AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1257330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257330 MtrunA17_Chr4g0069171 98.305 118 2 0 1 118 1 118 3.61e-83 238
Msa1257330 MtrunA17_Chr7g0260981 97.458 118 3 0 1 118 1 118 2.49e-82 236
Msa1257330 MtrunA17_Chr1g0194141 68.644 118 37 0 1 118 1 118 7.17e-60 179
Msa1257330 MtrunA17_Chr4g0024721 57.895 114 48 0 5 118 4 117 4.48e-48 149
Msa1257330 MtrunA17_Chr2g0322571 56.140 114 50 0 5 118 4 117 2.24e-46 145
Msa1257330 MtrunA17_Chr2g0288531 55.752 113 50 0 6 118 5 117 6.33e-46 144
Msa1257330 MtrunA17_Chr4g0056301 56.250 112 49 0 7 118 6 117 2.13e-45 143
Msa1257330 MtrunA17_Chr4g0019141 49.107 112 55 2 6 115 5 116 9.23e-32 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257330 AT3G06420.1 72.174 115 32 0 4 118 5 119 3.50e-61 183
Msa1257330 AT3G15580.1 70.435 115 34 0 4 118 1 115 1.12e-60 182
Msa1257330 AT2G05630.1 55.652 115 51 0 4 118 3 117 7.13e-48 149
Msa1257330 AT4G04620.3 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa1257330 AT4G04620.2 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa1257330 AT4G04620.1 57.018 114 49 0 5 118 4 117 4.71e-46 145
Msa1257330 AT4G21980.1 57.018 114 49 0 5 118 4 117 4.82e-46 145
Msa1257330 AT1G62040.1 57.018 114 49 0 5 118 4 117 5.84e-46 144
Msa1257330 AT4G21980.2 57.018 114 49 0 5 118 19 132 8.25e-46 145
Msa1257330 AT4G16520.2 54.386 114 52 0 5 118 4 117 6.61e-45 142
Msa1257330 AT4G16520.1 54.386 114 52 0 5 118 4 117 6.61e-45 142
Msa1257330 AT2G05630.2 55.556 108 48 0 11 118 54 161 6.46e-44 141
Msa1257330 AT3G60640.1 53.097 113 53 0 6 118 5 117 1.54e-42 136
Msa1257330 AT2G45170.2 50.847 118 58 0 1 118 1 118 3.31e-42 135
Msa1257330 AT2G45170.1 50.847 118 58 0 1 118 1 118 3.31e-42 135
Msa1257330 AT4G16520.3 52.273 88 42 0 31 118 30 117 5.78e-32 109
Msa1257330 AT1G62040.2 53.846 91 42 0 5 95 4 94 6.25e-31 105

Find 68 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTCTGTGCATATATTT+TGG 0.140548 8_2:+57978922 Msa1257330:three_prime_UTR
CAACAAATCGTTGTTTATAT+TGG 0.212724 8_2:-57978295 Msa1257340:three_prime_UTR
GTCTACTTGCAGCTAATTAT+TGG 0.237931 8_2:-57978691 Msa1257340:intron
TATGAGTGTAACAATCTAAT+TGG 0.265742 8_2:-57978410 Msa1257340:three_prime_UTR
GATGTTGAGGTTACAGTTCT+TGG 0.266368 8_2:-57978566 Msa1257340:CDS
TACTTCTTCTTGTCCAATTC+AGG 0.278998 8_2:-57976783 None:intergenic
CATCCTCCTCCTTGTAAGTT+TGG 0.322568 8_2:-57978105 Msa1257340:three_prime_UTR
AATTGAAGTTGTAATGAAAA+TGG 0.327561 8_2:-57976369 None:intergenic
AGCAATCCAAGCATCAGTTT+TGG 0.342021 8_2:-57978880 Msa1257340:CDS
CAGCTTGGTACCTGGGCATT+GGG 0.361123 8_2:-57976093 None:intergenic
TAGGACAGACCTACCTGAAT+TGG 0.369195 8_2:+57976770 Msa1257330:CDS
CGTTGTTTATATTGGAGATT+CGG 0.378593 8_2:-57978287 Msa1257340:three_prime_UTR
GAGAGAGAATTCTTGTTTGA+AGG 0.384970 8_2:-57976481 None:intergenic
CTGTTGGGTTAGCTAAAGTT+AGG 0.387750 8_2:+57978960 Msa1257330:three_prime_UTR
ATTACTGCACAAAATAAATC+TGG 0.389938 8_2:+57978637 Msa1257330:three_prime_UTR
GAGTATTGTTTGAACTCAAC+AGG 0.391562 8_2:-57979092 Msa1257340:intron
CTCCTTGTAAGTTTGGTAGA+TGG 0.391844 8_2:-57978098 Msa1257340:three_prime_UTR
TCAGCTTGGTACCTGGGCAT+TGG 0.400093 8_2:-57976094 None:intergenic
CAAATCAATATCATATATCA+TGG 0.403889 8_2:+57976446 Msa1257330:exon
TTGTTACACTCATAGATTGC+AGG 0.409345 8_2:+57978419 Msa1257330:three_prime_UTR
AATAATACGGGAACTCGATC+GGG 0.413583 8_2:-57976646 None:intergenic
TACAGCAGTGAGAAAACATT+TGG 0.420898 8_2:+57978145 Msa1257330:CDS
TCATCATTGAGAAATATTCT+AGG 0.422511 8_2:+57976751 Msa1257330:CDS
CACAATTGACAAATACTTGA+AGG 0.442560 8_2:-57978205 Msa1257340:three_prime_UTR
ACCTTTCCAAAACTGATGCT+TGG 0.443667 8_2:+57978874 Msa1257330:intron
AGTAGGCTACGTTTGCCTCC+TGG 0.446454 8_2:+57977625 Msa1257330:CDS
AAGATGCATTTACCAGTTTG+AGG 0.454421 8_2:-57977682 None:intergenic
GTAGGCTACGTTTGCCTCCT+GGG 0.455739 8_2:+57977626 Msa1257330:CDS
GAGTAGCGGACTAGCGGACT+TGG 0.457546 8_2:+57978375 Msa1257330:three_prime_UTR
AAATAATACGGGAACTCGAT+CGG 0.458795 8_2:-57976647 None:intergenic
TAATTTCACCAGTCAATACC+TGG 0.466177 8_2:-57978618 Msa1257340:CDS
GAGAAAACATTTGGTTAACT+TGG 0.487241 8_2:+57978154 Msa1257330:exon
ATACAAGAATGTAGTTGCAT+TGG 0.491129 8_2:-57978502 Msa1257340:three_prime_UTR
AAACTGTATCCCAAAGGAAA+AGG 0.507112 8_2:-57979013 Msa1257340:CDS
CTCCATCTACCAAACTTACA+AGG 0.518610 8_2:+57978096 Msa1257330:CDS
GTAATGAAAATGGAAGGAAT+TGG 0.521825 8_2:-57976359 None:intergenic
CAAACTTACAAGGAGGAGGA+TGG 0.523873 8_2:+57978106 Msa1257330:CDS
TTCAAACAAGAATTCTCTCT+CGG 0.525359 8_2:+57976483 Msa1257330:CDS
GTTCCTCGAGACATGTCTGT+TGG 0.526742 8_2:+57977580 Msa1257330:CDS
CATTTGCTTTGTAGATGTTG+AGG 0.531213 8_2:-57978579 Msa1257340:CDS
CAAAGGAAAAGGTACTGACT+CGG 0.538845 8_2:-57979002 Msa1257340:CDS
AACAATCGCACCCAATGCCC+AGG 0.547604 8_2:+57976083 Msa1257330:five_prime_UTR
TCTTCTTGTCCAATTCAGGT+AGG 0.548533 8_2:-57976779 None:intergenic
TAGATACCCCAGGTATTGAC+TGG 0.568960 8_2:+57978610 Msa1257330:three_prime_UTR
TATTGATTTGAATTATGTGT+TGG 0.578463 8_2:-57976433 None:intergenic
GAAGTTGTAATGAAAATGGA+AGG 0.580623 8_2:-57976365 None:intergenic
GCGATTGTTCGAGATGAACA+TGG 0.580865 8_2:-57976069 None:intergenic
AAATCAATATCATATATCAT+GGG 0.582392 8_2:+57976447 Msa1257330:exon
AAAGGAAAAGGTACTGACTC+GGG 0.585829 8_2:-57979001 Msa1257340:CDS
AAAATAAACAGAGCTTTCCC+AGG 0.586278 8_2:-57977643 None:intergenic
CTACCAAACTTACAAGGAGG+AGG 0.590671 8_2:+57978102 Msa1257330:CDS
CTTGTAAGTTTGGTAGATGG+AGG 0.594122 8_2:-57978095 Msa1257340:three_prime_UTR
GAATCCAGATGAACGACGCA+AGG 0.597013 8_2:+57976597 Msa1257330:intron
AAACTTACAAGGAGGAGGAT+GGG 0.597052 8_2:+57978107 Msa1257330:CDS
AATTTCACCAGTCAATACCT+GGG 0.612530 8_2:-57978617 Msa1257340:CDS
CATAGATTGCAGGATTGACA+AGG 0.619050 8_2:+57978429 Msa1257330:three_prime_UTR
TGAGGTTACAGTTCTTGGAG+TGG 0.629632 8_2:-57978561 Msa1257340:CDS
ATACCTGGGGTATCTAGTGA+AGG 0.632705 8_2:-57978603 Msa1257340:CDS
CAGATAAAACTGTATCCCAA+AGG 0.633902 8_2:-57979019 Msa1257340:CDS
TGTCCTTCACTAGATACCCC+AGG 0.633913 8_2:+57978600 Msa1257330:three_prime_UTR
TGTTAAGAGTAGCGGACTAG+CGG 0.641760 8_2:+57978369 Msa1257330:three_prime_UTR
ACATGGAGAAGCTTTAGCAT+CGG 0.648150 8_2:-57976052 None:intergenic
TGTGCCATGTTCATGACTTG+AGG 0.660953 8_2:+57978661 Msa1257330:three_prime_UTR
ATAAACAGAGCTTTCCCAGG+AGG 0.685967 8_2:-57977640 None:intergenic
AGTGCCTCAAGTCATGAACA+TGG 0.700230 8_2:-57978665 Msa1257340:CDS
TGTCCAACAGACATGTCTCG+AGG 0.712779 8_2:-57977583 None:intergenic
ATTTCACCAGTCAATACCTG+GGG 0.717112 8_2:-57978616 Msa1257340:CDS
CATCTACCAAACTTACAAGG+AGG 0.745667 8_2:+57978099 Msa1257330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTAATTAACTATTTTTG+TGG + chr8_2:57976249-57976268 Msa1257330:intron 10.0%
!!! ATATATTATAGAATTCTTTT+TGG + chr8_2:57977407-57977426 Msa1257330:intron 10.0%
!! AAATCAATATCATATATCAT+GGG + chr8_2:57976447-57976466 Msa1257330:exon 15.0%
!!! TATTTTATATTGAAATGTGA+GGG - chr8_2:57976535-57976554 None:intergenic 15.0%
!!! TTATTTTATATTGAAATGTG+AGG - chr8_2:57976536-57976555 None:intergenic 15.0%
!! ATTGATTGAGAAAAAAAAAA+TGG - chr8_2:57976680-57976699 None:intergenic 15.0%
!! ATTTATCTTATTCTTATTGA+AGG + chr8_2:57976728-57976747 Msa1257330:intron 15.0%
!!! ACAAAATTGACAAATTTTAT+AGG - chr8_2:57976928-57976947 None:intergenic 15.0%
!! TCTTAACATGAAAAAAAATA+TGG - chr8_2:57978357-57978376 Msa1257340:three_prime_UTR 15.0%
!! AATTGAAGTTGTAATGAAAA+TGG - chr8_2:57976372-57976391 None:intergenic 20.0%
!!! TATTGATTTGAATTATGTGT+TGG - chr8_2:57976436-57976455 None:intergenic 20.0%
!! CAAATCAATATCATATATCA+TGG + chr8_2:57976446-57976465 Msa1257330:exon 20.0%
!!! ATGTTTTCATTTTGCATTTT+TGG + chr8_2:57976805-57976824 Msa1257330:intron 20.0%
!!! AATGATGATGTTGAATAATT+AGG - chr8_2:57976837-57976856 None:intergenic 20.0%
!!! AAATTCAAAATCAGCTTTTA+AGG - chr8_2:57977352-57977371 None:intergenic 20.0%
!!! AATACCAATTTTGATTTGAA+GGG + chr8_2:57977518-57977537 Msa1257330:intron 20.0%
!! AGAAAACAAAATATGAGATT+AGG - chr8_2:57977544-57977563 None:intergenic 20.0%
!!! TTATTCACATTTTAAGTAGT+AGG + chr8_2:57977608-57977627 Msa1257330:CDS 20.0%
!! GTTATAGTAGTTATTAGTTA+AGG + chr8_2:57977724-57977743 Msa1257330:intron 20.0%
!! CAAAAAGTTATCATATAACT+TGG + chr8_2:57979046-57979065 Msa1257330:three_prime_UTR 20.0%
! AATGGAGGAGAAAAATAATA+CGG - chr8_2:57976662-57976681 None:intergenic 25.0%
! GATTGAGAAAAAAAAAATGG+AGG - chr8_2:57976677-57976696 None:intergenic 25.0%
! TCATCATTGAGAAATATTCT+AGG + chr8_2:57976751-57976770 Msa1257330:CDS 25.0%
!! TTTGTCAATTTTGTATGTGT+TGG + chr8_2:57976934-57976953 Msa1257330:intron 25.0%
!! TTGTCAATTTTGTATGTGTT+GGG + chr8_2:57976935-57976954 Msa1257330:intron 25.0%
! TAAAGCATGGAAAATACAAA+TGG - chr8_2:57977244-57977263 None:intergenic 25.0%
!! TTTATAAGCTCTAGTTGATT+AGG - chr8_2:57977313-57977332 None:intergenic 25.0%
!!! AAGCTGATTTTGAATTTATG+TGG + chr8_2:57977355-57977374 Msa1257330:intron 25.0%
!!! GAATACCAATTTTGATTTGA+AGG + chr8_2:57977517-57977536 Msa1257330:intron 25.0%
!! TTTTCTCTCTTTTAGATACT+TGG + chr8_2:57977558-57977577 Msa1257330:intron 25.0%
! TGCAAATGCATAATAAATCA+TGG + chr8_2:57977947-57977966 Msa1257330:intron 25.0%
! GCAAATGCATAATAAATCAT+GGG + chr8_2:57977948-57977967 Msa1257330:intron 25.0%
! CAAATGCATAATAAATCATG+GGG + chr8_2:57977949-57977968 Msa1257330:intron 25.0%
! AAATGCATAATAAATCATGG+GGG + chr8_2:57977950-57977969 Msa1257330:intron 25.0%
! CAACAAATCGTTGTTTATAT+TGG - chr8_2:57978298-57978317 Msa1257340:three_prime_UTR 25.0%
!!! TTTTTTCATGTTAAGAGTAG+CGG + chr8_2:57978361-57978380 Msa1257330:three_prime_UTR 25.0%
! TATGAGTGTAACAATCTAAT+TGG - chr8_2:57978413-57978432 Msa1257340:three_prime_UTR 25.0%
! ATTACTGCACAAAATAAATC+TGG + chr8_2:57978637-57978656 Msa1257330:three_prime_UTR 25.0%
!! TATTGTCTGTGCATATATTT+TGG + chr8_2:57978922-57978941 Msa1257330:three_prime_UTR 25.0%
!! GTTATATGATAACTTTTTGC+AGG - chr8_2:57979045-57979064 Msa1257340:intron 25.0%
AATAGAACAAATAGCATGCA+AGG - chr8_2:57976128-57976147 None:intergenic 30.0%
AAGTCAATGACAATCAACAA+CGG - chr8_2:57976289-57976308 None:intergenic 30.0%
TGACTTACACGTTAAATTGA+CGG + chr8_2:57976303-57976322 Msa1257330:intron 30.0%
GTAATGAAAATGGAAGGAAT+TGG - chr8_2:57976362-57976381 None:intergenic 30.0%
GAAGTTGTAATGAAAATGGA+AGG - chr8_2:57976368-57976387 None:intergenic 30.0%
TTCAAACAAGAATTCTCTCT+CGG + chr8_2:57976483-57976502 Msa1257330:CDS 30.0%
!!! GGATTTTGAGATGAAAACAA+AGG - chr8_2:57976514-57976533 None:intergenic 30.0%
! ATTGCATTAATGTTTGCTGA+TGG + chr8_2:57976565-57976584 Msa1257330:intron 30.0%
ATGGAGGAGAAAAATAATAC+GGG - chr8_2:57976661-57976680 None:intergenic 30.0%
!! AGTAATGAGAGTTGATAAGA+TGG + chr8_2:57977014-57977033 Msa1257330:intron 30.0%
AAGACTGAGAAAAGCTATAA+GGG + chr8_2:57977065-57977084 Msa1257330:intron 30.0%
TGATGCATGATAGAATGTTA+TGG + chr8_2:57977133-57977152 Msa1257330:intron 30.0%
AACTAGAGCTTATAAAGCTT+GGG + chr8_2:57977317-57977336 Msa1257330:intron 30.0%
TAGACTGAAGCTAAATTCTA+AGG + chr8_2:57977384-57977403 Msa1257330:intron 30.0%
!! CTTTTTGGTGATAGCAATAT+AGG + chr8_2:57977422-57977441 Msa1257330:intron 30.0%
TTAGAGCATGTTTAGTATCA+CGG + chr8_2:57977835-57977854 Msa1257330:intron 30.0%
GAGAAAACATTTGGTTAACT+TGG + chr8_2:57978154-57978173 Msa1257330:exon 30.0%
CACAATTGACAAATACTTGA+AGG - chr8_2:57978208-57978227 Msa1257340:three_prime_UTR 30.0%
CGTTGTTTATATTGGAGATT+CGG - chr8_2:57978290-57978309 Msa1257340:three_prime_UTR 30.0%
ATACAAGAATGTAGTTGCAT+TGG - chr8_2:57978505-57978524 Msa1257340:three_prime_UTR 30.0%
! GTTACACTTTGTTGTGAAAT+TGG - chr8_2:57978847-57978866 Msa1257340:intron 30.0%
! GAGAGAGAATTCTTGTTTGA+AGG - chr8_2:57976484-57976503 None:intergenic 35.0%
AAATAATACGGGAACTCGAT+CGG - chr8_2:57976650-57976669 None:intergenic 35.0%
TACTTCTTCTTGTCCAATTC+AGG - chr8_2:57976786-57976805 None:intergenic 35.0%
! CAATTTTGTATGTGTTGGGA+TGG + chr8_2:57976939-57976958 Msa1257330:intron 35.0%
!! AATGAGAGTTGATAAGATGG+AGG + chr8_2:57977017-57977036 Msa1257330:intron 35.0%
!! ATGAGAGTTGATAAGATGGA+GGG + chr8_2:57977018-57977037 Msa1257330:intron 35.0%
GAAGACTGAGAAAAGCTATA+AGG + chr8_2:57977064-57977083 Msa1257330:intron 35.0%
GGAAACTATTAAGAAGAGTG+TGG + chr8_2:57977086-57977105 Msa1257330:intron 35.0%
GAGTGTGGAGATTAATGAAA+TGG + chr8_2:57977101-57977120 Msa1257330:intron 35.0%
CAACTAGAGCTTATAAAGCT+TGG + chr8_2:57977316-57977335 Msa1257330:intron 35.0%
TAGGCCCTTCAAATCAAAAT+TGG - chr8_2:57977525-57977544 None:intergenic 35.0%
AAAATAAACAGAGCTTTCCC+AGG - chr8_2:57977646-57977665 None:intergenic 35.0%
AAAAACACACTTCCTCAAAC+TGG + chr8_2:57977670-57977689 Msa1257330:CDS 35.0%
AAGATGCATTTACCAGTTTG+AGG - chr8_2:57977685-57977704 None:intergenic 35.0%
TCAAACGTGCACAAAATCAA+TGG + chr8_2:57977913-57977932 Msa1257330:intron 35.0%
CCACATAAGATGAGAAGATT+TGG - chr8_2:57978030-57978049 None:intergenic 35.0%
CCAAATCTTCTCATCTTATG+TGG + chr8_2:57978027-57978046 Msa1257330:intron 35.0%
TAGCTGTAGATGGAATCAAA+AGG - chr8_2:57978066-57978085 Msa1257340:three_prime_UTR 35.0%
TACAGCAGTGAGAAAACATT+TGG + chr8_2:57978145-57978164 Msa1257330:CDS 35.0%
TTGTTACACTCATAGATTGC+AGG + chr8_2:57978419-57978438 Msa1257330:three_prime_UTR 35.0%
CATTTGCTTTGTAGATGTTG+AGG - chr8_2:57978582-57978601 Msa1257340:CDS 35.0%
AATTTCACCAGTCAATACCT+GGG - chr8_2:57978620-57978639 Msa1257340:CDS 35.0%
TAATTTCACCAGTCAATACC+TGG - chr8_2:57978621-57978640 Msa1257340:CDS 35.0%
GTCTACTTGCAGCTAATTAT+TGG - chr8_2:57978694-57978713 Msa1257340:intron 35.0%
!!! CATTCAGCTGTCATTTTGTA+TGG + chr8_2:57978805-57978824 Msa1257330:intron 35.0%
! ACTTTGTTGTGAAATTGGTC+AGG - chr8_2:57978842-57978861 Msa1257340:intron 35.0%
!!! TGTGCATATATTTTGGAACC+TGG + chr8_2:57978929-57978948 Msa1257330:three_prime_UTR 35.0%
!!! GTGCATATATTTTGGAACCT+GGG + chr8_2:57978930-57978949 Msa1257330:three_prime_UTR 35.0%
!!! TGCATATATTTTGGAACCTG+GGG + chr8_2:57978931-57978950 Msa1257330:three_prime_UTR 35.0%
AAACTGTATCCCAAAGGAAA+AGG - chr8_2:57979016-57979035 Msa1257340:CDS 35.0%
CAGATAAAACTGTATCCCAA+AGG - chr8_2:57979022-57979041 Msa1257340:CDS 35.0%
! ACATGGAGAAGCTTTAGCAT+CGG - chr8_2:57976055-57976074 None:intergenic 40.0%
CTTACACGTTAAATTGACGG+TGG + chr8_2:57976306-57976325 Msa1257330:intron 40.0%
AATAATACGGGAACTCGATC+GGG - chr8_2:57976649-57976668 None:intergenic 40.0%
TCTTCTTGTCCAATTCAGGT+AGG - chr8_2:57976782-57976801 None:intergenic 40.0%
! CAACAAAGCCTTATCACACT+AGG - chr8_2:57977187-57977206 None:intergenic 40.0%
TAGTTAAGGTTCTGCCTTCT+TGG + chr8_2:57977738-57977757 Msa1257330:intron 40.0%
AGCATGTTTAGTATCACGGT+GGG + chr8_2:57977839-57977858 Msa1257330:intron 40.0%
! ACGGTGAACTATTGCGATTT+TGG - chr8_2:57977869-57977888 None:intergenic 40.0%
TGACGAGTTAGACAGAATCA+CGG - chr8_2:57977888-57977907 None:intergenic 40.0%
! GTCATAAGACTAGCTGTAGA+TGG - chr8_2:57978076-57978095 Msa1257340:three_prime_UTR 40.0%
CTTGTAAGTTTGGTAGATGG+AGG - chr8_2:57978098-57978117 Msa1257340:three_prime_UTR 40.0%
CTCCATCTACCAAACTTACA+AGG + chr8_2:57978096-57978115 Msa1257330:CDS 40.0%
CTCCTTGTAAGTTTGGTAGA+TGG - chr8_2:57978101-57978120 Msa1257340:three_prime_UTR 40.0%
CATCTACCAAACTTACAAGG+AGG + chr8_2:57978099-57978118 Msa1257330:CDS 40.0%
AAACTTACAAGGAGGAGGAT+GGG + chr8_2:57978107-57978126 Msa1257330:CDS 40.0%
CATAGATTGCAGGATTGACA+AGG + chr8_2:57978429-57978448 Msa1257330:three_prime_UTR 40.0%
GATGTTGAGGTTACAGTTCT+TGG - chr8_2:57978569-57978588 Msa1257340:CDS 40.0%
ATTTCACCAGTCAATACCTG+GGG - chr8_2:57978619-57978638 Msa1257340:CDS 40.0%
ACCTTTCCAAAACTGATGCT+TGG + chr8_2:57978874-57978893 Msa1257330:intron 40.0%
!!! TCCAAGCATCAGTTTTGGAA+AGG - chr8_2:57978878-57978897 Msa1257340:CDS 40.0%
! AGCAATCCAAGCATCAGTTT+TGG - chr8_2:57978883-57978902 Msa1257340:CDS 40.0%
!!! GCATATATTTTGGAACCTGG+GGG + chr8_2:57978932-57978951 Msa1257330:three_prime_UTR 40.0%
!!! CATATATTTTGGAACCTGGG+GGG + chr8_2:57978933-57978952 Msa1257330:three_prime_UTR 40.0%
!! CTGTTGGGTTAGCTAAAGTT+AGG + chr8_2:57978960-57978979 Msa1257330:three_prime_UTR 40.0%
!! AAAGGAAAAGGTACTGACTC+GGG - chr8_2:57979004-57979023 Msa1257340:CDS 40.0%
!! CAAAGGAAAAGGTACTGACT+CGG - chr8_2:57979005-57979024 Msa1257340:CDS 40.0%
! GAGTCAGTACCTTTTCCTTT+GGG + chr8_2:57979004-57979023 Msa1257330:three_prime_UTR 40.0%
GCGATTGTTCGAGATGAACA+TGG - chr8_2:57976072-57976091 None:intergenic 45.0%
ACTAACTCAGCTTGGTACCT+GGG - chr8_2:57976103-57976122 None:intergenic 45.0%
AACTAACTCAGCTTGGTACC+TGG - chr8_2:57976104-57976123 None:intergenic 45.0%
TAAATTGACGGTGGACGCAA+CGG + chr8_2:57976315-57976334 Msa1257330:intron 45.0%
TAGGACAGACCTACCTGAAT+TGG + chr8_2:57976770-57976789 Msa1257330:CDS 45.0%
TTGGGATGGAAACTCGTATC+AGG + chr8_2:57976953-57976972 Msa1257330:intron 45.0%
!! AGAGTTGATAAGATGGAGGG+TGG + chr8_2:57977021-57977040 Msa1257330:intron 45.0%
GAGGGTGGTTAAATCACTAG+AGG + chr8_2:57977036-57977055 Msa1257330:intron 45.0%
GGTGGTTAAATCACTAGAGG+TGG + chr8_2:57977039-57977058 Msa1257330:intron 45.0%
GGTTAAATCACTAGAGGTGG+AGG + chr8_2:57977042-57977061 Msa1257330:intron 45.0%
ATGGCGTTGCTCAATTCATG+TGG + chr8_2:57977152-57977171 Msa1257330:intron 45.0%
TGACCACACCTAGTGTGATA+AGG + chr8_2:57977176-57977195 Msa1257330:intron 45.0%
! AAGCCTTATCACACTAGGTG+TGG - chr8_2:57977182-57977201 None:intergenic 45.0%
! GCGTGAGACAATCTAAAGCA+TGG - chr8_2:57977257-57977276 None:intergenic 45.0%
ATAAACAGAGCTTTCCCAGG+AGG - chr8_2:57977643-57977662 None:intergenic 45.0%
TAGACGAGTCACATCCAAGA+AGG - chr8_2:57977755-57977774 None:intergenic 45.0%
GAGCATGTTTAGTATCACGG+TGG + chr8_2:57977838-57977857 Msa1257330:intron 45.0%
CTACCAAACTTACAAGGAGG+AGG + chr8_2:57978102-57978121 Msa1257330:CDS 45.0%
CATCCTCCTCCTTGTAAGTT+TGG - chr8_2:57978108-57978127 Msa1257340:three_prime_UTR 45.0%
CAAACTTACAAGGAGGAGGA+TGG + chr8_2:57978106-57978125 Msa1257330:CDS 45.0%
!! TGTTAAGAGTAGCGGACTAG+CGG + chr8_2:57978369-57978388 Msa1257330:three_prime_UTR 45.0%
TGAGGTTACAGTTCTTGGAG+TGG - chr8_2:57978564-57978583 Msa1257340:CDS 45.0%
! ATACCTGGGGTATCTAGTGA+AGG - chr8_2:57978606-57978625 Msa1257340:CDS 45.0%
!! TAGATACCCCAGGTATTGAC+TGG + chr8_2:57978610-57978629 Msa1257330:three_prime_UTR 45.0%
TGTGCCATGTTCATGACTTG+AGG + chr8_2:57978661-57978680 Msa1257330:three_prime_UTR 45.0%
AGTGCCTCAAGTCATGAACA+TGG - chr8_2:57978668-57978687 Msa1257340:CDS 45.0%
! CGAGTCAGTACCTTTTCCTT+TGG + chr8_2:57979003-57979022 Msa1257330:three_prime_UTR 45.0%
!! ATAAGATAAATAAATAAAAA+AGG - chr8_2:57976719-57976738 None:intergenic 5.0%
!!! AATTTATAATGTTTTTATTT+AGG + chr8_2:57976859-57976878 Msa1257330:intron 5.0%
!! TTAATAAAATAAAAAAATCA+AGG - chr8_2:57977989-57978008 None:intergenic 5.0%
GCAAGGCAACTAACTCAGCT+TGG - chr8_2:57976111-57976130 None:intergenic 50.0%
GAATCCAGATGAACGACGCA+AGG + chr8_2:57976597-57976616 Msa1257330:intron 50.0%
GATTCCTTGCGTCGTTCATC+TGG - chr8_2:57976604-57976623 None:intergenic 50.0%
GGATGGAAACTCGTATCAGG+TGG + chr8_2:57976956-57976975 Msa1257330:intron 50.0%
TCGTATCAGGTGGTTTGAGC+TGG + chr8_2:57976966-57976985 Msa1257330:intron 50.0%
TTGTCTCACGCCTGAGCATA+AGG + chr8_2:57977266-57977285 Msa1257330:intron 50.0%
TCAACACCCTCCTTATGCTC+AGG - chr8_2:57977279-57977298 None:intergenic 50.0%
GTTCCTCGAGACATGTCTGT+TGG + chr8_2:57977580-57977599 Msa1257330:CDS 50.0%
TGTCCAACAGACATGTCTCG+AGG - chr8_2:57977586-57977605 None:intergenic 50.0%
TGTCCTTCACTAGATACCCC+AGG + chr8_2:57978600-57978619 Msa1257330:three_prime_UTR 50.0%
AACAATCGCACCCAATGCCC+AGG + chr8_2:57976083-57976102 Msa1257330:five_prime_UTR 55.0%
CAGCTTGGTACCTGGGCATT+GGG - chr8_2:57976096-57976115 None:intergenic 55.0%
TCAGCTTGGTACCTGGGCAT+TGG - chr8_2:57976097-57976116 None:intergenic 55.0%
TCTCACGCCTGAGCATAAGG+AGG + chr8_2:57977269-57977288 Msa1257330:intron 55.0%
CTCACGCCTGAGCATAAGGA+GGG + chr8_2:57977270-57977289 Msa1257330:intron 55.0%
AGTAGGCTACGTTTGCCTCC+TGG + chr8_2:57977625-57977644 Msa1257330:CDS 55.0%
GTAGGCTACGTTTGCCTCCT+GGG + chr8_2:57977626-57977645 Msa1257330:CDS 55.0%
! AACCTGGGGGGATTGCTGTT+GGG + chr8_2:57978945-57978964 Msa1257330:three_prime_UTR 55.0%
!! GAGTAGCGGACTAGCGGACT+TGG + chr8_2:57978375-57978394 Msa1257330:three_prime_UTR 60.0%
GAACCTGGGGGGATTGCTGT+TGG + chr8_2:57978944-57978963 Msa1257330:three_prime_UTR 60.0%
AACCCAACAGCAATCCCCCC+AGG - chr8_2:57978950-57978969 Msa1257340:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 57976042 57979102 57976042 ID=Msa1257330;Name=Msa1257330
chr8_2 mRNA 57976042 57979102 57976042 ID=Msa1257330-mRNA-1;Parent=Msa1257330;Name=Msa1257330-mRNA-1;_AED=0.05;_eAED=0.05;_QI=180|1|1|1|0.66|0.42|7|757|118
chr8_2 exon 57976042 57976104 57976042 ID=Msa1257330-mRNA-1:exon:17851;Parent=Msa1257330-mRNA-1
chr8_2 exon 57976348 57976504 57976348 ID=Msa1257330-mRNA-1:exon:17852;Parent=Msa1257330-mRNA-1
chr8_2 exon 57976605 57976660 57976605 ID=Msa1257330-mRNA-1:exon:17853;Parent=Msa1257330-mRNA-1
chr8_2 exon 57976750 57976802 57976750 ID=Msa1257330-mRNA-1:exon:17854;Parent=Msa1257330-mRNA-1
chr8_2 exon 57977573 57977691 57977573 ID=Msa1257330-mRNA-1:exon:17855;Parent=Msa1257330-mRNA-1
chr8_2 exon 57978083 57978701 57978083 ID=Msa1257330-mRNA-1:exon:17856;Parent=Msa1257330-mRNA-1
chr8_2 exon 57978876 57979102 57978876 ID=Msa1257330-mRNA-1:exon:17857;Parent=Msa1257330-mRNA-1
chr8_2 five_prime_UTR 57976042 57976104 57976042 ID=Msa1257330-mRNA-1:five_prime_utr;Parent=Msa1257330-mRNA-1
chr8_2 five_prime_UTR 57976348 57976464 57976348 ID=Msa1257330-mRNA-1:five_prime_utr;Parent=Msa1257330-mRNA-1
chr8_2 CDS 57976465 57976504 57976465 ID=Msa1257330-mRNA-1:cds;Parent=Msa1257330-mRNA-1
chr8_2 CDS 57976605 57976660 57976605 ID=Msa1257330-mRNA-1:cds;Parent=Msa1257330-mRNA-1
chr8_2 CDS 57976750 57976802 57976750 ID=Msa1257330-mRNA-1:cds;Parent=Msa1257330-mRNA-1
chr8_2 CDS 57977573 57977691 57977573 ID=Msa1257330-mRNA-1:cds;Parent=Msa1257330-mRNA-1
chr8_2 CDS 57978083 57978171 57978083 ID=Msa1257330-mRNA-1:cds;Parent=Msa1257330-mRNA-1
chr8_2 three_prime_UTR 57978172 57978701 57978172 ID=Msa1257330-mRNA-1:three_prime_utr;Parent=Msa1257330-mRNA-1
chr8_2 three_prime_UTR 57978876 57979102 57978876 ID=Msa1257330-mRNA-1:three_prime_utr;Parent=Msa1257330-mRNA-1
Gene Sequence

>Msa1257330

ATGGGAAAGATCAATTCCTTCAAACAAGAATTCTCTCTCGATGAACGACGCAAGGAATCACAAAGCATCATTGTTAAATATCCCGATCGAGTTCCCGTCATCATTGAGAAATATTCTAGGACAGACCTACCTGAATTGGACAAGAAGAAATACTTGGTTCCTCGAGACATGTCTGTTGGACAGTTTATTCACATTTTAAGTAGTAGGCTACGTTTGCCTCCTGGGAAAGCTCTGTTTATTTTTGTGAAAAACACACTTCCTCAAACTGCTAGTCTTATGACCTCCATCTACCAAACTTACAAGGAGGAGGATGGGTTTCTGTATATGTGTTACAGCAGTGAGAAAACATTTGGTTAA
Protein sequence

>Msa1257330

MGKINSFKQEFSLDERRKESQSIIVKYPDRVPVIIEKYSRTDLPELDKKKYLVPRDMSVGQFIHILSSRLRLPPGKALFIFVKNTLPQTASLMTSIYQTYKEEDGFLYMCYSSEKTFG*