AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0122160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122160 MtrunA17_Chr1g0182901 93.056 288 9 1 1 288 1 277 0.0 548
Msa0122160 MtrunA17_Chr3g0115091 86.111 288 27 2 1 288 1 275 4.79e-169 469
Msa0122160 MtrunA17_Chr2g0277211 37.374 99 57 2 182 277 53 149 4.56e-13 67.0
Msa0122160 MtrunA17_Chr2g0298061 47.761 67 33 1 211 277 103 167 3.01e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122160 AT3G20970.1 78.995 219 35 1 68 286 72 279 2.23e-126 362
Msa0122160 AT1G51390.1 75.217 230 42 3 50 279 53 267 2.71e-123 353
Msa0122160 AT3G20970.2 78.261 161 24 1 68 228 72 221 2.88e-88 263
Msa0122160 AT5G49940.2 33.636 110 67 2 168 277 58 161 3.07e-13 67.4
Msa0122160 AT5G49940.1 46.269 67 34 1 211 277 97 161 1.19e-12 66.6
Msa0122160 AT4G25910.1 47.761 67 33 1 211 277 96 160 1.23e-12 66.6

Find 108 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATCTTTGCTGCTATTA+TGG 0.182790 1_3:+48504110 Msa0122160:CDS
CAAAGTTCAGTCTTCTGTTT+TGG 0.211936 1_3:-48503553 None:intergenic
TGCGATGATCAAGGAACTTT+TGG 0.229879 1_3:+48504418 Msa0122160:CDS
CTCCTTTGCCCGGTGGAAAA+TGG 0.230244 1_3:-48503778 None:intergenic
TAACAATAACAGAGTTGTTT+TGG 0.232889 1_3:+48503476 Msa0122160:five_prime_UTR
ATTATGGATTTCTACTCTTC+AGG 0.292022 1_3:+48504126 Msa0122160:CDS
TTCATCGCGGAACGAGGATT+AGG 0.297871 1_3:-48503895 None:intergenic
TCTATCTGCCTGGGCTACTA+TGG 0.303127 1_3:+48505664 Msa0122160:three_prime_UTR
TCCATGTTCAAATGGATGTA+TGG 0.310341 1_3:+48505812 Msa0122160:three_prime_UTR
CCATGACGCATCTTCCGATT+TGG 0.310465 1_3:-48504073 None:intergenic
ACTTGTATTGATATAAGATT+TGG 0.326652 1_3:+48505774 Msa0122160:three_prime_UTR
CAGCAAAGATTTCAGGCTTA+AGG 0.326720 1_3:-48504101 None:intergenic
ATGAAGGAAGCTTGAGGGTT+TGG 0.330225 1_3:+48503503 Msa0122160:five_prime_UTR
GTCTTTGGAAGCGGCAGCTT+GGG 0.330417 1_3:-48504169 None:intergenic
ACTGGCCGTGTCATGCAATA+AGG 0.341952 1_3:+48505969 Msa0122160:three_prime_UTR
AAATCAACCACGATGATATC+TGG 0.345328 1_3:-48505935 None:intergenic
ATAATAGCAGCAAAGATTTC+AGG 0.354714 1_3:-48504108 None:intergenic
AACATAAGAGACTCTGGATT+TGG 0.370612 1_3:-48503829 None:intergenic
GATTTGGAGTGGACTGTGTT+TGG 0.376256 1_3:-48503813 None:intergenic
AGGCATTATTACTTGCCTAT+TGG 0.384627 1_3:+48505734 Msa0122160:three_prime_UTR
ATCGTGGATTGCTGTGTCTT+TGG 0.387795 1_3:-48504184 None:intergenic
TCATCGCGGAACGAGGATTA+GGG 0.388776 1_3:-48503894 None:intergenic
ACTTGCCTATTGGCATTGTA+TGG 0.390828 1_3:+48505744 Msa0122160:three_prime_UTR
CAAAGGTGTGGAACAAGAAT+TGG 0.398435 1_3:+48505370 Msa0122160:CDS
TTCAGAGTCACCGAAGAACT+TGG 0.410747 1_3:-48504879 None:intergenic
GGTGGTACCCTCTATCTGCC+TGG 0.418536 1_3:+48505654 Msa0122160:three_prime_UTR
ATTGCATGACACGGCCAGTT+TGG 0.422105 1_3:-48505965 None:intergenic
AACAGAAGACTGAACTTTGA+AGG 0.425273 1_3:+48503557 Msa0122160:five_prime_UTR
TCTTCGGTGACTCTGAAATC+AGG 0.425848 1_3:+48504885 Msa0122160:CDS
TGGTCTTTCCTTCTGAATCA+AGG 0.425927 1_3:+48505695 Msa0122160:three_prime_UTR
TGTGATTAGTCTCTGATTTG+AGG 0.428418 1_3:-48503645 None:intergenic
GTGATTAGTCTCTGATTTGA+GGG 0.430671 1_3:-48503644 None:intergenic
ACCTATGCAACCTTTCTGTA+TGG 0.436964 1_3:-48505634 None:intergenic
TTGATTTCATACAGCCAAAC+TGG 0.444906 1_3:+48505951 Msa0122160:three_prime_UTR
GGTGGATGAAGGAAGCTTGA+GGG 0.447708 1_3:+48503498 Msa0122160:five_prime_UTR
ATGGATGTATGGGCATGTTA+AGG 0.449990 1_3:+48505823 Msa0122160:three_prime_UTR
TGTCTTTGGAAGCGGCAGCT+TGG 0.456320 1_3:-48504170 None:intergenic
GGGCTACTATGGATGGCTCT+TGG 0.458274 1_3:+48505675 Msa0122160:three_prime_UTR
GAAGCTTGAGGGTTTGGAGC+AGG 0.466202 1_3:+48503509 Msa0122160:five_prime_UTR
GGTGGGGACATTGTGTATTG+TGG 0.468819 1_3:+48504473 Msa0122160:CDS
TAGTGGCTGCCCAAGTTCTT+CGG 0.469327 1_3:+48504869 Msa0122160:CDS
TGCTATAGGTATTCTAGCGT+TGG 0.474248 1_3:+48505895 Msa0122160:three_prime_UTR
ATATTTAATAAGTTGTGAGT+TGG 0.479714 1_3:+48505526 Msa0122160:three_prime_UTR
ATAGTAGCCCAGGCAGATAG+AGG 0.481651 1_3:-48505662 None:intergenic
TTCAGTAACCTTGATTCAGA+AGG 0.492674 1_3:-48505703 None:intergenic
AGAGCCATCCATAGTAGCCC+AGG 0.495443 1_3:-48505672 None:intergenic
GGAACGAGGATTAGGGAAAT+CGG 0.495781 1_3:-48503887 None:intergenic
AGCAGCTTTAAGTGGACAAA+CGG 0.505572 1_3:+48505409 Msa0122160:CDS
AACCACGATGATATCTGGCT+TGG 0.514548 1_3:-48505930 None:intergenic
GTCCTCCATACAATGCCAAT+AGG 0.525615 1_3:-48505749 None:intergenic
TCTGCCTGGGCTACTATGGA+TGG 0.529289 1_3:+48505668 Msa0122160:three_prime_UTR
CCATGTTCAAATGGATGTAT+GGG 0.530038 1_3:+48505813 Msa0122160:three_prime_UTR
GAAGAAGAAGCAGCTTTAAG+TGG 0.530262 1_3:+48505401 Msa0122160:CDS
TCGTATCCGACCAACTGTAC+AGG 0.532621 1_3:+48504445 Msa0122160:CDS
CAAATCGGAAGATGCGTCAT+GGG 0.534660 1_3:+48504074 Msa0122160:CDS
GTGGTACCCTCTATCTGCCT+GGG 0.535551 1_3:+48505655 Msa0122160:three_prime_UTR
AGATGGAAATGGTGAGGCGG+AGG 0.537192 1_3:-48503755 None:intergenic
GGATGAAACATAAGAGACTC+TGG 0.539372 1_3:-48503835 None:intergenic
ACATCCATGACAGGTTTACC+AGG 0.541119 1_3:-48503856 None:intergenic
TGAAACAGTTGCGATGATCA+AGG 0.541980 1_3:+48504409 Msa0122160:CDS
GATTGCTGTGTCTTTGGAAG+CGG 0.547638 1_3:-48504178 None:intergenic
CCAAATCGGAAGATGCGTCA+TGG 0.548834 1_3:+48504073 Msa0122160:CDS
GGGTGGATGAAGGAAGCTTG+AGG 0.549678 1_3:+48503497 Msa0122160:five_prime_UTR
TTGCTGCATTTACAGGTCAA+AGG 0.551548 1_3:+48505353 Msa0122160:intron
AACGCTAGAATACCTATAGC+AGG 0.556944 1_3:-48505893 None:intergenic
CGACCAACTGTACAGGATGA+TGG 0.562611 1_3:+48504452 Msa0122160:CDS
GTGGAAAATGGAGATGGAAA+TGG 0.563081 1_3:-48503766 None:intergenic
AAACATACTCCTCCTTTGCC+CGG 0.564888 1_3:-48503788 None:intergenic
TCAGAGTCACCGAAGAACTT+GGG 0.566646 1_3:-48504878 None:intergenic
GCATTGCGGTTGTAGTTGCA+AGG 0.571191 1_3:-48503721 None:intergenic
CCCATACATCCATTTGAACA+TGG 0.572234 1_3:-48505813 None:intergenic
GAGTCTCTTATGTTTCATCC+TGG 0.574002 1_3:+48503838 Msa0122160:CDS
TGCCCGGTGGAAAATGGAGA+TGG 0.578393 1_3:-48503772 None:intergenic
CCACCATCATCCTGTACAGT+TGG 0.579401 1_3:-48504455 None:intergenic
ATTACAACAACAGTGCATGA+TGG 0.581684 1_3:+48505472 Msa0122160:three_prime_UTR
AACTGTACAGGATGATGGTG+GGG 0.584854 1_3:+48504457 Msa0122160:CDS
GGTAAACCTGTCATGGATGT+TGG 0.587174 1_3:+48503859 Msa0122160:CDS
GACTGAACTTTGAAGGATGA+CGG 0.591664 1_3:+48503564 Msa0122160:exon
ATACAGAAAGGTTGCATAGG+TGG 0.591872 1_3:+48505636 Msa0122160:three_prime_UTR
TCATCCTGGTAAACCTGTCA+TGG 0.592000 1_3:+48503852 Msa0122160:CDS
GGAGAATTCATCGCGGAACG+AGG 0.593137 1_3:-48503901 None:intergenic
CAACTGTACAGGATGATGGT+GGG 0.594080 1_3:+48504456 Msa0122160:CDS
ATCGTCAAACTCAAAATGCA+AGG 0.595778 1_3:+48504840 Msa0122160:CDS
GCTTGAGGGTTTGGAGCAGG+AGG 0.600028 1_3:+48503512 Msa0122160:five_prime_UTR
TGCCTATTGGCATTGTATGG+AGG 0.602555 1_3:+48505747 Msa0122160:three_prime_UTR
CCAACTGTACAGGATGATGG+TGG 0.609164 1_3:+48504455 Msa0122160:CDS
GCGCTACCAACATCCATGAC+AGG 0.609183 1_3:-48503865 None:intergenic
AACACGGTGTCCATACAGAA+AGG 0.610138 1_3:+48505624 Msa0122160:three_prime_UTR
TAGTAGCCCAGGCAGATAGA+GGG 0.611396 1_3:-48505661 None:intergenic
CATCATCCTGTACAGTTGGT+CGG 0.612141 1_3:-48504451 None:intergenic
TTTGAAGGATGACGGTGGTG+AGG 0.624455 1_3:+48503572 Msa0122160:exon
GCTTGGGAGTCTAGAAAGAG+TGG 0.628813 1_3:-48504153 None:intergenic
GCATTTACAGGTCAAAGGTG+TGG 0.632082 1_3:+48505358 Msa0122160:intron
TGGAGATGGAAATGGTGAGG+CGG 0.634856 1_3:-48503758 None:intergenic
AAGAGACTCTGGATTTGGAG+TGG 0.639473 1_3:-48503824 None:intergenic
TCCATACAGAAAGGTTGCAT+AGG 0.641462 1_3:+48505633 Msa0122160:three_prime_UTR
TAGTTGCAAGGTTGAAACGA+TGG 0.642224 1_3:-48503709 None:intergenic
AAAATGCAAGGAGCATGTAG+TGG 0.643660 1_3:+48504852 Msa0122160:CDS
CATACTCCTCCTTTGCCCGG+TGG 0.656092 1_3:-48503785 None:intergenic
AAAATCCTTATTGCATGACA+CGG 0.661294 1_3:-48505974 None:intergenic
TTAGGAAAACGATTACGACT+AGG 0.664261 1_3:+48503610 Msa0122160:CDS
AAATGGAGATGGAAATGGTG+AGG 0.665109 1_3:-48503761 None:intergenic
GGAACAAGAATTGGATGCTG+AGG 0.670849 1_3:+48505379 Msa0122160:CDS
GCTGATGCATTATGTACCTG+AGG 0.715793 1_3:+48504920 Msa0122160:CDS
TATGAAGGTAGTTCATAACA+CGG 0.725666 1_3:+48505608 Msa0122160:intron
CTCCAAGCCAGATATCATCG+TGG 0.760449 1_3:+48505928 Msa0122160:three_prime_UTR
AGCAAGAGGAGAATTCATCG+CGG 0.788482 1_3:-48503908 None:intergenic
TGAACTTTGAAGGATGACGG+TGG 0.812467 1_3:+48503567 Msa0122160:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGAAATTTAAGTAAA+AGG - chr1_3:48504267-48504286 None:intergenic 10.0%
!!! TTTTACTTAAATTTCATAAA+AGG + chr1_3:48504266-48504285 Msa0122160:intron 10.0%
!!! TAATTTTATTTCAAGGAATT+TGG + chr1_3:48504241-48504260 Msa0122160:intron 15.0%
!! ATATAAAAAACAAGTAAAAG+AGG - chr1_3:48504795-48504814 None:intergenic 15.0%
!!! TTATTTAAAGCAGTATTAAA+AGG + chr1_3:48505266-48505285 Msa0122160:intron 15.0%
!! AAAACAAAAACAAAAACAAA+AGG + chr1_3:48505846-48505865 Msa0122160:three_prime_UTR 15.0%
!!! AAATCAATTTTTGCTATTGA+TGG + chr1_3:48503931-48503950 Msa0122160:CDS 20.0%
!! TAAAAAAACAATCAGAAAAC+AGG - chr1_3:48504001-48504020 None:intergenic 20.0%
!!! ATTGTTTTTTTAATTGTTGC+AGG + chr1_3:48504009-48504028 Msa0122160:intron 20.0%
!!! ATTGCTGTAATTTTATTTCA+AGG + chr1_3:48504234-48504253 Msa0122160:intron 20.0%
!!! GAACTTTATCTCATTATTTT+AGG + chr1_3:48504624-48504643 Msa0122160:intron 20.0%
!! ATATAAATGGAAACAACAAA+TGG - chr1_3:48504982-48505001 None:intergenic 20.0%
!!! GTTGTTTCCATTTATATTTA+TGG + chr1_3:48504985-48505004 Msa0122160:intron 20.0%
!! TCAATCACCATAAATATAAA+TGG - chr1_3:48504995-48505014 None:intergenic 20.0%
!! AGCATATCTTATAAGTTAAT+TGG - chr1_3:48505127-48505146 None:intergenic 20.0%
!! AAATAGAAAACCTAATTCAA+TGG - chr1_3:48505159-48505178 None:intergenic 20.0%
!! TTTCTATTTATCTTACTTAC+AGG + chr1_3:48505170-48505189 Msa0122160:intron 20.0%
!! GTTGAACAATTCATATAAAT+TGG - chr1_3:48505299-48505318 None:intergenic 20.0%
!!! CTCATATTTTTCTATAATTG+CGG + chr1_3:48505442-48505461 Msa0122160:three_prime_UTR 20.0%
!! ATATTTAATAAGTTGTGAGT+TGG + chr1_3:48505526-48505545 Msa0122160:three_prime_UTR 20.0%
!!! ACTTGTATTGATATAAGATT+TGG + chr1_3:48505774-48505793 Msa0122160:three_prime_UTR 20.0%
!!! CTATACCGTATTATTTTTTT+GGG + chr1_3:48506003-48506022 Msa0122160:three_prime_UTR 20.0%
!!! TATACCGTATTATTTTTTTG+GGG + chr1_3:48506004-48506023 Msa0122160:three_prime_UTR 20.0%
!! TAACAATAACAGAGTTGTTT+TGG + chr1_3:48503476-48503495 Msa0122160:five_prime_UTR 25.0%
!!! AACAATAACAGAGTTGTTTT+GGG + chr1_3:48503477-48503496 Msa0122160:five_prime_UTR 25.0%
! AATAAAAGAAATGAAAGGAG+TGG - chr1_3:48503974-48503993 None:intergenic 25.0%
! GCACAAATAAAAGAAATGAA+AGG - chr1_3:48503979-48503998 None:intergenic 25.0%
! AGAGTATAGATTATTCATTG+AGG - chr1_3:48504554-48504573 None:intergenic 25.0%
!! GGAAATTTGCTCATTTAATT+TGG - chr1_3:48504725-48504744 None:intergenic 25.0%
!!! CATTCTTAAGTTGATTTTGA+GGG + chr1_3:48505076-48505095 Msa0122160:intron 25.0%
! ATTTATCTTACTTACAGGAT+TGG + chr1_3:48505175-48505194 Msa0122160:intron 25.0%
! AAAAACAAAAACAAAAGGCA+TGG + chr1_3:48505851-48505870 Msa0122160:three_prime_UTR 25.0%
!!! TTGATTTTTTTTCCTGCTAT+AGG + chr1_3:48505881-48505900 Msa0122160:three_prime_UTR 25.0%
!!! GCTATACCGTATTATTTTTT+TGG + chr1_3:48506002-48506021 Msa0122160:three_prime_UTR 25.0%
! TTCACCCCAAAAAAATAATA+CGG - chr1_3:48506011-48506030 None:intergenic 25.0%
! GCAAAAATTGATTTAGCAAG+AGG - chr1_3:48503925-48503944 None:intergenic 30.0%
! TTTTGTCACTGTTACCAAAT+CGG + chr1_3:48504059-48504078 Msa0122160:CDS 30.0%
ATAATAGCAGCAAAGATTTC+AGG - chr1_3:48504111-48504130 None:intergenic 30.0%
TGAAATCTTTGCTGCTATTA+TGG + chr1_3:48504110-48504129 Msa0122160:CDS 30.0%
ATTATGGATTTCTACTCTTC+AGG + chr1_3:48504126-48504145 Msa0122160:CDS 30.0%
AATGAGAAAAACTTACATCG+TGG - chr1_3:48504203-48504222 None:intergenic 30.0%
!!! ATTCAGTTTTGAGATCTTGA+AGG - chr1_3:48504356-48504375 None:intergenic 30.0%
TTCAAGATCTCAAAACTGAA+TGG + chr1_3:48504355-48504374 Msa0122160:intron 30.0%
!! TAAGTTTCCCCTTGTTATTT+GGG + chr1_3:48504506-48504525 Msa0122160:intron 30.0%
ATTGAAACCCAAATAACAAG+GGG - chr1_3:48504516-48504535 None:intergenic 30.0%
CATTGAAACCCAAATAACAA+GGG - chr1_3:48504517-48504536 None:intergenic 30.0%
ACATTGAAACCCAAATAACA+AGG - chr1_3:48504518-48504537 None:intergenic 30.0%
TGTCAACAACAATATTGTCT+TGG - chr1_3:48504746-48504765 None:intergenic 30.0%
!! GTTTGTTTTCTCTAGAGATA+CGG + chr1_3:48504815-48504834 Msa0122160:intron 30.0%
!! TTTGTTTTCTCTAGAGATAC+GGG + chr1_3:48504816-48504835 Msa0122160:intron 30.0%
TACAAATGCTTCATTACAGA+AGG - chr1_3:48505044-48505063 None:intergenic 30.0%
AATCAACTTAAGAATGCCAT+AGG - chr1_3:48505072-48505091 None:intergenic 30.0%
!!! GCATTCTTAAGTTGATTTTG+AGG + chr1_3:48505075-48505094 Msa0122160:intron 30.0%
GCATATTACAAGGATAAACT+TGG + chr1_3:48505098-48505117 Msa0122160:intron 30.0%
TAGAAAACCTAATTCAATGG+AGG - chr1_3:48505156-48505175 None:intergenic 30.0%
TATGAAGGTAGTTCATAACA+CGG + chr1_3:48505608-48505627 Msa0122160:intron 30.0%
AAAATCCTTATTGCATGACA+CGG - chr1_3:48505977-48505996 None:intergenic 30.0%
!!! AATAACAGAGTTGTTTTGGG+TGG + chr1_3:48503480-48503499 Msa0122160:five_prime_UTR 35.0%
! CAAAGTTCAGTCTTCTGTTT+TGG - chr1_3:48503556-48503575 None:intergenic 35.0%
AACAGAAGACTGAACTTTGA+AGG + chr1_3:48503557-48503576 Msa0122160:five_prime_UTR 35.0%
! GTCGTAATCGTTTTCCTAAT+CGG - chr1_3:48503609-48503628 None:intergenic 35.0%
TTAGGAAAACGATTACGACT+AGG + chr1_3:48503610-48503629 Msa0122160:CDS 35.0%
!! GTGATTAGTCTCTGATTTGA+GGG - chr1_3:48503647-48503666 None:intergenic 35.0%
!! TGTGATTAGTCTCTGATTTG+AGG - chr1_3:48503648-48503667 None:intergenic 35.0%
! TACAAATTGTTTTCGCTGTC+TGG + chr1_3:48503678-48503697 Msa0122160:CDS 35.0%
! ACAAATTGTTTTCGCTGTCT+GGG + chr1_3:48503679-48503698 Msa0122160:CDS 35.0%
!! AGGAAGAGAAAAAAGCATTG+CGG - chr1_3:48503738-48503757 None:intergenic 35.0%
AACATAAGAGACTCTGGATT+TGG - chr1_3:48503832-48503851 None:intergenic 35.0%
!! GTAAGTTTCCCCTTGTTATT+TGG + chr1_3:48504505-48504524 Msa0122160:intron 35.0%
!! TTGTTTTCTCTAGAGATACG+GGG + chr1_3:48504817-48504836 Msa0122160:intron 35.0%
ATCGTCAAACTCAAAATGCA+AGG + chr1_3:48504840-48504859 Msa0122160:CDS 35.0%
!! TAATGCAAACTTGTTACCTC+AGG - chr1_3:48504939-48504958 None:intergenic 35.0%
TACAGAAGGATGATAAGTTG+AGG - chr1_3:48505030-48505049 None:intergenic 35.0%
AAGCATTTGTACCTTTCCTA+TGG + chr1_3:48505053-48505072 Msa0122160:intron 35.0%
ACTTAAGAATGCCATAGGAA+AGG - chr1_3:48505067-48505086 None:intergenic 35.0%
!! GATTTTGAGGGCATATTACA+AGG + chr1_3:48505088-48505107 Msa0122160:intron 35.0%
TAGTTGTCCTCCATTGAATT+AGG + chr1_3:48505146-48505165 Msa0122160:intron 35.0%
ATTACAACAACAGTGCATGA+TGG + chr1_3:48505472-48505491 Msa0122160:three_prime_UTR 35.0%
ATCTCACTGGAATAGTATGA+AGG + chr1_3:48505593-48505612 Msa0122160:intron 35.0%
TTCAGTAACCTTGATTCAGA+AGG - chr1_3:48505706-48505725 None:intergenic 35.0%
AGGCATTATTACTTGCCTAT+TGG + chr1_3:48505734-48505753 Msa0122160:three_prime_UTR 35.0%
! TGCTTTTCTCCATGTTCAAA+TGG + chr1_3:48505804-48505823 Msa0122160:three_prime_UTR 35.0%
TCCATGTTCAAATGGATGTA+TGG + chr1_3:48505812-48505831 Msa0122160:three_prime_UTR 35.0%
CCATGTTCAAATGGATGTAT+GGG + chr1_3:48505813-48505832 Msa0122160:three_prime_UTR 35.0%
AAATCAACCACGATGATATC+TGG - chr1_3:48505938-48505957 None:intergenic 35.0%
TTGATTTCATACAGCCAAAC+TGG + chr1_3:48505951-48505970 Msa0122160:three_prime_UTR 35.0%
! GACTGAACTTTGAAGGATGA+CGG + chr1_3:48503564-48503583 Msa0122160:exon 40.0%
TAGTTGCAAGGTTGAAACGA+TGG - chr1_3:48503712-48503731 None:intergenic 40.0%
AAATGGAGATGGAAATGGTG+AGG - chr1_3:48503764-48503783 None:intergenic 40.0%
GTGGAAAATGGAGATGGAAA+TGG - chr1_3:48503769-48503788 None:intergenic 40.0%
! TTTCCATCTCCATTTTCCAC+CGG + chr1_3:48503769-48503788 Msa0122160:CDS 40.0%
GGATGAAACATAAGAGACTC+TGG - chr1_3:48503838-48503857 None:intergenic 40.0%
GAGTCTCTTATGTTTCATCC+TGG + chr1_3:48503838-48503857 Msa0122160:CDS 40.0%
AAATGAAAGGAGTGGAGAGA+GGG - chr1_3:48503966-48503985 None:intergenic 40.0%
! GGAATCACTCGTGTTTTCTT+TGG + chr1_3:48504030-48504049 Msa0122160:intron 40.0%
CAGCAAAGATTTCAGGCTTA+AGG - chr1_3:48504104-48504123 None:intergenic 40.0%
ATGCTTACCTGTTTGCATAC+AGG + chr1_3:48504379-48504398 Msa0122160:intron 40.0%
GAATCATCCTGTATGCAAAC+AGG - chr1_3:48504389-48504408 None:intergenic 40.0%
TGAAACAGTTGCGATGATCA+AGG + chr1_3:48504409-48504428 Msa0122160:CDS 40.0%
! TGCGATGATCAAGGAACTTT+TGG + chr1_3:48504418-48504437 Msa0122160:CDS 40.0%
GCAAAGGTAGAATGGAACTT+TGG - chr1_3:48504593-48504612 None:intergenic 40.0%
AGATAAAGTTCAACCCACCT+AGG - chr1_3:48504615-48504634 None:intergenic 40.0%
! AAAATGCAAGGAGCATGTAG+TGG + chr1_3:48504852-48504871 Msa0122160:CDS 40.0%
GTAAATGCAGCAAGCAACAT+TGG - chr1_3:48505346-48505365 None:intergenic 40.0%
! ATGTTGCTTGCTGCATTTAC+AGG + chr1_3:48505346-48505365 Msa0122160:intron 40.0%
TTGCTGCATTTACAGGTCAA+AGG + chr1_3:48505353-48505372 Msa0122160:intron 40.0%
! CAAAGGTGTGGAACAAGAAT+TGG + chr1_3:48505370-48505389 Msa0122160:CDS 40.0%
!! GAAGAAGAAGCAGCTTTAAG+TGG + chr1_3:48505401-48505420 Msa0122160:CDS 40.0%
!! AGCAGCTTTAAGTGGACAAA+CGG + chr1_3:48505409-48505428 Msa0122160:CDS 40.0%
TCCATACAGAAAGGTTGCAT+AGG + chr1_3:48505633-48505652 Msa0122160:three_prime_UTR 40.0%
ACCTATGCAACCTTTCTGTA+TGG - chr1_3:48505637-48505656 None:intergenic 40.0%
ATACAGAAAGGTTGCATAGG+TGG + chr1_3:48505636-48505655 Msa0122160:three_prime_UTR 40.0%
TGGTCTTTCCTTCTGAATCA+AGG + chr1_3:48505695-48505714 Msa0122160:three_prime_UTR 40.0%
! AAGGTTACTGAAAAAGTGCG+AGG + chr1_3:48505714-48505733 Msa0122160:three_prime_UTR 40.0%
ACTTGCCTATTGGCATTGTA+TGG + chr1_3:48505744-48505763 Msa0122160:three_prime_UTR 40.0%
CCCATACATCCATTTGAACA+TGG - chr1_3:48505816-48505835 None:intergenic 40.0%
ATGGATGTATGGGCATGTTA+AGG + chr1_3:48505823-48505842 Msa0122160:three_prime_UTR 40.0%
AACGCTAGAATACCTATAGC+AGG - chr1_3:48505896-48505915 None:intergenic 40.0%
TGCTATAGGTATTCTAGCGT+TGG + chr1_3:48505895-48505914 Msa0122160:three_prime_UTR 40.0%
!!! GAGTTGTTTTGGGTGGATGA+AGG + chr1_3:48503487-48503506 Msa0122160:five_prime_UTR 45.0%
ATGAAGGAAGCTTGAGGGTT+TGG + chr1_3:48503503-48503522 Msa0122160:five_prime_UTR 45.0%
! TGAACTTTGAAGGATGACGG+TGG + chr1_3:48503567-48503586 Msa0122160:exon 45.0%
! AGGCGTACTGTTTTCCGATT+AGG + chr1_3:48503592-48503611 Msa0122160:CDS 45.0%
! TTCCATCTCCATTTTCCACC+GGG + chr1_3:48503770-48503789 Msa0122160:CDS 45.0%
AAACATACTCCTCCTTTGCC+CGG - chr1_3:48503791-48503810 None:intergenic 45.0%
!! GATTTGGAGTGGACTGTGTT+TGG - chr1_3:48503816-48503835 None:intergenic 45.0%
AAGAGACTCTGGATTTGGAG+TGG - chr1_3:48503827-48503846 None:intergenic 45.0%
TCATCCTGGTAAACCTGTCA+TGG + chr1_3:48503852-48503871 Msa0122160:CDS 45.0%
ACATCCATGACAGGTTTACC+AGG - chr1_3:48503859-48503878 None:intergenic 45.0%
GGTAAACCTGTCATGGATGT+TGG + chr1_3:48503859-48503878 Msa0122160:CDS 45.0%
GGAACGAGGATTAGGGAAAT+CGG - chr1_3:48503890-48503909 None:intergenic 45.0%
AGCAAGAGGAGAATTCATCG+CGG - chr1_3:48503911-48503930 None:intergenic 45.0%
AATGAAAGGAGTGGAGAGAG+GGG - chr1_3:48503965-48503984 None:intergenic 45.0%
GAAATGAAAGGAGTGGAGAG+AGG - chr1_3:48503967-48503986 None:intergenic 45.0%
CAAATCGGAAGATGCGTCAT+GGG + chr1_3:48504074-48504093 Msa0122160:CDS 45.0%
! GATTGCTGTGTCTTTGGAAG+CGG - chr1_3:48504181-48504200 None:intergenic 45.0%
! ATCGTGGATTGCTGTGTCTT+TGG - chr1_3:48504187-48504206 None:intergenic 45.0%
CAATTCACGATCCAAGTGCT+CGG + chr1_3:48504302-48504321 Msa0122160:intron 45.0%
AATTCACGATCCAAGTGCTC+GGG + chr1_3:48504303-48504322 Msa0122160:intron 45.0%
! CGGGAGTATGAGATTTGTCT+AGG + chr1_3:48504322-48504341 Msa0122160:intron 45.0%
CATCATCCTGTACAGTTGGT+CGG - chr1_3:48504454-48504473 None:intergenic 45.0%
! CAACTGTACAGGATGATGGT+GGG + chr1_3:48504456-48504475 Msa0122160:CDS 45.0%
! AACTGTACAGGATGATGGTG+GGG + chr1_3:48504457-48504476 Msa0122160:CDS 45.0%
GTTCCATTCTACCTTTGCCT+AGG + chr1_3:48504595-48504614 Msa0122160:intron 45.0%
CATTCTACCTTTGCCTAGGT+GGG + chr1_3:48504599-48504618 Msa0122160:intron 45.0%
TCAGAGTCACCGAAGAACTT+GGG - chr1_3:48504881-48504900 None:intergenic 45.0%
TTCAGAGTCACCGAAGAACT+TGG - chr1_3:48504882-48504901 None:intergenic 45.0%
!! TCTTCGGTGACTCTGAAATC+AGG + chr1_3:48504885-48504904 Msa0122160:CDS 45.0%
GCTGATGCATTATGTACCTG+AGG + chr1_3:48504920-48504939 Msa0122160:CDS 45.0%
GCATTTACAGGTCAAAGGTG+TGG + chr1_3:48505358-48505377 Msa0122160:intron 45.0%
GGAACAAGAATTGGATGCTG+AGG + chr1_3:48505379-48505398 Msa0122160:CDS 45.0%
CTGAGCTAGTTAGATCTCAC+TGG + chr1_3:48505580-48505599 Msa0122160:intron 45.0%
AACACGGTGTCCATACAGAA+AGG + chr1_3:48505624-48505643 Msa0122160:three_prime_UTR 45.0%
TGCCTATTGGCATTGTATGG+AGG + chr1_3:48505747-48505766 Msa0122160:three_prime_UTR 45.0%
GTCCTCCATACAATGCCAAT+AGG - chr1_3:48505752-48505771 None:intergenic 45.0%
AACCACGATGATATCTGGCT+TGG - chr1_3:48505933-48505952 None:intergenic 45.0%
GGTGGATGAAGGAAGCTTGA+GGG + chr1_3:48503498-48503517 Msa0122160:five_prime_UTR 50.0%
! TTTGAAGGATGACGGTGGTG+AGG + chr1_3:48503572-48503591 Msa0122160:exon 50.0%
GCATTGCGGTTGTAGTTGCA+AGG - chr1_3:48503724-48503743 None:intergenic 50.0%
TGGAGATGGAAATGGTGAGG+CGG - chr1_3:48503761-48503780 None:intergenic 50.0%
TCATCGCGGAACGAGGATTA+GGG - chr1_3:48503897-48503916 None:intergenic 50.0%
TTCATCGCGGAACGAGGATT+AGG - chr1_3:48503898-48503917 None:intergenic 50.0%
ATGAAAGGAGTGGAGAGAGG+GGG - chr1_3:48503964-48503983 None:intergenic 50.0%
CCATGACGCATCTTCCGATT+TGG - chr1_3:48504076-48504095 None:intergenic 50.0%
CCAAATCGGAAGATGCGTCA+TGG + chr1_3:48504073-48504092 Msa0122160:CDS 50.0%
GCTTGGGAGTCTAGAAAGAG+TGG - chr1_3:48504156-48504175 None:intergenic 50.0%
CGAGCACTTGGATCGTGAAT+TGG - chr1_3:48504304-48504323 None:intergenic 50.0%
ATCTCATACTCCCGAGCACT+TGG - chr1_3:48504316-48504335 None:intergenic 50.0%
TCGTATCCGACCAACTGTAC+AGG + chr1_3:48504445-48504464 Msa0122160:CDS 50.0%
CGACCAACTGTACAGGATGA+TGG + chr1_3:48504452-48504471 Msa0122160:CDS 50.0%
CCACCATCATCCTGTACAGT+TGG - chr1_3:48504458-48504477 None:intergenic 50.0%
! CCAACTGTACAGGATGATGG+TGG + chr1_3:48504455-48504474 Msa0122160:CDS 50.0%
GGTGGGGACATTGTGTATTG+TGG + chr1_3:48504473-48504492 Msa0122160:CDS 50.0%
CCACCTAGGCAAAGGTAGAA+TGG - chr1_3:48504601-48504620 None:intergenic 50.0%
CCATTCTACCTTTGCCTAGG+TGG + chr1_3:48504598-48504617 Msa0122160:intron 50.0%
AGTTCAACCCACCTAGGCAA+AGG - chr1_3:48504609-48504628 None:intergenic 50.0%
TAGTGGCTGCCCAAGTTCTT+CGG + chr1_3:48504869-48504888 Msa0122160:CDS 50.0%
TAGTAGCCCAGGCAGATAGA+GGG - chr1_3:48505664-48505683 None:intergenic 50.0%
ATAGTAGCCCAGGCAGATAG+AGG - chr1_3:48505665-48505684 None:intergenic 50.0%
TCTATCTGCCTGGGCTACTA+TGG + chr1_3:48505664-48505683 Msa0122160:three_prime_UTR 50.0%
CTCCAAGCCAGATATCATCG+TGG + chr1_3:48505928-48505947 Msa0122160:three_prime_UTR 50.0%
ATTGCATGACACGGCCAGTT+TGG - chr1_3:48505968-48505987 None:intergenic 50.0%
ACTGGCCGTGTCATGCAATA+AGG + chr1_3:48505969-48505988 Msa0122160:three_prime_UTR 50.0%
GGGTGGATGAAGGAAGCTTG+AGG + chr1_3:48503497-48503516 Msa0122160:five_prime_UTR 55.0%
! GAAGCTTGAGGGTTTGGAGC+AGG + chr1_3:48503509-48503528 Msa0122160:five_prime_UTR 55.0%
AGATGGAAATGGTGAGGCGG+AGG - chr1_3:48503758-48503777 None:intergenic 55.0%
TGCCCGGTGGAAAATGGAGA+TGG - chr1_3:48503775-48503794 None:intergenic 55.0%
! CTCCATTTTCCACCGGGCAA+AGG + chr1_3:48503776-48503795 Msa0122160:CDS 55.0%
CTCCTTTGCCCGGTGGAAAA+TGG - chr1_3:48503781-48503800 None:intergenic 55.0%
! CATTTTCCACCGGGCAAAGG+AGG + chr1_3:48503779-48503798 Msa0122160:CDS 55.0%
GCGCTACCAACATCCATGAC+AGG - chr1_3:48503868-48503887 None:intergenic 55.0%
GGAGAATTCATCGCGGAACG+AGG - chr1_3:48503904-48503923 None:intergenic 55.0%
TGAAAGGAGTGGAGAGAGGG+GGG - chr1_3:48503963-48503982 None:intergenic 55.0%
! GTCTTTGGAAGCGGCAGCTT+GGG - chr1_3:48504172-48504191 None:intergenic 55.0%
! TGTCTTTGGAAGCGGCAGCT+TGG - chr1_3:48504173-48504192 None:intergenic 55.0%
GTGGTACCCTCTATCTGCCT+GGG + chr1_3:48505655-48505674 Msa0122160:three_prime_UTR 55.0%
TCTGCCTGGGCTACTATGGA+TGG + chr1_3:48505668-48505687 Msa0122160:three_prime_UTR 55.0%
AGAGCCATCCATAGTAGCCC+AGG - chr1_3:48505675-48505694 None:intergenic 55.0%
GGGCTACTATGGATGGCTCT+TGG + chr1_3:48505675-48505694 Msa0122160:three_prime_UTR 55.0%
! GCTTGAGGGTTTGGAGCAGG+AGG + chr1_3:48503512-48503531 Msa0122160:five_prime_UTR 60.0%
CATACTCCTCCTTTGCCCGG+TGG - chr1_3:48503788-48503807 None:intergenic 60.0%
GAAAGGAGTGGAGAGAGGGG+GGG - chr1_3:48503962-48503981 None:intergenic 60.0%
GGTGGTACCCTCTATCTGCC+TGG + chr1_3:48505654-48505673 Msa0122160:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 48503457 48506040 48503457 ID=Msa0122160;Name=Msa0122160
chr1_3 mRNA 48503457 48506040 48503457 ID=Msa0122160-mRNA-1;Parent=Msa0122160;Name=Msa0122160-mRNA-1;_AED=0.16;_eAED=0.16;_QI=123|0.8|0.83|1|0.8|0.66|6|578|288
chr1_3 exon 48503457 48503952 48503457 ID=Msa0122160-mRNA-1:exon:14655;Parent=Msa0122160-mRNA-1
chr1_3 exon 48504031 48504239 48504031 ID=Msa0122160-mRNA-1:exon:14656;Parent=Msa0122160-mRNA-1
chr1_3 exon 48504401 48504504 48504401 ID=Msa0122160-mRNA-1:exon:14657;Parent=Msa0122160-mRNA-1
chr1_3 exon 48504830 48504941 48504830 ID=Msa0122160-mRNA-1:exon:14658;Parent=Msa0122160-mRNA-1
chr1_3 exon 48505368 48505592 48505368 ID=Msa0122160-mRNA-1:exon:14659;Parent=Msa0122160-mRNA-1
chr1_3 exon 48505619 48506040 48505619 ID=Msa0122160-mRNA-1:exon:14660;Parent=Msa0122160-mRNA-1
chr1_3 five_prime_UTR 48503457 48503579 48503457 ID=Msa0122160-mRNA-1:five_prime_utr;Parent=Msa0122160-mRNA-1
chr1_3 CDS 48503580 48503952 48503580 ID=Msa0122160-mRNA-1:cds;Parent=Msa0122160-mRNA-1
chr1_3 CDS 48504031 48504239 48504031 ID=Msa0122160-mRNA-1:cds;Parent=Msa0122160-mRNA-1
chr1_3 CDS 48504401 48504504 48504401 ID=Msa0122160-mRNA-1:cds;Parent=Msa0122160-mRNA-1
chr1_3 CDS 48504830 48504941 48504830 ID=Msa0122160-mRNA-1:cds;Parent=Msa0122160-mRNA-1
chr1_3 CDS 48505368 48505436 48505368 ID=Msa0122160-mRNA-1:cds;Parent=Msa0122160-mRNA-1
chr1_3 three_prime_UTR 48505437 48505592 48505437 ID=Msa0122160-mRNA-1:three_prime_utr;Parent=Msa0122160-mRNA-1
chr1_3 three_prime_UTR 48505619 48506040 48505619 ID=Msa0122160-mRNA-1:three_prime_utr;Parent=Msa0122160-mRNA-1
Gene Sequence

>Msa0122160

ATGACGGTGGTGAGGCGTACTGTTTTCCGATTAGGAAAACGATTACGACTAGGACTCTTCAATTCCCTCAAATCAGAGACTAATCACAATGATCTAAATACAAATTGTTTTCGCTGTCTGGGTGCAACGCCATCGTTTCAACCTTGCAACTACAACCGCAATGCTTTTTTCTCTTCCTCCGCCTCACCATTTCCATCTCCATTTTCCACCGGGCAAAGGAGGAGTATGTTTATCCAAACACAGTCCACTCCAAATCCAGAGTCTCTTATGTTTCATCCTGGTAAACCTGTCATGGATGTTGGTAGCGCCGATTTCCCTAATCCTCGTTCCGCGATGAATTCTCCTCTTGCTAAATCAATTTTTGCTATTGATGGAATCACTCGTGTTTTCTTTGGATCTGATTTTGTCACTGTTACCAAATCGGAAGATGCGTCATGGGAATTCCTTAAGCCTGAAATCTTTGCTGCTATTATGGATTTCTACTCTTCAGGTGAACCACTCTTTCTAGACTCCCAAGCTGCCGCTTCCAAAGACACAGCAATCCACGATGTAAGTTTTTCTCATTTCTATGCTGATATTGCTGATGATTCTGAAACAGTTGCGATGATCAAGGAACTTTTGGAGACTCGTATCCGACCAACTGTACAGGATGATGGTGGGGACATTGTGTATTGTGGTTTTGATTCAGATACGGGGATCGTCAAACTCAAAATGCAAGGAGCATGTAGTGGCTGCCCAAGTTCTTCGGTGACTCTGAAATCAGGCATTGAGAATATGCTGATGCATTATGTACCTGAGGTCAAAGGTGTGGAACAAGAATTGGATGCTGAGGAAGAAGAAGCAGCTTTAAGTGGACAAACGGAGTAG
Protein sequence

>Msa0122160

MTVVRRTVFRLGKRLRLGLFNSLKSETNHNDLNTNCFRCLGATPSFQPCNYNRNAFFSSSASPFPSPFSTGQRRSMFIQTQSTPNPESLMFHPGKPVMDVGSADFPNPRSAMNSPLAKSIFAIDGITRVFFGSDFVTVTKSEDASWEFLKPEIFAAIMDFYSSGEPLFLDSQAAASKDTAIHDVSFSHFYADIADDSETVAMIKELLETRIRPTVQDDGGDIVYCGFDSDTGIVKLKMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELDAEEEEAALSGQTE*