AlfalfaGEDB | Msa1219520

M. sativa cultivar zhongmu-4 / Msa1219520

KEGG: K07198
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219520	sp\|Q8RWC9\|CIPK1_ARATH	70.047	424	125	2	19	441	13	435	0.0	589

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219520	G7JR06	96.861	446	14	0	1	446	1	446	0.0	882

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027390	Msa1219520	0.820276	7.471266e-53	-8.615850e-47
Msa0050730	Msa1219520	0.827356	1.637326e-54	-8.615850e-47
Msa0050760	Msa1219520	0.821208	4.561256e-53	-8.615850e-47
Msa0085720	Msa1219520	0.803784	2.955403e-49	-8.615850e-47
Msa0099920	Msa1219520	0.803613	3.206935e-49	-8.615850e-47
Msa0102750	Msa1219520	0.809102	2.233249e-50	-8.615850e-47
Msa0105420	Msa1219520	0.809388	1.939797e-50	-8.615850e-47
Msa0122810	Msa1219520	0.800533	1.378372e-48	-8.615850e-47
Msa0165300	Msa1219520	0.826466	2.671789e-54	-8.615850e-47
Msa0200560	Msa1219520	0.802650	5.072996e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219520	MtrunA17_Chr4g0069301	96.861	446	14	0	1	446	1	446	0.0	882
Msa1219520	MtrunA17_Chr2g0302431	75.056	445	102	3	1	443	1	438	0.0	663
Msa1219520	MtrunA17_Chr5g0441011	51.529	425	190	3	22	439	9	424	3.56e-157	452
Msa1219520	MtrunA17_Chr2g0304111	50.000	430	204	5	17	439	14	439	6.90e-151	436
Msa1219520	MtrunA17_Chr8g0346081	49.524	420	206	4	21	439	12	426	5.02e-146	423
Msa1219520	MtrunA17_Chr5g0428031	48.700	423	207	6	22	439	5	422	9.73e-146	423
Msa1219520	MtrunA17_Chr4g0063881	46.620	429	217	6	22	442	7	431	1.67e-144	420
Msa1219520	MtrunA17_Chr8g0346071	48.694	421	209	4	14	433	5	419	5.21e-143	416
Msa1219520	MtrunA17_Chr4g0013131	47.242	417	213	4	21	434	7	419	9.09e-132	388
Msa1219520	MtrunA17_Chr7g0244521	46.081	421	217	5	21	434	7	424	2.61e-131	387

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219520	AT3G17510.1	70.047	424	125	2	19	441	13	435	0.0	589
Msa1219520	AT1G48260.1	64.929	422	145	1	19	440	4	422	0.0	560
Msa1219520	AT3G17510.2	69.492	354	106	2	89	441	3	355	8.61e-171	484
Msa1219520	AT1G30270.1	52.830	424	189	4	23	439	28	447	3.37e-160	461
Msa1219520	AT1G30270.2	52.830	424	189	4	23	439	28	447	3.96e-160	461
Msa1219520	AT5G21326.1	51.402	428	189	4	22	439	9	427	3.31e-158	454
Msa1219520	AT2G26980.3	52.506	419	195	3	22	439	10	425	3.40e-158	455
Msa1219520	AT2G26980.4	52.506	419	195	3	22	439	20	435	5.50e-158	454
Msa1219520	AT1G48260.2	64.412	340	118	1	101	440	1	337	1.79e-157	449
Msa1219520	AT1G48260.4	64.412	340	118	1	101	440	1	337	1.79e-157	449

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Find 112 sgRNAs with CRISPR-Local

Find 352 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGAATAACAATGGC+AGG	0.504082	8_1:+82437392	Msa1219520:CDS
AAAAGAGAAAAGCAGAACCA+CGG	0.645439	8_1:+82432314	Msa1219520:five_prime_UTR
AAAATGGTGATATTGAACCT+TGG	0.528261	8_1:+82432441	Msa1219520:exon
AAACCAAAATGGTTATCAGC+TGG	0.549443	8_1:+82437329	Msa1219520:CDS
AAACCAGAATAACAATGGCA+GGG	0.627459	8_1:+82437393	Msa1219520:CDS
AAAGAACCTTGAGATCCCTG+TGG	0.720818	8_1:-82436483	None:intergenic
AAAGAGAAAAGCAGAACCAC+GGG	0.564521	8_1:+82432315	Msa1219520:five_prime_UTR
AAATGGTGATATTGAACCTT+GGG	0.445350	8_1:+82432442	Msa1219520:exon
AACAAGGATCATAAAACTCT+TGG	0.499777	8_1:+82438227	Msa1219520:CDS
AAGAAGTTATTAAATGATCT+TGG	0.418743	8_1:+82438537	Msa1219520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGGTAGATAGATAAGACAA+CGG	-	chr8_1:82432268-82432287	None:intergenic	30.0%
AAAATGGTGATATTGAACCT+TGG	+	chr8_1:82432441-82432460	Msa1219520:exon	30.0%
AAATGGTGATATTGAACCTT+GGG	+	chr8_1:82432442-82432461	Msa1219520:exon	30.0%
AATAAGAAGAAGTTGCATAG+TGG	+	chr8_1:82432474-82432493	Msa1219520:CDS	30.0%
AGACTTGGAAAATATGAGTT+GGG	+	chr8_1:82432507-82432526	Msa1219520:CDS	30.0%
TTTGCTTATAATAATGTCGG+AGG	+	chr8_1:82432941-82432960	Msa1219520:intron	30.0%
TTGCTTATAATAATGTCGGA+GGG	+	chr8_1:82432942-82432961	Msa1219520:intron	30.0%
GAGATCCAAAAATAATCAGA+TGG	-	chr8_1:82433436-82433455	None:intergenic	30.0%
AATAAATCCATAGCGCATTT+GGG	-	chr8_1:82433473-82433492	None:intergenic	30.0%
AAATAAATCCATAGCGCATT+TGG	-	chr8_1:82433474-82433493	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	82432125	82438860	82432125	ID=Msa1219520;Name=Msa1219520
chr8_1	mRNA	82432125	82438860	82432125	ID=Msa1219520-mRNA-1;Parent=Msa1219520;Name=Msa1219520-mRNA-1;_AED=0.19;_eAED=0.19;_QI=319\|1\|1\|1\|0.91\|0.84\|13\|225\|446
chr8_1	exon	82432125	82432653	82432125	ID=Msa1219520-mRNA-1:exon:29315;Parent=Msa1219520-mRNA-1
chr8_1	exon	82433298	82433360	82433298	ID=Msa1219520-mRNA-1:exon:29316;Parent=Msa1219520-mRNA-1
chr8_1	exon	82436228	82436299	82436228	ID=Msa1219520-mRNA-1:exon:29317;Parent=Msa1219520-mRNA-1
chr8_1	exon	82436391	82436498	82436391	ID=Msa1219520-mRNA-1:exon:29318;Parent=Msa1219520-mRNA-1
chr8_1	exon	82436856	82436936	82436856	ID=Msa1219520-mRNA-1:exon:29319;Parent=Msa1219520-mRNA-1
chr8_1	exon	82437028	82437207	82437028	ID=Msa1219520-mRNA-1:exon:29320;Parent=Msa1219520-mRNA-1
chr8_1	exon	82437308	82437520	82437308	ID=Msa1219520-mRNA-1:exon:29321;Parent=Msa1219520-mRNA-1
chr8_1	exon	82437736	82437843	82437736	ID=Msa1219520-mRNA-1:exon:29322;Parent=Msa1219520-mRNA-1
chr8_1	exon	82437979	82438095	82437979	ID=Msa1219520-mRNA-1:exon:29323;Parent=Msa1219520-mRNA-1
chr8_1	exon	82438209	82438271	82438209	ID=Msa1219520-mRNA-1:exon:29324;Parent=Msa1219520-mRNA-1
chr8_1	exon	82438367	82438441	82438367	ID=Msa1219520-mRNA-1:exon:29325;Parent=Msa1219520-mRNA-1
chr8_1	exon	82438531	82438663	82438531	ID=Msa1219520-mRNA-1:exon:29326;Parent=Msa1219520-mRNA-1
chr8_1	exon	82438718	82438860	82438718	ID=Msa1219520-mRNA-1:exon:29327;Parent=Msa1219520-mRNA-1
chr8_1	five_prime_UTR	82432125	82432443	82432125	ID=Msa1219520-mRNA-1:five_prime_utr;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82432444	82432653	82432444	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82433298	82433360	82433298	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82436228	82436299	82436228	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82436391	82436498	82436391	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82436856	82436936	82436856	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82437028	82437207	82437028	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82437308	82437520	82437308	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82437736	82437843	82437736	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82437979	82438095	82437979	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82438209	82438271	82438209	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82438367	82438441	82438367	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	CDS	82438531	82438581	82438531	ID=Msa1219520-mRNA-1:cds;Parent=Msa1219520-mRNA-1
chr8_1	three_prime_UTR	82438582	82438663	82438582	ID=Msa1219520-mRNA-1:three_prime_utr;Parent=Msa1219520-mRNA-1
chr8_1	three_prime_UTR	82438718	82438860	82438718	ID=Msa1219520-mRNA-1:three_prime_utr;Parent=Msa1219520-mRNA-1

Gene Sequence

>Msa1219520

ATGGTGATATTGAACCTTGGGAAGAAGCAGAATAAGAAGAAGTTGCATAGTGGAGAAGGAGTGAGACTTGGAAAATATGAGTTGGGTAGGACTCTTGGTGAGGGCAATTTTGGTAAAGTCAAGTTTGCTAGGAACACTGATTCTGGACAACCTTATGCTATTAAGATTATTGACAAGAACAGGATCATTGATCTCAATATCACAAACCAGATAAAGAGGGAAATATGTGCCTTGAAGCTCCTAAGGCATCCCAATGTTGTTAAATTATACGAGGTCTTAGCTAGCAAAACAAAAATTTACATGGTACTTGAGTACGTGACAGGAGAGGAGTTATTTGACAAAATAGCTTCCAAGGGTAAACTTACTGAAGGTGAAGGTAGAAAATTGTTCCAACAGTTGATTGATGGTGTGAGCTACTGCCACAGTAAAGGTGTCTTCCACAGGGATCTCAAGCTTGAGAATGTACTTGTGGATGCCAAAGGGAATCTAAAGATAACTGATTTTGGTCTTAGTGCTTTACCCCAACAATTTAGGGCAGATGGGTTGTTGCATACAACATGTGGAAGTGCAAATTATGTTGCACCAGAGATTCTTGCTAATAGGGGCTATAATGGTGCATCATCAGATGTATGGTCATGTGGTGTTATCTTGTATGTAGTCTTAACAGGATTCCTACCTTTTGATGATAGAAATCTTGCAGTTCTCTATCAGAAGGTTTTGAAAGGAGATTTTCAGAAACCAAAATGGTTATCAGCTGGTGCACAAAACATTATAAAGAGGATTCTTGATCCCAACCCTAAAACCAGAATAACAATGGCAGGGATCAAAGAAGATTTGTGGTTCAAGGAAGGATATAATCAAGCAGATCACGAGGACGAGGAAGACAATGTATATGTTGACGATCAAGCCTTTAACATCCATGAATTGTCACAGGAAGAAGAAAAGAGTAGTTCAGGATCACCAATTCGTATCAATGCATTTCAGTTGATAGGGATGTCTTCATGCCTAGACCTCTCTGGCTTTTTTGAGAAAGAGGATGTATCTGAGAGGAAGATAAGGTTTACAACTAATCTTTCAGCCAAAGATCTGATGGAGAAGATTGAAGATTCTGTGACAGAAATGGAATTTAGAGTTCAAAAGAAAAATGGCAAAATGAAAGTGATACAAGAGAACAAGGATCATAAAACTCTTGGCAGCCTCTCAGTGATAATAGAGGTTTTTGAAATAAGTTCATCATTGTATTTAGTAGAATTAAGGAAGTGTTATGGAGATGCCTCTGTATATAGACAGCTATGCAAGAAGTTATTAAATGATCTTGGTGTTCCCCAAGGAAAGCACTAG

Protein sequence

>Msa1219520

MVILNLGKKQNKKKLHSGEGVRLGKYELGRTLGEGNFGKVKFARNTDSGQPYAIKIIDKNRIIDLNITNQIKREICALKLLRHPNVVKLYEVLASKTKIYMVLEYVTGEELFDKIASKGKLTEGEGRKLFQQLIDGVSYCHSKGVFHRDLKLENVLVDAKGNLKITDFGLSALPQQFRADGLLHTTCGSANYVAPEILANRGYNGASSDVWSCGVILYVVLTGFLPFDDRNLAVLYQKVLKGDFQKPKWLSAGAQNIIKRILDPNPKTRITMAGIKEDLWFKEGYNQADHEDEEDNVYVDDQAFNIHELSQEEEKSSSGSPIRINAFQLIGMSSCLDLSGFFEKEDVSERKIRFTTNLSAKDLMEKIEDSVTEMEFRVQKKNGKMKVIQENKDHKTLGSLSVIIEVFEISSSLYLVELRKCYGDASVYRQLCKKLLNDLGVPQGKH*