AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0091400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0091400 MtrunA17_Chr1g0191681 98.828 256 2 1 1 255 1 256 0.0 518
Msa0091400 MtrunA17_Chr7g0272991 81.154 260 44 2 1 255 1 260 8.29e-145 405
Msa0091400 MtrunA17_Chr2g0310741 58.065 217 75 8 16 226 20 226 1.33e-69 215
Msa0091400 MtrunA17_Chr3g0132191 82.524 103 18 0 83 185 37 139 1.84e-58 185
Msa0091400 MtrunA17_Chr2g0288481 50.000 184 70 7 82 254 66 238 2.27e-53 172
Msa0091400 MtrunA17_Chr2g0318881 59.341 91 36 1 100 189 21 111 8.17e-33 116
Msa0091400 MtrunA17_Chr2g0318921 63.855 83 29 1 100 181 21 103 3.29e-32 114
Msa0091400 MtrunA17_Chr8g0376351 61.333 75 27 1 119 191 1 75 2.48e-27 100
Msa0091400 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 4.26e-12 64.7
Msa0091400 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.08e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0091400 AT1G08970.3 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0091400 AT1G08970.4 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0091400 AT1G08970.2 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0091400 AT1G08970.1 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0091400 AT1G54830.2 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0091400 AT1G54830.3 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0091400 AT1G54830.1 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0091400 AT1G56170.1 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0091400 AT1G56170.2 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0091400 AT3G48590.1 69.863 146 41 3 83 228 47 189 3.11e-64 201
Msa0091400 AT5G63470.2 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0091400 AT5G63470.1 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0091400 AT5G50480.1 48.276 174 55 7 79 243 35 182 6.37e-44 147
Msa0091400 AT5G27910.1 47.794 136 61 3 78 206 16 148 1.15e-35 125
Msa0091400 AT5G50490.1 39.106 179 90 6 71 244 9 173 3.10e-32 117
Msa0091400 AT5G50470.1 48.148 108 47 2 79 180 44 148 1.20e-28 108
Msa0091400 AT5G38140.1 45.000 100 51 2 80 178 48 144 5.12e-26 101

Find 109 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATATCTTTGATTTCT+TGG 0.152930 1_2:-73517594 Msa0091400:CDS
CTACCAACAACACCCATATT+TGG 0.174332 1_2:+73518064 None:intergenic
TGGAGTTAACCTTGCGTTCT+TGG 0.214555 1_2:-73517682 Msa0091400:CDS
AGATCATTAGATTCTTATTA+TGG 0.246047 1_2:-73517333 Msa0091400:exon
TCATGGCCAAAACCCAAATA+TGG 0.254115 1_2:-73518077 Msa0091400:CDS
AACAACAACTCCAGACCTTT+TGG 0.267438 1_2:-73517862 Msa0091400:CDS
TACAGATGAATAACCAAGTA+TGG 0.276951 1_2:-73517129 Msa0091400:three_prime_UTR
CAACACCCATATTTGGGTTT+TGG 0.289322 1_2:+73518071 None:intergenic
GTCATTGGGTTTGGCTGGTA+TGG 0.344732 1_2:+73518013 None:intergenic
TACCAACAACACCCATATTT+GGG 0.353196 1_2:+73518065 None:intergenic
GTTATATTAGGTGTCTAATA+TGG 0.356708 1_2:-73516953 Msa0091400:three_prime_UTR
TTCTTATTATGGAAGTGTTA+TGG 0.362115 1_2:-73517322 Msa0091400:three_prime_UTR
ATTGTTGTTGCTCCGGTGGT+TGG 0.362576 1_2:+73517363 None:intergenic
TTGTTGTTGCTCCGGTGGTT+GGG 0.366842 1_2:+73517364 None:intergenic
GAAGCATGTGCTGTTATATT+AGG 0.367404 1_2:-73516965 Msa0091400:three_prime_UTR
GGTGACCCAGTCATTGGGTT+TGG 0.375542 1_2:+73518004 None:intergenic
GGGATGGTTGTTCCCTCTGT+TGG 0.377579 1_2:-73517980 Msa0091400:CDS
CAGTAACATTAAGTTCCATT+AGG 0.385447 1_2:+73517163 None:intergenic
GCACAGCAAGCTGGAAATCC+TGG 0.386872 1_2:-73517455 Msa0091400:CDS
GGATGGTTGTTCCCTCTGTT+GGG 0.395560 1_2:-73517979 Msa0091400:CDS
ATCCCAGGTGACCCAGTCAT+TGG 0.407383 1_2:+73517998 None:intergenic
TGAGGCGGCATATAGCAATA+AGG 0.408347 1_2:+73517482 None:intergenic
ACCCAGTCATTGGGTTTGGC+TGG 0.417557 1_2:+73518008 None:intergenic
AACCCAATGACTGGGTCACC+TGG 0.422707 1_2:-73518001 Msa0091400:CDS
TGGAAGTGTTATGGTTAGTA+AGG 0.425704 1_2:-73517313 Msa0091400:three_prime_UTR
GGTGGTTGGGGCCACATTTG+AGG 0.430701 1_2:+73517377 None:intergenic
CACGCCCTTGCAAATACAAC+CGG 0.432250 1_2:+73517719 None:intergenic
TTTACTATGATGTATGGAAA+TGG 0.439756 1_2:+73517224 None:intergenic
CTTGGTTGACATTGTGCCTA+GGG 0.441263 1_2:-73517576 Msa0091400:CDS
AACCCAAATATGGGTGTTGT+TGG 0.445950 1_2:-73518067 Msa0091400:CDS
ACTGATTTCCCCAAAAGGTC+TGG 0.448617 1_2:+73517852 None:intergenic
TCCCAGGTGACCCAGTCATT+GGG 0.450412 1_2:+73517999 None:intergenic
CACAAGATGTTTCTAAGTAC+AGG 0.455147 1_2:-73517081 Msa0091400:three_prime_UTR
AACGCCACAGAAGCACGTTT+CGG 0.455724 1_2:+73519436 None:intergenic
CACATTTGAGGTGCCATGTA+TGG 0.456179 1_2:+73517389 None:intergenic
TCAAATGTGGCCCCAACCAC+CGG 0.464006 1_2:-73517375 Msa0091400:CDS
AGGCGCCGGTTGTATTTGCA+AGG 0.466746 1_2:-73517724 Msa0091400:CDS
TGTTGTTGGTAGTGGACAAA+TGG 0.476420 1_2:-73518053 Msa0091400:CDS
TGTTGTTGCTCCGGTGGTTG+GGG 0.477662 1_2:+73517365 None:intergenic
GTTCCATCAGGTTGACCACC+AGG 0.484332 1_2:+73517947 None:intergenic
CTAATAATGCAAGATCAGAT+TGG 0.485011 1_2:+73517018 None:intergenic
TCGAACACTCTCGTGCAATC+AGG 0.486677 1_2:-73519406 Msa0091400:five_prime_UTR
TCAACCTGATGGAACTCAGC+TGG 0.487041 1_2:-73517939 Msa0091400:CDS
CCAGCAGGCATCGGTCCTCT+TGG 0.488412 1_2:+73517524 None:intergenic
TTGATACTGATTTCCCCAAA+AGG 0.488519 1_2:+73517847 None:intergenic
TTGAGTGGTGATGCTTGCCC+TGG 0.495063 1_2:+73519372 None:intergenic
CCAAGAGGACCGATGCCTGC+TGG 0.496911 1_2:-73517524 Msa0091400:CDS
TGTCCCAGCTGAGTTCCATC+AGG 0.498006 1_2:+73517935 None:intergenic
TCTTGGTTGACATTGTGCCT+AGG 0.499762 1_2:-73517577 Msa0091400:CDS
CCTCAAAATGCACAGCAAGC+TGG 0.503997 1_2:-73517464 Msa0091400:CDS
ATCACACTGAAGAGAACAAA+AGG 0.504661 1_2:-73517658 Msa0091400:CDS
TACCAGCCAAACCCAATGAC+TGG 0.504830 1_2:-73518010 Msa0091400:CDS
GAATCATCATAAACCATACT+TGG 0.506611 1_2:+73517116 None:intergenic
GACTGTGGAGCATACATGTT+GGG 0.509025 1_2:+73517416 None:intergenic
GGCGTGTGAAATGTTCATAT+TGG 0.517805 1_2:-73517702 Msa0091400:CDS
CATTCCGAAACGTGCTTCTG+TGG 0.523815 1_2:-73519440 Msa0091400:five_prime_UTR
CCATCAGCAGGTCCTCCAGC+AGG 0.525310 1_2:+73517509 None:intergenic
TGGTATGGATTAGAACCGTA+TGG 0.527698 1_2:+73518028 None:intergenic
TCTTGATCCTTGCCAAGGGA+AGG 0.527734 1_2:+73517789 None:intergenic
AATATGGGTGTTGTTGGTAG+TGG 0.529665 1_2:-73518061 Msa0091400:CDS
TCATCTTTCAAGTCCTCCCT+AGG 0.530171 1_2:+73517560 None:intergenic
CATGGCCAAAACCCAAATAT+GGG 0.534350 1_2:-73518076 Msa0091400:CDS
ATGGTTAGTAAGGTGAACTT+TGG 0.536359 1_2:-73517303 Msa0091400:three_prime_UTR
GGATTATGCATCATGAGTCC+AGG 0.538672 1_2:+73517437 None:intergenic
CAACCTGATGGAACTCAGCT+GGG 0.543463 1_2:-73517938 Msa0091400:CDS
CAATAAGGAATTCCATCAGC+AGG 0.543484 1_2:+73517497 None:intergenic
GGCGATTGTTGTTGCTCCGG+TGG 0.545817 1_2:+73517359 None:intergenic
AATCTTCTTGATCCTTGCCA+AGG 0.551207 1_2:+73517784 None:intergenic
GATGATATCAGCTGAGGCGC+CGG 0.551962 1_2:-73517738 Msa0091400:CDS
CCTGCTGGAGGACCTGCTGA+TGG 0.553104 1_2:-73517509 Msa0091400:CDS
CAACCTGGTGGTCAACCTGA+TGG 0.553542 1_2:-73517950 Msa0091400:CDS
GAAGGCGATTGTTGTTGCTC+CGG 0.554014 1_2:+73517356 None:intergenic
ATAAGAATCTAATGATCTGA+AGG 0.560767 1_2:+73517338 None:intergenic
GACATTGCAGCAGCAATCAC+AGG 0.562745 1_2:-73517617 Msa0091400:CDS
GAAGATTATGAAGGCTGATG+AGG 0.566353 1_2:-73517768 Msa0091400:CDS
ACCCAATGACTGGGTCACCT+GGG 0.574510 1_2:-73518000 Msa0091400:CDS
GCAGGTCCTCCAGCAGGCAT+CGG 0.575223 1_2:+73517515 None:intergenic
GGTATGGATTAGAACCGTAT+GGG 0.576207 1_2:+73518029 None:intergenic
GGTTGACATTGTGCCTAGGG+AGG 0.582771 1_2:-73517573 Msa0091400:CDS
ATCTTCTTGATCCTTGCCAA+GGG 0.584713 1_2:+73517785 None:intergenic
GTATTGTGATATTGCAGTAG+TGG 0.585313 1_2:-73517043 Msa0091400:three_prime_UTR
TCCACAGTCTCATCCATACA+TGG 0.588194 1_2:-73517402 Msa0091400:CDS
TCCTTGCCAAGGGAAGGCTG+TGG 0.591000 1_2:+73517795 None:intergenic
ACCACAGCCTTCCCTTGGCA+AGG 0.592871 1_2:-73517796 Msa0091400:CDS
GGCGCCGGTTGTATTTGCAA+GGG 0.593275 1_2:-73517723 Msa0091400:CDS
ACAGAGGGAACAACCATCCC+AGG 0.593577 1_2:+73517983 None:intergenic
CGTTTCGGAATGTTCTCGTA+CGG 0.595038 1_2:+73519451 None:intergenic
AAGGATCAAGAAGATTATGA+AGG 0.595150 1_2:-73517777 Msa0091400:CDS
AGTGGACAAATGGCCCCATA+CGG 0.596575 1_2:-73518043 Msa0091400:CDS
AGAATGGATCATCAAGGTCA+TGG 0.604516 1_2:-73518094 Msa0091400:CDS
AATGACTGGGTCACCTGGGA+TGG 0.607597 1_2:-73517996 Msa0091400:CDS
TGAAACAGAATGGATCATCA+AGG 0.611963 1_2:-73518100 Msa0091400:intron
TCTGTTGGGACTATTCAACC+TGG 0.614722 1_2:-73517965 Msa0091400:CDS
CATACATGGCACCTCAAATG+TGG 0.616231 1_2:-73517388 Msa0091400:CDS
AGTTGGTACCTTGTGTTGAG+TGG 0.622959 1_2:+73519357 None:intergenic
ACCAGCCAAACCCAATGACT+GGG 0.624633 1_2:-73518009 Msa0091400:CDS
AGAGGACCGATGCCTGCTGG+AGG 0.625695 1_2:-73517521 Msa0091400:CDS
AAGCATCACCACTCAACACA+AGG 0.628345 1_2:-73519365 Msa0091400:intron
TCAGTATCAAGAAATTGAGA+AGG 0.629465 1_2:-73517834 Msa0091400:CDS
AGTGTGATTCCAAGAACGCA+AGG 0.629484 1_2:+73517673 None:intergenic
TTGAGAACACACTGAACATG+AGG 0.648120 1_2:-73516930 Msa0091400:three_prime_UTR
GGTTGAATAGTCCCAACAGA+GGG 0.660370 1_2:+73517968 None:intergenic
TGTGAAGATGATATCAGCTG+AGG 0.675937 1_2:-73517744 Msa0091400:CDS
GAAGTGCTTGCATTACCAAG+AGG 0.676504 1_2:-73517539 Msa0091400:CDS
GTTGGGACTATTCAACCTGG+TGG 0.686021 1_2:-73517962 Msa0091400:CDS
AGACTGTGGAGCATACATGT+TGG 0.689302 1_2:+73517415 None:intergenic
GTATGGATTAGAACCGTATG+GGG 0.698616 1_2:+73518030 None:intergenic
GCCATGTATGGATGAGACTG+TGG 0.701999 1_2:+73517401 None:intergenic
AGGTTGAATAGTCCCAACAG+AGG 0.742829 1_2:+73517967 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGATTTTTTTTTTTTTTTT+AGG - chr1_2:73517431-73517450 Msa0091400:CDS 10.0%
!!! GGATTTTTTTTTTTTTTTTA+GGG - chr1_2:73517432-73517451 Msa0091400:CDS 10.0%
!!! AAATGTTATATGATATTTTA+CGG - chr1_2:73517657-73517676 Msa0091400:CDS 10.0%
!!! TTTTTTTTTTTTTTTAGGGT+TGG - chr1_2:73517436-73517455 Msa0091400:CDS 15.0%
!! CAGTATATATTATAACTATA+TGG + chr1_2:73517462-73517481 None:intergenic 15.0%
!! TAATATATACTGTAAATTGT+TGG - chr1_2:73517470-73517489 Msa0091400:CDS 15.0%
!! GTAAAATATCATATAACATT+TGG + chr1_2:73517658-73517677 None:intergenic 15.0%
!!! TTTAGGAGAAAAATTAATTT+TGG - chr1_2:73517785-73517804 Msa0091400:CDS 15.0%
!!! TTAGGAGAAAAATTAATTTT+GGG - chr1_2:73517786-73517805 Msa0091400:CDS 15.0%
!! AATCGAATTACATAATGAAA+AGG + chr1_2:73517036-73517055 None:intergenic 20.0%
!!! TTGTTTAGTTTTTCTTGTTA+CGG - chr1_2:73517054-73517073 Msa0091400:three_prime_UTR 20.0%
!! AGCAAATATCTAATATTTCA+TGG + chr1_2:73517137-73517156 None:intergenic 20.0%
!! TGAAATATTAGATATTTGCT+TGG - chr1_2:73517137-73517156 Msa0091400:three_prime_UTR 20.0%
!! AGCAGATTTATTTATGAATA+TGG - chr1_2:73517189-73517208 Msa0091400:three_prime_UTR 20.0%
!!! TATTTCTTTTATTCCATTCA+AGG - chr1_2:73517367-73517386 Msa0091400:CDS 20.0%
!! AGATCATTAGATTCTTATTA+TGG - chr1_2:73519012-73519031 Msa0091400:intron 20.0%
!!! TGCAATAAATTAGTTTTACA+AGG - chr1_2:73519065-73519084 Msa0091400:intron 20.0%
!!! GCAATAAATTAGTTTTACAA+GGG - chr1_2:73519066-73519085 Msa0091400:intron 20.0%
!!! TTAAGTTTTACTATGATGTA+TGG + chr1_2:73519130-73519149 None:intergenic 20.0%
!!! TAATAATTTTGTATGTTGTG+TGG - chr1_2:73519358-73519377 Msa0091400:intron 20.0%
! AATATTAGATATTTGCTTGG+TGG - chr1_2:73517140-73517159 Msa0091400:three_prime_UTR 25.0%
! TTATGAATATGGTGAGAATT+AGG - chr1_2:73517200-73517219 Msa0091400:three_prime_UTR 25.0%
! TATGAATATGGTGAGAATTA+GGG - chr1_2:73517201-73517220 Msa0091400:three_prime_UTR 25.0%
! TTCCAAATACATGATTGATT+TGG - chr1_2:73517339-73517358 Msa0091400:exon 25.0%
! AACACAAAAAAAACCTTGAA+TGG + chr1_2:73517383-73517402 None:intergenic 25.0%
!!! GGTTTTTTTTGTGTTTTTCA+TGG - chr1_2:73517388-73517407 Msa0091400:CDS 25.0%
! ATATACTGTAAATTGTTGGT+TGG - chr1_2:73517474-73517493 Msa0091400:CDS 25.0%
! ATGTTTAATATGTTGCAAAG+AGG - chr1_2:73517581-73517600 Msa0091400:CDS 25.0%
! TTTAATGCCAATAGCTATTT+AGG - chr1_2:73517768-73517787 Msa0091400:CDS 25.0%
!! TTTTTCTCCTAAATAGCTAT+TGG + chr1_2:73517778-73517797 None:intergenic 25.0%
!!! GAGAAAAATTAATTTTGGGT+AGG - chr1_2:73517790-73517809 Msa0091400:CDS 25.0%
! ATAACTTTGTACAAGTGTAT+TGG - chr1_2:73517902-73517921 Msa0091400:CDS 25.0%
!! AATTAAGATTTGTTCGAGTT+TGG - chr1_2:73517929-73517948 Msa0091400:CDS 25.0%
! ACTTTAGTACAAAGTTATGT+CGG + chr1_2:73518048-73518067 None:intergenic 25.0%
! AACTTTGTACTAAAGTGTAT+TGG - chr1_2:73518052-73518071 Msa0091400:CDS 25.0%
!!! ATGTGCATATTGTTTTGTTT+TGG - chr1_2:73518182-73518201 Msa0091400:intron 25.0%
!! AACTGATATCTTTGATTTCT+TGG - chr1_2:73518751-73518770 Msa0091400:intron 25.0%
! ATAAGAATCTAATGATCTGA+AGG + chr1_2:73519010-73519029 None:intergenic 25.0%
!!! TTCTTATTATGGAAGTGTTA+TGG - chr1_2:73519023-73519042 Msa0091400:intron 25.0%
! TTTACTATGATGTATGGAAA+TGG + chr1_2:73519124-73519143 None:intergenic 25.0%
!!! TTCAATGTCATTTTTCCTAA+TGG - chr1_2:73519167-73519186 Msa0091400:intron 25.0%
! GTTATATTAGGTGTCTAATA+TGG - chr1_2:73519392-73519411 Msa0091400:five_prime_UTR 25.0%
!!! AATCAGGTTATTGATTTTCC+AGG - chr1_2:73516955-73516974 Msa0091400:three_prime_UTR 30.0%
!!! ATCAGGTTATTGATTTTCCA+GGG - chr1_2:73516956-73516975 Msa0091400:three_prime_UTR 30.0%
! CTTGTTATGCTTGATGTATA+TGG - chr1_2:73517267-73517286 Msa0091400:three_prime_UTR 30.0%
CTCCAAATCAATCATGTATT+TGG + chr1_2:73517344-73517363 None:intergenic 30.0%
!! CAAATACATGATTGATTTGG+AGG - chr1_2:73517342-73517361 Msa0091400:exon 30.0%
! TAAAGATTTTGTTCAAGTCC+TGG - chr1_2:73518080-73518099 Msa0091400:CDS 30.0%
ACAATTACACGTGAACAAAA+GGG + chr1_2:73518156-73518175 None:intergenic 30.0%
AACAATTACACGTGAACAAA+AGG + chr1_2:73518157-73518176 None:intergenic 30.0%
TGATTTCACTTGAAACAGAA+TGG - chr1_2:73518235-73518254 Msa0091400:intron 30.0%
TCAGTATCAAGAAATTGAGA+AGG - chr1_2:73518511-73518530 Msa0091400:intron 30.0%
AAGGATCAAGAAGATTATGA+AGG - chr1_2:73518568-73518587 Msa0091400:intron 30.0%
! TGCAATGTCATTCTTTTGAA+GGG + chr1_2:73518717-73518736 None:intergenic 30.0%
CAGTAACATTAAGTTCCATT+AGG + chr1_2:73519185-73519204 None:intergenic 30.0%
TACAGATGAATAACCAAGTA+TGG - chr1_2:73519216-73519235 Msa0091400:intron 30.0%
GAATCATCATAAACCATACT+TGG + chr1_2:73519232-73519251 None:intergenic 30.0%
CTAATAATGCAAGATCAGAT+TGG + chr1_2:73519330-73519349 None:intergenic 30.0%
!! CACTAAATTCTGGGGTTAAT+CGG + chr1_2:73517107-73517126 None:intergenic 35.0%
!! TTTGCTTGGTGGTTTTTATG+CGG - chr1_2:73517151-73517170 Msa0091400:three_prime_UTR 35.0%
!! AAATATACACAAGTGCTGGA+TGG + chr1_2:73517320-73517339 None:intergenic 35.0%
TGGAAAATATACACAAGTGC+TGG + chr1_2:73517324-73517343 None:intergenic 35.0%
! TTTTGTGTTTTTCATGGTGC+TGG - chr1_2:73517394-73517413 Msa0091400:CDS 35.0%
! TTTGTGTTTTTCATGGTGCT+GGG - chr1_2:73517395-73517414 Msa0091400:CDS 35.0%
!! TATAACATTTGGTAGCGTAG+TGG + chr1_2:73517647-73517666 None:intergenic 35.0%
!! AATTAATTTTGGGTAGGCCA+TGG - chr1_2:73517796-73517815 Msa0091400:CDS 35.0%
AAACAATGTTTATCGCGTAG+CGG + chr1_2:73517879-73517898 None:intergenic 35.0%
CAATTACACGTGAACAAAAG+GGG + chr1_2:73518155-73518174 None:intergenic 35.0%
TGAAACAGAATGGATCATCA+AGG - chr1_2:73518245-73518264 Msa0091400:intron 35.0%
TACCAACAACACCCATATTT+GGG + chr1_2:73518283-73518302 None:intergenic 35.0%
!! TTGATACTGATTTCCCCAAA+AGG + chr1_2:73518501-73518520 None:intergenic 35.0%
ATCACACTGAAGAGAACAAA+AGG - chr1_2:73518687-73518706 Msa0091400:intron 35.0%
! CTGCAATGTCATTCTTTTGA+AGG + chr1_2:73518718-73518737 None:intergenic 35.0%
!! TGGAAGTGTTATGGTTAGTA+AGG - chr1_2:73519032-73519051 Msa0091400:intron 35.0%
ATGGTTAGTAAGGTGAACTT+TGG - chr1_2:73519042-73519061 Msa0091400:intron 35.0%
! CACAAGATGTTTCTAAGTAC+AGG - chr1_2:73519264-73519283 Msa0091400:intron 35.0%
GTATTGTGATATTGCAGTAG+TGG - chr1_2:73519302-73519321 Msa0091400:intron 35.0%
! GAAGCATGTGCTGTTATATT+AGG - chr1_2:73519380-73519399 Msa0091400:five_prime_UTR 35.0%
ACCCCAGAATTTAGTGTTTG+TGG - chr1_2:73517111-73517130 Msa0091400:three_prime_UTR 40.0%
GCCACAAACACTAAATTCTG+GGG + chr1_2:73517115-73517134 None:intergenic 40.0%
GGCCACAAACACTAAATTCT+GGG + chr1_2:73517116-73517135 None:intergenic 40.0%
TGGCCACAAACACTAAATTC+TGG + chr1_2:73517117-73517136 None:intergenic 40.0%
!! GTTGGTTGGTAAGACTTGAT+TGG - chr1_2:73517488-73517507 Msa0091400:CDS 40.0%
TGGTATAATTGTCGAGACGA+AGG - chr1_2:73517508-73517527 Msa0091400:CDS 40.0%
AACAATGTTTATCGCGTAGC+GGG + chr1_2:73517878-73517897 None:intergenic 40.0%
! TGTCGGTGTTTATCATGTAG+TGG + chr1_2:73518031-73518050 None:intergenic 40.0%
CTAGAATGCTATAGCATACC+AGG + chr1_2:73518101-73518120 None:intergenic 40.0%
TCAATTCCTGCACTTCACAT+GGG + chr1_2:73518209-73518228 None:intergenic 40.0%
ATCAATTCCTGCACTTCACA+TGG + chr1_2:73518210-73518229 None:intergenic 40.0%
AGAATGGATCATCAAGGTCA+TGG - chr1_2:73518251-73518270 Msa0091400:intron 40.0%
TCATGGCCAAAACCCAAATA+TGG - chr1_2:73518268-73518287 Msa0091400:intron 40.0%
CATGGCCAAAACCCAAATAT+GGG - chr1_2:73518269-73518288 Msa0091400:intron 40.0%
!! CAACACCCATATTTGGGTTT+TGG + chr1_2:73518277-73518296 None:intergenic 40.0%
! AACCCAAATATGGGTGTTGT+TGG - chr1_2:73518278-73518297 Msa0091400:intron 40.0%
CTACCAACAACACCCATATT+TGG + chr1_2:73518284-73518303 None:intergenic 40.0%
!! AATATGGGTGTTGTTGGTAG+TGG - chr1_2:73518284-73518303 Msa0091400:intron 40.0%
!! TGTTGTTGGTAGTGGACAAA+TGG - chr1_2:73518292-73518311 Msa0091400:intron 40.0%
!! GTATGGATTAGAACCGTATG+GGG + chr1_2:73518318-73518337 None:intergenic 40.0%
!! GGTATGGATTAGAACCGTAT+GGG + chr1_2:73518319-73518338 None:intergenic 40.0%
!! TGGTATGGATTAGAACCGTA+TGG + chr1_2:73518320-73518339 None:intergenic 40.0%
! AACAACAACTCCAGACCTTT+TGG - chr1_2:73518483-73518502 Msa0091400:intron 40.0%
! ACAACAACTCCAGACCTTTT+GGG - chr1_2:73518484-73518503 Msa0091400:intron 40.0%
ATCTTCTTGATCCTTGCCAA+GGG + chr1_2:73518563-73518582 None:intergenic 40.0%
AATCTTCTTGATCCTTGCCA+AGG + chr1_2:73518564-73518583 None:intergenic 40.0%
GAAGATTATGAAGGCTGATG+AGG - chr1_2:73518577-73518596 Msa0091400:intron 40.0%
TGTGAAGATGATATCAGCTG+AGG - chr1_2:73518601-73518620 Msa0091400:intron 40.0%
GGCGTGTGAAATGTTCATAT+TGG - chr1_2:73518643-73518662 Msa0091400:intron 40.0%
CAATAAGGAATTCCATCAGC+AGG + chr1_2:73518851-73518870 None:intergenic 40.0%
TTGAGAACACACTGAACATG+AGG - chr1_2:73519415-73519434 Msa0091400:five_prime_UTR 40.0%
CGTTTCGGAATGTTCTCGTA+CGG + chr1_2:73516897-73516916 None:intergenic 45.0%
AAGCATCACCACTCAACACA+AGG - chr1_2:73516980-73516999 Msa0091400:three_prime_UTR 45.0%
! AGTTGGTACCTTGTGTTGAG+TGG + chr1_2:73516991-73517010 None:intergenic 45.0%
AGTACGCGAATTGCAAGAGT+TGG + chr1_2:73517008-73517027 None:intergenic 45.0%
!! TGTTTTTCATGGTGCTGGGA+AGG - chr1_2:73517399-73517418 Msa0091400:CDS 45.0%
!! GTTTTTCATGGTGCTGGGAA+GGG - chr1_2:73517400-73517419 Msa0091400:CDS 45.0%
!! TTTTTCATGGTGCTGGGAAG+GGG - chr1_2:73517401-73517420 Msa0091400:CDS 45.0%
ACAATGTTTATCGCGTAGCG+GGG + chr1_2:73517877-73517896 None:intergenic 45.0%
TCCACAATTGACACCATTGG+CGG + chr1_2:73517981-73518000 None:intergenic 45.0%
CAGTCCACAATTGACACCAT+TGG + chr1_2:73517984-73518003 None:intergenic 45.0%
! ATGGTGTCAATTGTGGACTG+CGG - chr1_2:73517984-73518003 Msa0091400:CDS 45.0%
CAATTCCTGCACTTCACATG+GGG + chr1_2:73518208-73518227 None:intergenic 45.0%
GGTTGAATAGTCCCAACAGA+GGG + chr1_2:73518380-73518399 None:intergenic 45.0%
AGGTTGAATAGTCCCAACAG+AGG + chr1_2:73518381-73518400 None:intergenic 45.0%
TCTGTTGGGACTATTCAACC+TGG - chr1_2:73518380-73518399 Msa0091400:intron 45.0%
! CAACAACTCCAGACCTTTTG+GGG - chr1_2:73518485-73518504 Msa0091400:intron 45.0%
! ACTGATTTCCCCAAAAGGTC+TGG + chr1_2:73518496-73518515 None:intergenic 45.0%
TGGAGTTAACCTTGCGTTCT+TGG - chr1_2:73518663-73518682 Msa0091400:intron 45.0%
AGTGTGATTCCAAGAACGCA+AGG + chr1_2:73518675-73518694 None:intergenic 45.0%
TCTTGGTTGACATTGTGCCT+AGG - chr1_2:73518768-73518787 Msa0091400:intron 45.0%
CTTGGTTGACATTGTGCCTA+GGG - chr1_2:73518769-73518788 Msa0091400:intron 45.0%
! TCATCTTTCAAGTCCTCCCT+AGG + chr1_2:73518788-73518807 None:intergenic 45.0%
! GAAGTGCTTGCATTACCAAG+AGG - chr1_2:73518806-73518825 Msa0091400:intron 45.0%
TGAGGCGGCATATAGCAATA+AGG + chr1_2:73518866-73518885 None:intergenic 45.0%
GGATTATGCATCATGAGTCC+AGG + chr1_2:73518911-73518930 None:intergenic 45.0%
GACTGTGGAGCATACATGTT+GGG + chr1_2:73518932-73518951 None:intergenic 45.0%
AGACTGTGGAGCATACATGT+TGG + chr1_2:73518933-73518952 None:intergenic 45.0%
TCCACAGTCTCATCCATACA+TGG - chr1_2:73518943-73518962 Msa0091400:intron 45.0%
! CACATTTGAGGTGCCATGTA+TGG + chr1_2:73518959-73518978 None:intergenic 45.0%
CATACATGGCACCTCAAATG+TGG - chr1_2:73518957-73518976 Msa0091400:intron 45.0%
CATTCCGAAACGTGCTTCTG+TGG - chr1_2:73516905-73516924 Msa0091400:three_prime_UTR 50.0%
! AACGCCACAGAAGCACGTTT+CGG + chr1_2:73516912-73516931 None:intergenic 50.0%
TCGAACACTCTCGTGCAATC+AGG - chr1_2:73516939-73516958 Msa0091400:three_prime_UTR 50.0%
ATCCGCAAACGACACCAGTA+GGG + chr1_2:73517830-73517849 None:intergenic 50.0%
AATCCGCAAACGACACCAGT+AGG + chr1_2:73517831-73517850 None:intergenic 50.0%
TCAAATCACAGCTCCGCCAA+TGG - chr1_2:73517965-73517984 Msa0091400:CDS 50.0%
! TCCGCCAATGGTGTCAATTG+TGG - chr1_2:73517977-73517996 Msa0091400:CDS 50.0%
AGTGGACAAATGGCCCCATA+CGG - chr1_2:73518302-73518321 Msa0091400:intron 50.0%
GTCATTGGGTTTGGCTGGTA+TGG + chr1_2:73518335-73518354 None:intergenic 50.0%
TACCAGCCAAACCCAATGAC+TGG - chr1_2:73518335-73518354 Msa0091400:intron 50.0%
ACCAGCCAAACCCAATGACT+GGG - chr1_2:73518336-73518355 Msa0091400:intron 50.0%
GGATGGTTGTTCCCTCTGTT+GGG - chr1_2:73518366-73518385 Msa0091400:intron 50.0%
GTTGGGACTATTCAACCTGG+TGG - chr1_2:73518383-73518402 Msa0091400:intron 50.0%
TCAACCTGATGGAACTCAGC+TGG - chr1_2:73518406-73518425 Msa0091400:intron 50.0%
CAACCTGATGGAACTCAGCT+GGG - chr1_2:73518407-73518426 Msa0091400:intron 50.0%
CAAAAACCACAGCCTTCCCT+TGG - chr1_2:73518544-73518563 Msa0091400:intron 50.0%
TCTTGATCCTTGCCAAGGGA+AGG + chr1_2:73518559-73518578 None:intergenic 50.0%
CACGCCCTTGCAAATACAAC+CGG + chr1_2:73518629-73518648 None:intergenic 50.0%
GACATTGCAGCAGCAATCAC+AGG - chr1_2:73518728-73518747 Msa0091400:intron 50.0%
!!! GCTTGCTGTGCATTTTGAGG+CGG + chr1_2:73518881-73518900 None:intergenic 50.0%
!!! CCAGCTTGCTGTGCATTTTG+AGG + chr1_2:73518884-73518903 None:intergenic 50.0%
CCTCAAAATGCACAGCAAGC+TGG - chr1_2:73518881-73518900 Msa0091400:intron 50.0%
GCCATGTATGGATGAGACTG+TGG + chr1_2:73518947-73518966 None:intergenic 50.0%
! TTGTTGTTGCTCCGGTGGTT+GGG + chr1_2:73518984-73519003 None:intergenic 50.0%
! ATTGTTGTTGCTCCGGTGGT+TGG + chr1_2:73518985-73519004 None:intergenic 50.0%
! GAAGGCGATTGTTGTTGCTC+CGG + chr1_2:73518992-73519011 None:intergenic 50.0%
! TTGAGTGGTGATGCTTGCCC+TGG + chr1_2:73516976-73516995 None:intergenic 55.0%
! TTTTGTCCCTCGTAGGTCGC+AGG + chr1_2:73517614-73517633 None:intergenic 55.0%
GACCTACGAGGGACAAAACG+CGG - chr1_2:73517616-73517635 Msa0091400:CDS 55.0%
ACCTACGAGGGACAAAACGC+GGG - chr1_2:73517617-73517636 Msa0091400:CDS 55.0%
TCCGCAAACGACACCAGTAG+GGG + chr1_2:73517829-73517848 None:intergenic 55.0%
!! CTGGTGTCGTTTGCGGATTG+CGG - chr1_2:73517832-73517851 Msa0091400:CDS 55.0%
TTTGGCCCCATGTGAAGTGC+AGG - chr1_2:73518200-73518219 Msa0091400:intron 55.0%
ACCCAGTCATTGGGTTTGGC+TGG + chr1_2:73518340-73518359 None:intergenic 55.0%
GGTGACCCAGTCATTGGGTT+TGG + chr1_2:73518344-73518363 None:intergenic 55.0%
AACCCAATGACTGGGTCACC+TGG - chr1_2:73518344-73518363 Msa0091400:intron 55.0%
ACCCAATGACTGGGTCACCT+GGG - chr1_2:73518345-73518364 Msa0091400:intron 55.0%
TCCCAGGTGACCCAGTCATT+GGG + chr1_2:73518349-73518368 None:intergenic 55.0%
ATCCCAGGTGACCCAGTCAT+TGG + chr1_2:73518350-73518369 None:intergenic 55.0%
AATGACTGGGTCACCTGGGA+TGG - chr1_2:73518349-73518368 Msa0091400:intron 55.0%
ACAGAGGGAACAACCATCCC+AGG + chr1_2:73518365-73518384 None:intergenic 55.0%
GGGATGGTTGTTCCCTCTGT+TGG - chr1_2:73518365-73518384 Msa0091400:intron 55.0%
CAACCTGGTGGTCAACCTGA+TGG - chr1_2:73518395-73518414 Msa0091400:intron 55.0%
!! GTTCCATCAGGTTGACCACC+AGG + chr1_2:73518401-73518420 None:intergenic 55.0%
TGTCCCAGCTGAGTTCCATC+AGG + chr1_2:73518413-73518432 None:intergenic 55.0%
GATGATATCAGCTGAGGCGC+CGG - chr1_2:73518607-73518626 Msa0091400:intron 55.0%
!! AGGCGCCGGTTGTATTTGCA+AGG - chr1_2:73518621-73518640 Msa0091400:intron 55.0%
!! GGCGCCGGTTGTATTTGCAA+GGG - chr1_2:73518622-73518641 Msa0091400:intron 55.0%
GGTTGACATTGTGCCTAGGG+AGG - chr1_2:73518772-73518791 Msa0091400:intron 55.0%
GCACAGCAAGCTGGAAATCC+TGG - chr1_2:73518890-73518909 Msa0091400:intron 55.0%
TCAAATGTGGCCCCAACCAC+CGG - chr1_2:73518970-73518989 Msa0091400:intron 55.0%
TGTTGTTGCTCCGGTGGTTG+GGG + chr1_2:73518983-73519002 None:intergenic 55.0%
TGCTGGGAAGGGGTGAAGCT+AGG - chr1_2:73517411-73517430 Msa0091400:CDS 60.0%
ACTCGATCCTGCGACCTACG+AGG - chr1_2:73517604-73517623 Msa0091400:CDS 60.0%
CTCGATCCTGCGACCTACGA+GGG - chr1_2:73517605-73517624 Msa0091400:CDS 60.0%
! ACCCGCGTTTTGTCCCTCGT+AGG + chr1_2:73517621-73517640 None:intergenic 60.0%
CCGCAAACGACACCAGTAGG+GGG + chr1_2:73517828-73517847 None:intergenic 60.0%
!! CCCCCTACTGGTGTCGTTTG+CGG - chr1_2:73517825-73517844 Msa0091400:CDS 60.0%
ACCACAGCCTTCCCTTGGCA+AGG - chr1_2:73518549-73518568 Msa0091400:intron 60.0%
TCCTTGCCAAGGGAAGGCTG+TGG + chr1_2:73518553-73518572 None:intergenic 60.0%
GGTGGTTGGGGCCACATTTG+AGG + chr1_2:73518971-73518990 None:intergenic 60.0%
! GGCGATTGTTGTTGCTCCGG+TGG + chr1_2:73518989-73519008 None:intergenic 60.0%
CCAGTAGGGGGAGCTAGCCA+TGG + chr1_2:73517816-73517835 None:intergenic 65.0%
! CCATGGCTAGCTCCCCCTAC+TGG - chr1_2:73517813-73517832 Msa0091400:CDS 65.0%
!! CCAGCAGGCATCGGTCCTCT+TGG + chr1_2:73518824-73518843 None:intergenic 65.0%
CCAAGAGGACCGATGCCTGC+TGG - chr1_2:73518821-73518840 Msa0091400:intron 65.0%
AGAGGACCGATGCCTGCTGG+AGG - chr1_2:73518824-73518843 Msa0091400:intron 65.0%
GCAGGTCCTCCAGCAGGCAT+CGG + chr1_2:73518833-73518852 None:intergenic 65.0%
CCATCAGCAGGTCCTCCAGC+AGG + chr1_2:73518839-73518858 None:intergenic 65.0%
CCTGCTGGAGGACCTGCTGA+TGG - chr1_2:73518836-73518855 Msa0091400:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 73516890 73519477 73516890 ID=Msa0091400;Name=Msa0091400
chr1_2 mRNA 73516890 73519477 73516890 ID=Msa0091400-mRNA-1;Parent=Msa0091400;Name=Msa0091400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=112|0|0.5|1|0|0.5|2|456|255
chr1_2 exon 73516890 73518113 73516890 ID=Msa0091400-mRNA-1:exon:25805;Parent=Msa0091400-mRNA-1
chr1_2 exon 73519366 73519477 73519366 ID=Msa0091400-mRNA-1:exon:25804;Parent=Msa0091400-mRNA-1
chr1_2 five_prime_UTR 73519366 73519477 73519366 ID=Msa0091400-mRNA-1:five_prime_utr;Parent=Msa0091400-mRNA-1
chr1_2 CDS 73517346 73518113 73517346 ID=Msa0091400-mRNA-1:cds;Parent=Msa0091400-mRNA-1
chr1_2 three_prime_UTR 73516890 73517345 73516890 ID=Msa0091400-mRNA-1:three_prime_utr;Parent=Msa0091400-mRNA-1
Gene Sequence

>Msa0091400

ATGGATCATCAAGGTCATGGCCAAAACCCAAATATGGGTGTTGTTGGTAGTGGACAAATGGCCCCATACGGTTCTAATCCATACCAGCCAAACCCAATGACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGACTATTCAACCTGGTGGTCAACCTGATGGAACTCAGCTGGGACAACATCAGCTTGCTTATCAGCACATTCATCAGCAGCAACAGCAGCAGCAACAACAACAACTCCAGACCTTTTGGGGAAATCAGTATCAAGAAATTGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCTGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGTGAAATGTTCATATTGGAGTTAACCTTGCGTTCTTGGAATCACACTGAAGAGAACAAAAGGCGAACCCTTCAAAAGAATGACATTGCAGCAGCAATCACAGGAACTGATATCTTTGATTTCTTGGTTGACATTGTGCCTAGGGAGGACTTGAAAGATGAAGTGCTTGCATTACCAAGAGGACCGATGCCTGCTGGAGGACCTGCTGATGGAATTCCTTATTGCTATATGCCGCCTCAAAATGCACAGCAAGCTGGAAATCCTGGACTCATGATGCATAATCCCAACATGTATGCTCCACAGTCTCATCCATACATGGCACCTCAAATGTGGCCCCAACCACCGGAGCAACAACAATCGCCTTCAGATCATTAG
Protein sequence

>Msa0091400

MDHQGHGQNPNMGVVGSGQMAPYGSNPYQPNPMTGSPGMVVPSVGTIQPGGQPDGTQLGQHQLAYQHIHQQQQQQQQQQLQTFWGNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVKMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITGTDIFDFLVDIVPREDLKDEVLALPRGPMPAGGPADGIPYCYMPPQNAQQAGNPGLMMHNPNMYAPQSHPYMAPQMWPQPPEQQQSPSDH*