AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0108700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108700 MtrunA17_Chr1g0146901 85.149 101 7 2 1 101 1 93 4.18e-55 166
Msa0108700 MtrunA17_Chr3g0127221 84.158 101 10 2 1 101 1 95 1.26e-54 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108700 AT5G53045.1 67.368 95 25 2 3 97 7 95 4.36e-39 125

Find 39 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGACCCACTTCCTTAT+TGG 0.185735 1_3:-20295072 None:intergenic
ACCAAAACAATTGAAGAATT+TGG 0.196365 1_3:+20296429 Msa0108700:CDS
ACCAAATTCTTCAATTGTTT+TGG 0.227496 1_3:-20296430 None:intergenic
TTTATGTGTTATGAATTAAA+AGG 0.227662 1_3:-20296595 None:intergenic
ATGCACACTCAAATTTATAT+TGG 0.236305 1_3:-20295109 None:intergenic
AGTGACTTGGAAGTGGATTT+TGG 0.302231 1_3:+20295586 Msa0108700:CDS
TTGAATTAGTCATCTTGAAT+TGG 0.327106 1_3:+20295017 Msa0108700:five_prime_UTR
CGACTCATGACGGTGTCTAA+TGG 0.332701 1_3:+20295772 Msa0108700:CDS
TGCACACTCAAATTTATATT+GGG 0.338729 1_3:-20295108 None:intergenic
AATGGAGCTGTGACTTCTTA+TGG 0.358258 1_3:+20296458 Msa0108700:exon
ATGAGTCGTTTAACTTTGTC+AGG 0.393714 1_3:-20295757 None:intergenic
GAATGATGCTCGAAGAAATC+TGG 0.394790 1_3:-20296376 None:intergenic
CTTTATATTTAGGCACTTTG+AGG 0.444146 1_3:+20296344 Msa0108700:intron
TTATGTGTTATGAATTAAAA+GGG 0.461490 1_3:-20296594 None:intergenic
CTCAAATTTATATTGGGAGC+GGG 0.485515 1_3:-20295102 None:intergenic
TTTATTAAGAAACACATCTC+AGG 0.489313 1_3:-20296563 None:intergenic
TGGACTATCCAATAAGGAAG+TGG 0.498479 1_3:+20295064 Msa0108700:five_prime_UTR
TGCTGCCTTAGCTGTGGATG+AGG 0.502660 1_3:+20295636 Msa0108700:CDS
TGAAGAATTTGGTCAAGGAA+TGG 0.505507 1_3:+20296440 Msa0108700:CDS
AAACCTGGTAAAATCCCATT+GGG 0.510542 1_3:-20295228 None:intergenic
GAAATAGTACATTGAAACAT+TGG 0.514102 1_3:-20296488 None:intergenic
TGGTAAAATCCCATTGGGAT+TGG 0.519873 1_3:-20295223 None:intergenic
ACCACACAACCAATCCCAAT+GGG 0.522672 1_3:+20295214 Msa0108700:CDS
GAATGACAATTGCAGTGACT+TGG 0.538867 1_3:+20295573 Msa0108700:CDS
GAAACCTGGTAAAATCCCAT+TGG 0.539649 1_3:-20295229 None:intergenic
CAATTGCAGTGACTTGGAAG+TGG 0.541986 1_3:+20295579 Msa0108700:CDS
CACCTCCTCATCCACAGCTA+AGG 0.544761 1_3:-20295641 None:intergenic
TTGGGATTGGTTGTGTGGTG+TGG 0.553170 1_3:-20295210 None:intergenic
TCCCATTGGGATTGGTTGTG+TGG 0.555381 1_3:-20295215 None:intergenic
GGACTATCCAATAAGGAAGT+GGG 0.559011 1_3:+20295065 Msa0108700:five_prime_UTR
ACAATTGAAGAATTTGGTCA+AGG 0.561595 1_3:+20296435 Msa0108700:CDS
CACCACACAACCAATCCCAA+TGG 0.580515 1_3:+20295213 Msa0108700:CDS
TGTATATGCTGCCTTAGCTG+TGG 0.587580 1_3:+20295630 Msa0108700:CDS
ACTCAAATTTATATTGGGAG+CGG 0.594457 1_3:-20295103 None:intergenic
CTTCGCGCTTGAAGTTGTTG+CGG 0.604354 1_3:+20295161 Msa0108700:five_prime_UTR
CAGAGTAGAGTAGTCACACA+TGG 0.604563 1_3:+20295184 Msa0108700:exon
CAAAGTTAAACGACTCATGA+CGG 0.619519 1_3:+20295762 Msa0108700:CDS
TGCCTTAGCTGTGGATGAGG+AGG 0.626509 1_3:+20295639 Msa0108700:CDS
AGTTGTGAGACATAGAAACG+AGG 0.773551 1_3:+20296635 Msa0108700:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTATTTTAAAGAATTG+TGG + chr1_3:20295483-20295502 Msa0108700:intron 10.0%
!!! TAATTATTTTAAAGAATTGT+GGG + chr1_3:20295484-20295503 Msa0108700:intron 10.0%
!!! ATTGTTATTTTTAATAAAGA+TGG + chr1_3:20295551-20295570 Msa0108700:intron 10.0%
!!! TTAATTATTTTAAAGAATTG+TGG + chr1_3:20295483-20295502 Msa0108700:intron 10.0%
!!! TAATTATTTTAAAGAATTGT+GGG + chr1_3:20295484-20295503 Msa0108700:intron 10.0%
!!! ATTGTTATTTTTAATAAAGA+TGG + chr1_3:20295551-20295570 Msa0108700:intron 10.0%
!! AGATTAAATTAATTAATCAC+TGG - chr1_3:20295324-20295343 None:intergenic 15.0%
!!! ATTAGTTAATTTAGCTTTAA+TGG + chr1_3:20296260-20296279 Msa0108700:intron 15.0%
!! TTATGTGTTATGAATTAAAA+GGG - chr1_3:20296597-20296616 None:intergenic 15.0%
!! TTTATGTGTTATGAATTAAA+AGG - chr1_3:20296598-20296617 None:intergenic 15.0%
!! AGATTAAATTAATTAATCAC+TGG - chr1_3:20295324-20295343 None:intergenic 15.0%
!!! ATTAGTTAATTTAGCTTTAA+TGG + chr1_3:20296260-20296279 Msa0108700:intron 15.0%
!! TTATGTGTTATGAATTAAAA+GGG - chr1_3:20296597-20296616 None:intergenic 15.0%
!! TTTATGTGTTATGAATTAAA+AGG - chr1_3:20296598-20296617 None:intergenic 15.0%
!!! TGATTTTTTGCTTAACTTTT+TGG + chr1_3:20295044-20295063 Msa0108700:five_prime_UTR 20.0%
!! GCTTATTTACATAATTTCTA+GGG - chr1_3:20295385-20295404 None:intergenic 20.0%
!! AGCTTATTTACATAATTTCT+AGG - chr1_3:20295386-20295405 None:intergenic 20.0%
!!! ATGTACTATTTCTTTTTGTA+TGG + chr1_3:20296499-20296518 Msa0108700:three_prime_UTR 20.0%
!!! TGATTTTTTGCTTAACTTTT+TGG + chr1_3:20295044-20295063 Msa0108700:five_prime_UTR 20.0%
!! GCTTATTTACATAATTTCTA+GGG - chr1_3:20295385-20295404 None:intergenic 20.0%
!! AGCTTATTTACATAATTTCT+AGG - chr1_3:20295386-20295405 None:intergenic 20.0%
!!! ATGTACTATTTCTTTTTGTA+TGG + chr1_3:20296499-20296518 Msa0108700:three_prime_UTR 20.0%
! TTGAATTAGTCATCTTGAAT+TGG + chr1_3:20295017-20295036 Msa0108700:five_prime_UTR 25.0%
! TGCACACTCAAATTTATATT+GGG - chr1_3:20295111-20295130 None:intergenic 25.0%
! ATGCACACTCAAATTTATAT+TGG - chr1_3:20295112-20295131 None:intergenic 25.0%
! AAAAAGCAAGAAAACTTGAT+AGG - chr1_3:20295282-20295301 None:intergenic 25.0%
!! GATTTCTTTTGTCTTATGAA+CGG - chr1_3:20295457-20295476 None:intergenic 25.0%
!!! ACTGCAATGATTTTTTTTGT+TGG + chr1_3:20296187-20296206 Msa0108700:intron 25.0%
! CATTGGAATCCTTTATATTT+AGG + chr1_3:20296334-20296353 Msa0108700:intron 25.0%
! ACCAAAACAATTGAAGAATT+TGG + chr1_3:20296429-20296448 Msa0108700:CDS 25.0%
!! ACCAAATTCTTCAATTGTTT+TGG - chr1_3:20296433-20296452 None:intergenic 25.0%
! GAAATAGTACATTGAAACAT+TGG - chr1_3:20296491-20296510 None:intergenic 25.0%
! TTTATTAAGAAACACATCTC+AGG - chr1_3:20296566-20296585 None:intergenic 25.0%
!!! CGAGGTTTATTTTCTTTTTT+TGG + chr1_3:20296653-20296672 Msa0108700:three_prime_UTR 25.0%
! TTGAATTAGTCATCTTGAAT+TGG + chr1_3:20295017-20295036 Msa0108700:five_prime_UTR 25.0%
! TGCACACTCAAATTTATATT+GGG - chr1_3:20295111-20295130 None:intergenic 25.0%
! ATGCACACTCAAATTTATAT+TGG - chr1_3:20295112-20295131 None:intergenic 25.0%
! AAAAAGCAAGAAAACTTGAT+AGG - chr1_3:20295282-20295301 None:intergenic 25.0%
!! GATTTCTTTTGTCTTATGAA+CGG - chr1_3:20295457-20295476 None:intergenic 25.0%
!!! ACTGCAATGATTTTTTTTGT+TGG + chr1_3:20296187-20296206 Msa0108700:intron 25.0%
! CATTGGAATCCTTTATATTT+AGG + chr1_3:20296334-20296353 Msa0108700:intron 25.0%
! ACCAAAACAATTGAAGAATT+TGG + chr1_3:20296429-20296448 Msa0108700:CDS 25.0%
!! ACCAAATTCTTCAATTGTTT+TGG - chr1_3:20296433-20296452 None:intergenic 25.0%
! GAAATAGTACATTGAAACAT+TGG - chr1_3:20296491-20296510 None:intergenic 25.0%
! TTTATTAAGAAACACATCTC+AGG - chr1_3:20296566-20296585 None:intergenic 25.0%
!!! CGAGGTTTATTTTCTTTTTT+TGG + chr1_3:20296653-20296672 Msa0108700:three_prime_UTR 25.0%
! ACTTTTTGGACTATCCAATA+AGG + chr1_3:20295058-20295077 Msa0108700:five_prime_UTR 30.0%
ACTCAAATTTATATTGGGAG+CGG - chr1_3:20295106-20295125 None:intergenic 30.0%
!!! TTCCTTTTCGTGTTGATAAT+TGG + chr1_3:20295814-20295833 Msa0108700:intron 30.0%
AACCAATTATCAACACGAAA+AGG - chr1_3:20295819-20295838 None:intergenic 30.0%
!!! GTTCAATGATTGTTTTAGTC+AGG + chr1_3:20296067-20296086 Msa0108700:intron 30.0%
CAGTTAGTGACAAATTGTTT+AGG - chr1_3:20296110-20296129 None:intergenic 30.0%
TTCCAATGAGATGAAAAACT+AGG - chr1_3:20296322-20296341 None:intergenic 30.0%
! CTCAAAGTGCCTAAATATAA+AGG - chr1_3:20296346-20296365 None:intergenic 30.0%
CTTTATATTTAGGCACTTTG+AGG + chr1_3:20296344-20296363 Msa0108700:intron 30.0%
ACAATTGAAGAATTTGGTCA+AGG + chr1_3:20296435-20296454 Msa0108700:CDS 30.0%
!!! CAAGACATTTTTTGTATGCA+TGG + chr1_3:20296531-20296550 Msa0108700:three_prime_UTR 30.0%
! ACTTTTTGGACTATCCAATA+AGG + chr1_3:20295058-20295077 Msa0108700:five_prime_UTR 30.0%
ACTCAAATTTATATTGGGAG+CGG - chr1_3:20295106-20295125 None:intergenic 30.0%
!!! TTCCTTTTCGTGTTGATAAT+TGG + chr1_3:20295814-20295833 Msa0108700:intron 30.0%
AACCAATTATCAACACGAAA+AGG - chr1_3:20295819-20295838 None:intergenic 30.0%
!!! GTTCAATGATTGTTTTAGTC+AGG + chr1_3:20296067-20296086 Msa0108700:intron 30.0%
CAGTTAGTGACAAATTGTTT+AGG - chr1_3:20296110-20296129 None:intergenic 30.0%
TTCCAATGAGATGAAAAACT+AGG - chr1_3:20296322-20296341 None:intergenic 30.0%
! CTCAAAGTGCCTAAATATAA+AGG - chr1_3:20296346-20296365 None:intergenic 30.0%
CTTTATATTTAGGCACTTTG+AGG + chr1_3:20296344-20296363 Msa0108700:intron 30.0%
ACAATTGAAGAATTTGGTCA+AGG + chr1_3:20296435-20296454 Msa0108700:CDS 30.0%
!!! CAAGACATTTTTTGTATGCA+TGG + chr1_3:20296531-20296550 Msa0108700:three_prime_UTR 30.0%
CTCAAATTTATATTGGGAGC+GGG - chr1_3:20295105-20295124 None:intergenic 35.0%
AAACCTGGTAAAATCCCATT+GGG - chr1_3:20295231-20295250 None:intergenic 35.0%
! ATGAGTCGTTTAACTTTGTC+AGG - chr1_3:20295760-20295779 None:intergenic 35.0%
CAAAGTTAAACGACTCATGA+CGG + chr1_3:20295762-20295781 Msa0108700:CDS 35.0%
! TGTAATCAATTTTGTGTGCG+AGG + chr1_3:20295925-20295944 Msa0108700:intron 35.0%
TCTTTCTCACGAGAATAAGA+GGG - chr1_3:20296013-20296032 None:intergenic 35.0%
TTCTTTCTCACGAGAATAAG+AGG - chr1_3:20296014-20296033 None:intergenic 35.0%
TTTAGTCAGGATTTGAACTC+AGG + chr1_3:20296080-20296099 Msa0108700:intron 35.0%
TCATTGTACTGCATCATGTT+TGG + chr1_3:20296160-20296179 Msa0108700:intron 35.0%
!! TGCCTAGTTTTTCATCTCAT+TGG + chr1_3:20296317-20296336 Msa0108700:intron 35.0%
! TTTTGTAGATGTCCTTACTC+TGG + chr1_3:20296404-20296423 Msa0108700:CDS 35.0%
TGAAGAATTTGGTCAAGGAA+TGG + chr1_3:20296440-20296459 Msa0108700:CDS 35.0%
!!! GACATTTTTTGTATGCATGG+AGG + chr1_3:20296534-20296553 Msa0108700:three_prime_UTR 35.0%
CTCAAATTTATATTGGGAGC+GGG - chr1_3:20295105-20295124 None:intergenic 35.0%
AAACCTGGTAAAATCCCATT+GGG - chr1_3:20295231-20295250 None:intergenic 35.0%
! ATGAGTCGTTTAACTTTGTC+AGG - chr1_3:20295760-20295779 None:intergenic 35.0%
CAAAGTTAAACGACTCATGA+CGG + chr1_3:20295762-20295781 Msa0108700:CDS 35.0%
! TGTAATCAATTTTGTGTGCG+AGG + chr1_3:20295925-20295944 Msa0108700:intron 35.0%
TCTTTCTCACGAGAATAAGA+GGG - chr1_3:20296013-20296032 None:intergenic 35.0%
TTCTTTCTCACGAGAATAAG+AGG - chr1_3:20296014-20296033 None:intergenic 35.0%
TTTAGTCAGGATTTGAACTC+AGG + chr1_3:20296080-20296099 Msa0108700:intron 35.0%
TCATTGTACTGCATCATGTT+TGG + chr1_3:20296160-20296179 Msa0108700:intron 35.0%
!! TGCCTAGTTTTTCATCTCAT+TGG + chr1_3:20296317-20296336 Msa0108700:intron 35.0%
! TTTTGTAGATGTCCTTACTC+TGG + chr1_3:20296404-20296423 Msa0108700:CDS 35.0%
TGAAGAATTTGGTCAAGGAA+TGG + chr1_3:20296440-20296459 Msa0108700:CDS 35.0%
!!! GACATTTTTTGTATGCATGG+AGG + chr1_3:20296534-20296553 Msa0108700:three_prime_UTR 35.0%
TGGACTATCCAATAAGGAAG+TGG + chr1_3:20295064-20295083 Msa0108700:five_prime_UTR 40.0%
GGACTATCCAATAAGGAAGT+GGG + chr1_3:20295065-20295084 Msa0108700:five_prime_UTR 40.0%
GATTTGACCCACTTCCTTAT+TGG - chr1_3:20295075-20295094 None:intergenic 40.0%
TGGTAAAATCCCATTGGGAT+TGG - chr1_3:20295226-20295245 None:intergenic 40.0%
! AATCCCAATGGGATTTTACC+AGG + chr1_3:20295225-20295244 Msa0108700:CDS 40.0%
GAAACCTGGTAAAATCCCAT+TGG - chr1_3:20295232-20295251 None:intergenic 40.0%
AGAGTAAAGAGAGAGAAACC+TGG - chr1_3:20295246-20295265 None:intergenic 40.0%
GAATGACAATTGCAGTGACT+TGG + chr1_3:20295573-20295592 Msa0108700:CDS 40.0%
!! AGTGACTTGGAAGTGGATTT+TGG + chr1_3:20295586-20295605 Msa0108700:CDS 40.0%
AGAAAGAAGCCCTAGTAATC+CGG + chr1_3:20296026-20296045 Msa0108700:intron 40.0%
TTAACACCACCGGATTACTA+GGG - chr1_3:20296038-20296057 None:intergenic 40.0%
CAGACTTTACTTAACACCAC+CGG - chr1_3:20296048-20296067 None:intergenic 40.0%
ATGAAGTGGTTGGACTCAAA+TGG - chr1_3:20296144-20296163 None:intergenic 40.0%
ATGCAGTACAATGAAGTGGT+TGG - chr1_3:20296154-20296173 None:intergenic 40.0%
CATGATGCAGTACAATGAAG+TGG - chr1_3:20296158-20296177 None:intergenic 40.0%
GAATGATGCTCGAAGAAATC+TGG - chr1_3:20296379-20296398 None:intergenic 40.0%
!!! TTGTTTTGGTTGCCAGAGTA+AGG - chr1_3:20296419-20296438 None:intergenic 40.0%
AATGGAGCTGTGACTTCTTA+TGG + chr1_3:20296458-20296477 Msa0108700:exon 40.0%
AGTTGTGAGACATAGAAACG+AGG + chr1_3:20296635-20296654 Msa0108700:three_prime_UTR 40.0%
TGGACTATCCAATAAGGAAG+TGG + chr1_3:20295064-20295083 Msa0108700:five_prime_UTR 40.0%
GGACTATCCAATAAGGAAGT+GGG + chr1_3:20295065-20295084 Msa0108700:five_prime_UTR 40.0%
GATTTGACCCACTTCCTTAT+TGG - chr1_3:20295075-20295094 None:intergenic 40.0%
TGGTAAAATCCCATTGGGAT+TGG - chr1_3:20295226-20295245 None:intergenic 40.0%
! AATCCCAATGGGATTTTACC+AGG + chr1_3:20295225-20295244 Msa0108700:CDS 40.0%
GAAACCTGGTAAAATCCCAT+TGG - chr1_3:20295232-20295251 None:intergenic 40.0%
AGAGTAAAGAGAGAGAAACC+TGG - chr1_3:20295246-20295265 None:intergenic 40.0%
GAATGACAATTGCAGTGACT+TGG + chr1_3:20295573-20295592 Msa0108700:CDS 40.0%
!! AGTGACTTGGAAGTGGATTT+TGG + chr1_3:20295586-20295605 Msa0108700:CDS 40.0%
AGAAAGAAGCCCTAGTAATC+CGG + chr1_3:20296026-20296045 Msa0108700:intron 40.0%
TTAACACCACCGGATTACTA+GGG - chr1_3:20296038-20296057 None:intergenic 40.0%
CAGACTTTACTTAACACCAC+CGG - chr1_3:20296048-20296067 None:intergenic 40.0%
ATGAAGTGGTTGGACTCAAA+TGG - chr1_3:20296144-20296163 None:intergenic 40.0%
ATGCAGTACAATGAAGTGGT+TGG - chr1_3:20296154-20296173 None:intergenic 40.0%
CATGATGCAGTACAATGAAG+TGG - chr1_3:20296158-20296177 None:intergenic 40.0%
GAATGATGCTCGAAGAAATC+TGG - chr1_3:20296379-20296398 None:intergenic 40.0%
!!! TTGTTTTGGTTGCCAGAGTA+AGG - chr1_3:20296419-20296438 None:intergenic 40.0%
AATGGAGCTGTGACTTCTTA+TGG + chr1_3:20296458-20296477 Msa0108700:exon 40.0%
AGTTGTGAGACATAGAAACG+AGG + chr1_3:20296635-20296654 Msa0108700:three_prime_UTR 40.0%
CAGAGTAGAGTAGTCACACA+TGG + chr1_3:20295184-20295203 Msa0108700:exon 45.0%
ACCACACAACCAATCCCAAT+GGG + chr1_3:20295214-20295233 Msa0108700:CDS 45.0%
! CAATTGCAGTGACTTGGAAG+TGG + chr1_3:20295579-20295598 Msa0108700:CDS 45.0%
TGTATATGCTGCCTTAGCTG+TGG + chr1_3:20295630-20295649 Msa0108700:CDS 45.0%
CTTAACACCACCGGATTACT+AGG - chr1_3:20296039-20296058 None:intergenic 45.0%
CAGAGTAGAGTAGTCACACA+TGG + chr1_3:20295184-20295203 Msa0108700:exon 45.0%
ACCACACAACCAATCCCAAT+GGG + chr1_3:20295214-20295233 Msa0108700:CDS 45.0%
! CAATTGCAGTGACTTGGAAG+TGG + chr1_3:20295579-20295598 Msa0108700:CDS 45.0%
TGTATATGCTGCCTTAGCTG+TGG + chr1_3:20295630-20295649 Msa0108700:CDS 45.0%
CTTAACACCACCGGATTACT+AGG - chr1_3:20296039-20296058 None:intergenic 45.0%
!! TAAATTGAATTATTAATTAA+TGG - chr1_3:20295422-20295441 None:intergenic 5.0%
!! TAAATTGAATTATTAATTAA+TGG - chr1_3:20295422-20295441 None:intergenic 5.0%
CTTCGCGCTTGAAGTTGTTG+CGG + chr1_3:20295161-20295180 Msa0108700:five_prime_UTR 50.0%
!! TTGGGATTGGTTGTGTGGTG+TGG - chr1_3:20295213-20295232 None:intergenic 50.0%
CACCACACAACCAATCCCAA+TGG + chr1_3:20295213-20295232 Msa0108700:CDS 50.0%
!! TCCCATTGGGATTGGTTGTG+TGG - chr1_3:20295218-20295237 None:intergenic 50.0%
CGACTCATGACGGTGTCTAA+TGG + chr1_3:20295772-20295791 Msa0108700:CDS 50.0%
AAGAAGCCCTAGTAATCCGG+TGG + chr1_3:20296029-20296048 Msa0108700:intron 50.0%
CTTCGCGCTTGAAGTTGTTG+CGG + chr1_3:20295161-20295180 Msa0108700:five_prime_UTR 50.0%
!! TTGGGATTGGTTGTGTGGTG+TGG - chr1_3:20295213-20295232 None:intergenic 50.0%
CACCACACAACCAATCCCAA+TGG + chr1_3:20295213-20295232 Msa0108700:CDS 50.0%
!! TCCCATTGGGATTGGTTGTG+TGG - chr1_3:20295218-20295237 None:intergenic 50.0%
CGACTCATGACGGTGTCTAA+TGG + chr1_3:20295772-20295791 Msa0108700:CDS 50.0%
AAGAAGCCCTAGTAATCCGG+TGG + chr1_3:20296029-20296048 Msa0108700:intron 50.0%
TGCTGCCTTAGCTGTGGATG+AGG + chr1_3:20295636-20295655 Msa0108700:CDS 55.0%
TGCCTTAGCTGTGGATGAGG+AGG + chr1_3:20295639-20295658 Msa0108700:CDS 55.0%
CACCTCCTCATCCACAGCTA+AGG - chr1_3:20295644-20295663 None:intergenic 55.0%
TGCTGCCTTAGCTGTGGATG+AGG + chr1_3:20295636-20295655 Msa0108700:CDS 55.0%
TGCCTTAGCTGTGGATGAGG+AGG + chr1_3:20295639-20295658 Msa0108700:CDS 55.0%
CACCTCCTCATCCACAGCTA+AGG - chr1_3:20295644-20295663 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 20294990 20296686 20294990 ID=Msa0108700;Name=Msa0108700
chr1_3 mRNA 20294990 20296686 20294990 ID=Msa0108700-mRNA-1;Parent=Msa0108700;Name=Msa0108700-mRNA-1;_AED=0.06;_eAED=0.06;_QI=213|0.66|0.75|1|1|1|4|216|101
chr1_3 exon 20294990 20295246 20294990 ID=Msa0108700-mRNA-1:exon:7326;Parent=Msa0108700-mRNA-1
chr1_3 exon 20295570 20295660 20295570 ID=Msa0108700-mRNA-1:exon:7327;Parent=Msa0108700-mRNA-1
chr1_3 exon 20295751 20295806 20295751 ID=Msa0108700-mRNA-1:exon:7328;Parent=Msa0108700-mRNA-1
chr1_3 exon 20296356 20296686 20296356 ID=Msa0108700-mRNA-1:exon:7329;Parent=Msa0108700-mRNA-1
chr1_3 five_prime_UTR 20294990 20295202 20294990 ID=Msa0108700-mRNA-1:five_prime_utr;Parent=Msa0108700-mRNA-1
chr1_3 CDS 20295203 20295246 20295203 ID=Msa0108700-mRNA-1:cds;Parent=Msa0108700-mRNA-1
chr1_3 CDS 20295570 20295660 20295570 ID=Msa0108700-mRNA-1:cds;Parent=Msa0108700-mRNA-1
chr1_3 CDS 20295751 20295806 20295751 ID=Msa0108700-mRNA-1:cds;Parent=Msa0108700-mRNA-1
chr1_3 CDS 20296356 20296470 20296356 ID=Msa0108700-mRNA-1:cds;Parent=Msa0108700-mRNA-1
chr1_3 three_prime_UTR 20296471 20296686 20296471 ID=Msa0108700-mRNA-1:three_prime_utr;Parent=Msa0108700-mRNA-1
Gene Sequence

>Msa0108700

ATGGATGCCACACCACACAACCAATCCCAATGGGATTTTACCAGATGGAATGACAATTGCAGTGACTTGGAAGTGGATTTTGGTTCTCAAGAGAATGCTTCTATTGTATATGCTGCCTTAGCTGTGGATGAGGAGTTGCAACCTGACAAAGTTAAACGACTCATGACGGTGTCTAATGGAAAACTATCAGTGCACTTTGAGGCAATAGAAGCCAGATTTCTTCGAGCATCATTCAGTGCTTTTGTAGATGTCCTTACTCTGGCAACCAAAACAATTGAAGAATTTGGTCAAGGAATGGAGCTGTGA
Protein sequence

>Msa0108700

MDATPHNQSQWDFTRWNDNCSDLEVDFGSQENASIVYAALAVDEELQPDKVKRLMTVSNGKLSVHFEAIEARFLRASFSAFVDVLTLATKTIEEFGQGMEL*