AlfalfaGEDB | Msa0086710

M. sativa cultivar zhongmu-4 / Msa0086710

KEGG: K19882
GO: -
PFAM: PF03283.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086710	KEH43697.1	91.228	114	9	1	1	113	10	123	9.67e-69	222

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086710	sp\|B9DFR3\|PAE9_ARATH	60.177	113	44	1	1	113	8	119	2.16e-40	142

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086710	A0A072VPF8	91.228	114	9	1	1	113	10	123	4.62e-69	222

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0086710	Msa0133570	0.837060	6.493837e-57	-8.615850e-47
Msa0086710	Msa0181520	0.895103	1.317314e-75	-8.615850e-47
Msa0086710	Msa0863800	0.805431	1.339273e-49	-8.615850e-47
Msa0086710	Msa0985550	0.807905	4.021533e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086710	MtrunA17_Chr1g0203101	91.228	114	9	1	1	113	10	123	2.19e-72	221
Msa0086710	MtrunA17_Chr1g0203121	91.228	114	9	1	1	113	10	123	3.07e-72	221
Msa0086710	MtrunA17_Chr7g0271231	56.818	88	38	0	26	113	53	140	5.82e-31	114
Msa0086710	MtrunA17_Chr1g0193381	53.933	89	41	0	25	113	49	137	1.08e-29	110
Msa0086710	MtrunA17_Chr7g0270931	52.222	90	43	0	24	113	47	136	5.77e-29	108
Msa0086710	MtrunA17_Chr1g0193761	50.562	89	44	0	25	113	36	124	4.03e-28	106
Msa0086710	MtrunA17_Chr5g0408881	54.651	86	39	0	28	113	28	113	1.21e-27	105
Msa0086710	MtrunA17_Chr4g0069571	50.562	89	44	0	25	113	54	142	2.64e-27	104
Msa0086710	MtrunA17_Chr8g0369651	45.133	113	61	1	1	113	1	112	3.30e-27	103
Msa0086710	MtrunA17_Chr7g0238491	47.872	94	48	1	21	113	48	141	6.71e-25	97.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086710	AT5G23870.1	60.177	113	44	1	1	113	8	119	1.29e-41	142
Msa0086710	AT5G23870.4	60.177	113	44	1	1	113	8	119	1.29e-41	142
Msa0086710	AT5G23870.2	60.177	113	44	1	1	113	8	119	1.68e-41	142
Msa0086710	AT5G23870.3	60.177	113	44	1	1	113	8	119	2.20e-41	142
Msa0086710	AT1G57590.1	54.255	94	43	0	20	113	71	164	1.11e-30	114
Msa0086710	AT3G05910.2	51.579	95	45	1	19	113	43	136	2.44e-30	110
Msa0086710	AT3G62060.2	55.294	85	38	0	29	113	55	139	4.84e-30	112
Msa0086710	AT3G62060.1	55.294	85	38	0	29	113	55	139	5.75e-30	111
Msa0086710	AT5G26670.1	54.023	87	40	0	27	113	51	137	8.76e-30	111
Msa0086710	AT3G05910.1	55.172	87	38	1	27	113	51	136	1.54e-29	110

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Find 60 sgRNAs with CRISPR-Local

Find 381 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAGAGAAGCGTGAA+AGG	0.456845	1_2:+67453389	None:intergenic
AAATCGAGGCTTCATTGATT+TGG	0.300538	1_2:+67453227	None:intergenic
AAATTTATAGTTTGTTTGGA+TGG	0.442549	1_2:-67452780	Msa0086710:intron
AACAATGCTACCGTTAATCC+AGG	0.520859	1_2:-67447942	Msa0086710:CDS
AAGCCTCGATTTCAATTACG+TGG	0.580072	1_2:-67453216	Msa0086710:five_prime_UTR
AAGGAGTGTAGTGTAGTGCG+CGG	0.696531	1_2:+67453450	None:intergenic
AATCCACGTAATTGAAATCG+AGG	0.627604	1_2:+67453213	None:intergenic
AATTTATAGTTTGTTTGGAT+GGG	0.489051	1_2:-67452779	Msa0086710:intron
ACCAAATATGAGAACTTCAA+GGG	0.586887	1_2:-67447975	Msa0086710:CDS
ACCCACCATCAAACAGAATA+TGG	0.318619	1_2:+67453583	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATCCATATTCTGTTTGA+TGG	-	chr1_2:67447894-67447913	Msa0086710:CDS	30.0%
GAAGAAAAATTGGATGAGAA+AGG	+	chr1_2:67447964-67447983	None:intergenic	30.0%
GATTTGGAAAGAAACGTAAA+CGG	+	chr1_2:67448302-67448321	None:intergenic	30.0%
GAAGAAATGGTAAATCACAA+TGG	+	chr1_2:67448324-67448343	None:intergenic	30.0%
ATTGTTGTATCTAATCACAG+TGG	-	chr1_2:67448522-67448541	Msa0086710:intron	30.0%
TGATGTGAATGAAGTGAAAA+TGG	-	chr1_2:67449275-67449294	Msa0086710:intron	30.0%
CGGTAAAATTTCCTTCAATA+AGG	-	chr1_2:67449368-67449387	Msa0086710:intron	30.0%
AGAAAGTTAATCCATTGTGT+TGG	-	chr1_2:67449780-67449799	Msa0086710:intron	30.0%
AAATAAAGGAGAGAGAAAAG+TGG	-	chr1_2:67450039-67450058	Msa0086710:intron	30.0%
AATGGTGAGTGAAAAAGTTA+CGG	-	chr1_2:67450290-67450309	Msa0086710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	67447893	67453611	67447893	ID=Msa0086710;Name=Msa0086710
chr1_2	mRNA	67447893	67453611	67447893	ID=Msa0086710-mRNA-1;Parent=Msa0086710;Name=Msa0086710-mRNA-1;_AED=0.02;_eAED=0.02;_QI=606\|1\|0.66\|1\|0.5\|1\|3\|0\|129
chr1_2	exon	67447893	67448072	67447893	ID=Msa0086710-mRNA-1:exon:22898;Parent=Msa0086710-mRNA-1
chr1_2	exon	67452710	67452792	67452710	ID=Msa0086710-mRNA-1:exon:22897;Parent=Msa0086710-mRNA-1
chr1_2	exon	67452879	67453611	67452879	ID=Msa0086710-mRNA-1:exon:22896;Parent=Msa0086710-mRNA-1
chr1_2	five_prime_UTR	67453006	67453611	67453006	ID=Msa0086710-mRNA-1:five_prime_utr;Parent=Msa0086710-mRNA-1
chr1_2	CDS	67452879	67453005	67452879	ID=Msa0086710-mRNA-1:cds;Parent=Msa0086710-mRNA-1
chr1_2	CDS	67452710	67452792	67452710	ID=Msa0086710-mRNA-1:cds;Parent=Msa0086710-mRNA-1
chr1_2	CDS	67447893	67448072	67447893	ID=Msa0086710-mRNA-1:cds;Parent=Msa0086710-mRNA-1

Gene Sequence

>Msa0086710

ATGAAATTCGCGGTGGCGTTCGCATTGTTGTATCTAATCACAGTGGAGGCATGGCGTGTTCACTCACAAGAACAGAAGATTTATGTGAACATGACGCTCGTTAACAACGCACGTGAAACCGGTGCTTTTTGTTTGGATGGGAGCTTACCGGCATATCACCTGGACAGAGGATTTGGAGCTGGTAAAGACAATTGGCTTCTACAATTTGAGGGAGGTGGTTGGTGCAATGACTTGAAATCATGCTTGGAGAGAGCGCAAACCCGCAGGGGTTCAACAAATTACATGACCAAATATGAGAACTTCAAGGGTATTTTAAGCAACAATGCTACCGTTAATCCAGGTAAATCAATATCTGCTCTGTTTTTTCTTTTCTTTTCATCTTTCATCTGA

Protein sequence

>Msa0086710

MKFAVAFALLYLITVEAWRVHSQEQKIYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGKDNWLLQFEGGGWCNDLKSCLERAQTRRGSTNYMTKYENFKGILSNNATVNPGKSISALFFLFFSSFI*