AlfalfaGEDB | Msa1333480

M. sativa cultivar zhongmu-4 / Msa1333480

KEGG: K12735
GO: GO:0000413 GO:0001932 GO:0003674 GO:0003676 GO:0003723 GO:0003755 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031399 GO:0032268 GO:0036211 GO:0042325 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051174 GO:0051246 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901407 GO:1901564 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00160.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333480	sp\|Q6Q151\|CYP59_ARATH	75.227	440	94	5	1	434	1	431	0.0	671

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333480	A0A072U025	97.048	542	13	1	1	539	1	542	0.0	1074

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa1333480	0.847041	1.485983e-59	-8.615850e-47
Msa0001940	Msa1333480	0.815646	8.310528e-52	-8.615850e-47
Msa0002580	Msa1333480	0.815114	1.091190e-51	-8.615850e-47
Msa0003700	Msa1333480	0.836485	9.104141e-57	-8.615850e-47
Msa0003890	Msa1333480	0.854301	1.348258e-61	-8.615850e-47
Msa0005890	Msa1333480	0.835638	1.493256e-56	-8.615850e-47
Msa0006110	Msa1333480	0.846071	2.733387e-59	-8.615850e-47
Msa0006380	Msa1333480	0.800422	1.452000e-48	-8.615850e-47
Msa0009180	Msa1333480	0.846324	2.333457e-59	-8.615850e-47
Msa0010110	Msa1333480	0.801257	9.808285e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333480	MtrunA17_Chr8g0357591	97.048	542	13	1	1	539	1	542	0.0	1074
Msa1333480	MtrunA17_Chr6g0484281	95.572	542	21	1	1	539	1	542	0.0	1034
Msa1333480	MtrunA17_Chr7g0266721	41.772	158	83	2	3	158	12	162	2.54e-35	130
Msa1333480	MtrunA17_Chr8g0351571	39.394	165	91	2	1	163	1	158	1.30e-32	122
Msa1333480	MtrunA17_Chr5g0401141	39.157	166	92	2	3	166	347	505	4.19e-31	127
Msa1333480	MtrunA17_Chr2g0321111	39.623	159	87	2	2	158	463	614	1.31e-30	126
Msa1333480	MtrunA17_Chr2g0288371	41.317	167	87	5	3	165	15	174	6.95e-26	111
Msa1333480	MtrunA17_Chr7g0260441	37.179	156	75	5	12	153	23	169	4.02e-22	100
Msa1333480	MtrunA17_Chr3g0145501	39.157	166	79	6	9	161	25	181	7.58e-22	93.6
Msa1333480	MtrunA17_Chr7g0274841	34.810	158	81	5	10	154	18	166	4.39e-20	87.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333480	AT1G53720.1	75.227	440	94	5	1	434	1	431	0.0	671
Msa1333480	AT1G53720.2	84.570	337	49	1	1	334	1	337	0.0	601
Msa1333480	AT1G53780.2	49.327	223	90	4	78	297	4	206	3.85e-50	182
Msa1333480	AT1G53780.1	46.829	205	86	4	96	297	1	185	8.06e-40	153
Msa1333480	AT1G53780.4	46.829	205	86	4	96	297	1	185	8.06e-40	153
Msa1333480	AT1G53780.3	46.829	205	86	4	96	297	1	185	8.62e-40	153
Msa1333480	AT2G36130.1	43.125	160	82	3	3	160	12	164	3.89e-38	138
Msa1333480	AT1G01940.1	39.394	165	91	2	1	163	1	158	3.09e-32	122
Msa1333480	AT3G44600.1	40.881	159	85	2	2	158	477	628	8.57e-31	127
Msa1333480	AT5G67530.1	38.994	159	88	2	7	163	350	501	1.01e-28	120

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Find 154 sgRNAs with CRISPR-Local

Find 564 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAAGCCCGGATGATG+GGG	0.591110	8_4:-53638266	Msa1333480:three_prime_UTR
AAACCCAGGAGAACTTGAAG+AGG	0.634019	8_4:-53640921	Msa1333480:CDS
AAACTTACCTGTAGTAGCAT+AGG	0.573597	8_4:-53637867	Msa1333480:three_prime_UTR
AAAGACATAGAAAGGCATAT+GGG	0.577796	8_4:-53638598	Msa1333480:CDS
AACATAGAAGGACATGATAC+CGG	0.564254	8_4:-53638189	Msa1333480:three_prime_UTR
AACGGGGTGGAGATAAAGCA+AGG	0.636526	8_4:-53638973	Msa1333480:intron
AACTATCCTCATCCTCTAGA+AGG	0.416633	8_4:-53638159	Msa1333480:three_prime_UTR
AACTGTGGTCACAATACATG+CGG	0.633456	8_4:-53639246	Msa1333480:CDS
AAGATGAATCTAACGGTAGT+TGG	0.540734	8_4:+53645778	None:intergenic
AAGGACAATAATGGCCAACG+GGG	0.743691	8_4:-53638989	Msa1333480:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACTTGTAGATAGAATCAC+CGG	+	chr8_4:53638216-53638235	None:intergenic	30.0%
TGCAGTAGAAATTAGAAAAG+AGG	-	chr8_4:53638406-53638425	Msa1333480:CDS	30.0%
GCAGTAGAAATTAGAAAAGA+GGG	-	chr8_4:53638407-53638426	Msa1333480:CDS	30.0%
TAGAAAAGAGGGTTGAAAAT+AGG	-	chr8_4:53638418-53638437	Msa1333480:CDS	30.0%
TTGTTTATGATAGAACACTC+TGG	-	chr8_4:53638581-53638600	Msa1333480:CDS	30.0%
GTCATAAAGGATTAGATTCT+AGG	-	chr8_4:53638874-53638893	Msa1333480:intron	30.0%
TTTACCGTCAATGTTAGAAT+TGG	+	chr8_4:53638901-53638920	None:intergenic	30.0%
ATATGAGGTTTCTCAATGAA+TGG	-	chr8_4:53639064-53639083	Msa1333480:CDS	30.0%
ACGGATTTAACTCATCTATT+AGG	-	chr8_4:53639123-53639142	Msa1333480:intron	30.0%
GCATGAGAAATAATCTTGAT+TGG	-	chr8_4:53639337-53639356	Msa1333480:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	53637848	53645857	53637848	ID=Msa1333480;Name=Msa1333480
chr8_4	mRNA	53637848	53645857	53637848	ID=Msa1333480-mRNA-1;Parent=Msa1333480;Name=Msa1333480-mRNA-1;_AED=0.12;_eAED=0.12;_QI=94\|1\|1\|1\|1\|1\|14\|527\|546
chr8_4	exon	53637848	53638888	53637848	ID=Msa1333480-mRNA-1:exon:16501;Parent=Msa1333480-mRNA-1
chr8_4	exon	53638974	53639103	53638974	ID=Msa1333480-mRNA-1:exon:16500;Parent=Msa1333480-mRNA-1
chr8_4	exon	53639233	53639329	53639233	ID=Msa1333480-mRNA-1:exon:16499;Parent=Msa1333480-mRNA-1
chr8_4	exon	53639810	53639850	53639810	ID=Msa1333480-mRNA-1:exon:16498;Parent=Msa1333480-mRNA-1
chr8_4	exon	53640397	53640452	53640397	ID=Msa1333480-mRNA-1:exon:16497;Parent=Msa1333480-mRNA-1
chr8_4	exon	53640540	53640586	53640540	ID=Msa1333480-mRNA-1:exon:16496;Parent=Msa1333480-mRNA-1
chr8_4	exon	53640707	53640784	53640707	ID=Msa1333480-mRNA-1:exon:16495;Parent=Msa1333480-mRNA-1
chr8_4	exon	53640874	53640996	53640874	ID=Msa1333480-mRNA-1:exon:16494;Parent=Msa1333480-mRNA-1
chr8_4	exon	53642008	53642107	53642008	ID=Msa1333480-mRNA-1:exon:16493;Parent=Msa1333480-mRNA-1
chr8_4	exon	53642213	53642304	53642213	ID=Msa1333480-mRNA-1:exon:16492;Parent=Msa1333480-mRNA-1
chr8_4	exon	53643974	53644015	53643974	ID=Msa1333480-mRNA-1:exon:16491;Parent=Msa1333480-mRNA-1
chr8_4	exon	53644175	53644292	53644175	ID=Msa1333480-mRNA-1:exon:16490;Parent=Msa1333480-mRNA-1
chr8_4	exon	53645474	53645578	53645474	ID=Msa1333480-mRNA-1:exon:16489;Parent=Msa1333480-mRNA-1
chr8_4	exon	53645666	53645857	53645666	ID=Msa1333480-mRNA-1:exon:16488;Parent=Msa1333480-mRNA-1
chr8_4	five_prime_UTR	53645764	53645857	53645764	ID=Msa1333480-mRNA-1:five_prime_utr;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53645666	53645763	53645666	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53645474	53645578	53645474	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53644175	53644292	53644175	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53643974	53644015	53643974	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53642213	53642304	53642213	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53642008	53642107	53642008	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53640874	53640996	53640874	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53640707	53640784	53640707	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53640540	53640586	53640540	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53640397	53640452	53640397	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53639810	53639850	53639810	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53639233	53639329	53639233	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53638974	53639103	53638974	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	CDS	53638375	53638888	53638375	ID=Msa1333480-mRNA-1:cds;Parent=Msa1333480-mRNA-1
chr8_4	three_prime_UTR	53637848	53638374	53637848	ID=Msa1333480-mRNA-1:three_prime_utr;Parent=Msa1333480-mRNA-1

Gene Sequence

>Msa1333480

ATGTCAGTACTGATGGTGACGAGTTTAGGCGACATGGTTATCGACTTACACACACAAAAATGTCCCTTAACCTGCAAAAACTTCTTGAAGCTTTGCAAGATCAAGTATTACAATGGATGTTTATTTCATACTGTTCAAAAGGATTTTACTGCTCAGACTGGTGATCCTACTGGAACTGGCACTGCCGGTGATTCTATCTACAAGTTTCTTTATGGTGACCAAGCTCGGTTTTTCAATGATGAGATTCATATTGATCTAAAGCATTCTAAGACTGGAACTGTTGCCATGGCTAGTGCAGGAGAGAATTTGAATGCTTCACAGTTCTATATAACTCTGCGTGATTACCTTGATGGAAAGCACACAGTTTTTGGTGAGGTGGCTGAAGGATTTGAGACTTTGACTAGAATTAATGAGGCTTATGCCGATGGAAAGGGAAGACCATTCAAAAATATCCGAATCAAGCACACTTACATCCTGGAAGATCCTTTTGATGATCCTCCTCAGCTACCTGAGTTCATTCCTGAAGCTTCACCAGAAGGAAAACCTAAAGACGAGGTTGACGATGAAGTCCGACTTGAAGATGATTGGGTGCCCATGGATGAACAATTAAACCCAGGAGAACTTGAAGAGGTTATTAGATCAAAGGAAGCACATTCTAGGGCAGTTGTGCTTGAGAGTATTGGGGATATTCCTGATGCTGAGGTTAAACCTCCAGATAATGTACTTTTTGTCTGTAAATTGAATCCGGTTACTGAGGATGAGGACTTGCACACAATATTTTCACGCTTTGGAACTGTATCATCGGCTGAAATCATTCGTGATCACAAGACTGGTGACAGTTTATGCTATGCTTTTATAGAGTTCGAAGACAAACTGGCATGTGAGCAGGCATATTTTAAGATGGATAATGCTTTGATTGATGATCGAAGGATACATGTGGATTTTAGTCAAAGTGTTTCAAAACTGTGGTCACAATACATGCGGAAAGATAATAAGGGTGGTGGTTGCTTCAAATGTGGTTCCACAGATCATATTGCTAAGGATTGTACTGGAGATGCTACAATGAAACAACCCACAAAATTTATCATGAAGGACAATAATGGCCAACGGGGTGGAGATAAAGCAAGATACGAAATGGTTTTTGATGGAGATAACACGGAAAGTCCAAAGAGAGAAACGAAACACCAGAGATATGAAAGGGATGACGGATATGATAAAAAAGGTCGGAAGGAAAATTTTAAAGATGATAGTCATAGAGGTCGTAGGGATCAGGAGATGGAAGGCTCGAATAACAGAGATAGGCACCGCGATAGAAGCAGAGGGCTTGAAGGAAATGGAGATGATAAAGCGCGACTGGAAAAAGGTGCAAGAGATCCGGATTTTCATGCCGAGAAGAAAGACATAGAAAGGCATATGGGAAGGAGACGTCGAGATGATGACTACCGGCGGAAAGATGAGCTAGACTCGAGAAAAAGAGACTTAGATGATAGATACACAGAAAGACGGGCTAGTCGAGATGATAGGAGAAAAACAGATGACAGCCATTTGGATAGGAGGGATGACAGAGATTATAGGAAGAGAACTGAAGACAGTGGAAGGCAAGATGTGAAGGTTGACTGGAAGGAGATGTGCGATGATTGA

Protein sequence

>Msa1333480

MSVLMVTSLGDMVIDLHTQKCPLTCKNFLKLCKIKYYNGCLFHTVQKDFTAQTGDPTGTGTAGDSIYKFLYGDQARFFNDEIHIDLKHSKTGTVAMASAGENLNASQFYITLRDYLDGKHTVFGEVAEGFETLTRINEAYADGKGRPFKNIRIKHTYILEDPFDDPPQLPEFIPEASPEGKPKDEVDDEVRLEDDWVPMDEQLNPGELEEVIRSKEAHSRAVVLESIGDIPDAEVKPPDNVLFVCKLNPVTEDEDLHTIFSRFGTVSSAEIIRDHKTGDSLCYAFIEFEDKLACEQAYFKMDNALIDDRRIHVDFSQSVSKLWSQYMRKDNKGGGCFKCGSTDHIAKDCTGDATMKQPTKFIMKDNNGQRGGDKARYEMVFDGDNTESPKRETKHQRYERDDGYDKKGRKENFKDDSHRGRRDQEMEGSNNRDRHRDRSRGLEGNGDDKARLEKGARDPDFHAEKKDIERHMGRRRRDDDYRRKDELDSRKRDLDDRYTERRASRDDRRKTDDSHLDRRDDRDYRKRTEDSGRQDVKVDWKEMCDD*