AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0035040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035040 MtrunA17_Chr1g0193861 100.000 157 0 0 1 157 1 157 1.08e-117 329
Msa0035040 MtrunA17_Chr7g0270851 62.893 159 40 5 1 157 1 142 2.96e-54 168
Msa0035040 MtrunA17_Chr4g0069561 50.427 117 57 1 41 157 52 167 2.64e-29 105
Msa0035040 MtrunA17_Chr1g0183251 48.315 89 29 3 85 156 147 235 9.40e-17 75.1
Msa0035040 MtrunA17_Chr1g0184801 36.364 143 71 5 16 157 58 181 7.00e-16 71.6
Msa0035040 MtrunA17_Chr1g0166011 31.613 155 82 4 9 157 26 162 2.53e-15 69.7
Msa0035040 MtrunA17_Chr1g0187961 43.421 76 42 1 82 157 84 158 1.82e-14 67.0
Msa0035040 MtrunA17_Chr7g0260621 38.542 96 54 3 64 156 87 180 1.69e-13 65.1
Msa0035040 MtrunA17_Chr5g0428141 42.045 88 36 2 83 157 230 315 1.10e-12 64.3
Msa0035040 MtrunA17_Chr5g0411501 41.111 90 39 2 82 157 214 303 1.18e-12 64.3
Msa0035040 MtrunA17_Chr6g0486121 33.898 118 54 3 64 157 105 222 3.77e-12 62.0
Msa0035040 MtrunA17_Chr1g0184791 36.559 93 46 1 78 157 121 213 5.75e-12 61.6
Msa0035040 MtrunA17_Chr8g0390281 36.082 97 47 1 76 157 165 261 1.20e-11 61.2
Msa0035040 MtrunA17_Chr7g0260641 35.556 90 46 1 80 157 129 218 1.28e-11 60.8
Msa0035040 MtrunA17_Chr2g0330951 32.292 96 48 2 78 156 142 237 1.43e-11 60.8
Msa0035040 MtrunA17_Chr2g0331411 36.364 88 42 1 83 156 206 293 2.56e-11 60.5
Msa0035040 MtrunA17_Chr8g0340731 30.851 94 50 1 78 156 150 243 4.63e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035040 AT3G62100.2 46.897 145 62 5 20 157 19 155 2.70e-30 107
Msa0035040 AT3G62100.1 46.897 145 62 5 20 157 19 155 3.61e-30 108
Msa0035040 AT2G46990.2 43.939 132 62 4 36 157 28 157 4.83e-27 99.8
Msa0035040 AT2G46990.1 43.939 132 62 4 36 157 28 157 6.42e-27 99.8
Msa0035040 AT3G17600.1 42.400 125 53 5 42 157 27 141 1.40e-24 93.2
Msa0035040 AT5G25890.1 45.946 74 33 1 84 157 81 147 1.25e-17 75.9
Msa0035040 AT4G28640.3 46.237 93 39 2 75 156 128 220 4.33e-17 75.9
Msa0035040 AT4G28640.1 46.237 93 39 2 75 156 128 220 4.83e-17 75.9
Msa0035040 AT4G28640.2 46.237 93 39 2 75 156 128 220 1.81e-16 75.1
Msa0035040 AT1G04550.2 47.436 78 34 2 85 156 126 202 2.17e-14 68.6
Msa0035040 AT3G16500.1 34.286 105 51 2 71 157 132 236 2.26e-14 68.9
Msa0035040 AT5G65670.1 40.449 89 39 1 83 157 216 304 2.75e-14 69.3
Msa0035040 AT3G16500.2 34.286 105 51 2 71 157 132 236 2.89e-14 68.6
Msa0035040 AT5G65670.2 40.449 89 39 1 83 157 216 304 2.89e-14 68.9
Msa0035040 AT1G04240.1 44.000 75 41 1 83 157 92 165 4.01e-14 67.0
Msa0035040 AT1G51950.1 38.372 86 42 2 83 157 149 234 4.42e-14 68.2
Msa0035040 AT2G33310.2 43.750 80 37 1 85 156 131 210 5.37e-14 67.4
Msa0035040 AT2G33310.3 43.750 80 37 1 85 156 130 209 6.12e-14 67.4
Msa0035040 AT2G33310.1 43.750 80 37 1 85 156 130 209 6.12e-14 67.4
Msa0035040 AT1G04240.2 44.595 74 40 1 84 157 193 265 8.52e-14 67.4
Msa0035040 AT3G23030.1 38.298 94 51 2 64 157 64 150 8.85e-14 65.9
Msa0035040 AT3G23030.2 38.298 94 51 2 64 157 151 237 2.45e-13 65.9
Msa0035040 AT4G14560.1 43.836 73 40 1 85 157 76 147 4.11e-13 63.9
Msa0035040 AT2G22670.5 40.449 89 39 2 83 157 199 287 1.91e-12 63.9
Msa0035040 AT2G22670.2 40.449 89 39 2 83 157 199 287 1.91e-12 63.9
Msa0035040 AT2G22670.3 40.449 89 39 2 83 157 199 287 1.95e-12 63.9
Msa0035040 AT2G22670.1 40.449 89 39 2 83 157 199 287 1.95e-12 63.9
Msa0035040 AT2G22670.4 40.449 89 39 2 83 157 216 304 2.14e-12 63.9
Msa0035040 AT5G43700.1 43.836 73 40 1 85 157 90 161 2.41e-12 62.0
Msa0035040 AT4G29080.1 38.202 89 41 2 83 157 185 273 2.71e-12 63.5
Msa0035040 AT1G52830.1 34.043 94 59 2 64 157 74 164 5.21e-12 61.2
Msa0035040 AT1G15050.2 33.898 118 60 4 42 156 29 131 5.14e-11 57.8
Msa0035040 AT1G15050.1 33.898 118 60 4 42 156 61 163 5.56e-11 58.5

Find 45 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTGGAATTGTAGAATT+TGG 0.173695 1_1:+63052793 None:intergenic
TCTTTATTATTTAAACCTTA+AGG 0.333508 1_1:-63052857 Msa0035040:five_prime_UTR
TCACCGCCTTGTTGCTGAAT+TGG 0.337037 1_1:+63052496 None:intergenic
AAGAATAAGCCGCCACCTTA+AGG 0.358276 1_1:+63052842 None:intergenic
TGGAGAAGGAGACTTGATTA+TGG 0.360172 1_1:-63052166 Msa0035040:CDS
CTCAATTTGCTAGCTCATAA+TGG 0.373904 1_1:-63052397 Msa0035040:CDS
AAGGAGACTTGATTATGGTT+GGG 0.404725 1_1:-63052161 Msa0035040:CDS
TACATGGAAGGCATTCCAAT+TGG 0.410476 1_1:-63052427 Msa0035040:CDS
TATGGTTGGGGATGTCCCTT+GGG 0.419336 1_1:-63052148 Msa0035040:CDS
AAATTGAGCTTTCTCCCAAT+TGG 0.430798 1_1:+63052412 None:intergenic
TTATGGTTGGGGATGTCCCT+TGG 0.433671 1_1:-63052149 Msa0035040:CDS
AGGAGACTTGATTATGGTTG+GGG 0.442249 1_1:-63052160 Msa0035040:CDS
TACAATTCCACAATGGGAAA+AGG 0.446881 1_1:-63052785 Msa0035040:exon
GAAGGAGACTTGATTATGGT+TGG 0.447956 1_1:-63052162 Msa0035040:CDS
CCTTCTTCTACTGTTGGAAG+AGG 0.459161 1_1:+63052745 None:intergenic
ACATGGAAGGCATTCCAATT+GGG 0.472520 1_1:-63052426 Msa0035040:CDS
GGAAGAGGAGGAAGAAGAAG+TGG 0.473631 1_1:+63052760 None:intergenic
AAATTCTACAATTCCACAAT+GGG 0.475764 1_1:-63052791 Msa0035040:exon
GATAGAGGATGAAACATTAG+AGG 0.481392 1_1:+63052712 None:intergenic
CTTCTTTGTGAAGGTTTACA+TGG 0.492048 1_1:-63052443 Msa0035040:CDS
AAAGAAAGAAAGAATTGAAG+AGG 0.500027 1_1:+63052883 None:intergenic
CTCAATCCAAGGTTAAGATC+AGG 0.506435 1_1:+63052656 None:intergenic
CATGTAAACCTTCACAAAGA+AGG 0.519682 1_1:+63052444 None:intergenic
TTTGTGAAGGTTTACATGGA+AGG 0.523480 1_1:-63052439 Msa0035040:CDS
ATGTTTCCTTCTTCTACTGT+TGG 0.532208 1_1:+63052739 None:intergenic
TTTGTCATATTAGGGGACAT+TGG 0.532866 1_1:-63052522 Msa0035040:intron
TTATTTAAACCTTAAGGTGG+CGG 0.534814 1_1:-63052851 Msa0035040:five_prime_UTR
CCTCTTCCAACAGTAGAAGA+AGG 0.535777 1_1:-63052745 Msa0035040:CDS
GTAATGGCCCTTACATAGAA+TGG 0.537441 1_1:+63052330 None:intergenic
GTTAAGTAAACTTACTCCCA+AGG 0.550745 1_1:+63052132 None:intergenic
TTCTTTAACATACCTTGAGG+TGG 0.555886 1_1:+63052601 None:intergenic
TCTTCTACTGTTGGAAGAGG+AGG 0.561746 1_1:+63052748 None:intergenic
CAACAGTGCCTTCTTTGTGA+AGG 0.564852 1_1:-63052452 Msa0035040:CDS
TCATAATGGCTATCATGAGT+TGG 0.572244 1_1:-63052383 Msa0035040:CDS
CATGTGTTAACTTATGAAGA+TGG 0.578380 1_1:-63052186 Msa0035040:CDS
ATCAACATATTGTTGAGTGG+TGG 0.579143 1_1:+63052628 None:intergenic
CAAATTCTACAATTCCACAA+TGG 0.580800 1_1:-63052792 Msa0035040:exon
TTATTATTTAAACCTTAAGG+TGG 0.581557 1_1:-63052854 Msa0035040:five_prime_UTR
ATGTTGATTATTCCACCTCA+AGG 0.585626 1_1:-63052613 Msa0035040:intron
TTAACTTATGAAGATGGAGA+AGG 0.590910 1_1:-63052180 Msa0035040:CDS
TTAAGTAAACTTACTCCCAA+GGG 0.604202 1_1:+63052133 None:intergenic
TGGTGGAAATGCTCAATCCA+AGG 0.621212 1_1:+63052645 None:intergenic
TGGCAACCAATTCAGCAACA+AGG 0.686483 1_1:-63052502 Msa0035040:CDS
ATAATCAACATATTGTTGAG+TGG 0.703279 1_1:+63052625 None:intergenic
CAACCAATTCAGCAACAAGG+CGG 0.746050 1_1:-63052499 Msa0035040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTTATTATTTAAACCTTA+AGG - chr1_1:63052192-63052211 Msa0035040:CDS 15.0%
!!! ATTTTTACTTCTTTTTTTGT+TGG - chr1_1:63052755-63052774 Msa0035040:CDS 15.0%
!! TCTTTATTATTTAAACCTTA+AGG - chr1_1:63052192-63052211 Msa0035040:CDS 15.0%
!!! ATTTTTACTTCTTTTTTTGT+TGG - chr1_1:63052755-63052774 Msa0035040:CDS 15.0%
!! TTATTATTTAAACCTTAAGG+TGG - chr1_1:63052195-63052214 Msa0035040:CDS 20.0%
!!! ATTGTGTTATTTGTCATATT+AGG - chr1_1:63052518-63052537 Msa0035040:intron 20.0%
!!! TTGTGTTATTTGTCATATTA+GGG - chr1_1:63052519-63052538 Msa0035040:intron 20.0%
!! AAGTAAAAATGCATATATGA+GGG + chr1_1:63052745-63052764 None:intergenic 20.0%
!! TTATTATTTAAACCTTAAGG+TGG - chr1_1:63052195-63052214 Msa0035040:CDS 20.0%
!!! ATTGTGTTATTTGTCATATT+AGG - chr1_1:63052518-63052537 Msa0035040:intron 20.0%
!!! TTGTGTTATTTGTCATATTA+GGG - chr1_1:63052519-63052538 Msa0035040:intron 20.0%
!! AAGTAAAAATGCATATATGA+GGG + chr1_1:63052745-63052764 None:intergenic 20.0%
! AAAGAAAGAAAGAATTGAAG+AGG + chr1_1:63052169-63052188 None:intergenic 25.0%
! AAATTCTACAATTCCACAAT+GGG - chr1_1:63052258-63052277 Msa0035040:intron 25.0%
! ATAATCAACATATTGTTGAG+TGG + chr1_1:63052427-63052446 None:intergenic 25.0%
!! TTTTTCTTTAACATACCTTG+AGG + chr1_1:63052454-63052473 None:intergenic 25.0%
!!! TGTGTTATTTGTCATATTAG+GGG - chr1_1:63052520-63052539 Msa0035040:intron 25.0%
! AGTAAAAATGCATATATGAG+GGG + chr1_1:63052744-63052763 None:intergenic 25.0%
! GAAGTAAAAATGCATATATG+AGG + chr1_1:63052746-63052765 None:intergenic 25.0%
!! TGTTGGTAAAATGAGTAATA+TGG - chr1_1:63052772-63052791 Msa0035040:CDS 25.0%
! AAAGAAAGAAAGAATTGAAG+AGG + chr1_1:63052169-63052188 None:intergenic 25.0%
! AAATTCTACAATTCCACAAT+GGG - chr1_1:63052258-63052277 Msa0035040:intron 25.0%
! ATAATCAACATATTGTTGAG+TGG + chr1_1:63052427-63052446 None:intergenic 25.0%
!! TTTTTCTTTAACATACCTTG+AGG + chr1_1:63052454-63052473 None:intergenic 25.0%
!!! TGTGTTATTTGTCATATTAG+GGG - chr1_1:63052520-63052539 Msa0035040:intron 25.0%
! AGTAAAAATGCATATATGAG+GGG + chr1_1:63052744-63052763 None:intergenic 25.0%
! GAAGTAAAAATGCATATATG+AGG + chr1_1:63052746-63052765 None:intergenic 25.0%
!! TGTTGGTAAAATGAGTAATA+TGG - chr1_1:63052772-63052791 Msa0035040:CDS 25.0%
TTATTTAAACCTTAAGGTGG+CGG - chr1_1:63052198-63052217 Msa0035040:CDS 30.0%
CATTGTGGAATTGTAGAATT+TGG + chr1_1:63052259-63052278 None:intergenic 30.0%
CAAATTCTACAATTCCACAA+TGG - chr1_1:63052257-63052276 Msa0035040:intron 30.0%
GACACAAACATAGTAGTTTA+AGG + chr1_1:63052483-63052502 None:intergenic 30.0%
!!! TGTGACTTTGTTATTTTACC+AGG - chr1_1:63052803-63052822 Msa0035040:five_prime_UTR 30.0%
!!! GTGACTTTGTTATTTTACCA+GGG - chr1_1:63052804-63052823 Msa0035040:five_prime_UTR 30.0%
!!! TGACTTTGTTATTTTACCAG+GGG - chr1_1:63052805-63052824 Msa0035040:five_prime_UTR 30.0%
CATGTGTTAACTTATGAAGA+TGG - chr1_1:63052863-63052882 Msa0035040:five_prime_UTR 30.0%
TTAACTTATGAAGATGGAGA+AGG - chr1_1:63052869-63052888 Msa0035040:five_prime_UTR 30.0%
TTATTTAAACCTTAAGGTGG+CGG - chr1_1:63052198-63052217 Msa0035040:CDS 30.0%
CATTGTGGAATTGTAGAATT+TGG + chr1_1:63052259-63052278 None:intergenic 30.0%
CAAATTCTACAATTCCACAA+TGG - chr1_1:63052257-63052276 Msa0035040:intron 30.0%
GACACAAACATAGTAGTTTA+AGG + chr1_1:63052483-63052502 None:intergenic 30.0%
!!! TGTGACTTTGTTATTTTACC+AGG - chr1_1:63052803-63052822 Msa0035040:five_prime_UTR 30.0%
!!! GTGACTTTGTTATTTTACCA+GGG - chr1_1:63052804-63052823 Msa0035040:five_prime_UTR 30.0%
!!! TGACTTTGTTATTTTACCAG+GGG - chr1_1:63052805-63052824 Msa0035040:five_prime_UTR 30.0%
CATGTGTTAACTTATGAAGA+TGG - chr1_1:63052863-63052882 Msa0035040:five_prime_UTR 30.0%
TTAACTTATGAAGATGGAGA+AGG - chr1_1:63052869-63052888 Msa0035040:five_prime_UTR 30.0%
TACAATTCCACAATGGGAAA+AGG - chr1_1:63052264-63052283 Msa0035040:intron 35.0%
ATGTTTCCTTCTTCTACTGT+TGG + chr1_1:63052313-63052332 None:intergenic 35.0%
GATAGAGGATGAAACATTAG+AGG + chr1_1:63052340-63052359 None:intergenic 35.0%
!! AGGATTTTGATGTGTGATAG+AGG + chr1_1:63052355-63052374 None:intergenic 35.0%
GGAAAAAGATGTTGTTGATC+AGG + chr1_1:63052375-63052394 None:intergenic 35.0%
! CTTTTTCCTGATCTTAACCT+TGG - chr1_1:63052387-63052406 Msa0035040:CDS 35.0%
ATCAACATATTGTTGAGTGG+TGG + chr1_1:63052424-63052443 None:intergenic 35.0%
! ATGTTGATTATTCCACCTCA+AGG - chr1_1:63052436-63052455 Msa0035040:CDS 35.0%
TTCTTTAACATACCTTGAGG+TGG + chr1_1:63052451-63052470 None:intergenic 35.0%
TTTGTCATATTAGGGGACAT+TGG - chr1_1:63052527-63052546 Msa0035040:intron 35.0%
CATGTAAACCTTCACAAAGA+AGG + chr1_1:63052608-63052627 None:intergenic 35.0%
CTTCTTTGTGAAGGTTTACA+TGG - chr1_1:63052606-63052625 Msa0035040:intron 35.0%
TTTGTGAAGGTTTACATGGA+AGG - chr1_1:63052610-63052629 Msa0035040:intron 35.0%
AAATTGAGCTTTCTCCCAAT+TGG + chr1_1:63052640-63052659 None:intergenic 35.0%
!! CTCAATTTGCTAGCTCATAA+TGG - chr1_1:63052652-63052671 Msa0035040:CDS 35.0%
TCATAATGGCTATCATGAGT+TGG - chr1_1:63052666-63052685 Msa0035040:CDS 35.0%
TGACACTACCATTCTATGTA+AGG - chr1_1:63052711-63052730 Msa0035040:CDS 35.0%
GACACTACCATTCTATGTAA+GGG - chr1_1:63052712-63052731 Msa0035040:CDS 35.0%
AATGCATATATGAGGGGTAA+TGG + chr1_1:63052738-63052757 None:intergenic 35.0%
!!! GACTTTGTTATTTTACCAGG+GGG - chr1_1:63052806-63052825 Msa0035040:five_prime_UTR 35.0%
!!! ACTTTGTTATTTTACCAGGG+GGG - chr1_1:63052807-63052826 Msa0035040:five_prime_UTR 35.0%
AAGGAGACTTGATTATGGTT+GGG - chr1_1:63052888-63052907 Msa0035040:five_prime_UTR 35.0%
TACAATTCCACAATGGGAAA+AGG - chr1_1:63052264-63052283 Msa0035040:intron 35.0%
ATGTTTCCTTCTTCTACTGT+TGG + chr1_1:63052313-63052332 None:intergenic 35.0%
GATAGAGGATGAAACATTAG+AGG + chr1_1:63052340-63052359 None:intergenic 35.0%
!! AGGATTTTGATGTGTGATAG+AGG + chr1_1:63052355-63052374 None:intergenic 35.0%
GGAAAAAGATGTTGTTGATC+AGG + chr1_1:63052375-63052394 None:intergenic 35.0%
! CTTTTTCCTGATCTTAACCT+TGG - chr1_1:63052387-63052406 Msa0035040:CDS 35.0%
ATCAACATATTGTTGAGTGG+TGG + chr1_1:63052424-63052443 None:intergenic 35.0%
! ATGTTGATTATTCCACCTCA+AGG - chr1_1:63052436-63052455 Msa0035040:CDS 35.0%
TTCTTTAACATACCTTGAGG+TGG + chr1_1:63052451-63052470 None:intergenic 35.0%
TTTGTCATATTAGGGGACAT+TGG - chr1_1:63052527-63052546 Msa0035040:intron 35.0%
CATGTAAACCTTCACAAAGA+AGG + chr1_1:63052608-63052627 None:intergenic 35.0%
CTTCTTTGTGAAGGTTTACA+TGG - chr1_1:63052606-63052625 Msa0035040:intron 35.0%
TTTGTGAAGGTTTACATGGA+AGG - chr1_1:63052610-63052629 Msa0035040:intron 35.0%
AAATTGAGCTTTCTCCCAAT+TGG + chr1_1:63052640-63052659 None:intergenic 35.0%
!! CTCAATTTGCTAGCTCATAA+TGG - chr1_1:63052652-63052671 Msa0035040:CDS 35.0%
TCATAATGGCTATCATGAGT+TGG - chr1_1:63052666-63052685 Msa0035040:CDS 35.0%
TGACACTACCATTCTATGTA+AGG - chr1_1:63052711-63052730 Msa0035040:CDS 35.0%
GACACTACCATTCTATGTAA+GGG - chr1_1:63052712-63052731 Msa0035040:CDS 35.0%
AATGCATATATGAGGGGTAA+TGG + chr1_1:63052738-63052757 None:intergenic 35.0%
!!! GACTTTGTTATTTTACCAGG+GGG - chr1_1:63052806-63052825 Msa0035040:five_prime_UTR 35.0%
!!! ACTTTGTTATTTTACCAGGG+GGG - chr1_1:63052807-63052826 Msa0035040:five_prime_UTR 35.0%
AAGGAGACTTGATTATGGTT+GGG - chr1_1:63052888-63052907 Msa0035040:five_prime_UTR 35.0%
CTCAATCCAAGGTTAAGATC+AGG + chr1_1:63052396-63052415 None:intergenic 40.0%
!! TACATGGAAGGCATTCCAAT+TGG - chr1_1:63052622-63052641 Msa0035040:CDS 40.0%
!! ACATGGAAGGCATTCCAATT+GGG - chr1_1:63052623-63052642 Msa0035040:CDS 40.0%
GTAATGGCCCTTACATAGAA+TGG + chr1_1:63052722-63052741 None:intergenic 40.0%
TGGAGAAGGAGACTTGATTA+TGG - chr1_1:63052883-63052902 Msa0035040:five_prime_UTR 40.0%
GAAGGAGACTTGATTATGGT+TGG - chr1_1:63052887-63052906 Msa0035040:five_prime_UTR 40.0%
AGGAGACTTGATTATGGTTG+GGG - chr1_1:63052889-63052908 Msa0035040:five_prime_UTR 40.0%
CTCAATCCAAGGTTAAGATC+AGG + chr1_1:63052396-63052415 None:intergenic 40.0%
!! TACATGGAAGGCATTCCAAT+TGG - chr1_1:63052622-63052641 Msa0035040:CDS 40.0%
!! ACATGGAAGGCATTCCAATT+GGG - chr1_1:63052623-63052642 Msa0035040:CDS 40.0%
GTAATGGCCCTTACATAGAA+TGG + chr1_1:63052722-63052741 None:intergenic 40.0%
TGGAGAAGGAGACTTGATTA+TGG - chr1_1:63052883-63052902 Msa0035040:five_prime_UTR 40.0%
GAAGGAGACTTGATTATGGT+TGG - chr1_1:63052887-63052906 Msa0035040:five_prime_UTR 40.0%
AGGAGACTTGATTATGGTTG+GGG - chr1_1:63052889-63052908 Msa0035040:five_prime_UTR 40.0%
AAGAATAAGCCGCCACCTTA+AGG + chr1_1:63052210-63052229 None:intergenic 45.0%
! AGTGGAACCTTTTCCCATTG+TGG + chr1_1:63052274-63052293 None:intergenic 45.0%
TCTTCTACTGTTGGAAGAGG+AGG + chr1_1:63052304-63052323 None:intergenic 45.0%
CCTTCTTCTACTGTTGGAAG+AGG + chr1_1:63052307-63052326 None:intergenic 45.0%
CCTCTTCCAACAGTAGAAGA+AGG - chr1_1:63052304-63052323 Msa0035040:intron 45.0%
TGGTGGAAATGCTCAATCCA+AGG + chr1_1:63052407-63052426 None:intergenic 45.0%
TGGCAACCAATTCAGCAACA+AGG - chr1_1:63052547-63052566 Msa0035040:intron 45.0%
CAACCAATTCAGCAACAAGG+CGG - chr1_1:63052550-63052569 Msa0035040:intron 45.0%
CAACAGTGCCTTCTTTGTGA+AGG - chr1_1:63052597-63052616 Msa0035040:intron 45.0%
AAGAATAAGCCGCCACCTTA+AGG + chr1_1:63052210-63052229 None:intergenic 45.0%
! AGTGGAACCTTTTCCCATTG+TGG + chr1_1:63052274-63052293 None:intergenic 45.0%
TCTTCTACTGTTGGAAGAGG+AGG + chr1_1:63052304-63052323 None:intergenic 45.0%
CCTTCTTCTACTGTTGGAAG+AGG + chr1_1:63052307-63052326 None:intergenic 45.0%
CCTCTTCCAACAGTAGAAGA+AGG - chr1_1:63052304-63052323 Msa0035040:intron 45.0%
TGGTGGAAATGCTCAATCCA+AGG + chr1_1:63052407-63052426 None:intergenic 45.0%
TGGCAACCAATTCAGCAACA+AGG - chr1_1:63052547-63052566 Msa0035040:intron 45.0%
CAACCAATTCAGCAACAAGG+CGG - chr1_1:63052550-63052569 Msa0035040:intron 45.0%
CAACAGTGCCTTCTTTGTGA+AGG - chr1_1:63052597-63052616 Msa0035040:intron 45.0%
GGAAGAGGAGGAAGAAGAAG+TGG + chr1_1:63052292-63052311 None:intergenic 50.0%
! TCACCGCCTTGTTGCTGAAT+TGG + chr1_1:63052556-63052575 None:intergenic 50.0%
TTTACCAGGGGGGACTGAAA+TGG - chr1_1:63052817-63052836 Msa0035040:five_prime_UTR 50.0%
TTATGGTTGGGGATGTCCCT+TGG - chr1_1:63052900-63052919 Msa0035040:five_prime_UTR 50.0%
TATGGTTGGGGATGTCCCTT+GGG - chr1_1:63052901-63052920 Msa0035040:five_prime_UTR 50.0%
GGAAGAGGAGGAAGAAGAAG+TGG + chr1_1:63052292-63052311 None:intergenic 50.0%
! TCACCGCCTTGTTGCTGAAT+TGG + chr1_1:63052556-63052575 None:intergenic 50.0%
TTTACCAGGGGGGACTGAAA+TGG - chr1_1:63052817-63052836 Msa0035040:five_prime_UTR 50.0%
TTATGGTTGGGGATGTCCCT+TGG - chr1_1:63052900-63052919 Msa0035040:five_prime_UTR 50.0%
TATGGTTGGGGATGTCCCTT+GGG - chr1_1:63052901-63052920 Msa0035040:five_prime_UTR 50.0%
CCATCCATTTCAGTCCCCCC+TGG + chr1_1:63052824-63052843 None:intergenic 60.0%
CCAGGGGGGACTGAAATGGA+TGG - chr1_1:63052821-63052840 Msa0035040:five_prime_UTR 60.0%
CCATCCATTTCAGTCCCCCC+TGG + chr1_1:63052824-63052843 None:intergenic 60.0%
CCAGGGGGGACTGAAATGGA+TGG - chr1_1:63052821-63052840 Msa0035040:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 63052143 63052928 63052143 ID=Msa0035040;Name=Msa0035040
chr1_1 mRNA 63052143 63052928 63052143 ID=Msa0035040-mRNA-1;Parent=Msa0035040;Name=Msa0035040-mRNA-1;_AED=0.07;_eAED=0.07;_QI=133|0.5|0.66|1|1|1|3|0|157
chr1_1 exon 63052143 63052246 63052143 ID=Msa0035040-mRNA-1:exon:20024;Parent=Msa0035040-mRNA-1
chr1_1 exon 63052344 63052531 63052344 ID=Msa0035040-mRNA-1:exon:20023;Parent=Msa0035040-mRNA-1
chr1_1 exon 63052614 63052928 63052614 ID=Msa0035040-mRNA-1:exon:20022;Parent=Msa0035040-mRNA-1
chr1_1 five_prime_UTR 63052796 63052928 63052796 ID=Msa0035040-mRNA-1:five_prime_utr;Parent=Msa0035040-mRNA-1
chr1_1 CDS 63052614 63052795 63052614 ID=Msa0035040-mRNA-1:cds;Parent=Msa0035040-mRNA-1
chr1_1 CDS 63052344 63052531 63052344 ID=Msa0035040-mRNA-1:cds;Parent=Msa0035040-mRNA-1
chr1_1 CDS 63052143 63052246 63052143 ID=Msa0035040-mRNA-1:cds;Parent=Msa0035040-mRNA-1
Gene Sequence

>Msa0035040

ATGGGAAAAGGTTCCACTTCTTCTTCCTCCTCTTCCAACAGTAGAAGAAGGAAACATAATTCCTCTAATGTTTCATCCTCTATCACACATCAAAATCCTGATCAACAACATCTTTTTCCTGATCTTAACCTTGGATTGAGCATTTCCACCACTCAACAATATGTTGATTATTCCACCTCAAGGGGACATTGGCAACCAATTCAGCAACAAGGCGGTGAAGTAAATGATTGCAGTGATTACAACAGTGCCTTCTTTGTGAAGGTTTACATGGAAGGCATTCCAATTGGGAGAAAGCTCAATTTGCTAGCTCATAATGGCTATCATGAGTTGGTTAATACACTTGAACAAATGTTTGACACTACCATTCTATGGGGGACTGAAATGGATGGAGTGCAATCAGAGAGATGTCATGTGTTAACTTATGAAGATGGAGAAGGAGACTTGATTATGGTTGGGGATGTCCCTTGGGAGTAA
Protein sequence

>Msa0035040

MGKGSTSSSSSSNSRRRKHNSSNVSSSITHQNPDQQHLFPDLNLGLSISTTQQYVDYSTSRGHWQPIQQQGGEVNDCSDYNSAFFVKVYMEGIPIGRKLNLLAHNGYHELVNTLEQMFDTTILWGTEMDGVQSERCHVLTYEDGEGDLIMVGDVPWE*