AlfalfaGEDB | Msa0051250

M. sativa cultivar zhongmu-4 / Msa0051250

KEGG: -
GO: GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030312 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071944 GO:0080060 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051250	sp\|Q9FJV5\|KAN4_ARATH	47.176	301	111	9	21	316	17	274	1.42e-63	208

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051250	G7I588	95.098	306	3	2	11	316	1	294	0.0	583

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TFs/TRs:

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Gene ID	Type	Classification
Msa0051250	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002360	Msa0051250	0.965069	3.958786e-124	-8.615850e-47
Msa0051150	Msa0051250	0.956843	1.128703e-114	-8.615850e-47
Msa0051250	Msa0343410	0.964529	1.924636e-123	-8.615850e-47
Msa0051250	Msa0580690	0.917536	4.708864e-86	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051250	MtrunA17_Chr1g0154261	95.098	306	3	2	11	316	1	294	0.0	583
Msa0051250	MtrunA17_Chr3g0109981	46.193	197	79	5	136	307	268	462	1.80e-40	149
Msa0051250	MtrunA17_Chr5g0410741	44.792	192	76	7	136	300	213	401	9.71e-35	133
Msa0051250	MtrunA17_Chr8g0366281	84.722	72	11	0	137	208	168	239	1.89e-34	131
Msa0051250	MtrunA17_Chr6g0479431	82.857	70	12	0	137	206	197	266	8.76e-33	127
Msa0051250	MtrunA17_Chr8g0379121	61.538	78	27	2	129	204	96	172	1.39e-21	96.3
Msa0051250	MtrunA17_Chr7g0243081	57.692	78	31	1	126	201	53	130	2.47e-21	94.7
Msa0051250	MtrunA17_Chr2g0320231	64.789	71	22	2	136	204	89	158	6.70e-20	90.9
Msa0051250	MtrunA17_Chr1g0210911	59.091	66	25	1	135	198	13	78	9.75e-20	89.7
Msa0051250	MtrunA17_Chr1g0162931	59.701	67	26	1	132	198	9	74	1.84e-19	87.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051250	AT5G42630.1	47.176	301	111	9	21	316	17	274	1.44e-64	208
Msa0051250	AT5G42630.2	52.679	224	73	6	21	244	17	207	5.68e-53	176
Msa0051250	AT1G32240.2	61.290	124	27	3	85	208	177	279	1.00e-38	143
Msa0051250	AT1G32240.1	61.290	124	27	3	85	208	177	279	1.87e-38	143
Msa0051250	AT5G16560.1	46.907	194	70	8	136	302	213	400	6.04e-36	136
Msa0051250	AT4G17695.1	82.192	73	13	0	136	208	158	230	7.09e-35	132
Msa0051250	AT1G14600.1	57.500	80	32	1	123	200	2	81	9.85e-21	91.3
Msa0051250	AT2G42660.1	53.571	84	37	1	131	212	37	120	1.52e-20	90.9
Msa0051250	AT2G02060.2	60.870	69	25	1	129	195	14	82	4.81e-19	86.7
Msa0051250	AT2G02060.1	60.870	69	25	1	129	195	14	82	4.81e-19	86.7

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Find 127 sgRNAs with CRISPR-Local

Find 347 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCGCGGCCATAGATGTG+AGG	0.691470	1_2:+5342244	None:intergenic
AACCACTTCAAAATTCACTT+AGG	0.547130	1_2:-5340914	Msa0051250:intron
AAGGAACCTCAGTACTCCCC+AGG	0.587521	1_2:-5343113	Msa0051250:five_prime_UTR
AAGGAGAAACAGCCTGGGAA+TGG	0.545633	1_2:-5338108	Msa0051250:CDS
AATGAAGGAAGGAAGGAAGA+GGG	0.515503	1_2:+5342684	None:intergenic
AATGGGATACTGCAGACACA+AGG	0.640188	1_2:-5340999	Msa0051250:CDS
AATGTTGGGTCACGTTGATG+AGG	0.616823	1_2:+5342376	None:intergenic
ACAATCAGTTATTCATGACT+AGG	0.611201	1_2:-5337783	Msa0051250:CDS
ACACGTCAAGAGTCACTTAC+AGG	0.413830	1_2:-5341508	Msa0051250:intron
ACACTTGGAAGGCCTAATTG+GGG	0.657474	1_2:-5339070	Msa0051250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGATAATATTAGTAGCAG+TGG	+	chr1_2:5337829-5337848	None:intergenic	30.0%
AGCCAAAAGTTAGTGAAAAA+GGG	+	chr1_2:5337889-5337908	None:intergenic	30.0%
CTTAAATCAATTACCCTAGT+TGG	-	chr1_2:5338065-5338084	Msa0051250:intron	30.0%
CTTATGTTTAAAGATAGGTC+TGG	+	chr1_2:5338326-5338345	None:intergenic	30.0%
AAGTGCTCATGAAGAATAAT+AGG	+	chr1_2:5338782-5338801	None:intergenic	30.0%
CTCTCTGCAATAAATATCTA+AGG	-	chr1_2:5339118-5339137	Msa0051250:CDS	30.0%
GTTAGAGTTAATGAATGTGA+AGG	-	chr1_2:5339317-5339336	Msa0051250:CDS	30.0%
CTCATTAAACTGTAAGACTA+TGG	+	chr1_2:5339395-5339414	None:intergenic	30.0%
AACCACTTCAAAATTCACTT+AGG	-	chr1_2:5339947-5339966	Msa0051250:intron	30.0%
ATGTACTTGGAAATACCAAA+AGG	-	chr1_2:5340545-5340564	Msa0051250:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	5337735	5343148	5337735	ID=Msa0051250;Name=Msa0051250
chr1_2	mRNA	5337735	5343148	5337735	ID=Msa0051250-mRNA-1;Parent=Msa0051250;Name=Msa0051250-mRNA-1;_AED=0.24;_eAED=0.24;_QI=528\|0.62\|0.55\|0.66\|1\|1\|9\|0\|418
chr1_2	exon	5337735	5337867	5337735	ID=Msa0051250-mRNA-1:exon:1574;Parent=Msa0051250-mRNA-1
chr1_2	exon	5337970	5338034	5337970	ID=Msa0051250-mRNA-1:exon:1573;Parent=Msa0051250-mRNA-1
chr1_2	exon	5338076	5338184	5338076	ID=Msa0051250-mRNA-1:exon:1572;Parent=Msa0051250-mRNA-1
chr1_2	exon	5339028	5339152	5339028	ID=Msa0051250-mRNA-1:exon:1571;Parent=Msa0051250-mRNA-1
chr1_2	exon	5339303	5339402	5339303	ID=Msa0051250-mRNA-1:exon:1570;Parent=Msa0051250-mRNA-1
chr1_2	exon	5340915	5341023	5340915	ID=Msa0051250-mRNA-1:exon:1569;Parent=Msa0051250-mRNA-1
chr1_2	exon	5341219	5341258	5341219	ID=Msa0051250-mRNA-1:exon:1568;Parent=Msa0051250-mRNA-1
chr1_2	exon	5341509	5341585	5341509	ID=Msa0051250-mRNA-1:exon:1567;Parent=Msa0051250-mRNA-1
chr1_2	exon	5342122	5343148	5342122	ID=Msa0051250-mRNA-1:exon:1566;Parent=Msa0051250-mRNA-1
chr1_2	five_prime_UTR	5342621	5343148	5342621	ID=Msa0051250-mRNA-1:five_prime_utr;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5342122	5342620	5342122	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5341509	5341585	5341509	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5341219	5341258	5341219	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5340915	5341023	5340915	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5339303	5339402	5339303	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5339028	5339152	5339028	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5338076	5338184	5338076	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5337970	5338034	5337970	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1
chr1_2	CDS	5337735	5337867	5337735	ID=Msa0051250-mRNA-1:cds;Parent=Msa0051250-mRNA-1

Gene Sequence

>Msa0051250

ATGTTCACTAATAATTCTTCACATCCTAAAATGCATACTTTATTTTCACCTTTGTTAGAACCAGACCTATCTTTAAACATAAGTCTTCCTTCTAATATTTCTGATTCTGAACCCAAAGGAATAACAAAACCACTTTATAATGATAATATATGTTCAATATCAACTACTTCTGATTCAGCTAGTAGTGGAAGTGAGTTAAGCCATGAAAATCCCTTCATCTATCCTCATCAACGTGACCCAACATTGAGATTAGGCTTTGGAAATTCAGATTTGATGAATCCACATCATCATAATCATCACCTTCATCGTCATCAAGTGCAAGGTGTATCAAGAAACTTTAACCAACACTTTCAACCTCACATCTATGGCCGCGATTTCAAGCGAAATACAAGAGTCGTTAATGGTGTTAAAAGAAGTGTTAGAGCTCCAAGAATGAGATGGACTACAACCCTTCATTCCCATTTTGTTCACGCTGTTCAACTTCTTGGTGGTCATGAAAGAGCAACACCAAAATCTGTGTTAGAGTTAATGAATGTGAAGGATCTAACCCTAGCACACGTCAAGAGTCACTTACAGATGTATAGAACAGTGAAGACCACTGACAAATCAGGAGCAGGTAATGGGATACTGCAGACACAAGGGATTAATATTGTTCCTTTGCATGGAGCTAATTCCTCAGCTGATGAGAGACCAAATTTACCTCAACCACTTCAAAATTCACTTAGGACATCTTGGCAACCATCAATAGAGACAAATACAAACAATACTGAAGAAAAATCCGAAATTGGTTTGACGTATTCTAATTTGAAGGAAAATGATACCATGGTGAAGAGGTTGGATTCTGCCCAATTGACATGTTCAGACACTATGATTGACTTAGAATTCACACTTGGAAGGCCTAATTGGGGAAAAGACCATGCTGAATCATCAAGAGAATTAGCTCTTCTTAATATTCAATCAAATCAAGGTTTCGGTGGTGGATATATGCGATTTGGGGTGGGAGGAAGGAGAAACAGCCTGGGAATGGCGTCATCGGTTGTGGACTTGGGAGGAGGAGCTCATAATTCTACATATCAATGGCATTGGAGGCTAGATCCTAGTGGTGGTTATTCGGTTTGGGGCATACTTCTTCGGAGTAGGTTGCCAATGAAGGATAATTTGGTGGCGCGCGACATTCTTTCTCACGACAATCAGTTATTCATGACTAGGTGTGGAGGCTTCGAGACTGCTCAACATCTATTTCTGTTTTGCCCCTGA

Protein sequence

>Msa0051250

MFTNNSSHPKMHTLFSPLLEPDLSLNISLPSNISDSEPKGITKPLYNDNICSISTTSDSASSGSELSHENPFIYPHQRDPTLRLGFGNSDLMNPHHHNHHLHRHQVQGVSRNFNQHFQPHIYGRDFKRNTRVVNGVKRSVRAPRMRWTTTLHSHFVHAVQLLGGHERATPKSVLELMNVKDLTLAHVKSHLQMYRTVKTTDKSGAGNGILQTQGINIVPLHGANSSADERPNLPQPLQNSLRTSWQPSIETNTNNTEEKSEIGLTYSNLKENDTMVKRLDSAQLTCSDTMIDLEFTLGRPNWGKDHAESSRELALLNIQSNQGFGGGYMRFGVGGRRNSLGMASSVVDLGGGAHNSTYQWHWRLDPSGGYSVWGILLRSRLPMKDNLVARDILSHDNQLFMTRCGGFETAQHLFLFCP*