+TGG - chr1_3:54349996-54350015 Msa0125760:intron 20.0%
!!! TTTATTTTTTGTTGGTCTTA+AGG + chr1_3:54350156-54350175 None:intergenic 20.0%
!! ATTGAATTGAAAATCTTAGA+TGG - chr1_3:54350178-54350197 Msa0125760:intron 20.0%
!!! CTTCATATATCTTTTCTTAT+AGG + chr1_3:54350661-54350680 None:intergenic 20.0%
!!! TTTTCAGTTTTTTCATAGAG+AGG - chr1_3:54347800-54347819 Msa0125760:three_prime_UTR 25.0%
!!! TTTCAGTTTTTTCATAGAGA+GGG - chr1_3:54347801-54347820 Msa0125760:three_prime_UTR 25.0%
! TCAAAATTACAAGCATTTGT+GGG + chr1_3:54348693-54348712 None:intergenic 25.0%
! ATCAAAATTACAAGCATTTG+TGG + chr1_3:54348694-54348713 None:intergenic 25.0%
!!! TTATGCAATTTTTTAAGCCT+TGG - chr1_3:54348820-54348839 Msa0125760:intron 25.0%
! TAGTACTAATTAATTACCAG+TGG + chr1_3:54349136-54349155 None:intergenic 25.0%
!!! ACTTTTTTTTGTTGTGATGA+TGG + chr1_3:54349359-54349378 None:intergenic 25.0%
!!! TATAAGGTTTTAGATCTTGA+AGG - chr1_3:54349396-54349415 Msa0125760:intron 25.0%
!! GTACATATATATGTTTGTTG+GGG + chr1_3:54349770-54349789 None:intergenic 25.0%
!!! ATTTTTTGAAGGATGTTTGT+TGG - chr1_3:54349849-54349868 Msa0125760:intron 25.0%
! GGCTATTTATTCTAACTTAT+TGG - chr1_3:54350516-54350535 Msa0125760:CDS 25.0%
! AAACTACAATAGTATATAGG+TGG + chr1_3:54350802-54350821 None:intergenic 25.0%
ATTGAAGAACTTTGTGTTTG+TGG + chr1_3:54347660-54347679 None:intergenic 30.0%
ATTTCTATATAGCCAAACCA+AGG - chr1_3:54347724-54347743 Msa0125760:three_prime_UTR 30.0%
TATTATAATGCACCTTGGTT+TGG + chr1_3:54347739-54347758 None:intergenic 30.0%
TCTTGTATTATAATGCACCT+TGG + chr1_3:54347744-54347763 None:intergenic 30.0%
AAGGTGCATTATAATACAAG+AGG - chr1_3:54347743-54347762 Msa0125760:three_prime_UTR 30.0%
AATCTTCTTCTTCTAAGCTT+TGG - chr1_3:54347869-54347888 Msa0125760:three_prime_UTR 30.0%
AAAAAGAATATGGCCACTAT+GGG - chr1_3:54347912-54347931 Msa0125760:three_prime_UTR 30.0%
AGCATGCAAGTACTTTAATT+GGG + chr1_3:54348181-54348200 None:intergenic 30.0%
AAGCATGCAAGTACTTTAAT+TGG + chr1_3:54348182-54348201 None:intergenic 30.0%
ATATTTGTACAACTGTTCTG+TGG - chr1_3:54348313-54348332 Msa0125760:intron 30.0%
! CAATTATTCTTTTCTCTCCA+AGG + chr1_3:54348840-54348859 None:intergenic 30.0%
ACAAAAGGTTAACAATACTG+AGG - chr1_3:54348960-54348979 Msa0125760:CDS 30.0%
TAATTAATTACCAGTGGTGA+AGG + chr1_3:54349130-54349149 None:intergenic 30.0%
CTATATCAAATAGCTAGGTA+TGG + chr1_3:54349302-54349321 None:intergenic 30.0%
TGATTGTAGTATATAGTAGC+TGG + chr1_3:54349716-54349735 None:intergenic 30.0%
TTCATGGATGTGTAAATATG+TGG + chr1_3:54349810-54349829 None:intergenic 30.0%
CACATCCATGAAATTAACAT+TGG - chr1_3:54349818-54349837 Msa0125760:intron 30.0%
! TGGTCTTAAGGATTAAGTTT+AGG + chr1_3:54350144-54350163 None:intergenic 30.0%
ATTTAGACCAAAATCAGCTT+CGG + chr1_3:54350595-54350614 None:intergenic 30.0%
GGCAAACTACAATAGTATAT+AGG + chr1_3:54350805-54350824 None:intergenic 30.0%
GAAGCTAGCCAAAAAGAATA+TGG - chr1_3:54347902-54347921 Msa0125760:three_prime_UTR 35.0%
! CATAGTGGCCATATTCTTTT+TGG + chr1_3:54347913-54347932 None:intergenic 35.0%
CAAAAAGAATATGGCCACTA+TGG - chr1_3:54347911-54347930 Msa0125760:three_prime_UTR 35.0%
AAAAGAATATGGCCACTATG+GGG - chr1_3:54347913-54347932 Msa0125760:three_prime_UTR 35.0%
GAAGCTTAATCTTCAAACCA+AGG - chr1_3:54348076-54348095 Msa0125760:intron 35.0%
ATTGGGAAAACTTACTTAGC+TGG + chr1_3:54348164-54348183 None:intergenic 35.0%
ATAGAGAGAGAAAAAAGGAG+AGG + chr1_3:54348383-54348402 None:intergenic 35.0%
TATGGATTCTCAAACCCAAA+TGG - chr1_3:54348570-54348589 Msa0125760:intron 35.0%
! TGCATTTGTTAAGGTTTCCA+TGG - chr1_3:54349017-54349036 Msa0125760:intron 35.0%
TTTGTTAAGGTTTCCATGGA+TGG - chr1_3:54349021-54349040 Msa0125760:intron 35.0%
GGCATCAGATAAATCTTTGT+AGG + chr1_3:54349088-54349107 None:intergenic 35.0%
TAATCTTTCAGCATTCTGTC+TGG + chr1_3:54349226-54349245 None:intergenic 35.0%
AATAGCTAGGTATGGACTTT+AGG + chr1_3:54349294-54349313 None:intergenic 35.0%
TTTAGATCTTGAAGGATGCA+TGG - chr1_3:54349404-54349423 Msa0125760:intron 35.0%
CATGAAGTCTATCATTCCTT+GGG + chr1_3:54349560-54349579 None:intergenic 35.0%
TCATGAAGTCTATCATTCCT+TGG + chr1_3:54349561-54349580 None:intergenic 35.0%
TAGTATATAGTAGCTGGAGA+TGG + chr1_3:54349710-54349729 None:intergenic 35.0%
TGGATGTGTAAATATGTGGT+TGG + chr1_3:54349806-54349825 None:intergenic 35.0%
ATGAAAGGATCAGAAGCTAT+TGG - chr1_3:54349894-54349913 Msa0125760:intron 35.0%
TGAAAGGATCAGAAGCTATT+GGG - chr1_3:54349895-54349914 Msa0125760:intron 35.0%
! TAACTCATGGAAGTATGCTT+TGG - chr1_3:54349967-54349986 Msa0125760:intron 35.0%
TGCATCAAGTATAATGCAAG+TGG + chr1_3:54350092-54350111 None:intergenic 35.0%
! CTTTTCTTATAGGTCCACTT+TGG + chr1_3:54350651-54350670 None:intergenic 35.0%
AAACTAACACTCCAATCAAC+AGG + chr1_3:54350689-54350708 None:intergenic 35.0%
! TGTTGATTGGAGTGTTAGTT+TGG - chr1_3:54350688-54350707 Msa0125760:five_prime_UTR 35.0%
! GTGTTGATGTTTCTCCTCAA+GGG + chr1_3:54347777-54347796 None:intergenic 40.0%
! TGTGTTGATGTTTCTCCTCA+AGG + chr1_3:54347778-54347797 None:intergenic 40.0%
CTTCTCATTCAGATCTTCCT+TGG + chr1_3:54348096-54348115 None:intergenic 40.0%
!! GAATGAGAAGAGTGCTTCAA+AGG - chr1_3:54348106-54348125 Msa0125760:intron 40.0%
TATTTCAGGGCTCAAGTAGT+TGG - chr1_3:54348898-54348917 Msa0125760:CDS 40.0%
GTCATACAGGAAGAACATGA+TGG - chr1_3:54348936-54348955 Msa0125760:CDS 40.0%
GAAGAACATGATGGCACAAA+AGG - chr1_3:54348945-54348964 Msa0125760:CDS 40.0%
! TACTGCTGCTGCATTTGTTA+AGG - chr1_3:54349008-54349027 Msa0125760:intron 40.0%
AAGTCAACCTTACGAAGGTA+AGG + chr1_3:54349051-54349070 None:intergenic 40.0%
TTGTCAAGTCAACCTTACGA+AGG + chr1_3:54349056-54349075 None:intergenic 40.0%
! CATTCTGTCTGGTTTAGATG+GGG + chr1_3:54349215-54349234 None:intergenic 40.0%
! GCATTCTGTCTGGTTTAGAT+GGG + chr1_3:54349216-54349235 None:intergenic 40.0%
! AGCATTCTGTCTGGTTTAGA+TGG + chr1_3:54349217-54349236 None:intergenic 40.0%
! CATGAATGAGAGCAAGTTGA+TGG - chr1_3:54349576-54349595 Msa0125760:CDS 40.0%
TGTGCCAACCTATGAAGATA+AGG - chr1_3:54349621-54349640 Msa0125760:CDS 40.0%
CCATCCTTATCTTCATAGGT+TGG + chr1_3:54349628-54349647 None:intergenic 40.0%
CCAACCTATGAAGATAAGGA+TGG - chr1_3:54349625-54349644 Msa0125760:CDS 40.0%
CTATAGGAACTTACTCCCAT+GGG + chr1_3:54349675-54349694 None:intergenic 40.0%
TATATAGTAGCTGGAGATGG+AGG + chr1_3:54349707-54349726 None:intergenic 40.0%
ACAAAATGACACGCACACAA+GGG + chr1_3:54350496-54350515 None:intergenic 40.0%
TTGCATTTCTCCATTGCTCT+TGG + chr1_3:54350569-54350588 None:intergenic 40.0%
!! GCAAAAACCGAAGCTGATTT+TGG - chr1_3:54350585-54350604 Msa0125760:CDS 40.0%
TTATAGGTCCACTTTGGTAC+GGG + chr1_3:54350645-54350664 None:intergenic 40.0%
CTTATAGGTCCACTTTGGTA+CGG + chr1_3:54350646-54350665 None:intergenic 40.0%
! ATGAAGCACTACCTGTTGAT+TGG - chr1_3:54350675-54350694 Msa0125760:five_prime_UTR 40.0%
ACACAACAAACATGACAGCA+TGG + chr1_3:54350859-54350878 None:intergenic 40.0%
! AAGGAGACTGAGCTTTGTCT+TGG - chr1_3:54347951-54347970 Msa0125760:exon 45.0%
!! TTCTGAGGTGGAAACTCCAA+GGG - chr1_3:54348016-54348035 Msa0125760:intron 45.0%
AAAGGAGAAGACCCTCCTTA+AGG - chr1_3:54348124-54348143 Msa0125760:intron 45.0%
GGGAAAACTTACTTAGCTGG+TGG + chr1_3:54348161-54348180 None:intergenic 45.0%
TTCTTCCTGTATGACCTCAC+TGG + chr1_3:54348931-54348950 None:intergenic 45.0%
!!! GGAGCTGAACATTTTGGCTA+AGG + chr1_3:54349109-54349128 None:intergenic 45.0%
! GGTGAAGGAGCTGAACATTT+TGG + chr1_3:54349115-54349134 None:intergenic 45.0%
GTCGCCATCCTTATCTTCAT+AGG + chr1_3:54349632-54349651 None:intergenic 45.0%
ATGAAGATAAGGATGGCGAC+TGG - chr1_3:54349632-54349651 Msa0125760:CDS 45.0%
GCTATAGGAACTTACTCCCA+TGG + chr1_3:54349676-54349695 None:intergenic 45.0%
ATGGAGGTTGGATGAGCTAT+AGG + chr1_3:54349691-54349710 None:intergenic 45.0%
AAGCGTCTGCGCATAATGAA+AGG - chr1_3:54349879-54349898 Msa0125760:intron 45.0%
AAAATGACACGCACACAAGG+GGG + chr1_3:54350494-54350513 None:intergenic 45.0%
CAAAATGACACGCACACAAG+GGG + chr1_3:54350495-54350514 None:intergenic 45.0%
CACAAAATGACACGCACACA+AGG + chr1_3:54350497-54350516 None:intergenic 45.0%
!!! CTTGTGTGCGTGTCATTTTG+TGG - chr1_3:54350495-54350514 Msa0125760:CDS 45.0%
CATTTCAGCACCAAGAGCAA+TGG - chr1_3:54350556-54350575 Msa0125760:CDS 45.0%
TTGGTACGGGACTTCGATAT+AGG + chr1_3:54350632-54350651 None:intergenic 45.0%
!! TATTATATTAATAATATTTC+AGG - chr1_3:54348884-54348903 Msa0125760:CDS 5.0%
!! ATTATATTAATAATATTTCA+GGG - chr1_3:54348885-54348904 Msa0125760:CDS 5.0%
!! ATTTGTAATATTTATATATA+TGG - chr1_3:54349179-54349198 Msa0125760:intron 5.0%
!! ATAATAATAATAATTAAACA+AGG + chr1_3:54350826-54350845 None:intergenic 5.0%
AAGAGGACAGTACACCCTTG+AGG - chr1_3:54347760-54347779 Msa0125760:three_prime_UTR 50.0%
AATATGGCCACTATGGGGCA+TGG - chr1_3:54347918-54347937 Msa0125760:three_prime_UTR 50.0%
GTTCAATCCATGCCCCATAG+TGG + chr1_3:54347928-54347947 None:intergenic 50.0%
GACAAAGCTCAGTCTCCTTC+AGG + chr1_3:54347950-54347969 None:intergenic 50.0%
!! GTTCTGAGGTGGAAACTCCA+AGG - chr1_3:54348015-54348034 Msa0125760:intron 50.0%
CTTAGCAGGGTCCTTAAGGA+GGG + chr1_3:54348138-54348157 None:intergenic 50.0%
ACTTAGCTGGTGGCTTAGCA+GGG + chr1_3:54348151-54348170 None:intergenic 50.0%
TACTTAGCTGGTGGCTTAGC+AGG + chr1_3:54348152-54348171 None:intergenic 50.0%
TCAGGGCTCAAGTAGTTGGT+TGG - chr1_3:54348902-54348921 Msa0125760:CDS 50.0%
TTCCTGTATGACCTCACTGG+TGG + chr1_3:54348928-54348947 None:intergenic 50.0%
! AAGGTAAGGTGCTCCATCCA+TGG + chr1_3:54349037-54349056 None:intergenic 50.0%
! TGGAGCACCTTACCTTCGTA+AGG - chr1_3:54349041-54349060 Msa0125760:intron 50.0%
ATGTTCAGCTCCTTCACCAC+TGG - chr1_3:54349117-54349136 Msa0125760:intron 50.0%
AGGATGCATGGCGATTGTTG+AGG - chr1_3:54349416-54349435 Msa0125760:intron 50.0%
ATAGGTAACTATGGGGCCCA+AGG - chr1_3:54349541-54349560 Msa0125760:CDS 50.0%
TAGTAGCTGGAGATGGAGGT+TGG + chr1_3:54349703-54349722 None:intergenic 50.0%
GGATCAGAAGCTATTGGGCT+TGG - chr1_3:54349900-54349919 Msa0125760:intron 50.0%
AAATGACACGCACACAAGGG+GGG + chr1_3:54350493-54350512 None:intergenic 50.0%
GTACGGGACTTCGATATAGG+AGG + chr1_3:54350629-54350648 None:intergenic 50.0%
ATCGAAGTCCCGTACCAAAG+TGG - chr1_3:54350634-54350653 Msa0125760:exon 50.0%
! GGGGCATGGATTGAACCTGA+AGG - chr1_3:54347932-54347951 Msa0125760:three_prime_UTR 55.0%
! GAGCTTTGTCTTGGTCTGCC+TGG - chr1_3:54347960-54347979 Msa0125760:CDS 55.0%
CTTTGTCTTGGTCTGCCTGG+TGG - chr1_3:54347963-54347982 Msa0125760:CDS 55.0%
GCTTAGCAGGGTCCTTAAGG+AGG + chr1_3:54348139-54348158 None:intergenic 55.0%
GTGGCTTAGCAGGGTCCTTA+AGG + chr1_3:54348142-54348161 None:intergenic 55.0%
! TAGTTGGTTGGCCACCAGTG+AGG - chr1_3:54348914-54348933 Msa0125760:CDS 55.0%
TGTCTTGGTCTGCCTGGTGG+TGG - chr1_3:54347966-54347985 Msa0125760:CDS 60.0%
CCTCTCTTCGCAGAAGCCCT+TGG + chr1_3:54348035-54348054 None:intergenic 60.0%
CCAAGGGCTTCTGCGAAGAG+AGG - chr1_3:54348032-54348051 Msa0125760:intron 60.0%
GGCCACCAGTGAGGTCATAC+AGG - chr1_3:54348923-54348942 Msa0125760:CDS 60.0%
GATGGCGACTGGATGCTCGT+GGG - chr1_3:54349643-54349662 Msa0125760:intron 60.0%
TGCTCGTGGGAGATGTCCCA+TGG - chr1_3:54349656-54349675 Msa0125760:intron 60.0%
GCTCGTGGGAGATGTCCCAT+GGG - chr1_3:54349657-54349676 Msa0125760:intron 60.0%
GCACACAAGGGGGGACTCAA+GGG + chr1_3:54350484-54350503 None:intergenic 60.0%
!! CTTGGTCTGCCTGGTGGTGG+TGG - chr1_3:54347969-54347988 Msa0125760:CDS 65.0%
!! CGGTGGAGGTGGTTCTGAGG+TGG - chr1_3:54348004-54348023 Msa0125760:intron 65.0%
GGATGGCGACTGGATGCTCG+TGG - chr1_3:54349642-54349661 Msa0125760:intron 65.0%
CGCACACAAGGGGGGACTCA+AGG + chr1_3:54350485-54350504 None:intergenic 65.0%
!! GGTCTGCCTGGTGGTGGTGG+CGG - chr1_3:54347972-54347991 Msa0125760:CDS 70.0%
! CGGCGGTGGAGGTGGTTCTG+AGG - chr1_3:54348001-54348020 Msa0125760:intron 70.0%
!! CTGCCTGGTGGTGGTGGCGG+CGG - chr1_3:54347975-54347994 Msa0125760:CDS 75.0%
!!! CCTGGTGGTGGTGGCGGCGG+CGG - chr1_3:54347978-54347997 Msa0125760:intron 80.0%
! CCGCCGCCGCCACCACCACC+AGG + chr1_3:54347981-54348000 None:intergenic 85.0%
!!! GGTGGTGGTGGCGGCGGCGG+CGG - chr1_3:54347981-54348000 Msa0125760:intron 85.0%
!!! GGTGGTGGCGGCGGCGGCGG+CGG - chr1_3:54347984-54348003 Msa0125760:intron 90.0%
! GGCGGCGGCGGCGGTGGAGG+TGG - chr1_3:54347993-54348012 Msa0125760:intron 90.0%
!! GGTGGCGGCGGCGGCGGCGG+TGG - chr1_3:54347987-54348006 Msa0125760:intron 95.0%
! GGCGGCGGCGGCGGCGGTGG+AGG - chr1_3:54347990-54348009 Msa0125760:intron 95.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 54347632 54350928 54347632 ID=Msa0125760;Name=Msa0125760
chr1_3 mRNA 54347632 54350928 54347632 ID=Msa0125760-mRNA-1;Parent=Msa0125760;Name=Msa0125760-mRNA-1;_AED=0.04;_eAED=0.04;_QI=289|1|1|1|1|1|5|327|238
chr1_3 exon 54347632 54347996 54347632 ID=Msa0125760-mRNA-1:exon:16759;Parent=Msa0125760-mRNA-1
chr1_3 exon 54348639 54348700 54348639 ID=Msa0125760-mRNA-1:exon:16758;Parent=Msa0125760-mRNA-1
chr1_3 exon 54348880 54349015 54348880 ID=Msa0125760-mRNA-1:exon:16757;Parent=Msa0125760-mRNA-1
chr1_3 exon 54349422 54349654 54349422 ID=Msa0125760-mRNA-1:exon:16756;Parent=Msa0125760-mRNA-1
chr1_3 exon 54350392 54350928 54350392 ID=Msa0125760-mRNA-1:exon:16755;Parent=Msa0125760-mRNA-1
chr1_3 five_prime_UTR 54350640 54350928 54350640 ID=Msa0125760-mRNA-1:five_prime_utr;Parent=Msa0125760-mRNA-1
chr1_3 CDS 54350392 54350639 54350392 ID=Msa0125760-mRNA-1:cds;Parent=Msa0125760-mRNA-1
chr1_3 CDS 54349422 54349654 54349422 ID=Msa0125760-mRNA-1:cds;Parent=Msa0125760-mRNA-1
chr1_3 CDS 54348880 54349015 54348880 ID=Msa0125760-mRNA-1:cds;Parent=Msa0125760-mRNA-1
chr1_3 CDS 54348639 54348700 54348639 ID=Msa0125760-mRNA-1:cds;Parent=Msa0125760-mRNA-1
chr1_3 CDS 54347959 54347996 54347959 ID=Msa0125760-mRNA-1:cds;Parent=Msa0125760-mRNA-1
chr1_3 three_prime_UTR 54347632 54347958 54347632 ID=Msa0125760-mRNA-1:three_prime_utr;Parent=Msa0125760-mRNA-1
Gene Sequence

>Msa0125760

ATGGCCACTATGGGGCATGGATTGAACCTGAAGGAGACTGAGCTTTGTCTTGGTCTGCCTGGTGGTGGTGGCGGCGGCGGCGGCGGTGGAGGTGGTTCTGAGGTGGAAACTCCAAGGGCTTCTGCGAAGAGAGGTTTCTCTGAGACTGTTGATCTGAAGCTTAATCTTCAAACCAAGGAAGATCTGAATGAGAAGAGTGCTTCAAAGGAGAAGACCCTCCTTAAGGACCCTGCTAAGCCACCAGCTAAGGCTCAAGTAGTTGGTTGGCCACCAGTGAGGTCATACAGGAAGAACATGATGGCACAAAAGGTTAACAATACTGAGGACACTGAGAAGACAACAAGTAACACTACTGCTGCTGCATTTGTTAAGGTTTCCATGGATGGAGCACCTTACCTTCGTAAGGTTGACTTGACAATGTACAAAACCTACAAAGATTTATCTGATGCCTTAGCCAAAATGTTCAGCTCCTTCACCACTGGTAACTATGGGGCCCAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTTAACAGCTCTGAGTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGAGATGTCCCATGGGAGATGTTTGTTGGATCATGCAAGCGTCTGCGCATAATGAAAGGATCAGAAGCTATTGGGCTTGCACCAAGAGCAATGGAGAAATGCAAAAACCGAAGCTGA
Protein sequence

>Msa0125760

MATMGHGLNLKETELCLGLPGGGGGGGGGGGGSEVETPRASAKRGFSETVDLKLNLQTKEDLNEKSASKEKTLLKDPAKPPAKAQVVGWPPVRSYRKNMMAQKVNNTEDTEKTTSNTTAAAFVKVSMDGAPYLRKVDLTMYKTYKDLSDALAKMFSSFTTGNYGAQGMIDFMNESKLMDLLNSSEYVPTYEDKDGDWMLVGDVPWEMFVGSCKRLRIMKGSEAIGLAPRAMEKCKNRS*