AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0057480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0057480 MtrunA17_Chr1g0147101 96.622 296 9 1 1 296 1 295 0.0 582
Msa0057480 MtrunA17_Chr1g0147091 82.492 297 44 3 1 296 1 290 1.47e-156 438
Msa0057480 MtrunA17_Chr1g0147081 79.264 299 48 3 1 295 1 289 4.12e-153 429
Msa0057480 MtrunA17_Chr6g0477351 73.438 128 22 3 10 136 28 144 1.56e-36 127
Msa0057480 MtrunA17_Chr3g0104601 24.684 158 83 2 139 296 21 142 2.30e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0057480 AT5G53400.1 59.930 287 99 2 25 296 19 304 1.46e-116 338
Msa0057480 AT4G27890.1 56.250 304 114 6 1 296 1 293 1.55e-109 319
Msa0057480 AT5G58740.1 25.949 158 81 3 139 296 21 142 9.04e-14 68.2

Find 100 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCATGATTTCCTGTTTC+TGG 0.058297 1_2:-13491080 None:intergenic
ACAGTTTGGGGTGTCTAAAT+TGG 0.188690 1_2:+13491400 Msa0057480:three_prime_UTR
TTATATATTTGAACATATTT+TGG 0.220188 1_2:+13491461 Msa0057480:three_prime_UTR
TAAAAGCTTACATCAATAAT+TGG 0.250303 1_2:-13490151 None:intergenic
AACTCTGGTTTATATATTCC+TGG 0.285035 1_2:+13491540 Msa0057480:three_prime_UTR
CAACCATTCATGTTTATGTT+TGG 0.295885 1_2:+13491361 Msa0057480:three_prime_UTR
CTCTCTTGAACTTTCTCTAA+TGG 0.315405 1_2:+13488497 Msa0057480:exon
ATGAGGTTTGGGTTTCTTCT+TGG 0.317575 1_2:-13488536 None:intergenic
TGATCAGAGGCAGAAGTCTA+TGG 0.321181 1_2:+13491035 Msa0057480:CDS
AGAAAGCTGCGCTATGATTC+GGG 0.325747 1_2:+13491257 Msa0057480:exon
TACACTCCCAAACAACAGTT+TGG 0.329221 1_2:+13491386 Msa0057480:three_prime_UTR
AGACACCCCAAACTGTTGTT+TGG 0.354071 1_2:-13491393 None:intergenic
TTCTGGAGCTCCTCACTTGT+TGG 0.356328 1_2:-13491063 None:intergenic
TTGGGTTTCTTCTTGGTAAT+CGG 0.361888 1_2:-13488529 None:intergenic
TCAAGCCTGATGAATGTTAC+TGG 0.367377 1_2:+13490360 Msa0057480:CDS
ATCCATACCATTACCCTGAT+TGG 0.375160 1_2:-13489993 None:intergenic
AACAGCAATCTTAGCTTTCT+CGG 0.384320 1_2:-13488757 None:intergenic
TTTGAGAAGAAGATGAGGTT+TGG 0.388546 1_2:-13488548 None:intergenic
GACACCCCAAACTGTTGTTT+GGG 0.392691 1_2:-13491392 None:intergenic
AAACATAAACATGAATGGTT+GGG 0.395039 1_2:-13491359 None:intergenic
TTGATGGACAGTTCCCAATC+AGG 0.395904 1_2:+13489979 Msa0057480:intron
TAGAAAGCTGCGCTATGATT+CGG 0.396170 1_2:+13491256 Msa0057480:exon
TCAACAGTCTGTCGAGTCTC+AGG 0.400798 1_2:-13490701 None:intergenic
TCACCAAGTTTGCTGCTCTC+AGG 0.401717 1_2:-13490671 None:intergenic
ACACTCCCAAACAACAGTTT+GGG 0.405028 1_2:+13491387 Msa0057480:three_prime_UTR
CAGTTTGGGGTGTCTAAATT+GGG 0.407050 1_2:+13491401 Msa0057480:three_prime_UTR
AGAAAAGCGGTTGGATTTGA+AGG 0.414688 1_2:-13488612 None:intergenic
TGGATCTGGAGAAATATTCT+TGG 0.418168 1_2:+13490011 Msa0057480:CDS
CATGAAGAAATTTATGAGTC+AGG 0.419859 1_2:+13491095 Msa0057480:CDS
CGATTTCTTCGATAATGATT+CGG 0.421929 1_2:+13488671 Msa0057480:CDS
TTGAGAAGAAGATGAGGTTT+GGG 0.427139 1_2:-13488547 None:intergenic
TTTAACCAAGCATGATCAAA+TGG 0.428418 1_2:+13490592 Msa0057480:CDS
AAGTGAGGAGCTCCAGAAAC+AGG 0.431963 1_2:+13491068 Msa0057480:CDS
TGAGAAGGAAGATGAGAAGA+CGG 0.436290 1_2:+13488851 Msa0057480:CDS
CATCTAAAAGTTGGAATCAA+AGG 0.443557 1_2:+13490121 Msa0057480:CDS
GAACCTGAGAGCAGCAAACT+TGG 0.444776 1_2:+13490668 Msa0057480:CDS
CCAGTCCATTTGATCATGCT+TGG 0.448786 1_2:-13490597 None:intergenic
AAGAAGAACCATCTAAAAGT+TGG 0.449134 1_2:+13490112 Msa0057480:CDS
GATGGAGAAAGTGGCTTAGC+AGG 0.450109 1_2:+13488873 Msa0057480:CDS
AGCTATGGAGGTAGATGAGA+AGG 0.453896 1_2:+13488836 Msa0057480:CDS
CGAATCATTATCGAAGAAAT+CGG 0.453970 1_2:-13488670 None:intergenic
TCAGGGTAATGGTATGGATC+TGG 0.458750 1_2:+13489997 Msa0057480:CDS
GGCGGCTGCTGTTGCTGCGG+CGG 0.461162 1_2:+13488806 Msa0057480:CDS
AAGGTGGAAGAGAACGGAAT+AGG 0.487694 1_2:-13488585 None:intergenic
TCCATACCATTACCCTGATT+GGG 0.488514 1_2:-13489992 None:intergenic
ATGCTCCAGTAACATTCATC+AGG 0.490196 1_2:-13490365 None:intergenic
TAAACCAGAGTTTGATTCGA+TGG 0.492391 1_2:-13491529 None:intergenic
AAACCAGAGTTTGATTCGAT+GGG 0.498271 1_2:-13491528 None:intergenic
CAAACATAAACATGAATGGT+TGG 0.500868 1_2:-13491360 None:intergenic
ATCTAAAAGTTGGAATCAAA+GGG 0.502699 1_2:+13490122 Msa0057480:CDS
CGATAATGATTCGGCTGAGA+AGG 0.502712 1_2:+13488680 Msa0057480:CDS
GGAGCTCCTCACTTGTTGGA+AGG 0.509413 1_2:-13491059 None:intergenic
AAGGAAGATGAGAAGACGGA+TGG 0.515138 1_2:+13488855 Msa0057480:CDS
TGGTGGAAATGTCTTGTTAA+GGG 0.518276 1_2:+13490617 Msa0057480:CDS
TCCCAATCAGGGTAATGGTA+TGG 0.518546 1_2:+13489991 Msa0057480:CDS
GCATTCGAGAAGTCCATCTC+CGG 0.524232 1_2:-13491223 None:intergenic
AGAGAAGGCGAAGGTTGATA+AGG 0.527997 1_2:+13488782 Msa0057480:CDS
TGTATTGCAGCATCCGGAGA+TGG 0.529847 1_2:+13491210 Msa0057480:intron
TCTGTCGAGTCTCAGGATCC+AGG 0.530996 1_2:-13490694 None:intergenic
TTATATCATGGTTTAAGTAA+CGG 0.532998 1_2:+13491488 Msa0057480:three_prime_UTR
AATCCAAACATAAACATGAA+TGG 0.536791 1_2:-13491364 None:intergenic
ATCCGCCGTTCGCGCTGTGA+AGG 0.537892 1_2:+13488710 Msa0057480:CDS
GAGAAGACGGATGGAGAAAG+TGG 0.540556 1_2:+13488864 Msa0057480:CDS
GTGAATGTTCCAGTGCCAAA+TGG 0.540930 1_2:+13490058 Msa0057480:CDS
TTCCCCATCGAATCAAACTC+TGG 0.541453 1_2:+13491525 Msa0057480:three_prime_UTR
TGATGGACAGTTCCCAATCA+GGG 0.541528 1_2:+13489980 Msa0057480:intron
TGCCAAATGGAACAAAATCA+AGG 0.549463 1_2:+13490071 Msa0057480:CDS
GAGCAGCAAACTTGGTGACC+TGG 0.549952 1_2:+13490676 Msa0057480:CDS
AACATAAACATGAATGGTTG+GGG 0.550301 1_2:-13491358 None:intergenic
AAGCTTACATCAATAATTGG+TGG 0.551582 1_2:-13490148 None:intergenic
ACAGTTCCCAATCAGGGTAA+TGG 0.556894 1_2:+13489986 Msa0057480:intron
GATCAGAGGCAGAAGTCTAT+GGG 0.558052 1_2:+13491036 Msa0057480:CDS
GATGAATGTTACTGGAGCAT+AGG 0.561295 1_2:+13490368 Msa0057480:CDS
CTGGTGGAAATGTCTTGTTA+AGG 0.563095 1_2:+13490616 Msa0057480:CDS
GGCGGCGGCTGCTGTTGCTG+CGG 0.565880 1_2:+13488803 Msa0057480:CDS
TGTATTCGACTTCATCGCTA+AGG 0.567639 1_2:+13488641 Msa0057480:CDS
TAAGATTGCTGTTGAAGAGA+AGG 0.574713 1_2:+13488767 Msa0057480:CDS
ACAGACTGTTGAAAAGATGA+TGG 0.576647 1_2:+13490712 Msa0057480:CDS
AGCATGATCAAATGGACTGG+TGG 0.576780 1_2:+13490600 Msa0057480:CDS
TGGATTTGAAGGATCGAAGG+TGG 0.582135 1_2:-13488601 None:intergenic
TTCTTTAACAGTTTGATCAG+AGG 0.586849 1_2:+13491022 Msa0057480:intron
TACTCTAAAGATTACCCCAA+AGG 0.588595 1_2:-13491313 None:intergenic
TAATGATTCGGCTGAGAAGG+TGG 0.597727 1_2:+13488683 Msa0057480:CDS
CCAAGCATGATCAAATGGAC+TGG 0.599013 1_2:+13490597 Msa0057480:CDS
GGCGAAGGTTGATAAGGCGG+CGG 0.599571 1_2:+13488788 Msa0057480:CDS
TGCTGTTGAAGAGAAGGCGA+AGG 0.601660 1_2:+13488773 Msa0057480:CDS
GGATCGAAGGTGGAAGAGAA+CGG 0.602076 1_2:-13488591 None:intergenic
GAAAGCTGCGCTATGATTCG+GGG 0.610589 1_2:+13491258 Msa0057480:exon
GGTTGGATTTGAAGGATCGA+AGG 0.613022 1_2:-13488604 None:intergenic
AAAGCTGCGCTATGATTCGG+GGG 0.616383 1_2:+13491259 Msa0057480:three_prime_UTR
TACCTTCACAGCGCGAACGG+CGG 0.622007 1_2:-13488712 None:intergenic
CTTTACCTTCACAGCGCGAA+CGG 0.628341 1_2:-13488715 None:intergenic
CACTCCCAAACAACAGTTTG+GGG 0.650363 1_2:+13491388 Msa0057480:three_prime_UTR
AACCAGAGTTTGATTCGATG+GGG 0.651671 1_2:-13491527 None:intergenic
GAAGGCGAAGGTTGATAAGG+CGG 0.663476 1_2:+13488785 Msa0057480:CDS
TGTGAAGGTAAAGAAAGCTA+AGG 0.664999 1_2:+13488725 Msa0057480:CDS
TGCGGCGGAGAAGAAAGCTA+TGG 0.665095 1_2:+13488821 Msa0057480:CDS
TATGGGCCTTCCAACAAGTG+AGG 0.673276 1_2:+13491053 Msa0057480:CDS
GGCGGAGAAGAAAGCTATGG+AGG 0.684045 1_2:+13488824 Msa0057480:CDS
AAGCTGCGCTATGATTCGGG+GGG 0.755475 1_2:+13491260 Msa0057480:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTTTTTAAAAATAT+TGG + chr1_2:13488953-13488972 Msa0057480:intron 0.0%
!!! TTAATTTTTTTAAAAATATT+GGG + chr1_2:13488954-13488973 Msa0057480:intron 0.0%
!! AAAGAAATAAAAAAAAACTA+GGG - chr1_2:13488936-13488955 None:intergenic 10.0%
!! TAAAGAAATAAAAAAAAACT+AGG - chr1_2:13488937-13488956 None:intergenic 10.0%
!!! TTATATATTTGAACATATTT+TGG + chr1_2:13491461-13491480 Msa0057480:three_prime_UTR 10.0%
!! TTGATTGTTTATTAAATCTA+TGG + chr1_2:13489334-13489353 Msa0057480:intron 15.0%
!! ATTGTAAATTATCAAAATGA+TGG + chr1_2:13489798-13489817 Msa0057480:intron 15.0%
!! AAAAAATTCAGATATATAGT+TGG - chr1_2:13489924-13489943 None:intergenic 15.0%
!!! TCATTATTTTAAAATTTCTC+AGG + chr1_2:13490319-13490338 Msa0057480:intron 15.0%
!!! CATTATTTTAAAATTTCTCA+GGG + chr1_2:13490320-13490339 Msa0057480:intron 15.0%
!!! ATTATTTTAAAATTTCTCAG+GGG + chr1_2:13490321-13490340 Msa0057480:intron 15.0%
!!! TTTTAAACTTGTGAAAATTT+TGG + chr1_2:13490471-13490490 Msa0057480:intron 15.0%
!!! AAAATATTTTGAAATACCTT+TGG + chr1_2:13491297-13491316 Msa0057480:three_prime_UTR 15.0%
!!! AAATATTTTGAAATACCTTT+GGG + chr1_2:13491298-13491317 Msa0057480:three_prime_UTR 15.0%
!!! ATTTTGTTTTATTTTTATGC+AGG - chr1_2:13491568-13491587 None:intergenic 15.0%
!! TGAAATTTATGAGATAACAA+TGG + chr1_2:13489023-13489042 Msa0057480:intron 20.0%
!! GAAATTTATGAGATAACAAT+GGG + chr1_2:13489024-13489043 Msa0057480:intron 20.0%
!!! AAATTATCAAAATGATGGAA+TGG + chr1_2:13489803-13489822 Msa0057480:intron 20.0%
!! TTTAACTATTCATTAGCTAA+TGG + chr1_2:13489871-13489890 Msa0057480:intron 20.0%
!! TAAAAGCTTACATCAATAAT+TGG - chr1_2:13490154-13490173 None:intergenic 20.0%
!! TTAAAATAATGAATCAGCAA+AGG - chr1_2:13490311-13490330 None:intergenic 20.0%
!!! TTATTTTAAAATTTCTCAGG+GGG + chr1_2:13490322-13490341 Msa0057480:intron 20.0%
!!! TACTTAATATAACTTGCATT+TGG + chr1_2:13490769-13490788 Msa0057480:intron 20.0%
!!! TATGTATCTATTCTTTTGTT+GGG + chr1_2:13490840-13490859 Msa0057480:intron 20.0%
!!! AGATTTAAATAACACTGTTT+TGG + chr1_2:13490956-13490975 Msa0057480:intron 20.0%
!!! AATATTTTGAAATACCTTTG+GGG + chr1_2:13491299-13491318 Msa0057480:three_prime_UTR 20.0%
!!! TATTTTGGAGTTTTATATCA+TGG + chr1_2:13491476-13491495 Msa0057480:three_prime_UTR 20.0%
!! TTATATCATGGTTTAAGTAA+CGG + chr1_2:13491488-13491507 Msa0057480:three_prime_UTR 20.0%
! TGTATCATATTGTATTGCTA+TGG + chr1_2:13489094-13489113 Msa0057480:intron 25.0%
! TTAAATATGCCAAAATTGCT+AGG + chr1_2:13489197-13489216 Msa0057480:intron 25.0%
! TAAATATGCCAAAATTGCTA+GGG + chr1_2:13489198-13489217 Msa0057480:intron 25.0%
! TAGATGATAAACCAATGATA+AGG + chr1_2:13489380-13489399 Msa0057480:intron 25.0%
!! CACTCAATGCTTAAAATTTT+GGG - chr1_2:13489441-13489460 None:intergenic 25.0%
!! ACACTCAATGCTTAAAATTT+TGG - chr1_2:13489442-13489461 None:intergenic 25.0%
!!! TATGCATATACCACATTTTT+TGG + chr1_2:13489520-13489539 Msa0057480:intron 25.0%
! GTTTCTATTACCAAAAAATG+TGG - chr1_2:13489533-13489552 None:intergenic 25.0%
! ATCTATATCACAAAGACTTT+CGG + chr1_2:13489567-13489586 Msa0057480:intron 25.0%
! ATCTAAAAGTTGGAATCAAA+GGG + chr1_2:13490122-13490141 Msa0057480:CDS 25.0%
! TCAAACAAACTAAAAATTCC+AGG - chr1_2:13490275-13490294 None:intergenic 25.0%
! GAATTATGGTCACTTAAAAA+GGG - chr1_2:13490426-13490445 None:intergenic 25.0%
! TGAATTATGGTCACTTAAAA+AGG - chr1_2:13490427-13490446 None:intergenic 25.0%
! TTAAGTGACCATAATTCATT+AGG + chr1_2:13490429-13490448 Msa0057480:intron 25.0%
! ATCATGATCCTAATGAATTA+TGG - chr1_2:13490440-13490459 None:intergenic 25.0%
! ATAATTCATTAGGATCATGA+TGG + chr1_2:13490439-13490458 Msa0057480:intron 25.0%
!!! TTTAAAACCTATTTTTGCAG+AGG + chr1_2:13490547-13490566 Msa0057480:intron 25.0%
!! GTATGTATCTATTCTTTTGT+TGG + chr1_2:13490839-13490858 Msa0057480:intron 25.0%
! ATAATCGAATAGAGATAACT+TGG + chr1_2:13490901-13490920 Msa0057480:intron 25.0%
! TCAAACTGTTAAAGAATACA+GGG - chr1_2:13491018-13491037 None:intergenic 25.0%
! ATCAAACTGTTAAAGAATAC+AGG - chr1_2:13491019-13491038 None:intergenic 25.0%
! AGAAGCAATACAAAAATCAA+AGG - chr1_2:13491125-13491144 None:intergenic 25.0%
! AAACATAAACATGAATGGTT+GGG - chr1_2:13491362-13491381 None:intergenic 25.0%
! AATCCAAACATAAACATGAA+TGG - chr1_2:13491367-13491386 None:intergenic 25.0%
!! TTTTGTTTGAGAAGAAGATG+AGG - chr1_2:13488556-13488575 None:intergenic 30.0%
!! GAATACATTTTCGAGAAAAG+CGG - chr1_2:13488628-13488647 None:intergenic 30.0%
CGAATCATTATCGAAGAAAT+CGG - chr1_2:13488673-13488692 None:intergenic 30.0%
CGATTTCTTCGATAATGATT+CGG + chr1_2:13488671-13488690 Msa0057480:CDS 30.0%
! TTTGCTATGAACATTGTGTT+GGG + chr1_2:13488994-13489013 Msa0057480:intron 30.0%
!! GCAATTTTGGCATATTTAAC+CGG - chr1_2:13489196-13489215 None:intergenic 30.0%
AAATATGCCAAAATTGCTAG+GGG + chr1_2:13489199-13489218 Msa0057480:intron 30.0%
GATGAAATGACAATAATCAC+CGG - chr1_2:13489256-13489275 None:intergenic 30.0%
AAAATGTGGTATATGCATAG+TGG - chr1_2:13489519-13489538 None:intergenic 30.0%
! TTTGTGATATAGATGGACTT+AGG - chr1_2:13489561-13489580 None:intergenic 30.0%
GAAAGTCTTTGTGATATAGA+TGG - chr1_2:13489568-13489587 None:intergenic 30.0%
AAACACAATCAAATCAGAGA+GGG - chr1_2:13489754-13489773 None:intergenic 30.0%
! TACATCTTTGCTCTTTTTGA+TGG + chr1_2:13489963-13489982 Msa0057480:intron 30.0%
! AACCTTGATTTTGTTCCATT+TGG - chr1_2:13490076-13490095 None:intergenic 30.0%
AAGAAGAACCATCTAAAAGT+TGG + chr1_2:13490112-13490131 Msa0057480:CDS 30.0%
! CTTTGATTCCAACTTTTAGA+TGG - chr1_2:13490123-13490142 None:intergenic 30.0%
CATCTAAAAGTTGGAATCAA+AGG + chr1_2:13490121-13490140 Msa0057480:CDS 30.0%
AAGCTTACATCAATAATTGG+TGG - chr1_2:13490151-13490170 None:intergenic 30.0%
TTTAACCAAGCATGATCAAA+TGG + chr1_2:13490592-13490611 Msa0057480:CDS 30.0%
!!! ACTTTTTGGGTGTTAATTTC+AGG - chr1_2:13490647-13490666 None:intergenic 30.0%
GATACATACTTAAATGCAAC+AGG - chr1_2:13490828-13490847 None:intergenic 30.0%
! AAGATGACATTCTAGTTGAA+TGG + chr1_2:13490875-13490894 Msa0057480:intron 30.0%
!! ATAACACTGTTTTGGATTTG+AGG + chr1_2:13490964-13490983 Msa0057480:intron 30.0%
TTCTTTAACAGTTTGATCAG+AGG + chr1_2:13491022-13491041 Msa0057480:intron 30.0%
CATGAAGAAATTTATGAGTC+AGG + chr1_2:13491095-13491114 Msa0057480:CDS 30.0%
CAAATTTACTAGATCGACAA+AGG - chr1_2:13491172-13491191 None:intergenic 30.0%
! ATTTTATGTATTGCAGCATC+CGG + chr1_2:13491204-13491223 Msa0057480:intron 30.0%
!!! TTAGAGTACTTGTTTTGACT+TGG + chr1_2:13491328-13491347 Msa0057480:three_prime_UTR 30.0%
AACATAAACATGAATGGTTG+GGG - chr1_2:13491361-13491380 None:intergenic 30.0%
CAAACATAAACATGAATGGT+TGG - chr1_2:13491363-13491382 None:intergenic 30.0%
CAACCATTCATGTTTATGTT+TGG + chr1_2:13491361-13491380 Msa0057480:three_prime_UTR 30.0%
!! GTTTTGTTCACAAACATGTT+TGG + chr1_2:13491423-13491442 Msa0057480:three_prime_UTR 30.0%
! AACTCTGGTTTATATATTCC+TGG + chr1_2:13491540-13491559 Msa0057480:three_prime_UTR 30.0%
!!! TTTATTTTTATGCAGGAACC+AGG - chr1_2:13491561-13491580 None:intergenic 30.0%
CTCTCTTGAACTTTCTCTAA+TGG + chr1_2:13488497-13488516 Msa0057480:exon 35.0%
! TTGGGTTTCTTCTTGGTAAT+CGG - chr1_2:13488532-13488551 None:intergenic 35.0%
TTGAGAAGAAGATGAGGTTT+GGG - chr1_2:13488550-13488569 None:intergenic 35.0%
TTTGAGAAGAAGATGAGGTT+TGG - chr1_2:13488551-13488570 None:intergenic 35.0%
TGTGAAGGTAAAGAAAGCTA+AGG + chr1_2:13488725-13488744 Msa0057480:CDS 35.0%
! AACAGCAATCTTAGCTTTCT+CGG - chr1_2:13488760-13488779 None:intergenic 35.0%
! TAAGATTGCTGTTGAAGAGA+AGG + chr1_2:13488767-13488786 Msa0057480:CDS 35.0%
!! GTTTGCTATGAACATTGTGT+TGG + chr1_2:13488993-13489012 Msa0057480:intron 35.0%
! GTTTAAACCCCTAGCAATTT+TGG - chr1_2:13489209-13489228 None:intergenic 35.0%
CAAACACAATCAAATCAGAG+AGG - chr1_2:13489755-13489774 None:intergenic 35.0%
! CTCTCTGATTTGATTGTGTT+TGG + chr1_2:13489753-13489772 Msa0057480:intron 35.0%
TGGATCTGGAGAAATATTCT+TGG + chr1_2:13490011-13490030 Msa0057480:CDS 35.0%
TGCCAAATGGAACAAAATCA+AGG + chr1_2:13490071-13490090 Msa0057480:CDS 35.0%
CAACAGTAACTGCTATAACT+TGG - chr1_2:13490177-13490196 None:intergenic 35.0%
ATAACACAAACAAGTGTCAC+TGG - chr1_2:13490214-13490233 None:intergenic 35.0%
AGAGTCTTCTGATTGTAACT+AGG + chr1_2:13490497-13490516 Msa0057480:intron 35.0%
! TGGTGGAAATGTCTTGTTAA+GGG + chr1_2:13490617-13490636 Msa0057480:CDS 35.0%
ACAGACTGTTGAAAAGATGA+TGG + chr1_2:13490712-13490731 Msa0057480:CDS 35.0%
TCTTCATGATTTCCTGTTTC+TGG - chr1_2:13491083-13491102 None:intergenic 35.0%
! TACTCTAAAGATTACCCCAA+AGG - chr1_2:13491316-13491335 None:intergenic 35.0%
! AAACCAGAGTTTGATTCGAT+GGG - chr1_2:13491531-13491550 None:intergenic 35.0%
! TAAACCAGAGTTTGATTCGA+TGG - chr1_2:13491532-13491551 None:intergenic 35.0%
! ATGAGGTTTGGGTTTCTTCT+TGG - chr1_2:13488539-13488558 None:intergenic 40.0%
!! GAGAACGGAATAGGTTTTGA+GGG - chr1_2:13488579-13488598 None:intergenic 40.0%
! AGAGAACGGAATAGGTTTTG+AGG - chr1_2:13488580-13488599 None:intergenic 40.0%
AGAAAAGCGGTTGGATTTGA+AGG - chr1_2:13488615-13488634 None:intergenic 40.0%
!! ACATTTTCGAGAAAAGCGGT+TGG - chr1_2:13488624-13488643 None:intergenic 40.0%
TGTATTCGACTTCATCGCTA+AGG + chr1_2:13488641-13488660 Msa0057480:CDS 40.0%
TGAGAAGGAAGATGAGAAGA+CGG + chr1_2:13488851-13488870 Msa0057480:CDS 40.0%
!! TCCACAATTTCTAGCTCAAC+CGG + chr1_2:13489174-13489193 Msa0057480:intron 40.0%
TGATTCACGGTCCTTATCAT+TGG - chr1_2:13489394-13489413 None:intergenic 40.0%
TAACGCGATCTCTTGATTCA+CGG - chr1_2:13489407-13489426 None:intergenic 40.0%
! ATATAGATGGACTTAGGCAG+GGG - chr1_2:13489555-13489574 None:intergenic 40.0%
! GATATAGATGGACTTAGGCA+GGG - chr1_2:13489556-13489575 None:intergenic 40.0%
! TGATATAGATGGACTTAGGC+AGG - chr1_2:13489557-13489576 None:intergenic 40.0%
ATCCATAGTTTGGACTGACA+GGG - chr1_2:13489627-13489646 None:intergenic 40.0%
ATGGTACCAGATCCATAGTT+TGG - chr1_2:13489637-13489656 None:intergenic 40.0%
ATGGATCTGGTACCATATCT+TGG + chr1_2:13489641-13489660 Msa0057480:intron 40.0%
TGGATCTGGTACCATATCTT+GGG + chr1_2:13489642-13489661 Msa0057480:intron 40.0%
CGTCGCTTCTTGCATTTAAA+TGG - chr1_2:13489696-13489715 None:intergenic 40.0%
TCCATACCATTACCCTGATT+GGG - chr1_2:13489995-13490014 None:intergenic 40.0%
ATCCATACCATTACCCTGAT+TGG - chr1_2:13489996-13490015 None:intergenic 40.0%
! GATTTTGTTCCATTTGGCAC+TGG - chr1_2:13490070-13490089 None:intergenic 40.0%
AATTCCAGGCTAAAGACAGA+AGG - chr1_2:13490261-13490280 None:intergenic 40.0%
TCAAGCCTGATGAATGTTAC+TGG + chr1_2:13490360-13490379 Msa0057480:CDS 40.0%
ATGCTCCAGTAACATTCATC+AGG - chr1_2:13490368-13490387 None:intergenic 40.0%
GATGAATGTTACTGGAGCAT+AGG + chr1_2:13490368-13490387 Msa0057480:CDS 40.0%
CTCTGATCCTCTGCAAAAAT+AGG - chr1_2:13490557-13490576 None:intergenic 40.0%
! CTGGTGGAAATGTCTTGTTA+AGG + chr1_2:13490616-13490635 Msa0057480:CDS 40.0%
! GCTCTCAGGTTCAACTTTTT+GGG - chr1_2:13490660-13490679 None:intergenic 40.0%
! TGCTCTCAGGTTCAACTTTT+TGG - chr1_2:13490661-13490680 None:intergenic 40.0%
TTGGATTTGAGGATTTCTCC+CGG + chr1_2:13490975-13490994 Msa0057480:intron 40.0%
GCAACATTAAACCAACTACC+GGG - chr1_2:13490996-13491015 None:intergenic 40.0%
GGCAACATTAAACCAACTAC+CGG - chr1_2:13490997-13491016 None:intergenic 40.0%
TAGAAAGCTGCGCTATGATT+CGG + chr1_2:13491256-13491275 Msa0057480:exon 40.0%
TACACTCCCAAACAACAGTT+TGG + chr1_2:13491386-13491405 Msa0057480:three_prime_UTR 40.0%
ACACTCCCAAACAACAGTTT+GGG + chr1_2:13491387-13491406 Msa0057480:three_prime_UTR 40.0%
! ACAGTTTGGGGTGTCTAAAT+TGG + chr1_2:13491400-13491419 Msa0057480:three_prime_UTR 40.0%
! CAGTTTGGGGTGTCTAAATT+GGG + chr1_2:13491401-13491420 Msa0057480:three_prime_UTR 40.0%
! AACCAGAGTTTGATTCGATG+GGG - chr1_2:13491530-13491549 None:intergenic 40.0%
AAGGTGGAAGAGAACGGAAT+AGG - chr1_2:13488588-13488607 None:intergenic 45.0%
TGGATTTGAAGGATCGAAGG+TGG - chr1_2:13488604-13488623 None:intergenic 45.0%
GGTTGGATTTGAAGGATCGA+AGG - chr1_2:13488607-13488626 None:intergenic 45.0%
! CGATAATGATTCGGCTGAGA+AGG + chr1_2:13488680-13488699 Msa0057480:CDS 45.0%
! TAATGATTCGGCTGAGAAGG+TGG + chr1_2:13488683-13488702 Msa0057480:CDS 45.0%
!! AGAGAAGGCGAAGGTTGATA+AGG + chr1_2:13488782-13488801 Msa0057480:CDS 45.0%
AGCTATGGAGGTAGATGAGA+AGG + chr1_2:13488836-13488855 Msa0057480:CDS 45.0%
AAGGAAGATGAGAAGACGGA+TGG + chr1_2:13488855-13488874 Msa0057480:CDS 45.0%
ACCGGTTGAGCTAGAAATTG+TGG - chr1_2:13489178-13489197 None:intergenic 45.0%
GATCCATAGTTTGGACTGAC+AGG - chr1_2:13489628-13489647 None:intergenic 45.0%
GTCAGTCCAAACTATGGATC+TGG + chr1_2:13489628-13489647 Msa0057480:intron 45.0%
GGATCTGGTACCATATCTTG+GGG + chr1_2:13489643-13489662 Msa0057480:intron 45.0%
GATCTGGTACCATATCTTGG+GGG + chr1_2:13489644-13489663 Msa0057480:intron 45.0%
ACCATATCTTGGGGGAATTC+AGG + chr1_2:13489652-13489671 Msa0057480:intron 45.0%
CCATATCTTGGGGGAATTCA+GGG + chr1_2:13489653-13489672 Msa0057480:intron 45.0%
! TTGATGGACAGTTCCCAATC+AGG + chr1_2:13489979-13489998 Msa0057480:intron 45.0%
! TGATGGACAGTTCCCAATCA+GGG + chr1_2:13489980-13489999 Msa0057480:intron 45.0%
ACAGTTCCCAATCAGGGTAA+TGG + chr1_2:13489986-13490005 Msa0057480:intron 45.0%
! TCCCAATCAGGGTAATGGTA+TGG + chr1_2:13489991-13490010 Msa0057480:CDS 45.0%
! TCAGGGTAATGGTATGGATC+TGG + chr1_2:13489997-13490016 Msa0057480:CDS 45.0%
GTGAATGTTCCAGTGCCAAA+TGG + chr1_2:13490058-13490077 Msa0057480:CDS 45.0%
! CACAAACAAGTGTCACTGGA+AGG - chr1_2:13490210-13490229 None:intergenic 45.0%
CCAGTCCATTTGATCATGCT+TGG - chr1_2:13490600-13490619 None:intergenic 45.0%
CCAAGCATGATCAAATGGAC+TGG + chr1_2:13490597-13490616 Msa0057480:CDS 45.0%
!! AGCATGATCAAATGGACTGG+TGG + chr1_2:13490600-13490619 Msa0057480:CDS 45.0%
TGATCAGAGGCAGAAGTCTA+TGG + chr1_2:13491035-13491054 Msa0057480:CDS 45.0%
GATCAGAGGCAGAAGTCTAT+GGG + chr1_2:13491036-13491055 Msa0057480:CDS 45.0%
AGAAAGCTGCGCTATGATTC+GGG + chr1_2:13491257-13491276 Msa0057480:exon 45.0%
!!! TATGATTCGGGGGGCTTTTT+TGG + chr1_2:13491269-13491288 Msa0057480:three_prime_UTR 45.0%
CACTCCCAAACAACAGTTTG+GGG + chr1_2:13491388-13491407 Msa0057480:three_prime_UTR 45.0%
! GACACCCCAAACTGTTGTTT+GGG - chr1_2:13491395-13491414 None:intergenic 45.0%
AGACACCCCAAACTGTTGTT+TGG - chr1_2:13491396-13491415 None:intergenic 45.0%
TTCCCCATCGAATCAAACTC+TGG + chr1_2:13491525-13491544 Msa0057480:three_prime_UTR 45.0%
GGATCGAAGGTGGAAGAGAA+CGG - chr1_2:13488594-13488613 None:intergenic 50.0%
CTTTACCTTCACAGCGCGAA+CGG - chr1_2:13488718-13488737 None:intergenic 50.0%
TGCTGTTGAAGAGAAGGCGA+AGG + chr1_2:13488773-13488792 Msa0057480:CDS 50.0%
!! GAAGGCGAAGGTTGATAAGG+CGG + chr1_2:13488785-13488804 Msa0057480:CDS 50.0%
GAGAAGACGGATGGAGAAAG+TGG + chr1_2:13488864-13488883 Msa0057480:CDS 50.0%
GATGGAGAAAGTGGCTTAGC+AGG + chr1_2:13488873-13488892 Msa0057480:CDS 50.0%
AAACCCACACACACGCAAGT+CGG - chr1_2:13489233-13489252 None:intergenic 50.0%
CTCCCTGTCAGTCCAAACTA+TGG + chr1_2:13489622-13489641 Msa0057480:intron 50.0%
CCCTGAATTCCCCCAAGATA+TGG - chr1_2:13489656-13489675 None:intergenic 50.0%
CGTTCCTTCTGTCTTTAGCC+TGG + chr1_2:13490254-13490273 Msa0057480:intron 50.0%
GAACCTGAGAGCAGCAAACT+TGG + chr1_2:13490668-13490687 Msa0057480:CDS 50.0%
!! TCACCAAGTTTGCTGCTCTC+AGG - chr1_2:13490674-13490693 None:intergenic 50.0%
TCAACAGTCTGTCGAGTCTC+AGG - chr1_2:13490704-13490723 None:intergenic 50.0%
TGAGGATTTCTCCCGGTAGT+TGG + chr1_2:13490982-13491001 Msa0057480:intron 50.0%
TATGGGCCTTCCAACAAGTG+AGG + chr1_2:13491053-13491072 Msa0057480:CDS 50.0%
!! TTCTGGAGCTCCTCACTTGT+TGG - chr1_2:13491066-13491085 None:intergenic 50.0%
AAGTGAGGAGCTCCAGAAAC+AGG + chr1_2:13491068-13491087 Msa0057480:CDS 50.0%
TGTATTGCAGCATCCGGAGA+TGG + chr1_2:13491210-13491229 Msa0057480:intron 50.0%
GCATTCGAGAAGTCCATCTC+CGG - chr1_2:13491226-13491245 None:intergenic 50.0%
GAAAGCTGCGCTATGATTCG+GGG + chr1_2:13491258-13491277 Msa0057480:exon 50.0%
! AAAGCTGCGCTATGATTCGG+GGG + chr1_2:13491259-13491278 Msa0057480:three_prime_UTR 50.0%
TGCGGCGGAGAAGAAAGCTA+TGG + chr1_2:13488821-13488840 Msa0057480:CDS 55.0%
GGCGGAGAAGAAAGCTATGG+AGG + chr1_2:13488824-13488843 Msa0057480:CDS 55.0%
AACCCACACACACGCAAGTC+GGG - chr1_2:13489232-13489251 None:intergenic 55.0%
CTTGCGTGTGTGTGGGTTTC+CGG + chr1_2:13489234-13489253 Msa0057480:intron 55.0%
! GAGCAGCAAACTTGGTGACC+TGG + chr1_2:13490676-13490695 Msa0057480:CDS 55.0%
TCTGTCGAGTCTCAGGATCC+AGG - chr1_2:13490697-13490716 None:intergenic 55.0%
! GGAGCTCCTCACTTGTTGGA+AGG - chr1_2:13491062-13491081 None:intergenic 55.0%
! AAGCTGCGCTATGATTCGGG+GGG + chr1_2:13491260-13491279 Msa0057480:three_prime_UTR 55.0%
TACCTTCACAGCGCGAACGG+CGG - chr1_2:13488715-13488734 None:intergenic 60.0%
!! GGCGAAGGTTGATAAGGCGG+CGG + chr1_2:13488788-13488807 Msa0057480:CDS 60.0%
! AACCCCGACTTGCGTGTGTG+TGG + chr1_2:13489226-13489245 Msa0057480:intron 60.0%
! ACCCCGACTTGCGTGTGTGT+GGG + chr1_2:13489227-13489246 Msa0057480:intron 60.0%
ACCCACACACACGCAAGTCG+GGG - chr1_2:13489231-13489250 None:intergenic 60.0%
ATCCGCCGTTCGCGCTGTGA+AGG + chr1_2:13488710-13488729 Msa0057480:CDS 65.0%
GGCGGCGGCTGCTGTTGCTG+CGG + chr1_2:13488803-13488822 Msa0057480:CDS 75.0%
GGCGGCTGCTGTTGCTGCGG+CGG + chr1_2:13488806-13488825 Msa0057480:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 13488449 13491632 13488449 ID=Msa0057480;Name=Msa0057480
chr1_2 mRNA 13488449 13491632 13488449 ID=Msa0057480-mRNA-1;Parent=Msa0057480;Name=Msa0057480-mRNA-1;_AED=0.27;_eAED=0.27;_QI=67|1|1|1|1|1|6|374|296
chr1_2 exon 13488449 13488894 13488449 ID=Msa0057480-mRNA-1:exon:5304;Parent=Msa0057480-mRNA-1
chr1_2 exon 13489990 13490162 13489990 ID=Msa0057480-mRNA-1:exon:5305;Parent=Msa0057480-mRNA-1
chr1_2 exon 13490341 13490389 13490341 ID=Msa0057480-mRNA-1:exon:5306;Parent=Msa0057480-mRNA-1
chr1_2 exon 13490567 13490733 13490567 ID=Msa0057480-mRNA-1:exon:5307;Parent=Msa0057480-mRNA-1
chr1_2 exon 13491033 13491116 13491033 ID=Msa0057480-mRNA-1:exon:5308;Parent=Msa0057480-mRNA-1
chr1_2 exon 13491220 13491632 13491220 ID=Msa0057480-mRNA-1:exon:5309;Parent=Msa0057480-mRNA-1
chr1_2 five_prime_UTR 13488449 13488515 13488449 ID=Msa0057480-mRNA-1:five_prime_utr;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13488516 13488894 13488516 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13489990 13490162 13489990 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13490341 13490389 13490341 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13490567 13490733 13490567 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13491033 13491116 13491033 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 CDS 13491220 13491258 13491220 ID=Msa0057480-mRNA-1:cds;Parent=Msa0057480-mRNA-1
chr1_2 three_prime_UTR 13491259 13491632 13491259 ID=Msa0057480-mRNA-1:three_prime_utr;Parent=Msa0057480-mRNA-1
Gene Sequence

>Msa0057480

ATGGCGATTATCTCCGATTACCAAGAAGAAACCCAAACCTCATCTTCTTCTCAAACAAAACCCTCAAAACCTATTCCGTTCTCTTCCACCTTCGATCCTTCAAATCCAACCGCTTTTCTCGAAAATGTATTCGACTTCATCGCTAAGGAAAGTACCGATTTCTTCGATAATGATTCGGCTGAGAAGGTGGTTTTATCCGCCGTTCGCGCTGTGAAGGTAAAGAAAGCTAAGGCTGTTGCTGCCGAGAAAGCTAAGATTGCTGTTGAAGAGAAGGCGAAGGTTGATAAGGCGGCGGCTGCTGTTGCTGCGGCGGAGAAGAAAGCTATGGAGGTAGATGAGAAGGAAGATGAGAAGACGGATGGAGAAAGTGGCTTAGCAGTTCCCAATCAGGGTAATGGTATGGATCTGGAGAAATATTCTTGGACTCAAACTCTTCAAGAGCTCAATGTGAATGTTCCAGTGCCAAATGGAACAAAATCAAGGTTTGTCACATGTGAGATAAAGAAGAACCATCTAAAAGTTGGAATCAAAGGGCAGCCACCAATTATTGATGGGGAGCTGTATAAATCTATCAAGCCTGATGAATGTTACTGGAGCATAGAGGATCAGAGTACACTTTCTATTCTTTTAACCAAGCATGATCAAATGGACTGGTGGAAATGTCTTGTTAAGGGTGATCCTGAAATTAACACCCAAAAAGTTGAACCTGAGAGCAGCAAACTTGGTGACCTGGATCCTGAGACTCGACAGACTGTTGAAAAGATGATGTTTGATCAGAGGCAGAAGTCTATGGGCCTTCCAACAAGTGAGGAGCTCCAGAAACAGGAAATCATGAAGAAATTTATGAGTCAGCATCCGGAGATGGACTTCTCGAATGCAAAATTATCTTAG
Protein sequence

>Msa0057480

MAIISDYQEETQTSSSSQTKPSKPIPFSSTFDPSNPTAFLENVFDFIAKESTDFFDNDSAEKVVLSAVRAVKVKKAKAVAAEKAKIAVEEKAKVDKAAAAVAAAEKKAMEVDEKEDEKTDGESGLAVPNQGNGMDLEKYSWTQTLQELNVNVPVPNGTKSRFVTCEIKKNHLKVGIKGQPPIIDGELYKSIKPDECYWSIEDQSTLSILLTKHDQMDWWKCLVKGDPEINTQKVEPESSKLGDLDPETRQTVEKMMFDQRQKSMGLPTSEELQKQEIMKKFMSQHPEMDFSNAKLS*