AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0081420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081420 MtrunA17_Chr1g0193861 100.000 176 0 0 1 176 1 176 8.31e-133 368
Msa0081420 MtrunA17_Chr7g0270851 65.537 177 42 5 1 175 1 160 4.60e-67 201
Msa0081420 MtrunA17_Chr4g0069561 54.135 133 60 1 41 173 52 183 5.38e-39 131
Msa0081420 MtrunA17_Chr1g0183251 50.962 104 34 3 85 171 147 250 7.81e-24 94.4
Msa0081420 MtrunA17_Chr1g0184801 38.462 156 76 5 16 170 58 194 1.40e-22 89.7
Msa0081420 MtrunA17_Chr1g0166011 33.333 168 88 4 9 170 26 175 1.69e-21 86.3
Msa0081420 MtrunA17_Chr1g0187961 44.944 89 48 1 82 170 84 171 1.78e-20 83.6
Msa0081420 MtrunA17_Chr7g0260621 41.818 110 59 3 64 170 87 194 1.89e-20 84.0
Msa0081420 MtrunA17_Chr5g0411501 41.818 110 49 3 82 176 214 323 2.59e-18 80.5
Msa0081420 MtrunA17_Chr5g0428141 41.667 108 47 3 83 176 230 335 4.38e-18 80.1
Msa0081420 MtrunA17_Chr2g0330951 35.833 120 51 4 78 174 142 258 7.39e-18 78.6
Msa0081420 MtrunA17_Chr6g0486121 35.878 131 60 3 64 170 105 235 7.85e-18 78.2
Msa0081420 MtrunA17_Chr1g0184791 38.679 106 52 1 78 170 121 226 8.63e-18 77.8
Msa0081420 MtrunA17_Chr8g0366881 41.441 111 48 4 82 176 227 336 1.45e-17 78.6
Msa0081420 MtrunA17_Chr1g0187921 41.509 106 48 3 78 170 113 217 1.83e-17 77.0
Msa0081420 MtrunA17_Chr8g0340731 33.333 108 57 1 78 170 150 257 2.94e-17 77.0
Msa0081420 MtrunA17_Chr7g0260641 37.864 103 52 1 80 170 129 231 2.99e-17 76.6
Msa0081420 MtrunA17_Chr8g0390281 37.607 117 57 2 76 176 165 281 7.48e-17 76.3
Msa0081420 MtrunA17_Chr4g0004181 47.423 97 46 3 82 176 73 166 9.78e-17 73.9
Msa0081420 MtrunA17_Chr2g0331411 37.615 109 53 2 83 176 206 314 2.29e-16 75.1
Msa0081420 MtrunA17_Chr1g0190711 39.796 98 47 3 85 170 137 234 1.45e-15 72.0
Msa0081420 MtrunA17_Chr1g0209181 41.573 89 50 1 85 173 85 171 1.19e-14 68.2
Msa0081420 MtrunA17_Chr2g0332141 34.783 92 52 1 85 168 8 99 2.32e-14 66.2
Msa0081420 MtrunA17_Chr4g0064131 32.773 119 59 4 64 170 96 205 4.77e-13 64.7
Msa0081420 MtrunA17_Chr7g0239061 35.227 88 50 1 81 168 568 648 1.76e-12 64.7
Msa0081420 MtrunA17_Chr2g0276781 32.039 103 56 2 66 168 538 626 4.35e-12 63.5
Msa0081420 MtrunA17_Chr8g0346711 36.585 82 45 1 87 168 562 636 1.86e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081420 AT3G62100.1 49.686 159 65 5 20 171 19 169 7.37e-39 130
Msa0081420 AT2G46990.1 46.259 147 67 4 36 172 28 172 3.66e-35 121
Msa0081420 AT3G17600.1 44.366 142 60 5 42 174 27 158 1.14e-32 114
Msa0081420 AT3G62100.2 46.897 145 62 5 20 157 19 155 5.16e-30 107
Msa0081420 AT2G46990.2 43.939 132 62 4 36 157 28 157 7.37e-27 99.8
Msa0081420 AT5G25890.1 45.745 94 43 2 84 176 81 167 1.43e-23 92.0
Msa0081420 AT4G28640.3 46.729 107 46 2 75 170 128 234 9.53e-23 91.7
Msa0081420 AT4G28640.1 46.729 107 46 2 75 170 128 234 9.94e-23 91.3
Msa0081420 AT4G28640.2 46.729 107 46 2 75 170 128 234 4.69e-22 90.5
Msa0081420 AT3G16500.1 37.288 118 56 2 71 170 132 249 6.13e-21 87.0
Msa0081420 AT3G16500.2 37.288 118 56 2 71 170 132 249 7.43e-21 87.0
Msa0081420 AT1G51950.1 42.157 102 44 3 83 171 149 248 1.22e-20 86.3
Msa0081420 AT1G04550.2 50.000 90 38 2 85 168 126 214 1.95e-20 85.1
Msa0081420 AT2G33310.2 44.681 94 44 1 85 170 131 224 4.61e-20 84.3
Msa0081420 AT2G33310.3 44.681 94 44 1 85 170 130 223 4.68e-20 84.3
Msa0081420 AT2G33310.1 44.681 94 44 1 85 170 130 223 4.68e-20 84.3
Msa0081420 AT5G65670.1 41.284 109 49 2 83 176 216 324 6.71e-20 85.5
Msa0081420 AT5G65670.2 41.284 109 49 2 83 176 216 324 7.82e-20 85.1
Msa0081420 AT3G23030.1 41.121 107 56 2 64 170 64 163 1.30e-19 81.6
Msa0081420 AT1G04240.1 44.318 88 48 1 83 170 92 178 1.31e-19 82.0
Msa0081420 AT1G04240.2 44.318 88 48 1 83 170 192 278 4.55e-19 82.4
Msa0081420 AT1G52830.1 38.596 114 66 3 64 176 74 184 4.56e-19 80.5
Msa0081420 AT3G23030.2 41.121 107 56 2 64 170 151 250 5.23e-19 81.6
Msa0081420 AT5G43700.1 46.512 86 45 1 85 170 90 174 1.26e-18 79.3
Msa0081420 AT4G14560.1 44.186 86 47 1 85 170 76 160 1.11e-17 76.3
Msa0081420 AT3G15540.1 44.086 93 49 2 85 176 98 188 1.46e-17 76.6
Msa0081420 AT4G29080.1 39.216 102 48 2 83 170 185 286 2.00e-17 78.2
Msa0081420 AT1G15050.2 36.364 132 66 4 42 170 29 145 5.69e-17 73.9
Msa0081420 AT1G15050.1 36.364 132 66 4 42 170 61 177 7.68e-17 74.7
Msa0081420 AT2G22670.5 38.532 109 52 3 83 176 199 307 8.40e-16 73.9
Msa0081420 AT2G22670.2 38.532 109 52 3 83 176 199 307 8.40e-16 73.9
Msa0081420 AT2G22670.3 38.889 108 51 3 84 176 200 307 8.52e-16 73.9
Msa0081420 AT2G22670.1 38.889 108 51 3 84 176 200 307 8.52e-16 73.9
Msa0081420 AT2G22670.4 38.532 109 52 3 83 176 216 324 9.67e-16 73.9
Msa0081420 AT4G14550.1 33.588 131 62 5 64 170 80 209 1.20e-15 72.4
Msa0081420 AT4G14550.2 33.588 131 62 5 64 170 80 209 1.66e-15 72.0
Msa0081420 AT1G04250.1 40.000 110 50 4 82 176 109 217 2.13e-15 71.6
Msa0081420 AT1G04100.1 37.864 103 50 2 82 170 150 252 4.15e-15 71.2
Msa0081420 AT3G04730.1 38.776 98 48 3 85 170 120 217 1.28e-14 69.7
Msa0081420 AT3G23050.1 36.735 98 50 3 85 170 126 223 2.64e-14 68.9
Msa0081420 AT1G15580.1 39.535 86 48 2 85 170 76 157 5.06e-14 66.6
Msa0081420 AT4G23980.2 32.692 104 63 1 65 168 503 599 4.58e-13 66.6
Msa0081420 AT4G23980.1 32.692 104 63 1 65 168 505 601 4.85e-13 66.6
Msa0081420 AT2G01200.2 35.644 101 63 1 70 170 85 183 1.18e-11 60.8
Msa0081420 AT2G01200.1 35.644 101 63 1 70 170 85 183 1.18e-11 60.8
Msa0081420 AT4G32280.2 36.782 87 47 2 85 170 119 198 4.60e-11 59.7
Msa0081420 AT4G32280.1 36.782 87 47 2 85 170 161 240 8.15e-11 59.3

Find 60 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTGGAATTGTAGAATT+TGG 0.173695 1_2:+59952728 None:intergenic
TTCACTTCTCTAATTTAAAA+TGG 0.180789 1_2:+59951525 None:intergenic
TAAAATGGACCTAATCTTAA+AGG 0.200614 1_2:+59951540 None:intergenic
TCTTTATTATTTAAACCTTA+AGG 0.333508 1_2:-59952792 Msa0081420:five_prime_UTR
TCACCGCCTTGTTGCTGAAT+TGG 0.337037 1_2:+59952425 None:intergenic
TTGCAAAGTCCTTTAAGATT+AGG 0.337977 1_2:-59951549 Msa0081420:three_prime_UTR
AAGAATAAGCCGCCACCTTA+AGG 0.358276 1_2:+59952777 None:intergenic
TGGAGAAGGAGACTTGATTA+TGG 0.360172 1_2:-59952095 Msa0081420:CDS
ATCACAAGGGTTGATGCATT+CGG 0.373542 1_2:-59951780 Msa0081420:CDS
CTCAATTTGCTAGCTCATAA+TGG 0.373904 1_2:-59952326 Msa0081420:CDS
TCACAAGGGTTGATGCATTC+GGG 0.380840 1_2:-59951779 Msa0081420:CDS
TACTCTTTAGATTATGTTTG+AGG 0.388933 1_2:-59951662 Msa0081420:three_prime_UTR
AAGGAGACTTGATTATGGTT+GGG 0.404725 1_2:-59952090 Msa0081420:CDS
TACATGGAAGGCATTCCAAT+TGG 0.410476 1_2:-59952356 Msa0081420:CDS
TATGGTTGGGGATGTCCCTT+GGG 0.419336 1_2:-59952077 Msa0081420:CDS
AAATTGAGCTTTCTCCCAAT+TGG 0.430798 1_2:+59952341 None:intergenic
TTATGGTTGGGGATGTCCCT+TGG 0.433671 1_2:-59952078 Msa0081420:CDS
AGGAGACTTGATTATGGTTG+GGG 0.442249 1_2:-59952089 Msa0081420:CDS
TACAATTCCACAATGGGAAA+AGG 0.446881 1_2:-59952720 Msa0081420:exon
GAAGGAGACTTGATTATGGT+TGG 0.447956 1_2:-59952091 Msa0081420:CDS
AGAGAACAAAATCAAAATGA+AGG 0.451318 1_2:-59951615 Msa0081420:three_prime_UTR
AAATTTAAATATTCAAATGA+AGG 0.453913 1_2:+59951572 None:intergenic
CCTTCTTCTACTGTTGGAAG+AGG 0.459161 1_2:+59952680 None:intergenic
GGAGTGGTAATTACATAGAA+TGG 0.469288 1_2:+59952259 None:intergenic
ACATGGAAGGCATTCCAATT+GGG 0.472520 1_2:-59952355 Msa0081420:CDS
GGAAGAGGAGGAAGAAGAAG+TGG 0.473631 1_2:+59952695 None:intergenic
AAATTCTACAATTCCACAAT+GGG 0.475764 1_2:-59952726 Msa0081420:exon
GATAGAGGATGAAACATTAG+AGG 0.481392 1_2:+59952647 None:intergenic
CTTCTTTGTGAAGGTTTACA+TGG 0.492048 1_2:-59952372 Msa0081420:CDS
TCTAAAGAGTATCATCACTT+TGG 0.492372 1_2:+59951674 None:intergenic
CTCAATCCAAGGTTAAGATC+AGG 0.506435 1_2:+59952591 None:intergenic
CATGTAAACCTTCACAAAGA+AGG 0.519682 1_2:+59952373 None:intergenic
TTTGTGAAGGTTTACATGGA+AGG 0.523480 1_2:-59952368 Msa0081420:CDS
ATGTTTCCTTCTTCTACTGT+TGG 0.532208 1_2:+59952674 None:intergenic
TTTGTCATATTAGGGGACAT+TGG 0.532866 1_2:-59952451 Msa0081420:intron
TTATTTAAACCTTAAGGTGG+CGG 0.534814 1_2:-59952786 Msa0081420:five_prime_UTR
CCTCTTCCAACAGTAGAAGA+AGG 0.535777 1_2:-59952680 Msa0081420:CDS
TATGTTTGAGGACTAGCAAA+GGG 0.538199 1_2:-59951650 Msa0081420:three_prime_UTR
GTTAAGTAAACTTACTCCCA+AGG 0.550745 1_2:+59952061 None:intergenic
TCTTCTACTGTTGGAAGAGG+AGG 0.561746 1_2:+59952683 None:intergenic
CAACAGTGCCTTCTTTGTGA+AGG 0.564852 1_2:-59952381 Msa0081420:CDS
TAAATTTACATACCTTGAGG+TGG 0.569907 1_2:+59952536 None:intergenic
TAATAAAGAAAGAATTGAAG+AGG 0.571632 1_2:+59952806 None:intergenic
TCATAATGGCTATCATGAGT+TGG 0.572244 1_2:-59952312 Msa0081420:CDS
CATGTGTTAACTTATGAAGA+TGG 0.578380 1_2:-59952115 Msa0081420:CDS
ATCAACATATTGTTGAGTGG+TGG 0.579143 1_2:+59952563 None:intergenic
CAAATTCTACAATTCCACAA+TGG 0.580800 1_2:-59952727 Msa0081420:exon
TTATTATTTAAACCTTAAGG+TGG 0.581557 1_2:-59952789 Msa0081420:five_prime_UTR
ATGTTGATTATTCCACCTCA+AGG 0.585626 1_2:-59952548 Msa0081420:intron
TGTTCTTATCATCTGTGAAG+AGG 0.588185 1_2:-59951809 Msa0081420:CDS
TTAACTTATGAAGATGGAGA+AGG 0.590910 1_2:-59952109 Msa0081420:CDS
GAGAACAAAATCAAAATGAA+GGG 0.593834 1_2:-59951614 Msa0081420:three_prime_UTR
TTATGTTTGAGGACTAGCAA+AGG 0.602533 1_2:-59951651 Msa0081420:three_prime_UTR
TTAAGTAAACTTACTCCCAA+GGG 0.604202 1_2:+59952062 None:intergenic
TGAAGAGGTTGAAGATCACA+AGG 0.619538 1_2:-59951794 Msa0081420:CDS
TGGTGGAAATGCTCAATCCA+AGG 0.621212 1_2:+59952580 None:intergenic
TGGCAACCAATTCAGCAACA+AGG 0.686483 1_2:-59952431 Msa0081420:CDS
ATAATCAACATATTGTTGAG+TGG 0.703279 1_2:+59952560 None:intergenic
CAACCAATTCAGCAACAAGG+CGG 0.746050 1_2:-59952428 Msa0081420:CDS
GAAGAGGTTGAAGATCACAA+GGG 0.757105 1_2:-59951793 Msa0081420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAAATATTCAAATGA+AGG + chr1_2:59952776-59952795 None:intergenic 10.0%
!! AAATTTAAATATTCAAATGA+AGG + chr1_2:59952776-59952795 None:intergenic 10.0%
!! TCTTTATTATTTAAACCTTA+AGG - chr1_2:59951553-59951572 Msa0081420:three_prime_UTR 15.0%
!!! ATTTTTACTTCTTTTTTTGT+TGG - chr1_2:59952122-59952141 Msa0081420:CDS 15.0%
!! AATTTACTTAGAATTGAAAT+GGG + chr1_2:59952331-59952350 None:intergenic 15.0%
!! AAATTTACTTAGAATTGAAA+TGG + chr1_2:59952332-59952351 None:intergenic 15.0%
!! ATTTCAATTCTAAGTAAATT+TGG - chr1_2:59952331-59952350 Msa0081420:CDS 15.0%
!! CATCAATTAATTTACAAAAT+TGG + chr1_2:59952489-59952508 None:intergenic 15.0%
!!! ATTTTGTAAATTAATTGATG+TGG - chr1_2:59952489-59952508 Msa0081420:intron 15.0%
!! TCTTTATTATTTAAACCTTA+AGG - chr1_2:59951553-59951572 Msa0081420:three_prime_UTR 15.0%
!!! ATTTTTACTTCTTTTTTTGT+TGG - chr1_2:59952122-59952141 Msa0081420:CDS 15.0%
!! AATTTACTTAGAATTGAAAT+GGG + chr1_2:59952331-59952350 None:intergenic 15.0%
!! AAATTTACTTAGAATTGAAA+TGG + chr1_2:59952332-59952351 None:intergenic 15.0%
!! ATTTCAATTCTAAGTAAATT+TGG - chr1_2:59952331-59952350 Msa0081420:CDS 15.0%
!! CATCAATTAATTTACAAAAT+TGG + chr1_2:59952489-59952508 None:intergenic 15.0%
!!! ATTTTGTAAATTAATTGATG+TGG - chr1_2:59952489-59952508 Msa0081420:intron 15.0%
!! TAATAAAGAAAGAATTGAAG+AGG + chr1_2:59951542-59951561 None:intergenic 20.0%
!! TTATTATTTAAACCTTAAGG+TGG - chr1_2:59951556-59951575 Msa0081420:three_prime_UTR 20.0%
!!! TTTTAAATTTACATACCTTG+AGG + chr1_2:59951815-59951834 None:intergenic 20.0%
!!! ATTGTGTTATTTGTCATATT+AGG - chr1_2:59951885-59951904 Msa0081420:intron 20.0%
!!! TTGTGTTATTTGTCATATTA+GGG - chr1_2:59951886-59951905 Msa0081420:intron 20.0%
!! AAGTAAAAATGCATATATGA+GGG + chr1_2:59952112-59952131 None:intergenic 20.0%
!! AAGTAAATTTGGCATAATTA+TGG - chr1_2:59952342-59952361 Msa0081420:CDS 20.0%
!!! TACAATTTTTTTGGTTATGT+AGG - chr1_2:59952512-59952531 Msa0081420:intron 20.0%
!! TTCACTTCTCTAATTTAAAA+TGG + chr1_2:59952823-59952842 None:intergenic 20.0%
!! TAATAAAGAAAGAATTGAAG+AGG + chr1_2:59951542-59951561 None:intergenic 20.0%
!! TTATTATTTAAACCTTAAGG+TGG - chr1_2:59951556-59951575 Msa0081420:three_prime_UTR 20.0%
!!! TTTTAAATTTACATACCTTG+AGG + chr1_2:59951815-59951834 None:intergenic 20.0%
!!! ATTGTGTTATTTGTCATATT+AGG - chr1_2:59951885-59951904 Msa0081420:intron 20.0%
!!! TTGTGTTATTTGTCATATTA+GGG - chr1_2:59951886-59951905 Msa0081420:intron 20.0%
!! AAGTAAAAATGCATATATGA+GGG + chr1_2:59952112-59952131 None:intergenic 20.0%
!! AAGTAAATTTGGCATAATTA+TGG - chr1_2:59952342-59952361 Msa0081420:CDS 20.0%
!!! TACAATTTTTTTGGTTATGT+AGG - chr1_2:59952512-59952531 Msa0081420:intron 20.0%
!! TTCACTTCTCTAATTTAAAA+TGG + chr1_2:59952823-59952842 None:intergenic 20.0%
! AAATTCTACAATTCCACAAT+GGG - chr1_2:59951619-59951638 Msa0081420:three_prime_UTR 25.0%
! ATAATCAACATATTGTTGAG+TGG + chr1_2:59951788-59951807 None:intergenic 25.0%
!!! TGTGTTATTTGTCATATTAG+GGG - chr1_2:59951887-59951906 Msa0081420:intron 25.0%
! AGTAAAAATGCATATATGAG+GGG + chr1_2:59952111-59952130 None:intergenic 25.0%
! GAAGTAAAAATGCATATATG+AGG + chr1_2:59952113-59952132 None:intergenic 25.0%
!! TGTTGGTAAAATGAGTAATA+TGG - chr1_2:59952139-59952158 Msa0081420:CDS 25.0%
!!! TTGATGTGGTACAATTTTTT+TGG - chr1_2:59952503-59952522 Msa0081420:intron 25.0%
! TACTCTTTAGATTATGTTTG+AGG - chr1_2:59952683-59952702 Msa0081420:CDS 25.0%
! AGAGAACAAAATCAAAATGA+AGG - chr1_2:59952730-59952749 Msa0081420:exon 25.0%
! GAGAACAAAATCAAAATGAA+GGG - chr1_2:59952731-59952750 Msa0081420:five_prime_UTR 25.0%
! TAAAATGGACCTAATCTTAA+AGG + chr1_2:59952808-59952827 None:intergenic 25.0%
! AAATTCTACAATTCCACAAT+GGG - chr1_2:59951619-59951638 Msa0081420:three_prime_UTR 25.0%
! ATAATCAACATATTGTTGAG+TGG + chr1_2:59951788-59951807 None:intergenic 25.0%
!!! TGTGTTATTTGTCATATTAG+GGG - chr1_2:59951887-59951906 Msa0081420:intron 25.0%
! AGTAAAAATGCATATATGAG+GGG + chr1_2:59952111-59952130 None:intergenic 25.0%
! GAAGTAAAAATGCATATATG+AGG + chr1_2:59952113-59952132 None:intergenic 25.0%
!! TGTTGGTAAAATGAGTAATA+TGG - chr1_2:59952139-59952158 Msa0081420:CDS 25.0%
!!! TTGATGTGGTACAATTTTTT+TGG - chr1_2:59952503-59952522 Msa0081420:intron 25.0%
! TACTCTTTAGATTATGTTTG+AGG - chr1_2:59952683-59952702 Msa0081420:CDS 25.0%
! AGAGAACAAAATCAAAATGA+AGG - chr1_2:59952730-59952749 Msa0081420:exon 25.0%
! GAGAACAAAATCAAAATGAA+GGG - chr1_2:59952731-59952750 Msa0081420:five_prime_UTR 25.0%
! TAAAATGGACCTAATCTTAA+AGG + chr1_2:59952808-59952827 None:intergenic 25.0%
TTATTTAAACCTTAAGGTGG+CGG - chr1_2:59951559-59951578 Msa0081420:three_prime_UTR 30.0%
CATTGTGGAATTGTAGAATT+TGG + chr1_2:59951620-59951639 None:intergenic 30.0%
CAAATTCTACAATTCCACAA+TGG - chr1_2:59951618-59951637 Msa0081420:three_prime_UTR 30.0%
TAAATTTACATACCTTGAGG+TGG + chr1_2:59951812-59951831 None:intergenic 30.0%
GACACAAACATAGTAGTTTA+AGG + chr1_2:59951850-59951869 None:intergenic 30.0%
GTAAAAATGCATATATGAGG+GGG + chr1_2:59952110-59952129 None:intergenic 30.0%
CATGTGTTAACTTATGAAGA+TGG - chr1_2:59952230-59952249 Msa0081420:intron 30.0%
TTAACTTATGAAGATGGAGA+AGG - chr1_2:59952236-59952255 Msa0081420:intron 30.0%
TTAAGTAAACTTACTCCCAA+GGG + chr1_2:59952286-59952305 None:intergenic 30.0%
!! ATTTTGACACAAGTGACATT+GGG - chr1_2:59952377-59952396 Msa0081420:CDS 30.0%
!! TCTAAAGAGTATCATCACTT+TGG + chr1_2:59952674-59952693 None:intergenic 30.0%
!! TTGCAAAGTCCTTTAAGATT+AGG - chr1_2:59952796-59952815 Msa0081420:five_prime_UTR 30.0%
!!! TGAACAAAGAAGTGTTTTGA+AGG - chr1_2:59952839-59952858 Msa0081420:five_prime_UTR 30.0%
!!! GAACAAAGAAGTGTTTTGAA+GGG - chr1_2:59952840-59952859 Msa0081420:five_prime_UTR 30.0%
TTATTTAAACCTTAAGGTGG+CGG - chr1_2:59951559-59951578 Msa0081420:three_prime_UTR 30.0%
CATTGTGGAATTGTAGAATT+TGG + chr1_2:59951620-59951639 None:intergenic 30.0%
CAAATTCTACAATTCCACAA+TGG - chr1_2:59951618-59951637 Msa0081420:three_prime_UTR 30.0%
TAAATTTACATACCTTGAGG+TGG + chr1_2:59951812-59951831 None:intergenic 30.0%
GACACAAACATAGTAGTTTA+AGG + chr1_2:59951850-59951869 None:intergenic 30.0%
GTAAAAATGCATATATGAGG+GGG + chr1_2:59952110-59952129 None:intergenic 30.0%
CATGTGTTAACTTATGAAGA+TGG - chr1_2:59952230-59952249 Msa0081420:intron 30.0%
TTAACTTATGAAGATGGAGA+AGG - chr1_2:59952236-59952255 Msa0081420:intron 30.0%
TTAAGTAAACTTACTCCCAA+GGG + chr1_2:59952286-59952305 None:intergenic 30.0%
!! ATTTTGACACAAGTGACATT+GGG - chr1_2:59952377-59952396 Msa0081420:CDS 30.0%
!! TCTAAAGAGTATCATCACTT+TGG + chr1_2:59952674-59952693 None:intergenic 30.0%
!! TTGCAAAGTCCTTTAAGATT+AGG - chr1_2:59952796-59952815 Msa0081420:five_prime_UTR 30.0%
!!! TGAACAAAGAAGTGTTTTGA+AGG - chr1_2:59952839-59952858 Msa0081420:five_prime_UTR 30.0%
!!! GAACAAAGAAGTGTTTTGAA+GGG - chr1_2:59952840-59952859 Msa0081420:five_prime_UTR 30.0%
! AGCAAGAAAAAAGAGTATGC+TGG - chr1_2:59951490-59951509 Msa0081420:three_prime_UTR 35.0%
! GCAAGAAAAAAGAGTATGCT+GGG - chr1_2:59951491-59951510 Msa0081420:three_prime_UTR 35.0%
TACAATTCCACAATGGGAAA+AGG - chr1_2:59951625-59951644 Msa0081420:three_prime_UTR 35.0%
ATGTTTCCTTCTTCTACTGT+TGG + chr1_2:59951674-59951693 None:intergenic 35.0%
GATAGAGGATGAAACATTAG+AGG + chr1_2:59951701-59951720 None:intergenic 35.0%
!! AGGATTTTGATGTGTGATAG+AGG + chr1_2:59951716-59951735 None:intergenic 35.0%
GGAAAAAGATGTTGTTGATC+AGG + chr1_2:59951736-59951755 None:intergenic 35.0%
! CTTTTTCCTGATCTTAACCT+TGG - chr1_2:59951748-59951767 Msa0081420:three_prime_UTR 35.0%
ATCAACATATTGTTGAGTGG+TGG + chr1_2:59951785-59951804 None:intergenic 35.0%
! ATGTTGATTATTCCACCTCA+AGG - chr1_2:59951797-59951816 Msa0081420:CDS 35.0%
TTTGTCATATTAGGGGACAT+TGG - chr1_2:59951894-59951913 Msa0081420:intron 35.0%
CATGTAAACCTTCACAAAGA+AGG + chr1_2:59951975-59951994 None:intergenic 35.0%
CTTCTTTGTGAAGGTTTACA+TGG - chr1_2:59951973-59951992 Msa0081420:intron 35.0%
TTTGTGAAGGTTTACATGGA+AGG - chr1_2:59951977-59951996 Msa0081420:intron 35.0%
AAATTGAGCTTTCTCCCAAT+TGG + chr1_2:59952007-59952026 None:intergenic 35.0%
!! CTCAATTTGCTAGCTCATAA+TGG - chr1_2:59952019-59952038 Msa0081420:intron 35.0%
TCATAATGGCTATCATGAGT+TGG - chr1_2:59952033-59952052 Msa0081420:intron 35.0%
GGAGTGGTAATTACATAGAA+TGG + chr1_2:59952089-59952108 None:intergenic 35.0%
!!! TGTGACTTTGTTATTTTGCC+AGG - chr1_2:59952170-59952189 Msa0081420:intron 35.0%
!!! GTGACTTTGTTATTTTGCCA+GGG - chr1_2:59952171-59952190 Msa0081420:intron 35.0%
!!! TGACTTTGTTATTTTGCCAG+GGG - chr1_2:59952172-59952191 Msa0081420:intron 35.0%
AAGGAGACTTGATTATGGTT+GGG - chr1_2:59952255-59952274 Msa0081420:intron 35.0%
GTTAAGTAAACTTACTCCCA+AGG + chr1_2:59952287-59952306 None:intergenic 35.0%
!! GATTTTGACACAAGTGACAT+TGG - chr1_2:59952376-59952395 Msa0081420:CDS 35.0%
TGTTCTTATCATCTGTGAAG+AGG - chr1_2:59952536-59952555 Msa0081420:intron 35.0%
! TTATGTTTGAGGACTAGCAA+AGG - chr1_2:59952694-59952713 Msa0081420:CDS 35.0%
! TATGTTTGAGGACTAGCAAA+GGG - chr1_2:59952695-59952714 Msa0081420:CDS 35.0%
! AGCAAGAAAAAAGAGTATGC+TGG - chr1_2:59951490-59951509 Msa0081420:three_prime_UTR 35.0%
! GCAAGAAAAAAGAGTATGCT+GGG - chr1_2:59951491-59951510 Msa0081420:three_prime_UTR 35.0%
TACAATTCCACAATGGGAAA+AGG - chr1_2:59951625-59951644 Msa0081420:three_prime_UTR 35.0%
ATGTTTCCTTCTTCTACTGT+TGG + chr1_2:59951674-59951693 None:intergenic 35.0%
GATAGAGGATGAAACATTAG+AGG + chr1_2:59951701-59951720 None:intergenic 35.0%
!! AGGATTTTGATGTGTGATAG+AGG + chr1_2:59951716-59951735 None:intergenic 35.0%
GGAAAAAGATGTTGTTGATC+AGG + chr1_2:59951736-59951755 None:intergenic 35.0%
! CTTTTTCCTGATCTTAACCT+TGG - chr1_2:59951748-59951767 Msa0081420:three_prime_UTR 35.0%
ATCAACATATTGTTGAGTGG+TGG + chr1_2:59951785-59951804 None:intergenic 35.0%
! ATGTTGATTATTCCACCTCA+AGG - chr1_2:59951797-59951816 Msa0081420:CDS 35.0%
TTTGTCATATTAGGGGACAT+TGG - chr1_2:59951894-59951913 Msa0081420:intron 35.0%
CATGTAAACCTTCACAAAGA+AGG + chr1_2:59951975-59951994 None:intergenic 35.0%
CTTCTTTGTGAAGGTTTACA+TGG - chr1_2:59951973-59951992 Msa0081420:intron 35.0%
TTTGTGAAGGTTTACATGGA+AGG - chr1_2:59951977-59951996 Msa0081420:intron 35.0%
AAATTGAGCTTTCTCCCAAT+TGG + chr1_2:59952007-59952026 None:intergenic 35.0%
!! CTCAATTTGCTAGCTCATAA+TGG - chr1_2:59952019-59952038 Msa0081420:intron 35.0%
TCATAATGGCTATCATGAGT+TGG - chr1_2:59952033-59952052 Msa0081420:intron 35.0%
GGAGTGGTAATTACATAGAA+TGG + chr1_2:59952089-59952108 None:intergenic 35.0%
!!! TGTGACTTTGTTATTTTGCC+AGG - chr1_2:59952170-59952189 Msa0081420:intron 35.0%
!!! GTGACTTTGTTATTTTGCCA+GGG - chr1_2:59952171-59952190 Msa0081420:intron 35.0%
!!! TGACTTTGTTATTTTGCCAG+GGG - chr1_2:59952172-59952191 Msa0081420:intron 35.0%
AAGGAGACTTGATTATGGTT+GGG - chr1_2:59952255-59952274 Msa0081420:intron 35.0%
GTTAAGTAAACTTACTCCCA+AGG + chr1_2:59952287-59952306 None:intergenic 35.0%
!! GATTTTGACACAAGTGACAT+TGG - chr1_2:59952376-59952395 Msa0081420:CDS 35.0%
TGTTCTTATCATCTGTGAAG+AGG - chr1_2:59952536-59952555 Msa0081420:intron 35.0%
! TTATGTTTGAGGACTAGCAA+AGG - chr1_2:59952694-59952713 Msa0081420:CDS 35.0%
! TATGTTTGAGGACTAGCAAA+GGG - chr1_2:59952695-59952714 Msa0081420:CDS 35.0%
! AGAAAAAAGAGTATGCTGGG+TGG - chr1_2:59951494-59951513 Msa0081420:three_prime_UTR 40.0%
CTCAATCCAAGGTTAAGATC+AGG + chr1_2:59951757-59951776 None:intergenic 40.0%
!! TACATGGAAGGCATTCCAAT+TGG - chr1_2:59951989-59952008 Msa0081420:intron 40.0%
!! ACATGGAAGGCATTCCAATT+GGG - chr1_2:59951990-59952009 Msa0081420:intron 40.0%
!!! GACTTTGTTATTTTGCCAGG+GGG - chr1_2:59952173-59952192 Msa0081420:intron 40.0%
!!! ACTTTGTTATTTTGCCAGGG+GGG - chr1_2:59952174-59952193 Msa0081420:intron 40.0%
TGGAGAAGGAGACTTGATTA+TGG - chr1_2:59952250-59952269 Msa0081420:intron 40.0%
GAAGGAGACTTGATTATGGT+TGG - chr1_2:59952254-59952273 Msa0081420:intron 40.0%
AGGAGACTTGATTATGGTTG+GGG - chr1_2:59952256-59952275 Msa0081420:intron 40.0%
!!! GGCGACACTGATTTTGTTTA+AGG + chr1_2:59952405-59952424 None:intergenic 40.0%
GGTAACTGTGATTTCCAGTT+TGG + chr1_2:59952426-59952445 None:intergenic 40.0%
! TAACGTGTTGACGATGAGTA+CGG + chr1_2:59952447-59952466 None:intergenic 40.0%
TGAAGAGGTTGAAGATCACA+AGG - chr1_2:59952551-59952570 Msa0081420:CDS 40.0%
GAAGAGGTTGAAGATCACAA+GGG - chr1_2:59952552-59952571 Msa0081420:CDS 40.0%
!! ATCACAAGGGTTGATGCATT+CGG - chr1_2:59952565-59952584 Msa0081420:CDS 40.0%
! AGAAAAAAGAGTATGCTGGG+TGG - chr1_2:59951494-59951513 Msa0081420:three_prime_UTR 40.0%
CTCAATCCAAGGTTAAGATC+AGG + chr1_2:59951757-59951776 None:intergenic 40.0%
!! TACATGGAAGGCATTCCAAT+TGG - chr1_2:59951989-59952008 Msa0081420:intron 40.0%
!! ACATGGAAGGCATTCCAATT+GGG - chr1_2:59951990-59952009 Msa0081420:intron 40.0%
!!! GACTTTGTTATTTTGCCAGG+GGG - chr1_2:59952173-59952192 Msa0081420:intron 40.0%
!!! ACTTTGTTATTTTGCCAGGG+GGG - chr1_2:59952174-59952193 Msa0081420:intron 40.0%
TGGAGAAGGAGACTTGATTA+TGG - chr1_2:59952250-59952269 Msa0081420:intron 40.0%
GAAGGAGACTTGATTATGGT+TGG - chr1_2:59952254-59952273 Msa0081420:intron 40.0%
AGGAGACTTGATTATGGTTG+GGG - chr1_2:59952256-59952275 Msa0081420:intron 40.0%
!!! GGCGACACTGATTTTGTTTA+AGG + chr1_2:59952405-59952424 None:intergenic 40.0%
GGTAACTGTGATTTCCAGTT+TGG + chr1_2:59952426-59952445 None:intergenic 40.0%
! TAACGTGTTGACGATGAGTA+CGG + chr1_2:59952447-59952466 None:intergenic 40.0%
TGAAGAGGTTGAAGATCACA+AGG - chr1_2:59952551-59952570 Msa0081420:CDS 40.0%
GAAGAGGTTGAAGATCACAA+GGG - chr1_2:59952552-59952571 Msa0081420:CDS 40.0%
!! ATCACAAGGGTTGATGCATT+CGG - chr1_2:59952565-59952584 Msa0081420:CDS 40.0%
AAGAATAAGCCGCCACCTTA+AGG + chr1_2:59951571-59951590 None:intergenic 45.0%
! AGTGGAACCTTTTCCCATTG+TGG + chr1_2:59951635-59951654 None:intergenic 45.0%
TCTTCTACTGTTGGAAGAGG+AGG + chr1_2:59951665-59951684 None:intergenic 45.0%
CCTTCTTCTACTGTTGGAAG+AGG + chr1_2:59951668-59951687 None:intergenic 45.0%
CCTCTTCCAACAGTAGAAGA+AGG - chr1_2:59951665-59951684 Msa0081420:three_prime_UTR 45.0%
TGGTGGAAATGCTCAATCCA+AGG + chr1_2:59951768-59951787 None:intergenic 45.0%
TGGCAACCAATTCAGCAACA+AGG - chr1_2:59951914-59951933 Msa0081420:intron 45.0%
CAACCAATTCAGCAACAAGG+CGG - chr1_2:59951917-59951936 Msa0081420:intron 45.0%
CAACAGTGCCTTCTTTGTGA+AGG - chr1_2:59951964-59951983 Msa0081420:intron 45.0%
AATGCATATATGAGGGGGAG+TGG + chr1_2:59952105-59952124 None:intergenic 45.0%
CAAAATCAGTGTCGCCAAAC+TGG - chr1_2:59952409-59952428 Msa0081420:CDS 45.0%
!! TCACAAGGGTTGATGCATTC+GGG - chr1_2:59952566-59952585 Msa0081420:CDS 45.0%
AAGAATAAGCCGCCACCTTA+AGG + chr1_2:59951571-59951590 None:intergenic 45.0%
! AGTGGAACCTTTTCCCATTG+TGG + chr1_2:59951635-59951654 None:intergenic 45.0%
TCTTCTACTGTTGGAAGAGG+AGG + chr1_2:59951665-59951684 None:intergenic 45.0%
CCTTCTTCTACTGTTGGAAG+AGG + chr1_2:59951668-59951687 None:intergenic 45.0%
CCTCTTCCAACAGTAGAAGA+AGG - chr1_2:59951665-59951684 Msa0081420:three_prime_UTR 45.0%
TGGTGGAAATGCTCAATCCA+AGG + chr1_2:59951768-59951787 None:intergenic 45.0%
TGGCAACCAATTCAGCAACA+AGG - chr1_2:59951914-59951933 Msa0081420:intron 45.0%
CAACCAATTCAGCAACAAGG+CGG - chr1_2:59951917-59951936 Msa0081420:intron 45.0%
CAACAGTGCCTTCTTTGTGA+AGG - chr1_2:59951964-59951983 Msa0081420:intron 45.0%
AATGCATATATGAGGGGGAG+TGG + chr1_2:59952105-59952124 None:intergenic 45.0%
CAAAATCAGTGTCGCCAAAC+TGG - chr1_2:59952409-59952428 Msa0081420:CDS 45.0%
!! TCACAAGGGTTGATGCATTC+GGG - chr1_2:59952566-59952585 Msa0081420:CDS 45.0%
GGAAGAGGAGGAAGAAGAAG+TGG + chr1_2:59951653-59951672 None:intergenic 50.0%
! TCACCGCCTTGTTGCTGAAT+TGG + chr1_2:59951923-59951942 None:intergenic 50.0%
TTATGGTTGGGGATGTCCCT+TGG - chr1_2:59952267-59952286 Msa0081420:intron 50.0%
TATGGTTGGGGATGTCCCTT+GGG - chr1_2:59952268-59952287 Msa0081420:intron 50.0%
GGAAGAGGAGGAAGAAGAAG+TGG + chr1_2:59951653-59951672 None:intergenic 50.0%
! TCACCGCCTTGTTGCTGAAT+TGG + chr1_2:59951923-59951942 None:intergenic 50.0%
TTATGGTTGGGGATGTCCCT+TGG - chr1_2:59952267-59952286 Msa0081420:intron 50.0%
TATGGTTGGGGATGTCCCTT+GGG - chr1_2:59952268-59952287 Msa0081420:intron 50.0%
TTTGCCAGGGGGGACTGAAA+TGG - chr1_2:59952184-59952203 Msa0081420:intron 55.0%
TTTGCCAGGGGGGACTGAAA+TGG - chr1_2:59952184-59952203 Msa0081420:intron 55.0%
CCATCCATTTCAGTCCCCCC+TGG + chr1_2:59952191-59952210 None:intergenic 60.0%
CCAGGGGGGACTGAAATGGA+TGG - chr1_2:59952188-59952207 Msa0081420:intron 60.0%
CCATCCATTTCAGTCCCCCC+TGG + chr1_2:59952191-59952210 None:intergenic 60.0%
CCAGGGGGGACTGAAATGGA+TGG - chr1_2:59952188-59952207 Msa0081420:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 59951477 59952890 59951477 ID=Msa0081420;Name=Msa0081420
chr1_2 mRNA 59951477 59952890 59951477 ID=Msa0081420-mRNA-1;Parent=Msa0081420;Name=Msa0081420-mRNA-1;_AED=0.04;_eAED=0.04;_QI=160|1|1|1|1|1|4|296|176
chr1_2 exon 59951477 59951833 59951477 ID=Msa0081420-mRNA-1:exon:19499;Parent=Msa0081420-mRNA-1
chr1_2 exon 59952076 59952175 59952076 ID=Msa0081420-mRNA-1:exon:19498;Parent=Msa0081420-mRNA-1
chr1_2 exon 59952273 59952460 59952273 ID=Msa0081420-mRNA-1:exon:19497;Parent=Msa0081420-mRNA-1
chr1_2 exon 59952549 59952890 59952549 ID=Msa0081420-mRNA-1:exon:19496;Parent=Msa0081420-mRNA-1
chr1_2 five_prime_UTR 59952731 59952890 59952731 ID=Msa0081420-mRNA-1:five_prime_utr;Parent=Msa0081420-mRNA-1
chr1_2 CDS 59952549 59952730 59952549 ID=Msa0081420-mRNA-1:cds;Parent=Msa0081420-mRNA-1
chr1_2 CDS 59952273 59952460 59952273 ID=Msa0081420-mRNA-1:cds;Parent=Msa0081420-mRNA-1
chr1_2 CDS 59952076 59952175 59952076 ID=Msa0081420-mRNA-1:cds;Parent=Msa0081420-mRNA-1
chr1_2 CDS 59951773 59951833 59951773 ID=Msa0081420-mRNA-1:cds;Parent=Msa0081420-mRNA-1
chr1_2 three_prime_UTR 59951477 59951772 59951477 ID=Msa0081420-mRNA-1:three_prime_utr;Parent=Msa0081420-mRNA-1
Gene Sequence

>Msa0081420

ATGGGAAAAGGTTCCACTTCTTCTTCCTCCTCTTCCAACAGTAGAAGAAGGAAACATAATTCCTCTAATGTTTCATCCTCTATCACACATCAAAATCCTGATCAACAACATCTTTTTCCTGATCTTAACCTTGGATTGAGCATTTCCACCACTCAACAATATGTTGATTATTCCACCTCAAGGGGACATTGGCAACCAATTCAGCAACAAGGCGGTGAAGTAAATGATTGCAGTGATTACAACAGTGCCTTCTTTGTGAAGGTTTACATGGAAGGCATTCCAATTGGGAGAAAGCTCAATTTGCTAGCTCATAATGGCTATCATGAGTTGGTTAATACACTTGAACAAATGTTTGACACTACCATTCTATGGGGGACTGAAATGGATGGAGTGCAATCAGAGAGATGTCATGTGTTAACTTATGAAGATGGAGAAGGAGACTTGATTATGGTTGGGGATGTCCCTTGGGAGATGTTCTTATCATCTGTGAAGAGGTTGAAGATCACAAGGGTTGATGCATTCGGGTTTTAA
Protein sequence

>Msa0081420

MGKGSTSSSSSSNSRRRKHNSSNVSSSITHQNPDQQHLFPDLNLGLSISTTQQYVDYSTSRGHWQPIQQQGGEVNDCSDYNSAFFVKVYMEGIPIGRKLNLLAHNGYHELVNTLEQMFDTTILWGTEMDGVQSERCHVLTYEDGEGDLIMVGDVPWEMFLSSVKRLKITRVDAFGF*