AlfalfaGEDB | Msa0091620

M. sativa cultivar zhongmu-4 / Msa0091620

KEGG: -
GO: -
PFAM: PF08387.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0091620	RHN45187.1	53.237	139	61	2	121	259	175	309	2.38e-40	152

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0091620	A0A396H2R0	53.237	139	61	2	121	259	175	309	1.14e-40	152

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0091620	Msa0115360	0.866560	2.608096e-65	-8.615850e-47
Msa0091620	Msa0484750	0.889323	2.684299e-73	-8.615850e-47
Msa0091620	Msa0769390	0.802977	4.343044e-49	-8.615850e-47
Msa0091620	Msa1222970	0.804907	1.724342e-49	-8.615850e-47
Msa0091620	Msa1367080	0.809421	1.907832e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0091620	MtrunA17_Chr7g0227631	53.237	139	61	2	121	259	175	309	2.19e-44	152
Msa0091620	MtrunA17_Chr7g0227651	53.237	139	61	2	121	259	249	383	2.48e-44	154
Msa0091620	MtrunA17_Chr7g0227641	39.506	243	100	7	59	260	130	366	1.17e-43	152
Msa0091620	MtrunA17_Chr7g0227831	51.613	124	56	2	135	258	1	120	1.35e-39	134
Msa0091620	MtrunA17_Chr7g0226481	46.386	166	79	4	100	258	187	349	2.36e-37	135
Msa0091620	MtrunA17_Chr7g0226471	46.154	143	76	1	117	258	4	146	2.12e-33	119
Msa0091620	MtrunA17_Chr1g0166251	45.833	144	69	4	120	258	246	385	5.39e-31	118
Msa0091620	MtrunA17_Chr7g0227841	41.830	153	80	3	112	262	33	178	2.41e-30	112
Msa0091620	MtrunA17_Chr7g0227851	41.667	144	76	3	115	258	245	380	8.65e-27	107
Msa0091620	MtrunA17_Chr7g0262091	41.606	137	77	3	123	258	256	390	1.99e-24	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 141 sgRNAs with CRISPR-Local

Find 550 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACAACATCTGCAGTT+GGG	0.492080	1_2:-73736015	None:intergenic
AAAACACTCTCAAGTGTAAG+TGG	0.538714	1_2:-73728581	None:intergenic
AAAAGAGCAGCATTTGGTGC+CGG	0.560258	1_2:+73736273	Msa0091620:three_prime_UTR
AAAAGCTTGACATTGTTGCT+TGG	0.511442	1_2:-73728980	None:intergenic
AAACTCTCCATTACTTTCAT+TGG	0.331462	1_2:-73728818	None:intergenic
AAACTCTGATGCGACTATTG+TGG	0.622881	1_2:+73734354	Msa0091620:CDS
AAATGATCGGATGGCCGTTG+TGG	0.640364	1_2:-73728454	None:intergenic
AACTTAACTCACCTCGAGCT+TGG	0.445621	1_2:+73733924	Msa0091620:CDS
AAGATAACTGATTTACTACT+CGG	0.551137	1_2:+73728790	Msa0091620:five_prime_UTR
AATAAATCTGAACCTGTGAA+TGG	0.532809	1_2:-73731560	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGGAAGAAAAGATAGAATG+TGG	-	chr1_2:73728419-73728438	None:intergenic	30.0%
GATATCTTTATAATGGCATC+CGG	-	chr1_2:73728493-73728512	None:intergenic	30.0%
AAACTCTCCATTACTTTCAT+TGG	-	chr1_2:73728821-73728840	None:intergenic	30.0%
AAGTAGAAACAAGTAGAAAG+TGG	-	chr1_2:73729239-73729258	None:intergenic	30.0%
ATAGAAATTTCACAAAGGCT+GGG	-	chr1_2:73729412-73729431	None:intergenic	30.0%
AATAGAAATTTCACAAAGGC+TGG	-	chr1_2:73729413-73729432	None:intergenic	30.0%
ATAAACATGTACATTGTTCG+AGG	-	chr1_2:73729525-73729544	None:intergenic	30.0%
TATGAGAATTGCGTTTAGTA+TGG	+	chr1_2:73729672-73729691	Msa0091620:intron	30.0%
TTAATGCAATTGCAGCATAA+TGG	+	chr1_2:73730283-73730302	Msa0091620:intron	30.0%
TGATAAATTTGACGCTCTTT+GGG	+	chr1_2:73730326-73730345	Msa0091620:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	73728292	73736314	73728292	ID=Msa0091620;Name=Msa0091620
chr1_2	mRNA	73728292	73736314	73728292	ID=Msa0091620-mRNA-1;Parent=Msa0091620;Name=Msa0091620-mRNA-1;_AED=0.23;_eAED=0.26;_QI=859\|0.33\|0.57\|0.85\|0.16\|0.42\|7\|511\|269
chr1_2	exon	73728292	73729211	73728292	ID=Msa0091620-mRNA-1:exon:25919;Parent=Msa0091620-mRNA-1
chr1_2	exon	73729911	73729977	73729911	ID=Msa0091620-mRNA-1:exon:25920;Parent=Msa0091620-mRNA-1
chr1_2	exon	73730913	73731042	73730913	ID=Msa0091620-mRNA-1:exon:25921;Parent=Msa0091620-mRNA-1
chr1_2	exon	73731512	73731607	73731512	ID=Msa0091620-mRNA-1:exon:25922;Parent=Msa0091620-mRNA-1
chr1_2	exon	73733894	73734040	73733894	ID=Msa0091620-mRNA-1:exon:25923;Parent=Msa0091620-mRNA-1
chr1_2	exon	73734140	73734422	73734140	ID=Msa0091620-mRNA-1:exon:25924;Parent=Msa0091620-mRNA-1
chr1_2	exon	73735778	73736314	73735778	ID=Msa0091620-mRNA-1:exon:25925;Parent=Msa0091620-mRNA-1
chr1_2	five_prime_UTR	73728292	73729150	73728292	ID=Msa0091620-mRNA-1:five_prime_utr;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73729151	73729211	73729151	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73729911	73729977	73729911	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73730913	73731042	73730913	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73731512	73731607	73731512	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73733894	73734040	73733894	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73734140	73734422	73734140	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	CDS	73735778	73735803	73735778	ID=Msa0091620-mRNA-1:cds;Parent=Msa0091620-mRNA-1
chr1_2	three_prime_UTR	73735804	73736314	73735804	ID=Msa0091620-mRNA-1:three_prime_utr;Parent=Msa0091620-mRNA-1

Gene Sequence

>Msa0091620

ATGCAAATATTGTTGTGTCCAAGGGGCTCTCCTACATGTCAAATTTTATTTGTTGAGGAAGTAAAGAAAGGTGAAGATTGTTTAGCTCATTTATTGAAGATTAATGAAATACTCTTCACTGGGCCTTTGTTCAGCCCATGTATGGGTGAGACAAGGCTTGGTTGTTGTTGGTTTAGGATCAGCCCTAAACTATCAAGATTAACCAACAAGATCAGGCAGCCAATAGTGGTCAAGCTGCACAAAAATCGGCTTGCTATGAGGATCGTAATCCTTGATCTACAAGGTGAAATTCCGTTGGTTCTGAAACCATTCACAGGTTCAGATTTATTGCAGTTTTCAGCCAAAGCAATTTCGATGCACAAGCAACAATGTCACACTTTTCGTAACTTAACTCACCTCGAGCTTGGGCTTATCTTTGCGTCCAATTGGCATACCAATTTGAACTGCTTGATAGAAGTGCTACGAGATTGTCCAAAGCTTGAGAATCTTACCCTTCATGAGTTTGATGGACATGGAGTTGGTGACGACATGTGCAAAAAACCACAAAATGTATTGGTTCCGGAATGTCTTTCCTCTCAATTAAAAACATGCACTCTTATAGGTTATAAAGGCACGTACGCTGGGATTCAATTTATAGCCTATATTTTAAAGAATGCGAAATTACTGAACACTGTGACGATTAAAGCCTCACAAGGGGATTTAAACATAAGGCACCAAACTCTGATGCGACTATTGTGGTGTCCAAGATGCGCTCCTACATGTCGAATTTTATTTGTTGAGGAAGGTTCAGCCCAGGTATGGGTGGGATAA

Protein sequence

>Msa0091620

MQILLCPRGSPTCQILFVEEVKKGEDCLAHLLKINEILFTGPLFSPCMGETRLGCCWFRISPKLSRLTNKIRQPIVVKLHKNRLAMRIVILDLQGEIPLVLKPFTGSDLLQFSAKAISMHKQQCHTFRNLTHLELGLIFASNWHTNLNCLIEVLRDCPKLENLTLHEFDGHGVGDDMCKKPQNVLVPECLSSQLKTCTLIGYKGTYAGIQFIAYILKNAKLLNTVTIKASQGDLNIRHQTLMRLLWCPRCAPTCRILFVEEGSAQVWVG*