AlfalfaGEDB | Msa0047540

M. sativa cultivar zhongmu-4 / Msa0047540

KEGG: K15014
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805
PFAM: PF01733.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047540	sp\|Q8VXY7\|ENT1_ARATH	65.865	416	139	2	2	415	36	450	0.0	537

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0044080	Msa0047540	0.806768	7.006621e-50	-8.615850e-47
Msa0047540	Msa0160240	0.802682	4.996077e-49	-8.615850e-47
Msa0047540	Msa1084360	0.976370	1.070874e-141	-8.615850e-47
Msa0047540	Msa1129860	0.991157	3.478257e-186	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047540	MtrunA17_Chr4g0020331	86.616	396	51	1	20	415	22	415	0.0	695
Msa0047540	MtrunA17_Chr3g0103741	85.783	415	53	3	4	415	12	423	0.0	687
Msa0047540	MtrunA17_Chr4g0063911	47.208	394	196	3	24	415	15	398	7.33e-115	341
Msa0047540	MtrunA17_Chr4g0022871	32.512	406	247	8	28	415	14	410	8.46e-58	194
Msa0047540	MtrunA17_Chr2g0280971	33.010	412	245	10	24	415	39	439	3.07e-57	194
Msa0047540	MtrunA17_Chr4g0022861	33.166	398	237	10	37	415	7	394	2.45e-55	187
Msa0047540	MtrunA17_Chr2g0280991	32.203	413	237	11	30	415	23	419	2.26e-52	181
Msa0047540	MtrunA17_Chr2g0323381	33.252	409	240	9	28	415	14	410	3.03e-51	177
Msa0047540	MtrunA17_Chr4g0022801	32.558	344	172	11	121	415	69	401	8.17e-37	138
Msa0047540	MtrunA17_Chr8g0335821	29.714	350	213	9	92	415	20	362	1.87e-29	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047540	AT1G70330.1	65.865	416	139	2	2	415	36	450	0.0	537
Msa0047540	AT1G02630.1	47.704	392	189	5	24	415	14	389	7.74e-122	359
Msa0047540	AT1G02630.2	40.816	392	172	6	24	415	14	345	1.67e-91	280
Msa0047540	AT4G05120.1	32.203	413	238	11	28	415	17	412	1.21e-50	176
Msa0047540	AT4G05120.2	32.203	413	238	11	28	415	17	412	1.21e-50	176
Msa0047540	AT4G05110.3	31.068	412	240	12	30	415	19	412	2.78e-48	170
Msa0047540	AT4G05110.1	31.068	412	240	12	30	415	19	412	2.78e-48	170
Msa0047540	AT4G05110.2	31.068	412	240	12	30	415	19	412	8.27e-48	169
Msa0047540	AT4G05130.1	30.175	401	244	11	37	415	26	412	2.53e-47	167
Msa0047540	AT1G61630.1	29.684	411	253	9	28	415	15	412	5.52e-46	164

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Find 114 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCATCAATACACTGGTA+AGG	0.536010	1_1:-80494664	None:intergenic
AACATACACGACATCAAGCA+AGG	0.573774	1_1:-80493650	None:intergenic
AAGCAAGGGAACTATGAGGA+GGG	0.636359	1_1:-80493635	None:intergenic
AAGCAAGTAATAAGGAGAAT+TGG	0.349646	1_1:-80494446	None:intergenic
AAGGTAAGAGAAGTAATCAA+CGG	0.645014	1_1:-80493464	None:intergenic
AATCTTTAAGAAGCTGAGAG+TGG	0.626615	1_1:-80494418	None:intergenic
AATGAGATTTGTGTCGGTTG+AGG	0.521136	1_1:-80493570	None:intergenic
ACAAATCTCATTCCCACGTG+AGG	0.669174	1_1:+80493581	Msa0047540:CDS
ACAATCAAGTGGTATGGGCT+CGG	0.529311	1_1:+80494335	Msa0047540:CDS
ACACATTAAAGCAAGTAATA+AGG	0.345288	1_1:-80494454	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGTAAGAGAAGTAATCAA+CGG	-	chr1_1:80493467-80493486	None:intergenic	30.0%
TGTGATTATGTTCATATGCA+TGG	+	chr1_1:80494175-80494194	Msa0047540:CDS	30.0%
TAATGAACTTAAGATTCAGG+CGG	+	chr1_1:80494241-80494260	Msa0047540:CDS	30.0%
AAGCAAGTAATAAGGAGAAT+TGG	-	chr1_1:80494449-80494468	None:intergenic	30.0%
TTTAATGTGTTTGACCTTGT+TGG	+	chr1_1:80494467-80494486	Msa0047540:CDS	30.0%
GAGAATGCAAAAATTGCAAT+CGG	+	chr1_1:80494518-80494537	Msa0047540:CDS	30.0%
AAATCATCAATACACTGGTA+AGG	-	chr1_1:80494667-80494686	None:intergenic	30.0%
AGCTAAAATCATCAATACAC+TGG	-	chr1_1:80494672-80494691	None:intergenic	30.0%
AGTGTGTTCTTCTTAGTTAT+TGG	+	chr1_1:80494737-80494756	Msa0047540:CDS	30.0%
AAGGTAAGAGAAGTAATCAA+CGG	-	chr1_1:80493467-80493486	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	80493313	80495010	80493313	ID=Msa0047540;Name=Msa0047540
chr1_1	mRNA	80493313	80495010	80493313	ID=Msa0047540-mRNA-1;Parent=Msa0047540;Name=Msa0047540-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|1\|0.5\|1\|1\|1\|2\|208\|415
chr1_1	exon	80493313	80493832	80493313	ID=Msa0047540-mRNA-1:exon:27800;Parent=Msa0047540-mRNA-1
chr1_1	exon	80494075	80495010	80494075	ID=Msa0047540-mRNA-1:exon:27801;Parent=Msa0047540-mRNA-1
chr1_1	CDS	80493313	80493832	80493313	ID=Msa0047540-mRNA-1:cds;Parent=Msa0047540-mRNA-1
chr1_1	CDS	80494075	80494802	80494075	ID=Msa0047540-mRNA-1:cds;Parent=Msa0047540-mRNA-1
chr1_1	three_prime_UTR	80494803	80495010	80494803	ID=Msa0047540-mRNA-1:three_prime_utr;Parent=Msa0047540-mRNA-1

Gene Sequence

>Msa0047540

ATGTCACAAACAGAATCCACCATTGTTCTTCTTCTACCTTCATGTTCAAGGACGACAAAGAAAGTAGCAGAAGACAAATGGCACTTAGCATATATAATCTACTTCACACTTGGCATCGGCTATTTGTTCCCATGGAACGCATTCATCACAGCCGTTGATTACTTCTCTTACCTTTACCCCGATGCAAGCGTTGACCGTATCTTTGCTCTACTTTGCACGCTTGTTAGTCTTGTTACTCTCTTCCTCATCATCATCATCCTCAACCGACACAAATCTCATTCCCACGTGAGGATCAACGTTGGATTAGCACTGTTTGTTGTTTCCCTCCTCATAGTTCCCTTGCTTGATGTCGTGTATGTTAAGGGTCGAGTCGGGTTGTATAACGGGTTTTACGTCACTGCTGGGGCTGTTGGGATTTCAGGTGTGGCTAATGCTTTGGTGCAGGGATCTATTCTTGGCTCCGCTGGTGAGTTGCCGGAGAGGTACACGCAGGCGGTCATTGCTGGGGGTGCTGCTTCTGGGGTGCTTGTTTCTTTCCTAAGGATATTTACAAAAGCTGTTTACACGCAAGATGCCTCTGGCCTGCAAAAGAGTGCTAATCTCTACTTTGCTGTATCAATTGTGATTATGTTCATATGCATGGTTTTATACAATCTAGCTCACAAACTTCCCATTATAAAGTACTATAATGAACTTAAGATTCAGGCGGCCGCCGTTGAAGACGACAATGGTCCTTTGACAGTATCTGTCTGGAGATCAACTGTATGGGATACCTTAGGAACAATCAAGTGGTATGGGCTCGGCATCATGCTCATTTATGTTGTGACGCTTGCAATATTTCCTGGTTACATTACAGAAGATGTCCACTCTCAGCTTCTTAAAGATTGGTATCCAATTCTCCTTATTACTTGCTTTAATGTGTTTGACCTTGTTGGCAAAAGCCTCACTGCAGTATACCTTCTAGAGAATGCAAAAATTGCAATCGGTTGTTGCATAGCAAGACTGTTGTTTTTCCCTCTCTTCTTAGGATGCTTGCACGGTCCGAAATTCTTCCGGACAGAGATTCCTGTGACACTATTAACGTGTCTCTTAGGGCTTACAAATGGATACCTTACCAGTGTATTGATGATTTTAGCTCCTAAAACCGTGAAGTTGCAGCATGCAGAGACTGCGGGGATTGTGAGTGTGTTCTTCTTAGTTATTGGTCTGGCTGCTGGCTCTATTATAGCTTGGTTTTGGGTCATCTGA

Protein sequence

>Msa0047540

MSQTESTIVLLLPSCSRTTKKVAEDKWHLAYIIYFTLGIGYLFPWNAFITAVDYFSYLYPDASVDRIFALLCTLVSLVTLFLIIIILNRHKSHSHVRINVGLALFVVSLLIVPLLDVVYVKGRVGLYNGFYVTAGAVGISGVANALVQGSILGSAGELPERYTQAVIAGGAASGVLVSFLRIFTKAVYTQDASGLQKSANLYFAVSIVIMFICMVLYNLAHKLPIIKYYNELKIQAAAVEDDNGPLTVSVWRSTVWDTLGTIKWYGLGIMLIYVVTLAIFPGYITEDVHSQLLKDWYPILLITCFNVFDLVGKSLTAVYLLENAKIAIGCCIARLLFFPLFLGCLHGPKFFRTEIPVTLLTCLLGLTNGYLTSVLMILAPKTVKLQHAETAGIVSVFFLVIGLAAGSIIAWFWVI*