AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0070680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070680 MtrunA17_Chr1g0176101 99.451 364 2 0 1 364 1 364 0.0 756
Msa0070680 MtrunA17_Chr2g0309941 47.411 367 167 7 4 347 17 380 8.78e-113 333
Msa0070680 MtrunA17_Chr1g0207581 48.045 358 162 7 4 347 74 421 1.75e-112 335
Msa0070680 MtrunA17_Chr1g0152841 52.885 312 133 4 48 347 111 420 1.96e-111 333
Msa0070680 MtrunA17_Chr3g0134521 48.000 300 144 4 60 348 93 391 8.26e-100 301
Msa0070680 MtrunA17_Chr3g0112781 43.072 332 154 5 48 350 63 388 1.31e-90 279
Msa0070680 MtrunA17_Chr5g0436191 41.615 322 164 5 50 350 58 376 7.04e-85 263
Msa0070680 MtrunA17_Chr1g0154851 40.810 321 173 5 40 350 55 368 6.00e-75 236
Msa0070680 MtrunA17_Chr5g0403841 40.523 306 172 4 50 347 72 375 3.31e-71 227
Msa0070680 MtrunA17_Chr3g0141481 36.950 341 193 7 28 350 41 377 5.55e-70 224
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070680 AT5G06610.1 61.281 359 132 5 1 354 1 357 3.68e-157 446
Msa0070680 AT3G19540.1 49.544 329 146 5 37 347 108 434 6.51e-113 338
Msa0070680 AT1G27690.1 47.671 365 172 5 4 350 49 412 8.63e-112 333
Msa0070680 AT1G49840.1 46.685 362 170 8 4 347 86 442 1.89e-108 327
Msa0070680 AT1G79420.1 42.931 389 174 8 4 350 20 402 3.62e-100 303
Msa0070680 AT5G05840.1 43.558 326 157 5 48 349 66 388 3.90e-92 283
Msa0070680 AT5G66740.1 40.483 331 180 6 32 347 38 366 4.89e-74 234
Msa0070680 AT3G55720.1 39.339 333 165 7 48 347 62 390 1.30e-70 227
Msa0070680 AT1G75160.1 40.193 311 168 8 55 350 87 394 1.35e-66 216

Find 101 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGCTTCTTAGTGTTCT+TGG 0.194274 1_2:-41319753 Msa0070680:CDS
GAGTTCTAACCCACATATTC+TGG 0.196819 1_2:-41317189 Msa0070680:three_prime_UTR
GTTAATGGAACTTCAGAAAA+AGG 0.220805 1_2:-41318812 Msa0070680:CDS
ACCTCATTGAGGGGTGATTT+AGG 0.222575 1_2:+41319702 None:intergenic
TCTGCATGGCTTGAGGGATT+AGG 0.236892 1_2:-41317297 Msa0070680:three_prime_UTR
CTTTGTTTCAAGCTTAGATA+TGG 0.243678 1_2:-41319928 Msa0070680:exon
TTGATAGGTTCTTCTGATAA+TGG 0.264140 1_2:+41319894 None:intergenic
GCATACATTGATTGTTAGTA+TGG 0.264720 1_2:-41317149 Msa0070680:three_prime_UTR
TCCTCTTCGCCGTGCACTTC+AGG 0.275448 1_2:-41318642 Msa0070680:intron
ATGATAAAGCATGCTATCTT+TGG 0.286269 1_2:-41317807 Msa0070680:CDS
CTGCATGGCTTGAGGGATTA+GGG 0.292410 1_2:-41317296 Msa0070680:three_prime_UTR
ATCTCTATCTGCCAATTATC+AGG 0.310067 1_2:+41318764 None:intergenic
GTCGGTGGTGGTGGTGTTAA+TGG 0.312645 1_2:-41318827 Msa0070680:CDS
TAGGGACAAATCCTACATAT+TGG 0.324957 1_2:-41317716 Msa0070680:CDS
GAAACAAAGGAAAATGAAAA+TGG 0.359062 1_2:+41319942 None:intergenic
GGCAAATGGTTCCTGATAAT+TGG 0.359506 1_2:-41318775 Msa0070680:CDS
TTGACAAGGATTCAAGCAAT+AGG 0.367414 1_2:-41317735 Msa0070680:CDS
AGGGGTGATTTAGGATGAAT+AGG 0.373094 1_2:+41319711 None:intergenic
CTCGGTGTGCACGCTGCTAA+AGG 0.374472 1_2:-41318674 Msa0070680:CDS
TCGTGTTCTGCATGGCTTGA+GGG 0.383980 1_2:-41317303 Msa0070680:three_prime_UTR
GTCCACAACAACATCTTGAT+TGG 0.384230 1_2:-41317467 Msa0070680:CDS
CAACAATTCACAGCTGCAAC+AGG 0.388041 1_2:-41318932 Msa0070680:CDS
TACTACCAGCAAGAACCTTT+TGG 0.394205 1_2:+41318729 None:intergenic
AGGGACAAATCCTACATATT+GGG 0.397990 1_2:-41317715 Msa0070680:CDS
TGACAAGGATTCAAGCAATA+GGG 0.405287 1_2:-41317734 Msa0070680:CDS
TGTTGTCGCTTCGTTCGATT+AGG 0.414182 1_2:+41317838 None:intergenic
TCAAAATATAACTCTAAGAA+AGG 0.430604 1_2:-41319849 Msa0070680:CDS
ATTGTTGTTTCCCAATATGT+AGG 0.441158 1_2:+41317705 None:intergenic
GGTGGCCAAAAGGTTCTTGC+TGG 0.442056 1_2:-41318734 Msa0070680:CDS
AAGGTTTGGTGATAATCTTA+AGG 0.444872 1_2:-41317604 Msa0070680:CDS
TACTTAACATGTATAATGAA+TGG 0.446313 1_2:-41317229 Msa0070680:three_prime_UTR
CTTGGATCGGTCGGTGGTGG+TGG 0.446484 1_2:-41318836 Msa0070680:CDS
GTGAAGAATGTGTTTGCAAC+CGG 0.450494 1_2:-41318887 Msa0070680:CDS
AGGATGGACCTGCTATTACT+AGG 0.461843 1_2:-41317584 Msa0070680:CDS
GCCATGCAGAACACGAGAAT+AGG 0.464980 1_2:+41317310 None:intergenic
AGTTATGGATGAATTGGAGT+TGG 0.469771 1_2:-41319821 Msa0070680:CDS
AAAACTCTCAGCCGATCAAA+AGG 0.469817 1_2:-41317862 Msa0070680:CDS
AGTTCTAACCCACATATTCT+GGG 0.472172 1_2:-41317188 Msa0070680:three_prime_UTR
TGATTGTTTCTTACCACCTC+AGG 0.473945 1_2:-41317505 Msa0070680:CDS
TCTCCTACACCCAGAATATG+TGG 0.477522 1_2:+41317179 None:intergenic
GATTGTTGTTGGTGGCCAAA+AGG 0.485888 1_2:-41318744 Msa0070680:CDS
TTTGTTTCAAGCTTAGATAT+GGG 0.486594 1_2:-41319927 Msa0070680:exon
GCCTATTCTCGTGTTCTGCA+TGG 0.489588 1_2:-41317311 Msa0070680:three_prime_UTR
CAAAGGAAAATGAAAATGGT+TGG 0.490005 1_2:+41319946 None:intergenic
TGGCGTCACACACCTTGGCT+CGG 0.492783 1_2:-41318692 Msa0070680:CDS
GAGCTTGGATCGGTCGGTGG+TGG 0.494261 1_2:-41318839 Msa0070680:CDS
ATGGTTGTCGTGGACGAGCT+TGG 0.495724 1_2:-41318854 Msa0070680:CDS
GATTTAGGATGAATAGGAAC+AGG 0.498652 1_2:+41319717 None:intergenic
ATACAGCTGATACTGCTAAA+GGG 0.506311 1_2:+41317943 None:intergenic
GAAGACTATAGAGATGTTGA+TGG 0.506980 1_2:-41317669 Msa0070680:CDS
TGCAACAGGGTGTAGGAAGT+TGG 0.515412 1_2:-41318918 Msa0070680:CDS
GATGTTGCATTTAATGTTCA+AGG 0.515683 1_2:-41317537 Msa0070680:CDS
GTAGTAACGGCACTATTGCT+TGG 0.521284 1_2:-41318712 Msa0070680:CDS
AAAAGAACTTACCTCATTGA+GGG 0.521839 1_2:+41319692 None:intergenic
TTTGGTGATAATCTTAAGGA+TGG 0.522450 1_2:-41317600 Msa0070680:CDS
AATACAGCTGATACTGCTAA+AGG 0.523894 1_2:+41317942 None:intergenic
TGGCAGATAGAGATTGTTGT+TGG 0.528000 1_2:-41318755 Msa0070680:CDS
TCTGTTACAGGTTTCGTCAT+CGG 0.528049 1_2:-41318969 Msa0070680:intron
GGTGTTATGATTGCACATGC+TGG 0.531989 1_2:-41317648 Msa0070680:CDS
CTCGTGTTCTGCATGGCTTG+AGG 0.532968 1_2:-41317304 Msa0070680:three_prime_UTR
GATAGCATGCTTTATCATCT+CGG 0.533661 1_2:+41317812 None:intergenic
CAGATAGAGATTGTTGTTGG+TGG 0.537302 1_2:-41318752 Msa0070680:CDS
TTTCAAGCTTAGATATGGGT+AGG 0.539926 1_2:-41319923 Msa0070680:exon
CATATCTAAGCTTGAAACAA+AGG 0.540284 1_2:+41319929 None:intergenic
TGGACCTGCTATTACTAGGT+TGG 0.544281 1_2:-41317580 Msa0070680:CDS
AAGGTTCTTGCTGGTAGTAA+CGG 0.544977 1_2:-41318725 Msa0070680:CDS
ATTCAGTGCAGCACAGCACA+TGG 0.562606 1_2:-41317922 Msa0070680:CDS
ACTGTGATGATAACAAGGTT+TGG 0.564007 1_2:-41317618 Msa0070680:CDS
TTGCTTGGCGTCACACACCT+TGG 0.565443 1_2:-41318697 Msa0070680:CDS
TCTTCCAACCTAGTAATAGC+AGG 0.565502 1_2:+41317576 None:intergenic
TTACTAGGTTGGAAGAGTCA+TGG 0.572091 1_2:-41317569 Msa0070680:CDS
CAATCTTTGTTAAGTTCCTG+AGG 0.573721 1_2:+41317489 None:intergenic
TGTCGTGGACGAGCTTGGAT+CGG 0.576957 1_2:-41318849 Msa0070680:CDS
GCAGCGTGCACACCGAGCCA+AGG 0.577284 1_2:+41318680 None:intergenic
GTGGACGAGCTTGGATCGGT+CGG 0.581244 1_2:-41318845 Msa0070680:CDS
ACCTGAAGTGCACGGCGAAG+AGG 0.581794 1_2:+41318641 None:intergenic
AACAGGGTGTAGGAAGTTGG+AGG 0.584583 1_2:-41318915 Msa0070680:CDS
CAACAACTGTGATGATAACA+AGG 0.586246 1_2:-41317623 Msa0070680:CDS
TTCAGTGCAGCACAGCACAT+GGG 0.587648 1_2:-41317921 Msa0070680:CDS
ATGTTGCATTTAATGTTCAA+GGG 0.590841 1_2:-41317536 Msa0070680:CDS
CAGAACAGAACACACTTCAA+AGG 0.599126 1_2:+41319975 None:intergenic
TCACAGCTGCAACAGGGTGT+AGG 0.606606 1_2:-41318925 Msa0070680:CDS
AAAAGTGACAATGGTTGTCG+TGG 0.609256 1_2:-41318864 Msa0070680:CDS
CTCCTACACCCAGAATATGT+GGG 0.613366 1_2:+41317180 None:intergenic
GACGAGCTTGGATCGGTCGG+TGG 0.615008 1_2:-41318842 Msa0070680:CDS
TAGAGGACTCTTATTTGACA+AGG 0.615017 1_2:-41317749 Msa0070680:CDS
TCTTTGTTAAGTTCCTGAGG+TGG 0.615936 1_2:+41317492 None:intergenic
TCAGTGCAGCACAGCACATG+GGG 0.622785 1_2:-41317920 Msa0070680:CDS
AACCCACATATTCTGGGTGT+AGG 0.626622 1_2:-41317182 Msa0070680:three_prime_UTR
TCCTAAATCACCCCTCAATG+AGG 0.627312 1_2:-41319703 Msa0070680:intron
CTCCAATCAAGATGTTGTTG+TGG 0.628883 1_2:+41317465 None:intergenic
GAACACTAAGAAGCATCTTG+AGG 0.640003 1_2:+41319757 None:intergenic
ACAGGGTGTAGGAAGTTGGA+GGG 0.647737 1_2:-41318914 Msa0070680:CDS
AACAATTCACAGCTGCAACA+GGG 0.653620 1_2:-41318931 Msa0070680:CDS
AAAGAACTTACCTCATTGAG+GGG 0.670876 1_2:+41319693 None:intergenic
AATCGAACGAAGCGACAACA+CGG 0.675120 1_2:-41317835 Msa0070680:CDS
ATGAACTTACCTGAAGTGCA+CGG 0.677574 1_2:+41318633 None:intergenic
GGTGTGCACGCTGCTAAAGG+CGG 0.678174 1_2:-41318671 Msa0070680:CDS
TGCAACCGGAAAAGTGACAA+TGG 0.685929 1_2:-41318873 Msa0070680:CDS
CAGGGTGTAGGAAGTTGGAG+GGG 0.733318 1_2:-41318913 Msa0070680:CDS
CAGTGCAGCACAGCACATGG+GGG 0.743406 1_2:-41317919 Msa0070680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACATTAATATTAAAAG+GGG + chr1_2:41318076-41318095 None:intergenic 10.0%
!! TCATATATAAAAATAATTCA+TGG - chr1_2:41319715-41319734 Msa0070680:CDS 10.0%
!!! TTTTTTTAAACTTTTTAACT+AGG - chr1_2:41319743-41319762 Msa0070680:CDS 10.0%
!!! TAGTGTTATTTTATATAAAT+CGG + chr1_2:41319880-41319899 None:intergenic 10.0%
!! CATAAAAATAAAAATTTGCA+TGG + chr1_2:41317507-41317526 None:intergenic 15.0%
!! ATTTAGCAATATACTAAAAA+TGG + chr1_2:41317679-41317698 None:intergenic 15.0%
!!! ATATTGCTAAATATTTTTGT+CGG - chr1_2:41317687-41317706 Msa0070680:CDS 15.0%
!! TAGTTGAATGAAATTTATTA+GGG + chr1_2:41318992-41319011 None:intergenic 15.0%
!! ATAGTTGAATGAAATTTATT+AGG + chr1_2:41318993-41319012 None:intergenic 15.0%
!!! ACAATTAATGTTTGTTTTTA+AGG + chr1_2:41319148-41319167 None:intergenic 15.0%
!!! AGTGTTATTTTATATAAATC+GGG + chr1_2:41319879-41319898 None:intergenic 15.0%
!!! TCAAAATATAACTCTAAGAA+AGG - chr1_2:41317285-41317304 Msa0070680:three_prime_UTR 20.0%
!! TAAAACATGAGTTCAAATTT+GGG + chr1_2:41317761-41317780 None:intergenic 20.0%
!! TTAAAACATGAGTTCAAATT+TGG + chr1_2:41317762-41317781 None:intergenic 20.0%
!!! TTTTTTCTTTACTTTTGGTT+TGG + chr1_2:41317794-41317813 None:intergenic 20.0%
!!! AAGCATTTTTTCTTTACTTT+TGG + chr1_2:41317799-41317818 None:intergenic 20.0%
!! GAAAAAATGCTTCTAATTAT+TGG - chr1_2:41317807-41317826 Msa0070680:CDS 20.0%
!! AAAATAAAGATCACTGAAAA+AGG + chr1_2:41318138-41318157 None:intergenic 20.0%
!!! ATTTTGAATGTGATTGATTT+TGG - chr1_2:41318536-41318555 Msa0070680:intron 20.0%
!! TTAGAAGTTTATCAAATCAT+AGG + chr1_2:41319081-41319100 None:intergenic 20.0%
!!! TAATTGTAGTTTTGATATGT+AGG - chr1_2:41319161-41319180 Msa0070680:intron 20.0%
!!! AATTGTAGTTTTGATATGTA+GGG - chr1_2:41319162-41319181 Msa0070680:intron 20.0%
!!! TTAAACTTTTTAACTAGGTT+TGG - chr1_2:41319748-41319767 Msa0070680:CDS 20.0%
!! TACTTAACATGTATAATGAA+TGG - chr1_2:41319905-41319924 Msa0070680:CDS 20.0%
! GAAACAAAGGAAAATGAAAA+TGG + chr1_2:41317195-41317214 None:intergenic 25.0%
! TTTGTTTCAAGCTTAGATAT+GGG - chr1_2:41317207-41317226 Msa0070680:three_prime_UTR 25.0%
!! CTTTCATTTTCTTCATTGAT+AGG + chr1_2:41317258-41317277 None:intergenic 25.0%
!! TGTTCTTTGTTGATTGAAAA+TGG - chr1_2:41317956-41317975 Msa0070680:intron 25.0%
!!! ACTTTTAGCTAACTTTTTCA+AGG + chr1_2:41317992-41318011 None:intergenic 25.0%
!!! AGCTAAAAGTGTTCAAATTT+AGG - chr1_2:41318002-41318021 Msa0070680:intron 25.0%
! TTACATATACAAGGTAACAT+TGG + chr1_2:41318107-41318126 None:intergenic 25.0%
! GATCAAACTTTACATATACA+AGG + chr1_2:41318116-41318135 None:intergenic 25.0%
!! ATTTTGTTACAATCTGTTAC+AGG - chr1_2:41318153-41318172 Msa0070680:intron 25.0%
! TTAATGGAACTTCAGAAAAA+GGG - chr1_2:41318323-41318342 Msa0070680:intron 25.0%
! TCACATTCAAAATCAAGTTT+AGG + chr1_2:41318529-41318548 None:intergenic 25.0%
!!! AGAATTGATTTTGTGTCTAT+AGG + chr1_2:41318686-41318705 None:intergenic 25.0%
! TATAGACACAAAATCAATTC+TGG - chr1_2:41318685-41318704 Msa0070680:CDS 25.0%
! CTAAATATAAAGCATGTGTT+TGG + chr1_2:41318722-41318741 None:intergenic 25.0%
! ACACATGCTTTATATTTAGT+TGG - chr1_2:41318723-41318742 Msa0070680:CDS 25.0%
! GATAATGTGTTATGAACATA+TGG - chr1_2:41318768-41318787 Msa0070680:CDS 25.0%
! AGAAAAAGACAAGAAAGTTT+AGG + chr1_2:41319032-41319051 None:intergenic 25.0%
! ATGTTGCATTTAATGTTCAA+GGG - chr1_2:41319598-41319617 Msa0070680:intron 25.0%
CAAAGGAAAATGAAAATGGT+TGG + chr1_2:41317191-41317210 None:intergenic 30.0%
CATATCTAAGCTTGAAACAA+AGG + chr1_2:41317208-41317227 None:intergenic 30.0%
! CTTTGTTTCAAGCTTAGATA+TGG - chr1_2:41317206-41317225 Msa0070680:three_prime_UTR 30.0%
TTGATAGGTTCTTCTGATAA+TGG + chr1_2:41317243-41317262 None:intergenic 30.0%
! CTAAGAAAGGTTTTCAGTTA+TGG - chr1_2:41317298-41317317 Msa0070680:three_prime_UTR 30.0%
! GTTTTCAGTTATGGATGAAT+TGG - chr1_2:41317307-41317326 Msa0070680:three_prime_UTR 30.0%
AAAAGAACTTACCTCATTGA+GGG + chr1_2:41317445-41317464 None:intergenic 30.0%
AAAAAGAACTTACCTCATTG+AGG + chr1_2:41317446-41317465 None:intergenic 30.0%
!!! TTTACTTTTGGTTTGGTTGA+AGG + chr1_2:41317787-41317806 None:intergenic 30.0%
GTTAATGGAACTTCAGAAAA+AGG - chr1_2:41318322-41318341 Msa0070680:intron 30.0%
! TATGAACATATGGTGATGTT+TGG - chr1_2:41318778-41318797 Msa0070680:CDS 30.0%
! ATGTCATTTAACTACTGCTA+TGG - chr1_2:41318891-41318910 Msa0070680:CDS 30.0%
AAGCAAATGATCCAATTCAT+TGG + chr1_2:41318969-41318988 None:intergenic 30.0%
ATGATAAAGCATGCTATCTT+TGG - chr1_2:41319327-41319346 Msa0070680:intron 30.0%
!!! GGTTATTTTAGTCAAAGAAG+TGG - chr1_2:41319348-41319367 Msa0070680:intron 30.0%
!! ATTGTTGTTTCCCAATATGT+AGG + chr1_2:41319432-41319451 None:intergenic 30.0%
!! AAGGTTTGGTGATAATCTTA+AGG - chr1_2:41319530-41319549 Msa0070680:intron 30.0%
! TTTGGTGATAATCTTAAGGA+TGG - chr1_2:41319534-41319553 Msa0070680:intron 30.0%
GATGTTGCATTTAATGTTCA+AGG - chr1_2:41319597-41319616 Msa0070680:intron 30.0%
! GCATACATTGATTGTTAGTA+TGG - chr1_2:41319985-41320004 Msa0070680:five_prime_UTR 30.0%
TTTCAAGCTTAGATATGGGT+AGG - chr1_2:41317211-41317230 Msa0070680:three_prime_UTR 35.0%
AGTTATGGATGAATTGGAGT+TGG - chr1_2:41317313-41317332 Msa0070680:three_prime_UTR 35.0%
AAGATGCTTCTTAGTGTTCT+TGG - chr1_2:41317381-41317400 Msa0070680:three_prime_UTR 35.0%
TGAATAGGAACAGGAAAAAG+AGG + chr1_2:41317411-41317430 None:intergenic 35.0%
! GATTTAGGATGAATAGGAAC+AGG + chr1_2:41317420-41317439 None:intergenic 35.0%
AAAGAACTTACCTCATTGAG+GGG + chr1_2:41317444-41317463 None:intergenic 35.0%
AACATGAGTTCAAATTTGGG+TGG + chr1_2:41317758-41317777 None:intergenic 35.0%
GGATGGAATCAAATACTAGA+CGG - chr1_2:41317882-41317901 Msa0070680:CDS 35.0%
CAAATACTAGACGGTTTGAT+TGG - chr1_2:41317891-41317910 Msa0070680:CDS 35.0%
!!! AAAAAGGGTGTTTTGTGATG+TGG - chr1_2:41318338-41318357 Msa0070680:intron 35.0%
ATCTCTATCTGCCAATTATC+AGG + chr1_2:41318373-41318392 None:intergenic 35.0%
GACAAGAAAGTTTAGGAATG+TGG + chr1_2:41319025-41319044 None:intergenic 35.0%
ATACAGCTGATACTGCTAAA+GGG + chr1_2:41319194-41319213 None:intergenic 35.0%
AATACAGCTGATACTGCTAA+AGG + chr1_2:41319195-41319214 None:intergenic 35.0%
! TTCGATTAGGTCCTTTTGAT+CGG + chr1_2:41319286-41319305 None:intergenic 35.0%
GATAGCATGCTTTATCATCT+CGG + chr1_2:41319325-41319344 None:intergenic 35.0%
! TAGAGGACTCTTATTTGACA+AGG - chr1_2:41319385-41319404 Msa0070680:intron 35.0%
TTGACAAGGATTCAAGCAAT+AGG - chr1_2:41319399-41319418 Msa0070680:intron 35.0%
TGACAAGGATTCAAGCAATA+GGG - chr1_2:41319400-41319419 Msa0070680:intron 35.0%
TAGGGACAAATCCTACATAT+TGG - chr1_2:41319418-41319437 Msa0070680:intron 35.0%
AGGGACAAATCCTACATATT+GGG - chr1_2:41319419-41319438 Msa0070680:intron 35.0%
GAAGACTATAGAGATGTTGA+TGG - chr1_2:41319465-41319484 Msa0070680:intron 35.0%
CAACAACTGTGATGATAACA+AGG - chr1_2:41319511-41319530 Msa0070680:intron 35.0%
ACTGTGATGATAACAAGGTT+TGG - chr1_2:41319516-41319535 Msa0070680:intron 35.0%
CAATCTTTGTTAAGTTCCTG+AGG + chr1_2:41319648-41319667 None:intergenic 35.0%
! AGTTCTAACCCACATATTCT+GGG - chr1_2:41319946-41319965 Msa0070680:five_prime_UTR 35.0%
CAGAACAGAACACACTTCAA+AGG + chr1_2:41317162-41317181 None:intergenic 40.0%
!! GAACACTAAGAAGCATCTTG+AGG + chr1_2:41317380-41317399 None:intergenic 40.0%
! AGGGGTGATTTAGGATGAAT+AGG + chr1_2:41317426-41317445 None:intergenic 40.0%
TCTGTTACAGGTTTCGTCAT+CGG - chr1_2:41318165-41318184 Msa0070680:intron 40.0%
AACAATTCACAGCTGCAACA+GGG - chr1_2:41318203-41318222 Msa0070680:intron 40.0%
GTGAAGAATGTGTTTGCAAC+CGG - chr1_2:41318247-41318266 Msa0070680:intron 40.0%
!!! GACAACCATTGTCACTTTTC+CGG + chr1_2:41318269-41318288 None:intergenic 40.0%
AAAAGTGACAATGGTTGTCG+TGG - chr1_2:41318270-41318289 Msa0070680:intron 40.0%
! GTGTTTTGTGATGTGGCAAA+TGG - chr1_2:41318345-41318364 Msa0070680:intron 40.0%
GGCAAATGGTTCCTGATAAT+TGG - chr1_2:41318359-41318378 Msa0070680:intron 40.0%
TGGCAGATAGAGATTGTTGT+TGG - chr1_2:41318379-41318398 Msa0070680:intron 40.0%
! CAGATAGAGATTGTTGTTGG+TGG - chr1_2:41318382-41318401 Msa0070680:intron 40.0%
! TACTACCAGCAAGAACCTTT+TGG + chr1_2:41318408-41318427 None:intergenic 40.0%
AAGGTTCTTGCTGGTAGTAA+CGG - chr1_2:41318409-41318428 Msa0070680:intron 40.0%
ATGAACTTACCTGAAGTGCA+CGG + chr1_2:41318504-41318523 None:intergenic 40.0%
AAACTTCAGCACTTCAACAC+AGG - chr1_2:41318926-41318945 Msa0070680:CDS 40.0%
ATGGGGGAGAAACAAATCAA+CGG - chr1_2:41319231-41319250 Msa0070680:intron 40.0%
AAAACTCTCAGCCGATCAAA+AGG - chr1_2:41319272-41319291 Msa0070680:intron 40.0%
! TGGACTTTTAGTGCACTTAG+AGG - chr1_2:41319368-41319387 Msa0070680:intron 40.0%
TCTTCCAACCTAGTAATAGC+AGG + chr1_2:41319561-41319580 None:intergenic 40.0%
! TTACTAGGTTGGAAGAGTCA+TGG - chr1_2:41319565-41319584 Msa0070680:intron 40.0%
! TGATTGTTTCTTACCACCTC+AGG - chr1_2:41319629-41319648 Msa0070680:intron 40.0%
TCTTTGTTAAGTTCCTGAGG+TGG + chr1_2:41319645-41319664 None:intergenic 40.0%
GTCCACAACAACATCTTGAT+TGG - chr1_2:41319667-41319686 Msa0070680:intron 40.0%
CTCCAATCAAGATGTTGTTG+TGG + chr1_2:41319672-41319691 None:intergenic 40.0%
! GAGTTCTAACCCACATATTC+TGG - chr1_2:41319945-41319964 Msa0070680:five_prime_UTR 40.0%
TCCTAAATCACCCCTCAATG+AGG - chr1_2:41317431-41317450 Msa0070680:exon 45.0%
ACCTCATTGAGGGGTGATTT+AGG + chr1_2:41317435-41317454 None:intergenic 45.0%
ATCCGAAGATTTCCTCAGGA+TGG + chr1_2:41317866-41317885 None:intergenic 45.0%
ATCCTGAGGAAATCTTCGGA+TGG - chr1_2:41317865-41317884 Msa0070680:CDS 45.0%
TTCCATCCGAAGATTTCCTC+AGG + chr1_2:41317870-41317889 None:intergenic 45.0%
CAACAATTCACAGCTGCAAC+AGG - chr1_2:41318202-41318221 Msa0070680:intron 45.0%
TGCAACCGGAAAAGTGACAA+TGG - chr1_2:41318261-41318280 Msa0070680:intron 45.0%
!! GATTGTTGTTGGTGGCCAAA+AGG - chr1_2:41318390-41318409 Msa0070680:intron 45.0%
GTAGTAACGGCACTATTGCT+TGG - chr1_2:41318422-41318441 Msa0070680:intron 45.0%
! TGTTGTCGCTTCGTTCGATT+AGG + chr1_2:41319299-41319318 None:intergenic 45.0%
AATCGAACGAAGCGACAACA+CGG - chr1_2:41319299-41319318 Msa0070680:intron 45.0%
! GGTGTTATGATTGCACATGC+TGG - chr1_2:41319486-41319505 Msa0070680:intron 45.0%
AGGATGGACCTGCTATTACT+AGG - chr1_2:41319550-41319569 Msa0070680:intron 45.0%
TGGACCTGCTATTACTAGGT+TGG - chr1_2:41319554-41319573 Msa0070680:intron 45.0%
AACCCACATATTCTGGGTGT+AGG - chr1_2:41319952-41319971 Msa0070680:five_prime_UTR 45.0%
CTCCTACACCCAGAATATGT+GGG + chr1_2:41319957-41319976 None:intergenic 45.0%
TCTCCTACACCCAGAATATG+TGG + chr1_2:41319958-41319977 None:intergenic 45.0%
!! TATAAACATTAATATTAAAA+GGG + chr1_2:41318077-41318096 None:intergenic 5.0%
!! TTATAAACATTAATATTAAA+AGG + chr1_2:41318078-41318097 None:intergenic 5.0%
!! TTTAATATTAATGTTTATAA+TGG - chr1_2:41318078-41318097 Msa0070680:intron 5.0%
!! AGCCTGAGGGCGAGTTTTAT+CGG + chr1_2:41317839-41317858 None:intergenic 50.0%
GAAGATTTCCTCAGGATGGC+CGG + chr1_2:41317862-41317881 None:intergenic 50.0%
GGCCATCCTGAGGAAATCTT+CGG - chr1_2:41317861-41317880 Msa0070680:CDS 50.0%
TGCAACAGGGTGTAGGAAGT+TGG - chr1_2:41318216-41318235 Msa0070680:intron 50.0%
AACAGGGTGTAGGAAGTTGG+AGG - chr1_2:41318219-41318238 Msa0070680:intron 50.0%
ACAGGGTGTAGGAAGTTGGA+GGG - chr1_2:41318220-41318239 Msa0070680:intron 50.0%
CTAGCTGCAGGCCAATGAAT+TGG - chr1_2:41318955-41318974 Msa0070680:CDS 50.0%
ATTCAGTGCAGCACAGCACA+TGG - chr1_2:41319212-41319231 Msa0070680:intron 50.0%
TTCAGTGCAGCACAGCACAT+GGG - chr1_2:41319213-41319232 Msa0070680:intron 50.0%
GCCTATTCTCGTGTTCTGCA+TGG - chr1_2:41319823-41319842 Msa0070680:CDS 50.0%
GCCATGCAGAACACGAGAAT+AGG + chr1_2:41319827-41319846 None:intergenic 50.0%
TCGTGTTCTGCATGGCTTGA+GGG - chr1_2:41319831-41319850 Msa0070680:CDS 50.0%
TCTGCATGGCTTGAGGGATT+AGG - chr1_2:41319837-41319856 Msa0070680:CDS 50.0%
CTGCATGGCTTGAGGGATTA+GGG - chr1_2:41319838-41319857 Msa0070680:CDS 50.0%
ACCCGATAAAACTCGCCCTC+AGG - chr1_2:41317834-41317853 Msa0070680:CDS 55.0%
!! GCCTGAGGGCGAGTTTTATC+GGG + chr1_2:41317838-41317857 None:intergenic 55.0%
TAAAACTCGCCCTCAGGCTC+CGG - chr1_2:41317840-41317859 Msa0070680:CDS 55.0%
TCACAGCTGCAACAGGGTGT+AGG - chr1_2:41318209-41318228 Msa0070680:intron 55.0%
CAGGGTGTAGGAAGTTGGAG+GGG - chr1_2:41318221-41318240 Msa0070680:intron 55.0%
ATGGTTGTCGTGGACGAGCT+TGG - chr1_2:41318280-41318299 Msa0070680:intron 55.0%
TGTCGTGGACGAGCTTGGAT+CGG - chr1_2:41318285-41318304 Msa0070680:intron 55.0%
!! GTCGGTGGTGGTGGTGTTAA+TGG - chr1_2:41318307-41318326 Msa0070680:intron 55.0%
GGTGGCCAAAAGGTTCTTGC+TGG - chr1_2:41318400-41318419 Msa0070680:intron 55.0%
TTGCTTGGCGTCACACACCT+TGG - chr1_2:41318437-41318456 Msa0070680:intron 55.0%
CACAGGCATATGCTAGCTGC+AGG - chr1_2:41318943-41318962 Msa0070680:CDS 55.0%
TCAGTGCAGCACAGCACATG+GGG - chr1_2:41319214-41319233 Msa0070680:intron 55.0%
CTCGTGTTCTGCATGGCTTG+AGG - chr1_2:41319830-41319849 Msa0070680:CDS 55.0%
GTGGACGAGCTTGGATCGGT+CGG - chr1_2:41318289-41318308 Msa0070680:intron 60.0%
TGGCGTCACACACCTTGGCT+CGG - chr1_2:41318442-41318461 Msa0070680:intron 60.0%
! CTCGGTGTGCACGCTGCTAA+AGG - chr1_2:41318460-41318479 Msa0070680:intron 60.0%
GGTGTGCACGCTGCTAAAGG+CGG - chr1_2:41318463-41318482 Msa0070680:intron 60.0%
TCCTCTTCGCCGTGCACTTC+AGG - chr1_2:41318492-41318511 Msa0070680:intron 60.0%
ACCTGAAGTGCACGGCGAAG+AGG + chr1_2:41318496-41318515 None:intergenic 60.0%
CAGTGCAGCACAGCACATGG+GGG - chr1_2:41319215-41319234 Msa0070680:intron 60.0%
TCAGGATGGCCGGAGCCTGA+GGG + chr1_2:41317852-41317871 None:intergenic 65.0%
GACGAGCTTGGATCGGTCGG+TGG - chr1_2:41318292-41318311 Msa0070680:intron 65.0%
GAGCTTGGATCGGTCGGTGG+TGG - chr1_2:41318295-41318314 Msa0070680:intron 65.0%
! CTTGGATCGGTCGGTGGTGG+TGG - chr1_2:41318298-41318317 Msa0070680:intron 65.0%
CTCAGGATGGCCGGAGCCTG+AGG + chr1_2:41317853-41317872 None:intergenic 70.0%
CTCAGGCTCCGGCCATCCTG+AGG - chr1_2:41317851-41317870 Msa0070680:CDS 70.0%
GCAGCGTGCACACCGAGCCA+AGG + chr1_2:41318457-41318476 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 41317107 41320049 41317107 ID=Msa0070680;Name=Msa0070680
chr1_2 mRNA 41317107 41320049 41317107 ID=Msa0070680-mRNA-1;Parent=Msa0070680;Name=Msa0070680-mRNA-1;_AED=0.01;_eAED=0.01;_QI=118|1|1|1|1|1|3|339|364
chr1_2 exon 41317107 41317973 41317107 ID=Msa0070680-mRNA-1:exon:12626;Parent=Msa0070680-mRNA-1
chr1_2 exon 41318643 41318981 41318643 ID=Msa0070680-mRNA-1:exon:12625;Parent=Msa0070680-mRNA-1
chr1_2 exon 41319704 41320049 41319704 ID=Msa0070680-mRNA-1:exon:12624;Parent=Msa0070680-mRNA-1
chr1_2 five_prime_UTR 41319932 41320049 41319932 ID=Msa0070680-mRNA-1:five_prime_utr;Parent=Msa0070680-mRNA-1
chr1_2 CDS 41319704 41319931 41319704 ID=Msa0070680-mRNA-1:cds;Parent=Msa0070680-mRNA-1
chr1_2 CDS 41318643 41318981 41318643 ID=Msa0070680-mRNA-1:cds;Parent=Msa0070680-mRNA-1
chr1_2 CDS 41317446 41317973 41317446 ID=Msa0070680-mRNA-1:cds;Parent=Msa0070680-mRNA-1
chr1_2 three_prime_UTR 41317107 41317445 41317107 ID=Msa0070680-mRNA-1:three_prime_utr;Parent=Msa0070680-mRNA-1
Gene Sequence

>Msa0070680

ATGGGTAGGCTTGCACCATTATCAGAAGAACCTATCAATGAAGAAAATGAAAGAAAATTATCAAAATATAACTCTAAGAAAGGTTTTCAGTTATGGATGAATTGGAGTTGGATCAAGACTCATTTTTCACTTGCTTTCAACAAAAAATCTAACCTCAAGATGCTTCTTAGTGTTCTTGGTTGTCCTCTTTTTCCTGTTCCTATTCATCCTAAATCACCCCTCAATGAGGTTTCGTCATCGGCACAGTATATTATACAACAATTCACAGCTGCAACAGGGTGTAGGAAGTTGGAGGGGACAGTGAAGAATGTGTTTGCAACCGGAAAAGTGACAATGGTTGTCGTGGACGAGCTTGGATCGGTCGGTGGTGGTGGTGTTAATGGAACTTCAGAAAAAGGGTGTTTTGTGATGTGGCAAATGGTTCCTGATAATTGGCAGATAGAGATTGTTGTTGGTGGCCAAAAGGTTCTTGCTGGTAGTAACGGCACTATTGCTTGGCGTCACACACCTTGGCTCGGTGTGCACGCTGCTAAAGGCGGCGTTCGTCCTCTTCGCCGTGCACTTCAGGGACTAGACCCTTTAGCAGTATCAGCTGTATTCAGTGCAGCACAGCACATGGGGGAGAAACAAATCAACGGCGTAGATTGTTTCGTACTAAAACTCTCAGCCGATCAAAAGGACCTAATCGAACGAAGCGACAACACGGCCGAGATGATAAAGCATGCTATCTTTGGTTATTTTAGTCAAAGAAGTGGACTTTTAGTGCACTTAGAGGACTCTTATTTGACAAGGATTCAAGCAATAGGGACAAATCCTACATATTGGGAAACAACAATGTCAACAAAAATTGAAGACTATAGAGATGTTGATGGTGTTATGATTGCACATGCTGGATCAACAACTGTGATGATAACAAGGTTTGGTGATAATCTTAAGGATGGACCTGCTATTACTAGGTTGGAAGAGTCATGGACTATTGATGATGTTGCATTTAATGTTCAAGGGTTGTCTATTGATTGTTTCTTACCACCTCAGGAACTTAACAAAGATTGTCCACAACAACATCTTGATTGGAGATCATCATTGCATAGATGA
Protein sequence

>Msa0070680

MGRLAPLSEEPINEENERKLSKYNSKKGFQLWMNWSWIKTHFSLAFNKKSNLKMLLSVLGCPLFPVPIHPKSPLNEVSSSAQYIIQQFTAATGCRKLEGTVKNVFATGKVTMVVVDELGSVGGGGVNGTSEKGCFVMWQMVPDNWQIEIVVGGQKVLAGSNGTIAWRHTPWLGVHAAKGGVRPLRRALQGLDPLAVSAVFSAAQHMGEKQINGVDCFVLKLSADQKDLIERSDNTAEMIKHAIFGYFSQRSGLLVHLEDSYLTRIQAIGTNPTYWETTMSTKIEDYRDVDGVMIAHAGSTTVMITRFGDNLKDGPAITRLEESWTIDDVAFNVQGLSIDCFLPPQELNKDCPQQHLDWRSSLHR*