AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0033270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033270 MtrunA17_Chr1g0191681 98.828 256 2 1 1 255 1 256 0.0 518
Msa0033270 MtrunA17_Chr7g0272991 81.154 260 44 2 1 255 1 260 8.29e-145 405
Msa0033270 MtrunA17_Chr2g0310741 58.065 217 75 8 16 226 20 226 1.33e-69 215
Msa0033270 MtrunA17_Chr3g0132191 82.524 103 18 0 83 185 37 139 1.84e-58 185
Msa0033270 MtrunA17_Chr2g0288481 50.000 184 70 7 82 254 66 238 2.27e-53 172
Msa0033270 MtrunA17_Chr2g0318881 59.341 91 36 1 100 189 21 111 8.17e-33 116
Msa0033270 MtrunA17_Chr2g0318921 63.855 83 29 1 100 181 21 103 3.29e-32 114
Msa0033270 MtrunA17_Chr8g0376351 61.333 75 27 1 119 191 1 75 2.48e-27 100
Msa0033270 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 4.26e-12 64.7
Msa0033270 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.08e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033270 AT1G08970.3 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033270 AT1G08970.4 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033270 AT1G08970.2 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033270 AT1G08970.1 64.344 244 60 9 1 242 1 219 1.73e-86 257
Msa0033270 AT1G54830.2 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033270 AT1G54830.3 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033270 AT1G54830.1 64.655 232 56 4 23 254 12 217 2.81e-86 256
Msa0033270 AT1G56170.1 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0033270 AT1G56170.2 67.785 149 34 3 80 226 55 191 1.76e-66 205
Msa0033270 AT3G48590.1 69.863 146 41 3 83 228 47 189 3.11e-64 201
Msa0033270 AT5G63470.2 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0033270 AT5G63470.1 68.456 149 41 3 83 228 60 205 1.71e-61 194
Msa0033270 AT5G50480.1 48.276 174 55 7 79 243 35 182 6.37e-44 147
Msa0033270 AT5G27910.1 47.794 136 61 3 78 206 16 148 1.15e-35 125
Msa0033270 AT5G50490.1 39.106 179 90 6 71 244 9 173 3.10e-32 117
Msa0033270 AT5G50470.1 48.148 108 47 2 79 180 44 148 1.20e-28 108
Msa0033270 AT5G38140.1 45.000 100 51 2 80 178 48 144 5.12e-26 101

Find 109 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATATCTTTGATTTCT+TGG 0.152930 1_1:+60720885 Msa0033270:CDS
CTACCAACAACACCCATATT+TGG 0.174332 1_1:-60720415 None:intergenic
TGGAGTTAACCTTGCGTTCT+TGG 0.214555 1_1:+60720797 Msa0033270:CDS
AGATCATTAGATTCTTATTA+TGG 0.246047 1_1:+60721146 Msa0033270:exon
TCATGGCCAAAACCCAAATA+TGG 0.254115 1_1:+60720402 Msa0033270:CDS
AACAACAACTCCAGACCTTT+TGG 0.267438 1_1:+60720617 Msa0033270:CDS
TACAGATGAATAACCAAGTA+TGG 0.276951 1_1:+60721350 Msa0033270:three_prime_UTR
CAACACCCATATTTGGGTTT+TGG 0.289322 1_1:-60720408 None:intergenic
GTCATTGGGTTTGGCTGGTA+TGG 0.344732 1_1:-60720466 None:intergenic
TACCAACAACACCCATATTT+GGG 0.353196 1_1:-60720414 None:intergenic
GTTATATTAGGTGTCTAATA+TGG 0.356708 1_1:+60721526 Msa0033270:three_prime_UTR
TTCTTATTATGGAAGTGTTA+TGG 0.362115 1_1:+60721157 Msa0033270:three_prime_UTR
ATTGTTGTTGCTCCGGTGGT+TGG 0.362576 1_1:-60721116 None:intergenic
TTGTTGTTGCTCCGGTGGTT+GGG 0.366842 1_1:-60721115 None:intergenic
GAAGCATGTGCTGTTATATT+AGG 0.367404 1_1:+60721514 Msa0033270:three_prime_UTR
GGTGACCCAGTCATTGGGTT+TGG 0.375542 1_1:-60720475 None:intergenic
GGGATGGTTGTTCCCTCTGT+TGG 0.377579 1_1:+60720499 Msa0033270:CDS
CAGTAACATTAAGTTCCATT+AGG 0.385447 1_1:-60721316 None:intergenic
GCACAGCAAGCTGGAAATCC+TGG 0.386872 1_1:+60721024 Msa0033270:CDS
GGATGGTTGTTCCCTCTGTT+GGG 0.395560 1_1:+60720500 Msa0033270:CDS
ATCCCAGGTGACCCAGTCAT+TGG 0.407383 1_1:-60720481 None:intergenic
TGAGGCGGCATATAGCAATA+AGG 0.408347 1_1:-60720997 None:intergenic
ACCCAGTCATTGGGTTTGGC+TGG 0.417557 1_1:-60720471 None:intergenic
AACCCAATGACTGGGTCACC+TGG 0.422707 1_1:+60720478 Msa0033270:CDS
TGGAAGTGTTATGGTTAGTA+AGG 0.425704 1_1:+60721166 Msa0033270:three_prime_UTR
GGTGGTTGGGGCCACATTTG+AGG 0.430701 1_1:-60721102 None:intergenic
CACGCCCTTGCAAATACAAC+CGG 0.432250 1_1:-60720760 None:intergenic
TTTACTATGATGTATGGAAA+TGG 0.439756 1_1:-60721255 None:intergenic
CTTGGTTGACATTGTGCCTA+GGG 0.441263 1_1:+60720903 Msa0033270:CDS
AACCCAAATATGGGTGTTGT+TGG 0.445950 1_1:+60720412 Msa0033270:CDS
ACTGATTTCCCCAAAAGGTC+TGG 0.448617 1_1:-60720627 None:intergenic
TCCCAGGTGACCCAGTCATT+GGG 0.450412 1_1:-60720480 None:intergenic
CACAAGATGTTTCTAAGTAC+AGG 0.455147 1_1:+60721398 Msa0033270:three_prime_UTR
AACGCCACAGAAGCACGTTT+CGG 0.455724 1_1:-60719032 None:intergenic
CACATTTGAGGTGCCATGTA+TGG 0.456179 1_1:-60721090 None:intergenic
TCAAATGTGGCCCCAACCAC+CGG 0.464006 1_1:+60721104 Msa0033270:CDS
AGGCGCCGGTTGTATTTGCA+AGG 0.466746 1_1:+60720755 Msa0033270:CDS
TGTTGTTGGTAGTGGACAAA+TGG 0.476420 1_1:+60720426 Msa0033270:CDS
TGTTGTTGCTCCGGTGGTTG+GGG 0.477662 1_1:-60721114 None:intergenic
GTTCCATCAGGTTGACCACC+AGG 0.484332 1_1:-60720532 None:intergenic
CTAATAATGCAAGATCAGAT+TGG 0.485011 1_1:-60721461 None:intergenic
TCGAACACTCTCGTGCAATC+AGG 0.486677 1_1:+60719062 Msa0033270:five_prime_UTR
TCAACCTGATGGAACTCAGC+TGG 0.487041 1_1:+60720540 Msa0033270:CDS
CCAGCAGGCATCGGTCCTCT+TGG 0.488412 1_1:-60720955 None:intergenic
TTGATACTGATTTCCCCAAA+AGG 0.488519 1_1:-60720632 None:intergenic
TTGAGTGGTGATGCTTGCCC+TGG 0.495063 1_1:-60719096 None:intergenic
CCAAGAGGACCGATGCCTGC+TGG 0.496911 1_1:+60720955 Msa0033270:CDS
TGTCCCAGCTGAGTTCCATC+AGG 0.498006 1_1:-60720544 None:intergenic
TCTTGGTTGACATTGTGCCT+AGG 0.499762 1_1:+60720902 Msa0033270:CDS
CCTCAAAATGCACAGCAAGC+TGG 0.503997 1_1:+60721015 Msa0033270:CDS
ATCACACTGAAGAGAACAAA+AGG 0.504661 1_1:+60720821 Msa0033270:CDS
TACCAGCCAAACCCAATGAC+TGG 0.504830 1_1:+60720469 Msa0033270:CDS
GAATCATCATAAACCATACT+TGG 0.506611 1_1:-60721363 None:intergenic
GACTGTGGAGCATACATGTT+GGG 0.509025 1_1:-60721063 None:intergenic
GGCGTGTGAAATGTTCATAT+TGG 0.517805 1_1:+60720777 Msa0033270:CDS
CATTCCGAAACGTGCTTCTG+TGG 0.523815 1_1:+60719028 Msa0033270:five_prime_UTR
CCATCAGCAGGTCCTCCAGC+AGG 0.525310 1_1:-60720970 None:intergenic
TGGTATGGATTAGAACCGTA+TGG 0.527698 1_1:-60720451 None:intergenic
TCTTGATCCTTGCCAAGGGA+AGG 0.527734 1_1:-60720690 None:intergenic
AATATGGGTGTTGTTGGTAG+TGG 0.529665 1_1:+60720418 Msa0033270:CDS
TCATCTTTCAAGTCCTCCCT+AGG 0.530171 1_1:-60720919 None:intergenic
CATGGCCAAAACCCAAATAT+GGG 0.534350 1_1:+60720403 Msa0033270:CDS
ATGGTTAGTAAGGTGAACTT+TGG 0.536359 1_1:+60721176 Msa0033270:three_prime_UTR
GGATTATGCATCATGAGTCC+AGG 0.538672 1_1:-60721042 None:intergenic
CAACCTGATGGAACTCAGCT+GGG 0.543463 1_1:+60720541 Msa0033270:CDS
CAATAAGGAATTCCATCAGC+AGG 0.543484 1_1:-60720982 None:intergenic
GGCGATTGTTGTTGCTCCGG+TGG 0.545817 1_1:-60721120 None:intergenic
AATCTTCTTGATCCTTGCCA+AGG 0.551207 1_1:-60720695 None:intergenic
GATGATATCAGCTGAGGCGC+CGG 0.551962 1_1:+60720741 Msa0033270:CDS
CCTGCTGGAGGACCTGCTGA+TGG 0.553104 1_1:+60720970 Msa0033270:CDS
CAACCTGGTGGTCAACCTGA+TGG 0.553542 1_1:+60720529 Msa0033270:CDS
GAAGGCGATTGTTGTTGCTC+CGG 0.554014 1_1:-60721123 None:intergenic
ATAAGAATCTAATGATCTGA+AGG 0.560767 1_1:-60721141 None:intergenic
GACATTGCAGCAGCAATCAC+AGG 0.562745 1_1:+60720862 Msa0033270:CDS
GAAGATTATGAAGGCTGATG+AGG 0.566353 1_1:+60720711 Msa0033270:CDS
ACCCAATGACTGGGTCACCT+GGG 0.574510 1_1:+60720479 Msa0033270:CDS
GCAGGTCCTCCAGCAGGCAT+CGG 0.575223 1_1:-60720964 None:intergenic
GGTATGGATTAGAACCGTAT+GGG 0.576207 1_1:-60720450 None:intergenic
GGTTGACATTGTGCCTAGGG+AGG 0.582771 1_1:+60720906 Msa0033270:CDS
ATCTTCTTGATCCTTGCCAA+GGG 0.584713 1_1:-60720694 None:intergenic
GTATTGTGATATTGCAGTAG+TGG 0.585313 1_1:+60721436 Msa0033270:three_prime_UTR
TCCACAGTCTCATCCATACA+TGG 0.588194 1_1:+60721077 Msa0033270:CDS
TCCTTGCCAAGGGAAGGCTG+TGG 0.591000 1_1:-60720684 None:intergenic
ACCACAGCCTTCCCTTGGCA+AGG 0.592871 1_1:+60720683 Msa0033270:CDS
GGCGCCGGTTGTATTTGCAA+GGG 0.593275 1_1:+60720756 Msa0033270:CDS
ACAGAGGGAACAACCATCCC+AGG 0.593577 1_1:-60720496 None:intergenic
CGTTTCGGAATGTTCTCGTA+CGG 0.595038 1_1:-60719017 None:intergenic
AAGGATCAAGAAGATTATGA+AGG 0.595150 1_1:+60720702 Msa0033270:CDS
AGTGGACAAATGGCCCCATA+CGG 0.596575 1_1:+60720436 Msa0033270:CDS
AGAATGGATCATCAAGGTCA+TGG 0.604516 1_1:+60720385 Msa0033270:intron
AATGACTGGGTCACCTGGGA+TGG 0.607597 1_1:+60720483 Msa0033270:CDS
TGAAACAGAATGGATCATCA+AGG 0.611963 1_1:+60720379 Msa0033270:intron
TCTGTTGGGACTATTCAACC+TGG 0.614722 1_1:+60720514 Msa0033270:CDS
CATACATGGCACCTCAAATG+TGG 0.616231 1_1:+60721091 Msa0033270:CDS
AGTTGGTACCTTGTGTTGAG+TGG 0.622959 1_1:-60719111 None:intergenic
ACCAGCCAAACCCAATGACT+GGG 0.624633 1_1:+60720470 Msa0033270:CDS
AGAGGACCGATGCCTGCTGG+AGG 0.625695 1_1:+60720958 Msa0033270:CDS
AAGCATCACCACTCAACACA+AGG 0.628345 1_1:+60719103 Msa0033270:five_prime_UTR
TCAGTATCAAGAAATTGAGA+AGG 0.629465 1_1:+60720645 Msa0033270:CDS
AGTGTGATTCCAAGAACGCA+AGG 0.629484 1_1:-60720806 None:intergenic
TTGAGAACACACTGAACATG+AGG 0.648120 1_1:+60721549 Msa0033270:three_prime_UTR
GGTTGAATAGTCCCAACAGA+GGG 0.660370 1_1:-60720511 None:intergenic
TGTGAAGATGATATCAGCTG+AGG 0.675937 1_1:+60720735 Msa0033270:CDS
GAAGTGCTTGCATTACCAAG+AGG 0.676504 1_1:+60720940 Msa0033270:CDS
GTTGGGACTATTCAACCTGG+TGG 0.686021 1_1:+60720517 Msa0033270:CDS
AGACTGTGGAGCATACATGT+TGG 0.689302 1_1:-60721064 None:intergenic
GTATGGATTAGAACCGTATG+GGG 0.698616 1_1:-60720449 None:intergenic
GCCATGTATGGATGAGACTG+TGG 0.701999 1_1:-60721078 None:intergenic
AGGTTGAATAGTCCCAACAG+AGG 0.742829 1_1:-60720512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGGATTTTTTTTTTTTTTT+AGG + chr1_1:60719551-60719570 Msa0033270:intron 10.0%
!!! AGGATTTTTTTTTTTTTTTA+GGG + chr1_1:60719552-60719571 Msa0033270:intron 10.0%
!!! AAATGTTATATGATATTTTA+CGG + chr1_1:60719777-60719796 Msa0033270:intron 10.0%
!!! TTTTTTTTTTTTTTTAGGGT+TGG + chr1_1:60719556-60719575 Msa0033270:intron 15.0%
!! CAGTATATATTATAACTATA+TGG - chr1_1:60719582-60719601 None:intergenic 15.0%
!! TAATATATACTGTAAATTGT+TGG + chr1_1:60719590-60719609 Msa0033270:intron 15.0%
!! GTAAAATATCATATAACATT+TGG - chr1_1:60719778-60719797 None:intergenic 15.0%
!!! TTTAGGAGAAAAATTAATTT+TGG + chr1_1:60719919-60719938 Msa0033270:intron 15.0%
!!! TTAGGAGAAAAATTAATTTT+GGG + chr1_1:60719920-60719939 Msa0033270:intron 15.0%
!! AATCGAATTACATAATGAAA+AGG - chr1_1:60719159-60719178 None:intergenic 20.0%
!!! TTGTTTAGTTTTTCTTGTTA+CGG + chr1_1:60719177-60719196 Msa0033270:intron 20.0%
!! AGCAAATATCTAATATTTCA+TGG - chr1_1:60719259-60719278 None:intergenic 20.0%
!! TGAAATATTAGATATTTGCT+TGG + chr1_1:60719259-60719278 Msa0033270:intron 20.0%
!! AGCAGATTTATTTATGAATA+TGG + chr1_1:60719311-60719330 Msa0033270:intron 20.0%
!!! TATTTCTTTTATTCCATTCA+AGG + chr1_1:60719489-60719508 Msa0033270:intron 20.0%
!! AGATCATTAGATTCTTATTA+TGG + chr1_1:60721146-60721165 Msa0033270:exon 20.0%
!!! TGCAATAAATTAGTTTTACA+AGG + chr1_1:60721199-60721218 Msa0033270:three_prime_UTR 20.0%
!!! GCAATAAATTAGTTTTACAA+GGG + chr1_1:60721200-60721219 Msa0033270:three_prime_UTR 20.0%
!!! TTAAGTTTTACTATGATGTA+TGG - chr1_1:60721264-60721283 None:intergenic 20.0%
!!! TAATAATTTTGTATGTTGTG+TGG + chr1_1:60721492-60721511 Msa0033270:three_prime_UTR 20.0%
! AATATTAGATATTTGCTTGG+TGG + chr1_1:60719262-60719281 Msa0033270:intron 25.0%
! TTATGAATATGGTGAGAATT+AGG + chr1_1:60719322-60719341 Msa0033270:intron 25.0%
! TATGAATATGGTGAGAATTA+GGG + chr1_1:60719323-60719342 Msa0033270:intron 25.0%
! TTCCAAATACATGATTGATT+TGG + chr1_1:60719461-60719480 Msa0033270:intron 25.0%
! AAACACAAAAAAACCTTGAA+TGG - chr1_1:60719505-60719524 None:intergenic 25.0%
!!! AGGTTTTTTTGTGTTTTTCA+TGG + chr1_1:60719509-60719528 Msa0033270:intron 25.0%
! ATATACTGTAAATTGTTGGT+TGG + chr1_1:60719594-60719613 Msa0033270:intron 25.0%
! ATGTTTAATATGTTGCAAAG+AGG + chr1_1:60719701-60719720 Msa0033270:intron 25.0%
! TTTAATGCCAATAGCTATTT+AGG + chr1_1:60719902-60719921 Msa0033270:intron 25.0%
!! TTTTTCTCCTAAATAGCTAT+TGG - chr1_1:60719912-60719931 None:intergenic 25.0%
!!! GAGAAAAATTAATTTTGGGT+AGG + chr1_1:60719924-60719943 Msa0033270:intron 25.0%
! ATAACTTTGTACAAGTGTAT+TGG + chr1_1:60720036-60720055 Msa0033270:intron 25.0%
!! AATTAAGATTTGTTCGAGTT+TGG + chr1_1:60720063-60720082 Msa0033270:intron 25.0%
! ACTTTAGTACAAAGTTATGT+CGG - chr1_1:60720182-60720201 None:intergenic 25.0%
! AACTTTGTACTAAAGTGTAT+TGG + chr1_1:60720186-60720205 Msa0033270:intron 25.0%
!!! ATGTGCATATTGTTTTGTTT+TGG + chr1_1:60720316-60720335 Msa0033270:intron 25.0%
!! AACTGATATCTTTGATTTCT+TGG + chr1_1:60720885-60720904 Msa0033270:CDS 25.0%
! ATAAGAATCTAATGATCTGA+AGG - chr1_1:60721144-60721163 None:intergenic 25.0%
!!! TTCTTATTATGGAAGTGTTA+TGG + chr1_1:60721157-60721176 Msa0033270:three_prime_UTR 25.0%
! TTTACTATGATGTATGGAAA+TGG - chr1_1:60721258-60721277 None:intergenic 25.0%
!!! TTCAAAGTCATTTTTCCTAA+TGG + chr1_1:60721301-60721320 Msa0033270:three_prime_UTR 25.0%
! GTTATATTAGGTGTCTAATA+TGG + chr1_1:60721526-60721545 Msa0033270:three_prime_UTR 25.0%
!!! AATCAGGTTATTGATTTTCC+AGG + chr1_1:60719078-60719097 Msa0033270:five_prime_UTR 30.0%
!!! ATCAGGTTATTGATTTTCCA+GGG + chr1_1:60719079-60719098 Msa0033270:five_prime_UTR 30.0%
! CTTGTTATGCTTGATGTATA+TGG + chr1_1:60719389-60719408 Msa0033270:intron 30.0%
CTCCAAATCAATCATGTATT+TGG - chr1_1:60719466-60719485 None:intergenic 30.0%
!! CAAATACATGATTGATTTGG+AGG + chr1_1:60719464-60719483 Msa0033270:intron 30.0%
! TAAAGATTTTGTTCAAGTCC+TGG + chr1_1:60720214-60720233 Msa0033270:intron 30.0%
ACAATTACACGTGAACAAAA+GGG - chr1_1:60720290-60720309 None:intergenic 30.0%
AACAATTACACGTGAACAAA+AGG - chr1_1:60720291-60720310 None:intergenic 30.0%
TGATTTCACTTGAAACAGAA+TGG + chr1_1:60720369-60720388 Msa0033270:intron 30.0%
TCAGTATCAAGAAATTGAGA+AGG + chr1_1:60720645-60720664 Msa0033270:CDS 30.0%
AAGGATCAAGAAGATTATGA+AGG + chr1_1:60720702-60720721 Msa0033270:CDS 30.0%
! TGCAATGTCATTCTTTTGAA+GGG - chr1_1:60720851-60720870 None:intergenic 30.0%
CAGTAACATTAAGTTCCATT+AGG - chr1_1:60721319-60721338 None:intergenic 30.0%
TACAGATGAATAACCAAGTA+TGG + chr1_1:60721350-60721369 Msa0033270:three_prime_UTR 30.0%
GAATCATCATAAACCATACT+TGG - chr1_1:60721366-60721385 None:intergenic 30.0%
CTAATAATGCAAGATCAGAT+TGG - chr1_1:60721464-60721483 None:intergenic 30.0%
!! CACTAAATTCTGGGGTTAAT+CGG - chr1_1:60719229-60719248 None:intergenic 35.0%
!! TTTGCTTGGTGGTTTTTATG+CGG + chr1_1:60719273-60719292 Msa0033270:intron 35.0%
!! AAATATACACAAGTGCTGGA+TGG - chr1_1:60719442-60719461 None:intergenic 35.0%
TGGAAAATATACACAAGTGC+TGG - chr1_1:60719446-60719465 None:intergenic 35.0%
! TTTTGTGTTTTTCATGGTGC+TGG + chr1_1:60719515-60719534 Msa0033270:intron 35.0%
! TTTGTGTTTTTCATGGTGCT+GGG + chr1_1:60719516-60719535 Msa0033270:intron 35.0%
TGGTATAATTGTTGAGACGA+AGG + chr1_1:60719628-60719647 Msa0033270:intron 35.0%
!! TATAACATTTGGTAGCGTAG+TGG - chr1_1:60719767-60719786 None:intergenic 35.0%
!! AATTAATTTTGGGTAGGCCA+TGG + chr1_1:60719930-60719949 Msa0033270:intron 35.0%
AAACAATGTTTATCGCGTAG+CGG - chr1_1:60720013-60720032 None:intergenic 35.0%
CAATTACACGTGAACAAAAG+GGG - chr1_1:60720289-60720308 None:intergenic 35.0%
TGAAACAGAATGGATCATCA+AGG + chr1_1:60720379-60720398 Msa0033270:intron 35.0%
TACCAACAACACCCATATTT+GGG - chr1_1:60720417-60720436 None:intergenic 35.0%
!! TTGATACTGATTTCCCCAAA+AGG - chr1_1:60720635-60720654 None:intergenic 35.0%
ATCACACTGAAGAGAACAAA+AGG + chr1_1:60720821-60720840 Msa0033270:CDS 35.0%
! CTGCAATGTCATTCTTTTGA+AGG - chr1_1:60720852-60720871 None:intergenic 35.0%
!! TGGAAGTGTTATGGTTAGTA+AGG + chr1_1:60721166-60721185 Msa0033270:three_prime_UTR 35.0%
ATGGTTAGTAAGGTGAACTT+TGG + chr1_1:60721176-60721195 Msa0033270:three_prime_UTR 35.0%
! CACAAGATGTTTCTAAGTAC+AGG + chr1_1:60721398-60721417 Msa0033270:three_prime_UTR 35.0%
GTATTGTGATATTGCAGTAG+TGG + chr1_1:60721436-60721455 Msa0033270:three_prime_UTR 35.0%
! GAAGCATGTGCTGTTATATT+AGG + chr1_1:60721514-60721533 Msa0033270:three_prime_UTR 35.0%
ACCCCAGAATTTAGTGTTTG+TGG + chr1_1:60719233-60719252 Msa0033270:intron 40.0%
GCCACAAACACTAAATTCTG+GGG - chr1_1:60719237-60719256 None:intergenic 40.0%
GGCCACAAACACTAAATTCT+GGG - chr1_1:60719238-60719257 None:intergenic 40.0%
TGGCCACAAACACTAAATTC+TGG - chr1_1:60719239-60719258 None:intergenic 40.0%
!! GTTGGTTGGTAAGACTTGAT+TGG + chr1_1:60719608-60719627 Msa0033270:intron 40.0%
AACAATGTTTATCGCGTAGC+GGG - chr1_1:60720012-60720031 None:intergenic 40.0%
! TGTCGGTGTTTATCATGTAG+TGG - chr1_1:60720165-60720184 None:intergenic 40.0%
CTAGAATGCTATAGCATACC+AGG - chr1_1:60720235-60720254 None:intergenic 40.0%
TCAATTCCTGCACTTCACAT+GGG - chr1_1:60720343-60720362 None:intergenic 40.0%
ATCAATTCCTGCACTTCACA+TGG - chr1_1:60720344-60720363 None:intergenic 40.0%
AGAATGGATCATCAAGGTCA+TGG + chr1_1:60720385-60720404 Msa0033270:intron 40.0%
TCATGGCCAAAACCCAAATA+TGG + chr1_1:60720402-60720421 Msa0033270:CDS 40.0%
CATGGCCAAAACCCAAATAT+GGG + chr1_1:60720403-60720422 Msa0033270:CDS 40.0%
!! CAACACCCATATTTGGGTTT+TGG - chr1_1:60720411-60720430 None:intergenic 40.0%
! AACCCAAATATGGGTGTTGT+TGG + chr1_1:60720412-60720431 Msa0033270:CDS 40.0%
CTACCAACAACACCCATATT+TGG - chr1_1:60720418-60720437 None:intergenic 40.0%
!! AATATGGGTGTTGTTGGTAG+TGG + chr1_1:60720418-60720437 Msa0033270:CDS 40.0%
!! TGTTGTTGGTAGTGGACAAA+TGG + chr1_1:60720426-60720445 Msa0033270:CDS 40.0%
!! GTATGGATTAGAACCGTATG+GGG - chr1_1:60720452-60720471 None:intergenic 40.0%
!! GGTATGGATTAGAACCGTAT+GGG - chr1_1:60720453-60720472 None:intergenic 40.0%
!! TGGTATGGATTAGAACCGTA+TGG - chr1_1:60720454-60720473 None:intergenic 40.0%
! AACAACAACTCCAGACCTTT+TGG + chr1_1:60720617-60720636 Msa0033270:CDS 40.0%
! ACAACAACTCCAGACCTTTT+GGG + chr1_1:60720618-60720637 Msa0033270:CDS 40.0%
ATCTTCTTGATCCTTGCCAA+GGG - chr1_1:60720697-60720716 None:intergenic 40.0%
AATCTTCTTGATCCTTGCCA+AGG - chr1_1:60720698-60720717 None:intergenic 40.0%
GAAGATTATGAAGGCTGATG+AGG + chr1_1:60720711-60720730 Msa0033270:CDS 40.0%
TGTGAAGATGATATCAGCTG+AGG + chr1_1:60720735-60720754 Msa0033270:CDS 40.0%
GGCGTGTGAAATGTTCATAT+TGG + chr1_1:60720777-60720796 Msa0033270:CDS 40.0%
CAATAAGGAATTCCATCAGC+AGG - chr1_1:60720985-60721004 None:intergenic 40.0%
TTGAGAACACACTGAACATG+AGG + chr1_1:60721549-60721568 Msa0033270:three_prime_UTR 40.0%
CGTTTCGGAATGTTCTCGTA+CGG - chr1_1:60719020-60719039 None:intergenic 45.0%
AAGCATCACCACTCAACACA+AGG + chr1_1:60719103-60719122 Msa0033270:five_prime_UTR 45.0%
! AGTTGGTACCTTGTGTTGAG+TGG - chr1_1:60719114-60719133 None:intergenic 45.0%
AGTACGCGAATTGCAAGAGT+TGG - chr1_1:60719131-60719150 None:intergenic 45.0%
!! TGTTTTTCATGGTGCTGGGA+AGG + chr1_1:60719520-60719539 Msa0033270:intron 45.0%
!! GTTTTTCATGGTGCTGGGAA+GGG + chr1_1:60719521-60719540 Msa0033270:intron 45.0%
!! TTTTTCATGGTGCTGGGAAG+GGG + chr1_1:60719522-60719541 Msa0033270:intron 45.0%
ACAATGTTTATCGCGTAGCG+GGG - chr1_1:60720011-60720030 None:intergenic 45.0%
TCCACAATTGACACCATTGG+CGG - chr1_1:60720115-60720134 None:intergenic 45.0%
CAGTCCACAATTGACACCAT+TGG - chr1_1:60720118-60720137 None:intergenic 45.0%
! ATGGTGTCAATTGTGGACTG+CGG + chr1_1:60720118-60720137 Msa0033270:intron 45.0%
CAATTCCTGCACTTCACATG+GGG - chr1_1:60720342-60720361 None:intergenic 45.0%
GGTTGAATAGTCCCAACAGA+GGG - chr1_1:60720514-60720533 None:intergenic 45.0%
AGGTTGAATAGTCCCAACAG+AGG - chr1_1:60720515-60720534 None:intergenic 45.0%
TCTGTTGGGACTATTCAACC+TGG + chr1_1:60720514-60720533 Msa0033270:CDS 45.0%
! CAACAACTCCAGACCTTTTG+GGG + chr1_1:60720619-60720638 Msa0033270:CDS 45.0%
! ACTGATTTCCCCAAAAGGTC+TGG - chr1_1:60720630-60720649 None:intergenic 45.0%
TGGAGTTAACCTTGCGTTCT+TGG + chr1_1:60720797-60720816 Msa0033270:CDS 45.0%
AGTGTGATTCCAAGAACGCA+AGG - chr1_1:60720809-60720828 None:intergenic 45.0%
TCTTGGTTGACATTGTGCCT+AGG + chr1_1:60720902-60720921 Msa0033270:CDS 45.0%
CTTGGTTGACATTGTGCCTA+GGG + chr1_1:60720903-60720922 Msa0033270:CDS 45.0%
! TCATCTTTCAAGTCCTCCCT+AGG - chr1_1:60720922-60720941 None:intergenic 45.0%
! GAAGTGCTTGCATTACCAAG+AGG + chr1_1:60720940-60720959 Msa0033270:CDS 45.0%
TGAGGCGGCATATAGCAATA+AGG - chr1_1:60721000-60721019 None:intergenic 45.0%
GGATTATGCATCATGAGTCC+AGG - chr1_1:60721045-60721064 None:intergenic 45.0%
GACTGTGGAGCATACATGTT+GGG - chr1_1:60721066-60721085 None:intergenic 45.0%
AGACTGTGGAGCATACATGT+TGG - chr1_1:60721067-60721086 None:intergenic 45.0%
TCCACAGTCTCATCCATACA+TGG + chr1_1:60721077-60721096 Msa0033270:CDS 45.0%
! CACATTTGAGGTGCCATGTA+TGG - chr1_1:60721093-60721112 None:intergenic 45.0%
CATACATGGCACCTCAAATG+TGG + chr1_1:60721091-60721110 Msa0033270:CDS 45.0%
CATTCCGAAACGTGCTTCTG+TGG + chr1_1:60719028-60719047 Msa0033270:five_prime_UTR 50.0%
! AACGCCACAGAAGCACGTTT+CGG - chr1_1:60719035-60719054 None:intergenic 50.0%
TCGAACACTCTCGTGCAATC+AGG + chr1_1:60719062-60719081 Msa0033270:five_prime_UTR 50.0%
ATCCGCAAACGACACCAGTA+GGG - chr1_1:60719964-60719983 None:intergenic 50.0%
AATCCGCAAACGACACCAGT+AGG - chr1_1:60719965-60719984 None:intergenic 50.0%
TCAAATCACAGCTCCGCCAA+TGG + chr1_1:60720099-60720118 Msa0033270:intron 50.0%
! TCCGCCAATGGTGTCAATTG+TGG + chr1_1:60720111-60720130 Msa0033270:intron 50.0%
AGTGGACAAATGGCCCCATA+CGG + chr1_1:60720436-60720455 Msa0033270:CDS 50.0%
GTCATTGGGTTTGGCTGGTA+TGG - chr1_1:60720469-60720488 None:intergenic 50.0%
TACCAGCCAAACCCAATGAC+TGG + chr1_1:60720469-60720488 Msa0033270:CDS 50.0%
ACCAGCCAAACCCAATGACT+GGG + chr1_1:60720470-60720489 Msa0033270:CDS 50.0%
GGATGGTTGTTCCCTCTGTT+GGG + chr1_1:60720500-60720519 Msa0033270:CDS 50.0%
GTTGGGACTATTCAACCTGG+TGG + chr1_1:60720517-60720536 Msa0033270:CDS 50.0%
TCAACCTGATGGAACTCAGC+TGG + chr1_1:60720540-60720559 Msa0033270:CDS 50.0%
CAACCTGATGGAACTCAGCT+GGG + chr1_1:60720541-60720560 Msa0033270:CDS 50.0%
CAAAAACCACAGCCTTCCCT+TGG + chr1_1:60720678-60720697 Msa0033270:CDS 50.0%
TCTTGATCCTTGCCAAGGGA+AGG - chr1_1:60720693-60720712 None:intergenic 50.0%
CACGCCCTTGCAAATACAAC+CGG - chr1_1:60720763-60720782 None:intergenic 50.0%
GACATTGCAGCAGCAATCAC+AGG + chr1_1:60720862-60720881 Msa0033270:CDS 50.0%
!!! GCTTGCTGTGCATTTTGAGG+CGG - chr1_1:60721015-60721034 None:intergenic 50.0%
!!! CCAGCTTGCTGTGCATTTTG+AGG - chr1_1:60721018-60721037 None:intergenic 50.0%
CCTCAAAATGCACAGCAAGC+TGG + chr1_1:60721015-60721034 Msa0033270:CDS 50.0%
GCCATGTATGGATGAGACTG+TGG - chr1_1:60721081-60721100 None:intergenic 50.0%
! TTGTTGTTGCTCCGGTGGTT+GGG - chr1_1:60721118-60721137 None:intergenic 50.0%
! ATTGTTGTTGCTCCGGTGGT+TGG - chr1_1:60721119-60721138 None:intergenic 50.0%
! GAAGGCGATTGTTGTTGCTC+CGG - chr1_1:60721126-60721145 None:intergenic 50.0%
! TTGAGTGGTGATGCTTGCCC+TGG - chr1_1:60719099-60719118 None:intergenic 55.0%
! TTTTGTCCCTCGTAGGTCGC+AGG - chr1_1:60719734-60719753 None:intergenic 55.0%
GACCTACGAGGGACAAAACG+CGG + chr1_1:60719736-60719755 Msa0033270:intron 55.0%
ACCTACGAGGGACAAAACGC+GGG + chr1_1:60719737-60719756 Msa0033270:intron 55.0%
TCCGCAAACGACACCAGTAG+GGG - chr1_1:60719963-60719982 None:intergenic 55.0%
!! CTGGTGTCGTTTGCGGATTG+CGG + chr1_1:60719966-60719985 Msa0033270:intron 55.0%
TTTGGCCCCATGTGAAGTGC+AGG + chr1_1:60720334-60720353 Msa0033270:intron 55.0%
ACCCAGTCATTGGGTTTGGC+TGG - chr1_1:60720474-60720493 None:intergenic 55.0%
GGTGACCCAGTCATTGGGTT+TGG - chr1_1:60720478-60720497 None:intergenic 55.0%
AACCCAATGACTGGGTCACC+TGG + chr1_1:60720478-60720497 Msa0033270:CDS 55.0%
ACCCAATGACTGGGTCACCT+GGG + chr1_1:60720479-60720498 Msa0033270:CDS 55.0%
TCCCAGGTGACCCAGTCATT+GGG - chr1_1:60720483-60720502 None:intergenic 55.0%
ATCCCAGGTGACCCAGTCAT+TGG - chr1_1:60720484-60720503 None:intergenic 55.0%
AATGACTGGGTCACCTGGGA+TGG + chr1_1:60720483-60720502 Msa0033270:CDS 55.0%
ACAGAGGGAACAACCATCCC+AGG - chr1_1:60720499-60720518 None:intergenic 55.0%
GGGATGGTTGTTCCCTCTGT+TGG + chr1_1:60720499-60720518 Msa0033270:CDS 55.0%
CAACCTGGTGGTCAACCTGA+TGG + chr1_1:60720529-60720548 Msa0033270:CDS 55.0%
!! GTTCCATCAGGTTGACCACC+AGG - chr1_1:60720535-60720554 None:intergenic 55.0%
TGTCCCAGCTGAGTTCCATC+AGG - chr1_1:60720547-60720566 None:intergenic 55.0%
GATGATATCAGCTGAGGCGC+CGG + chr1_1:60720741-60720760 Msa0033270:CDS 55.0%
!! AGGCGCCGGTTGTATTTGCA+AGG + chr1_1:60720755-60720774 Msa0033270:CDS 55.0%
!! GGCGCCGGTTGTATTTGCAA+GGG + chr1_1:60720756-60720775 Msa0033270:CDS 55.0%
GGTTGACATTGTGCCTAGGG+AGG + chr1_1:60720906-60720925 Msa0033270:CDS 55.0%
GCACAGCAAGCTGGAAATCC+TGG + chr1_1:60721024-60721043 Msa0033270:CDS 55.0%
TCAAATGTGGCCCCAACCAC+CGG + chr1_1:60721104-60721123 Msa0033270:CDS 55.0%
TGTTGTTGCTCCGGTGGTTG+GGG - chr1_1:60721117-60721136 None:intergenic 55.0%
TGCTGGGAAGGGGTGAAGCT+AGG + chr1_1:60719532-60719551 Msa0033270:intron 60.0%
ACTCGATCCTGCGACCTACG+AGG + chr1_1:60719724-60719743 Msa0033270:intron 60.0%
CTCGATCCTGCGACCTACGA+GGG + chr1_1:60719725-60719744 Msa0033270:intron 60.0%
! ACCCGCGTTTTGTCCCTCGT+AGG - chr1_1:60719741-60719760 None:intergenic 60.0%
CCGCAAACGACACCAGTAGG+GGG - chr1_1:60719962-60719981 None:intergenic 60.0%
!! CCCCCTACTGGTGTCGTTTG+CGG + chr1_1:60719959-60719978 Msa0033270:intron 60.0%
ACCACAGCCTTCCCTTGGCA+AGG + chr1_1:60720683-60720702 Msa0033270:CDS 60.0%
TCCTTGCCAAGGGAAGGCTG+TGG - chr1_1:60720687-60720706 None:intergenic 60.0%
GGTGGTTGGGGCCACATTTG+AGG - chr1_1:60721105-60721124 None:intergenic 60.0%
! GGCGATTGTTGTTGCTCCGG+TGG - chr1_1:60721123-60721142 None:intergenic 60.0%
CCAGTAGGGGGAGCTAGCCA+TGG - chr1_1:60719950-60719969 None:intergenic 65.0%
! CCATGGCTAGCTCCCCCTAC+TGG + chr1_1:60719947-60719966 Msa0033270:intron 65.0%
!! CCAGCAGGCATCGGTCCTCT+TGG - chr1_1:60720958-60720977 None:intergenic 65.0%
CCAAGAGGACCGATGCCTGC+TGG + chr1_1:60720955-60720974 Msa0033270:CDS 65.0%
AGAGGACCGATGCCTGCTGG+AGG + chr1_1:60720958-60720977 Msa0033270:CDS 65.0%
GCAGGTCCTCCAGCAGGCAT+CGG - chr1_1:60720967-60720986 None:intergenic 65.0%
CCATCAGCAGGTCCTCCAGC+AGG - chr1_1:60720973-60720992 None:intergenic 65.0%
CCTGCTGGAGGACCTGCTGA+TGG + chr1_1:60720970-60720989 Msa0033270:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60719013 60721611 60719013 ID=Msa0033270;Name=Msa0033270
chr1_1 mRNA 60719013 60721611 60719013 ID=Msa0033270-mRNA-1;Parent=Msa0033270;Name=Msa0033270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=112|0|0.5|1|0|0.5|2|456|255
chr1_1 exon 60719013 60719124 60719013 ID=Msa0033270-mRNA-1:exon:19016;Parent=Msa0033270-mRNA-1
chr1_1 exon 60720388 60721611 60720388 ID=Msa0033270-mRNA-1:exon:19017;Parent=Msa0033270-mRNA-1
chr1_1 five_prime_UTR 60719013 60719124 60719013 ID=Msa0033270-mRNA-1:five_prime_utr;Parent=Msa0033270-mRNA-1
chr1_1 CDS 60720388 60721155 60720388 ID=Msa0033270-mRNA-1:cds;Parent=Msa0033270-mRNA-1
chr1_1 three_prime_UTR 60721156 60721611 60721156 ID=Msa0033270-mRNA-1:three_prime_utr;Parent=Msa0033270-mRNA-1
Gene Sequence

>Msa0033270

ATGGATCATCAAGGTCATGGCCAAAACCCAAATATGGGTGTTGTTGGTAGTGGACAAATGGCCCCATACGGTTCTAATCCATACCAGCCAAACCCAATGACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGACTATTCAACCTGGTGGTCAACCTGATGGAACTCAGCTGGGACAACATCAGCTTGCTTATCAGCACATTCATCAGCAGCAACAGCAGCAGCAACAACAACAACTCCAGACCTTTTGGGGAAATCAGTATCAAGAAATTGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCTGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGTGAAATGTTCATATTGGAGTTAACCTTGCGTTCTTGGAATCACACTGAAGAGAACAAAAGGCGAACCCTTCAAAAGAATGACATTGCAGCAGCAATCACAGGAACTGATATCTTTGATTTCTTGGTTGACATTGTGCCTAGGGAGGACTTGAAAGATGAAGTGCTTGCATTACCAAGAGGACCGATGCCTGCTGGAGGACCTGCTGATGGAATTCCTTATTGCTATATGCCGCCTCAAAATGCACAGCAAGCTGGAAATCCTGGACTCATGATGCATAATCCCAACATGTATGCTCCACAGTCTCATCCATACATGGCACCTCAAATGTGGCCCCAACCACCGGAGCAACAACAATCGCCTTCAGATCATTAG
Protein sequence

>Msa0033270

MDHQGHGQNPNMGVVGSGQMAPYGSNPYQPNPMTGSPGMVVPSVGTIQPGGQPDGTQLGQHQLAYQHIHQQQQQQQQQQLQTFWGNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVKMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITGTDIFDFLVDIVPREDLKDEVLALPRGPMPAGGPADGIPYCYMPPQNAQQAGNPGLMMHNPNMYAPQSHPYMAPQMWPQPPEQQQSPSDH*