AlfalfaGEDB | Msa0099920

M. sativa cultivar zhongmu-4 / Msa0099920

KEGG: K03715
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006643 GO:0006664 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0008378 GO:0008610 GO:0009058 GO:0009247 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009668 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010027 GO:0010154 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0035250 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046509 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061024 GO:0061458 GO:0071704 GO:0071840 GO:1901135 GO:1901137 GO:1901576 GO:1903509
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099920	sp\|Q9FZL4\|MGDG_SOYBN	48.000	100	44	3	8	106	5	97	1.93e-19	86.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099920	A0A396JL64	98.077	104	2	0	1	104	1	104	4.92e-66	218

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027390	Msa0099920	0.811089	8.335972e-51	-8.615850e-47
Msa0099920	Msa0165300	0.818115	2.319012e-52	-8.615850e-47
Msa0099920	Msa0959010	0.804031	2.625772e-49	-8.615850e-47
Msa0099920	Msa1219520	0.803613	3.206935e-49	-8.615850e-47
Msa0099920	Msa1409350	0.803014	4.266844e-49	-8.615850e-47
Msa0099920	Msa1422840	0.802093	6.605760e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099920	MtrunA17_Chr1g0150361	98.077	104	2	0	1	104	1	104	9.47e-70	218
Msa0099920	MtrunA17_Chr8g0347051	97.115	104	3	0	1	104	1	104	2.91e-68	214

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 37 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAATGGTGTTGGTGGAAA+TGG	0.257208	1_3:+6301711	Msa0099920:CDS
GGTGGTGGTGTTGGTGGTGA+GGG	0.289092	1_3:+6301690	Msa0099920:CDS
TGGTGGTGGTGTTGGTGGTG+AGG	0.295208	1_3:+6301689	Msa0099920:CDS
TCCGATTGGATTCGCTTCGC+TGG	0.325021	1_3:+6301650	Msa0099920:CDS
GTGAACATGCTTCTGAAGTT+AGG	0.332228	1_3:-6301461	None:intergenic
GTGGTGGTGTTGGTGGTGAG+GGG	0.391464	1_3:+6301691	Msa0099920:CDS
GGTGGTGAGGGGAATGGTGT+TGG	0.397626	1_3:+6301702	Msa0099920:CDS
GGTGTTGGTGGTGAGGGGAA+TGG	0.402477	1_3:+6301696	Msa0099920:CDS
GTTGATGACGGTGGTGGTGG+TGG	0.406284	1_3:+6301675	Msa0099920:CDS
TTTGAATTAGGTTCACGAAT+TGG	0.407713	1_3:+6301405	Msa0099920:CDS
GCGGTGGTGGTTATAAGTTT+AGG	0.412585	1_3:+6301577	Msa0099920:CDS
GGAATGGTGTTGGTGGAAAT+GGG	0.413937	1_3:+6301712	Msa0099920:CDS
GTGTTGGTGGAAATGGGAAT+GGG	0.429746	1_3:+6301718	Msa0099920:CDS
GGAGTTGATGACGGTGGTGG+TGG	0.440641	1_3:+6301672	Msa0099920:CDS
CCGATTGGATTCGCTTCGCT+GGG	0.440816	1_3:+6301651	Msa0099920:CDS
GGTGAGGGGAATGGTGTTGG+TGG	0.474033	1_3:+6301705	Msa0099920:CDS
CCCAGCGAAGCGAATCCAAT+CGG	0.484023	1_3:-6301651	None:intergenic
TAATTAGAAAGAAGTGAAGC+TGG	0.489003	1_3:-6301483	None:intergenic
GGTGTTGGTGGAAATGGGAA+TGG	0.504989	1_3:+6301717	Msa0099920:CDS
AGTTTAAACAATAGCGGTGG+TGG	0.514164	1_3:+6301564	Msa0099920:CDS
CTGTTAGGGTTCATTGTGAG+AGG	0.529931	1_3:+6301625	Msa0099920:CDS
TGGGGTTGTGTGGAAGATGA+AGG	0.530487	1_3:+6301737	Msa0099920:CDS
GAAATGGGAATGGGGTTGTG+TGG	0.535202	1_3:+6301727	Msa0099920:CDS
ATTGTTTAAACTGAGTGAAA+CGG	0.538925	1_3:-6301553	None:intergenic
GTGTGAGGTTGTTGAAGTAA+AGG	0.540601	1_3:-6301506	None:intergenic
CTGGGAGTTGATGACGGTGG+TGG	0.541803	1_3:+6301669	Msa0099920:CDS
TGTGTGGAAGATGAAGGATT+GGG	0.541858	1_3:+6301743	Msa0099920:CDS
TTGTGTGGAAGATGAAGGAT+TGG	0.550620	1_3:+6301742	Msa0099920:CDS
TGTTGGTGGAAATGGGAATG+GGG	0.551450	1_3:+6301719	Msa0099920:CDS
GCTTCGCTGGGAGTTGATGA+CGG	0.562992	1_3:+6301663	Msa0099920:CDS
CATTGTGAGAGGATTCCGAT+TGG	0.571847	1_3:+6301636	Msa0099920:CDS
TCACTCAGTTTAAACAATAG+CGG	0.624973	1_3:+6301558	Msa0099920:CDS
CTTCAACAACCTCACACGTG+TGG	0.626249	1_3:+6301512	Msa0099920:CDS
CTCAGTTTAAACAATAGCGG+TGG	0.637662	1_3:+6301561	Msa0099920:CDS
ATGATGATGCCACACGTGTG+AGG	0.673938	1_3:-6301521	None:intergenic
TCGCTGGGAGTTGATGACGG+TGG	0.773048	1_3:+6301666	Msa0099920:CDS
TGTTACAAAAGAACCCAATG+CGG	0.774333	1_3:+6301380	Msa0099920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTGTTTAAACTGAGTGAAA+CGG	-	chr1_3:6301556-6301575	None:intergenic	25.0%
!!!	ATGATTTTAATAGAGCTGTT+AGG	+	chr1_3:6301610-6301629	Msa0099920:CDS	25.0%
!!!	TGATTTTAATAGAGCTGTTA+GGG	+	chr1_3:6301611-6301630	Msa0099920:CDS	25.0%
	TTTGAATTAGGTTCACGAAT+TGG	+	chr1_3:6301405-6301424	Msa0099920:CDS	30.0%
	TAATTAGAAAGAAGTGAAGC+TGG	-	chr1_3:6301486-6301505	None:intergenic	30.0%
	TCACTCAGTTTAAACAATAG+CGG	+	chr1_3:6301558-6301577	Msa0099920:CDS	30.0%
	TGTTACAAAAGAACCCAATG+CGG	+	chr1_3:6301380-6301399	Msa0099920:CDS	35.0%
	CCTAATTCAAAAACCGCATT+GGG	-	chr1_3:6301396-6301415	None:intergenic	35.0%
	ACCTAATTCAAAAACCGCAT+TGG	-	chr1_3:6301397-6301416	None:intergenic	35.0%
!	CCCAATGCGGTTTTTGAATT+AGG	+	chr1_3:6301393-6301412	Msa0099920:CDS	40.0%
!!	GTGAACATGCTTCTGAAGTT+AGG	-	chr1_3:6301464-6301483	None:intergenic	40.0%
	GTGTGAGGTTGTTGAAGTAA+AGG	-	chr1_3:6301509-6301528	None:intergenic	40.0%
	CTCAGTTTAAACAATAGCGG+TGG	+	chr1_3:6301561-6301580	Msa0099920:CDS	40.0%
	AGTTTAAACAATAGCGGTGG+TGG	+	chr1_3:6301564-6301583	Msa0099920:CDS	40.0%
	TTGTGTGGAAGATGAAGGAT+TGG	+	chr1_3:6301742-6301761	Msa0099920:CDS	40.0%
	TGTGTGGAAGATGAAGGATT+GGG	+	chr1_3:6301743-6301762	Msa0099920:CDS	40.0%
!	TCTTTTGTAACACGCGTAGC+TGG	-	chr1_3:6301372-6301391	None:intergenic	45.0%
!	GCGGTGGTGGTTATAAGTTT+AGG	+	chr1_3:6301577-6301596	Msa0099920:CDS	45.0%
	CTGTTAGGGTTCATTGTGAG+AGG	+	chr1_3:6301625-6301644	Msa0099920:CDS	45.0%
	CATTGTGAGAGGATTCCGAT+TGG	+	chr1_3:6301636-6301655	Msa0099920:CDS	45.0%
!!	GGAATGGTGTTGGTGGAAAT+GGG	+	chr1_3:6301712-6301731	Msa0099920:CDS	45.0%
!	GTGTTGGTGGAAATGGGAAT+GGG	+	chr1_3:6301718-6301737	Msa0099920:CDS	45.0%
!	TGTTGGTGGAAATGGGAATG+GGG	+	chr1_3:6301719-6301738	Msa0099920:CDS	45.0%
	CTTCAACAACCTCACACGTG+TGG	+	chr1_3:6301512-6301531	Msa0099920:CDS	50.0%
!	ATGATGATGCCACACGTGTG+AGG	-	chr1_3:6301524-6301543	None:intergenic	50.0%
!!	GGGAATGGTGTTGGTGGAAA+TGG	+	chr1_3:6301711-6301730	Msa0099920:CDS	50.0%
!!	GGTGTTGGTGGAAATGGGAA+TGG	+	chr1_3:6301717-6301736	Msa0099920:CDS	50.0%
	GAAATGGGAATGGGGTTGTG+TGG	+	chr1_3:6301727-6301746	Msa0099920:CDS	50.0%
	TGGGGTTGTGTGGAAGATGA+AGG	+	chr1_3:6301737-6301756	Msa0099920:CDS	50.0%
	TCCGATTGGATTCGCTTCGC+TGG	+	chr1_3:6301650-6301669	Msa0099920:CDS	55.0%
	CCCAGCGAAGCGAATCCAAT+CGG	-	chr1_3:6301654-6301673	None:intergenic	55.0%
	CCGATTGGATTCGCTTCGCT+GGG	+	chr1_3:6301651-6301670	Msa0099920:CDS	55.0%
!!	GCTTCGCTGGGAGTTGATGA+CGG	+	chr1_3:6301663-6301682	Msa0099920:CDS	55.0%
!!	TCGCTGGGAGTTGATGACGG+TGG	+	chr1_3:6301666-6301685	Msa0099920:CDS	60.0%
!!	CTGGGAGTTGATGACGGTGG+TGG	+	chr1_3:6301669-6301688	Msa0099920:CDS	60.0%
!!	GGAGTTGATGACGGTGGTGG+TGG	+	chr1_3:6301672-6301691	Msa0099920:CDS	60.0%
!!	GTTGATGACGGTGGTGGTGG+TGG	+	chr1_3:6301675-6301694	Msa0099920:CDS	60.0%
!!	TGGTGGTGGTGTTGGTGGTG+AGG	+	chr1_3:6301689-6301708	Msa0099920:CDS	60.0%
!!	GGTGGTGGTGTTGGTGGTGA+GGG	+	chr1_3:6301690-6301709	Msa0099920:CDS	60.0%
!!	GTGGTGGTGTTGGTGGTGAG+GGG	+	chr1_3:6301691-6301710	Msa0099920:CDS	60.0%
!!	GGTGTTGGTGGTGAGGGGAA+TGG	+	chr1_3:6301696-6301715	Msa0099920:CDS	60.0%
	GGTGGTGAGGGGAATGGTGT+TGG	+	chr1_3:6301702-6301721	Msa0099920:CDS	60.0%
!	GGTGAGGGGAATGGTGTTGG+TGG	+	chr1_3:6301705-6301724	Msa0099920:CDS	60.0%
!!	GACGGTGGTGGTGGTGGTGT+TGG	+	chr1_3:6301681-6301700	Msa0099920:CDS	65.0%
!!	GGTGGTGGTGGTGGTGTTGG+TGG	+	chr1_3:6301684-6301703	Msa0099920:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	6301360	6301770	6301360	ID=Msa0099920;Name=Msa0099920
chr1_3	mRNA	6301360	6301770	6301360	ID=Msa0099920-mRNA-1;Parent=Msa0099920;Name=Msa0099920-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
chr1_3	exon	6301360	6301770	6301360	ID=Msa0099920-mRNA-1:exon:2393;Parent=Msa0099920-mRNA-1
chr1_3	CDS	6301360	6301770	6301360	ID=Msa0099920-mRNA-1:cds;Parent=Msa0099920-mRNA-1

Gene Sequence

>Msa0099920

ATGTATAATCCAGCTACGCGTGTTACAAAAGAACCCAATGCGGTTTTTGAATTAGGTTCACGAATTGGTCGATTCACACTCGATACGTCGTCGTTTTTAAACCTAACTTCAGAAGCATGTTCACCAGCTTCACTTCTTTCTAATTACCTTTACTTCAACAACCTCACACGTGTGGCATCATCATCAAAACGCACCGTTTCACTCAGTTTAAACAATAGCGGTGGTGGTTATAAGTTTAGGAATATTCTTCATGATTTTAATAGAGCTGTTAGGGTTCATTGTGAGAGGATTCCGATTGGATTCGCTTCGCTGGGAGTTGATGACGGTGGTGGTGGTGGTGTTGGTGGTGAGGGGAATGGTGTTGGTGGAAATGGGAATGGGGTTGTGTGGAAGATGAAGGATTGGGATTGA

Protein sequence

>Msa0099920

MYNPATRVTKEPNAVFELGSRIGRFTLDTSSFLNLTSEACSPASLLSNYLYFNNLTRVASSSKRTVSLSLNNSGGGYKFRNILHDFNRAVRVHCERIPIGFASLGVDDGGGGGVGGEGNGVGGNGNGVVWKMKDWD*