AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0044080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044080 MtrunA17_Chr1g0210681 98.765 243 3 0 1 243 1 243 2.36e-178 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 88 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTCTTTGTTTGTTATC+TGG 0.223276 1_1:+75550928 None:intergenic
ATTTCCCCGATCTCGATTTC+TGG 0.228924 1_1:-75550908 Msa0044080:five_prime_UTR
GGTATGCGAAGATTAGTAAT+AGG 0.261485 1_1:+75550767 None:intergenic
ATCGATCTTCCCTTACTTCT+CGG 0.290746 1_1:-75550655 Msa0044080:CDS
ATATTGAATTGGCTGTTAAA+TGG 0.303433 1_1:-75550339 Msa0044080:CDS
CATGATTGGCGAACGAGTTT+AGG 0.319356 1_1:+75550831 None:intergenic
CTTATAGATGTGTTCCTACT+TGG 0.325100 1_1:-75550387 Msa0044080:CDS
GCAATCTGTAACCAGATAAT+AGG 0.326793 1_1:+75548293 None:intergenic
TAGTCAATGAATCACTTCTT+TGG 0.328386 1_1:+75548447 None:intergenic
TTACTGCCATATAACTTTGC+AGG 0.331845 1_1:+75548018 None:intergenic
TTAAATGGGCGCTTCGTAAG+AGG 0.341862 1_1:-75550324 Msa0044080:CDS
TTAATCTAGAACCTATTATC+TGG 0.346208 1_1:-75548304 Msa0044080:three_prime_UTR
ATGAATCACTTCTTTGGCTT+CGG 0.351012 1_1:+75548453 None:intergenic
TGTCTGGAAAACCATCAAAC+TGG 0.367415 1_1:+75548402 None:intergenic
GAGATGCCTGCAAAGTTATA+TGG 0.378395 1_1:-75548024 Msa0044080:three_prime_UTR
CTAAACTCGTTCGCCAATCA+TGG 0.389303 1_1:-75550830 Msa0044080:exon
CCATCATGAACCTTACTATA+AGG 0.393189 1_1:+75550523 None:intergenic
TGGTGGTTGGCCTTCTTCGC+CGG 0.404259 1_1:-75550491 Msa0044080:CDS
GTATGCGAAGATTAGTAATA+GGG 0.406202 1_1:+75550768 None:intergenic
TCAACCTGGTGATATTGAAT+TGG 0.421228 1_1:-75550350 Msa0044080:CDS
AAGATAATGAGTGAAACAAT+TGG 0.423033 1_1:+75550610 None:intergenic
CAAGTAGGAACACATCTATA+AGG 0.427274 1_1:+75550388 None:intergenic
AGAAATCGAGATCGGGGAAA+TGG 0.441800 1_1:+75550910 None:intergenic
CACTAGGTGTCACAGATGAT+TGG 0.449809 1_1:-75548578 Msa0044080:CDS
ATGCTCATATTATGAAAAGT+TGG 0.462906 1_1:-75548203 Msa0044080:three_prime_UTR
AAGAACCAGAAATCGAGATC+GGG 0.463765 1_1:+75550903 None:intergenic
TATTGAATTGGCTGTTAAAT+GGG 0.466528 1_1:-75550338 Msa0044080:CDS
TCTGGTTCTTCTTCTTCCTA+CGG 0.468824 1_1:-75550890 Msa0044080:five_prime_UTR
TCAATAATAGCAGGATCACC+CGG 0.471488 1_1:+75550457 None:intergenic
GATATGCCTACACCCATGAT+TGG 0.481126 1_1:+75550817 None:intergenic
ATGAGACTAAGGATGAATAG+AGG 0.490069 1_1:+75550707 None:intergenic
CGGTGAACCACCTTATAGTA+AGG 0.492096 1_1:-75550533 Msa0044080:CDS
TAACTTGCAATCATTATCCA+TGG 0.494656 1_1:+75548147 None:intergenic
TTATAGATGTGTTCCTACTT+GGG 0.497653 1_1:-75550386 Msa0044080:CDS
GAGAGCATTCATGCGAATGT+AGG 0.504524 1_1:+75548521 None:intergenic
AGATAATGAGTGAAACAATT+GGG 0.508906 1_1:+75550611 None:intergenic
ATCGCAAGGCACTTGAAGAA+TGG 0.514773 1_1:-75548497 Msa0044080:CDS
TGGATAGAATTAAGAAGCCT+AGG 0.521479 1_1:+75550737 None:intergenic
GAAGAACCAGAAATCGAGAT+CGG 0.525993 1_1:+75550902 None:intergenic
TGAGACGAGGAATGAGACTA+AGG 0.528904 1_1:+75550696 None:intergenic
AGTAAGGGAAGATCGATGGA+AGG 0.531826 1_1:+75550661 None:intergenic
TTCGCCGGAAAAGATGCCCC+CGG 0.532390 1_1:-75550476 Msa0044080:CDS
CAAGTACAATCAATAATAGC+AGG 0.535238 1_1:+75550448 None:intergenic
GATTGGCGAACGAGTTTAGG+AGG 0.537384 1_1:+75550834 None:intergenic
ATTCTTCAAGTGCCTTGCGA+TGG 0.543540 1_1:+75548499 None:intergenic
ATTCGAACGAAGTAAAGATA+AGG 0.546264 1_1:+75550583 None:intergenic
AGAGCATTCATGCGAATGTA+GGG 0.548827 1_1:+75548522 None:intergenic
CATGATGGACTTTACGTTGG+TGG 0.548990 1_1:-75550508 Msa0044080:CDS
GGATGAATAGAGGAATGGAA+TGG 0.558145 1_1:+75550717 None:intergenic
GTAAGGGAAGATCGATGGAA+GGG 0.569098 1_1:+75550662 None:intergenic
TGTTATCTGGTTGACGCACG+CGG 0.570109 1_1:+75550941 None:intergenic
TAAACTCGTTCGCCAATCAT+GGG 0.571503 1_1:-75550829 Msa0044080:exon
TAAATGGGCGCTTCGTAAGA+GGG 0.572086 1_1:-75550323 Msa0044080:CDS
CCTTATAGTAAGGTTCATGA+TGG 0.572606 1_1:-75550523 Msa0044080:CDS
GTCTGGAAAACCATCAAACT+GGG 0.576734 1_1:+75548403 None:intergenic
CGTTCGCCAATCATGGGTGT+AGG 0.577179 1_1:-75550823 Msa0044080:exon
AATTGGGAATGTTCCGTCGG+AGG 0.578873 1_1:+75550627 None:intergenic
CAATAATAGCAGGATCACCC+GGG 0.581474 1_1:+75550458 None:intergenic
CCGAGGTTGAAAAGTTTGAG+TGG 0.583549 1_1:-75550418 Msa0044080:CDS
ATGGACTTTACGTTGGTGGT+TGG 0.584648 1_1:-75550504 Msa0044080:CDS
ACTAAGGATGAATAGAGGAA+TGG 0.589227 1_1:+75550712 None:intergenic
AATTCAATATCACCAGGTTG+AGG 0.590249 1_1:+75550352 None:intergenic
ATTGCATCGTGTAATGCCCA+TGG 0.591116 1_1:-75548164 Msa0044080:three_prime_UTR
GAGAAGTAAGGGAAGATCGA+TGG 0.593587 1_1:+75550657 None:intergenic
ATTGTACTTGTGAGTTTCCG+AGG 0.596378 1_1:-75550435 Msa0044080:CDS
ACAGCCAATTCAATATCACC+AGG 0.601105 1_1:+75550346 None:intergenic
CATGAATGCTCTCCATCGCA+AGG 0.603132 1_1:-75548511 Msa0044080:CDS
AACTTGCAATCATTATCCAT+GGG 0.603454 1_1:+75548148 None:intergenic
CAAGAATGCTAATAAAGTAG+AGG 0.605988 1_1:+75548335 None:intergenic
AGGTTGAAAAGTTTGAGTGG+TGG 0.609401 1_1:-75550415 Msa0044080:CDS
AACAATTGGGAATGTTCCGT+CGG 0.616528 1_1:+75550624 None:intergenic
ATGGGCATTACACGATGCAA+TGG 0.619638 1_1:+75548166 None:intergenic
TGAGATTGTTTATGACACAA+TGG 0.622404 1_1:-75548048 Msa0044080:three_prime_UTR
GTTCATGATGGACTTTACGT+TGG 0.636648 1_1:-75550511 Msa0044080:CDS
GGAGATCTAGTATCCCAAGT+AGG 0.645762 1_1:+75550373 None:intergenic
AGAACCAGAAATCGAGATCG+GGG 0.646478 1_1:+75550904 None:intergenic
GATGTGTTGAAGTTGAACAA+TGG 0.646551 1_1:-75548227 Msa0044080:three_prime_UTR
TCATGAACCTTACTATAAGG+TGG 0.649373 1_1:+75550526 None:intergenic
GCTAATAAAGTAGAGGCACA+AGG 0.650620 1_1:+75548342 None:intergenic
CGATGCAATGGTCTCAACTG+TGG 0.653190 1_1:+75548178 None:intergenic
GATACTAGATCTCCTCAACC+TGG 0.658121 1_1:-75550364 Msa0044080:CDS
GGAGTTGTATGTAATTGTTG+TGG 0.658660 1_1:-75547927 Msa0044080:three_prime_UTR
CTTCTCGGCAAAACCTCCGA+CGG 0.659592 1_1:-75550640 Msa0044080:CDS
TGTGCACTGTTAGTTGCACT+AGG 0.661928 1_1:-75548594 Msa0044080:CDS
GAAACTGCAACATCAACCGT+AGG 0.667737 1_1:+75550874 None:intergenic
GGTGTGATGCGATTGAGACG+AGG 0.672751 1_1:+75550683 None:intergenic
AATAATAGCAGGATCACCCG+GGG 0.690073 1_1:+75550459 None:intergenic
ATAATAGCAGGATCACCCGG+GGG 0.807868 1_1:+75550460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTGATTATTTTTACTC+CGG + chr1_1:75548902-75548921 None:intergenic 15.0%
!!! TATTATAATTTTGCTAGTTT+TGG - chr1_1:75549511-75549530 Msa0044080:intron 15.0%
!! ATAAATGAAAATGAGTAAAA+AGG + chr1_1:75549823-75549842 None:intergenic 15.0%
!! ATCTTAGTTGAAAAATAAAT+TGG - chr1_1:75550879-75550898 Msa0044080:five_prime_UTR 15.0%
!! TACTACAATATTTCATTTCA+TGG + chr1_1:75548603-75548622 None:intergenic 20.0%
!!! ATTTCATTTTATGAGAGTAA+TGG - chr1_1:75549226-75549245 Msa0044080:intron 20.0%
!!! TTTTCTATGTTGTTTGATAA+AGG - chr1_1:75549317-75549336 Msa0044080:intron 20.0%
!!! TTTCTATGTTGTTTGATAAA+GGG - chr1_1:75549318-75549337 Msa0044080:intron 20.0%
!! AAAACAAAACAAAATGATGT+TGG + chr1_1:75550170-75550189 None:intergenic 20.0%
!!! TATATGGCAGTAAATTTTAT+TGG - chr1_1:75550819-75550838 Msa0044080:exon 20.0%
!!! TATTTTTCAACTAAGATCTT+TGG + chr1_1:75550876-75550895 None:intergenic 20.0%
! AGATAATGAGTGAAACAATT+GGG + chr1_1:75548219-75548238 None:intergenic 25.0%
! AAGATAATGAGTGAAACAAT+TGG + chr1_1:75548220-75548239 None:intergenic 25.0%
! ATATTGAATTGGCTGTTAAA+TGG - chr1_1:75548488-75548507 Msa0044080:CDS 25.0%
! TATTGAATTGGCTGTTAAAT+GGG - chr1_1:75548489-75548508 Msa0044080:CDS 25.0%
! TAAGCACAATGAACAAAAAT+GGG + chr1_1:75548541-75548560 None:intergenic 25.0%
! ATAAGCACAATGAACAAAAA+TGG + chr1_1:75548542-75548561 None:intergenic 25.0%
!! ATTTTTGTTCATTGTGCTTA+TGG - chr1_1:75548541-75548560 Msa0044080:CDS 25.0%
! TTGTTAGTATGAATGTAACT+AGG - chr1_1:75548723-75548742 Msa0044080:intron 25.0%
!! GTTCAAAACAACTTTAGAAA+AGG + chr1_1:75548996-75549015 None:intergenic 25.0%
!!! CTTTGGATTAGTGTATATAT+TGG + chr1_1:75549093-75549112 None:intergenic 25.0%
! AACAACATAGAAAACTGTAA+GGG + chr1_1:75549311-75549330 None:intergenic 25.0%
! AAACAACATAGAAAACTGTA+AGG + chr1_1:75549312-75549331 None:intergenic 25.0%
! AGTAAAATTCTTTCTTCCAA+TGG + chr1_1:75549492-75549511 None:intergenic 25.0%
! TGTATGATATGAATAGACTA+GGG + chr1_1:75549847-75549866 None:intergenic 25.0%
! TTGTATGTTTAATTCATCTC+TGG + chr1_1:75549938-75549957 None:intergenic 25.0%
! AGTGATACTATTATATTCCT+AGG - chr1_1:75549961-75549980 Msa0044080:intron 25.0%
! TAATATAGTGAAACTCTCAT+TGG - chr1_1:75550041-75550060 Msa0044080:intron 25.0%
!! TGTAACTTGTAAGTTAAATG+TGG + chr1_1:75550077-75550096 None:intergenic 25.0%
! TGGTTATATCACATTCTTAT+AGG + chr1_1:75550150-75550169 None:intergenic 25.0%
! AAAAGCTGATAAAGCAAAAA+CGG - chr1_1:75550282-75550301 Msa0044080:intron 25.0%
!! TTAATCTAGAACCTATTATC+TGG - chr1_1:75550523-75550542 Msa0044080:CDS 25.0%
! ATGCTCATATTATGAAAAGT+TGG - chr1_1:75550624-75550643 Msa0044080:CDS 25.0%
!!! ATTCTGAAGTTTTGATGTAA+TGG - chr1_1:75550925-75550944 Msa0044080:five_prime_UTR 25.0%
!! AATGGTCTTTGTTTGTTATC+TGG + chr1_1:75547902-75547921 None:intergenic 30.0%
GTATGCGAAGATTAGTAATA+GGG + chr1_1:75548062-75548081 None:intergenic 30.0%
!!! TTAAGAAGCCTAGGTTTTTT+AGG + chr1_1:75548084-75548103 None:intergenic 30.0%
ATTCGAACGAAGTAAAGATA+AGG + chr1_1:75548247-75548266 None:intergenic 30.0%
CAAGTACAATCAATAATAGC+AGG + chr1_1:75548382-75548401 None:intergenic 30.0%
TTATAGATGTGTTCCTACTT+GGG - chr1_1:75548441-75548460 Msa0044080:exon 30.0%
TGTTAACCAAAAGAAACCTA+GGG + chr1_1:75548869-75548888 None:intergenic 30.0%
ATGTTAACCAAAAGAAACCT+AGG + chr1_1:75548870-75548889 None:intergenic 30.0%
!! TATTTTTACTCCGGTGTATA+TGG + chr1_1:75548893-75548912 None:intergenic 30.0%
! TGTAGGTTGAAAATGTTACA+AGG + chr1_1:75548927-75548946 None:intergenic 30.0%
CCATGCTATCATATAATTGT+AGG + chr1_1:75548944-75548963 None:intergenic 30.0%
CCTACAATTATATGATAGCA+TGG - chr1_1:75548941-75548960 Msa0044080:intron 30.0%
!! CTTTAGAAAAGGCATTTGTT+TGG + chr1_1:75548985-75549004 None:intergenic 30.0%
TTCTAAATTTGTGCTTGTCA+AGG - chr1_1:75549017-75549036 Msa0044080:intron 30.0%
CCAATTTAGATAGGAGAATT+TGG + chr1_1:75549117-75549136 None:intergenic 30.0%
CCAAATTCTCCTATCTAAAT+TGG - chr1_1:75549114-75549133 Msa0044080:intron 30.0%
GTCTCAATTCCAATTTAGAT+AGG + chr1_1:75549126-75549145 None:intergenic 30.0%
CTTTCACATAAATCAGAATG+TGG + chr1_1:75549189-75549208 None:intergenic 30.0%
ACAACATAGAAAACTGTAAG+GGG + chr1_1:75549310-75549329 None:intergenic 30.0%
TAAACACTTAAGACATGTGA+GGG + chr1_1:75549417-75549436 None:intergenic 30.0%
!! ATTATCTGTTGTTTCCTTGA+AGG + chr1_1:75549461-75549480 None:intergenic 30.0%
CAGATAATTGAGATAACCAT+TGG - chr1_1:75549473-75549492 Msa0044080:intron 30.0%
! ATCAACAACACTGTTTGTTT+TGG - chr1_1:75549552-75549571 Msa0044080:intron 30.0%
ACTTTCGTGTTCATTATCTT+CGG - chr1_1:75549585-75549604 Msa0044080:intron 30.0%
! ATTGCATGTGTGTTATTCTA+AGG + chr1_1:75549722-75549741 None:intergenic 30.0%
ACACATGCAATGAAAAAACT+GGG - chr1_1:75549731-75549750 Msa0044080:intron 30.0%
AAAAGCCCATATTGTCAATT+AGG - chr1_1:75549769-75549788 Msa0044080:intron 30.0%
TCCTACCTAATTGACAATAT+GGG + chr1_1:75549777-75549796 None:intergenic 30.0%
ATCCTACCTAATTGACAATA+TGG + chr1_1:75549778-75549797 None:intergenic 30.0%
GTGTATGATATGAATAGACT+AGG + chr1_1:75549848-75549867 None:intergenic 30.0%
TTTGTGCTTATAATAGTGAC+CGG + chr1_1:75549874-75549893 None:intergenic 30.0%
TAAGTTAAATGTGGAGACAT+TGG + chr1_1:75550068-75550087 None:intergenic 30.0%
TAGTCAATGAATCACTTCTT+TGG + chr1_1:75550383-75550402 None:intergenic 30.0%
! GTTTTCCAGACAATACTTAA+TGG - chr1_1:75550436-75550455 Msa0044080:CDS 30.0%
!! TTTTGCCATTAAGTATTGTC+TGG + chr1_1:75550444-75550463 None:intergenic 30.0%
CAAGAATGCTAATAAAGTAG+AGG + chr1_1:75550495-75550514 None:intergenic 30.0%
AACTTGCAATCATTATCCAT+GGG + chr1_1:75550682-75550701 None:intergenic 30.0%
! TAACTTGCAATCATTATCCA+TGG + chr1_1:75550683-75550702 None:intergenic 30.0%
! CTCAAGTTTTTCTTTGCATA+AGG - chr1_1:75550738-75550757 Msa0044080:CDS 30.0%
TGAGATTGTTTATGACACAA+TGG - chr1_1:75550779-75550798 Msa0044080:CDS 30.0%
GGTATGCGAAGATTAGTAAT+AGG + chr1_1:75548063-75548082 None:intergenic 35.0%
TTCGCATACCTAAAAAACCT+AGG - chr1_1:75548073-75548092 Msa0044080:three_prime_UTR 35.0%
TGGATAGAATTAAGAAGCCT+AGG + chr1_1:75548093-75548112 None:intergenic 35.0%
ACTAAGGATGAATAGAGGAA+TGG + chr1_1:75548118-75548137 None:intergenic 35.0%
ATGAGACTAAGGATGAATAG+AGG + chr1_1:75548123-75548142 None:intergenic 35.0%
TCATGAACCTTACTATAAGG+TGG + chr1_1:75548304-75548323 None:intergenic 35.0%
CCATCATGAACCTTACTATA+AGG + chr1_1:75548307-75548326 None:intergenic 35.0%
CCTTATAGTAAGGTTCATGA+TGG - chr1_1:75548304-75548323 Msa0044080:three_prime_UTR 35.0%
CAAGTAGGAACACATCTATA+AGG + chr1_1:75548442-75548461 None:intergenic 35.0%
CTTATAGATGTGTTCCTACT+TGG - chr1_1:75548440-75548459 Msa0044080:exon 35.0%
AATTCAATATCACCAGGTTG+AGG + chr1_1:75548478-75548497 None:intergenic 35.0%
TCAACCTGGTGATATTGAAT+TGG - chr1_1:75548477-75548496 Msa0044080:CDS 35.0%
! CTGTTGTTTGTAAACTCTGA+AGG + chr1_1:75548750-75548769 None:intergenic 35.0%
! CCACTAGTTTATTACCATCA+AGG + chr1_1:75548807-75548826 None:intergenic 35.0%
! CCTTGATGGTAATAAACTAG+TGG - chr1_1:75548804-75548823 Msa0044080:intron 35.0%
!!! AGTGGTTATGTTAGTTTTCC+AGG - chr1_1:75548822-75548841 Msa0044080:intron 35.0%
TAAGTTCAAGGTTCAAATCC+TGG + chr1_1:75548843-75548862 None:intergenic 35.0%
! TTAACTCCCTAGGTTTCTTT+TGG - chr1_1:75548860-75548879 Msa0044080:intron 35.0%
TGGTTAACATCCATATACAC+CGG - chr1_1:75548880-75548899 Msa0044080:intron 35.0%
TGCTTGTCAAGGAAAAACTT+TGG - chr1_1:75549028-75549047 Msa0044080:intron 35.0%
AGATAGGAGAATTTGGACTT+TGG + chr1_1:75549110-75549129 None:intergenic 35.0%
TCTAAATTGGAATTGAGACG+AGG - chr1_1:75549127-75549146 Msa0044080:intron 35.0%
AGAATGTGGTCACAAACTAA+TGG + chr1_1:75549175-75549194 None:intergenic 35.0%
! ATGTTGTTTGATAAAGGGAG+CGG - chr1_1:75549323-75549342 Msa0044080:intron 35.0%
! GGTTCTTTTCTCTCTTTAGT+TGG + chr1_1:75549396-75549415 None:intergenic 35.0%
CTAAACACTTAAGACATGTG+AGG + chr1_1:75549418-75549437 None:intergenic 35.0%
TTGATGCCAATAGTGTACAA+CGG + chr1_1:75549537-75549556 None:intergenic 35.0%
CACACATGCAATGAAAAAAC+TGG - chr1_1:75549730-75549749 Msa0044080:intron 35.0%
TATTCATATCATACACCCTC+CGG - chr1_1:75549852-75549871 Msa0044080:intron 35.0%
TATAGGCAAGCATAATAGTC+TGG + chr1_1:75550133-75550152 None:intergenic 35.0%
! GTTTTCCCTTTCTTGTGTAA+AGG - chr1_1:75550185-75550204 Msa0044080:intron 35.0%
CATGACCTTTACACAAGAAA+GGG + chr1_1:75550193-75550212 None:intergenic 35.0%
! ATGAATCACTTCTTTGGCTT+CGG + chr1_1:75550377-75550396 None:intergenic 35.0%
! TTTTGTCAATCCCAGTTTGA+TGG - chr1_1:75550414-75550433 Msa0044080:CDS 35.0%
GCAATCTGTAACCAGATAAT+AGG + chr1_1:75550537-75550556 None:intergenic 35.0%
GATGTGTTGAAGTTGAACAA+TGG - chr1_1:75550600-75550619 Msa0044080:CDS 35.0%
TGCAAAGAAAAACTTGAGCT+TGG + chr1_1:75550736-75550755 None:intergenic 35.0%
TTACTGCCATATAACTTTGC+AGG + chr1_1:75550812-75550831 None:intergenic 35.0%
GGAGTTGTATGTAATTGTTG+TGG - chr1_1:75550900-75550919 Msa0044080:five_prime_UTR 35.0%
!! GTTTTGATGTAATGGTGTGA+TGG - chr1_1:75550933-75550952 Msa0044080:five_prime_UTR 35.0%
AAGAACCAGAAATCGAGATC+GGG + chr1_1:75547927-75547946 None:intergenic 40.0%
GAAGAACCAGAAATCGAGAT+CGG + chr1_1:75547928-75547947 None:intergenic 40.0%
!! TCTGGTTCTTCTTCTTCCTA+CGG - chr1_1:75547937-75547956 Msa0044080:three_prime_UTR 40.0%
TAAACTCGTTCGCCAATCAT+GGG - chr1_1:75547998-75548017 Msa0044080:three_prime_UTR 40.0%
GGATGAATAGAGGAATGGAA+TGG + chr1_1:75548113-75548132 None:intergenic 40.0%
ATCGATCTTCCCTTACTTCT+CGG - chr1_1:75548172-75548191 Msa0044080:three_prime_UTR 40.0%
AACAATTGGGAATGTTCCGT+CGG + chr1_1:75548206-75548225 None:intergenic 40.0%
!! GTTCATGATGGACTTTACGT+TGG - chr1_1:75548316-75548335 Msa0044080:three_prime_UTR 40.0%
TCAATAATAGCAGGATCACC+CGG + chr1_1:75548373-75548392 None:intergenic 40.0%
ATTGTACTTGTGAGTTTCCG+AGG - chr1_1:75548392-75548411 Msa0044080:three_prime_UTR 40.0%
! CCACTCAAACTTTTCAACCT+CGG + chr1_1:75548412-75548431 None:intergenic 40.0%
AGGTTGAAAAGTTTGAGTGG+TGG - chr1_1:75548412-75548431 Msa0044080:three_prime_UTR 40.0%
ACAGCCAATTCAATATCACC+AGG + chr1_1:75548484-75548503 None:intergenic 40.0%
AACCTTGAACTTAACTCCCT+AGG - chr1_1:75548850-75548869 Msa0044080:intron 40.0%
AACCTAGGGAGTTAAGTTCA+AGG + chr1_1:75548855-75548874 None:intergenic 40.0%
!! GAAAAGGCATTTGTTTGGAC+AGG + chr1_1:75548980-75548999 None:intergenic 40.0%
TCTGCACGTGAGATAATAGA+AGG - chr1_1:75549284-75549303 Msa0044080:intron 40.0%
ATAAAGGGAGCGGTGATAAA+GGG - chr1_1:75549333-75549352 Msa0044080:intron 40.0%
GATAAAGGGAAATGTTGGAC+AGG - chr1_1:75549347-75549366 Msa0044080:intron 40.0%
TTTGTACTGTGAGTCCTTCA+AGG - chr1_1:75549444-75549463 Msa0044080:intron 40.0%
! TTTTGGCCGTTGTACACTAT+TGG - chr1_1:75549528-75549547 Msa0044080:intron 40.0%
TGCTAAACACCCAGTAAACA+GGG + chr1_1:75549634-75549653 None:intergenic 40.0%
GCCCATATTGTCAATTAGGT+AGG - chr1_1:75549773-75549792 Msa0044080:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr1_1:75549870-75549889 None:intergenic 40.0%
CCATGACCTTTACACAAGAA+AGG + chr1_1:75550194-75550213 None:intergenic 40.0%
CCTTTCTTGTGTAAAGGTCA+TGG - chr1_1:75550191-75550210 Msa0044080:intron 40.0%
! AGAGCATTCATGCGAATGTA+GGG + chr1_1:75550308-75550327 None:intergenic 40.0%
GTCTGGAAAACCATCAAACT+GGG + chr1_1:75550427-75550446 None:intergenic 40.0%
TGTCTGGAAAACCATCAAAC+TGG + chr1_1:75550428-75550447 None:intergenic 40.0%
! GCTAATAAAGTAGAGGCACA+AGG + chr1_1:75550488-75550507 None:intergenic 40.0%
GAGATGCCTGCAAAGTTATA+TGG - chr1_1:75550803-75550822 Msa0044080:CDS 40.0%
AGAAATCGAGATCGGGGAAA+TGG + chr1_1:75547920-75547939 None:intergenic 45.0%
ATTTCCCCGATCTCGATTTC+TGG - chr1_1:75547919-75547938 Msa0044080:three_prime_UTR 45.0%
AGAACCAGAAATCGAGATCG+GGG + chr1_1:75547926-75547945 None:intergenic 45.0%
GAAACTGCAACATCAACCGT+AGG + chr1_1:75547956-75547975 None:intergenic 45.0%
CATGATTGGCGAACGAGTTT+AGG + chr1_1:75547999-75548018 None:intergenic 45.0%
CTAAACTCGTTCGCCAATCA+TGG - chr1_1:75547997-75548016 Msa0044080:three_prime_UTR 45.0%
GATATGCCTACACCCATGAT+TGG + chr1_1:75548013-75548032 None:intergenic 45.0%
TGAGACGAGGAATGAGACTA+AGG + chr1_1:75548134-75548153 None:intergenic 45.0%
GTAAGGGAAGATCGATGGAA+GGG + chr1_1:75548168-75548187 None:intergenic 45.0%
AGTAAGGGAAGATCGATGGA+AGG + chr1_1:75548169-75548188 None:intergenic 45.0%
GAGAAGTAAGGGAAGATCGA+TGG + chr1_1:75548173-75548192 None:intergenic 45.0%
! GAGGTTTTGCCGAGAAGTAA+GGG + chr1_1:75548184-75548203 None:intergenic 45.0%
CGGTGAACCACCTTATAGTA+AGG - chr1_1:75548294-75548313 Msa0044080:three_prime_UTR 45.0%
!! CATGATGGACTTTACGTTGG+TGG - chr1_1:75548319-75548338 Msa0044080:three_prime_UTR 45.0%
!! ATGGACTTTACGTTGGTGGT+TGG - chr1_1:75548323-75548342 Msa0044080:three_prime_UTR 45.0%
AATAATAGCAGGATCACCCG+GGG + chr1_1:75548371-75548390 None:intergenic 45.0%
CAATAATAGCAGGATCACCC+GGG + chr1_1:75548372-75548391 None:intergenic 45.0%
CCGAGGTTGAAAAGTTTGAG+TGG - chr1_1:75548409-75548428 Msa0044080:three_prime_UTR 45.0%
GGAGATCTAGTATCCCAAGT+AGG + chr1_1:75548457-75548476 None:intergenic 45.0%
GATACTAGATCTCCTCAACC+TGG - chr1_1:75548463-75548482 Msa0044080:CDS 45.0%
TTAAATGGGCGCTTCGTAAG+AGG - chr1_1:75548503-75548522 Msa0044080:CDS 45.0%
TAAATGGGCGCTTCGTAAGA+GGG - chr1_1:75548504-75548523 Msa0044080:CDS 45.0%
TGACCCCACTTAGTGGAATA+AGG - chr1_1:75548689-75548708 Msa0044080:intron 45.0%
!! GAGCCTTATTCCACTAAGTG+GGG + chr1_1:75548695-75548714 None:intergenic 45.0%
!! CGAGCCTTATTCCACTAAGT+GGG + chr1_1:75548696-75548715 None:intergenic 45.0%
CCACTTAGTGGAATAAGGCT+CGG - chr1_1:75548694-75548713 Msa0044080:intron 45.0%
TGCACACCAATGACTTATGC+TGG - chr1_1:75548780-75548799 Msa0044080:intron 45.0%
CAAGGTCCAGCATAAGTCAT+TGG + chr1_1:75548789-75548808 None:intergenic 45.0%
TGACTTATGCTGGACCTTGA+TGG - chr1_1:75548790-75548809 Msa0044080:intron 45.0%
GATAAAGGGAGCGGTGATAA+AGG - chr1_1:75549332-75549351 Msa0044080:intron 45.0%
GCGGTGATAAAGGGAAATGT+TGG - chr1_1:75549342-75549361 Msa0044080:intron 45.0%
GTGCTAAACACCCAGTAAAC+AGG + chr1_1:75549635-75549654 None:intergenic 45.0%
GTGCTTATAATAGTGACCGG+AGG + chr1_1:75549871-75549890 None:intergenic 45.0%
ATATTCCTAGGTCCCTGCAT+AGG - chr1_1:75549973-75549992 Msa0044080:intron 45.0%
TGTGCACTGTTAGTTGCACT+AGG - chr1_1:75550233-75550252 Msa0044080:intron 45.0%
! CACTAGGTGTCACAGATGAT+TGG - chr1_1:75550249-75550268 Msa0044080:intron 45.0%
!! GAGAGCATTCATGCGAATGT+AGG + chr1_1:75550309-75550328 None:intergenic 45.0%
ATTCTTCAAGTGCCTTGCGA+TGG + chr1_1:75550331-75550350 None:intergenic 45.0%
! ATCGCAAGGCACTTGAAGAA+TGG - chr1_1:75550330-75550349 Msa0044080:CDS 45.0%
ATGGGCATTACACGATGCAA+TGG + chr1_1:75550664-75550683 None:intergenic 45.0%
ATTGCATCGTGTAATGCCCA+TGG - chr1_1:75550663-75550682 Msa0044080:CDS 45.0%
TGTTATCTGGTTGACGCACG+CGG + chr1_1:75547889-75547908 None:intergenic 50.0%
GATTGGCGAACGAGTTTAGG+AGG + chr1_1:75547996-75548015 None:intergenic 50.0%
! GGAGGTTTTGCCGAGAAGTA+AGG + chr1_1:75548185-75548204 None:intergenic 50.0%
AATTGGGAATGTTCCGTCGG+AGG + chr1_1:75548203-75548222 None:intergenic 50.0%
!!! GCTTTTTCGTCTTCGAAGCG+CGG + chr1_1:75548275-75548294 None:intergenic 50.0%
GCGCTTCGAAGACGAAAAAG+CGG - chr1_1:75548274-75548293 Msa0044080:three_prime_UTR 50.0%
ATAATAGCAGGATCACCCGG+GGG + chr1_1:75548370-75548389 None:intergenic 50.0%
ACTAAGTGGGGTCAGCTACA+TGG + chr1_1:75548683-75548702 None:intergenic 50.0%
ATGTAGCTGACCCCACTTAG+TGG - chr1_1:75548682-75548701 Msa0044080:intron 50.0%
!! CCGAGCCTTATTCCACTAAG+TGG + chr1_1:75548697-75548716 None:intergenic 50.0%
CTTAGTGGAATAAGGCTCGG+TGG - chr1_1:75548697-75548716 Msa0044080:intron 50.0%
AACTCCTCGCCCTGTTTACT+GGG - chr1_1:75549622-75549641 Msa0044080:intron 50.0%
TCAGTGCACATGCCTATGCA+GGG + chr1_1:75549988-75550007 None:intergenic 50.0%
ATCAGTGCACATGCCTATGC+AGG + chr1_1:75549989-75550008 None:intergenic 50.0%
CATGAATGCTCTCCATCGCA+AGG - chr1_1:75550316-75550335 Msa0044080:CDS 50.0%
CGATGCAATGGTCTCAACTG+TGG + chr1_1:75550652-75550671 None:intergenic 50.0%
CGTTCGCCAATCATGGGTGT+AGG - chr1_1:75548004-75548023 Msa0044080:three_prime_UTR 55.0%
GGTGTGATGCGATTGAGACG+AGG + chr1_1:75548147-75548166 None:intergenic 55.0%
CTTCTCGGCAAAACCTCCGA+CGG - chr1_1:75548187-75548206 Msa0044080:three_prime_UTR 55.0%
! GGCATCTTTTCCGGCGAAGA+AGG + chr1_1:75548349-75548368 None:intergenic 55.0%
GAACTCCTCGCCCTGTTTAC+TGG - chr1_1:75549621-75549640 Msa0044080:intron 55.0%
AACACCCAGTAAACAGGGCG+AGG + chr1_1:75549629-75549648 None:intergenic 55.0%
ACATGCCTATGCAGGGACCT+AGG + chr1_1:75549981-75550000 None:intergenic 55.0%
!! TGGTGGTTGGCCTTCTTCGC+CGG - chr1_1:75548336-75548355 Msa0044080:three_prime_UTR 60.0%
TTCGCCGGAAAAGATGCCCC+CGG - chr1_1:75548351-75548370 Msa0044080:three_prime_UTR 60.0%
!!! TCACCCGGGGGCATCTTTTC+CGG + chr1_1:75548358-75548377 None:intergenic 60.0%
TCGCCGGAAAAGATGCCCCC+GGG - chr1_1:75548352-75548371 Msa0044080:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 75547877 75550972 75547877 ID=Msa0044080;Name=Msa0044080
chr1_1 mRNA 75547877 75550972 75547877 ID=Msa0044080-mRNA-1;Parent=Msa0044080;Name=Msa0044080-mRNA-1;_AED=0.01;_eAED=0.01;_QI=139|1|1|1|1|1|2|581|243
chr1_1 exon 75547877 75548642 75547877 ID=Msa0044080-mRNA-1:exon:25492;Parent=Msa0044080-mRNA-1
chr1_1 exon 75550287 75550972 75550287 ID=Msa0044080-mRNA-1:exon:25491;Parent=Msa0044080-mRNA-1
chr1_1 five_prime_UTR 75550834 75550972 75550834 ID=Msa0044080-mRNA-1:five_prime_utr;Parent=Msa0044080-mRNA-1
chr1_1 CDS 75550287 75550833 75550287 ID=Msa0044080-mRNA-1:cds;Parent=Msa0044080-mRNA-1
chr1_1 CDS 75548458 75548642 75548458 ID=Msa0044080-mRNA-1:cds;Parent=Msa0044080-mRNA-1
chr1_1 three_prime_UTR 75547877 75548457 75547877 ID=Msa0044080-mRNA-1:three_prime_utr;Parent=Msa0044080-mRNA-1
Gene Sequence

>Msa0044080

ATGGGTGTAGGCATATCAGTGTTAATAGCACTAAAAGCAACAACCCTATTACTAATCTTCGCATACCTAAAAAACCTAGGCTTCTTAATTCTATCCATTCCATTCCTCTATTCATCCTTAGTCTCATTCCTCGTCTCAATCGCATCACACCCTTCCATCGATCTTCCCTTACTTCTCGGCAAAACCTCCGACGGAACATTCCCAATTGTTTCACTCATTATCTTCAGTCCTTATCTTTACTTCGTTCGAATCTTCTCCGCGCTTCGAAGACGAAAAAGCGGTGAACCACCTTATAGTAAGGTTCATGATGGACTTTACGTTGGTGGTTGGCCTTCTTCGCCGGAAAAGATGCCCCCGGGTGATCCTGCTATTATTGATTGTACTTGTGAGTTTCCGAGGTTGAAAAGTTTGAGTGGTGGTTTGCCTTATAGATGTGTTCCTACTTGGGATACTAGATCTCCTCAACCTGGTGATATTGAATTGGCTGTTAAATGGGCGCTTCGTAAGAGGGATCTTAATCGTCCCATTTTTGTTCATTGTGCTTATGGTCATGGAAGAAGTGTTGCTGTGATGTGTGCACTGTTAGTTGCACTAGGTGTCACAGATGATTGGAAAAATGCTGAAAAGCTGATAAAGCAAAAACGGCCCTACATTCGCATGAATGCTCTCCATCGCAAGGCACTTGAAGAATGGTCAGCGCATCGATTGTCTACGCCGAAGCCAAAGAAGTGA
Protein sequence

>Msa0044080

MGVGISVLIALKATTLLLIFAYLKNLGFLILSIPFLYSSLVSFLVSIASHPSIDLPLLLGKTSDGTFPIVSLIIFSPYLYFVRIFSALRRRKSGEPPYSKVHDGLYVGGWPSSPEKMPPGDPAIIDCTCEFPRLKSLSGGLPYRCVPTWDTRSPQPGDIELAVKWALRKRDLNRPIFVHCAYGHGRSVAVMCALLVALGVTDDWKNAEKLIKQKRPYIRMNALHRKALEEWSAHRLSTPKPKK*