AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0063500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0063500 MtrunA17_Chr1g0164821 100.000 48 0 0 1 48 1 48 6.91e-33 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTAGTGTTCTTTGTTTA+AGG 0.102484 1_2:+24069110 Msa0063500:three_prime_UTR
TTTCCATGCAAGTTTATATT+TGG 0.142980 1_2:+24068934 Msa0063500:CDS
TTGATGTCTTCGACACTTTC+AGG 0.157889 1_2:+24068995 Msa0063500:exon
TTTGCAGCATCAACAGTTTC+TGG 0.205539 1_2:+24068908 Msa0063500:intron
AGGATTATATGTAAAATCTT+TGG 0.207833 1_2:+24069130 Msa0063500:three_prime_UTR
AATTGTGGTCTGCAATATAA+AGG 0.246223 1_2:+24069444 Msa0063500:three_prime_UTR
ACTCTCAACATTACTCTTTC+CGG 0.250688 1_2:-24069256 None:intergenic
AGACTTTGAAACAACATCTT+AGG 0.255184 1_2:-24069631 None:intergenic
CCCATCCTGGTTGGAATGTT+GGG 0.286175 1_2:-24068463 None:intergenic
GGATTATATGTAAAATCTTT+GGG 0.309419 1_2:+24069131 Msa0063500:three_prime_UTR
ATGTGGCATGAATTTCTTCT+AGG 0.316936 1_2:-24068487 None:intergenic
TAAGGCTATTCATCATTATA+TGG 0.325082 1_2:-24068424 None:intergenic
TAATGATGAATAGCCTTATC+TGG 0.349011 1_2:+24068429 Msa0063500:exon
ATGTTGAGAGTTGTTGCTTT+TGG 0.365055 1_2:+24069268 Msa0063500:three_prime_UTR
GTCCATGATTAAAGTGTTGA+TGG 0.365851 1_2:+24069174 Msa0063500:three_prime_UTR
TTCCATCAACACTTTAATCA+TGG 0.378693 1_2:-24069176 None:intergenic
ATTGTGGTCTGCAATATAAA+GGG 0.383944 1_2:+24069445 Msa0063500:three_prime_UTR
AGGAAAGAAGCTTTGGTTGT+TGG 0.396242 1_2:+24069040 Msa0063500:three_prime_UTR
TCCCATCCTGGTTGGAATGT+TGG 0.400822 1_2:-24068464 None:intergenic
TGTTGATGGAAAGGTTTAGT+TGG 0.408545 1_2:+24069188 Msa0063500:three_prime_UTR
AGACATCAACGCGTCGAAAC+CGG 0.430436 1_2:-24068981 None:intergenic
CAAATGCATAAGTTGGCAAA+TGG 0.438197 1_2:+24068514 Msa0063500:CDS
CTTCTAGGTCCCATCCTGGT+TGG 0.462181 1_2:-24068472 None:intergenic
AAAACGATGGTTTAAAATTG+TGG 0.467219 1_2:+24069429 Msa0063500:three_prime_UTR
AAAGAAGCTTTGGTTGTTGG+AGG 0.477083 1_2:+24069043 Msa0063500:three_prime_UTR
TAAAATCTTTGGGGAGAATG+TGG 0.478812 1_2:+24069141 Msa0063500:three_prime_UTR
AATTTGTGCAATGGCTTATG+TGG 0.507413 1_2:+24069342 Msa0063500:three_prime_UTR
AATCCAAATATAAACTTGCA+TGG 0.517522 1_2:-24068937 None:intergenic
TGATTAAAGTGTTGATGGAA+AGG 0.521545 1_2:+24069179 Msa0063500:three_prime_UTR
AAAATCTTTGGGGAGAATGT+GGG 0.532039 1_2:+24069142 Msa0063500:three_prime_UTR
GCGTCGAAACCGGTATAATA+CGG 0.533596 1_2:-24068971 None:intergenic
ATTGTGTTACCGTATTATAC+CGG 0.533901 1_2:+24068962 Msa0063500:CDS
AAGAAGCTTTGGTTGTTGGA+GGG 0.534987 1_2:+24069044 Msa0063500:three_prime_UTR
CCCAACATTCCAACCAGGAT+GGG 0.536926 1_2:+24068463 Msa0063500:CDS
TTTGCTTCATGTTCTCAAAG+AGG 0.537931 1_2:+24069309 Msa0063500:three_prime_UTR
ATTTCTTCTAGGTCCCATCC+TGG 0.540010 1_2:-24068476 None:intergenic
AACTAGTGAAGTACAAAGGT+TGG 0.543343 1_2:+24069068 Msa0063500:three_prime_UTR
TGTGTGTAGGAAAGAAGCTT+TGG 0.547839 1_2:+24069033 Msa0063500:three_prime_UTR
TTTGATTGCAAAGTCACCAC+CGG 0.550429 1_2:+24069237 Msa0063500:three_prime_UTR
TTGTCACATACATAATGGTA+AGG 0.559614 1_2:-24068563 None:intergenic
ACCCAACATTCCAACCAGGA+TGG 0.567280 1_2:+24068462 Msa0063500:CDS
AAATCTTTGGGGAGAATGTG+GGG 0.568787 1_2:+24069143 Msa0063500:three_prime_UTR
TTGGCAAATGGAAGAAACAA+TGG 0.577416 1_2:+24068526 Msa0063500:CDS
ATGTAGTCAAATTTGTGCAA+TGG 0.580987 1_2:+24069333 Msa0063500:three_prime_UTR
CTCAACATTACTCTTTCCGG+TGG 0.610843 1_2:-24069253 None:intergenic
GATTATATGTAAAATCTTTG+GGG 0.628144 1_2:+24069132 Msa0063500:three_prime_UTR
AGTATGTAATATCAAAACGA+TGG 0.650160 1_2:+24069416 Msa0063500:three_prime_UTR
ACGAAGGTGACTTTGAGAAG+CGG 0.658196 1_2:-24069528 None:intergenic
AATGTGCAACATGTGTGTGT+AGG 0.666637 1_2:+24069020 Msa0063500:three_prime_UTR
GGGAAACTAGTGAAGTACAA+AGG 0.675405 1_2:+24069064 Msa0063500:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTTTATTTATGCAAT+TGG - chr1_2:24069400-24069419 None:intergenic 15.0%
!! TGAAAACAAAAATAGATTTA+AGG + chr1_2:24069569-24069588 Msa0063500:three_prime_UTR 15.0%
!!! TACTTTTTTATTTATGCAAT+TGG - chr1_2:24069400-24069419 None:intergenic 15.0%
!! TGAAAACAAAAATAGATTTA+AGG + chr1_2:24069569-24069588 Msa0063500:three_prime_UTR 15.0%
!! ATGAATATTATCTCAAGAAA+AGG + chr1_2:24068677-24068696 Msa0063500:intron 20.0%
!!! AAAAGATTTTTTTTACCTTC+TGG + chr1_2:24068722-24068741 Msa0063500:intron 20.0%
!! TATAATCTTAATCTTTGCAA+AGG + chr1_2:24068763-24068782 Msa0063500:intron 20.0%
!! ATAATCTTAATCTTTGCAAA+GGG + chr1_2:24068764-24068783 Msa0063500:intron 20.0%
!! AGGATTATATGTAAAATCTT+TGG + chr1_2:24069130-24069149 Msa0063500:three_prime_UTR 20.0%
!! GGATTATATGTAAAATCTTT+GGG + chr1_2:24069131-24069150 Msa0063500:three_prime_UTR 20.0%
!! GATTATATGTAAAATCTTTG+GGG + chr1_2:24069132-24069151 Msa0063500:three_prime_UTR 20.0%
!! ATGAATATTATCTCAAGAAA+AGG + chr1_2:24068677-24068696 Msa0063500:intron 20.0%
!!! AAAAGATTTTTTTTACCTTC+TGG + chr1_2:24068722-24068741 Msa0063500:intron 20.0%
!! TATAATCTTAATCTTTGCAA+AGG + chr1_2:24068763-24068782 Msa0063500:intron 20.0%
!! ATAATCTTAATCTTTGCAAA+GGG + chr1_2:24068764-24068783 Msa0063500:intron 20.0%
!! AGGATTATATGTAAAATCTT+TGG + chr1_2:24069130-24069149 Msa0063500:three_prime_UTR 20.0%
!! GGATTATATGTAAAATCTTT+GGG + chr1_2:24069131-24069150 Msa0063500:three_prime_UTR 20.0%
!! GATTATATGTAAAATCTTTG+GGG + chr1_2:24069132-24069151 Msa0063500:three_prime_UTR 20.0%
! TAAGGCTATTCATCATTATA+TGG - chr1_2:24068427-24068446 None:intergenic 25.0%
! ACTTATGCATTTGAAAAATG+TGG - chr1_2:24068507-24068526 None:intergenic 25.0%
!!! CATTTTTCAAATGCATAAGT+TGG + chr1_2:24068507-24068526 Msa0063500:CDS 25.0%
!!! AAGTTTTGTCACATACATAA+TGG - chr1_2:24068571-24068590 None:intergenic 25.0%
! TGGAAAGATGATAATTTGAA+GGG - chr1_2:24068595-24068614 None:intergenic 25.0%
! ATGGAAAGATGATAATTTGA+AGG - chr1_2:24068596-24068615 None:intergenic 25.0%
! ATGAAATTGTTGTAAAGAGA+AGG - chr1_2:24068631-24068650 None:intergenic 25.0%
! ATTATAAGTGGAAGAGATAT+AGG - chr1_2:24068749-24068768 None:intergenic 25.0%
! GCAAAGATTAAGATTATAAG+TGG - chr1_2:24068761-24068780 None:intergenic 25.0%
! ATAATCAGCTTCATTATCAA+AGG - chr1_2:24068867-24068886 None:intergenic 25.0%
!! TGATAATGAAGCTGATTATA+AGG + chr1_2:24068868-24068887 Msa0063500:intron 25.0%
! TTTCCATGCAAGTTTATATT+TGG + chr1_2:24068934-24068953 Msa0063500:CDS 25.0%
! AATCCAAATATAAACTTGCA+TGG - chr1_2:24068940-24068959 None:intergenic 25.0%
!! TTGTTAGTGTTCTTTGTTTA+AGG + chr1_2:24069110-24069129 Msa0063500:three_prime_UTR 25.0%
!!! TAGTTGGATTTTGATAAGTT+TGG + chr1_2:24069204-24069223 Msa0063500:three_prime_UTR 25.0%
! AGTATGTAATATCAAAACGA+TGG + chr1_2:24069416-24069435 Msa0063500:three_prime_UTR 25.0%
!! AAAACGATGGTTTAAAATTG+TGG + chr1_2:24069429-24069448 Msa0063500:three_prime_UTR 25.0%
! TAAGGCTATTCATCATTATA+TGG - chr1_2:24068427-24068446 None:intergenic 25.0%
! ACTTATGCATTTGAAAAATG+TGG - chr1_2:24068507-24068526 None:intergenic 25.0%
!!! CATTTTTCAAATGCATAAGT+TGG + chr1_2:24068507-24068526 Msa0063500:CDS 25.0%
!!! AAGTTTTGTCACATACATAA+TGG - chr1_2:24068571-24068590 None:intergenic 25.0%
! TGGAAAGATGATAATTTGAA+GGG - chr1_2:24068595-24068614 None:intergenic 25.0%
! ATGGAAAGATGATAATTTGA+AGG - chr1_2:24068596-24068615 None:intergenic 25.0%
! ATGAAATTGTTGTAAAGAGA+AGG - chr1_2:24068631-24068650 None:intergenic 25.0%
! ATTATAAGTGGAAGAGATAT+AGG - chr1_2:24068749-24068768 None:intergenic 25.0%
! GCAAAGATTAAGATTATAAG+TGG - chr1_2:24068761-24068780 None:intergenic 25.0%
! ATAATCAGCTTCATTATCAA+AGG - chr1_2:24068867-24068886 None:intergenic 25.0%
!! TGATAATGAAGCTGATTATA+AGG + chr1_2:24068868-24068887 Msa0063500:intron 25.0%
! TTTCCATGCAAGTTTATATT+TGG + chr1_2:24068934-24068953 Msa0063500:CDS 25.0%
! AATCCAAATATAAACTTGCA+TGG - chr1_2:24068940-24068959 None:intergenic 25.0%
!! TTGTTAGTGTTCTTTGTTTA+AGG + chr1_2:24069110-24069129 Msa0063500:three_prime_UTR 25.0%
!!! TAGTTGGATTTTGATAAGTT+TGG + chr1_2:24069204-24069223 Msa0063500:three_prime_UTR 25.0%
! AGTATGTAATATCAAAACGA+TGG + chr1_2:24069416-24069435 Msa0063500:three_prime_UTR 25.0%
!! AAAACGATGGTTTAAAATTG+TGG + chr1_2:24069429-24069448 Msa0063500:three_prime_UTR 25.0%
!! TAATGATGAATAGCCTTATC+TGG + chr1_2:24068429-24068448 Msa0063500:exon 30.0%
TTGTCACATACATAATGGTA+AGG - chr1_2:24068566-24068585 None:intergenic 30.0%
! ATTTTCCCACTTTCTTGTAT+TGG + chr1_2:24068803-24068822 Msa0063500:intron 30.0%
ATTGTGTTACCGTATTATAC+CGG + chr1_2:24068962-24068981 Msa0063500:CDS 30.0%
TTCCATCAACACTTTAATCA+TGG - chr1_2:24069179-24069198 None:intergenic 30.0%
!! TGATTAAAGTGTTGATGGAA+AGG + chr1_2:24069179-24069198 Msa0063500:three_prime_UTR 30.0%
ATGTAGTCAAATTTGTGCAA+TGG + chr1_2:24069333-24069352 Msa0063500:three_prime_UTR 30.0%
AATTGTGGTCTGCAATATAA+AGG + chr1_2:24069444-24069463 Msa0063500:three_prime_UTR 30.0%
ATTGTGGTCTGCAATATAAA+GGG + chr1_2:24069445-24069464 Msa0063500:three_prime_UTR 30.0%
!! AGACTTTGAAACAACATCTT+AGG - chr1_2:24069634-24069653 None:intergenic 30.0%
!! TAATGATGAATAGCCTTATC+TGG + chr1_2:24068429-24068448 Msa0063500:exon 30.0%
TTGTCACATACATAATGGTA+AGG - chr1_2:24068566-24068585 None:intergenic 30.0%
! ATTTTCCCACTTTCTTGTAT+TGG + chr1_2:24068803-24068822 Msa0063500:intron 30.0%
ATTGTGTTACCGTATTATAC+CGG + chr1_2:24068962-24068981 Msa0063500:CDS 30.0%
TTCCATCAACACTTTAATCA+TGG - chr1_2:24069179-24069198 None:intergenic 30.0%
!! TGATTAAAGTGTTGATGGAA+AGG + chr1_2:24069179-24069198 Msa0063500:three_prime_UTR 30.0%
ATGTAGTCAAATTTGTGCAA+TGG + chr1_2:24069333-24069352 Msa0063500:three_prime_UTR 30.0%
AATTGTGGTCTGCAATATAA+AGG + chr1_2:24069444-24069463 Msa0063500:three_prime_UTR 30.0%
ATTGTGGTCTGCAATATAAA+GGG + chr1_2:24069445-24069464 Msa0063500:three_prime_UTR 30.0%
!! AGACTTTGAAACAACATCTT+AGG - chr1_2:24069634-24069653 None:intergenic 30.0%
!!! GTTGGAATGTTGGGTTTTTT+GGG - chr1_2:24068457-24068476 None:intergenic 35.0%
ATGTGGCATGAATTTCTTCT+AGG - chr1_2:24068490-24068509 None:intergenic 35.0%
! CAAATGCATAAGTTGGCAAA+TGG + chr1_2:24068514-24068533 Msa0063500:CDS 35.0%
TTGGCAAATGGAAGAAACAA+TGG + chr1_2:24068526-24068545 Msa0063500:CDS 35.0%
GAGAAGGACTAAGATATCAA+TGG - chr1_2:24068615-24068634 None:intergenic 35.0%
AGAACCAATAGAAGAATTGC+TGG - chr1_2:24068830-24068849 None:intergenic 35.0%
AACTAGTGAAGTACAAAGGT+TGG + chr1_2:24069068-24069087 Msa0063500:three_prime_UTR 35.0%
! TAAAATCTTTGGGGAGAATG+TGG + chr1_2:24069141-24069160 Msa0063500:three_prime_UTR 35.0%
! AAAATCTTTGGGGAGAATGT+GGG + chr1_2:24069142-24069161 Msa0063500:three_prime_UTR 35.0%
!! GTCCATGATTAAAGTGTTGA+TGG + chr1_2:24069174-24069193 Msa0063500:three_prime_UTR 35.0%
! TGTTGATGGAAAGGTTTAGT+TGG + chr1_2:24069188-24069207 Msa0063500:three_prime_UTR 35.0%
ACTCTCAACATTACTCTTTC+CGG - chr1_2:24069259-24069278 None:intergenic 35.0%
!!! ATGTTGAGAGTTGTTGCTTT+TGG + chr1_2:24069268-24069287 Msa0063500:three_prime_UTR 35.0%
TTTGCTTCATGTTCTCAAAG+AGG + chr1_2:24069309-24069328 Msa0063500:three_prime_UTR 35.0%
AATTTGTGCAATGGCTTATG+TGG + chr1_2:24069342-24069361 Msa0063500:three_prime_UTR 35.0%
TGTGGCACAAAAATTGAGAT+TGG + chr1_2:24069360-24069379 Msa0063500:three_prime_UTR 35.0%
! CTGCAATATAAAGGGTTTTG+AGG + chr1_2:24069453-24069472 Msa0063500:three_prime_UTR 35.0%
!! GTCGTTTTAAATTGTGACGA+AGG - chr1_2:24069547-24069566 None:intergenic 35.0%
!!! GTTGGAATGTTGGGTTTTTT+GGG - chr1_2:24068457-24068476 None:intergenic 35.0%
ATGTGGCATGAATTTCTTCT+AGG - chr1_2:24068490-24068509 None:intergenic 35.0%
! CAAATGCATAAGTTGGCAAA+TGG + chr1_2:24068514-24068533 Msa0063500:CDS 35.0%
TTGGCAAATGGAAGAAACAA+TGG + chr1_2:24068526-24068545 Msa0063500:CDS 35.0%
GAGAAGGACTAAGATATCAA+TGG - chr1_2:24068615-24068634 None:intergenic 35.0%
AGAACCAATAGAAGAATTGC+TGG - chr1_2:24068830-24068849 None:intergenic 35.0%
AACTAGTGAAGTACAAAGGT+TGG + chr1_2:24069068-24069087 Msa0063500:three_prime_UTR 35.0%
! TAAAATCTTTGGGGAGAATG+TGG + chr1_2:24069141-24069160 Msa0063500:three_prime_UTR 35.0%
! AAAATCTTTGGGGAGAATGT+GGG + chr1_2:24069142-24069161 Msa0063500:three_prime_UTR 35.0%
!! GTCCATGATTAAAGTGTTGA+TGG + chr1_2:24069174-24069193 Msa0063500:three_prime_UTR 35.0%
! TGTTGATGGAAAGGTTTAGT+TGG + chr1_2:24069188-24069207 Msa0063500:three_prime_UTR 35.0%
ACTCTCAACATTACTCTTTC+CGG - chr1_2:24069259-24069278 None:intergenic 35.0%
!!! ATGTTGAGAGTTGTTGCTTT+TGG + chr1_2:24069268-24069287 Msa0063500:three_prime_UTR 35.0%
TTTGCTTCATGTTCTCAAAG+AGG + chr1_2:24069309-24069328 Msa0063500:three_prime_UTR 35.0%
AATTTGTGCAATGGCTTATG+TGG + chr1_2:24069342-24069361 Msa0063500:three_prime_UTR 35.0%
TGTGGCACAAAAATTGAGAT+TGG + chr1_2:24069360-24069379 Msa0063500:three_prime_UTR 35.0%
! CTGCAATATAAAGGGTTTTG+AGG + chr1_2:24069453-24069472 Msa0063500:three_prime_UTR 35.0%
!! GTCGTTTTAAATTGTGACGA+AGG - chr1_2:24069547-24069566 None:intergenic 35.0%
!!! GGTTGGAATGTTGGGTTTTT+TGG - chr1_2:24068458-24068477 None:intergenic 40.0%
AAAAACCCAACATTCCAACC+AGG + chr1_2:24068458-24068477 Msa0063500:CDS 40.0%
CTGGTCCAATACAAGAAAGT+GGG - chr1_2:24068811-24068830 None:intergenic 40.0%
TGGACCAGCAATTCTTCTAT+TGG + chr1_2:24068823-24068842 Msa0063500:intron 40.0%
TTTGCAGCATCAACAGTTTC+TGG + chr1_2:24068908-24068927 Msa0063500:intron 40.0%
TTGATGTCTTCGACACTTTC+AGG + chr1_2:24068995-24069014 Msa0063500:exon 40.0%
AATGTGCAACATGTGTGTGT+AGG + chr1_2:24069020-24069039 Msa0063500:three_prime_UTR 40.0%
TGTGTGTAGGAAAGAAGCTT+TGG + chr1_2:24069033-24069052 Msa0063500:three_prime_UTR 40.0%
! AGGAAAGAAGCTTTGGTTGT+TGG + chr1_2:24069040-24069059 Msa0063500:three_prime_UTR 40.0%
! AAAGAAGCTTTGGTTGTTGG+AGG + chr1_2:24069043-24069062 Msa0063500:three_prime_UTR 40.0%
! AAGAAGCTTTGGTTGTTGGA+GGG + chr1_2:24069044-24069063 Msa0063500:three_prime_UTR 40.0%
GGGAAACTAGTGAAGTACAA+AGG + chr1_2:24069064-24069083 Msa0063500:three_prime_UTR 40.0%
! AAATCTTTGGGGAGAATGTG+GGG + chr1_2:24069143-24069162 Msa0063500:three_prime_UTR 40.0%
TTTGATTGCAAAGTCACCAC+CGG + chr1_2:24069237-24069256 Msa0063500:three_prime_UTR 40.0%
!!! TTGAGAGTTGTTGCTTTTGG+AGG + chr1_2:24069271-24069290 Msa0063500:three_prime_UTR 40.0%
!!! GGTTGGAATGTTGGGTTTTT+TGG - chr1_2:24068458-24068477 None:intergenic 40.0%
AAAAACCCAACATTCCAACC+AGG + chr1_2:24068458-24068477 Msa0063500:CDS 40.0%
CTGGTCCAATACAAGAAAGT+GGG - chr1_2:24068811-24068830 None:intergenic 40.0%
TGGACCAGCAATTCTTCTAT+TGG + chr1_2:24068823-24068842 Msa0063500:intron 40.0%
TTTGCAGCATCAACAGTTTC+TGG + chr1_2:24068908-24068927 Msa0063500:intron 40.0%
TTGATGTCTTCGACACTTTC+AGG + chr1_2:24068995-24069014 Msa0063500:exon 40.0%
AATGTGCAACATGTGTGTGT+AGG + chr1_2:24069020-24069039 Msa0063500:three_prime_UTR 40.0%
TGTGTGTAGGAAAGAAGCTT+TGG + chr1_2:24069033-24069052 Msa0063500:three_prime_UTR 40.0%
! AGGAAAGAAGCTTTGGTTGT+TGG + chr1_2:24069040-24069059 Msa0063500:three_prime_UTR 40.0%
! AAAGAAGCTTTGGTTGTTGG+AGG + chr1_2:24069043-24069062 Msa0063500:three_prime_UTR 40.0%
! AAGAAGCTTTGGTTGTTGGA+GGG + chr1_2:24069044-24069063 Msa0063500:three_prime_UTR 40.0%
GGGAAACTAGTGAAGTACAA+AGG + chr1_2:24069064-24069083 Msa0063500:three_prime_UTR 40.0%
! AAATCTTTGGGGAGAATGTG+GGG + chr1_2:24069143-24069162 Msa0063500:three_prime_UTR 40.0%
TTTGATTGCAAAGTCACCAC+CGG + chr1_2:24069237-24069256 Msa0063500:three_prime_UTR 40.0%
!!! TTGAGAGTTGTTGCTTTTGG+AGG + chr1_2:24069271-24069290 Msa0063500:three_prime_UTR 40.0%
!!! GGTTTTTTGGGAGCCAGATA+AGG - chr1_2:24068445-24068464 None:intergenic 45.0%
ATTTCTTCTAGGTCCCATCC+TGG - chr1_2:24068479-24068498 None:intergenic 45.0%
GGAAGAGATATAGGACCAGA+AGG - chr1_2:24068740-24068759 None:intergenic 45.0%
GCTGGTCCAATACAAGAAAG+TGG - chr1_2:24068812-24068831 None:intergenic 45.0%
GCGTCGAAACCGGTATAATA+CGG - chr1_2:24068974-24068993 None:intergenic 45.0%
CTCAACATTACTCTTTCCGG+TGG - chr1_2:24069256-24069275 None:intergenic 45.0%
! ACGAAGGTGACTTTGAGAAG+CGG - chr1_2:24069531-24069550 None:intergenic 45.0%
!!! GGTTTTTTGGGAGCCAGATA+AGG - chr1_2:24068445-24068464 None:intergenic 45.0%
ATTTCTTCTAGGTCCCATCC+TGG - chr1_2:24068479-24068498 None:intergenic 45.0%
GGAAGAGATATAGGACCAGA+AGG - chr1_2:24068740-24068759 None:intergenic 45.0%
GCTGGTCCAATACAAGAAAG+TGG - chr1_2:24068812-24068831 None:intergenic 45.0%
GCGTCGAAACCGGTATAATA+CGG - chr1_2:24068974-24068993 None:intergenic 45.0%
CTCAACATTACTCTTTCCGG+TGG - chr1_2:24069256-24069275 None:intergenic 45.0%
! ACGAAGGTGACTTTGAGAAG+CGG - chr1_2:24069531-24069550 None:intergenic 45.0%
ACCCAACATTCCAACCAGGA+TGG + chr1_2:24068462-24068481 Msa0063500:CDS 50.0%
CCCATCCTGGTTGGAATGTT+GGG - chr1_2:24068466-24068485 None:intergenic 50.0%
CCCAACATTCCAACCAGGAT+GGG + chr1_2:24068463-24068482 Msa0063500:CDS 50.0%
TCCCATCCTGGTTGGAATGT+TGG - chr1_2:24068467-24068486 None:intergenic 50.0%
AGACATCAACGCGTCGAAAC+CGG - chr1_2:24068984-24069003 None:intergenic 50.0%
ACCCAACATTCCAACCAGGA+TGG + chr1_2:24068462-24068481 Msa0063500:CDS 50.0%
CCCATCCTGGTTGGAATGTT+GGG - chr1_2:24068466-24068485 None:intergenic 50.0%
CCCAACATTCCAACCAGGAT+GGG + chr1_2:24068463-24068482 Msa0063500:CDS 50.0%
TCCCATCCTGGTTGGAATGT+TGG - chr1_2:24068467-24068486 None:intergenic 50.0%
AGACATCAACGCGTCGAAAC+CGG - chr1_2:24068984-24069003 None:intergenic 50.0%
CTTCTAGGTCCCATCCTGGT+TGG - chr1_2:24068475-24068494 None:intergenic 55.0%
CTTCTAGGTCCCATCCTGGT+TGG - chr1_2:24068475-24068494 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 24068406 24069722 24068406 ID=Msa0063500;Name=Msa0063500
chr1_2 mRNA 24068406 24069722 24068406 ID=Msa0063500-mRNA-1;Parent=Msa0063500;Name=Msa0063500-mRNA-1;_AED=0.13;_eAED=0.13;_QI=25|1|1|1|0|0.5|2|724|75
chr1_2 exon 24068406 24068574 24068406 ID=Msa0063500-mRNA-1:exon:8641;Parent=Msa0063500-mRNA-1
chr1_2 exon 24068915 24069722 24068915 ID=Msa0063500-mRNA-1:exon:8642;Parent=Msa0063500-mRNA-1
chr1_2 five_prime_UTR 24068406 24068430 24068406 ID=Msa0063500-mRNA-1:five_prime_utr;Parent=Msa0063500-mRNA-1
chr1_2 CDS 24068431 24068574 24068431 ID=Msa0063500-mRNA-1:cds;Parent=Msa0063500-mRNA-1
chr1_2 CDS 24068915 24068998 24068915 ID=Msa0063500-mRNA-1:cds;Parent=Msa0063500-mRNA-1
chr1_2 three_prime_UTR 24068999 24069722 24068999 ID=Msa0063500-mRNA-1:three_prime_utr;Parent=Msa0063500-mRNA-1
Gene Sequence

>Msa0063500

ATGATGAATAGCCTTATCTGGCTCCCAAAAAACCCAACATTCCAACCAGGATGGGACCTAGAAGAAATTCATGCCACATTTTTCAAATGCATAAGTTGGCAAATGGAAGAAACAATGGATGCAATCAATTGTCCTTACCATTATCATCAACAGTTTCTGGAATTTTCCATGCAAGTTTATATTTGGATTCAATTGTGTTACCGTATTATACCGGTTTCGACGCGTTGA
Protein sequence

>Msa0063500

MMNSLIWLPKNPTFQPGWDLEEIHATFFKCISWQMEETMDAINCPYHYHQQFLEFSMQVYIWIQLCYRIIPVSTR*