AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0094730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094730 MtrunA17_Chr4g0069171 98.305 118 2 0 1 118 1 118 3.61e-83 238
Msa0094730 MtrunA17_Chr7g0260981 97.458 118 3 0 1 118 1 118 2.49e-82 236
Msa0094730 MtrunA17_Chr1g0194141 68.644 118 37 0 1 118 1 118 7.17e-60 179
Msa0094730 MtrunA17_Chr4g0024721 57.895 114 48 0 5 118 4 117 4.48e-48 149
Msa0094730 MtrunA17_Chr2g0322571 56.140 114 50 0 5 118 4 117 2.24e-46 145
Msa0094730 MtrunA17_Chr2g0288531 55.752 113 50 0 6 118 5 117 6.33e-46 144
Msa0094730 MtrunA17_Chr4g0056301 56.250 112 49 0 7 118 6 117 2.13e-45 143
Msa0094730 MtrunA17_Chr4g0019141 49.107 112 55 2 6 115 5 116 9.23e-32 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094730 AT3G06420.1 72.174 115 32 0 4 118 5 119 3.50e-61 183
Msa0094730 AT3G15580.1 70.435 115 34 0 4 118 1 115 1.12e-60 182
Msa0094730 AT2G05630.1 55.652 115 51 0 4 118 3 117 7.13e-48 149
Msa0094730 AT4G04620.3 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa0094730 AT4G04620.2 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa0094730 AT4G04620.1 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa0094730 AT4G21980.1 57.018 114 49 0 5 118 4 117 4.82e-46 145
Msa0094730 AT1G62040.1 57.018 114 49 0 5 118 4 117 5.84e-46 144
Msa0094730 AT4G21980.2 57.018 114 49 0 5 118 19 132 8.25e-46 145
Msa0094730 AT4G16520.2 54.386 114 52 0 5 118 4 117 6.61e-45 142
Msa0094730 AT4G16520.1 54.386 114 52 0 5 118 4 117 6.61e-45 142
Msa0094730 AT2G05630.2 55.556 108 48 0 11 118 54 161 6.46e-44 141
Msa0094730 AT3G60640.1 53.097 113 53 0 6 118 5 117 1.54e-42 136
Msa0094730 AT2G45170.2 50.847 118 58 0 1 118 1 118 3.31e-42 135
Msa0094730 AT2G45170.1 50.847 118 58 0 1 118 1 118 3.31e-42 135
Msa0094730 AT4G16520.3 52.273 88 42 0 31 118 30 117 5.78e-32 109
Msa0094730 AT1G62040.2 53.846 91 42 0 5 95 4 94 6.25e-31 105

Find 69 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTCTGTGCATATATTT+TGG 0.140548 1_2:+78087002 Msa0094730:three_prime_UTR
CAACAAATCGTTGTTTATAT+TGG 0.212724 1_2:-78086375 Msa0094740:three_prime_UTR
GTCTACTTGCAGCTAATTAT+TGG 0.237931 1_2:-78086771 Msa0094740:intron
TATGAGTGTAACAATCTAAT+TGG 0.265742 1_2:-78086490 Msa0094740:three_prime_UTR
GATGTTGAGGTTACAGTTCT+TGG 0.266368 1_2:-78086646 Msa0094740:CDS
TACTTCTTCTTGTCCAATTC+AGG 0.278998 1_2:-78084867 None:intergenic
CATCCTCCTCCTTGTAAGTT+TGG 0.322568 1_2:-78086185 Msa0094740:three_prime_UTR
AATTGAAGTTGTAATGAAAA+TGG 0.327561 1_2:-78084453 None:intergenic
AGCAATCCAAGCATCAGTTT+TGG 0.342021 1_2:-78086960 Msa0094740:CDS
CAGCTTGGTACCTGGGCATT+GGG 0.361123 1_2:-78084170 None:intergenic
TAGGACAGACCTACCTGAAT+TGG 0.369195 1_2:+78084854 Msa0094730:CDS
CGTTGTTTATATTGGAGATT+CGG 0.378593 1_2:-78086367 Msa0094740:three_prime_UTR
GAGAGAGAATTCTTGTTTGA+AGG 0.384970 1_2:-78084565 None:intergenic
CTGTTGGGTTAGCTAAAGTT+AGG 0.387750 1_2:+78087040 Msa0094730:three_prime_UTR
ATTACTGCACAAAATAAATC+TGG 0.389938 1_2:+78086717 Msa0094730:three_prime_UTR
GAGTATTGTTTGAACTCAAC+AGG 0.391562 1_2:-78087172 Msa0094740:intron
CTCCTTGTAAGTTTGGTAGA+TGG 0.391844 1_2:-78086178 Msa0094740:three_prime_UTR
TCAGCTTGGTACCTGGGCAT+TGG 0.400093 1_2:-78084171 None:intergenic
CAAATCAATATCATATATCA+TGG 0.403889 1_2:+78084530 Msa0094730:exon
TTGTTACACTCATAGATTGC+AGG 0.409345 1_2:+78086499 Msa0094730:three_prime_UTR
AATAATACGGGAACTCGATC+GGG 0.413583 1_2:-78084730 None:intergenic
TACAGCAGTGAGAAAACATT+TGG 0.420898 1_2:+78086225 Msa0094730:CDS
TCATCATTGAGAAATATTCT+AGG 0.422511 1_2:+78084835 Msa0094730:CDS
CACAATTGACAAATACTTGA+AGG 0.442560 1_2:-78086285 Msa0094740:three_prime_UTR
ACCTTTCCAAAACTGATGCT+TGG 0.443667 1_2:+78086954 Msa0094730:intron
AGTAGGCTACGTTTGCCTCC+TGG 0.446454 1_2:+78085705 Msa0094730:CDS
AAGATGCATTTACCAGTTTG+AGG 0.454421 1_2:-78085762 None:intergenic
GTAGGCTACGTTTGCCTCCT+GGG 0.455739 1_2:+78085706 Msa0094730:CDS
GAGTAGCGGACTAGCGGACT+TGG 0.457546 1_2:+78086455 Msa0094730:three_prime_UTR
AAATAATACGGGAACTCGAT+CGG 0.458795 1_2:-78084731 None:intergenic
TAATTTCACCAGTCAATACC+TGG 0.466177 1_2:-78086698 Msa0094740:CDS
GAGAAAACATTTGGTTAACT+TGG 0.487241 1_2:+78086234 Msa0094730:exon
ATACAAGAATGTAGTTGCAT+TGG 0.491129 1_2:-78086582 Msa0094740:three_prime_UTR
AAACTGTATCCCAAAGGAAA+AGG 0.507112 1_2:-78087093 Msa0094740:CDS
CTCCATCTACCAAACTTACA+AGG 0.518610 1_2:+78086176 Msa0094730:CDS
GTAATGAAAATGGAAGGAAT+TGG 0.521825 1_2:-78084443 None:intergenic
CAAACTTACAAGGAGGAGGA+TGG 0.523873 1_2:+78086186 Msa0094730:CDS
TTCAAACAAGAATTCTCTCT+CGG 0.525359 1_2:+78084567 Msa0094730:CDS
GTTCCTCGAGACATGTCTGT+TGG 0.526742 1_2:+78085660 Msa0094730:CDS
CATTTGCTTTGTAGATGTTG+AGG 0.531213 1_2:-78086659 Msa0094740:CDS
AAAGGAAAAGGTACCGACTC+GGG 0.545865 1_2:-78087081 Msa0094740:CDS
AACAATCGCACCCAATGCCC+AGG 0.547604 1_2:+78084160 Msa0094730:five_prime_UTR
TCTTCTTGTCCAATTCAGGT+AGG 0.548533 1_2:-78084863 None:intergenic
TAGATACCCCAGGTATTGAC+TGG 0.568960 1_2:+78086690 Msa0094730:three_prime_UTR
CAAAGGAAAAGGTACCGACT+CGG 0.570075 1_2:-78087082 Msa0094740:CDS
TATTGATTTGAATTATGTGT+TGG 0.578463 1_2:-78084517 None:intergenic
GAAGTTGTAATGAAAATGGA+AGG 0.580623 1_2:-78084449 None:intergenic
GCGATTGTTCGAGATGAACA+TGG 0.580865 1_2:-78084146 None:intergenic
AAATCAATATCATATATCAT+GGG 0.582392 1_2:+78084531 Msa0094730:exon
AAAATAAACAGAGCTTTCCC+AGG 0.586278 1_2:-78085723 None:intergenic
CTACCAAACTTACAAGGAGG+AGG 0.590671 1_2:+78086182 Msa0094730:CDS
CTTGTAAGTTTGGTAGATGG+AGG 0.594122 1_2:-78086175 Msa0094740:three_prime_UTR
GAATCCAGATGAACGACGCA+AGG 0.597013 1_2:+78084681 Msa0094730:intron
AAACTTACAAGGAGGAGGAT+GGG 0.597052 1_2:+78086187 Msa0094730:CDS
AATTTCACCAGTCAATACCT+GGG 0.612530 1_2:-78086697 Msa0094740:CDS
CATAGATTGCAGGATTGACA+AGG 0.619050 1_2:+78086509 Msa0094730:three_prime_UTR
TGAGGTTACAGTTCTTGGAG+TGG 0.629632 1_2:-78086641 Msa0094740:CDS
ATACCTGGGGTATCTAGTGA+AGG 0.632705 1_2:-78086683 Msa0094740:CDS
CAGATAAAACTGTATCCCAA+AGG 0.633902 1_2:-78087099 Msa0094740:CDS
TGTCCTTCACTAGATACCCC+AGG 0.633913 1_2:+78086680 Msa0094730:three_prime_UTR
TGTTAAGAGTAGCGGACTAG+CGG 0.641760 1_2:+78086449 Msa0094730:three_prime_UTR
ACATGGAGAAGCTTTAGCAT+CGG 0.648150 1_2:-78084129 None:intergenic
TGTGCCATGTTCATGACTTG+AGG 0.660953 1_2:+78086741 Msa0094730:three_prime_UTR
AGCAAGATATCTGCCCGAGT+CGG 0.666491 1_2:+78087068 Msa0094730:three_prime_UTR
ATAAACAGAGCTTTCCCAGG+AGG 0.685967 1_2:-78085720 None:intergenic
AGTGCCTCAAGTCATGAACA+TGG 0.700230 1_2:-78086745 Msa0094740:CDS
TGTCCAACAGACATGTCTCG+AGG 0.712779 1_2:-78085663 None:intergenic
ATTTCACCAGTCAATACCTG+GGG 0.717112 1_2:-78086696 Msa0094740:CDS
CATCTACCAAACTTACAAGG+AGG 0.745667 1_2:+78086179 Msa0094730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTAATTAACTATTTTTG+TGG + chr1_2:78084333-78084352 Msa0094730:intron 10.0%
!!! ATATATTATAGAATTCTTTT+TGG + chr1_2:78085487-78085506 Msa0094730:intron 10.0%
!! AAATCAATATCATATATCAT+GGG + chr1_2:78084531-78084550 Msa0094730:exon 15.0%
!!! TATTTTATATTGAAATGTGA+GGG - chr1_2:78084619-78084638 None:intergenic 15.0%
!!! TTATTTTATATTGAAATGTG+AGG - chr1_2:78084620-78084639 None:intergenic 15.0%
!! ATTGATTGAGAAAAAAAAAA+TGG - chr1_2:78084764-78084783 None:intergenic 15.0%
!! ATTTATCTTATTCTTATTGA+AGG + chr1_2:78084812-78084831 Msa0094730:intron 15.0%
!!! ACAAAATTGACAAATTTTAT+AGG - chr1_2:78085012-78085031 None:intergenic 15.0%
!! TCTTAACATGAAAAAAAATA+TGG - chr1_2:78086437-78086456 Msa0094740:three_prime_UTR 15.0%
!! AATTGAAGTTGTAATGAAAA+TGG - chr1_2:78084456-78084475 None:intergenic 20.0%
!!! TATTGATTTGAATTATGTGT+TGG - chr1_2:78084520-78084539 None:intergenic 20.0%
!! CAAATCAATATCATATATCA+TGG + chr1_2:78084530-78084549 Msa0094730:exon 20.0%
!!! ATGTTTTCATTTTGCATTTT+TGG + chr1_2:78084889-78084908 Msa0094730:intron 20.0%
!!! AATGATGATGTTGAATAATT+AGG - chr1_2:78084921-78084940 None:intergenic 20.0%
!!! AAATTCAAAATCAGCTTTTA+AGG - chr1_2:78085436-78085455 None:intergenic 20.0%
!!! AATACCAATTTTGATTTGAA+GGG + chr1_2:78085598-78085617 Msa0094730:intron 20.0%
!! AGAAAACAAAATATGAGATT+AGG - chr1_2:78085624-78085643 None:intergenic 20.0%
!!! TTATTCACATTTTAAGTAGT+AGG + chr1_2:78085688-78085707 Msa0094730:CDS 20.0%
!! GTTATAGTAGTTATTAGTTA+AGG + chr1_2:78085804-78085823 Msa0094730:intron 20.0%
!! CAAAAAGTTATCATATAACT+TGG + chr1_2:78087126-78087145 Msa0094730:three_prime_UTR 20.0%
! AATGGAGGAGAAAAATAATA+CGG - chr1_2:78084746-78084765 None:intergenic 25.0%
! GATTGAGAAAAAAAAAATGG+AGG - chr1_2:78084761-78084780 None:intergenic 25.0%
! TCATCATTGAGAAATATTCT+AGG + chr1_2:78084835-78084854 Msa0094730:CDS 25.0%
!! TTTGTCAATTTTGTATGTGT+TGG + chr1_2:78085018-78085037 Msa0094730:intron 25.0%
!! TTGTCAATTTTGTATGTGTT+GGG + chr1_2:78085019-78085038 Msa0094730:intron 25.0%
! TAAAGCATGGAAAATACAAA+TGG - chr1_2:78085328-78085347 None:intergenic 25.0%
!! TTTATAAGCTCTAGTTGATT+AGG - chr1_2:78085397-78085416 None:intergenic 25.0%
!!! AAGCTGATTTTGAATTTATG+TGG + chr1_2:78085439-78085458 Msa0094730:intron 25.0%
!!! GAATACCAATTTTGATTTGA+AGG + chr1_2:78085597-78085616 Msa0094730:intron 25.0%
!! TTTTCTCTCTTTTAGATACT+TGG + chr1_2:78085638-78085657 Msa0094730:intron 25.0%
! TGCAAATGCATAATAAATCA+TGG + chr1_2:78086027-78086046 Msa0094730:intron 25.0%
! GCAAATGCATAATAAATCAT+GGG + chr1_2:78086028-78086047 Msa0094730:intron 25.0%
! CAAATGCATAATAAATCATG+GGG + chr1_2:78086029-78086048 Msa0094730:intron 25.0%
! AAATGCATAATAAATCATGG+GGG + chr1_2:78086030-78086049 Msa0094730:intron 25.0%
! CAACAAATCGTTGTTTATAT+TGG - chr1_2:78086378-78086397 Msa0094740:three_prime_UTR 25.0%
!!! TTTTTTCATGTTAAGAGTAG+CGG + chr1_2:78086441-78086460 Msa0094730:three_prime_UTR 25.0%
! TATGAGTGTAACAATCTAAT+TGG - chr1_2:78086493-78086512 Msa0094740:three_prime_UTR 25.0%
! ATTACTGCACAAAATAAATC+TGG + chr1_2:78086717-78086736 Msa0094730:three_prime_UTR 25.0%
!! TATTGTCTGTGCATATATTT+TGG + chr1_2:78087002-78087021 Msa0094730:three_prime_UTR 25.0%
!! GTTATATGATAACTTTTTGC+AGG - chr1_2:78087125-78087144 Msa0094740:intron 25.0%
AATAGAACAAATAGCATGCA+AGG - chr1_2:78084205-78084224 None:intergenic 30.0%
AAGTCAATGTCAATCAACAA+CGG - chr1_2:78084373-78084392 None:intergenic 30.0%
TGACTTACACGTTAAATTGA+CGG + chr1_2:78084387-78084406 Msa0094730:intron 30.0%
GTAATGAAAATGGAAGGAAT+TGG - chr1_2:78084446-78084465 None:intergenic 30.0%
GAAGTTGTAATGAAAATGGA+AGG - chr1_2:78084452-78084471 None:intergenic 30.0%
TTCAAACAAGAATTCTCTCT+CGG + chr1_2:78084567-78084586 Msa0094730:CDS 30.0%
!!! GGATTTTGAGATGAAAACAA+AGG - chr1_2:78084598-78084617 None:intergenic 30.0%
! ATTGCATTAATGTTTGCTGA+TGG + chr1_2:78084649-78084668 Msa0094730:intron 30.0%
ATGGAGGAGAAAAATAATAC+GGG - chr1_2:78084745-78084764 None:intergenic 30.0%
!! AGTAATGAGAGTTGATAAGA+TGG + chr1_2:78085098-78085117 Msa0094730:intron 30.0%
AAGACTGAGAAAAGCTATAA+GGG + chr1_2:78085149-78085168 Msa0094730:intron 30.0%
TGATGCATGATAGAATGTTA+TGG + chr1_2:78085217-78085236 Msa0094730:intron 30.0%
AACTAGAGCTTATAAAGCTT+GGG + chr1_2:78085401-78085420 Msa0094730:intron 30.0%
!! CTTTTTGGTGATAGCAATAT+AGG + chr1_2:78085502-78085521 Msa0094730:intron 30.0%
TTAGAGCATGTTTAGTATCA+CGG + chr1_2:78085915-78085934 Msa0094730:intron 30.0%
GAGAAAACATTTGGTTAACT+TGG + chr1_2:78086234-78086253 Msa0094730:exon 30.0%
CACAATTGACAAATACTTGA+AGG - chr1_2:78086288-78086307 Msa0094740:three_prime_UTR 30.0%
CGTTGTTTATATTGGAGATT+CGG - chr1_2:78086370-78086389 Msa0094740:three_prime_UTR 30.0%
ATACAAGAATGTAGTTGCAT+TGG - chr1_2:78086585-78086604 Msa0094740:three_prime_UTR 30.0%
! GTTACACTTTGTTGTGAAAT+TGG - chr1_2:78086927-78086946 Msa0094740:intron 30.0%
! GAGAGAGAATTCTTGTTTGA+AGG - chr1_2:78084568-78084587 None:intergenic 35.0%
AAATAATACGGGAACTCGAT+CGG - chr1_2:78084734-78084753 None:intergenic 35.0%
TACTTCTTCTTGTCCAATTC+AGG - chr1_2:78084870-78084889 None:intergenic 35.0%
! CAATTTTGTATGTGTTGGGA+TGG + chr1_2:78085023-78085042 Msa0094730:intron 35.0%
!! AATGAGAGTTGATAAGATGG+AGG + chr1_2:78085101-78085120 Msa0094730:intron 35.0%
!! ATGAGAGTTGATAAGATGGA+GGG + chr1_2:78085102-78085121 Msa0094730:intron 35.0%
GAAGACTGAGAAAAGCTATA+AGG + chr1_2:78085148-78085167 Msa0094730:intron 35.0%
GGAAACTATTAAGAAGAGTG+TGG + chr1_2:78085170-78085189 Msa0094730:intron 35.0%
GAGTGTGGAGATTAATGAAA+TGG + chr1_2:78085185-78085204 Msa0094730:intron 35.0%
CAACTAGAGCTTATAAAGCT+TGG + chr1_2:78085400-78085419 Msa0094730:intron 35.0%
TAGGCCCTTCAAATCAAAAT+TGG - chr1_2:78085605-78085624 None:intergenic 35.0%
AAAATAAACAGAGCTTTCCC+AGG - chr1_2:78085726-78085745 None:intergenic 35.0%
AAAAACACACTTCCTCAAAC+TGG + chr1_2:78085750-78085769 Msa0094730:CDS 35.0%
AAGATGCATTTACCAGTTTG+AGG - chr1_2:78085765-78085784 None:intergenic 35.0%
TCAAACGTGCACAAAATCAA+TGG + chr1_2:78085993-78086012 Msa0094730:intron 35.0%
CCACATAAGATGAGAAGATT+TGG - chr1_2:78086110-78086129 None:intergenic 35.0%
CCAAATCTTCTCATCTTATG+TGG + chr1_2:78086107-78086126 Msa0094730:intron 35.0%
TAGCTGTAGATGGAATCAAA+AGG - chr1_2:78086146-78086165 Msa0094740:three_prime_UTR 35.0%
TACAGCAGTGAGAAAACATT+TGG + chr1_2:78086225-78086244 Msa0094730:CDS 35.0%
TTGTTACACTCATAGATTGC+AGG + chr1_2:78086499-78086518 Msa0094730:three_prime_UTR 35.0%
CATTTGCTTTGTAGATGTTG+AGG - chr1_2:78086662-78086681 Msa0094740:CDS 35.0%
AATTTCACCAGTCAATACCT+GGG - chr1_2:78086700-78086719 Msa0094740:CDS 35.0%
TAATTTCACCAGTCAATACC+TGG - chr1_2:78086701-78086720 Msa0094740:CDS 35.0%
GTCTACTTGCAGCTAATTAT+TGG - chr1_2:78086774-78086793 Msa0094740:intron 35.0%
!!! CATTCAGCTGTCATTTTGTA+TGG + chr1_2:78086885-78086904 Msa0094730:intron 35.0%
! ACTTTGTTGTGAAATTGGTC+AGG - chr1_2:78086922-78086941 Msa0094740:intron 35.0%
!!! TGTGCATATATTTTGGAACC+TGG + chr1_2:78087009-78087028 Msa0094730:three_prime_UTR 35.0%
!!! GTGCATATATTTTGGAACCT+GGG + chr1_2:78087010-78087029 Msa0094730:three_prime_UTR 35.0%
!!! TGCATATATTTTGGAACCTG+GGG + chr1_2:78087011-78087030 Msa0094730:three_prime_UTR 35.0%
AAACTGTATCCCAAAGGAAA+AGG - chr1_2:78087096-78087115 Msa0094740:CDS 35.0%
CAGATAAAACTGTATCCCAA+AGG - chr1_2:78087102-78087121 Msa0094740:CDS 35.0%
! ACATGGAGAAGCTTTAGCAT+CGG - chr1_2:78084132-78084151 None:intergenic 40.0%
CTTACACGTTAAATTGACGG+TGG + chr1_2:78084390-78084409 Msa0094730:intron 40.0%
AATAATACGGGAACTCGATC+GGG - chr1_2:78084733-78084752 None:intergenic 40.0%
TCTTCTTGTCCAATTCAGGT+AGG - chr1_2:78084866-78084885 None:intergenic 40.0%
! CAACAAAGCCTTATCACACT+AGG - chr1_2:78085271-78085290 None:intergenic 40.0%
GAGTTAGACTGAAGCTTCTA+AGG + chr1_2:78085464-78085483 Msa0094730:intron 40.0%
TAGTTAAGGTTCTGCCTTCT+TGG + chr1_2:78085818-78085837 Msa0094730:intron 40.0%
AGCATGTTTAGTATCACGGT+GGG + chr1_2:78085919-78085938 Msa0094730:intron 40.0%
! ACGGTGAACTATTGCGATTT+TGG - chr1_2:78085949-78085968 None:intergenic 40.0%
TGACGAGTTAGACAGAATCA+CGG - chr1_2:78085968-78085987 None:intergenic 40.0%
! GTCATAAGACTAGCTGTAGA+TGG - chr1_2:78086156-78086175 Msa0094740:three_prime_UTR 40.0%
CTTGTAAGTTTGGTAGATGG+AGG - chr1_2:78086178-78086197 Msa0094740:three_prime_UTR 40.0%
CTCCATCTACCAAACTTACA+AGG + chr1_2:78086176-78086195 Msa0094730:CDS 40.0%
CTCCTTGTAAGTTTGGTAGA+TGG - chr1_2:78086181-78086200 Msa0094740:three_prime_UTR 40.0%
CATCTACCAAACTTACAAGG+AGG + chr1_2:78086179-78086198 Msa0094730:CDS 40.0%
AAACTTACAAGGAGGAGGAT+GGG + chr1_2:78086187-78086206 Msa0094730:CDS 40.0%
CATAGATTGCAGGATTGACA+AGG + chr1_2:78086509-78086528 Msa0094730:three_prime_UTR 40.0%
GATGTTGAGGTTACAGTTCT+TGG - chr1_2:78086649-78086668 Msa0094740:CDS 40.0%
ATTTCACCAGTCAATACCTG+GGG - chr1_2:78086699-78086718 Msa0094740:CDS 40.0%
ACCTTTCCAAAACTGATGCT+TGG + chr1_2:78086954-78086973 Msa0094730:intron 40.0%
!!! TCCAAGCATCAGTTTTGGAA+AGG - chr1_2:78086958-78086977 Msa0094740:CDS 40.0%
! AGCAATCCAAGCATCAGTTT+TGG - chr1_2:78086963-78086982 Msa0094740:CDS 40.0%
!!! GCATATATTTTGGAACCTGG+GGG + chr1_2:78087012-78087031 Msa0094730:three_prime_UTR 40.0%
!!! CATATATTTTGGAACCTGGG+GGG + chr1_2:78087013-78087032 Msa0094730:three_prime_UTR 40.0%
!! CTGTTGGGTTAGCTAAAGTT+AGG + chr1_2:78087040-78087059 Msa0094730:three_prime_UTR 40.0%
GCGATTGTTCGAGATGAACA+TGG - chr1_2:78084149-78084168 None:intergenic 45.0%
ACTAACTCAGCTTGGTACCT+GGG - chr1_2:78084180-78084199 None:intergenic 45.0%
AACTAACTCAGCTTGGTACC+TGG - chr1_2:78084181-78084200 None:intergenic 45.0%
TAAATTGACGGTGGACGCAA+CGG + chr1_2:78084399-78084418 Msa0094730:intron 45.0%
TAGGACAGACCTACCTGAAT+TGG + chr1_2:78084854-78084873 Msa0094730:CDS 45.0%
TTGGGATGGAAACTCGTATC+AGG + chr1_2:78085037-78085056 Msa0094730:intron 45.0%
!! AGAGTTGATAAGATGGAGGG+TGG + chr1_2:78085105-78085124 Msa0094730:intron 45.0%
GAGGGTGGTTAAATCACTAG+AGG + chr1_2:78085120-78085139 Msa0094730:intron 45.0%
GGTGGTTAAATCACTAGAGG+TGG + chr1_2:78085123-78085142 Msa0094730:intron 45.0%
GGTTAAATCACTAGAGGTGG+AGG + chr1_2:78085126-78085145 Msa0094730:intron 45.0%
ATGGCGTTGCTCAATTCATG+TGG + chr1_2:78085236-78085255 Msa0094730:intron 45.0%
TGACCACACCTAGTGTGATA+AGG + chr1_2:78085260-78085279 Msa0094730:intron 45.0%
! AAGCCTTATCACACTAGGTG+TGG - chr1_2:78085266-78085285 None:intergenic 45.0%
! GCGTGAGACAATCTAAAGCA+TGG - chr1_2:78085341-78085360 None:intergenic 45.0%
ATAAACAGAGCTTTCCCAGG+AGG - chr1_2:78085723-78085742 None:intergenic 45.0%
TAGACGAGTCACATCCAAGA+AGG - chr1_2:78085835-78085854 None:intergenic 45.0%
GAGCATGTTTAGTATCACGG+TGG + chr1_2:78085918-78085937 Msa0094730:intron 45.0%
CTACCAAACTTACAAGGAGG+AGG + chr1_2:78086182-78086201 Msa0094730:CDS 45.0%
CATCCTCCTCCTTGTAAGTT+TGG - chr1_2:78086188-78086207 Msa0094740:three_prime_UTR 45.0%
CAAACTTACAAGGAGGAGGA+TGG + chr1_2:78086186-78086205 Msa0094730:CDS 45.0%
!! TGTTAAGAGTAGCGGACTAG+CGG + chr1_2:78086449-78086468 Msa0094730:three_prime_UTR 45.0%
TGAGGTTACAGTTCTTGGAG+TGG - chr1_2:78086644-78086663 Msa0094740:CDS 45.0%
! ATACCTGGGGTATCTAGTGA+AGG - chr1_2:78086686-78086705 Msa0094740:CDS 45.0%
!! TAGATACCCCAGGTATTGAC+TGG + chr1_2:78086690-78086709 Msa0094730:three_prime_UTR 45.0%
TGTGCCATGTTCATGACTTG+AGG + chr1_2:78086741-78086760 Msa0094730:three_prime_UTR 45.0%
AGTGCCTCAAGTCATGAACA+TGG - chr1_2:78086748-78086767 Msa0094740:CDS 45.0%
!! AAAGGAAAAGGTACCGACTC+GGG - chr1_2:78087084-78087103 Msa0094740:CDS 45.0%
!! CAAAGGAAAAGGTACCGACT+CGG - chr1_2:78087085-78087104 Msa0094740:CDS 45.0%
! GAGTCGGTACCTTTTCCTTT+GGG + chr1_2:78087084-78087103 Msa0094730:three_prime_UTR 45.0%
!! ATAAGATAAATAAATAAAAA+AGG - chr1_2:78084803-78084822 None:intergenic 5.0%
!!! AATTTATAATGTTTTTATTT+AGG + chr1_2:78084943-78084962 Msa0094730:intron 5.0%
!! TTAATAAAATAAAAAAATCA+AGG - chr1_2:78086069-78086088 None:intergenic 5.0%
GCAAGGCAACTAACTCAGCT+TGG - chr1_2:78084188-78084207 None:intergenic 50.0%
GAATCCAGATGAACGACGCA+AGG + chr1_2:78084681-78084700 Msa0094730:intron 50.0%
GATTCCTTGCGTCGTTCATC+TGG - chr1_2:78084688-78084707 None:intergenic 50.0%
GGATGGAAACTCGTATCAGG+TGG + chr1_2:78085040-78085059 Msa0094730:intron 50.0%
TCGTATCAGGTGGTTTGAGC+TGG + chr1_2:78085050-78085069 Msa0094730:intron 50.0%
TTGTCTCACGCCTGAGCATA+AGG + chr1_2:78085350-78085369 Msa0094730:intron 50.0%
TCAACACCCTCCTTATGCTC+AGG - chr1_2:78085363-78085382 None:intergenic 50.0%
GTTCCTCGAGACATGTCTGT+TGG + chr1_2:78085660-78085679 Msa0094730:CDS 50.0%
TGTCCAACAGACATGTCTCG+AGG - chr1_2:78085666-78085685 None:intergenic 50.0%
TGTCCTTCACTAGATACCCC+AGG + chr1_2:78086680-78086699 Msa0094730:three_prime_UTR 50.0%
AGCAAGATATCTGCCCGAGT+CGG + chr1_2:78087068-78087087 Msa0094730:three_prime_UTR 50.0%
! CGAGTCGGTACCTTTTCCTT+TGG + chr1_2:78087083-78087102 Msa0094730:three_prime_UTR 50.0%
AACAATCGCACCCAATGCCC+AGG + chr1_2:78084160-78084179 Msa0094730:five_prime_UTR 55.0%
CAGCTTGGTACCTGGGCATT+GGG - chr1_2:78084173-78084192 None:intergenic 55.0%
TCAGCTTGGTACCTGGGCAT+TGG - chr1_2:78084174-78084193 None:intergenic 55.0%
TCTCACGCCTGAGCATAAGG+AGG + chr1_2:78085353-78085372 Msa0094730:intron 55.0%
CTCACGCCTGAGCATAAGGA+GGG + chr1_2:78085354-78085373 Msa0094730:intron 55.0%
AGTAGGCTACGTTTGCCTCC+TGG + chr1_2:78085705-78085724 Msa0094730:CDS 55.0%
GTAGGCTACGTTTGCCTCCT+GGG + chr1_2:78085706-78085725 Msa0094730:CDS 55.0%
! AACCTGGGGGGATTGCTGTT+GGG + chr1_2:78087025-78087044 Msa0094730:three_prime_UTR 55.0%
!! GAGTAGCGGACTAGCGGACT+TGG + chr1_2:78086455-78086474 Msa0094730:three_prime_UTR 60.0%
GAACCTGGGGGGATTGCTGT+TGG + chr1_2:78087024-78087043 Msa0094730:three_prime_UTR 60.0%
AACCCAACAGCAATCCCCCC+AGG - chr1_2:78087030-78087049 Msa0094740:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 78084119 78087182 78084119 ID=Msa0094730;Name=Msa0094730
chr1_2 mRNA 78084119 78087182 78084119 ID=Msa0094730-mRNA-1;Parent=Msa0094730;Name=Msa0094730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=180|1|1|1|0.66|0.42|7|757|118
chr1_2 exon 78084119 78084181 78084119 ID=Msa0094730-mRNA-1:exon:27732;Parent=Msa0094730-mRNA-1
chr1_2 exon 78084432 78084588 78084432 ID=Msa0094730-mRNA-1:exon:27733;Parent=Msa0094730-mRNA-1
chr1_2 exon 78084689 78084744 78084689 ID=Msa0094730-mRNA-1:exon:27734;Parent=Msa0094730-mRNA-1
chr1_2 exon 78084834 78084886 78084834 ID=Msa0094730-mRNA-1:exon:27735;Parent=Msa0094730-mRNA-1
chr1_2 exon 78085653 78085771 78085653 ID=Msa0094730-mRNA-1:exon:27736;Parent=Msa0094730-mRNA-1
chr1_2 exon 78086163 78086781 78086163 ID=Msa0094730-mRNA-1:exon:27737;Parent=Msa0094730-mRNA-1
chr1_2 exon 78086956 78087182 78086956 ID=Msa0094730-mRNA-1:exon:27738;Parent=Msa0094730-mRNA-1
chr1_2 five_prime_UTR 78084119 78084181 78084119 ID=Msa0094730-mRNA-1:five_prime_utr;Parent=Msa0094730-mRNA-1
chr1_2 five_prime_UTR 78084432 78084548 78084432 ID=Msa0094730-mRNA-1:five_prime_utr;Parent=Msa0094730-mRNA-1
chr1_2 CDS 78084549 78084588 78084549 ID=Msa0094730-mRNA-1:cds;Parent=Msa0094730-mRNA-1
chr1_2 CDS 78084689 78084744 78084689 ID=Msa0094730-mRNA-1:cds;Parent=Msa0094730-mRNA-1
chr1_2 CDS 78084834 78084886 78084834 ID=Msa0094730-mRNA-1:cds;Parent=Msa0094730-mRNA-1
chr1_2 CDS 78085653 78085771 78085653 ID=Msa0094730-mRNA-1:cds;Parent=Msa0094730-mRNA-1
chr1_2 CDS 78086163 78086251 78086163 ID=Msa0094730-mRNA-1:cds;Parent=Msa0094730-mRNA-1
chr1_2 three_prime_UTR 78086252 78086781 78086252 ID=Msa0094730-mRNA-1:three_prime_utr;Parent=Msa0094730-mRNA-1
chr1_2 three_prime_UTR 78086956 78087182 78086956 ID=Msa0094730-mRNA-1:three_prime_utr;Parent=Msa0094730-mRNA-1
Gene Sequence

>Msa0094730

ATGGGAAAGATCAATTCCTTCAAACAAGAATTCTCTCTCGATGAACGACGCAAGGAATCACAAAGCATCATTGTTAAATATCCCGATCGAGTTCCCGTCATCATTGAGAAATATTCTAGGACAGACCTACCTGAATTGGACAAGAAGAAATACTTGGTTCCTCGAGACATGTCTGTTGGACAGTTTATTCACATTTTAAGTAGTAGGCTACGTTTGCCTCCTGGGAAAGCTCTGTTTATTTTTGTGAAAAACACACTTCCTCAAACTGCTAGTCTTATGACCTCCATCTACCAAACTTACAAGGAGGAGGATGGGTTTCTGTATATGTGTTACAGCAGTGAGAAAACATTTGGTTAA
Protein sequence

>Msa0094730

MGKINSFKQEFSLDERRKESQSIIVKYPDRVPVIIEKYSRTDLPELDKKKYLVPRDMSVGQFIHILSSRLRLPPGKALFIFVKNTLPQTASLMTSIYQTYKEEDGFLYMCYSSEKTFG*