AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0154320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154320 MtrunA17_Chr1g0164821 100.000 48 0 0 1 48 1 48 1.34e-32 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTAGTGTTCTTTGTTTA+AGG 0.102484 1_4:+18835095 Msa0154320:three_prime_UTR
TTTGCATGCAAGTTTATATT+TGG 0.149495 1_4:+18834919 Msa0154320:CDS
CTGATGTCTTCGACACTTTC+AGG 0.178221 1_4:+18834980 Msa0154320:exon
CCAACTTATGCATTTGAAAA+AGG 0.179068 1_4:-18834492 None:intergenic
TTTGCAGCATCAACAGTTTC+TGG 0.205539 1_4:+18834893 Msa0154320:intron
AGGATTATATGTAAAATCTT+TGG 0.207833 1_4:+18835115 Msa0154320:three_prime_UTR
AGACTTTAAAACAGCATCTT+AGG 0.230749 1_4:-18835611 None:intergenic
AATTGTGGTATGCAATATAA+AGG 0.236352 1_4:+18835429 Msa0154320:three_prime_UTR
ACTCTCAACATTACTCTTTC+CGG 0.250688 1_4:-18835241 None:intergenic
CCCATCCAGGTTGGAATGTT+GGG 0.289826 1_4:-18834448 None:intergenic
GGATTATATGTAAAATCTTT+GGG 0.309419 1_4:+18835116 Msa0154320:three_prime_UTR
TAAGGCTATTCATCATTATA+TGG 0.325082 1_4:-18834409 None:intergenic
TAATGATGAATAGCCTTATC+TGG 0.349011 1_4:+18834414 Msa0154320:exon
ATGTTGAGAGTTGTTGCTTT+TGG 0.365055 1_4:+18835253 Msa0154320:three_prime_UTR
GTCCATGATTAAAGTGTTGA+TGG 0.365851 1_4:+18835159 Msa0154320:three_prime_UTR
ATTGTGGTATGCAATATAAA+GGG 0.378206 1_4:+18835430 Msa0154320:three_prime_UTR
TTCCATCAACACTTTAATCA+TGG 0.378693 1_4:-18835161 None:intergenic
AGGTGGCATGAATTTCTTCT+AGG 0.385813 1_4:-18834472 None:intergenic
AGGAAAGAAGCTTTGGTTGT+TGG 0.396242 1_4:+18835025 Msa0154320:three_prime_UTR
TGTTGATGGAAAGGTTTAGT+TGG 0.408545 1_4:+18835173 Msa0154320:three_prime_UTR
AGACATCAGTGCGTCGAAAC+CGG 0.424412 1_4:-18834966 None:intergenic
TCCCATCCAGGTTGGAATGT+TGG 0.427040 1_4:-18834449 None:intergenic
CAAATGCATAAGTTGGCAAA+TGG 0.438197 1_4:+18834499 Msa0154320:CDS
AAAACGATGGTTTAAAATTG+TGG 0.467219 1_4:+18835414 Msa0154320:three_prime_UTR
AAAGAAGCTTTGGTTGTTGG+AGG 0.477083 1_4:+18835028 Msa0154320:three_prime_UTR
TAAAATCTTTGGGGAGAATG+TGG 0.478812 1_4:+18835126 Msa0154320:three_prime_UTR
AATTTGTGCAATGGCTTATG+TGG 0.510079 1_4:+18835327 Msa0154320:three_prime_UTR
CTTCTAGGTCCCATCCAGGT+TGG 0.514155 1_4:-18834457 None:intergenic
CCCAACATTCCAACCTGGAT+GGG 0.516887 1_4:+18834448 Msa0154320:CDS
ACCCAACATTCCAACCTGGA+TGG 0.518422 1_4:+18834447 Msa0154320:CDS
TGATTAAAGTGTTGATGGAA+AGG 0.521545 1_4:+18835164 Msa0154320:three_prime_UTR
AAAATCTTTGGGGAGAATGT+GGG 0.532039 1_4:+18835127 Msa0154320:three_prime_UTR
ATTGTGTTACCGTATTATAC+CGG 0.533901 1_4:+18834947 Msa0154320:CDS
AAGAAGCTTTGGTTGTTGGA+GGG 0.534987 1_4:+18835029 Msa0154320:three_prime_UTR
TTTGCTTCATGTTCTCAAAG+AGG 0.537931 1_4:+18835294 Msa0154320:three_prime_UTR
ATTTCTTCTAGGTCCCATCC+AGG 0.541890 1_4:-18834461 None:intergenic
AACTAGTGAAGTACAAAGGT+TGG 0.543343 1_4:+18835053 Msa0154320:three_prime_UTR
TGTGTGTAGGAAAGAAGCTT+TGG 0.547839 1_4:+18835018 Msa0154320:three_prime_UTR
GCGTCGAAACCGGTATAATA+CGG 0.549726 1_4:-18834956 None:intergenic
TTTGATTGCAAAGTCACCAC+CGG 0.550429 1_4:+18835222 Msa0154320:three_prime_UTR
TTGTCACATACATAATGGTA+AGG 0.559614 1_4:-18834548 None:intergenic
AAATCTTTGGGGAGAATGTG+GGG 0.568787 1_4:+18835128 Msa0154320:three_prime_UTR
TTGGCAAATGGAAGAAACAA+TGG 0.577416 1_4:+18834511 Msa0154320:CDS
ATGTAGTCAAATTTGTGCAA+TGG 0.580987 1_4:+18835318 Msa0154320:three_prime_UTR
ACGAAGGTTACTTTGAGAAG+CGG 0.602692 1_4:-18835508 None:intergenic
CTCAACATTACTCTTTCCGG+TGG 0.610843 1_4:-18835238 None:intergenic
GATTATATGTAAAATCTTTG+GGG 0.628144 1_4:+18835117 Msa0154320:three_prime_UTR
AGTATGTAATATCAAAACGA+TGG 0.650160 1_4:+18835401 Msa0154320:three_prime_UTR
AATGTGCAACATGTGTGTGT+AGG 0.666637 1_4:+18835005 Msa0154320:three_prime_UTR
GGGAAACTAGTGAAGTACAA+AGG 0.675405 1_4:+18835049 Msa0154320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAAAAAAATAGATTTA+AGG + chr1_4:18835549-18835568 Msa0154320:three_prime_UTR 10.0%
!! TGAAAAAAAAAATAGATTTA+AGG + chr1_4:18835549-18835568 Msa0154320:three_prime_UTR 10.0%
!!! TACTTTTTTATTTATGCAAT+TGG - chr1_4:18835385-18835404 None:intergenic 15.0%
!!! TACTTTTTTATTTATGCAAT+TGG - chr1_4:18835385-18835404 None:intergenic 15.0%
!! ATGAATATTATCTCAAGAAA+AGG + chr1_4:18834662-18834681 Msa0154320:intron 20.0%
!!! AAAAGATTTTTTTTACCATC+TGG + chr1_4:18834707-18834726 Msa0154320:intron 20.0%
!! TATAATCTTAATCTTTGCAA+AGG + chr1_4:18834748-18834767 Msa0154320:intron 20.0%
!! ATAATCTTAATCTTTGCAAA+GGG + chr1_4:18834749-18834768 Msa0154320:intron 20.0%
!! AGGATTATATGTAAAATCTT+TGG + chr1_4:18835115-18835134 Msa0154320:three_prime_UTR 20.0%
!! GGATTATATGTAAAATCTTT+GGG + chr1_4:18835116-18835135 Msa0154320:three_prime_UTR 20.0%
!! GATTATATGTAAAATCTTTG+GGG + chr1_4:18835117-18835136 Msa0154320:three_prime_UTR 20.0%
!! ATGAATATTATCTCAAGAAA+AGG + chr1_4:18834662-18834681 Msa0154320:intron 20.0%
!!! AAAAGATTTTTTTTACCATC+TGG + chr1_4:18834707-18834726 Msa0154320:intron 20.0%
!! TATAATCTTAATCTTTGCAA+AGG + chr1_4:18834748-18834767 Msa0154320:intron 20.0%
!! ATAATCTTAATCTTTGCAAA+GGG + chr1_4:18834749-18834768 Msa0154320:intron 20.0%
!! AGGATTATATGTAAAATCTT+TGG + chr1_4:18835115-18835134 Msa0154320:three_prime_UTR 20.0%
!! GGATTATATGTAAAATCTTT+GGG + chr1_4:18835116-18835135 Msa0154320:three_prime_UTR 20.0%
!! GATTATATGTAAAATCTTTG+GGG + chr1_4:18835117-18835136 Msa0154320:three_prime_UTR 20.0%
! TAAGGCTATTCATCATTATA+TGG - chr1_4:18834412-18834431 None:intergenic 25.0%
!!! AAGTTTTGTCACATACATAA+TGG - chr1_4:18834556-18834575 None:intergenic 25.0%
! TGGAAAGATGATAATTTGAA+GGG - chr1_4:18834580-18834599 None:intergenic 25.0%
! ATGGAAAGATGATAATTTGA+AGG - chr1_4:18834581-18834600 None:intergenic 25.0%
! ATTATAAGTGGAAGAGATAT+AGG - chr1_4:18834734-18834753 None:intergenic 25.0%
! GCAAAGATTAAGATTATAAG+TGG - chr1_4:18834746-18834765 None:intergenic 25.0%
!! ATTTTCCCACTTTATTGTAT+TGG + chr1_4:18834788-18834807 Msa0154320:intron 25.0%
! ATAATCAGCTTCATTATCAA+AGG - chr1_4:18834852-18834871 None:intergenic 25.0%
!! TGATAATGAAGCTGATTATA+AGG + chr1_4:18834853-18834872 Msa0154320:intron 25.0%
! TTTGCATGCAAGTTTATATT+TGG + chr1_4:18834919-18834938 Msa0154320:CDS 25.0%
!! TTGTTAGTGTTCTTTGTTTA+AGG + chr1_4:18835095-18835114 Msa0154320:three_prime_UTR 25.0%
!!! TAGTTGGATTTTGATAAGTT+TGG + chr1_4:18835189-18835208 Msa0154320:three_prime_UTR 25.0%
! AGTATGTAATATCAAAACGA+TGG + chr1_4:18835401-18835420 Msa0154320:three_prime_UTR 25.0%
!! AAAACGATGGTTTAAAATTG+TGG + chr1_4:18835414-18835433 Msa0154320:three_prime_UTR 25.0%
! AATTGTGGTATGCAATATAA+AGG + chr1_4:18835429-18835448 Msa0154320:three_prime_UTR 25.0%
! ATTGTGGTATGCAATATAAA+GGG + chr1_4:18835430-18835449 Msa0154320:three_prime_UTR 25.0%
!!! ATCACAACTACAATTTTGAT+CGG + chr1_4:18835475-18835494 Msa0154320:three_prime_UTR 25.0%
! TAAGGCTATTCATCATTATA+TGG - chr1_4:18834412-18834431 None:intergenic 25.0%
!!! AAGTTTTGTCACATACATAA+TGG - chr1_4:18834556-18834575 None:intergenic 25.0%
! TGGAAAGATGATAATTTGAA+GGG - chr1_4:18834580-18834599 None:intergenic 25.0%
! ATGGAAAGATGATAATTTGA+AGG - chr1_4:18834581-18834600 None:intergenic 25.0%
! ATTATAAGTGGAAGAGATAT+AGG - chr1_4:18834734-18834753 None:intergenic 25.0%
! GCAAAGATTAAGATTATAAG+TGG - chr1_4:18834746-18834765 None:intergenic 25.0%
!! ATTTTCCCACTTTATTGTAT+TGG + chr1_4:18834788-18834807 Msa0154320:intron 25.0%
! ATAATCAGCTTCATTATCAA+AGG - chr1_4:18834852-18834871 None:intergenic 25.0%
!! TGATAATGAAGCTGATTATA+AGG + chr1_4:18834853-18834872 Msa0154320:intron 25.0%
! TTTGCATGCAAGTTTATATT+TGG + chr1_4:18834919-18834938 Msa0154320:CDS 25.0%
!! TTGTTAGTGTTCTTTGTTTA+AGG + chr1_4:18835095-18835114 Msa0154320:three_prime_UTR 25.0%
!!! TAGTTGGATTTTGATAAGTT+TGG + chr1_4:18835189-18835208 Msa0154320:three_prime_UTR 25.0%
! AGTATGTAATATCAAAACGA+TGG + chr1_4:18835401-18835420 Msa0154320:three_prime_UTR 25.0%
!! AAAACGATGGTTTAAAATTG+TGG + chr1_4:18835414-18835433 Msa0154320:three_prime_UTR 25.0%
! AATTGTGGTATGCAATATAA+AGG + chr1_4:18835429-18835448 Msa0154320:three_prime_UTR 25.0%
! ATTGTGGTATGCAATATAAA+GGG + chr1_4:18835430-18835449 Msa0154320:three_prime_UTR 25.0%
!!! ATCACAACTACAATTTTGAT+CGG + chr1_4:18835475-18835494 Msa0154320:three_prime_UTR 25.0%
!! TAATGATGAATAGCCTTATC+TGG + chr1_4:18834414-18834433 Msa0154320:exon 30.0%
ACTTATGCATTTGAAAAAGG+TGG - chr1_4:18834492-18834511 None:intergenic 30.0%
CCAACTTATGCATTTGAAAA+AGG - chr1_4:18834495-18834514 None:intergenic 30.0%
!!! CCTTTTTCAAATGCATAAGT+TGG + chr1_4:18834492-18834511 Msa0154320:CDS 30.0%
TTGTCACATACATAATGGTA+AGG - chr1_4:18834551-18834570 None:intergenic 30.0%
AAGAAATTGTTGTGAAGAGA+AGG - chr1_4:18834616-18834635 None:intergenic 30.0%
ATTGTGTTACCGTATTATAC+CGG + chr1_4:18834947-18834966 Msa0154320:CDS 30.0%
TTCCATCAACACTTTAATCA+TGG - chr1_4:18835164-18835183 None:intergenic 30.0%
!! TGATTAAAGTGTTGATGGAA+AGG + chr1_4:18835164-18835183 Msa0154320:three_prime_UTR 30.0%
ATGTAGTCAAATTTGTGCAA+TGG + chr1_4:18835318-18835337 Msa0154320:three_prime_UTR 30.0%
! ATGCAATATAAAGGGTTTTG+AGG + chr1_4:18835438-18835457 Msa0154320:three_prime_UTR 30.0%
!! AGACTTTAAAACAGCATCTT+AGG - chr1_4:18835614-18835633 None:intergenic 30.0%
!! TAATGATGAATAGCCTTATC+TGG + chr1_4:18834414-18834433 Msa0154320:exon 30.0%
ACTTATGCATTTGAAAAAGG+TGG - chr1_4:18834492-18834511 None:intergenic 30.0%
CCAACTTATGCATTTGAAAA+AGG - chr1_4:18834495-18834514 None:intergenic 30.0%
!!! CCTTTTTCAAATGCATAAGT+TGG + chr1_4:18834492-18834511 Msa0154320:CDS 30.0%
TTGTCACATACATAATGGTA+AGG - chr1_4:18834551-18834570 None:intergenic 30.0%
AAGAAATTGTTGTGAAGAGA+AGG - chr1_4:18834616-18834635 None:intergenic 30.0%
ATTGTGTTACCGTATTATAC+CGG + chr1_4:18834947-18834966 Msa0154320:CDS 30.0%
TTCCATCAACACTTTAATCA+TGG - chr1_4:18835164-18835183 None:intergenic 30.0%
!! TGATTAAAGTGTTGATGGAA+AGG + chr1_4:18835164-18835183 Msa0154320:three_prime_UTR 30.0%
ATGTAGTCAAATTTGTGCAA+TGG + chr1_4:18835318-18835337 Msa0154320:three_prime_UTR 30.0%
! ATGCAATATAAAGGGTTTTG+AGG + chr1_4:18835438-18835457 Msa0154320:three_prime_UTR 30.0%
!! AGACTTTAAAACAGCATCTT+AGG - chr1_4:18835614-18835633 None:intergenic 30.0%
!!! GTTGGAATGTTGGGTTTTTT+GGG - chr1_4:18834442-18834461 None:intergenic 35.0%
! CAAATGCATAAGTTGGCAAA+TGG + chr1_4:18834499-18834518 Msa0154320:CDS 35.0%
TTGGCAAATGGAAGAAACAA+TGG + chr1_4:18834511-18834530 Msa0154320:CDS 35.0%
GAGAAGGACTAAGATATCAA+TGG - chr1_4:18834600-18834619 None:intergenic 35.0%
CAGGACCAATACAATAAAGT+GGG - chr1_4:18834796-18834815 None:intergenic 35.0%
AGAACCAATAGAAGAATTGC+AGG - chr1_4:18834815-18834834 None:intergenic 35.0%
AACTAGTGAAGTACAAAGGT+TGG + chr1_4:18835053-18835072 Msa0154320:three_prime_UTR 35.0%
! TAAAATCTTTGGGGAGAATG+TGG + chr1_4:18835126-18835145 Msa0154320:three_prime_UTR 35.0%
! AAAATCTTTGGGGAGAATGT+GGG + chr1_4:18835127-18835146 Msa0154320:three_prime_UTR 35.0%
!! GTCCATGATTAAAGTGTTGA+TGG + chr1_4:18835159-18835178 Msa0154320:three_prime_UTR 35.0%
! TGTTGATGGAAAGGTTTAGT+TGG + chr1_4:18835173-18835192 Msa0154320:three_prime_UTR 35.0%
ACTCTCAACATTACTCTTTC+CGG - chr1_4:18835244-18835263 None:intergenic 35.0%
!!! ATGTTGAGAGTTGTTGCTTT+TGG + chr1_4:18835253-18835272 Msa0154320:three_prime_UTR 35.0%
TTTGCTTCATGTTCTCAAAG+AGG + chr1_4:18835294-18835313 Msa0154320:three_prime_UTR 35.0%
AATTTGTGCAATGGCTTATG+TGG + chr1_4:18835327-18835346 Msa0154320:three_prime_UTR 35.0%
TGTGGCAGAAAAATTGAGAT+TGG + chr1_4:18835345-18835364 Msa0154320:three_prime_UTR 35.0%
!! GTCGTTTTAAATTGTGACGA+AGG - chr1_4:18835527-18835546 None:intergenic 35.0%
!!! GTTGGAATGTTGGGTTTTTT+GGG - chr1_4:18834442-18834461 None:intergenic 35.0%
! CAAATGCATAAGTTGGCAAA+TGG + chr1_4:18834499-18834518 Msa0154320:CDS 35.0%
TTGGCAAATGGAAGAAACAA+TGG + chr1_4:18834511-18834530 Msa0154320:CDS 35.0%
GAGAAGGACTAAGATATCAA+TGG - chr1_4:18834600-18834619 None:intergenic 35.0%
CAGGACCAATACAATAAAGT+GGG - chr1_4:18834796-18834815 None:intergenic 35.0%
AGAACCAATAGAAGAATTGC+AGG - chr1_4:18834815-18834834 None:intergenic 35.0%
AACTAGTGAAGTACAAAGGT+TGG + chr1_4:18835053-18835072 Msa0154320:three_prime_UTR 35.0%
! TAAAATCTTTGGGGAGAATG+TGG + chr1_4:18835126-18835145 Msa0154320:three_prime_UTR 35.0%
! AAAATCTTTGGGGAGAATGT+GGG + chr1_4:18835127-18835146 Msa0154320:three_prime_UTR 35.0%
!! GTCCATGATTAAAGTGTTGA+TGG + chr1_4:18835159-18835178 Msa0154320:three_prime_UTR 35.0%
! TGTTGATGGAAAGGTTTAGT+TGG + chr1_4:18835173-18835192 Msa0154320:three_prime_UTR 35.0%
ACTCTCAACATTACTCTTTC+CGG - chr1_4:18835244-18835263 None:intergenic 35.0%
!!! ATGTTGAGAGTTGTTGCTTT+TGG + chr1_4:18835253-18835272 Msa0154320:three_prime_UTR 35.0%
TTTGCTTCATGTTCTCAAAG+AGG + chr1_4:18835294-18835313 Msa0154320:three_prime_UTR 35.0%
AATTTGTGCAATGGCTTATG+TGG + chr1_4:18835327-18835346 Msa0154320:three_prime_UTR 35.0%
TGTGGCAGAAAAATTGAGAT+TGG + chr1_4:18835345-18835364 Msa0154320:three_prime_UTR 35.0%
!! GTCGTTTTAAATTGTGACGA+AGG - chr1_4:18835527-18835546 None:intergenic 35.0%
!!! GGTTGGAATGTTGGGTTTTT+TGG - chr1_4:18834443-18834462 None:intergenic 40.0%
AAAAACCCAACATTCCAACC+TGG + chr1_4:18834443-18834462 Msa0154320:CDS 40.0%
AGGTGGCATGAATTTCTTCT+AGG - chr1_4:18834475-18834494 None:intergenic 40.0%
GCAGGACCAATACAATAAAG+TGG - chr1_4:18834797-18834816 None:intergenic 40.0%
TGGTCCTGCAATTCTTCTAT+TGG + chr1_4:18834808-18834827 Msa0154320:intron 40.0%
TTTGCAGCATCAACAGTTTC+TGG + chr1_4:18834893-18834912 Msa0154320:intron 40.0%
AATGTGCAACATGTGTGTGT+AGG + chr1_4:18835005-18835024 Msa0154320:three_prime_UTR 40.0%
TGTGTGTAGGAAAGAAGCTT+TGG + chr1_4:18835018-18835037 Msa0154320:three_prime_UTR 40.0%
! AGGAAAGAAGCTTTGGTTGT+TGG + chr1_4:18835025-18835044 Msa0154320:three_prime_UTR 40.0%
! AAAGAAGCTTTGGTTGTTGG+AGG + chr1_4:18835028-18835047 Msa0154320:three_prime_UTR 40.0%
! AAGAAGCTTTGGTTGTTGGA+GGG + chr1_4:18835029-18835048 Msa0154320:three_prime_UTR 40.0%
GGGAAACTAGTGAAGTACAA+AGG + chr1_4:18835049-18835068 Msa0154320:three_prime_UTR 40.0%
! AAATCTTTGGGGAGAATGTG+GGG + chr1_4:18835128-18835147 Msa0154320:three_prime_UTR 40.0%
TTTGATTGCAAAGTCACCAC+CGG + chr1_4:18835222-18835241 Msa0154320:three_prime_UTR 40.0%
!!! TTGAGAGTTGTTGCTTTTGG+AGG + chr1_4:18835256-18835275 Msa0154320:three_prime_UTR 40.0%
! ACGAAGGTTACTTTGAGAAG+CGG - chr1_4:18835511-18835530 None:intergenic 40.0%
!!! GGTTGGAATGTTGGGTTTTT+TGG - chr1_4:18834443-18834462 None:intergenic 40.0%
AAAAACCCAACATTCCAACC+TGG + chr1_4:18834443-18834462 Msa0154320:CDS 40.0%
AGGTGGCATGAATTTCTTCT+AGG - chr1_4:18834475-18834494 None:intergenic 40.0%
GCAGGACCAATACAATAAAG+TGG - chr1_4:18834797-18834816 None:intergenic 40.0%
TGGTCCTGCAATTCTTCTAT+TGG + chr1_4:18834808-18834827 Msa0154320:intron 40.0%
TTTGCAGCATCAACAGTTTC+TGG + chr1_4:18834893-18834912 Msa0154320:intron 40.0%
AATGTGCAACATGTGTGTGT+AGG + chr1_4:18835005-18835024 Msa0154320:three_prime_UTR 40.0%
TGTGTGTAGGAAAGAAGCTT+TGG + chr1_4:18835018-18835037 Msa0154320:three_prime_UTR 40.0%
! AGGAAAGAAGCTTTGGTTGT+TGG + chr1_4:18835025-18835044 Msa0154320:three_prime_UTR 40.0%
! AAAGAAGCTTTGGTTGTTGG+AGG + chr1_4:18835028-18835047 Msa0154320:three_prime_UTR 40.0%
! AAGAAGCTTTGGTTGTTGGA+GGG + chr1_4:18835029-18835048 Msa0154320:three_prime_UTR 40.0%
GGGAAACTAGTGAAGTACAA+AGG + chr1_4:18835049-18835068 Msa0154320:three_prime_UTR 40.0%
! AAATCTTTGGGGAGAATGTG+GGG + chr1_4:18835128-18835147 Msa0154320:three_prime_UTR 40.0%
TTTGATTGCAAAGTCACCAC+CGG + chr1_4:18835222-18835241 Msa0154320:three_prime_UTR 40.0%
!!! TTGAGAGTTGTTGCTTTTGG+AGG + chr1_4:18835256-18835275 Msa0154320:three_prime_UTR 40.0%
! ACGAAGGTTACTTTGAGAAG+CGG - chr1_4:18835511-18835530 None:intergenic 40.0%
!!! GGTTTTTTGGGAGCCAGATA+AGG - chr1_4:18834430-18834449 None:intergenic 45.0%
ATTTCTTCTAGGTCCCATCC+AGG - chr1_4:18834464-18834483 None:intergenic 45.0%
GGAAGAGATATAGGACCAGA+TGG - chr1_4:18834725-18834744 None:intergenic 45.0%
GCGTCGAAACCGGTATAATA+CGG - chr1_4:18834959-18834978 None:intergenic 45.0%
CTGATGTCTTCGACACTTTC+AGG + chr1_4:18834980-18834999 Msa0154320:exon 45.0%
CTCAACATTACTCTTTCCGG+TGG - chr1_4:18835241-18835260 None:intergenic 45.0%
!!! GGTTTTTTGGGAGCCAGATA+AGG - chr1_4:18834430-18834449 None:intergenic 45.0%
ATTTCTTCTAGGTCCCATCC+AGG - chr1_4:18834464-18834483 None:intergenic 45.0%
GGAAGAGATATAGGACCAGA+TGG - chr1_4:18834725-18834744 None:intergenic 45.0%
GCGTCGAAACCGGTATAATA+CGG - chr1_4:18834959-18834978 None:intergenic 45.0%
CTGATGTCTTCGACACTTTC+AGG + chr1_4:18834980-18834999 Msa0154320:exon 45.0%
CTCAACATTACTCTTTCCGG+TGG - chr1_4:18835241-18835260 None:intergenic 45.0%
ACCCAACATTCCAACCTGGA+TGG + chr1_4:18834447-18834466 Msa0154320:CDS 50.0%
! CCCATCCAGGTTGGAATGTT+GGG - chr1_4:18834451-18834470 None:intergenic 50.0%
CCCAACATTCCAACCTGGAT+GGG + chr1_4:18834448-18834467 Msa0154320:CDS 50.0%
! TCCCATCCAGGTTGGAATGT+TGG - chr1_4:18834452-18834471 None:intergenic 50.0%
AGACATCAGTGCGTCGAAAC+CGG - chr1_4:18834969-18834988 None:intergenic 50.0%
ACCCAACATTCCAACCTGGA+TGG + chr1_4:18834447-18834466 Msa0154320:CDS 50.0%
! CCCATCCAGGTTGGAATGTT+GGG - chr1_4:18834451-18834470 None:intergenic 50.0%
CCCAACATTCCAACCTGGAT+GGG + chr1_4:18834448-18834467 Msa0154320:CDS 50.0%
! TCCCATCCAGGTTGGAATGT+TGG - chr1_4:18834452-18834471 None:intergenic 50.0%
AGACATCAGTGCGTCGAAAC+CGG - chr1_4:18834969-18834988 None:intergenic 50.0%
CTTCTAGGTCCCATCCAGGT+TGG - chr1_4:18834460-18834479 None:intergenic 55.0%
CTTCTAGGTCCCATCCAGGT+TGG - chr1_4:18834460-18834479 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 18834391 18835698 18834391 ID=Msa0154320;Name=Msa0154320
chr1_4 mRNA 18834391 18835698 18834391 ID=Msa0154320-mRNA-1;Parent=Msa0154320;Name=Msa0154320-mRNA-1;_AED=0.13;_eAED=0.13;_QI=25|1|1|1|0|0|2|715|75
chr1_4 exon 18834391 18834559 18834391 ID=Msa0154320-mRNA-1:exon:6903;Parent=Msa0154320-mRNA-1
chr1_4 exon 18834900 18835698 18834900 ID=Msa0154320-mRNA-1:exon:6904;Parent=Msa0154320-mRNA-1
chr1_4 five_prime_UTR 18834391 18834415 18834391 ID=Msa0154320-mRNA-1:five_prime_utr;Parent=Msa0154320-mRNA-1
chr1_4 CDS 18834416 18834559 18834416 ID=Msa0154320-mRNA-1:cds;Parent=Msa0154320-mRNA-1
chr1_4 CDS 18834900 18834983 18834900 ID=Msa0154320-mRNA-1:cds;Parent=Msa0154320-mRNA-1
chr1_4 three_prime_UTR 18834984 18835698 18834984 ID=Msa0154320-mRNA-1:three_prime_utr;Parent=Msa0154320-mRNA-1
Gene Sequence

>Msa0154320

ATGATGAATAGCCTTATCTGGCTCCCAAAAAACCCAACATTCCAACCTGGATGGGACCTAGAAGAAATTCATGCCACCTTTTTCAAATGCATAAGTTGGCAAATGGAAGAAACAATGGATGCAATCAATTGTCCTTACCATTATCATCAACAGTTTCTGGAATTTTGCATGCAAGTTTATATTTGGATTCAATTGTGTTACCGTATTATACCGGTTTCGACGCACTGA
Protein sequence

>Msa0154320

MMNSLIWLPKNPTFQPGWDLEEIHATFFKCISWQMEETMDAINCPYHYHQQFLEFCMQVYIWIQLCYRIIPVSTH*