AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0087810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087810 MtrunA17_Chr1g0204791 91.057 123 9 2 1 122 1 122 8.53e-77 223
Msa0087810 MtrunA17_Chr5g0432501 37.069 116 66 2 14 122 21 136 2.59e-21 82.8
Msa0087810 MtrunA17_Chr8g0342581 35.200 125 58 4 20 122 6 129 8.07e-15 65.9
Msa0087810 MtrunA17_Chr4g0019101 42.105 76 39 2 47 122 92 162 1.22e-13 63.5
Msa0087810 MtrunA17_Chr4g0019111 42.188 64 35 1 59 122 16 77 7.78e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087810 AT3G22820.1 46.729 107 54 1 16 122 4 107 1.15e-27 98.2
Msa0087810 AT4G14723.1 49.524 105 40 3 19 122 17 109 6.24e-27 96.7
Msa0087810 AT2G30370.2 44.828 116 51 4 16 122 45 156 7.87e-23 87.4
Msa0087810 AT2G30370.3 44.828 116 51 4 16 122 55 166 1.02e-22 87.4
Msa0087810 AT2G30370.1 51.190 84 33 2 47 122 147 230 1.06e-21 86.3

Find 77 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCCTTCCCTCTTT+AGG 0.211079 1_2:+68758367 Msa0087810:five_prime_UTR
CACTTGCAACGCCATGCTTC+TGG 0.251321 1_2:-68759013 None:intergenic
TGCTCTGAGGTTTGAATTGT+TGG 0.274165 1_2:+68759261 Msa0087810:three_prime_UTR
TTTGGGACTTGGGTTGGGTT+TGG 0.275308 1_2:+68759370 Msa0087810:three_prime_UTR
CATTGGGAATGTAAGATGTT+TGG 0.278233 1_2:+68759352 Msa0087810:three_prime_UTR
CCAATGAAGAAGCCTTAAAA+AGG 0.292236 1_2:-68759335 None:intergenic
AAGCAATGGTGGTGGTGTTT+AGG 0.302399 1_2:-68758633 None:intergenic
ATTGGGAATGTAAGATGTTT+GGG 0.318124 1_2:+68759353 Msa0087810:three_prime_UTR
AACACCACCACCATTGCTTC+TGG 0.327365 1_2:+68758637 Msa0087810:CDS
AGCAATGGTGGTGGTGTTTA+GGG 0.333049 1_2:-68758632 None:intergenic
ATGATTAAACCGGGGTGAAC+CGG 0.350078 1_2:-68758974 None:intergenic
AGATGTTTGGGACTTGGGTT+GGG 0.351631 1_2:+68759365 Msa0087810:three_prime_UTR
GCGGAGCAGAAGAAGGTTGC+TGG 0.381489 1_2:+68758881 Msa0087810:CDS
CAGAAGAAGGTTGCTGGGCC+AGG 0.385682 1_2:+68758887 Msa0087810:CDS
AAACGTTGCCAGAACCGAAC+AGG 0.401440 1_2:+68759137 Msa0087810:three_prime_UTR
ATGTAAGATGTTTGGGACTT+GGG 0.402279 1_2:+68759360 Msa0087810:three_prime_UTR
TACTACTATGTACAATAGAT+AGG 0.411982 1_2:-68759419 None:intergenic
CTCAAGGCGTATGATTAAAC+CGG 0.413396 1_2:-68758984 None:intergenic
GAGAAGAAATGAACCTAAAG+AGG 0.416992 1_2:-68758380 None:intergenic
GGGTGAACCGGAACGTGAAC+CGG 0.417630 1_2:-68758962 None:intergenic
AAGATGTTTGGGACTTGGGT+TGG 0.421077 1_2:+68759364 Msa0087810:three_prime_UTR
AATGTAAGATGTTTGGGACT+TGG 0.424168 1_2:+68759359 Msa0087810:three_prime_UTR
GAGGTTTGAATTGTTGGACT+TGG 0.433808 1_2:+68759267 Msa0087810:three_prime_UTR
TGAGAGTGAGAAAGAGAGAA+TGG 0.434630 1_2:-68758520 None:intergenic
ATGTCAACCGGTTCACGTTC+CGG 0.448654 1_2:+68758955 Msa0087810:CDS
AACAGTGAAGCATCAAGAAA+TGG 0.452869 1_2:+68758817 Msa0087810:CDS
GTTTGAATTGTTGGACTTGG+TGG 0.458192 1_2:+68759270 Msa0087810:three_prime_UTR
AAGCATCAAGAAATGGAAAA+CGG 0.474883 1_2:+68758824 Msa0087810:CDS
CTCTTCTTCCACGGTGATGT+CGG 0.479412 1_2:-68758851 None:intergenic
GGTTCCACTTGTGTAACATT+GGG 0.482486 1_2:+68759445 Msa0087810:three_prime_UTR
TTAATACATGCATATCATAT+AGG 0.484606 1_2:-68759232 None:intergenic
AGCATCAAGAAATGGAAAAC+GGG 0.486636 1_2:+68758825 Msa0087810:CDS
CGTGTAGATCCAAGTGTGAT+TGG 0.513041 1_2:+68758924 Msa0087810:CDS
GAAGCATGGCGTTGCAAGTG+TGG 0.519320 1_2:+68759016 Msa0087810:CDS
AGAGGTGGCGGAGCAGAAGA+AGG 0.519433 1_2:+68758874 Msa0087810:CDS
TTGGTGTAACCCATGTCAAC+CGG 0.519681 1_2:+68758943 Msa0087810:CDS
GGAACGTGAACCGGTTGACA+TGG 0.526123 1_2:-68758953 None:intergenic
AGTGAGAAAGAGAGAATGGA+AGG 0.528050 1_2:-68758516 None:intergenic
GCCACCTCTCTCTTCTTCCA+CGG 0.532769 1_2:-68758860 None:intergenic
GGGTTCCACTTGTGTAACAT+TGG 0.534793 1_2:+68759444 Msa0087810:three_prime_UTR
CGGAGCAGAAGAAGGTTGCT+GGG 0.544379 1_2:+68758882 Msa0087810:CDS
GTATTATTAAATGTTACTGC+AGG 0.545034 1_2:-68758487 None:intergenic
TCTATTGTACATAGTAGTAG+AGG 0.545608 1_2:+68759423 Msa0087810:three_prime_UTR
GTGGAAGAAGAGAGAGGTGG+CGG 0.545993 1_2:+68758862 Msa0087810:CDS
TTGTGGTGCGAGAAGAGTTA+TGG 0.548135 1_2:-68758703 None:intergenic
TAATTATCAATTCATCATCA+AGG 0.551277 1_2:-68759055 None:intergenic
AGAGTACAGAGAAGATGAAG+AGG 0.551498 1_2:-68758551 None:intergenic
AACTTGAACTTACTAGGTTG+TGG 0.559169 1_2:-68758720 None:intergenic
AGAAGAAGGTTGCTGGGCCA+GGG 0.561033 1_2:+68758888 Msa0087810:CDS
ACTACTATGTACAATAGATA+GGG 0.569442 1_2:-68759418 None:intergenic
AATGCCCAATGTTACACAAG+TGG 0.571707 1_2:-68759449 None:intergenic
TCAAGGCGTATGATTAAACC+GGG 0.574666 1_2:-68758983 None:intergenic
AAGAGAGAATGGAAGGTGTG+TGG 0.576934 1_2:-68758509 None:intergenic
ATGCTTCTGGATAATACTCA+AGG 0.599588 1_2:-68759000 None:intergenic
GAAATGAACCTAAAGAGGGA+AGG 0.600783 1_2:-68758375 None:intergenic
TGGGTTACACCAATCACACT+TGG 0.609884 1_2:-68758933 None:intergenic
AAGCATGGCGTTGCAAGTGT+GGG 0.610762 1_2:+68759017 Msa0087810:CDS
AGAAGAAATGAACCTAAAGA+GGG 0.612120 1_2:-68758379 None:intergenic
CACGACGGAGGTGTTGACCC+TGG 0.613267 1_2:-68758905 None:intergenic
GAACGTGAACCGGTTGACAT+GGG 0.614728 1_2:-68758952 None:intergenic
GTACGCACATATTGATGAGG+TGG 0.617360 1_2:-68758602 None:intergenic
TTGAGTATTATCCAGAAGCA+TGG 0.618402 1_2:+68759002 Msa0087810:CDS
CTATTGTACATAGTAGTAGA+GGG 0.632056 1_2:+68759424 Msa0087810:three_prime_UTR
AACGTTGCCAGAACCGAACA+GGG 0.633112 1_2:+68759138 Msa0087810:three_prime_UTR
TGGGTAATAACCAGAAGCAA+TGG 0.633930 1_2:-68758647 None:intergenic
GTTCACGTTCCGGTTCACCC+CGG 0.635691 1_2:+68758965 Msa0087810:CDS
ATAACCAGAAGCAATGGTGG+TGG 0.639918 1_2:-68758641 None:intergenic
GTAATAACCAGAAGCAATGG+TGG 0.650622 1_2:-68758644 None:intergenic
AATGGTGGTGGTGTTTAGGG+TGG 0.653961 1_2:-68758629 None:intergenic
GCAGTACGCACATATTGATG+AGG 0.669919 1_2:-68758605 None:intergenic
GTATTAATGATGTTGCTCTG+AGG 0.673923 1_2:+68759248 Msa0087810:three_prime_UTR
ACCGTGGAAGAAGAGAGAGG+TGG 0.701228 1_2:+68758859 Msa0087810:CDS
ATCACCGTGGAAGAAGAGAG+AGG 0.712922 1_2:+68758856 Msa0087810:CDS
CACTTGGATCTACACGACGG+AGG 0.750576 1_2:-68758917 None:intergenic
TCACACTTGGATCTACACGA+CGG 0.754283 1_2:-68758920 None:intergenic
ACGGGTCTCCGACATCACCG+TGG 0.754489 1_2:+68758843 Msa0087810:CDS
CAAGGCGTATGATTAAACCG+GGG 0.811542 1_2:-68758982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATCAATTCATCATCA+AGG - chr1_2:68759058-68759077 None:intergenic 20.0%
!! TTAATACATGCATATCATAT+AGG - chr1_2:68759235-68759254 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr1_2:68759307-68759326 Msa0087810:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr1_2:68759323-68759342 Msa0087810:three_prime_UTR 20.0%
!! TAATTATCAATTCATCATCA+AGG - chr1_2:68759058-68759077 None:intergenic 20.0%
!! TTAATACATGCATATCATAT+AGG - chr1_2:68759235-68759254 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr1_2:68759307-68759326 Msa0087810:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr1_2:68759323-68759342 Msa0087810:three_prime_UTR 20.0%
! GTATTATTAAATGTTACTGC+AGG - chr1_2:68758490-68758509 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr1_2:68758673-68758692 None:intergenic 25.0%
!! TGAAAAAAGTATGAAGAAAG+TGG - chr1_2:68758761-68758780 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG - chr1_2:68759118-68759137 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr1_2:68759181-68759200 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr1_2:68759180-68759199 Msa0087810:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr1_2:68759181-68759200 Msa0087810:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr1_2:68759197-68759216 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr1_2:68759198-68759217 None:intergenic 25.0%
! ACTACTATGTACAATAGATA+GGG - chr1_2:68759421-68759440 None:intergenic 25.0%
! TACTACTATGTACAATAGAT+AGG - chr1_2:68759422-68759441 None:intergenic 25.0%
! GTATTATTAAATGTTACTGC+AGG - chr1_2:68758490-68758509 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr1_2:68758673-68758692 None:intergenic 25.0%
!! TGAAAAAAGTATGAAGAAAG+TGG - chr1_2:68758761-68758780 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG - chr1_2:68759118-68759137 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr1_2:68759181-68759200 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr1_2:68759180-68759199 Msa0087810:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr1_2:68759181-68759200 Msa0087810:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr1_2:68759197-68759216 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr1_2:68759198-68759217 None:intergenic 25.0%
! ACTACTATGTACAATAGATA+GGG - chr1_2:68759421-68759440 None:intergenic 25.0%
! TACTACTATGTACAATAGAT+AGG - chr1_2:68759422-68759441 None:intergenic 25.0%
AGAAGAAATGAACCTAAAGA+GGG - chr1_2:68758382-68758401 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr1_2:68758391-68758410 Msa0087810:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr1_2:68758669-68758688 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr1_2:68758670-68758689 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr1_2:68758729-68758748 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr1_2:68758785-68758804 Msa0087810:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr1_2:68758794-68758813 Msa0087810:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr1_2:68758824-68758843 Msa0087810:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr1_2:68759196-68759215 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr1_2:68759336-68759355 Msa0087810:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr1_2:68759353-68759372 Msa0087810:three_prime_UTR 30.0%
TCTATTGTACATAGTAGTAG+AGG + chr1_2:68759423-68759442 Msa0087810:three_prime_UTR 30.0%
CTATTGTACATAGTAGTAGA+GGG + chr1_2:68759424-68759443 Msa0087810:three_prime_UTR 30.0%
AGAAGAAATGAACCTAAAGA+GGG - chr1_2:68758382-68758401 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr1_2:68758391-68758410 Msa0087810:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr1_2:68758669-68758688 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr1_2:68758670-68758689 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr1_2:68758729-68758748 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr1_2:68758785-68758804 Msa0087810:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr1_2:68758794-68758813 Msa0087810:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr1_2:68758824-68758843 Msa0087810:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr1_2:68759196-68759215 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr1_2:68759336-68759355 Msa0087810:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr1_2:68759353-68759372 Msa0087810:three_prime_UTR 30.0%
TCTATTGTACATAGTAGTAG+AGG + chr1_2:68759423-68759442 Msa0087810:three_prime_UTR 30.0%
CTATTGTACATAGTAGTAGA+GGG + chr1_2:68759424-68759443 Msa0087810:three_prime_UTR 30.0%
GAGAAGAAATGAACCTAAAG+AGG - chr1_2:68758383-68758402 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr1_2:68758723-68758742 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr1_2:68758817-68758836 Msa0087810:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr1_2:68758825-68758844 Msa0087810:CDS 35.0%
ATGCTTCTGGATAATACTCA+AGG - chr1_2:68759003-68759022 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr1_2:68759002-68759021 Msa0087810:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr1_2:68759248-68759267 Msa0087810:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr1_2:68759338-68759357 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr1_2:68759335-68759354 Msa0087810:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr1_2:68759352-68759371 Msa0087810:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr1_2:68759359-68759378 Msa0087810:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr1_2:68759360-68759379 Msa0087810:three_prime_UTR 35.0%
GAGAAGAAATGAACCTAAAG+AGG - chr1_2:68758383-68758402 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr1_2:68758723-68758742 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr1_2:68758817-68758836 Msa0087810:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr1_2:68758825-68758844 Msa0087810:CDS 35.0%
ATGCTTCTGGATAATACTCA+AGG - chr1_2:68759003-68759022 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr1_2:68759002-68759021 Msa0087810:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr1_2:68759248-68759267 Msa0087810:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr1_2:68759338-68759357 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr1_2:68759335-68759354 Msa0087810:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr1_2:68759352-68759371 Msa0087810:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr1_2:68759359-68759378 Msa0087810:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr1_2:68759360-68759379 Msa0087810:three_prime_UTR 35.0%
GAAATGAACCTAAAGAGGGA+AGG - chr1_2:68758378-68758397 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr1_2:68758431-68758450 Msa0087810:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr1_2:68758519-68758538 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr1_2:68758523-68758542 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr1_2:68758554-68758573 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr1_2:68758647-68758666 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr1_2:68758650-68758669 None:intergenic 40.0%
TCAAGGCGTATGATTAAACC+GGG - chr1_2:68758986-68759005 None:intergenic 40.0%
CTCAAGGCGTATGATTAAAC+CGG - chr1_2:68758987-68759006 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr1_2:68759154-68759173 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr1_2:68759261-68759280 Msa0087810:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr1_2:68759267-68759286 Msa0087810:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr1_2:68759270-68759289 Msa0087810:three_prime_UTR 40.0%
GGTTCCACTTGTGTAACATT+GGG + chr1_2:68759445-68759464 Msa0087810:three_prime_UTR 40.0%
AATGCCCAATGTTACACAAG+TGG - chr1_2:68759452-68759471 None:intergenic 40.0%
GAAATGAACCTAAAGAGGGA+AGG - chr1_2:68758378-68758397 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr1_2:68758431-68758450 Msa0087810:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr1_2:68758519-68758538 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr1_2:68758523-68758542 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr1_2:68758554-68758573 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr1_2:68758647-68758666 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr1_2:68758650-68758669 None:intergenic 40.0%
TCAAGGCGTATGATTAAACC+GGG - chr1_2:68758986-68759005 None:intergenic 40.0%
CTCAAGGCGTATGATTAAAC+CGG - chr1_2:68758987-68759006 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr1_2:68759154-68759173 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr1_2:68759261-68759280 Msa0087810:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr1_2:68759267-68759286 Msa0087810:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr1_2:68759270-68759289 Msa0087810:three_prime_UTR 40.0%
GGTTCCACTTGTGTAACATT+GGG + chr1_2:68759445-68759464 Msa0087810:three_prime_UTR 40.0%
AATGCCCAATGTTACACAAG+TGG - chr1_2:68759452-68759471 None:intergenic 40.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr1_2:68758512-68758531 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr1_2:68758605-68758624 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr1_2:68758608-68758627 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr1_2:68758635-68758654 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr1_2:68758636-68758655 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr1_2:68758644-68758663 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr1_2:68758706-68758725 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr1_2:68758923-68758942 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr1_2:68758924-68758943 Msa0087810:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr1_2:68758936-68758955 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr1_2:68758943-68758962 Msa0087810:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr1_2:68758977-68758996 None:intergenic 45.0%
CAAGGCGTATGATTAAACCG+GGG - chr1_2:68758985-68759004 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr1_2:68759148-68759167 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr1_2:68759364-68759383 Msa0087810:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr1_2:68759365-68759384 Msa0087810:three_prime_UTR 45.0%
GGGTTCCACTTGTGTAACAT+TGG + chr1_2:68759444-68759463 Msa0087810:three_prime_UTR 45.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr1_2:68758512-68758531 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr1_2:68758605-68758624 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr1_2:68758608-68758627 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr1_2:68758635-68758654 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr1_2:68758636-68758655 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr1_2:68758644-68758663 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr1_2:68758706-68758725 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr1_2:68758923-68758942 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr1_2:68758924-68758943 Msa0087810:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr1_2:68758936-68758955 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr1_2:68758943-68758962 Msa0087810:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr1_2:68758977-68758996 None:intergenic 45.0%
CAAGGCGTATGATTAAACCG+GGG - chr1_2:68758985-68759004 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr1_2:68759148-68759167 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr1_2:68759364-68759383 Msa0087810:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr1_2:68759365-68759384 Msa0087810:three_prime_UTR 45.0%
GGGTTCCACTTGTGTAACAT+TGG + chr1_2:68759444-68759463 Msa0087810:three_prime_UTR 45.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr1_2:68758367-68758386 Msa0087810:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr1_2:68758632-68758651 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr1_2:68758637-68758656 Msa0087810:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr1_2:68758854-68758873 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr1_2:68758856-68758875 Msa0087810:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr1_2:68758955-68758974 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr1_2:68758955-68758974 Msa0087810:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr1_2:68759017-68759036 Msa0087810:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr1_2:68759137-68759156 Msa0087810:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr1_2:68759138-68759157 Msa0087810:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr1_2:68759370-68759389 Msa0087810:three_prime_UTR 50.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr1_2:68758367-68758386 Msa0087810:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr1_2:68758632-68758651 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr1_2:68758637-68758656 Msa0087810:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr1_2:68758854-68758873 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr1_2:68758856-68758875 Msa0087810:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr1_2:68758955-68758974 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr1_2:68758955-68758974 Msa0087810:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr1_2:68759017-68759036 Msa0087810:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr1_2:68759137-68759156 Msa0087810:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr1_2:68759138-68759157 Msa0087810:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr1_2:68759370-68759389 Msa0087810:three_prime_UTR 50.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr1_2:68758859-68758878 Msa0087810:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr1_2:68758863-68758882 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr1_2:68758862-68758881 Msa0087810:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr1_2:68758882-68758901 Msa0087810:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr1_2:68758888-68758907 Msa0087810:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr1_2:68758920-68758939 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr1_2:68758956-68758975 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr1_2:68759016-68759035 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr1_2:68759016-68759035 Msa0087810:CDS 55.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr1_2:68758859-68758878 Msa0087810:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr1_2:68758863-68758882 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr1_2:68758862-68758881 Msa0087810:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr1_2:68758882-68758901 Msa0087810:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr1_2:68758888-68758907 Msa0087810:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr1_2:68758920-68758939 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr1_2:68758956-68758975 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr1_2:68759016-68759035 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr1_2:68759016-68759035 Msa0087810:CDS 55.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr1_2:68758874-68758893 Msa0087810:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr1_2:68758881-68758900 Msa0087810:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr1_2:68758887-68758906 Msa0087810:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr1_2:68758965-68758984 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr1_2:68758965-68758984 Msa0087810:CDS 60.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr1_2:68758874-68758893 Msa0087810:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr1_2:68758881-68758900 Msa0087810:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr1_2:68758887-68758906 Msa0087810:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr1_2:68758965-68758984 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr1_2:68758965-68758984 Msa0087810:CDS 60.0%
ACGGGTCTCCGACATCACCG+TGG + chr1_2:68758843-68758862 Msa0087810:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr1_2:68758908-68758927 None:intergenic 65.0%
ACGGGTCTCCGACATCACCG+TGG + chr1_2:68758843-68758862 Msa0087810:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr1_2:68758908-68758927 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 68758343 68759482 68758343 ID=Msa0087810;Name=Msa0087810
chr1_2 mRNA 68758343 68759482 68758343 ID=Msa0087810-mRNA-1;Parent=Msa0087810;Name=Msa0087810-mRNA-1;_AED=0.00;_eAED=0.00;_QI=272|1|1|1|1|1|2|422|122
chr1_2 exon 68758343 68758729 68758343 ID=Msa0087810-mRNA-1:exon:23545;Parent=Msa0087810-mRNA-1
chr1_2 exon 68758807 68759482 68758807 ID=Msa0087810-mRNA-1:exon:23546;Parent=Msa0087810-mRNA-1
chr1_2 five_prime_UTR 68758343 68758614 68758343 ID=Msa0087810-mRNA-1:five_prime_utr;Parent=Msa0087810-mRNA-1
chr1_2 CDS 68758615 68758729 68758615 ID=Msa0087810-mRNA-1:cds;Parent=Msa0087810-mRNA-1
chr1_2 CDS 68758807 68759060 68758807 ID=Msa0087810-mRNA-1:cds;Parent=Msa0087810-mRNA-1
chr1_2 three_prime_UTR 68759061 68759482 68759061 ID=Msa0087810-mRNA-1:three_prime_utr;Parent=Msa0087810-mRNA-1
Gene Sequence

>Msa0087810

ATGTGCGTACTGCGCCACCCTAAACACCACCACCATTGCTTCTGGTTATTACCCACCTTCATTTTTCTCTTGTTTTCTTCTTCTTCAGCCATAACTCTTCTCGCACCACAACCTAGTAGTAGTGGAACAGTGAAGCATCAAGAAATGGAAAACGGGTCTCCGACATCACCGTGGAAGAAGAGAGAGGTGGCGGAGCAGAAGAAGGTTGCTGGGCCAGGGTCAACACCTCCGTCGTGTAGATCCAAGTGTGATTGGTGTAACCCATGTCAACCGGTTCACGTTCCGGTTCACCCCGGTTTAATCATACGCCTTGAGTATTATCCAGAAGCATGGCGTTGCAAGTGTGGGAATAAGTATTTTTTGCCTTGA
Protein sequence

>Msa0087810

MCVLRHPKHHHHCFWLLPTFIFLLFSSSSAITLLAPQPSSSGTVKHQEMENGSPTSPWKKREVAEQKKVAGPGSTPPSCRSKCDWCNPCQPVHVPVHPGLIIRLEYYPEAWRCKCGNKYFLP*