AlfalfaGEDB | Msa0031980

M. sativa cultivar zhongmu-4 / Msa0031980

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363
PFAM: PF12854.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031980	sp\|Q9FRS4\|PPR22_ARATH	50.598	502	242	2	45	546	48	543	4.99e-180	522

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0031950	Msa0031980	0.940514	2.047798e-100	-8.615850e-47
Msa0031980	Msa0043500	0.810617	1.054400e-50	-8.615850e-47
Msa0031980	Msa0043650	0.802078	6.653637e-49	-8.615850e-47
Msa0031980	Msa0079260	0.813453	2.540073e-51	-8.615850e-47
Msa0031980	Msa0126910	0.849628	2.861804e-60	-8.615850e-47
Msa0031980	Msa0172000	0.810741	9.912039e-51	-8.615850e-47
Msa0031980	Msa0324240	0.801060	1.076167e-48	-8.615850e-47
Msa0031980	Msa0514180	0.805348	1.393927e-49	-8.615850e-47
Msa0031980	Msa0795660	0.800002	1.767481e-48	-8.615850e-47
Msa0031980	Msa1130530	0.801339	9.433269e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031980	MtrunA17_Chr1g0190201	93.717	573	33	1	1	570	1	573	0.0	1103
Msa0031980	MtrunA17_Chr1g0190191	29.519	437	306	2	126	561	107	542	6.22e-67	227
Msa0031980	MtrunA17_Chr1g0190191	30.471	361	250	1	108	467	158	518	2.31e-57	201
Msa0031980	MtrunA17_Chr1g0190191	27.564	312	225	1	103	413	223	534	4.02e-36	142
Msa0031980	MtrunA17_Chr1g0190191	28.425	292	174	1	300	556	105	396	7.34e-36	141
Msa0031980	MtrunA17_Chr1g0190191	24.473	237	177	1	328	562	96	332	1.19e-24	108
Msa0031980	MtrunA17_Chr6g0481621	28.539	445	314	1	111	555	124	564	3.05e-60	209
Msa0031980	MtrunA17_Chr6g0481621	25.670	448	294	2	158	570	66	509	1.35e-45	169
Msa0031980	MtrunA17_Chr6g0481621	25.562	356	265	0	98	453	216	571	2.83e-40	154
Msa0031980	MtrunA17_Chr6g0481621	23.971	413	279	1	107	519	190	567	3.65e-38	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031980	AT1G08610.1	50.598	502	242	2	45	546	48	543	0.0	522
Msa0031980	AT1G09900.1	35.378	489	278	2	107	560	102	587	9.52e-101	317
Msa0031980	AT3G04760.1	31.111	450	309	1	112	561	129	577	9.96e-78	257
Msa0031980	AT3G04760.1	29.708	377	230	2	108	449	194	570	2.28e-48	178
Msa0031980	AT3G04760.2	31.209	455	307	2	112	561	94	547	1.05e-75	251
Msa0031980	AT1G63080.2	28.256	453	325	0	109	561	102	554	8.61e-68	231
Msa0031980	AT1G63080.2	27.972	429	274	2	106	499	134	562	1.62e-55	197
Msa0031980	AT1G63080.2	27.230	426	308	2	122	546	45	469	2.99e-47	175
Msa0031980	AT1G63080.2	26.300	327	236	1	103	424	271	597	2.15e-35	141
Msa0031980	AT1G63080.2	28.340	247	173	1	311	553	20	266	1.12e-20	96.7

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Find 117 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAATGTACGGGGAAA+TGG	0.434350	1_1:-58871932	Msa0031980:CDS
AAAGACGGACGTCTGATAGT+AGG	0.515505	1_1:-58871577	Msa0031980:CDS
AAATCAGCAAAAGAAATGTA+CGG	0.534881	1_1:-58871940	Msa0031980:CDS
AAATTATAAAGATACTGCTT+TGG	0.450412	1_1:-58872367	Msa0031980:CDS
AACTGGTTTGCAAACATTGC+GGG	0.321972	1_1:-58872491	Msa0031980:CDS
AAGAGGCTAATGATTTGCAT+CGG	0.619938	1_1:-58871624	Msa0031980:CDS
AAGGCGTAGGTAATCAAATA+AGG	0.380877	1_1:+58872522	None:intergenic
AAGTATTGGAAGATATGGCA+AGG	0.590843	1_1:-58872452	Msa0031980:CDS
AATATGATGGTTATGTCTGG+TGG	0.671900	1_1:-58872759	Msa0031980:CDS
AATCAGCAAAAGAAATGTAC+GGG	0.492677	1_1:-58871939	Msa0031980:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATAATCACGGAAAGGAATT+TGG	+	chr1_1:58871680-58871699	None:intergenic	30.0%
GAATCAAATTTGTGCAAGAA+GGG	+	chr1_1:58871992-58872011	None:intergenic	30.0%
ATTCGAGGTTTAATCAGAAT+TGG	-	chr1_1:58872008-58872027	Msa0031980:CDS	30.0%
ATGAATATGATGGTTATGTC+TGG	-	chr1_1:58872056-58872075	Msa0031980:CDS	30.0%
CAAAAACTAACAGCCAAATT+GGG	+	chr1_1:58872248-58872267	None:intergenic	30.0%
CATATCTTCCAATACTTCAA+GGG	+	chr1_1:58872363-58872382	None:intergenic	30.0%
AGAATTGTACATAGCGATAT+CGG	+	chr1_1:58872405-58872424	None:intergenic	30.0%
GTATTGTAAGTTACAATGTC+TGG	+	chr1_1:58872719-58872738	None:intergenic	30.0%
ACTTATAACATAGCAATCGA+TGG	-	chr1_1:58872833-58872852	Msa0031980:CDS	30.0%
CTTATAACATAGCAATCGAT+GGG	-	chr1_1:58872834-58872853	Msa0031980:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	58871564	58873276	58871564	ID=Msa0031980;Name=Msa0031980
chr1_1	mRNA	58871564	58873276	58871564	ID=Msa0031980-mRNA-1;Parent=Msa0031980;Name=Msa0031980-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|570
chr1_1	exon	58871564	58873276	58871564	ID=Msa0031980-mRNA-1:exon:18016;Parent=Msa0031980-mRNA-1
chr1_1	CDS	58871564	58873276	58871564	ID=Msa0031980-mRNA-1:cds;Parent=Msa0031980-mRNA-1

Gene Sequence

>Msa0031980

ATGAGTTATGCGACGTCTTTCCAAAACAAGGGTTTCAACTTTCACTCTTTCAATCATTGTAACAATAAGGGCACTTGTGTTTTTAGTTTAAGGAAAAAAATCTATACATTGCACCAAATTCCTTTCCGTGATTATAGATGTTGCTCGTCTTGGAAAGGATTGTGGTGTAGAACTGTTTTAAGTGATAGAGTTGATCATTTAGATCGGGGCTTTGAAAGTTATACCGGCAAAAGGGTAGAATCTAGTGAACTAGTGGATTCAGTCACGTCTCTCAACTCTTCAACTTTGTCAGCAGAGGGACAGTTTGTCAGAAACAGTGAATTGACCAACAATAAGATTCTTCAGAGTCTCTGCAGTAGAGGGAAGTTAACGGCTGCAGCAAGATTGGTCGAGGTTATGGCACGTATGAGTCAAATACCTCATTTCCCTTCTTGCACAAATTTGATTCGAGGTTTAATCAGAATTGGTCAGGTAGACAAAGCTTGCAAAATCATGAATATGATGGTTATGTCTGGTGGTGTTCCTGATACGATTACATTCAATGCGATTATTGGAAGCCTTTGTAAAAGAGGCCATTTAAAATCTGCTCTTGAATTTCTTGAAGGTATGAGCTTAAGTGGTTGCATACCAGATGCAAAAACTTATAATACTATAATTCGTTGCATATTTGATAAAGGAGATCCCAATTTGGCTGTTAGTTTTTGGAAGGATCAGTTGAGAAAAGGGTTCCCTCCTTATTTGATTACCTACGCCTTGCTTATTGAACTGGTTTGCAAACATTGCGGGGCTTCTCGAGCCCTTGAAGTATTGGAAGATATGGCAAGGGAAGGTTGTTGTCCCGATATCGCTATGTACAATTCTCTTGTAAGTTTTAGTTCTAAACAAGGAAATTATAAAGATACTGCTTTGGTTATTTCCAATCTTCTATCTCGTGGGATGCAACCAAATGTTGTGACATACAATATCCTCATCCACTCTCTTAGTCTCCATGGGTATTCCGATGTAGTTGATGATATTTTGAAGATTATGAATGAGACTTCCATTTCACCTACACTTGTAACTTACAATATTTTGTTGAATAGTTTATGTAAATCTGGATTTCTAGATCATTCCATAAGCTTATACATCAAAATGGTTTCTGAGAATTGTTCACCAGACATTGTAACTTACAATACCCTTCTTAATGCTTTATGTAAAGAAGGATTTATCGACGAGAGCATTCAATTACTTCACTCTTTGTCTGGTACTAGCTGTTCTCCTGGATTGGTGACTTATAACATAGCAATCGATGGGTTGGCTAGAATGAGATCTATAAAATCAGCAAAAGAAATGTACGGGGAAATGGTGGAAAAAGGAATTGATCCTGATTATATTACCCACAGTACTTTGATATGGGGTCTTTGCCAGGTATATCAATTCGAAGAAGCAGTGGAGATATTGAAAGTGATGCATAGGATAGGACAAAAGATTAAAGGTTATGCTTATAAATGTATTATCCTTGGACTTTGTGAGCAAAAGAAGCTTGATATTGCGATTCAAGCTCTAGACTTGATGGTAAAAGCTCAATGCAAGCCAGATGAGAAAATATATTATACTTTACTTAAGTCTGTTGCTAATGAGGGTATGGTAAAAGAGGCTAATGATTTGCATCGGAGGTTGATTGAGTTGAAGATTCTAAAAGACGGACGTCTGATAGTAGGAGCTCATGCTTAA

Protein sequence

>Msa0031980

MSYATSFQNKGFNFHSFNHCNNKGTCVFSLRKKIYTLHQIPFRDYRCCSSWKGLWCRTVLSDRVDHLDRGFESYTGKRVESSELVDSVTSLNSSTLSAEGQFVRNSELTNNKILQSLCSRGKLTAAARLVEVMARMSQIPHFPSCTNLIRGLIRIGQVDKACKIMNMMVMSGGVPDTITFNAIIGSLCKRGHLKSALEFLEGMSLSGCIPDAKTYNTIIRCIFDKGDPNLAVSFWKDQLRKGFPPYLITYALLIELVCKHCGASRALEVLEDMAREGCCPDIAMYNSLVSFSSKQGNYKDTALVISNLLSRGMQPNVVTYNILIHSLSLHGYSDVVDDILKIMNETSISPTLVTYNILLNSLCKSGFLDHSISLYIKMVSENCSPDIVTYNTLLNALCKEGFIDESIQLLHSLSGTSCSPGLVTYNIAIDGLARMRSIKSAKEMYGEMVEKGIDPDYITHSTLIWGLCQVYQFEEAVEILKVMHRIGQKIKGYAYKCIILGLCEQKKLDIAIQALDLMVKAQCKPDEKIYYTLLKSVANEGMVKEANDLHRRLIELKILKDGRLIVGAHA*