AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133080 MtrunA17_Chr1g0202581 90.909 132 12 0 1 132 1 132 2.06e-67 199
Msa0133080 MtrunA17_Chr1g0204291 90.152 132 13 0 1 132 1 132 5.85e-66 196
Msa0133080 MtrunA17_Chr1g0202541 83.824 136 18 2 1 132 1 136 3.74e-59 179
Msa0133080 MtrunA17_Chr1g0202561 77.143 140 23 4 1 132 1 139 1.95e-49 154
Msa0133080 MtrunA17_Chr6g0469571 59.259 135 39 5 1 131 1 123 1.85e-35 118
Msa0133080 MtrunA17_Chr6g0469551 58.519 135 40 5 1 131 1 123 5.45e-35 117
Msa0133080 MtrunA17_Chr6g0469591 57.778 135 41 5 1 131 1 123 1.59e-33 114
Msa0133080 MtrunA17_Chr8g0387471 49.254 134 57 3 1 131 1 126 1.36e-32 111
Msa0133080 MtrunA17_Chr6g0469651 53.957 139 49 4 1 131 1 132 6.29e-31 107
Msa0133080 MtrunA17_Chr6g0469681 54.412 136 47 5 1 131 1 126 8.47e-31 107
Msa0133080 MtrunA17_Chr6g0469671 68.571 70 21 1 1 69 1 70 2.02e-29 102
Msa0133080 MtrunA17_Chr7g0233181 45.763 118 47 4 1 116 1 103 5.20e-28 99.4
Msa0133080 MtrunA17_Chr7g0233251 45.000 120 49 4 1 118 1 105 3.95e-27 97.1
Msa0133080 MtrunA17_Chr1g0202501 52.555 137 51 5 1 131 1 129 4.19e-27 97.8
Msa0133080 MtrunA17_Chr6g0469541 46.667 120 47 5 1 118 1 105 6.84e-27 96.7
Msa0133080 MtrunA17_Chr7g0233191 45.833 120 48 5 1 118 1 105 9.79e-27 96.3
Msa0133080 MtrunA17_Chr7g0233201 44.167 120 50 5 1 118 1 105 6.44e-26 94.0
Msa0133080 MtrunA17_Chr7g0233231 58.667 75 30 1 1 74 1 75 4.23e-25 92.0
Msa0133080 MtrunA17_Chr7g0233211 44.167 120 50 5 1 118 1 105 4.47e-25 92.0
Msa0133080 MtrunA17_Chr7g0248671 57.353 68 28 1 1 67 1 68 2.41e-19 79.0
Msa0133080 MtrunA17_Chr3g0120541 43.210 81 40 4 2 76 4 84 1.70e-12 59.7
Msa0133080 MtrunA17_Chr8g0348361 41.026 156 59 3 1 131 1 148 4.90e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133080 AT5G52740.1 44.615 130 58 4 1 130 1 116 1.27e-22 86.3
Msa0133080 AT5G52760.1 40.152 132 69 5 2 132 4 126 6.23e-20 79.7
Msa0133080 AT1G01490.1 56.338 71 29 2 1 69 1 71 9.95e-18 75.1
Msa0133080 AT1G01490.2 56.338 71 29 2 1 69 1 71 9.95e-18 75.1
Msa0133080 AT1G01490.4 55.072 69 29 2 3 69 30 98 3.26e-16 72.0
Msa0133080 AT5G52750.1 49.231 65 32 1 3 66 5 69 1.13e-12 61.2
Msa0133080 AT5G52740.2 41.509 106 48 4 25 130 11 102 1.15e-12 60.5

Find 44 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAAATATCCTCATATTTA+AGG 0.068044 1_3:+64353752 Msa0133080:three_prime_UTR
AAGGCAGTCTCTGGCCTTTC+AGG 0.156873 1_3:+64352895 Msa0133080:CDS
CTTAGAGGTTGTATATATTA+TGG 0.223211 1_3:-64353779 None:intergenic
AGGATCCTCTTCAACGCTTA+TGG 0.283035 1_3:-64353409 None:intergenic
AAAGAAAGTTGAACCTAAAA+AGG 0.301808 1_3:+64353269 Msa0133080:CDS
TTGATGCAAATTGTAATAGA+AGG 0.314402 1_3:-64352576 None:intergenic
GGAAAGATTGAAGTGGTAAT+AGG 0.347890 1_3:-64352554 None:intergenic
TGGAATCATCTGATGATAAT+AGG 0.348261 1_3:-64353373 None:intergenic
ATTAATCCTTTCATGTACTA+TGG 0.359933 1_3:+64353336 Msa0133080:CDS
AACTAACCTTCATATTGTTA+TGG 0.378474 1_3:-64352627 None:intergenic
TGTATTAAATTGTGGATTGA+TGG 0.388592 1_3:-64353678 None:intergenic
ACAGAAAATTGTGCTAAAGT+TGG 0.399453 1_3:+64352837 Msa0133080:intron
ATGAAAGGATTAATTATCAT+TGG 0.400695 1_3:-64353327 None:intergenic
GCGAGACTATGTAAGGGCTT+TGG 0.416337 1_3:+64353653 Msa0133080:three_prime_UTR
TATATGATGACACAGCCATT+AGG 0.441354 1_3:-64353429 None:intergenic
AGCTTAGCCACTACACGTAC+TGG 0.441404 1_3:-64353195 None:intergenic
TGGGTCGGTATCTCCTACTA+AGG 0.446407 1_3:-64353175 None:intergenic
GACAAGAAAATGACCTTAGT+AGG 0.452020 1_3:+64353162 Msa0133080:CDS
GAGGCTTGCGAGACTATGTA+AGG 0.472462 1_3:+64353646 Msa0133080:three_prime_UTR
AAAGCTATGAAGGCAGTCTC+TGG 0.474866 1_3:+64352886 Msa0133080:CDS
TTCAGTTGGACCAGCTAAAG+AGG 0.475094 1_3:+64353245 Msa0133080:CDS
ATTGAAGTGGTAATAGGAAG+AGG 0.492767 1_3:-64352548 None:intergenic
TCTACTAATGTATTAAATTG+TGG 0.499922 1_3:-64353686 None:intergenic
CATGCTGAAATACTTTCAGT+TGG 0.503143 1_3:+64353231 Msa0133080:CDS
CTTATGGCACGGCAGTATTG+TGG 0.530914 1_3:-64353393 None:intergenic
ATGAGGATATTTGCGCTAAG+AGG 0.538142 1_3:-64353744 None:intergenic
TGATGATAATAGGTAGTGGC+AGG 0.538858 1_3:-64353363 None:intergenic
CCGTGCCATAAGCGTTGAAG+AGG 0.554104 1_3:+64353404 Msa0133080:CDS
TAGCTTCCATAACAATATGA+AGG 0.555459 1_3:+64352621 Msa0133080:exon
TGATGCAAATTGTAATAGAA+GGG 0.565633 1_3:-64352575 None:intergenic
TACGTGTAGTGGCTAAGCTA+AGG 0.568064 1_3:+64353199 Msa0133080:CDS
GCTTAGCCACTACACGTACT+GGG 0.574746 1_3:-64353194 None:intergenic
AGAGTTCTTCTACCTTAGCT+CGG 0.576900 1_3:+64352598 Msa0133080:five_prime_UTR
CATCTGATGATAATAGGTAG+TGG 0.579296 1_3:-64353367 None:intergenic
TTATGGAAGCTACCGAGCTA+AGG 0.591123 1_3:-64352610 None:intergenic
AGCGTTGAAGAGGATCCTAA+TGG 0.594188 1_3:+64353414 Msa0133080:CDS
TGATAATAGGTAGTGGCAGG+TGG 0.606289 1_3:-64353360 None:intergenic
GGTGGACCATAGTACATGAA+AGG 0.612109 1_3:-64353342 None:intergenic
TACCGACCCAGTACGTGTAG+TGG 0.629525 1_3:+64353188 Msa0133080:CDS
AGCCACTACACGTACTGGGT+CGG 0.643878 1_3:-64353190 None:intergenic
ATAGAAGGGAAAGATTGAAG+TGG 0.651637 1_3:-64352561 None:intergenic
CCTCTTCAACGCTTATGGCA+CGG 0.656572 1_3:-64353404 None:intergenic
AGTGTGTCGATCATAGTATG+AGG 0.659198 1_3:+64353627 Msa0133080:three_prime_UTR
AGGCTTGCGAGACTATGTAA+GGG 0.668349 1_3:+64353647 Msa0133080:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTATTTTAATAATTTTGC+AGG + chr1_3:64353114-64353133 Msa0133080:intron 15.0%
!!! TGTATTTTAATAATTTTGCA+GGG + chr1_3:64353115-64353134 Msa0133080:intron 15.0%
!!! AGAAAAATAATCATTTTTTG+AGG + chr1_3:64353491-64353510 Msa0133080:three_prime_UTR 15.0%
!!! TTGTATTTTAATAATTTTGC+AGG + chr1_3:64353114-64353133 Msa0133080:intron 15.0%
!!! TGTATTTTAATAATTTTGCA+GGG + chr1_3:64353115-64353134 Msa0133080:intron 15.0%
!!! AGAAAAATAATCATTTTTTG+AGG + chr1_3:64353491-64353510 Msa0133080:three_prime_UTR 15.0%
!!! AAAAAATCTACTTGTTAGAA+CGG - chr1_3:64352812-64352831 None:intergenic 20.0%
!!! ATTTTTTTTCTTTACCTGAA+AGG - chr1_3:64352912-64352931 None:intergenic 20.0%
!!! GTATTTTAATAATTTTGCAG+GGG + chr1_3:64353116-64353135 Msa0133080:intron 20.0%
!!! TTCTTTTTATCATCCTTTTT+AGG - chr1_3:64353285-64353304 None:intergenic 20.0%
!! TAAAAAGGATGATAAAAAGA+AGG + chr1_3:64353284-64353303 Msa0133080:CDS 20.0%
!! ATGAAAGGATTAATTATCAT+TGG - chr1_3:64353330-64353349 None:intergenic 20.0%
!!! ATTATATACTTTTTTGAGAG+AGG + chr1_3:64353533-64353552 Msa0133080:three_prime_UTR 20.0%
!!! TTATATACTTTTTTGAGAGA+GGG + chr1_3:64353534-64353553 Msa0133080:three_prime_UTR 20.0%
!! TCTACTAATGTATTAAATTG+TGG - chr1_3:64353689-64353708 None:intergenic 20.0%
!!! AAAAAATCTACTTGTTAGAA+CGG - chr1_3:64352812-64352831 None:intergenic 20.0%
!!! ATTTTTTTTCTTTACCTGAA+AGG - chr1_3:64352912-64352931 None:intergenic 20.0%
!!! GTATTTTAATAATTTTGCAG+GGG + chr1_3:64353116-64353135 Msa0133080:intron 20.0%
!!! TTCTTTTTATCATCCTTTTT+AGG - chr1_3:64353285-64353304 None:intergenic 20.0%
!! TAAAAAGGATGATAAAAAGA+AGG + chr1_3:64353284-64353303 Msa0133080:CDS 20.0%
!! ATGAAAGGATTAATTATCAT+TGG - chr1_3:64353330-64353349 None:intergenic 20.0%
!!! ATTATATACTTTTTTGAGAG+AGG + chr1_3:64353533-64353552 Msa0133080:three_prime_UTR 20.0%
!!! TTATATACTTTTTTGAGAGA+GGG + chr1_3:64353534-64353553 Msa0133080:three_prime_UTR 20.0%
!! TCTACTAATGTATTAAATTG+TGG - chr1_3:64353689-64353708 None:intergenic 20.0%
! TGATGCAAATTGTAATAGAA+GGG - chr1_3:64352578-64352597 None:intergenic 25.0%
! TTGATGCAAATTGTAATAGA+AGG - chr1_3:64352579-64352598 None:intergenic 25.0%
! AACTAACCTTCATATTGTTA+TGG - chr1_3:64352630-64352649 None:intergenic 25.0%
!!! TAAAAGAGTGGTTTTAATCA+AGG - chr1_3:64352662-64352681 None:intergenic 25.0%
! TCATACTTTAGCTATGATAT+AGG - chr1_3:64352688-64352707 None:intergenic 25.0%
! TATCATAGCTAAAGTATGAT+TGG + chr1_3:64352689-64352708 Msa0133080:intron 25.0%
! TTGAAATTGTGCATAAGAAA+AGG - chr1_3:64352723-64352742 None:intergenic 25.0%
! AATCAAACAAAAAGCTATGA+AGG + chr1_3:64352876-64352895 Msa0133080:CDS 25.0%
! ATCGAACTTAGTCTAATATA+TGG - chr1_3:64353003-64353022 None:intergenic 25.0%
!!! ACTAAGTTCGATATTTTGAT+TGG + chr1_3:64353011-64353030 Msa0133080:intron 25.0%
!!! AATCATTGATTCTACGAATT+TGG - chr1_3:64353059-64353078 None:intergenic 25.0%
! AAAGAAAGTTGAACCTAAAA+AGG + chr1_3:64353269-64353288 Msa0133080:CDS 25.0%
! ATTAATCCTTTCATGTACTA+TGG + chr1_3:64353336-64353355 Msa0133080:CDS 25.0%
!! TGTATTAAATTGTGGATTGA+TGG - chr1_3:64353681-64353700 None:intergenic 25.0%
!! TATGGTTTTCCTTAAATATG+AGG - chr1_3:64353764-64353783 None:intergenic 25.0%
! CTTAGAGGTTGTATATATTA+TGG - chr1_3:64353782-64353801 None:intergenic 25.0%
! TGATGCAAATTGTAATAGAA+GGG - chr1_3:64352578-64352597 None:intergenic 25.0%
! TTGATGCAAATTGTAATAGA+AGG - chr1_3:64352579-64352598 None:intergenic 25.0%
! AACTAACCTTCATATTGTTA+TGG - chr1_3:64352630-64352649 None:intergenic 25.0%
!!! TAAAAGAGTGGTTTTAATCA+AGG - chr1_3:64352662-64352681 None:intergenic 25.0%
! TCATACTTTAGCTATGATAT+AGG - chr1_3:64352688-64352707 None:intergenic 25.0%
! TATCATAGCTAAAGTATGAT+TGG + chr1_3:64352689-64352708 Msa0133080:intron 25.0%
! TTGAAATTGTGCATAAGAAA+AGG - chr1_3:64352723-64352742 None:intergenic 25.0%
! AATCAAACAAAAAGCTATGA+AGG + chr1_3:64352876-64352895 Msa0133080:CDS 25.0%
! ATCGAACTTAGTCTAATATA+TGG - chr1_3:64353003-64353022 None:intergenic 25.0%
!!! ACTAAGTTCGATATTTTGAT+TGG + chr1_3:64353011-64353030 Msa0133080:intron 25.0%
!!! AATCATTGATTCTACGAATT+TGG - chr1_3:64353059-64353078 None:intergenic 25.0%
! AAAGAAAGTTGAACCTAAAA+AGG + chr1_3:64353269-64353288 Msa0133080:CDS 25.0%
! ATTAATCCTTTCATGTACTA+TGG + chr1_3:64353336-64353355 Msa0133080:CDS 25.0%
!! TGTATTAAATTGTGGATTGA+TGG - chr1_3:64353681-64353700 None:intergenic 25.0%
!! TATGGTTTTCCTTAAATATG+AGG - chr1_3:64353764-64353783 None:intergenic 25.0%
! CTTAGAGGTTGTATATATTA+TGG - chr1_3:64353782-64353801 None:intergenic 25.0%
TAGCTTCCATAACAATATGA+AGG + chr1_3:64352621-64352640 Msa0133080:exon 30.0%
TGATATAGGAGATAAAAGAG+TGG - chr1_3:64352674-64352693 None:intergenic 30.0%
ACAGAAAATTGTGCTAAAGT+TGG + chr1_3:64352837-64352856 Msa0133080:intron 30.0%
TGGAATCATCTGATGATAAT+AGG - chr1_3:64353376-64353395 None:intergenic 30.0%
CGCAAATATCCTCATATTTA+AGG + chr1_3:64353752-64353771 Msa0133080:three_prime_UTR 30.0%
TAGCTTCCATAACAATATGA+AGG + chr1_3:64352621-64352640 Msa0133080:exon 30.0%
TGATATAGGAGATAAAAGAG+TGG - chr1_3:64352674-64352693 None:intergenic 30.0%
ACAGAAAATTGTGCTAAAGT+TGG + chr1_3:64352837-64352856 Msa0133080:intron 30.0%
TGGAATCATCTGATGATAAT+AGG - chr1_3:64353376-64353395 None:intergenic 30.0%
CGCAAATATCCTCATATTTA+AGG + chr1_3:64353752-64353771 Msa0133080:three_prime_UTR 30.0%
ATTGAAGTGGTAATAGGAAG+AGG - chr1_3:64352551-64352570 None:intergenic 35.0%
GGAAAGATTGAAGTGGTAAT+AGG - chr1_3:64352557-64352576 None:intergenic 35.0%
ATAGAAGGGAAAGATTGAAG+TGG - chr1_3:64352564-64352583 None:intergenic 35.0%
! TACCAAAGTGCTTCAAATTC+AGG - chr1_3:64352747-64352766 None:intergenic 35.0%
TTGATTGGACAATTCAGATG+TGG + chr1_3:64353026-64353045 Msa0133080:intron 35.0%
GACAAGAAAATGACCTTAGT+AGG + chr1_3:64353162-64353181 Msa0133080:CDS 35.0%
CATGCTGAAATACTTTCAGT+TGG + chr1_3:64353231-64353250 Msa0133080:CDS 35.0%
! ACTTTCTTTTCCTCTTTAGC+TGG - chr1_3:64353258-64353277 None:intergenic 35.0%
CATCTGATGATAATAGGTAG+TGG - chr1_3:64353370-64353389 None:intergenic 35.0%
TATATGATGACACAGCCATT+AGG - chr1_3:64353432-64353451 None:intergenic 35.0%
ATTGAAGTGGTAATAGGAAG+AGG - chr1_3:64352551-64352570 None:intergenic 35.0%
GGAAAGATTGAAGTGGTAAT+AGG - chr1_3:64352557-64352576 None:intergenic 35.0%
ATAGAAGGGAAAGATTGAAG+TGG - chr1_3:64352564-64352583 None:intergenic 35.0%
! TACCAAAGTGCTTCAAATTC+AGG - chr1_3:64352747-64352766 None:intergenic 35.0%
TTGATTGGACAATTCAGATG+TGG + chr1_3:64353026-64353045 Msa0133080:intron 35.0%
GACAAGAAAATGACCTTAGT+AGG + chr1_3:64353162-64353181 Msa0133080:CDS 35.0%
CATGCTGAAATACTTTCAGT+TGG + chr1_3:64353231-64353250 Msa0133080:CDS 35.0%
! ACTTTCTTTTCCTCTTTAGC+TGG - chr1_3:64353258-64353277 None:intergenic 35.0%
CATCTGATGATAATAGGTAG+TGG - chr1_3:64353370-64353389 None:intergenic 35.0%
TATATGATGACACAGCCATT+AGG - chr1_3:64353432-64353451 None:intergenic 35.0%
AGAGTTCTTCTACCTTAGCT+CGG + chr1_3:64352598-64352617 Msa0133080:five_prime_UTR 40.0%
! AGCCTGAATTTGAAGCACTT+TGG + chr1_3:64352742-64352761 Msa0133080:intron 40.0%
! TGATGATAATAGGTAGTGGC+AGG - chr1_3:64353366-64353385 None:intergenic 40.0%
! AGTGTGTCGATCATAGTATG+AGG + chr1_3:64353627-64353646 Msa0133080:three_prime_UTR 40.0%
ATGAGGATATTTGCGCTAAG+AGG - chr1_3:64353747-64353766 None:intergenic 40.0%
AGAGTTCTTCTACCTTAGCT+CGG + chr1_3:64352598-64352617 Msa0133080:five_prime_UTR 40.0%
! AGCCTGAATTTGAAGCACTT+TGG + chr1_3:64352742-64352761 Msa0133080:intron 40.0%
! TGATGATAATAGGTAGTGGC+AGG - chr1_3:64353366-64353385 None:intergenic 40.0%
! AGTGTGTCGATCATAGTATG+AGG + chr1_3:64353627-64353646 Msa0133080:three_prime_UTR 40.0%
ATGAGGATATTTGCGCTAAG+AGG - chr1_3:64353747-64353766 None:intergenic 40.0%
TTATGGAAGCTACCGAGCTA+AGG - chr1_3:64352613-64352632 None:intergenic 45.0%
AAAGCTATGAAGGCAGTCTC+TGG + chr1_3:64352886-64352905 Msa0133080:CDS 45.0%
TACGTGTAGTGGCTAAGCTA+AGG + chr1_3:64353199-64353218 Msa0133080:CDS 45.0%
TTCAGTTGGACCAGCTAAAG+AGG + chr1_3:64353245-64353264 Msa0133080:CDS 45.0%
GGTGGACCATAGTACATGAA+AGG - chr1_3:64353345-64353364 None:intergenic 45.0%
! TGATAATAGGTAGTGGCAGG+TGG - chr1_3:64353363-64353382 None:intergenic 45.0%
AGGATCCTCTTCAACGCTTA+TGG - chr1_3:64353412-64353431 None:intergenic 45.0%
! AGCGTTGAAGAGGATCCTAA+TGG + chr1_3:64353414-64353433 Msa0133080:CDS 45.0%
AGGCTTGCGAGACTATGTAA+GGG + chr1_3:64353647-64353666 Msa0133080:three_prime_UTR 45.0%
TTATGGAAGCTACCGAGCTA+AGG - chr1_3:64352613-64352632 None:intergenic 45.0%
AAAGCTATGAAGGCAGTCTC+TGG + chr1_3:64352886-64352905 Msa0133080:CDS 45.0%
TACGTGTAGTGGCTAAGCTA+AGG + chr1_3:64353199-64353218 Msa0133080:CDS 45.0%
TTCAGTTGGACCAGCTAAAG+AGG + chr1_3:64353245-64353264 Msa0133080:CDS 45.0%
GGTGGACCATAGTACATGAA+AGG - chr1_3:64353345-64353364 None:intergenic 45.0%
! TGATAATAGGTAGTGGCAGG+TGG - chr1_3:64353363-64353382 None:intergenic 45.0%
AGGATCCTCTTCAACGCTTA+TGG - chr1_3:64353412-64353431 None:intergenic 45.0%
! AGCGTTGAAGAGGATCCTAA+TGG + chr1_3:64353414-64353433 Msa0133080:CDS 45.0%
AGGCTTGCGAGACTATGTAA+GGG + chr1_3:64353647-64353666 Msa0133080:three_prime_UTR 45.0%
TGGGTCGGTATCTCCTACTA+AGG - chr1_3:64353178-64353197 None:intergenic 50.0%
GCTTAGCCACTACACGTACT+GGG - chr1_3:64353197-64353216 None:intergenic 50.0%
AGCTTAGCCACTACACGTAC+TGG - chr1_3:64353198-64353217 None:intergenic 50.0%
CTTATGGCACGGCAGTATTG+TGG - chr1_3:64353396-64353415 None:intergenic 50.0%
CCTCTTCAACGCTTATGGCA+CGG - chr1_3:64353407-64353426 None:intergenic 50.0%
GAGGCTTGCGAGACTATGTA+AGG + chr1_3:64353646-64353665 Msa0133080:three_prime_UTR 50.0%
GCGAGACTATGTAAGGGCTT+TGG + chr1_3:64353653-64353672 Msa0133080:three_prime_UTR 50.0%
TGGGTCGGTATCTCCTACTA+AGG - chr1_3:64353178-64353197 None:intergenic 50.0%
GCTTAGCCACTACACGTACT+GGG - chr1_3:64353197-64353216 None:intergenic 50.0%
AGCTTAGCCACTACACGTAC+TGG - chr1_3:64353198-64353217 None:intergenic 50.0%
CTTATGGCACGGCAGTATTG+TGG - chr1_3:64353396-64353415 None:intergenic 50.0%
CCTCTTCAACGCTTATGGCA+CGG - chr1_3:64353407-64353426 None:intergenic 50.0%
GAGGCTTGCGAGACTATGTA+AGG + chr1_3:64353646-64353665 Msa0133080:three_prime_UTR 50.0%
GCGAGACTATGTAAGGGCTT+TGG + chr1_3:64353653-64353672 Msa0133080:three_prime_UTR 50.0%
! AAGGCAGTCTCTGGCCTTTC+AGG + chr1_3:64352895-64352914 Msa0133080:CDS 55.0%
TACCGACCCAGTACGTGTAG+TGG + chr1_3:64353188-64353207 Msa0133080:CDS 55.0%
AGCCACTACACGTACTGGGT+CGG - chr1_3:64353193-64353212 None:intergenic 55.0%
!! CCGTGCCATAAGCGTTGAAG+AGG + chr1_3:64353404-64353423 Msa0133080:CDS 55.0%
! AAGGCAGTCTCTGGCCTTTC+AGG + chr1_3:64352895-64352914 Msa0133080:CDS 55.0%
TACCGACCCAGTACGTGTAG+TGG + chr1_3:64353188-64353207 Msa0133080:CDS 55.0%
AGCCACTACACGTACTGGGT+CGG - chr1_3:64353193-64353212 None:intergenic 55.0%
!! CCGTGCCATAAGCGTTGAAG+AGG + chr1_3:64353404-64353423 Msa0133080:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64352516 64353804 64352516 ID=Msa0133080;Name=Msa0133080
chr1_3 mRNA 64352516 64353804 64352516 ID=Msa0133080-mRNA-1;Parent=Msa0133080;Name=Msa0133080-mRNA-1;_AED=0.27;_eAED=0.27;_QI=121|1|1|1|1|1|3|352|132
chr1_3 exon 64352516 64352642 64352516 ID=Msa0133080-mRNA-1:exon:21637;Parent=Msa0133080-mRNA-1
chr1_3 exon 64352841 64352916 64352841 ID=Msa0133080-mRNA-1:exon:21638;Parent=Msa0133080-mRNA-1
chr1_3 exon 64353136 64353804 64353136 ID=Msa0133080-mRNA-1:exon:21639;Parent=Msa0133080-mRNA-1
chr1_3 five_prime_UTR 64352516 64352636 64352516 ID=Msa0133080-mRNA-1:five_prime_utr;Parent=Msa0133080-mRNA-1
chr1_3 CDS 64352637 64352642 64352637 ID=Msa0133080-mRNA-1:cds;Parent=Msa0133080-mRNA-1
chr1_3 CDS 64352841 64352916 64352841 ID=Msa0133080-mRNA-1:cds;Parent=Msa0133080-mRNA-1
chr1_3 CDS 64353136 64353452 64353136 ID=Msa0133080-mRNA-1:cds;Parent=Msa0133080-mRNA-1
chr1_3 three_prime_UTR 64353453 64353804 64353453 ID=Msa0133080-mRNA-1:three_prime_utr;Parent=Msa0133080-mRNA-1
Gene Sequence

>Msa0133080

ATGAAGAAAATTGTGCTAAAGTTGGAGATTCATGAAGACAAAATCAAACAAAAAGCTATGAAGGCAGTCTCTGGCCTTTCAGGGGTTGAGTCAGTTGAAGTAGACAAAGACAAGAAAATGACCTTAGTAGGAGATACCGACCCAGTACGTGTAGTGGCTAAGCTAAGGAAGATTTGTCATGCTGAAATACTTTCAGTTGGACCAGCTAAAGAGGAAAAGAAAGTTGAACCTAAAAAGGATGATAAAAAGAAGGAAGATGATAAGAAAGATTCACCAATGATAATTAATCCTTTCATGTACTATGGTCCACCTGCCACTACCTATTATCATCAGATGATTCCACAATACTGCCGTGCCATAAGCGTTGAAGAGGATCCTAATGGCTGTGTCATCATATAA
Protein sequence

>Msa0133080

MKKIVLKLEIHEDKIKQKAMKAVSGLSGVESVEVDKDKKMTLVGDTDPVRVVAKLRKICHAEILSVGPAKEEKKVEPKKDDKKKEDDKKDSPMIINPFMYYGPPATTYYHQMIPQYCRAISVEEDPNGCVII*