AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0168810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168810 MtrunA17_Chr1g0191681 98.828 256 2 1 1 255 1 256 0.0 518
Msa0168810 MtrunA17_Chr7g0272991 81.154 260 44 2 1 255 1 260 8.29e-145 405
Msa0168810 MtrunA17_Chr2g0310741 58.065 217 75 8 16 226 20 226 1.33e-69 215
Msa0168810 MtrunA17_Chr3g0132191 82.524 103 18 0 83 185 37 139 1.84e-58 185
Msa0168810 MtrunA17_Chr2g0288481 50.000 184 70 7 82 254 66 238 2.27e-53 172
Msa0168810 MtrunA17_Chr2g0318881 59.341 91 36 1 100 189 21 111 8.17e-33 116
Msa0168810 MtrunA17_Chr2g0318921 63.855 83 29 1 100 181 21 103 3.29e-32 114
Msa0168810 MtrunA17_Chr8g0376351 61.333 75 27 1 119 191 1 75 2.48e-27 100
Msa0168810 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 4.26e-12 64.7
Msa0168810 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.08e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168810 AT1G08970.3 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0168810 AT1G08970.4 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0168810 AT1G08970.2 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0168810 AT1G08970.1 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0168810 AT1G54830.2 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0168810 AT1G54830.3 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0168810 AT1G54830.1 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0168810 AT1G56170.1 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0168810 AT1G56170.2 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0168810 AT3G48590.1 69.863 146 41 3 83 228 47 189 3.11e-64 201
Msa0168810 AT5G63470.2 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0168810 AT5G63470.1 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0168810 AT5G50480.1 48.276 174 55 7 79 243 35 182 6.37e-44 147
Msa0168810 AT5G27910.1 47.794 136 61 3 78 206 16 148 1.15e-35 125
Msa0168810 AT5G50490.1 39.106 179 90 6 71 244 9 173 3.10e-32 117
Msa0168810 AT5G50470.1 48.148 108 47 2 79 180 44 148 1.20e-28 108
Msa0168810 AT5G38140.1 45.000 100 51 2 80 178 48 144 5.12e-26 101

Find 109 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATATCTTTGATTTCT+TGG 0.152930 1_4:+51754441 Msa0168810:CDS
CTACCAACAACACCCATATT+TGG 0.174332 1_4:-51753971 None:intergenic
TGGAGTTAACCTTGCGTTCT+TGG 0.214555 1_4:+51754353 Msa0168810:CDS
AGATCATTAGATTCTTATTA+TGG 0.246047 1_4:+51754702 Msa0168810:exon
TCATGGCCAAAACCCAAATA+TGG 0.254115 1_4:+51753958 Msa0168810:CDS
AACAACAACTCCAGACCTTT+TGG 0.267438 1_4:+51754173 Msa0168810:CDS
TACAGATGAATAACCAAGTA+TGG 0.276951 1_4:+51754906 Msa0168810:three_prime_UTR
CAACACCCATATTTGGGTTT+TGG 0.289322 1_4:-51753964 None:intergenic
GTCATTGGGTTTGGCTGGTA+TGG 0.344732 1_4:-51754022 None:intergenic
TACCAACAACACCCATATTT+GGG 0.353196 1_4:-51753970 None:intergenic
GTTATATTAGGTGTCTAATA+TGG 0.356708 1_4:+51755082 Msa0168810:three_prime_UTR
TTCTTATTATGGAAGTGTTA+TGG 0.362115 1_4:+51754713 Msa0168810:three_prime_UTR
ATTGTTGTTGCTCCGGTGGT+TGG 0.362576 1_4:-51754672 None:intergenic
TTGTTGTTGCTCCGGTGGTT+GGG 0.366842 1_4:-51754671 None:intergenic
GAAGCATGTGCTGTTATATT+AGG 0.367404 1_4:+51755070 Msa0168810:three_prime_UTR
GGTGACCCAGTCATTGGGTT+TGG 0.375542 1_4:-51754031 None:intergenic
GGGATGGTTGTTCCCTCTGT+TGG 0.377579 1_4:+51754055 Msa0168810:CDS
CAGTAACATTAAGTTCCATT+AGG 0.385447 1_4:-51754872 None:intergenic
GCACAGCAAGCTGGAAATCC+TGG 0.386872 1_4:+51754580 Msa0168810:CDS
GGATGGTTGTTCCCTCTGTT+GGG 0.395560 1_4:+51754056 Msa0168810:CDS
ATCCCAGGTGACCCAGTCAT+TGG 0.407383 1_4:-51754037 None:intergenic
TGAGGCGGCATATAGCAATA+AGG 0.408347 1_4:-51754553 None:intergenic
ACCCAGTCATTGGGTTTGGC+TGG 0.417557 1_4:-51754027 None:intergenic
AACCCAATGACTGGGTCACC+TGG 0.422707 1_4:+51754034 Msa0168810:CDS
TGGAAGTGTTATGGTTAGTA+AGG 0.425704 1_4:+51754722 Msa0168810:three_prime_UTR
GGTGGTTGGGGCCACATTTG+AGG 0.430701 1_4:-51754658 None:intergenic
CACGCCCTTGCAAATACAAC+CGG 0.432250 1_4:-51754316 None:intergenic
TTTACTATGATGTATGGAAA+TGG 0.439756 1_4:-51754811 None:intergenic
CTTGGTTGACATTGTGCCTA+GGG 0.441263 1_4:+51754459 Msa0168810:CDS
AACCCAAATATGGGTGTTGT+TGG 0.445950 1_4:+51753968 Msa0168810:CDS
ACTGATTTCCCCAAAAGGTC+TGG 0.448617 1_4:-51754183 None:intergenic
TCCCAGGTGACCCAGTCATT+GGG 0.450412 1_4:-51754036 None:intergenic
CACAAGATGTTTCTAAGTAC+AGG 0.455147 1_4:+51754954 Msa0168810:three_prime_UTR
AACGCCACAGAAGCACGTTT+CGG 0.455724 1_4:-51752601 None:intergenic
CACATTTGAGGTGCCATGTA+TGG 0.456179 1_4:-51754646 None:intergenic
TCAAATGTGGCCCCAACCAC+CGG 0.464006 1_4:+51754660 Msa0168810:CDS
AGGCGCCGGTTGTATTTGCA+AGG 0.466746 1_4:+51754311 Msa0168810:CDS
TGTTGTTGGTAGTGGACAAA+TGG 0.476420 1_4:+51753982 Msa0168810:CDS
TGTTGTTGCTCCGGTGGTTG+GGG 0.477662 1_4:-51754670 None:intergenic
GTTCCATCAGGTTGACCACC+AGG 0.484332 1_4:-51754088 None:intergenic
CTAATAATGCAAGATCAGAT+TGG 0.485011 1_4:-51755017 None:intergenic
TCGAACACTCTCGTGCAATC+AGG 0.486677 1_4:+51752631 Msa0168810:five_prime_UTR
TCAACCTGATGGAACTCAGC+TGG 0.487041 1_4:+51754096 Msa0168810:CDS
CCAGCAGGCATCGGTCCTCT+TGG 0.488412 1_4:-51754511 None:intergenic
TTGATACTGATTTCCCCAAA+AGG 0.488519 1_4:-51754188 None:intergenic
TTGAGTGGTGATGCTTGCCC+TGG 0.495063 1_4:-51752665 None:intergenic
CCAAGAGGACCGATGCCTGC+TGG 0.496911 1_4:+51754511 Msa0168810:CDS
TGTCCCAGCTGAGTTCCATC+AGG 0.498006 1_4:-51754100 None:intergenic
TCTTGGTTGACATTGTGCCT+AGG 0.499762 1_4:+51754458 Msa0168810:CDS
CCTCAAAATGCACAGCAAGC+TGG 0.503997 1_4:+51754571 Msa0168810:CDS
ATCACACTGAAGAGAACAAA+AGG 0.504661 1_4:+51754377 Msa0168810:CDS
TACCAGCCAAACCCAATGAC+TGG 0.504830 1_4:+51754025 Msa0168810:CDS
GAATCATCATAAACCATACT+TGG 0.506611 1_4:-51754919 None:intergenic
GACTGTGGAGCATACATGTT+GGG 0.509025 1_4:-51754619 None:intergenic
GGCGTGTGAAATGTTCATAT+TGG 0.517805 1_4:+51754333 Msa0168810:CDS
CATTCCGAAACGTGCTTCTG+TGG 0.523815 1_4:+51752597 Msa0168810:five_prime_UTR
CCATCAGCAGGTCCTCCAGC+AGG 0.525310 1_4:-51754526 None:intergenic
TGGTATGGATTAGAACCGTA+TGG 0.527698 1_4:-51754007 None:intergenic
TCTTGATCCTTGCCAAGGGA+AGG 0.527734 1_4:-51754246 None:intergenic
AATATGGGTGTTGTTGGTAG+TGG 0.529665 1_4:+51753974 Msa0168810:CDS
TCATCTTTCAAGTCCTCCCT+AGG 0.530171 1_4:-51754475 None:intergenic
CATGGCCAAAACCCAAATAT+GGG 0.534350 1_4:+51753959 Msa0168810:CDS
ATGGTTAGTAAGGTGAACTT+TGG 0.536359 1_4:+51754732 Msa0168810:three_prime_UTR
GGATTATGCATCATGAGTCC+AGG 0.538672 1_4:-51754598 None:intergenic
CAACCTGATGGAACTCAGCT+GGG 0.543463 1_4:+51754097 Msa0168810:CDS
CAATAAGGAATTCCATCAGC+AGG 0.543484 1_4:-51754538 None:intergenic
GGCGATTGTTGTTGCTCCGG+TGG 0.545817 1_4:-51754676 None:intergenic
AATCTTCTTGATCCTTGCCA+AGG 0.551207 1_4:-51754251 None:intergenic
GATGATATCAGCTGAGGCGC+CGG 0.551962 1_4:+51754297 Msa0168810:CDS
CCTGCTGGAGGACCTGCTGA+TGG 0.553104 1_4:+51754526 Msa0168810:CDS
CAACCTGGTGGTCAACCTGA+TGG 0.553542 1_4:+51754085 Msa0168810:CDS
GAAGGCGATTGTTGTTGCTC+CGG 0.554014 1_4:-51754679 None:intergenic
ATAAGAATCTAATGATCTGA+AGG 0.560767 1_4:-51754697 None:intergenic
GACATTGCAGCAGCAATCAC+AGG 0.562745 1_4:+51754418 Msa0168810:CDS
GAAGATTATGAAGGCTGATG+AGG 0.566353 1_4:+51754267 Msa0168810:CDS
ACCCAATGACTGGGTCACCT+GGG 0.574510 1_4:+51754035 Msa0168810:CDS
GCAGGTCCTCCAGCAGGCAT+CGG 0.575223 1_4:-51754520 None:intergenic
GGTATGGATTAGAACCGTAT+GGG 0.576207 1_4:-51754006 None:intergenic
GGTTGACATTGTGCCTAGGG+AGG 0.582771 1_4:+51754462 Msa0168810:CDS
ATCTTCTTGATCCTTGCCAA+GGG 0.584713 1_4:-51754250 None:intergenic
GTATTGTGATATTGCAGTAG+TGG 0.585313 1_4:+51754992 Msa0168810:three_prime_UTR
TCCACAGTCTCATCCATACA+TGG 0.588194 1_4:+51754633 Msa0168810:CDS
TCCTTGCCAAGGGAAGGCTG+TGG 0.591000 1_4:-51754240 None:intergenic
ACCACAGCCTTCCCTTGGCA+AGG 0.592871 1_4:+51754239 Msa0168810:CDS
GGCGCCGGTTGTATTTGCAA+GGG 0.593275 1_4:+51754312 Msa0168810:CDS
ACAGAGGGAACAACCATCCC+AGG 0.593577 1_4:-51754052 None:intergenic
CGTTTCGGAATGTTCTCGTA+CGG 0.595038 1_4:-51752586 None:intergenic
AAGGATCAAGAAGATTATGA+AGG 0.595150 1_4:+51754258 Msa0168810:CDS
AGTGGACAAATGGCCCCATA+CGG 0.596575 1_4:+51753992 Msa0168810:CDS
AGAATGGATCATCAAGGTCA+TGG 0.604516 1_4:+51753941 Msa0168810:intron
AATGACTGGGTCACCTGGGA+TGG 0.607597 1_4:+51754039 Msa0168810:CDS
TGAAACAGAATGGATCATCA+AGG 0.611963 1_4:+51753935 Msa0168810:intron
TCTGTTGGGACTATTCAACC+TGG 0.614722 1_4:+51754070 Msa0168810:CDS
CATACATGGCACCTCAAATG+TGG 0.616231 1_4:+51754647 Msa0168810:CDS
AGTTGGTACCTTGTGTTGAG+TGG 0.622959 1_4:-51752680 None:intergenic
ACCAGCCAAACCCAATGACT+GGG 0.624633 1_4:+51754026 Msa0168810:CDS
AGAGGACCGATGCCTGCTGG+AGG 0.625695 1_4:+51754514 Msa0168810:CDS
AAGCATCACCACTCAACACA+AGG 0.628345 1_4:+51752672 Msa0168810:five_prime_UTR
TCAGTATCAAGAAATTGAGA+AGG 0.629465 1_4:+51754201 Msa0168810:CDS
AGTGTGATTCCAAGAACGCA+AGG 0.629484 1_4:-51754362 None:intergenic
TTGAGAACACACTGAACATG+AGG 0.648120 1_4:+51755105 Msa0168810:three_prime_UTR
GGTTGAATAGTCCCAACAGA+GGG 0.660370 1_4:-51754067 None:intergenic
TGTGAAGATGATATCAGCTG+AGG 0.675937 1_4:+51754291 Msa0168810:CDS
GAAGTGCTTGCATTACCAAG+AGG 0.676504 1_4:+51754496 Msa0168810:CDS
GTTGGGACTATTCAACCTGG+TGG 0.686021 1_4:+51754073 Msa0168810:CDS
AGACTGTGGAGCATACATGT+TGG 0.689302 1_4:-51754620 None:intergenic
GTATGGATTAGAACCGTATG+GGG 0.698616 1_4:-51754005 None:intergenic
GCCATGTATGGATGAGACTG+TGG 0.701999 1_4:-51754634 None:intergenic
AGGTTGAATAGTCCCAACAG+AGG 0.742829 1_4:-51754068 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGGATTTTTTTTTTTTTTA+GGG + chr1_4:51753120-51753139 Msa0168810:intron 10.0%
!!! AAATGTTATATGATATTTTA+CGG + chr1_4:51753345-51753364 Msa0168810:intron 10.0%
!!! CTAGGATTTTTTTTTTTTTT+AGG + chr1_4:51753119-51753138 Msa0168810:intron 15.0%
!!! ATTTTTTTTTTTTTTAGGGT+TGG + chr1_4:51753124-51753143 Msa0168810:intron 15.0%
!! CAGTATATATTATAACTATA+TGG - chr1_4:51753150-51753169 None:intergenic 15.0%
!! TAATATATACTGTAAATTGT+TGG + chr1_4:51753158-51753177 Msa0168810:intron 15.0%
!! GTAAAATATCATATAACATT+TGG - chr1_4:51753346-51753365 None:intergenic 15.0%
!!! TTTAGGAGAAAAATTAATTT+TGG + chr1_4:51753475-51753494 Msa0168810:intron 15.0%
!!! TTAGGAGAAAAATTAATTTT+GGG + chr1_4:51753476-51753495 Msa0168810:intron 15.0%
!! AATCGAATTACATAATGAAA+AGG - chr1_4:51752728-51752747 None:intergenic 20.0%
!!! TTGTTTAGTTTTTCTTGTTA+CGG + chr1_4:51752746-51752765 Msa0168810:intron 20.0%
!! AGCAAATATCTAATATTTCA+TGG - chr1_4:51752828-51752847 None:intergenic 20.0%
!! TGAAATATTAGATATTTGCT+TGG + chr1_4:51752828-51752847 Msa0168810:intron 20.0%
!! AGCAGATTTATTTATGAATA+TGG + chr1_4:51752880-51752899 Msa0168810:intron 20.0%
!!! TATTTCTTTTATTCCATTCA+AGG + chr1_4:51753058-51753077 Msa0168810:intron 20.0%
!! AGATCATTAGATTCTTATTA+TGG + chr1_4:51754702-51754721 Msa0168810:exon 20.0%
!!! TGCAATAAATTAGTTTTACA+AGG + chr1_4:51754755-51754774 Msa0168810:three_prime_UTR 20.0%
!!! GCAATAAATTAGTTTTACAA+GGG + chr1_4:51754756-51754775 Msa0168810:three_prime_UTR 20.0%
!!! TTAAGTTTTACTATGATGTA+TGG - chr1_4:51754820-51754839 None:intergenic 20.0%
! AATATTAGATATTTGCTTGG+TGG + chr1_4:51752831-51752850 Msa0168810:intron 25.0%
! TTATGAATATGGTGAGAATT+AGG + chr1_4:51752891-51752910 Msa0168810:intron 25.0%
! TATGAATATGGTGAGAATTA+GGG + chr1_4:51752892-51752911 Msa0168810:intron 25.0%
! TTCCAAATACATGATTGATT+TGG + chr1_4:51753030-51753049 Msa0168810:intron 25.0%
! AAACACAAAAAAACCTTGAA+TGG - chr1_4:51753074-51753093 None:intergenic 25.0%
!!! AGGTTTTTTTGTGTTTTTCA+TGG + chr1_4:51753078-51753097 Msa0168810:intron 25.0%
! ATATACTGTAAATTGTTGGT+TGG + chr1_4:51753162-51753181 Msa0168810:intron 25.0%
! ATGTTTAATATGTTGCAAAG+AGG + chr1_4:51753269-51753288 Msa0168810:intron 25.0%
! TTTAATGCCAATAGCTATTT+AGG + chr1_4:51753458-51753477 Msa0168810:intron 25.0%
!! TTTTTCTCCTAAATAGCTAT+TGG - chr1_4:51753468-51753487 None:intergenic 25.0%
!!! GAGAAAAATTAATTTTGGGT+AGG + chr1_4:51753480-51753499 Msa0168810:intron 25.0%
! ATAACTTTGTACAAGTGTAT+TGG + chr1_4:51753592-51753611 Msa0168810:intron 25.0%
!! AATTAAGATTTGTTCGAGTT+TGG + chr1_4:51753619-51753638 Msa0168810:intron 25.0%
! ACTTTAGTACAAAGTTATGT+CGG - chr1_4:51753738-51753757 None:intergenic 25.0%
! AACTTTGTACTAAAGTGTAT+TGG + chr1_4:51753742-51753761 Msa0168810:intron 25.0%
!!! ATGTGCATATTGTTTTGTTT+TGG + chr1_4:51753872-51753891 Msa0168810:intron 25.0%
!! AACTGATATCTTTGATTTCT+TGG + chr1_4:51754441-51754460 Msa0168810:CDS 25.0%
! ATAAGAATCTAATGATCTGA+AGG - chr1_4:51754700-51754719 None:intergenic 25.0%
!!! TTCTTATTATGGAAGTGTTA+TGG + chr1_4:51754713-51754732 Msa0168810:three_prime_UTR 25.0%
! TTTACTATGATGTATGGAAA+TGG - chr1_4:51754814-51754833 None:intergenic 25.0%
!!! TTCAATGTCATTTTTCCTAA+TGG + chr1_4:51754857-51754876 Msa0168810:three_prime_UTR 25.0%
!! TAATAATTTTGCATGTTGTG+TGG + chr1_4:51755048-51755067 Msa0168810:three_prime_UTR 25.0%
! GTTATATTAGGTGTCTAATA+TGG + chr1_4:51755082-51755101 Msa0168810:three_prime_UTR 25.0%
!!! AATCAGGTTATTGATTTTCC+AGG + chr1_4:51752647-51752666 Msa0168810:five_prime_UTR 30.0%
!!! ATCAGGTTATTGATTTTCCA+GGG + chr1_4:51752648-51752667 Msa0168810:five_prime_UTR 30.0%
! CTTGTTATGCTTGATGTATA+TGG + chr1_4:51752958-51752977 Msa0168810:intron 30.0%
CTCCAAATCAATCATGTATT+TGG - chr1_4:51753035-51753054 None:intergenic 30.0%
!! CAAATACATGATTGATTTGG+AGG + chr1_4:51753033-51753052 Msa0168810:intron 30.0%
! TAAAGATTTTGTTCAAGTCC+TGG + chr1_4:51753770-51753789 Msa0168810:intron 30.0%
ACAATTACACGTGAACAAAA+GGG - chr1_4:51753846-51753865 None:intergenic 30.0%
AACAATTACACGTGAACAAA+AGG - chr1_4:51753847-51753866 None:intergenic 30.0%
TGATTTCACTTGAAACAGAA+TGG + chr1_4:51753925-51753944 Msa0168810:intron 30.0%
TCAGTATCAAGAAATTGAGA+AGG + chr1_4:51754201-51754220 Msa0168810:CDS 30.0%
AAGGATCAAGAAGATTATGA+AGG + chr1_4:51754258-51754277 Msa0168810:CDS 30.0%
! TGCAATGTCATTCTTTTGAA+GGG - chr1_4:51754407-51754426 None:intergenic 30.0%
CAGTAACATTAAGTTCCATT+AGG - chr1_4:51754875-51754894 None:intergenic 30.0%
TACAGATGAATAACCAAGTA+TGG + chr1_4:51754906-51754925 Msa0168810:three_prime_UTR 30.0%
GAATCATCATAAACCATACT+TGG - chr1_4:51754922-51754941 None:intergenic 30.0%
CTAATAATGCAAGATCAGAT+TGG - chr1_4:51755020-51755039 None:intergenic 30.0%
!! CACTAAATTCTGGGGTTAAT+CGG - chr1_4:51752798-51752817 None:intergenic 35.0%
!! TTTGCTTGGTGGTTTTTATG+CGG + chr1_4:51752842-51752861 Msa0168810:intron 35.0%
!! AAATATACACAAGTGCTGGA+TGG - chr1_4:51753011-51753030 None:intergenic 35.0%
TGGAAAATATACACAAGTGC+TGG - chr1_4:51753015-51753034 None:intergenic 35.0%
! TTTTGTGTTTTTCATGGTGC+TGG + chr1_4:51753084-51753103 Msa0168810:intron 35.0%
! TTTGTGTTTTTCATGGTGCT+GGG + chr1_4:51753085-51753104 Msa0168810:intron 35.0%
!! TATAACATTTGGTAGCGTAG+TGG - chr1_4:51753335-51753354 None:intergenic 35.0%
!! AATTAATTTTGGGTAGGCCA+TGG + chr1_4:51753486-51753505 Msa0168810:intron 35.0%
AAACAATGTTTATCGCGTAG+CGG - chr1_4:51753569-51753588 None:intergenic 35.0%
CAATTACACGTGAACAAAAG+GGG - chr1_4:51753845-51753864 None:intergenic 35.0%
TGAAACAGAATGGATCATCA+AGG + chr1_4:51753935-51753954 Msa0168810:intron 35.0%
TACCAACAACACCCATATTT+GGG - chr1_4:51753973-51753992 None:intergenic 35.0%
!! TTGATACTGATTTCCCCAAA+AGG - chr1_4:51754191-51754210 None:intergenic 35.0%
ATCACACTGAAGAGAACAAA+AGG + chr1_4:51754377-51754396 Msa0168810:CDS 35.0%
! CTGCAATGTCATTCTTTTGA+AGG - chr1_4:51754408-51754427 None:intergenic 35.0%
!! TGGAAGTGTTATGGTTAGTA+AGG + chr1_4:51754722-51754741 Msa0168810:three_prime_UTR 35.0%
ATGGTTAGTAAGGTGAACTT+TGG + chr1_4:51754732-51754751 Msa0168810:three_prime_UTR 35.0%
! CACAAGATGTTTCTAAGTAC+AGG + chr1_4:51754954-51754973 Msa0168810:three_prime_UTR 35.0%
GTATTGTGATATTGCAGTAG+TGG + chr1_4:51754992-51755011 Msa0168810:three_prime_UTR 35.0%
! GAAGCATGTGCTGTTATATT+AGG + chr1_4:51755070-51755089 Msa0168810:three_prime_UTR 35.0%
ACCCCAGAATTTAGTGTTTG+TGG + chr1_4:51752802-51752821 Msa0168810:intron 40.0%
GCCACAAACACTAAATTCTG+GGG - chr1_4:51752806-51752825 None:intergenic 40.0%
GGCCACAAACACTAAATTCT+GGG - chr1_4:51752807-51752826 None:intergenic 40.0%
TGGCCACAAACACTAAATTC+TGG - chr1_4:51752808-51752827 None:intergenic 40.0%
!! GTTGGTTGGTAAGACTTGAT+TGG + chr1_4:51753176-51753195 Msa0168810:intron 40.0%
TGGTATAATTGTCGAGACGA+AGG + chr1_4:51753196-51753215 Msa0168810:intron 40.0%
AACAATGTTTATCGCGTAGC+GGG - chr1_4:51753568-51753587 None:intergenic 40.0%
! TGTCGGTGTTTATCATGTAG+TGG - chr1_4:51753721-51753740 None:intergenic 40.0%
CTAGAATGCTATAGCATACC+AGG - chr1_4:51753791-51753810 None:intergenic 40.0%
TCAATTCCTGCACTTCACAT+GGG - chr1_4:51753899-51753918 None:intergenic 40.0%
ATCAATTCCTGCACTTCACA+TGG - chr1_4:51753900-51753919 None:intergenic 40.0%
AGAATGGATCATCAAGGTCA+TGG + chr1_4:51753941-51753960 Msa0168810:intron 40.0%
TCATGGCCAAAACCCAAATA+TGG + chr1_4:51753958-51753977 Msa0168810:CDS 40.0%
CATGGCCAAAACCCAAATAT+GGG + chr1_4:51753959-51753978 Msa0168810:CDS 40.0%
!! CAACACCCATATTTGGGTTT+TGG - chr1_4:51753967-51753986 None:intergenic 40.0%
! AACCCAAATATGGGTGTTGT+TGG + chr1_4:51753968-51753987 Msa0168810:CDS 40.0%
CTACCAACAACACCCATATT+TGG - chr1_4:51753974-51753993 None:intergenic 40.0%
!! AATATGGGTGTTGTTGGTAG+TGG + chr1_4:51753974-51753993 Msa0168810:CDS 40.0%
!! TGTTGTTGGTAGTGGACAAA+TGG + chr1_4:51753982-51754001 Msa0168810:CDS 40.0%
!! GTATGGATTAGAACCGTATG+GGG - chr1_4:51754008-51754027 None:intergenic 40.0%
!! GGTATGGATTAGAACCGTAT+GGG - chr1_4:51754009-51754028 None:intergenic 40.0%
!! TGGTATGGATTAGAACCGTA+TGG - chr1_4:51754010-51754029 None:intergenic 40.0%
! AACAACAACTCCAGACCTTT+TGG + chr1_4:51754173-51754192 Msa0168810:CDS 40.0%
! ACAACAACTCCAGACCTTTT+GGG + chr1_4:51754174-51754193 Msa0168810:CDS 40.0%
ATCTTCTTGATCCTTGCCAA+GGG - chr1_4:51754253-51754272 None:intergenic 40.0%
AATCTTCTTGATCCTTGCCA+AGG - chr1_4:51754254-51754273 None:intergenic 40.0%
GAAGATTATGAAGGCTGATG+AGG + chr1_4:51754267-51754286 Msa0168810:CDS 40.0%
TGTGAAGATGATATCAGCTG+AGG + chr1_4:51754291-51754310 Msa0168810:CDS 40.0%
GGCGTGTGAAATGTTCATAT+TGG + chr1_4:51754333-51754352 Msa0168810:CDS 40.0%
CAATAAGGAATTCCATCAGC+AGG - chr1_4:51754541-51754560 None:intergenic 40.0%
TTGAGAACACACTGAACATG+AGG + chr1_4:51755105-51755124 Msa0168810:three_prime_UTR 40.0%
CGTTTCGGAATGTTCTCGTA+CGG - chr1_4:51752589-51752608 None:intergenic 45.0%
AAGCATCACCACTCAACACA+AGG + chr1_4:51752672-51752691 Msa0168810:five_prime_UTR 45.0%
! AGTTGGTACCTTGTGTTGAG+TGG - chr1_4:51752683-51752702 None:intergenic 45.0%
AGTACGCGAATTGCAAGAGT+TGG - chr1_4:51752700-51752719 None:intergenic 45.0%
!! TGTTTTTCATGGTGCTGGGA+AGG + chr1_4:51753089-51753108 Msa0168810:intron 45.0%
!! GTTTTTCATGGTGCTGGGAA+GGG + chr1_4:51753090-51753109 Msa0168810:intron 45.0%
!! TTTTTCATGGTGCTGGGAAG+GGG + chr1_4:51753091-51753110 Msa0168810:intron 45.0%
ACAATGTTTATCGCGTAGCG+GGG - chr1_4:51753567-51753586 None:intergenic 45.0%
TCCACAATTGACACCATTGG+CGG - chr1_4:51753671-51753690 None:intergenic 45.0%
CAGTCCACAATTGACACCAT+TGG - chr1_4:51753674-51753693 None:intergenic 45.0%
! ATGGTGTCAATTGTGGACTG+CGG + chr1_4:51753674-51753693 Msa0168810:intron 45.0%
CAATTCCTGCACTTCACATG+GGG - chr1_4:51753898-51753917 None:intergenic 45.0%
GGTTGAATAGTCCCAACAGA+GGG - chr1_4:51754070-51754089 None:intergenic 45.0%
AGGTTGAATAGTCCCAACAG+AGG - chr1_4:51754071-51754090 None:intergenic 45.0%
TCTGTTGGGACTATTCAACC+TGG + chr1_4:51754070-51754089 Msa0168810:CDS 45.0%
! CAACAACTCCAGACCTTTTG+GGG + chr1_4:51754175-51754194 Msa0168810:CDS 45.0%
! ACTGATTTCCCCAAAAGGTC+TGG - chr1_4:51754186-51754205 None:intergenic 45.0%
TGGAGTTAACCTTGCGTTCT+TGG + chr1_4:51754353-51754372 Msa0168810:CDS 45.0%
AGTGTGATTCCAAGAACGCA+AGG - chr1_4:51754365-51754384 None:intergenic 45.0%
TCTTGGTTGACATTGTGCCT+AGG + chr1_4:51754458-51754477 Msa0168810:CDS 45.0%
CTTGGTTGACATTGTGCCTA+GGG + chr1_4:51754459-51754478 Msa0168810:CDS 45.0%
! TCATCTTTCAAGTCCTCCCT+AGG - chr1_4:51754478-51754497 None:intergenic 45.0%
! GAAGTGCTTGCATTACCAAG+AGG + chr1_4:51754496-51754515 Msa0168810:CDS 45.0%
TGAGGCGGCATATAGCAATA+AGG - chr1_4:51754556-51754575 None:intergenic 45.0%
GGATTATGCATCATGAGTCC+AGG - chr1_4:51754601-51754620 None:intergenic 45.0%
GACTGTGGAGCATACATGTT+GGG - chr1_4:51754622-51754641 None:intergenic 45.0%
AGACTGTGGAGCATACATGT+TGG - chr1_4:51754623-51754642 None:intergenic 45.0%
TCCACAGTCTCATCCATACA+TGG + chr1_4:51754633-51754652 Msa0168810:CDS 45.0%
! CACATTTGAGGTGCCATGTA+TGG - chr1_4:51754649-51754668 None:intergenic 45.0%
CATACATGGCACCTCAAATG+TGG + chr1_4:51754647-51754666 Msa0168810:CDS 45.0%
CATTCCGAAACGTGCTTCTG+TGG + chr1_4:51752597-51752616 Msa0168810:five_prime_UTR 50.0%
! AACGCCACAGAAGCACGTTT+CGG - chr1_4:51752604-51752623 None:intergenic 50.0%
TCGAACACTCTCGTGCAATC+AGG + chr1_4:51752631-51752650 Msa0168810:five_prime_UTR 50.0%
ATCCGCAAACGACACCAGTA+GGG - chr1_4:51753520-51753539 None:intergenic 50.0%
AATCCGCAAACGACACCAGT+AGG - chr1_4:51753521-51753540 None:intergenic 50.0%
TCAAATCACAGCTCCGCCAA+TGG + chr1_4:51753655-51753674 Msa0168810:intron 50.0%
! TCCGCCAATGGTGTCAATTG+TGG + chr1_4:51753667-51753686 Msa0168810:intron 50.0%
AGTGGACAAATGGCCCCATA+CGG + chr1_4:51753992-51754011 Msa0168810:CDS 50.0%
GTCATTGGGTTTGGCTGGTA+TGG - chr1_4:51754025-51754044 None:intergenic 50.0%
TACCAGCCAAACCCAATGAC+TGG + chr1_4:51754025-51754044 Msa0168810:CDS 50.0%
ACCAGCCAAACCCAATGACT+GGG + chr1_4:51754026-51754045 Msa0168810:CDS 50.0%
GGATGGTTGTTCCCTCTGTT+GGG + chr1_4:51754056-51754075 Msa0168810:CDS 50.0%
GTTGGGACTATTCAACCTGG+TGG + chr1_4:51754073-51754092 Msa0168810:CDS 50.0%
TCAACCTGATGGAACTCAGC+TGG + chr1_4:51754096-51754115 Msa0168810:CDS 50.0%
CAACCTGATGGAACTCAGCT+GGG + chr1_4:51754097-51754116 Msa0168810:CDS 50.0%
CAAAAACCACAGCCTTCCCT+TGG + chr1_4:51754234-51754253 Msa0168810:CDS 50.0%
TCTTGATCCTTGCCAAGGGA+AGG - chr1_4:51754249-51754268 None:intergenic 50.0%
CACGCCCTTGCAAATACAAC+CGG - chr1_4:51754319-51754338 None:intergenic 50.0%
GACATTGCAGCAGCAATCAC+AGG + chr1_4:51754418-51754437 Msa0168810:CDS 50.0%
!!! GCTTGCTGTGCATTTTGAGG+CGG - chr1_4:51754571-51754590 None:intergenic 50.0%
!!! CCAGCTTGCTGTGCATTTTG+AGG - chr1_4:51754574-51754593 None:intergenic 50.0%
CCTCAAAATGCACAGCAAGC+TGG + chr1_4:51754571-51754590 Msa0168810:CDS 50.0%
GCCATGTATGGATGAGACTG+TGG - chr1_4:51754637-51754656 None:intergenic 50.0%
! TTGTTGTTGCTCCGGTGGTT+GGG - chr1_4:51754674-51754693 None:intergenic 50.0%
! ATTGTTGTTGCTCCGGTGGT+TGG - chr1_4:51754675-51754694 None:intergenic 50.0%
! GAAGGCGATTGTTGTTGCTC+CGG - chr1_4:51754682-51754701 None:intergenic 50.0%
! TTGAGTGGTGATGCTTGCCC+TGG - chr1_4:51752668-51752687 None:intergenic 55.0%
! TTTTGTCCCTCGTAGGTCGC+AGG - chr1_4:51753302-51753321 None:intergenic 55.0%
GACCTACGAGGGACAAAACG+CGG + chr1_4:51753304-51753323 Msa0168810:intron 55.0%
ACCTACGAGGGACAAAACGC+GGG + chr1_4:51753305-51753324 Msa0168810:intron 55.0%
TCCGCAAACGACACCAGTAG+GGG - chr1_4:51753519-51753538 None:intergenic 55.0%
!! CTGGTGTCGTTTGCGGATTG+CGG + chr1_4:51753522-51753541 Msa0168810:intron 55.0%
TTTGGCCCCATGTGAAGTGC+AGG + chr1_4:51753890-51753909 Msa0168810:intron 55.0%
ACCCAGTCATTGGGTTTGGC+TGG - chr1_4:51754030-51754049 None:intergenic 55.0%
GGTGACCCAGTCATTGGGTT+TGG - chr1_4:51754034-51754053 None:intergenic 55.0%
AACCCAATGACTGGGTCACC+TGG + chr1_4:51754034-51754053 Msa0168810:CDS 55.0%
ACCCAATGACTGGGTCACCT+GGG + chr1_4:51754035-51754054 Msa0168810:CDS 55.0%
TCCCAGGTGACCCAGTCATT+GGG - chr1_4:51754039-51754058 None:intergenic 55.0%
ATCCCAGGTGACCCAGTCAT+TGG - chr1_4:51754040-51754059 None:intergenic 55.0%
AATGACTGGGTCACCTGGGA+TGG + chr1_4:51754039-51754058 Msa0168810:CDS 55.0%
ACAGAGGGAACAACCATCCC+AGG - chr1_4:51754055-51754074 None:intergenic 55.0%
GGGATGGTTGTTCCCTCTGT+TGG + chr1_4:51754055-51754074 Msa0168810:CDS 55.0%
CAACCTGGTGGTCAACCTGA+TGG + chr1_4:51754085-51754104 Msa0168810:CDS 55.0%
!! GTTCCATCAGGTTGACCACC+AGG - chr1_4:51754091-51754110 None:intergenic 55.0%
TGTCCCAGCTGAGTTCCATC+AGG - chr1_4:51754103-51754122 None:intergenic 55.0%
GATGATATCAGCTGAGGCGC+CGG + chr1_4:51754297-51754316 Msa0168810:CDS 55.0%
!! AGGCGCCGGTTGTATTTGCA+AGG + chr1_4:51754311-51754330 Msa0168810:CDS 55.0%
!! GGCGCCGGTTGTATTTGCAA+GGG + chr1_4:51754312-51754331 Msa0168810:CDS 55.0%
GGTTGACATTGTGCCTAGGG+AGG + chr1_4:51754462-51754481 Msa0168810:CDS 55.0%
GCACAGCAAGCTGGAAATCC+TGG + chr1_4:51754580-51754599 Msa0168810:CDS 55.0%
TCAAATGTGGCCCCAACCAC+CGG + chr1_4:51754660-51754679 Msa0168810:CDS 55.0%
TGTTGTTGCTCCGGTGGTTG+GGG - chr1_4:51754673-51754692 None:intergenic 55.0%
TGCTGGGAAGGGGTGAAGCT+AGG + chr1_4:51753101-51753120 Msa0168810:intron 60.0%
ACTCGATCCTGCGACCTACG+AGG + chr1_4:51753292-51753311 Msa0168810:intron 60.0%
CTCGATCCTGCGACCTACGA+GGG + chr1_4:51753293-51753312 Msa0168810:intron 60.0%
! ACCCGCGTTTTGTCCCTCGT+AGG - chr1_4:51753309-51753328 None:intergenic 60.0%
CCGCAAACGACACCAGTAGG+GGG - chr1_4:51753518-51753537 None:intergenic 60.0%
!! CCCCCTACTGGTGTCGTTTG+CGG + chr1_4:51753515-51753534 Msa0168810:intron 60.0%
ACCACAGCCTTCCCTTGGCA+AGG + chr1_4:51754239-51754258 Msa0168810:CDS 60.0%
TCCTTGCCAAGGGAAGGCTG+TGG - chr1_4:51754243-51754262 None:intergenic 60.0%
GGTGGTTGGGGCCACATTTG+AGG - chr1_4:51754661-51754680 None:intergenic 60.0%
! GGCGATTGTTGTTGCTCCGG+TGG - chr1_4:51754679-51754698 None:intergenic 60.0%
CCAGTAGGGGGAGCTAGCCA+TGG - chr1_4:51753506-51753525 None:intergenic 65.0%
! CCATGGCTAGCTCCCCCTAC+TGG + chr1_4:51753503-51753522 Msa0168810:intron 65.0%
!! CCAGCAGGCATCGGTCCTCT+TGG - chr1_4:51754514-51754533 None:intergenic 65.0%
CCAAGAGGACCGATGCCTGC+TGG + chr1_4:51754511-51754530 Msa0168810:CDS 65.0%
AGAGGACCGATGCCTGCTGG+AGG + chr1_4:51754514-51754533 Msa0168810:CDS 65.0%
GCAGGTCCTCCAGCAGGCAT+CGG - chr1_4:51754523-51754542 None:intergenic 65.0%
CCATCAGCAGGTCCTCCAGC+AGG - chr1_4:51754529-51754548 None:intergenic 65.0%
CCTGCTGGAGGACCTGCTGA+TGG + chr1_4:51754526-51754545 Msa0168810:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 51752582 51755167 51752582 ID=Msa0168810;Name=Msa0168810
chr1_4 mRNA 51752582 51755167 51752582 ID=Msa0168810-mRNA-1;Parent=Msa0168810;Name=Msa0168810-mRNA-1;_AED=0.01;_eAED=0.01;_QI=112|0|0.5|1|0|0.5|2|456|255
chr1_4 exon 51752582 51752693 51752582 ID=Msa0168810-mRNA-1:exon:15249;Parent=Msa0168810-mRNA-1
chr1_4 exon 51753944 51755167 51753944 ID=Msa0168810-mRNA-1:exon:15250;Parent=Msa0168810-mRNA-1
chr1_4 five_prime_UTR 51752582 51752693 51752582 ID=Msa0168810-mRNA-1:five_prime_utr;Parent=Msa0168810-mRNA-1
chr1_4 CDS 51753944 51754711 51753944 ID=Msa0168810-mRNA-1:cds;Parent=Msa0168810-mRNA-1
chr1_4 three_prime_UTR 51754712 51755167 51754712 ID=Msa0168810-mRNA-1:three_prime_utr;Parent=Msa0168810-mRNA-1
Gene Sequence

>Msa0168810

ATGGATCATCAAGGTCATGGCCAAAACCCAAATATGGGTGTTGTTGGTAGTGGACAAATGGCCCCATACGGTTCTAATCCATACCAGCCAAACCCAATGACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGACTATTCAACCTGGTGGTCAACCTGATGGAACTCAGCTGGGACAACATCAGCTTGCTTATCAGCACATTCATCAGCAGCAACAGCAGCAGCAACAACAACAACTCCAGACCTTTTGGGGAAATCAGTATCAAGAAATTGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCTGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGTGAAATGTTCATATTGGAGTTAACCTTGCGTTCTTGGAATCACACTGAAGAGAACAAAAGGCGAACCCTTCAAAAGAATGACATTGCAGCAGCAATCACAGGAACTGATATCTTTGATTTCTTGGTTGACATTGTGCCTAGGGAGGACTTGAAAGATGAAGTGCTTGCATTACCAAGAGGACCGATGCCTGCTGGAGGACCTGCTGATGGAATTCCTTATTGCTATATGCCGCCTCAAAATGCACAGCAAGCTGGAAATCCTGGACTCATGATGCATAATCCCAACATGTATGCTCCACAGTCTCATCCATACATGGCACCTCAAATGTGGCCCCAACCACCGGAGCAACAACAATCGCCTTCAGATCATTAG
Protein sequence

>Msa0168810

MDHQGHGQNPNMGVVGSGQMAPYGSNPYQPNPMTGSPGMVVPSVGTIQPGGQPDGTQLGQHQLAYQHIHQQQQQQQQQQLQTFWGNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVKMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITGTDIFDFLVDIVPREDLKDEVLALPRGPMPAGGPADGIPYCYMPPQNAQQAGNPGLMMHNPNMYAPQSHPYMAPQMWPQPPEQQQSPSDH*