AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0041870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041870 MtrunA17_Chr1g0207561 97.211 251 6 1 1 251 1 250 1.68e-177 488
Msa0041870 MtrunA17_Chr2g0305161 58.333 48 20 0 199 246 140 187 6.73e-14 68.2
Msa0041870 MtrunA17_Chr1g0169651 36.986 73 43 1 174 246 659 728 1.32e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041870 AT1G49850.1 41.860 258 128 4 1 246 1 248 1.07e-43 148
Msa0041870 AT2G15580.1 31.724 145 69 3 98 242 77 191 4.04e-15 72.0
Msa0041870 AT1G20823.1 40.659 91 45 3 164 247 70 158 8.67e-12 62.8
Msa0041870 AT1G76410.1 38.462 91 47 3 165 248 64 152 6.86e-11 60.1

Find 92 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAGTCCTGGTGGCTTTC+TGG 0.124154 1_1:-72444020 None:intergenic
ACTCTGCTTGTTTGGATCCT+TGG 0.223649 1_1:+72444191 Msa0041870:CDS
GCCACTGGTTCTCTCCTTCA+TGG 0.270330 1_1:-72444450 None:intergenic
TAAATTTCACTCTGCTTGTT+TGG 0.285473 1_1:+72444183 Msa0041870:CDS
CGTTGTAGTGGCGGCGGATA+CGG 0.287251 1_1:-72442001 None:intergenic
CTTTCACTTCCACTTGCTCT+TGG 0.328518 1_1:-72442422 None:intergenic
CTTCAAGATGCAAACAGTTC+AGG 0.330884 1_1:-72444051 None:intergenic
GTTGTAGTGGCGGCGGATAC+GGG 0.339096 1_1:-72442000 None:intergenic
CGATATCATTTCGTCCTAAT+CGG 0.375748 1_1:-72441899 None:intergenic
GCATTGTTTCATTCTATTGA+AGG 0.376853 1_1:-72444270 None:intergenic
ATCAGAATCCAAGGTACTTC+AGG 0.378655 1_1:+72444096 Msa0041870:CDS
ACAACAGGGTTGAAAATACT+TGG 0.387074 1_1:-72444386 None:intergenic
GGGAGCCTGTTGATAGAAAC+AGG 0.405614 1_1:+72442498 Msa0041870:CDS
TCGACTCACTCAATTACAAC+AGG 0.406077 1_1:-72444401 None:intergenic
AGGATAAAATATATGATGTA+AGG 0.408762 1_1:+72444475 Msa0041870:three_prime_UTR
TGAAACCTGTTTCTATCAAC+AGG 0.414171 1_1:-72442503 None:intergenic
GCGATACACTGCGACTCTTT+CGG 0.415466 1_1:-72441826 None:intergenic
ATTTATGTCCACATTGCAAA+TGG 0.420763 1_1:-72444165 None:intergenic
CGCGCAAGTCTCACATCTAC+TGG 0.427263 1_1:-72442449 None:intergenic
TACTGACTTGACTTCTCAAA+TGG 0.434814 1_1:+72443970 Msa0041870:CDS
GTTCCTCTCACACTTTCTCC+AGG 0.437499 1_1:-72442398 None:intergenic
CGGTGGTGATGATGGAAATC+TGG 0.449253 1_1:-72441948 None:intergenic
CGTGTATCGGAAAGATTTGA+TGG 0.453526 1_1:+72442335 Msa0041870:CDS
TGGTTTGTTCATGCAAGAGC+TGG 0.460636 1_1:-72444000 None:intergenic
AGGGCCTCCTGAGTGAGTCC+TGG 0.461922 1_1:-72444031 None:intergenic
CCAAGGTACTTCAGGATTGT+GGG 0.469017 1_1:+72444104 Msa0041870:CDS
CAAGGATGCTGCAACGGAGC+TGG 0.470411 1_1:+72444331 Msa0041870:three_prime_UTR
TTCAAGATGCAAACAGTTCA+GGG 0.472566 1_1:-72444050 None:intergenic
GACTCCTTCAGAGGTTGAGA+GGG 0.475373 1_1:+72442477 Msa0041870:CDS
AAGGAGAGAACCAGTGGCAG+AGG 0.476716 1_1:+72444455 Msa0041870:three_prime_UTR
AATGAAGGCGGCGTTGAACC+TGG 0.477109 1_1:+72442380 Msa0041870:CDS
AGCAAGGCACGTATTGGCTC+CGG 0.477346 1_1:-72444426 None:intergenic
CCACTGGTTCTCTCCTTCAT+GGG 0.481486 1_1:-72444449 None:intergenic
CGTGAAGGCGGTGGTGATGA+TGG 0.483202 1_1:-72441956 None:intergenic
AGGGTACGTGGTGACGGAGG+CGG 0.483634 1_1:-72442028 None:intergenic
TCCAAGGTACTTCAGGATTG+TGG 0.484189 1_1:+72444103 Msa0041870:CDS
CATGGGAGCAAGGCACGTAT+TGG 0.486115 1_1:-72444432 None:intergenic
TCAGGATTGTGGGATATGCT+TGG 0.490906 1_1:+72444114 Msa0041870:CDS
CTTCAGAACTATTCCACATC+AGG 0.492422 1_1:+72441871 Msa0041870:CDS
GTAATTGAGTGAGTCGAAAC+CGG 0.501618 1_1:+72444407 Msa0041870:three_prime_UTR
GATATGCTTGGAATCTTTCG+TGG 0.503539 1_1:+72444126 Msa0041870:CDS
ACTATACATCTTCGACAGTA+AGG 0.510903 1_1:-72444235 None:intergenic
TTCTCTCCTTCATGGGAGCA+AGG 0.517548 1_1:-72444442 None:intergenic
CCCACAATCCTGAAGTACCT+TGG 0.517671 1_1:-72444104 None:intergenic
CACATCAGGAGACACCGATT+AGG 0.521300 1_1:+72441885 Msa0041870:CDS
TGCTTGGAATCTTTCGTGGA+TGG 0.531615 1_1:+72444130 Msa0041870:CDS
GTAATTAAAGAGTCCTCAGT+TGG 0.533031 1_1:-72443950 None:intergenic
CGTCGGAGTCGTGAAGGCGG+TGG 0.536072 1_1:-72441965 None:intergenic
GTACGTGGTGACGGAGGCGG+CGG 0.538987 1_1:-72442025 None:intergenic
AGACTCCTTCAGAGGTTGAG+AGG 0.543708 1_1:+72442476 Msa0041870:CDS
GATCCTTGGATTCGCAGTTG+TGG 0.545640 1_1:+72444205 Msa0041870:CDS
CTAATCGGTGTCTCCTGATG+TGG 0.556727 1_1:-72441884 None:intergenic
GCCTCCTGAGTGAGTCCTGG+TGG 0.559542 1_1:-72444028 None:intergenic
GCTCCCCTCTCAACCTCTGA+AGG 0.559895 1_1:-72442481 None:intergenic
CGCGAGAAAGACTCCTTCAG+AGG 0.559940 1_1:+72442468 Msa0041870:CDS
AGAGGAACTCCAAGAGCAAG+TGG 0.560537 1_1:+72442413 Msa0041870:CDS
GGAGGCGGCGGACGTTGTAG+TGG 0.563566 1_1:-72442013 None:intergenic
GGCGGACGTTGTAGTGGCGG+CGG 0.567742 1_1:-72442007 None:intergenic
CGTCGATCTCTAGTAAATGA+AGG 0.571288 1_1:+72442365 Msa0041870:CDS
GGCGGCGGACGTTGTAGTGG+CGG 0.571784 1_1:-72442010 None:intergenic
AATCTGGAGAAGGTGAAGAG+TGG 0.578304 1_1:-72441932 None:intergenic
TCTCCACAACTGCGAATCCA+AGG 0.579205 1_1:-72444208 None:intergenic
ACAGGAAGGGTACGTGGTGA+CGG 0.583288 1_1:-72442034 None:intergenic
TTTACAGTATCGATTCTTCG+TGG 0.585056 1_1:+72441765 Msa0041870:five_prime_UTR
TACGTGCCTTGCTCCCATGA+AGG 0.587476 1_1:+72444436 Msa0041870:three_prime_UTR
CAACAGGGTTGAAAATACTT+GGG 0.588268 1_1:-72444385 None:intergenic
CTGTGTCAAGGATGCTGCAA+CGG 0.589797 1_1:+72444325 Msa0041870:three_prime_UTR
GGATAAAATATATGATGTAA+GGG 0.591614 1_1:+72444476 Msa0041870:three_prime_UTR
CATCGTCGTCGGAGTCGTGA+AGG 0.593058 1_1:-72441971 None:intergenic
TATCGGAAAGATTTGATGGA+AGG 0.593860 1_1:+72442339 Msa0041870:CDS
TGATAGAGATTCAGTGGACG+AGG 0.594106 1_1:+72443898 Msa0041870:CDS
TGATACGGAATCAGAATCCA+AGG 0.596863 1_1:+72444087 Msa0041870:CDS
AAAGCCACCAGGACTCACTC+AGG 0.599767 1_1:+72444024 Msa0041870:CDS
ACTTGACTTCTCAAATGGCA+AGG 0.603031 1_1:+72443975 Msa0041870:CDS
TGTTTGTTAGGAGCGTGTAT+CGG 0.603047 1_1:+72442322 Msa0041870:intron
GCAAAGTGATAGAGATTCAG+TGG 0.604810 1_1:+72443892 Msa0041870:CDS
CGACTCACTCAATTACAACA+GGG 0.616920 1_1:-72444400 None:intergenic
CGTCGTCGGAGTCGTGAAGG+CGG 0.629570 1_1:-72441968 None:intergenic
GGAAGGGTACGTGGTGACGG+AGG 0.634609 1_1:-72442031 None:intergenic
GAACAAACCAGAAAGCCACC+AGG 0.634998 1_1:+72444013 Msa0041870:CDS
TTGTAGTGGCGGCGGATACG+GGG 0.635138 1_1:-72441999 None:intergenic
ACGGGGAGAATCATCGTCGT+CGG 0.635388 1_1:-72441982 None:intergenic
GAACCTGGAGAAAGTGTGAG+AGG 0.649571 1_1:+72442395 Msa0041870:CDS
CCCATGAAGGAGAGAACCAG+TGG 0.652765 1_1:+72444449 Msa0041870:three_prime_UTR
AAACTGATCCATTTGCAATG+TGG 0.665356 1_1:+72444157 Msa0041870:CDS
TGATGATGGAAATCTGGAGA+AGG 0.668735 1_1:-72441942 None:intergenic
TACACTGCGACTCTTTCGGT+CGG 0.670884 1_1:-72441822 None:intergenic
ATCAAGTGAAGTTCCAACTG+AGG 0.674729 1_1:+72443937 Msa0041870:CDS
AAGAATCGATACTGTAAATG+CGG 0.682308 1_1:-72441760 None:intergenic
ACTCCTTCAGAGGTTGAGAG+GGG 0.685216 1_1:+72442478 Msa0041870:CDS
GCCACCAGGACTCACTCAGG+AGG 0.692200 1_1:+72444027 Msa0041870:CDS
CGATCTCTAGTAAATGAAGG+CGG 0.728434 1_1:+72442368 Msa0041870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTATTTTATTACAATTC+TGG + chr1_1:72442152-72442171 Msa0041870:intron 10.0%
!!! AAGAAAATAGAAATAGTTTT+TGG - chr1_1:72442549-72442568 None:intergenic 15.0%
!!! AAGCAAGAAATTATATTTTT+TGG - chr1_1:72443349-72443368 None:intergenic 15.0%
!!! TTTTCTGATTTCTAATTATT+GGG + chr1_1:72443576-72443595 Msa0041870:intron 15.0%
!! ACAAGTTCTATAAAAAAAAA+AGG - chr1_1:72443641-72443660 None:intergenic 15.0%
!! ATGATTAATGAGATAATTGA+TGG + chr1_1:72442197-72442216 Msa0041870:intron 20.0%
!!! TAATGTATTTTGTGTTTGTT+AGG + chr1_1:72442310-72442329 Msa0041870:intron 20.0%
!!! TTGCTACTTATATAATTGTT+AGG + chr1_1:72442624-72442643 Msa0041870:intron 20.0%
!! TACTTATATAATTGTTAGGT+TGG + chr1_1:72442628-72442647 Msa0041870:intron 20.0%
!! ACTTATATAATTGTTAGGTT+GGG + chr1_1:72442629-72442648 Msa0041870:intron 20.0%
!!! GACAATTTTAATTACAATCA+CGG - chr1_1:72442710-72442729 None:intergenic 20.0%
!! TGTAATTAAAATTGTCTCTA+AGG + chr1_1:72442714-72442733 Msa0041870:intron 20.0%
!! ATCGTGAAAGAAAAAAAAAA+AGG - chr1_1:72443254-72443273 None:intergenic 20.0%
!! TACATTGATCTATATTTAAG+TGG - chr1_1:72443308-72443327 None:intergenic 20.0%
!!! CAAGAAATTATATTTTTTGG+AGG - chr1_1:72443346-72443365 None:intergenic 20.0%
!! AAAATATAATTTCTTGCTTG+TGG + chr1_1:72443350-72443369 Msa0041870:intron 20.0%
!!! CTTTTCTGATTTCTAATTAT+TGG + chr1_1:72443575-72443594 Msa0041870:intron 20.0%
!! TTTCTGATTTCTAATTATTG+GGG + chr1_1:72443577-72443596 Msa0041870:intron 20.0%
!! TAAACTCAACAAAATTTGAA+GGG - chr1_1:72443608-72443627 None:intergenic 20.0%
!! ATAAACTCAACAAAATTTGA+AGG - chr1_1:72443609-72443628 None:intergenic 20.0%
!! AGGATAAAATATATGATGTA+AGG + chr1_1:72444475-72444494 Msa0041870:three_prime_UTR 20.0%
!! GGATAAAATATATGATGTAA+GGG + chr1_1:72444476-72444495 Msa0041870:three_prime_UTR 20.0%
!!! ACTTTTGATTCATTCTTACA+AGG + chr1_1:72441799-72441818 Msa0041870:five_prime_UTR 25.0%
! TATGTGTAGGAAAATAAAAC+AGG - chr1_1:72442055-72442074 None:intergenic 25.0%
! AGTAGAAGATGATGAAAATA+GGG - chr1_1:72442097-72442116 None:intergenic 25.0%
! TATTCAAATTCATAATTGCC+AGG + chr1_1:72442120-72442139 Msa0041870:intron 25.0%
!! ACTAACTAGTCTAACTTAAA+TGG + chr1_1:72442264-72442283 Msa0041870:intron 25.0%
! AGATTCTATTGAAAATTGCA+TGG + chr1_1:72443105-72443124 Msa0041870:intron 25.0%
! AGAGACATAATTGTAACATT+AGG + chr1_1:72443139-72443158 Msa0041870:intron 25.0%
!!! ATTAAGTTTTGGTCAGTATT+AGG + chr1_1:72443449-72443468 Msa0041870:intron 25.0%
!!! TTAAGTTTTGGTCAGTATTA+GGG + chr1_1:72443450-72443469 Msa0041870:intron 25.0%
!! CAAATTTTGTTGAGTTTATC+AGG + chr1_1:72443610-72443629 Msa0041870:intron 25.0%
!!! TAAGTTGATTGTTCTGATTT+AGG + chr1_1:72443738-72443757 Msa0041870:intron 25.0%
!! ATTTTCTTCTGCATCATTTT+TGG + chr1_1:72443789-72443808 Msa0041870:intron 25.0%
!!! TTTTGGTTGTTTATTCTTGA+AGG + chr1_1:72443806-72443825 Msa0041870:intron 25.0%
AAGAATCGATACTGTAAATG+CGG - chr1_1:72441763-72441782 None:intergenic 30.0%
GTAGGAAAATAAAACAGGAA+GGG - chr1_1:72442050-72442069 None:intergenic 30.0%
TGTAGGAAAATAAAACAGGA+AGG - chr1_1:72442051-72442070 None:intergenic 30.0%
TTGAACATATGTGTATGTGT+AGG - chr1_1:72442068-72442087 None:intergenic 30.0%
CAGTAGAAGATGATGAAAAT+AGG - chr1_1:72442098-72442117 None:intergenic 30.0%
AAATAATTACAGTCAGAACC+TGG - chr1_1:72442141-72442160 None:intergenic 30.0%
! ATTTTACTCCTCGAAATTGT+AGG + chr1_1:72442796-72442815 Msa0041870:intron 30.0%
! TTTTACTCCTCGAAATTGTA+GGG + chr1_1:72442797-72442816 Msa0041870:intron 30.0%
TACAAATGAGTGAGTAACAA+GGG - chr1_1:72442906-72442925 None:intergenic 30.0%
ATACAAATGAGTGAGTAACA+AGG - chr1_1:72442907-72442926 None:intergenic 30.0%
TTTCCTTGTTGGTATTTCAT+TGG + chr1_1:72442942-72442961 Msa0041870:intron 30.0%
! GCTTTTGATACTTCCATTTA+GGG + chr1_1:72442964-72442983 Msa0041870:intron 30.0%
TAACATTAGGTGATATTCTG+CGG + chr1_1:72443152-72443171 Msa0041870:intron 30.0%
CTTCATCTTATTAGAGCATA+TGG + chr1_1:72443424-72443443 Msa0041870:intron 30.0%
! AGCATATGGAGATTAAGTTT+TGG + chr1_1:72443438-72443457 Msa0041870:intron 30.0%
ATTGTGCATATTTATTGCTC+TGG + chr1_1:72443483-72443502 Msa0041870:intron 30.0%
!!! ACTGGTCTTTTTTCATAGTT+CGG + chr1_1:72443705-72443724 Msa0041870:intron 30.0%
TTGATTGTTCTGATTTAGGA+AGG + chr1_1:72443742-72443761 Msa0041870:intron 30.0%
AAAGCTTCAAACTGAATCAA+AGG - chr1_1:72443844-72443863 None:intergenic 30.0%
!!! TTGAAGCTTTACCTTTTTTG+TGG + chr1_1:72443854-72443873 Msa0041870:intron 30.0%
!!! TGAAGCTTTACCTTTTTTGT+GGG + chr1_1:72443855-72443874 Msa0041870:intron 30.0%
!!! AGTTTTTAGCAGCATTGATA+CGG + chr1_1:72444072-72444091 Msa0041870:CDS 30.0%
ATTTATGTCCACATTGCAAA+TGG - chr1_1:72444168-72444187 None:intergenic 30.0%
TAAATTTCACTCTGCTTGTT+TGG + chr1_1:72444183-72444202 Msa0041870:CDS 30.0%
GCATTGTTTCATTCTATTGA+AGG - chr1_1:72444273-72444292 None:intergenic 30.0%
!!! TTTATTTTGTGACTGTGTCA+AGG + chr1_1:72444313-72444332 Msa0041870:three_prime_UTR 30.0%
! GTTTTGTATCTATCTATGAC+AGG + chr1_1:72444502-72444521 Msa0041870:three_prime_UTR 30.0%
!! TTTACAGTATCGATTCTTCG+TGG + chr1_1:72441765-72441784 Msa0041870:five_prime_UTR 35.0%
CGATATCATTTCGTCCTAAT+CGG - chr1_1:72441902-72441921 None:intergenic 35.0%
TATCGGAAAGATTTGATGGA+AGG + chr1_1:72442339-72442358 Msa0041870:CDS 35.0%
!! TGAAACCTGTTTCTATCAAC+AGG - chr1_1:72442506-72442525 None:intergenic 35.0%
TTTACTCCTCGAAATTGTAG+GGG + chr1_1:72442798-72442817 Msa0041870:intron 35.0%
GACATACAAACAGCAATTGA+CGG - chr1_1:72442850-72442869 None:intergenic 35.0%
GTTACTCACTCATTTGTATG+TGG + chr1_1:72442908-72442927 Msa0041870:intron 35.0%
AAGCCAATGAAATACCAACA+AGG - chr1_1:72442948-72442967 None:intergenic 35.0%
! GGCTTTTGATACTTCCATTT+AGG + chr1_1:72442963-72442982 Msa0041870:intron 35.0%
! TTTCTGCGTTAGTGCAATTT+TGG - chr1_1:72443206-72443225 None:intergenic 35.0%
! AAAAGGATTAGCAACACTTC+TGG - chr1_1:72443237-72443256 None:intergenic 35.0%
TCTTTCACGATCTTGTATGT+TGG + chr1_1:72443263-72443282 Msa0041870:intron 35.0%
!!! TTTTTTGGAGGATTACGTCA+AGG - chr1_1:72443334-72443353 None:intergenic 35.0%
AATTTCTTGCTTGTGGAACT+TGG + chr1_1:72443357-72443376 Msa0041870:intron 35.0%
ATTTCTTGCTTGTGGAACTT+GGG + chr1_1:72443358-72443377 Msa0041870:intron 35.0%
!! AGAACTTGTCGATTTACTCA+AGG + chr1_1:72443652-72443671 Msa0041870:intron 35.0%
AGCTCTTCTAATAATCAGAC+TGG + chr1_1:72443687-72443706 Msa0041870:intron 35.0%
AATTCAATGTCAGATGCACA+GGG - chr1_1:72443772-72443791 None:intergenic 35.0%
AAATTCAATGTCAGATGCAC+AGG - chr1_1:72443773-72443792 None:intergenic 35.0%
TCAAACTGAATCAAAGGCAA+TGG - chr1_1:72443838-72443857 None:intergenic 35.0%
!!! CTTTACCTTTTTTGTGGGAT+TGG + chr1_1:72443860-72443879 Msa0041870:intron 35.0%
GTAATTAAAGAGTCCTCAGT+TGG - chr1_1:72443953-72443972 None:intergenic 35.0%
!! TACTGACTTGACTTCTCAAA+TGG + chr1_1:72443970-72443989 Msa0041870:CDS 35.0%
TTCAAGATGCAAACAGTTCA+GGG - chr1_1:72444053-72444072 None:intergenic 35.0%
AAACTGATCCATTTGCAATG+TGG + chr1_1:72444157-72444176 Msa0041870:CDS 35.0%
ACTATACATCTTCGACAGTA+AGG - chr1_1:72444238-72444257 None:intergenic 35.0%
CAACAGGGTTGAAAATACTT+GGG - chr1_1:72444388-72444407 None:intergenic 35.0%
ACAACAGGGTTGAAAATACT+TGG - chr1_1:72444389-72444408 None:intergenic 35.0%
! TATATTTTATCCTCTGCCAC+TGG - chr1_1:72444468-72444487 None:intergenic 35.0%
CTTCAGAACTATTCCACATC+AGG + chr1_1:72441871-72441890 Msa0041870:CDS 40.0%
!! TGATGATGGAAATCTGGAGA+AGG - chr1_1:72441945-72441964 None:intergenic 40.0%
! AATAAAACAGGAAGGGTACG+TGG - chr1_1:72442043-72442062 None:intergenic 40.0%
! TGTTTGTTAGGAGCGTGTAT+CGG + chr1_1:72442322-72442341 Msa0041870:intron 40.0%
CGTGTATCGGAAAGATTTGA+TGG + chr1_1:72442335-72442354 Msa0041870:CDS 40.0%
CGTCGATCTCTAGTAAATGA+AGG + chr1_1:72442365-72442384 Msa0041870:CDS 40.0%
CGATCTCTAGTAAATGAAGG+CGG + chr1_1:72442368-72442387 Msa0041870:CDS 40.0%
TTTGTATGTCACTGAGTCAC+TGG + chr1_1:72442860-72442879 Msa0041870:intron 40.0%
TTGACCAATTTCAACCGACA+AGG + chr1_1:72443050-72443069 Msa0041870:intron 40.0%
TTCTCATGATGCATCCTTGT+CGG - chr1_1:72443067-72443086 None:intergenic 40.0%
AATTGCACTAACGCAGAAAC+AGG + chr1_1:72443207-72443226 Msa0041870:intron 40.0%
!! GTTTTGGTCAGTATTAGGGA+CGG + chr1_1:72443454-72443473 Msa0041870:intron 40.0%
ATTCAATGTCAGATGCACAG+GGG - chr1_1:72443771-72443790 None:intergenic 40.0%
GGAAACCAATCCCACAAAAA+AGG - chr1_1:72443868-72443887 None:intergenic 40.0%
AATCTCTATCACTTTGCCTG+AGG - chr1_1:72443889-72443908 None:intergenic 40.0%
GCAAAGTGATAGAGATTCAG+TGG + chr1_1:72443892-72443911 Msa0041870:CDS 40.0%
ATCAAGTGAAGTTCCAACTG+AGG + chr1_1:72443937-72443956 Msa0041870:CDS 40.0%
ACTTGACTTCTCAAATGGCA+AGG + chr1_1:72443975-72443994 Msa0041870:CDS 40.0%
CTTCAAGATGCAAACAGTTC+AGG - chr1_1:72444054-72444073 None:intergenic 40.0%
TGATACGGAATCAGAATCCA+AGG + chr1_1:72444087-72444106 Msa0041870:CDS 40.0%
! ATCAGAATCCAAGGTACTTC+AGG + chr1_1:72444096-72444115 Msa0041870:CDS 40.0%
GATATGCTTGGAATCTTTCG+TGG + chr1_1:72444126-72444145 Msa0041870:CDS 40.0%
! GCTGGCATTTTATAGTGATG+TGG + chr1_1:72444349-72444368 Msa0041870:three_prime_UTR 40.0%
CGACTCACTCAATTACAACA+GGG - chr1_1:72444403-72444422 None:intergenic 40.0%
TCGACTCACTCAATTACAAC+AGG - chr1_1:72444404-72444423 None:intergenic 40.0%
GTAATTGAGTGAGTCGAAAC+CGG + chr1_1:72444407-72444426 Msa0041870:three_prime_UTR 40.0%
AATCTGGAGAAGGTGAAGAG+TGG - chr1_1:72441935-72441954 None:intergenic 45.0%
CTTTCACTTCCACTTGCTCT+TGG - chr1_1:72442425-72442444 None:intergenic 45.0%
AGTGAGTAACAAGGGACCAA+GGG - chr1_1:72442898-72442917 None:intergenic 45.0%
!! ACAGCTGCTGATTTCCTTGT+TGG + chr1_1:72442931-72442950 Msa0041870:intron 45.0%
! GCATCCTTGTCGGTTGAAAT+TGG - chr1_1:72443057-72443076 None:intergenic 45.0%
TGAATCAAAGGCAATGGCAG+AGG - chr1_1:72443832-72443851 None:intergenic 45.0%
!! TTTGTGGGATTGGTTTCCTC+AGG + chr1_1:72443870-72443889 Msa0041870:intron 45.0%
TGATAGAGATTCAGTGGACG+AGG + chr1_1:72443898-72443917 Msa0041870:CDS 45.0%
!! TGGTTTGTTCATGCAAGAGC+TGG - chr1_1:72444003-72444022 None:intergenic 45.0%
! TCCAAGGTACTTCAGGATTG+TGG + chr1_1:72444103-72444122 Msa0041870:CDS 45.0%
! CCAAGGTACTTCAGGATTGT+GGG + chr1_1:72444104-72444123 Msa0041870:CDS 45.0%
TCAGGATTGTGGGATATGCT+TGG + chr1_1:72444114-72444133 Msa0041870:CDS 45.0%
TGCTTGGAATCTTTCGTGGA+TGG + chr1_1:72444130-72444149 Msa0041870:CDS 45.0%
ACTCTGCTTGTTTGGATCCT+TGG + chr1_1:72444191-72444210 Msa0041870:CDS 45.0%
TACACTGCGACTCTTTCGGT+CGG - chr1_1:72441825-72441844 None:intergenic 50.0%
GCGATACACTGCGACTCTTT+CGG - chr1_1:72441829-72441848 None:intergenic 50.0%
! CTAATCGGTGTCTCCTGATG+TGG - chr1_1:72441887-72441906 None:intergenic 50.0%
! CACATCAGGAGACACCGATT+AGG + chr1_1:72441885-72441904 Msa0041870:CDS 50.0%
!! CGGTGGTGATGATGGAAATC+TGG - chr1_1:72441951-72441970 None:intergenic 50.0%
! GAACCTGGAGAAAGTGTGAG+AGG + chr1_1:72442395-72442414 Msa0041870:CDS 50.0%
GTTCCTCTCACACTTTCTCC+AGG - chr1_1:72442401-72442420 None:intergenic 50.0%
AGAGGAACTCCAAGAGCAAG+TGG + chr1_1:72442413-72442432 Msa0041870:CDS 50.0%
AGACTCCTTCAGAGGTTGAG+AGG + chr1_1:72442476-72442495 Msa0041870:CDS 50.0%
GACTCCTTCAGAGGTTGAGA+GGG + chr1_1:72442477-72442496 Msa0041870:CDS 50.0%
ACTCCTTCAGAGGTTGAGAG+GGG + chr1_1:72442478-72442497 Msa0041870:CDS 50.0%
! GGGAGCCTGTTGATAGAAAC+AGG + chr1_1:72442498-72442517 Msa0041870:CDS 50.0%
GAGTGAGTAACAAGGGACCA+AGG - chr1_1:72442899-72442918 None:intergenic 50.0%
AGCACAGAGTGAGCCCTAAA+TGG - chr1_1:72442980-72442999 None:intergenic 50.0%
TATGTTGGTCCACTGTGCTG+TGG + chr1_1:72443278-72443297 Msa0041870:intron 50.0%
GAACAAACCAGAAAGCCACC+AGG + chr1_1:72444013-72444032 Msa0041870:CDS 50.0%
! CCCACAATCCTGAAGTACCT+TGG - chr1_1:72444107-72444126 None:intergenic 50.0%
GATCCTTGGATTCGCAGTTG+TGG + chr1_1:72444205-72444224 Msa0041870:CDS 50.0%
TCTCCACAACTGCGAATCCA+AGG - chr1_1:72444211-72444230 None:intergenic 50.0%
CTGTGTCAAGGATGCTGCAA+CGG + chr1_1:72444325-72444344 Msa0041870:three_prime_UTR 50.0%
TTCTCTCCTTCATGGGAGCA+AGG - chr1_1:72444445-72444464 None:intergenic 50.0%
!! CCACTGGTTCTCTCCTTCAT+GGG - chr1_1:72444452-72444471 None:intergenic 50.0%
ACGGGGAGAATCATCGTCGT+CGG - chr1_1:72441985-72442004 None:intergenic 55.0%
! ACAGGAAGGGTACGTGGTGA+CGG - chr1_1:72442037-72442056 None:intergenic 55.0%
! AATGAAGGCGGCGTTGAACC+TGG + chr1_1:72442380-72442399 Msa0041870:CDS 55.0%
CGCGCAAGTCTCACATCTAC+TGG - chr1_1:72442452-72442471 None:intergenic 55.0%
CGCGAGAAAGACTCCTTCAG+AGG + chr1_1:72442468-72442487 Msa0041870:CDS 55.0%
GCTCGACCCCTACAATTTCG+AGG - chr1_1:72442807-72442826 None:intergenic 55.0%
CTGGCTGACACAGATTCCCT+TGG + chr1_1:72442879-72442898 Msa0041870:intron 55.0%
AGTGGTTGTCCACAGCACAG+TGG - chr1_1:72443290-72443309 None:intergenic 55.0%
AGTGAGTCCTGGTGGCTTTC+TGG - chr1_1:72444023-72444042 None:intergenic 55.0%
AAAGCCACCAGGACTCACTC+AGG + chr1_1:72444024-72444043 Msa0041870:CDS 55.0%
!! AGCAAGGCACGTATTGGCTC+CGG - chr1_1:72444429-72444448 None:intergenic 55.0%
CATGGGAGCAAGGCACGTAT+TGG - chr1_1:72444435-72444454 None:intergenic 55.0%
TACGTGCCTTGCTCCCATGA+AGG + chr1_1:72444436-72444455 Msa0041870:three_prime_UTR 55.0%
CCCATGAAGGAGAGAACCAG+TGG + chr1_1:72444449-72444468 Msa0041870:three_prime_UTR 55.0%
!! GCCACTGGTTCTCTCCTTCA+TGG - chr1_1:72444453-72444472 None:intergenic 55.0%
AAGGAGAGAACCAGTGGCAG+AGG + chr1_1:72444455-72444474 Msa0041870:three_prime_UTR 55.0%
! CGTGAAGGCGGTGGTGATGA+TGG - chr1_1:72441959-72441978 None:intergenic 60.0%
CATCGTCGTCGGAGTCGTGA+AGG - chr1_1:72441974-72441993 None:intergenic 60.0%
TTGTAGTGGCGGCGGATACG+GGG - chr1_1:72442002-72442021 None:intergenic 60.0%
GTTGTAGTGGCGGCGGATAC+GGG - chr1_1:72442003-72442022 None:intergenic 60.0%
CGTTGTAGTGGCGGCGGATA+CGG - chr1_1:72442004-72442023 None:intergenic 60.0%
GCTCCCCTCTCAACCTCTGA+AGG - chr1_1:72442484-72442503 None:intergenic 60.0%
CAAGGATGCTGCAACGGAGC+TGG + chr1_1:72444331-72444350 Msa0041870:three_prime_UTR 60.0%
CGTCGTCGGAGTCGTGAAGG+CGG - chr1_1:72441971-72441990 None:intergenic 65.0%
!! AGGGTACGTGGTGACGGAGG+CGG - chr1_1:72442031-72442050 None:intergenic 65.0%
!! GGAAGGGTACGTGGTGACGG+AGG - chr1_1:72442034-72442053 None:intergenic 65.0%
GCCACCAGGACTCACTCAGG+AGG + chr1_1:72444027-72444046 Msa0041870:CDS 65.0%
GCCTCCTGAGTGAGTCCTGG+TGG - chr1_1:72444031-72444050 None:intergenic 65.0%
AGGGCCTCCTGAGTGAGTCC+TGG - chr1_1:72444034-72444053 None:intergenic 65.0%
CGTCGGAGTCGTGAAGGCGG+TGG - chr1_1:72441968-72441987 None:intergenic 70.0%
GGCGGACGTTGTAGTGGCGG+CGG - chr1_1:72442010-72442029 None:intergenic 70.0%
GGCGGCGGACGTTGTAGTGG+CGG - chr1_1:72442013-72442032 None:intergenic 70.0%
GGAGGCGGCGGACGTTGTAG+TGG - chr1_1:72442016-72442035 None:intergenic 70.0%
!! GTACGTGGTGACGGAGGCGG+CGG - chr1_1:72442028-72442047 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 72441754 72444558 72441754 ID=Msa0041870;Name=Msa0041870
chr1_1 mRNA 72441754 72444558 72441754 ID=Msa0041870-mRNA-1;Parent=Msa0041870;Name=Msa0041870-mRNA-1;_AED=0.27;_eAED=0.27;_QI=107|1|1|1|1|1|3|279|252
chr1_1 exon 72441754 72442043 72441754 ID=Msa0041870-mRNA-1:exon:24081;Parent=Msa0041870-mRNA-1
chr1_1 exon 72442332 72442519 72442332 ID=Msa0041870-mRNA-1:exon:24082;Parent=Msa0041870-mRNA-1
chr1_1 exon 72443892 72444558 72443892 ID=Msa0041870-mRNA-1:exon:24083;Parent=Msa0041870-mRNA-1
chr1_1 five_prime_UTR 72441754 72441860 72441754 ID=Msa0041870-mRNA-1:five_prime_utr;Parent=Msa0041870-mRNA-1
chr1_1 CDS 72441861 72442043 72441861 ID=Msa0041870-mRNA-1:cds;Parent=Msa0041870-mRNA-1
chr1_1 CDS 72442332 72442519 72442332 ID=Msa0041870-mRNA-1:cds;Parent=Msa0041870-mRNA-1
chr1_1 CDS 72443892 72444279 72443892 ID=Msa0041870-mRNA-1:cds;Parent=Msa0041870-mRNA-1
chr1_1 three_prime_UTR 72444280 72444558 72444280 ID=Msa0041870-mRNA-1:three_prime_utr;Parent=Msa0041870-mRNA-1
Gene Sequence

>Msa0041870

ATGACGAGCGCTTCAGAACTATTCCACATCAGGAGACACCGATTAGGACGAAATGATATCGATTCATCTTTCCACTCTTCACCTTCTCCAGATTTCCATCATCACCACCGCCTTCACGACTCCGACGACGATGATTCTCCCCGTATCCGCCGCCACTACAACGTCCGCCGCCTCCGTCACCACGAGCGTGTATCGGAAAGATTTGATGGAAGGTATCGTCGATCTCTAGTAAATGAAGGCGGCGTTGAACCTGGAGAAAGTGTGAGAGGAACTCCAAGAGCAAGTGGAAGTGAAAGATTACCAGTAGATGTGAGACTTGCGCGAGAAAGACTCCTTCAGAGGTTGAGAGGGGAGCCTGTTGATAGAAACAGGCAAAGTGATAGAGATTCAGTGGACGAGGATCAAGAAAGTGAGTTATCAAGTGAAGTTCCAACTGAGGACTCTTTAATTACTGACTTGACTTCTCAAATGGCAAGGTTCCAGCTCTTGCATGAACAAACCAGAAAGCCACCAGGACTCACTCAGGAGGCCCTGAACTGTTTGCATCTTGAAGTTTTTAGCAGCATTGATACGGAATCAGAATCCAAGGTACTTCAGGATTGTGGGATATGCTTGGAATCTTTCGTGGATGGAGATAAACTGATCCATTTGCAATGTGGACATAAATTTCACTCTGCTTGTTTGGATCCTTGGATTCGCAGTTGTGGAGATTGTCCTTACTGTCGAAGATGTATAGTTGTAAATAGTCACCTTCAATAG
Protein sequence

>Msa0041870

MTSASELFHIRRHRLGRNDIDSSFHSSPSPDFHHHHRLHDSDDDDSPRIRRHYNVRRLRHHERVSERFDGRYRRSLVNEGGVEPGESVRGTPRASGSERLPVDVRLARERLLQRLRGEPVDRNRQSDRDSVDEDQESELSSEVPTEDSLITDLTSQMARFQLLHEQTRKPPGLTQEALNCLHLEVFSSIDTESESKVLQDCGICLESFVDGDKLIHLQCGHKFHSACLDPWIRSCGDCPYCRRCIVVNSHLQ*