AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0033100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033100 MtrunA17_Chr1g0191681 98.828 256 2 1 1 255 1 256 0.0 518
Msa0033100 MtrunA17_Chr7g0272991 81.154 260 44 2 1 255 1 260 8.29e-145 405
Msa0033100 MtrunA17_Chr2g0310741 58.065 217 75 8 16 226 20 226 1.33e-69 215
Msa0033100 MtrunA17_Chr3g0132191 82.524 103 18 0 83 185 37 139 1.84e-58 185
Msa0033100 MtrunA17_Chr2g0288481 50.000 184 70 7 82 254 66 238 2.27e-53 172
Msa0033100 MtrunA17_Chr2g0318881 59.341 91 36 1 100 189 21 111 8.17e-33 116
Msa0033100 MtrunA17_Chr2g0318921 63.855 83 29 1 100 181 21 103 3.29e-32 114
Msa0033100 MtrunA17_Chr8g0376351 61.333 75 27 1 119 191 1 75 2.48e-27 100
Msa0033100 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 4.26e-12 64.7
Msa0033100 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.08e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033100 AT1G08970.3 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033100 AT1G08970.4 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033100 AT1G08970.2 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033100 AT1G08970.1 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033100 AT1G54830.2 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033100 AT1G54830.3 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033100 AT1G54830.1 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033100 AT1G56170.1 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0033100 AT1G56170.2 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0033100 AT3G48590.1 69.863 146 41 3 83 228 47 189 3.11e-64 201
Msa0033100 AT5G63470.2 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0033100 AT5G63470.1 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0033100 AT5G50480.1 48.276 174 55 7 79 243 35 182 6.37e-44 147
Msa0033100 AT5G27910.1 47.794 136 61 3 78 206 16 148 1.15e-35 125
Msa0033100 AT5G50490.1 39.106 179 90 6 71 244 9 173 3.10e-32 117
Msa0033100 AT5G50470.1 48.148 108 47 2 79 180 44 148 1.20e-28 108
Msa0033100 AT5G38140.1 45.000 100 51 2 80 178 48 144 5.12e-26 101

Find 109 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATATCTTTGATTTCT+TGG 0.152930 1_1:-60348515 Msa0033100:CDS
CTACCAACAACACCCATATT+TGG 0.174332 1_1:+60348985 None:intergenic
TGGAGTTAACCTTGCGTTCT+TGG 0.214555 1_1:-60348603 Msa0033100:CDS
AGATCATTAGATTCTTATTA+TGG 0.246047 1_1:-60348254 Msa0033100:exon
TCATGGCCAAAACCCAAATA+TGG 0.254115 1_1:-60348998 Msa0033100:CDS
AACAACAACTCCAGACCTTT+TGG 0.267438 1_1:-60348783 Msa0033100:CDS
TACAGATGAATAACCAAGTA+TGG 0.276951 1_1:-60348050 Msa0033100:three_prime_UTR
CAACACCCATATTTGGGTTT+TGG 0.289322 1_1:+60348992 None:intergenic
GTCATTGGGTTTGGCTGGTA+TGG 0.344732 1_1:+60348934 None:intergenic
TACCAACAACACCCATATTT+GGG 0.353196 1_1:+60348986 None:intergenic
GTTATATTAGGTGTCTAATA+TGG 0.356708 1_1:-60347874 Msa0033100:three_prime_UTR
TTCTTATTATGGAAGTGTTA+TGG 0.362115 1_1:-60348243 Msa0033100:three_prime_UTR
ATTGTTGTTGCTCCGGTGGT+TGG 0.362576 1_1:+60348284 None:intergenic
TTGTTGTTGCTCCGGTGGTT+GGG 0.366842 1_1:+60348285 None:intergenic
GAAGCATGTGCTGTTATATT+AGG 0.367404 1_1:-60347886 Msa0033100:three_prime_UTR
GGTGACCCAGTCATTGGGTT+TGG 0.375542 1_1:+60348925 None:intergenic
GGGATGGTTGTTCCCTCTGT+TGG 0.377579 1_1:-60348901 Msa0033100:CDS
CAGTAACATTAAGTTCCATT+AGG 0.385447 1_1:+60348084 None:intergenic
GCACAGCAAGCTGGAAATCC+TGG 0.386872 1_1:-60348376 Msa0033100:CDS
GGATGGTTGTTCCCTCTGTT+GGG 0.395560 1_1:-60348900 Msa0033100:CDS
ATCCCAGGTGACCCAGTCAT+TGG 0.407383 1_1:+60348919 None:intergenic
TGAGGCGGCATATAGCAATA+AGG 0.408347 1_1:+60348403 None:intergenic
ACCCAGTCATTGGGTTTGGC+TGG 0.417557 1_1:+60348929 None:intergenic
AACCCAATGACTGGGTCACC+TGG 0.422707 1_1:-60348922 Msa0033100:CDS
TGGAAGTGTTATGGTTAGTA+AGG 0.425704 1_1:-60348234 Msa0033100:three_prime_UTR
GGTGGTTGGGGCCACATTTG+AGG 0.430701 1_1:+60348298 None:intergenic
CACGCCCTTGCAAATACAAC+CGG 0.432250 1_1:+60348640 None:intergenic
TTTACTATGATGTATGGAAA+TGG 0.439756 1_1:+60348145 None:intergenic
CTTGGTTGACATTGTGCCTA+GGG 0.441263 1_1:-60348497 Msa0033100:CDS
AACCCAAATATGGGTGTTGT+TGG 0.445950 1_1:-60348988 Msa0033100:CDS
ACTGATTTCCCCAAAAGGTC+TGG 0.448617 1_1:+60348773 None:intergenic
TCCCAGGTGACCCAGTCATT+GGG 0.450412 1_1:+60348920 None:intergenic
CACAAGATGTTTCTAAGTAC+AGG 0.455147 1_1:-60348002 Msa0033100:three_prime_UTR
AACGCCACAGAAGCACGTTT+CGG 0.455724 1_1:+60350355 None:intergenic
CACATTTGAGGTGCCATGTA+TGG 0.456179 1_1:+60348310 None:intergenic
TCAAATGTGGCCCCAACCAC+CGG 0.464006 1_1:-60348296 Msa0033100:CDS
AGGCGCCGGTTGTATTTGCA+AGG 0.466746 1_1:-60348645 Msa0033100:CDS
TGTTGTTGGTAGTGGACAAA+TGG 0.476420 1_1:-60348974 Msa0033100:CDS
TGTTGTTGCTCCGGTGGTTG+GGG 0.477662 1_1:+60348286 None:intergenic
GTTCCATCAGGTTGACCACC+AGG 0.484332 1_1:+60348868 None:intergenic
CTAATAATGCAAGATCAGAT+TGG 0.485011 1_1:+60347939 None:intergenic
TCGAACACTCTCGTGCAATC+AGG 0.486677 1_1:-60350325 Msa0033100:five_prime_UTR
TCAACCTGATGGAACTCAGC+TGG 0.487041 1_1:-60348860 Msa0033100:CDS
CCAGCAGGCATCGGTCCTCT+TGG 0.488412 1_1:+60348445 None:intergenic
TTGATACTGATTTCCCCAAA+AGG 0.488519 1_1:+60348768 None:intergenic
TTGAGTGGTGATGCTTGCCC+TGG 0.495063 1_1:+60350291 None:intergenic
CCAAGAGGACCGATGCCTGC+TGG 0.496911 1_1:-60348445 Msa0033100:CDS
TGTCCCAGCTGAGTTCCATC+AGG 0.498006 1_1:+60348856 None:intergenic
TCTTGGTTGACATTGTGCCT+AGG 0.499762 1_1:-60348498 Msa0033100:CDS
CCTCAAAATGCACAGCAAGC+TGG 0.503997 1_1:-60348385 Msa0033100:CDS
ATCACACTGAAGAGAACAAA+AGG 0.504661 1_1:-60348579 Msa0033100:CDS
TACCAGCCAAACCCAATGAC+TGG 0.504830 1_1:-60348931 Msa0033100:CDS
GAATCATCATAAACCATACT+TGG 0.506611 1_1:+60348037 None:intergenic
GACTGTGGAGCATACATGTT+GGG 0.509025 1_1:+60348337 None:intergenic
GGCGTGTGAAATGTTCATAT+TGG 0.517805 1_1:-60348623 Msa0033100:CDS
CATTCCGAAACGTGCTTCTG+TGG 0.523815 1_1:-60350359 Msa0033100:five_prime_UTR
CCATCAGCAGGTCCTCCAGC+AGG 0.525310 1_1:+60348430 None:intergenic
TGGTATGGATTAGAACCGTA+TGG 0.527698 1_1:+60348949 None:intergenic
TCTTGATCCTTGCCAAGGGA+AGG 0.527734 1_1:+60348710 None:intergenic
AATATGGGTGTTGTTGGTAG+TGG 0.529665 1_1:-60348982 Msa0033100:CDS
TCATCTTTCAAGTCCTCCCT+AGG 0.530171 1_1:+60348481 None:intergenic
CATGGCCAAAACCCAAATAT+GGG 0.534350 1_1:-60348997 Msa0033100:CDS
ATGGTTAGTAAGGTGAACTT+TGG 0.536359 1_1:-60348224 Msa0033100:three_prime_UTR
GGATTATGCATCATGAGTCC+AGG 0.538672 1_1:+60348358 None:intergenic
CAACCTGATGGAACTCAGCT+GGG 0.543463 1_1:-60348859 Msa0033100:CDS
CAATAAGGAATTCCATCAGC+AGG 0.543484 1_1:+60348418 None:intergenic
GGCGATTGTTGTTGCTCCGG+TGG 0.545817 1_1:+60348280 None:intergenic
AATCTTCTTGATCCTTGCCA+AGG 0.551207 1_1:+60348705 None:intergenic
GATGATATCAGCTGAGGCGC+CGG 0.551962 1_1:-60348659 Msa0033100:CDS
CCTGCTGGAGGACCTGCTGA+TGG 0.553104 1_1:-60348430 Msa0033100:CDS
CAACCTGGTGGTCAACCTGA+TGG 0.553542 1_1:-60348871 Msa0033100:CDS
GAAGGCGATTGTTGTTGCTC+CGG 0.554014 1_1:+60348277 None:intergenic
ATAAGAATCTAATGATCTGA+AGG 0.560767 1_1:+60348259 None:intergenic
GACATTGCAGCAGCAATCAC+AGG 0.562745 1_1:-60348538 Msa0033100:CDS
GAAGATTATGAAGGCTGATG+AGG 0.566353 1_1:-60348689 Msa0033100:CDS
ACCCAATGACTGGGTCACCT+GGG 0.574510 1_1:-60348921 Msa0033100:CDS
GCAGGTCCTCCAGCAGGCAT+CGG 0.575223 1_1:+60348436 None:intergenic
GGTATGGATTAGAACCGTAT+GGG 0.576207 1_1:+60348950 None:intergenic
GGTTGACATTGTGCCTAGGG+AGG 0.582771 1_1:-60348494 Msa0033100:CDS
ATCTTCTTGATCCTTGCCAA+GGG 0.584713 1_1:+60348706 None:intergenic
GTATTGTGATATTGCAGTAG+TGG 0.585313 1_1:-60347964 Msa0033100:three_prime_UTR
TCCACAGTCTCATCCATACA+TGG 0.588194 1_1:-60348323 Msa0033100:CDS
TCCTTGCCAAGGGAAGGCTG+TGG 0.591000 1_1:+60348716 None:intergenic
ACCACAGCCTTCCCTTGGCA+AGG 0.592871 1_1:-60348717 Msa0033100:CDS
GGCGCCGGTTGTATTTGCAA+GGG 0.593275 1_1:-60348644 Msa0033100:CDS
ACAGAGGGAACAACCATCCC+AGG 0.593577 1_1:+60348904 None:intergenic
CGTTTCGGAATGTTCTCGTA+CGG 0.595038 1_1:+60350370 None:intergenic
AAGGATCAAGAAGATTATGA+AGG 0.595150 1_1:-60348698 Msa0033100:CDS
AGTGGACAAATGGCCCCATA+CGG 0.596575 1_1:-60348964 Msa0033100:CDS
AGAATGGATCATCAAGGTCA+TGG 0.604516 1_1:-60349015 Msa0033100:CDS
AATGACTGGGTCACCTGGGA+TGG 0.607597 1_1:-60348917 Msa0033100:CDS
TGAAACAGAATGGATCATCA+AGG 0.611963 1_1:-60349021 Msa0033100:intron
TCTGTTGGGACTATTCAACC+TGG 0.614722 1_1:-60348886 Msa0033100:CDS
CATACATGGCACCTCAAATG+TGG 0.616231 1_1:-60348309 Msa0033100:CDS
AGTTGGTACCTTGTGTTGAG+TGG 0.622959 1_1:+60350276 None:intergenic
ACCAGCCAAACCCAATGACT+GGG 0.624633 1_1:-60348930 Msa0033100:CDS
AGAGGACCGATGCCTGCTGG+AGG 0.625695 1_1:-60348442 Msa0033100:CDS
AAGCATCACCACTCAACACA+AGG 0.628345 1_1:-60350284 Msa0033100:intron
TCAGTATCAAGAAATTGAGA+AGG 0.629465 1_1:-60348755 Msa0033100:CDS
AGTGTGATTCCAAGAACGCA+AGG 0.629484 1_1:+60348594 None:intergenic
TTGAGAACACACTGAACATG+AGG 0.648120 1_1:-60347851 Msa0033100:three_prime_UTR
GGTTGAATAGTCCCAACAGA+GGG 0.660370 1_1:+60348889 None:intergenic
TGTGAAGATGATATCAGCTG+AGG 0.675937 1_1:-60348665 Msa0033100:CDS
GAAGTGCTTGCATTACCAAG+AGG 0.676504 1_1:-60348460 Msa0033100:CDS
GTTGGGACTATTCAACCTGG+TGG 0.686021 1_1:-60348883 Msa0033100:CDS
AGACTGTGGAGCATACATGT+TGG 0.689302 1_1:+60348336 None:intergenic
GTATGGATTAGAACCGTATG+GGG 0.698616 1_1:+60348951 None:intergenic
GCCATGTATGGATGAGACTG+TGG 0.701999 1_1:+60348322 None:intergenic
AGGTTGAATAGTCCCAACAG+AGG 0.742829 1_1:+60348888 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGGATTTTTTTTTTTTTTT+AGG - chr1_1:60348350-60348369 Msa0033100:CDS 10.0%
!!! AGGATTTTTTTTTTTTTTTA+GGG - chr1_1:60348351-60348370 Msa0033100:CDS 10.0%
!!! AAATGTTATATGATATTTTA+CGG - chr1_1:60348576-60348595 Msa0033100:CDS 10.0%
!!! TTTTTTTTTTTTTTTAGGGT+TGG - chr1_1:60348355-60348374 Msa0033100:CDS 15.0%
!! CAGTATATATTATAACTATA+TGG + chr1_1:60348381-60348400 None:intergenic 15.0%
!! TAATATATACTGTAAATTGT+TGG - chr1_1:60348389-60348408 Msa0033100:CDS 15.0%
!! GTAAAATATCATATAACATT+TGG + chr1_1:60348577-60348596 None:intergenic 15.0%
!!! TTTAGGAGAAAAATTAATTT+TGG - chr1_1:60348704-60348723 Msa0033100:CDS 15.0%
!!! TTAGGAGAAAAATTAATTTT+GGG - chr1_1:60348705-60348724 Msa0033100:CDS 15.0%
!! AATCGAATTACATAATGAAA+AGG + chr1_1:60347957-60347976 None:intergenic 20.0%
!!! TTGTTTAGTTTTTCTTGTTA+CGG - chr1_1:60347975-60347994 Msa0033100:three_prime_UTR 20.0%
!! AGCAAATATCTAATATTTCA+TGG + chr1_1:60348057-60348076 None:intergenic 20.0%
!! TGAAATATTAGATATTTGCT+TGG - chr1_1:60348057-60348076 Msa0033100:three_prime_UTR 20.0%
!! AGCAGATTTATTTATGAATA+TGG - chr1_1:60348109-60348128 Msa0033100:three_prime_UTR 20.0%
!!! TATTTCTTTTATTCCATTCA+AGG - chr1_1:60348287-60348306 Msa0033100:CDS 20.0%
!! AGATCATTAGATTCTTATTA+TGG - chr1_1:60349931-60349950 Msa0033100:intron 20.0%
!!! TGCAATAAATTAGTTTTACA+AGG - chr1_1:60349984-60350003 Msa0033100:intron 20.0%
!!! GCAATAAATTAGTTTTACAA+GGG - chr1_1:60349985-60350004 Msa0033100:intron 20.0%
!!! TTAAGTTTTACTATGATGTA+TGG + chr1_1:60350049-60350068 None:intergenic 20.0%
!!! TAATAATTTTGTATGTTGTG+TGG - chr1_1:60350277-60350296 Msa0033100:intron 20.0%
! AATATTAGATATTTGCTTGG+TGG - chr1_1:60348060-60348079 Msa0033100:three_prime_UTR 25.0%
! TTATGAATATGGTGAGAATT+AGG - chr1_1:60348120-60348139 Msa0033100:three_prime_UTR 25.0%
! TATGAATATGGTGAGAATTA+GGG - chr1_1:60348121-60348140 Msa0033100:three_prime_UTR 25.0%
! TTCCAAATACATGATTGATT+TGG - chr1_1:60348259-60348278 Msa0033100:exon 25.0%
! AACACAAAAAAAACCTTGAA+TGG + chr1_1:60348303-60348322 None:intergenic 25.0%
!!! GGTTTTTTTTGTGTTTTTCA+TGG - chr1_1:60348308-60348327 Msa0033100:CDS 25.0%
! ATATACTGTAAATTGTTGGT+TGG - chr1_1:60348393-60348412 Msa0033100:CDS 25.0%
! ATGTTTAATATGTTGCAAAG+AGG - chr1_1:60348500-60348519 Msa0033100:CDS 25.0%
! TTTAATGCCAATAGCTATTT+AGG - chr1_1:60348687-60348706 Msa0033100:CDS 25.0%
!! TTTTTCTCCTAAATAGCTAT+TGG + chr1_1:60348697-60348716 None:intergenic 25.0%
!!! GAGAAAAATTAATTTTGGGT+AGG - chr1_1:60348709-60348728 Msa0033100:CDS 25.0%
! ATAACTTTGTACAAGTGTAT+TGG - chr1_1:60348821-60348840 Msa0033100:CDS 25.0%
!! AATTAAGATTTGTTCGAGTT+TGG - chr1_1:60348848-60348867 Msa0033100:CDS 25.0%
!!! ATGTGCATATTGTTTTGTTT+TGG - chr1_1:60349101-60349120 Msa0033100:intron 25.0%
!! AACTGATATCTTTGATTTCT+TGG - chr1_1:60349670-60349689 Msa0033100:intron 25.0%
! ATAAGAATCTAATGATCTGA+AGG + chr1_1:60349929-60349948 None:intergenic 25.0%
!!! TTCTTATTATGGAAGTGTTA+TGG - chr1_1:60349942-60349961 Msa0033100:intron 25.0%
! TTTACTATGATGTATGGAAA+TGG + chr1_1:60350043-60350062 None:intergenic 25.0%
!!! TTCAATGTCATTTTTCCTAA+TGG - chr1_1:60350086-60350105 Msa0033100:intron 25.0%
! GTTATATTAGGTGTCTAATA+TGG - chr1_1:60350311-60350330 Msa0033100:five_prime_UTR 25.0%
!!! AATCAGGTTATTGATTTTCC+AGG - chr1_1:60347876-60347895 Msa0033100:three_prime_UTR 30.0%
!!! ATCAGGTTATTGATTTTCCA+GGG - chr1_1:60347877-60347896 Msa0033100:three_prime_UTR 30.0%
! CTTGTTATGCTTGATGTATA+TGG - chr1_1:60348187-60348206 Msa0033100:three_prime_UTR 30.0%
CTCCAAATCAATCATGTATT+TGG + chr1_1:60348264-60348283 None:intergenic 30.0%
!! CAAATACATGATTGATTTGG+AGG - chr1_1:60348262-60348281 Msa0033100:exon 30.0%
ACTTCAGTACAAAGTTATGT+CGG + chr1_1:60348967-60348986 None:intergenic 30.0%
AACTTTGTACTGAAGTGTAT+TGG - chr1_1:60348971-60348990 Msa0033100:CDS 30.0%
! TAAAGATTTTGTTCAAGTCC+TGG - chr1_1:60348999-60349018 Msa0033100:CDS 30.0%
ACAATTACACGTGAACAAAA+GGG + chr1_1:60349075-60349094 None:intergenic 30.0%
AACAATTACACGTGAACAAA+AGG + chr1_1:60349076-60349095 None:intergenic 30.0%
TGATTTCACTTGAAACAGAA+TGG - chr1_1:60349154-60349173 Msa0033100:intron 30.0%
TCAGTATCAAGAAATTGAGA+AGG - chr1_1:60349430-60349449 Msa0033100:intron 30.0%
AAGGATCAAGAAGATTATGA+AGG - chr1_1:60349487-60349506 Msa0033100:intron 30.0%
! TGCAATGTCATTCTTTTGAA+GGG + chr1_1:60349636-60349655 None:intergenic 30.0%
CAGTAACATTAAGTTCCATT+AGG + chr1_1:60350104-60350123 None:intergenic 30.0%
TACAGATGAATAACCAAGTA+TGG - chr1_1:60350135-60350154 Msa0033100:intron 30.0%
GAATCATCATAAACCATACT+TGG + chr1_1:60350151-60350170 None:intergenic 30.0%
CTAATAATGCAAGATCAGAT+TGG + chr1_1:60350249-60350268 None:intergenic 30.0%
!! CACTAAATTCTGGGGTTAAT+CGG + chr1_1:60348027-60348046 None:intergenic 35.0%
!! TTTGCTTGGTGGTTTTTATG+CGG - chr1_1:60348071-60348090 Msa0033100:three_prime_UTR 35.0%
!! AAATATACACAAGTGCTGGA+TGG + chr1_1:60348240-60348259 None:intergenic 35.0%
TGGAAAATATACACAAGTGC+TGG + chr1_1:60348244-60348263 None:intergenic 35.0%
! TTTTGTGTTTTTCATGGTGC+TGG - chr1_1:60348314-60348333 Msa0033100:CDS 35.0%
! TTTGTGTTTTTCATGGTGCT+GGG - chr1_1:60348315-60348334 Msa0033100:CDS 35.0%
!! TATAACATTTGGTAGCGTAG+TGG + chr1_1:60348566-60348585 None:intergenic 35.0%
!! AATTAATTTTGGGTAGGCCA+TGG - chr1_1:60348715-60348734 Msa0033100:CDS 35.0%
AAACAATGTTTATCGCGTAG+CGG + chr1_1:60348798-60348817 None:intergenic 35.0%
CAATTACACGTGAACAAAAG+GGG + chr1_1:60349074-60349093 None:intergenic 35.0%
TGAAACAGAATGGATCATCA+AGG - chr1_1:60349164-60349183 Msa0033100:intron 35.0%
TACCAACAACACCCATATTT+GGG + chr1_1:60349202-60349221 None:intergenic 35.0%
!! TTGATACTGATTTCCCCAAA+AGG + chr1_1:60349420-60349439 None:intergenic 35.0%
ATCACACTGAAGAGAACAAA+AGG - chr1_1:60349606-60349625 Msa0033100:intron 35.0%
! CTGCAATGTCATTCTTTTGA+AGG + chr1_1:60349637-60349656 None:intergenic 35.0%
!! TGGAAGTGTTATGGTTAGTA+AGG - chr1_1:60349951-60349970 Msa0033100:intron 35.0%
ATGGTTAGTAAGGTGAACTT+TGG - chr1_1:60349961-60349980 Msa0033100:intron 35.0%
! CACAAGATGTTTCTAAGTAC+AGG - chr1_1:60350183-60350202 Msa0033100:intron 35.0%
GTATTGTGATATTGCAGTAG+TGG - chr1_1:60350221-60350240 Msa0033100:intron 35.0%
! GAAGCATGTGCTGTTATATT+AGG - chr1_1:60350299-60350318 Msa0033100:five_prime_UTR 35.0%
ACCCCAGAATTTAGTGTTTG+TGG - chr1_1:60348031-60348050 Msa0033100:three_prime_UTR 40.0%
GCCACAAACACTAAATTCTG+GGG + chr1_1:60348035-60348054 None:intergenic 40.0%
GGCCACAAACACTAAATTCT+GGG + chr1_1:60348036-60348055 None:intergenic 40.0%
TGGCCACAAACACTAAATTC+TGG + chr1_1:60348037-60348056 None:intergenic 40.0%
!! GTTGGTTGGTAAGACTTGAT+TGG - chr1_1:60348407-60348426 Msa0033100:CDS 40.0%
TGGTATAATTGTCGAGACGA+AGG - chr1_1:60348427-60348446 Msa0033100:CDS 40.0%
AACAATGTTTATCGCGTAGC+GGG + chr1_1:60348797-60348816 None:intergenic 40.0%
! TGTCGGTGTTTATCATGTAG+TGG + chr1_1:60348950-60348969 None:intergenic 40.0%
CTAGAATGCTATAGCATACC+AGG + chr1_1:60349020-60349039 None:intergenic 40.0%
TCAATTCCTGCACTTCACAT+GGG + chr1_1:60349128-60349147 None:intergenic 40.0%
ATCAATTCCTGCACTTCACA+TGG + chr1_1:60349129-60349148 None:intergenic 40.0%
AGAATGGATCATCAAGGTCA+TGG - chr1_1:60349170-60349189 Msa0033100:intron 40.0%
TCATGGCCAAAACCCAAATA+TGG - chr1_1:60349187-60349206 Msa0033100:intron 40.0%
CATGGCCAAAACCCAAATAT+GGG - chr1_1:60349188-60349207 Msa0033100:intron 40.0%
!! CAACACCCATATTTGGGTTT+TGG + chr1_1:60349196-60349215 None:intergenic 40.0%
! AACCCAAATATGGGTGTTGT+TGG - chr1_1:60349197-60349216 Msa0033100:intron 40.0%
CTACCAACAACACCCATATT+TGG + chr1_1:60349203-60349222 None:intergenic 40.0%
!! AATATGGGTGTTGTTGGTAG+TGG - chr1_1:60349203-60349222 Msa0033100:intron 40.0%
!! TGTTGTTGGTAGTGGACAAA+TGG - chr1_1:60349211-60349230 Msa0033100:intron 40.0%
!! GTATGGATTAGAACCGTATG+GGG + chr1_1:60349237-60349256 None:intergenic 40.0%
!! GGTATGGATTAGAACCGTAT+GGG + chr1_1:60349238-60349257 None:intergenic 40.0%
!! TGGTATGGATTAGAACCGTA+TGG + chr1_1:60349239-60349258 None:intergenic 40.0%
! AACAACAACTCCAGACCTTT+TGG - chr1_1:60349402-60349421 Msa0033100:intron 40.0%
! ACAACAACTCCAGACCTTTT+GGG - chr1_1:60349403-60349422 Msa0033100:intron 40.0%
ATCTTCTTGATCCTTGCCAA+GGG + chr1_1:60349482-60349501 None:intergenic 40.0%
AATCTTCTTGATCCTTGCCA+AGG + chr1_1:60349483-60349502 None:intergenic 40.0%
GAAGATTATGAAGGCTGATG+AGG - chr1_1:60349496-60349515 Msa0033100:intron 40.0%
TGTGAAGATGATATCAGCTG+AGG - chr1_1:60349520-60349539 Msa0033100:intron 40.0%
GGCGTGTGAAATGTTCATAT+TGG - chr1_1:60349562-60349581 Msa0033100:intron 40.0%
CAATAAGGAATTCCATCAGC+AGG + chr1_1:60349770-60349789 None:intergenic 40.0%
TTGAGAACACACTGAACATG+AGG - chr1_1:60350334-60350353 Msa0033100:five_prime_UTR 40.0%
CGTTTCGGAATGTTCTCGTA+CGG + chr1_1:60347818-60347837 None:intergenic 45.0%
AAGCATCACCACTCAACACA+AGG - chr1_1:60347901-60347920 Msa0033100:three_prime_UTR 45.0%
! AGTTGGTACCTTGTGTTGAG+TGG + chr1_1:60347912-60347931 None:intergenic 45.0%
AGTACGCGAATTGCAAGAGT+TGG + chr1_1:60347929-60347948 None:intergenic 45.0%
!! TGTTTTTCATGGTGCTGGGA+AGG - chr1_1:60348319-60348338 Msa0033100:CDS 45.0%
!! GTTTTTCATGGTGCTGGGAA+GGG - chr1_1:60348320-60348339 Msa0033100:CDS 45.0%
!! TTTTTCATGGTGCTGGGAAG+GGG - chr1_1:60348321-60348340 Msa0033100:CDS 45.0%
ACAATGTTTATCGCGTAGCG+GGG + chr1_1:60348796-60348815 None:intergenic 45.0%
TCCACAATTGACACCATTGG+CGG + chr1_1:60348900-60348919 None:intergenic 45.0%
CAGTCCACAATTGACACCAT+TGG + chr1_1:60348903-60348922 None:intergenic 45.0%
! ATGGTGTCAATTGTGGACTG+CGG - chr1_1:60348903-60348922 Msa0033100:CDS 45.0%
CAATTCCTGCACTTCACATG+GGG + chr1_1:60349127-60349146 None:intergenic 45.0%
GGTTGAATAGTCCCAACAGA+GGG + chr1_1:60349299-60349318 None:intergenic 45.0%
AGGTTGAATAGTCCCAACAG+AGG + chr1_1:60349300-60349319 None:intergenic 45.0%
TCTGTTGGGACTATTCAACC+TGG - chr1_1:60349299-60349318 Msa0033100:intron 45.0%
! CAACAACTCCAGACCTTTTG+GGG - chr1_1:60349404-60349423 Msa0033100:intron 45.0%
! ACTGATTTCCCCAAAAGGTC+TGG + chr1_1:60349415-60349434 None:intergenic 45.0%
TGGAGTTAACCTTGCGTTCT+TGG - chr1_1:60349582-60349601 Msa0033100:intron 45.0%
AGTGTGATTCCAAGAACGCA+AGG + chr1_1:60349594-60349613 None:intergenic 45.0%
TCTTGGTTGACATTGTGCCT+AGG - chr1_1:60349687-60349706 Msa0033100:intron 45.0%
CTTGGTTGACATTGTGCCTA+GGG - chr1_1:60349688-60349707 Msa0033100:intron 45.0%
! TCATCTTTCAAGTCCTCCCT+AGG + chr1_1:60349707-60349726 None:intergenic 45.0%
! GAAGTGCTTGCATTACCAAG+AGG - chr1_1:60349725-60349744 Msa0033100:intron 45.0%
TGAGGCGGCATATAGCAATA+AGG + chr1_1:60349785-60349804 None:intergenic 45.0%
GGATTATGCATCATGAGTCC+AGG + chr1_1:60349830-60349849 None:intergenic 45.0%
GACTGTGGAGCATACATGTT+GGG + chr1_1:60349851-60349870 None:intergenic 45.0%
AGACTGTGGAGCATACATGT+TGG + chr1_1:60349852-60349871 None:intergenic 45.0%
TCCACAGTCTCATCCATACA+TGG - chr1_1:60349862-60349881 Msa0033100:intron 45.0%
! CACATTTGAGGTGCCATGTA+TGG + chr1_1:60349878-60349897 None:intergenic 45.0%
CATACATGGCACCTCAAATG+TGG - chr1_1:60349876-60349895 Msa0033100:intron 45.0%
CATTCCGAAACGTGCTTCTG+TGG - chr1_1:60347826-60347845 Msa0033100:three_prime_UTR 50.0%
! AACGCCACAGAAGCACGTTT+CGG + chr1_1:60347833-60347852 None:intergenic 50.0%
TCGAACACTCTCGTGCAATC+AGG - chr1_1:60347860-60347879 Msa0033100:three_prime_UTR 50.0%
ATCCGCAAACGACACCAGTA+GGG + chr1_1:60348749-60348768 None:intergenic 50.0%
AATCCGCAAACGACACCAGT+AGG + chr1_1:60348750-60348769 None:intergenic 50.0%
TCAAATCACAGCTCCGCCAA+TGG - chr1_1:60348884-60348903 Msa0033100:CDS 50.0%
! TCCGCCAATGGTGTCAATTG+TGG - chr1_1:60348896-60348915 Msa0033100:CDS 50.0%
AGTGGACAAATGGCCCCATA+CGG - chr1_1:60349221-60349240 Msa0033100:intron 50.0%
GTCATTGGGTTTGGCTGGTA+TGG + chr1_1:60349254-60349273 None:intergenic 50.0%
TACCAGCCAAACCCAATGAC+TGG - chr1_1:60349254-60349273 Msa0033100:intron 50.0%
ACCAGCCAAACCCAATGACT+GGG - chr1_1:60349255-60349274 Msa0033100:intron 50.0%
GGATGGTTGTTCCCTCTGTT+GGG - chr1_1:60349285-60349304 Msa0033100:intron 50.0%
GTTGGGACTATTCAACCTGG+TGG - chr1_1:60349302-60349321 Msa0033100:intron 50.0%
TCAACCTGATGGAACTCAGC+TGG - chr1_1:60349325-60349344 Msa0033100:intron 50.0%
CAACCTGATGGAACTCAGCT+GGG - chr1_1:60349326-60349345 Msa0033100:intron 50.0%
CAAAAACCACAGCCTTCCCT+TGG - chr1_1:60349463-60349482 Msa0033100:intron 50.0%
TCTTGATCCTTGCCAAGGGA+AGG + chr1_1:60349478-60349497 None:intergenic 50.0%
CACGCCCTTGCAAATACAAC+CGG + chr1_1:60349548-60349567 None:intergenic 50.0%
GACATTGCAGCAGCAATCAC+AGG - chr1_1:60349647-60349666 Msa0033100:intron 50.0%
!!! GCTTGCTGTGCATTTTGAGG+CGG + chr1_1:60349800-60349819 None:intergenic 50.0%
!!! CCAGCTTGCTGTGCATTTTG+AGG + chr1_1:60349803-60349822 None:intergenic 50.0%
CCTCAAAATGCACAGCAAGC+TGG - chr1_1:60349800-60349819 Msa0033100:intron 50.0%
GCCATGTATGGATGAGACTG+TGG + chr1_1:60349866-60349885 None:intergenic 50.0%
! TTGTTGTTGCTCCGGTGGTT+GGG + chr1_1:60349903-60349922 None:intergenic 50.0%
! ATTGTTGTTGCTCCGGTGGT+TGG + chr1_1:60349904-60349923 None:intergenic 50.0%
! GAAGGCGATTGTTGTTGCTC+CGG + chr1_1:60349911-60349930 None:intergenic 50.0%
! TTGAGTGGTGATGCTTGCCC+TGG + chr1_1:60347897-60347916 None:intergenic 55.0%
! TTTTGTCCCTCGTAGGTCGC+AGG + chr1_1:60348533-60348552 None:intergenic 55.0%
GACCTACGAGGGACAAAACG+CGG - chr1_1:60348535-60348554 Msa0033100:CDS 55.0%
ACCTACGAGGGACAAAACGC+GGG - chr1_1:60348536-60348555 Msa0033100:CDS 55.0%
TCCGCAAACGACACCAGTAG+GGG + chr1_1:60348748-60348767 None:intergenic 55.0%
!! CTGGTGTCGTTTGCGGATTG+CGG - chr1_1:60348751-60348770 Msa0033100:CDS 55.0%
TTTGGCCCCATGTGAAGTGC+AGG - chr1_1:60349119-60349138 Msa0033100:intron 55.0%
ACCCAGTCATTGGGTTTGGC+TGG + chr1_1:60349259-60349278 None:intergenic 55.0%
GGTGACCCAGTCATTGGGTT+TGG + chr1_1:60349263-60349282 None:intergenic 55.0%
AACCCAATGACTGGGTCACC+TGG - chr1_1:60349263-60349282 Msa0033100:intron 55.0%
ACCCAATGACTGGGTCACCT+GGG - chr1_1:60349264-60349283 Msa0033100:intron 55.0%
TCCCAGGTGACCCAGTCATT+GGG + chr1_1:60349268-60349287 None:intergenic 55.0%
ATCCCAGGTGACCCAGTCAT+TGG + chr1_1:60349269-60349288 None:intergenic 55.0%
AATGACTGGGTCACCTGGGA+TGG - chr1_1:60349268-60349287 Msa0033100:intron 55.0%
ACAGAGGGAACAACCATCCC+AGG + chr1_1:60349284-60349303 None:intergenic 55.0%
GGGATGGTTGTTCCCTCTGT+TGG - chr1_1:60349284-60349303 Msa0033100:intron 55.0%
CAACCTGGTGGTCAACCTGA+TGG - chr1_1:60349314-60349333 Msa0033100:intron 55.0%
!! GTTCCATCAGGTTGACCACC+AGG + chr1_1:60349320-60349339 None:intergenic 55.0%
TGTCCCAGCTGAGTTCCATC+AGG + chr1_1:60349332-60349351 None:intergenic 55.0%
GATGATATCAGCTGAGGCGC+CGG - chr1_1:60349526-60349545 Msa0033100:intron 55.0%
!! AGGCGCCGGTTGTATTTGCA+AGG - chr1_1:60349540-60349559 Msa0033100:intron 55.0%
!! GGCGCCGGTTGTATTTGCAA+GGG - chr1_1:60349541-60349560 Msa0033100:intron 55.0%
GGTTGACATTGTGCCTAGGG+AGG - chr1_1:60349691-60349710 Msa0033100:intron 55.0%
GCACAGCAAGCTGGAAATCC+TGG - chr1_1:60349809-60349828 Msa0033100:intron 55.0%
TCAAATGTGGCCCCAACCAC+CGG - chr1_1:60349889-60349908 Msa0033100:intron 55.0%
TGTTGTTGCTCCGGTGGTTG+GGG + chr1_1:60349902-60349921 None:intergenic 55.0%
TGCTGGGAAGGGGTGAAGCT+AGG - chr1_1:60348331-60348350 Msa0033100:CDS 60.0%
ACTCGATCCTGCGACCTACG+AGG - chr1_1:60348523-60348542 Msa0033100:CDS 60.0%
CTCGATCCTGCGACCTACGA+GGG - chr1_1:60348524-60348543 Msa0033100:CDS 60.0%
! ACCCGCGTTTTGTCCCTCGT+AGG + chr1_1:60348540-60348559 None:intergenic 60.0%
CCGCAAACGACACCAGTAGG+GGG + chr1_1:60348747-60348766 None:intergenic 60.0%
!! CCCCCTACTGGTGTCGTTTG+CGG - chr1_1:60348744-60348763 Msa0033100:CDS 60.0%
ACCACAGCCTTCCCTTGGCA+AGG - chr1_1:60349468-60349487 Msa0033100:intron 60.0%
TCCTTGCCAAGGGAAGGCTG+TGG + chr1_1:60349472-60349491 None:intergenic 60.0%
GGTGGTTGGGGCCACATTTG+AGG + chr1_1:60349890-60349909 None:intergenic 60.0%
! GGCGATTGTTGTTGCTCCGG+TGG + chr1_1:60349908-60349927 None:intergenic 60.0%
CCAGTAGGGGGAGCTAGCCA+TGG + chr1_1:60348735-60348754 None:intergenic 65.0%
! CCATGGCTAGCTCCCCCTAC+TGG - chr1_1:60348732-60348751 Msa0033100:CDS 65.0%
!! CCAGCAGGCATCGGTCCTCT+TGG + chr1_1:60349743-60349762 None:intergenic 65.0%
CCAAGAGGACCGATGCCTGC+TGG - chr1_1:60349740-60349759 Msa0033100:intron 65.0%
AGAGGACCGATGCCTGCTGG+AGG - chr1_1:60349743-60349762 Msa0033100:intron 65.0%
GCAGGTCCTCCAGCAGGCAT+CGG + chr1_1:60349752-60349771 None:intergenic 65.0%
CCATCAGCAGGTCCTCCAGC+AGG + chr1_1:60349758-60349777 None:intergenic 65.0%
CCTGCTGGAGGACCTGCTGA+TGG - chr1_1:60349755-60349774 Msa0033100:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60347811 60350396 60347811 ID=Msa0033100;Name=Msa0033100
chr1_1 mRNA 60347811 60350396 60347811 ID=Msa0033100-mRNA-1;Parent=Msa0033100;Name=Msa0033100-mRNA-1;_AED=0.01;_eAED=0.01;_QI=112|0|0.5|1|0|0.5|2|456|255
chr1_1 exon 60347811 60349034 60347811 ID=Msa0033100-mRNA-1:exon:18893;Parent=Msa0033100-mRNA-1
chr1_1 exon 60350285 60350396 60350285 ID=Msa0033100-mRNA-1:exon:18892;Parent=Msa0033100-mRNA-1
chr1_1 five_prime_UTR 60350285 60350396 60350285 ID=Msa0033100-mRNA-1:five_prime_utr;Parent=Msa0033100-mRNA-1
chr1_1 CDS 60348267 60349034 60348267 ID=Msa0033100-mRNA-1:cds;Parent=Msa0033100-mRNA-1
chr1_1 three_prime_UTR 60347811 60348266 60347811 ID=Msa0033100-mRNA-1:three_prime_utr;Parent=Msa0033100-mRNA-1
Gene Sequence

>Msa0033100

ATGGATCATCAAGGTCATGGCCAAAACCCAAATATGGGTGTTGTTGGTAGTGGACAAATGGCCCCATACGGTTCTAATCCATACCAGCCAAACCCAATGACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGACTATTCAACCTGGTGGTCAACCTGATGGAACTCAGCTGGGACAACATCAGCTTGCTTATCAGCACATTCATCAGCAGCAACAGCAGCAGCAACAACAACAACTCCAGACCTTTTGGGGAAATCAGTATCAAGAAATTGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCTGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGTGAAATGTTCATATTGGAGTTAACCTTGCGTTCTTGGAATCACACTGAAGAGAACAAAAGGCGAACCCTTCAAAAGAATGACATTGCAGCAGCAATCACAGGAACTGATATCTTTGATTTCTTGGTTGACATTGTGCCTAGGGAGGACTTGAAAGATGAAGTGCTTGCATTACCAAGAGGACCGATGCCTGCTGGAGGACCTGCTGATGGAATTCCTTATTGCTATATGCCGCCTCAAAATGCACAGCAAGCTGGAAATCCTGGACTCATGATGCATAATCCCAACATGTATGCTCCACAGTCTCATCCATACATGGCACCTCAAATGTGGCCCCAACCACCGGAGCAACAACAATCGCCTTCAGATCATTAG
Protein sequence

>Msa0033100

MDHQGHGQNPNMGVVGSGQMAPYGSNPYQPNPMTGSPGMVVPSVGTIQPGGQPDGTQLGQHQLAYQHIHQQQQQQQQQQLQTFWGNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVKMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITGTDIFDFLVDIVPREDLKDEVLALPRGPMPAGGPADGIPYCYMPPQNAQQAGNPGLMMHNPNMYAPQSHPYMAPQMWPQPPEQQQSPSDH*