AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133760 MtrunA17_Chr1g0203401 92.958 213 10 2 1 212 1 209 1.67e-117 332
Msa0133760 MtrunA17_Chr8g0349271 60.335 179 62 2 34 212 31 200 5.26e-66 202
Msa0133760 MtrunA17_Chr8g0386521 58.400 125 52 0 88 212 74 198 1.53e-47 155
Msa0133760 MtrunA17_Chr5g0397781 61.475 122 47 0 91 212 86 207 1.52e-46 152
Msa0133760 MtrunA17_Chr7g0247121 69.231 65 20 0 148 212 34 98 4.90e-26 96.7
Msa0133760 MtrunA17_Chr1g0173571 33.607 122 81 0 86 207 148 269 1.67e-14 70.5
Msa0133760 MtrunA17_Chr1g0153281 35.577 104 66 1 109 212 513 615 2.24e-12 65.5
Msa0133760 MtrunA17_Chr7g0257771 34.959 123 79 1 85 207 154 275 4.81e-12 63.5
Msa0133760 MtrunA17_Chr8g0376741 38.532 109 64 1 97 205 412 517 5.13e-12 64.3
Msa0133760 MtrunA17_Chr8g0377061 34.615 104 68 0 109 212 251 354 5.70e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133760 AT5G23750.2 70.667 150 38 2 64 212 57 201 8.45e-66 202
Msa0133760 AT3G61260.1 62.791 172 57 3 42 212 47 212 2.33e-65 201
Msa0133760 AT3G48940.1 61.628 172 59 3 46 212 6 175 8.39e-65 198
Msa0133760 AT5G23750.1 77.778 126 28 0 87 212 77 202 1.28e-64 199
Msa0133760 AT5G23750.3 77.778 126 28 0 87 212 104 229 1.66e-64 199
Msa0133760 AT2G45820.1 61.176 170 57 2 43 212 30 190 1.81e-54 172
Msa0133760 AT1G63295.1 44.628 121 64 1 95 212 31 151 5.10e-22 88.2
Msa0133760 AT1G63295.2 46.364 110 59 0 95 204 71 180 9.17e-22 88.6
Msa0133760 AT4G00670.1 42.157 102 59 0 106 207 16 117 2.73e-20 82.8
Msa0133760 AT2G41870.1 33.884 121 79 1 93 212 153 273 5.23e-13 66.6
Msa0133760 AT3G57540.1 33.058 121 79 1 93 211 175 295 1.58e-12 65.5
Msa0133760 AT1G67590.1 29.412 187 126 2 30 212 154 338 3.85e-12 64.7
Msa0133760 AT1G30320.1 39.167 120 66 2 97 212 392 508 4.24e-12 65.1
Msa0133760 AT2G02170.1 42.308 104 60 0 109 212 379 482 6.94e-11 61.2
Msa0133760 AT2G02170.2 42.308 104 60 0 109 212 379 482 6.94e-11 61.2
Msa0133760 AT2G02170.5 42.308 104 60 0 109 212 379 482 6.94e-11 61.2
Msa0133760 AT2G02170.4 42.308 104 60 0 109 212 379 482 6.94e-11 61.2
Msa0133760 AT2G02170.3 42.308 104 60 0 109 212 254 357 6.96e-11 61.2
Msa0133760 AT2G02170.6 42.308 104 60 0 109 212 254 357 6.96e-11 61.2

Find 68 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGACCAAAGAGTTTCTT+TGG 0.217523 1_3:-65096507 None:intergenic
ATTACAAGTGTTGGAGAGTT+AGG 0.248611 1_3:-65094266 None:intergenic
TCTTAGCTGGGTGGGTTTAT+TGG 0.252899 1_3:-65094161 None:intergenic
TCCTCAGCTACATCTTTCTT+TGG 0.259226 1_3:-65094523 None:intergenic
TCGATAGAGCCGCCCTCTTT+TGG 0.297765 1_3:-65095826 None:intergenic
GGAACAACTAGAGAAGAAAA+AGG 0.300679 1_3:+65096338 Msa0133760:intron
TCTTTCTAAGAAAGAAGAAT+TGG 0.340874 1_3:-65094368 None:intergenic
GTTGTATATATCCATTACTT+TGG 0.347191 1_3:+65096667 Msa0133760:three_prime_UTR
TTGTATATATCCATTACTTT+GGG 0.362237 1_3:+65096668 Msa0133760:three_prime_UTR
ACATCTTTCTTTGGAGCCTC+AGG 0.378728 1_3:-65094514 None:intergenic
ACTGTTCTCCCATGCTGTTA+TGG 0.379485 1_3:-65096189 None:intergenic
ATTCTACTAAAAGTCTTAGC+TGG 0.382428 1_3:-65094174 None:intergenic
GCCTCAGGGAGAGGAGGAGA+AGG 0.390077 1_3:-65094499 None:intergenic
ACAGCTCCAAAGAAACTCTT+TGG 0.398417 1_3:+65096501 Msa0133760:CDS
AGTTGGCACAGCGCACCAAT+AGG 0.402374 1_3:-65094124 None:intergenic
AAGAAAGATATTACAAGTGT+TGG 0.420015 1_3:-65094275 None:intergenic
AAGAGGTAGCTGCAAAATAC+AGG 0.422702 1_3:+65096469 Msa0133760:CDS
CTGAGCTATGACAAGAGCTT+TGG 0.429684 1_3:-65094602 None:intergenic
GCTGCAAAATACAGGGCAAC+TGG 0.430234 1_3:+65096477 Msa0133760:CDS
TTCTTTGGAGCCTCAGGGAG+AGG 0.442969 1_3:-65094508 None:intergenic
TTACAAGTGTTGGAGAGTTA+GGG 0.454246 1_3:-65094265 None:intergenic
AATAAGTTGTCTGTCCCTAT+TGG 0.457363 1_3:+65094109 None:intergenic
AGCTGGGTGGGTTTATTGGT+AGG 0.476393 1_3:-65094157 None:intergenic
TCAGTTGAAGCAGGTGGTGG+TGG 0.488620 1_3:-65094460 None:intergenic
GCTTTGGAGTCATCAACAAC+AGG 0.488845 1_3:-65094586 None:intergenic
GTTGAAGCAGGTGGTGGTGG+AGG 0.497469 1_3:-65094457 None:intergenic
ACTCACTCATTCTTAGATGT+TGG 0.501732 1_3:+65096754 Msa0133760:three_prime_UTR
GTTGGCACAGCGCACCAATA+GGG 0.504102 1_3:-65094123 None:intergenic
TATTATAGTTGTAGAATGAA+AGG 0.511225 1_3:-65094232 None:intergenic
GAGTCAGTGGTCTAATACAA+AGG 0.513455 1_3:-65096735 None:intergenic
ATTATAGTTGTAGAATGAAA+GGG 0.515536 1_3:-65094231 None:intergenic
TGCTACAATGTTCAAGATTG+TGG 0.518850 1_3:+65096534 Msa0133760:three_prime_UTR
CAAGGCAGCTGAAGAAAAGA+AGG 0.521517 1_3:+65096404 Msa0133760:CDS
TCATCAACAACAGGAGGAGG+AGG 0.524276 1_3:-65094577 None:intergenic
TTCTACTAAAAGTCTTAGCT+GGG 0.524449 1_3:-65094173 None:intergenic
TCAACAACAGGAGGAGGAGG+AGG 0.529147 1_3:-65094574 None:intergenic
CCTCAGGGAGAGGAGGAGAA+GGG 0.530917 1_3:-65094498 None:intergenic
TGGTAGGTACTCTTGTGAGT+TGG 0.531008 1_3:-65094141 None:intergenic
GGCTGTCACTAATCAAAGCA+TGG 0.531510 1_3:+65095975 Msa0133760:CDS
CTCAGGGAGAGGAGGAGAAG+GGG 0.532056 1_3:-65094497 None:intergenic
GTCTCAGTTGAAGCAGGTGG+TGG 0.537604 1_3:-65094463 None:intergenic
GGAGGAGGATTATTATCTTG+TGG 0.541469 1_3:-65094559 None:intergenic
CAAGAGTTGCAACAGAAAAG+AGG 0.549164 1_3:+65095954 Msa0133760:CDS
ACTAAAAGTCTTAGCTGGGT+GGG 0.558329 1_3:-65094169 None:intergenic
TTTGGAGCCTCAGGGAGAGG+AGG 0.558769 1_3:-65094505 None:intergenic
CATCTTTCTTTGGAGCCTCA+GGG 0.563937 1_3:-65094513 None:intergenic
TGCTGAAGAGAAACCAAAAG+AGG 0.570519 1_3:+65095813 Msa0133760:CDS
GAGTCATCAACAACAGGAGG+AGG 0.575285 1_3:-65094580 None:intergenic
TCCAAAGAAAGATGTAGCTG+AGG 0.578942 1_3:+65094522 Msa0133760:CDS
GCTATGATTGAGGCAAAGAA+AGG 0.579579 1_3:+65096426 Msa0133760:CDS
AACTTTCATCCATAACAGCA+TGG 0.579782 1_3:+65096180 Msa0133760:CDS
TACTAAAAGTCTTAGCTGGG+TGG 0.586125 1_3:-65094170 None:intergenic
CAAAATAGCAGCACTTCACA+AGG 0.592299 1_3:+65096386 Msa0133760:CDS
AAGTGTTGGAGAGTTAGGGT+TGG 0.592661 1_3:-65094261 None:intergenic
AGAAAAGAAGGCTATGATTG+AGG 0.593916 1_3:+65096416 Msa0133760:CDS
GTAGTCGTCTCAGTTGAAGC+AGG 0.595047 1_3:-65094469 None:intergenic
AGCTGAATTGAGAAAACTTG+AGG 0.599321 1_3:+65096229 Msa0133760:CDS
AGAATTACTCAAAGCTGAAG+AGG 0.604887 1_3:+65096452 Msa0133760:CDS
GCTGTCACTAATCAAAGCAT+GGG 0.606143 1_3:+65095976 Msa0133760:CDS
ACTTTCATCCATAACAGCAT+GGG 0.613867 1_3:+65096181 Msa0133760:CDS
CCCTTCTCCTCCTCTCCCTG+AGG 0.617803 1_3:+65094498 Msa0133760:CDS
AGAGGTAGCTGCAAAATACA+GGG 0.632532 1_3:+65096470 Msa0133760:CDS
GTCGTCTCAGTTGAAGCAGG+TGG 0.640903 1_3:-65094466 None:intergenic
TCTAAGAATGAGTGAGTCAG+TGG 0.643025 1_3:-65096748 None:intergenic
GCTGAAGAGAAACCAAAAGA+GGG 0.649888 1_3:+65095814 Msa0133760:CDS
TTGGAGTCATCAACAACAGG+AGG 0.656015 1_3:-65094583 None:intergenic
GAAGAGAAACCAAAAGAGGG+CGG 0.689508 1_3:+65095817 Msa0133760:CDS
GAGGGCGGCTCTATCGACCG+TGG 0.735676 1_3:+65095832 Msa0133760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTGTTTAAATTTTATTTTAT+TGG + chr1_3:65094665-65094684 Msa0133760:intron 10.0%
!!! ACAATTTTATTATAGAAAAA+AGG + chr1_3:65094699-65094718 Msa0133760:intron 10.0%
!! TTTGAAAAAAATCAATTATA+GGG - chr1_3:65094838-65094857 None:intergenic 10.0%
!!! AAATTATTACTTTTAATAGA+AGG + chr1_3:65095080-65095099 Msa0133760:intron 10.0%
!!! AATTATTACTTTTAATAGAA+GGG + chr1_3:65095081-65095100 Msa0133760:intron 10.0%
!!! TATGTTTTATCTATCTATTT+TGG + chr1_3:65094739-65094758 Msa0133760:intron 15.0%
!!! CTTTGAAAAAAATCAATTAT+AGG - chr1_3:65094839-65094858 None:intergenic 15.0%
!!! ATCAATACATATTTTTTATC+TGG + chr1_3:65094890-65094909 Msa0133760:intron 15.0%
!!! TTTAAATTTGTGCATATTTT+TGG - chr1_3:65095539-65095558 None:intergenic 15.0%
!! TGACTTAAAAACAAATATTA+CGG - chr1_3:65095716-65095735 None:intergenic 15.0%
!! ATATAATTCTGTTGTTAATT+TGG + chr1_3:65095766-65095785 Msa0133760:intron 15.0%
!!! AATTACTTTTTACTATGAAT+AGG + chr1_3:65096317-65096336 Msa0133760:intron 15.0%
!! ATTATAGTTGTAGAATGAAA+GGG - chr1_3:65094234-65094253 None:intergenic 20.0%
!! TATTATAGTTGTAGAATGAA+AGG - chr1_3:65094235-65094254 None:intergenic 20.0%
!! CTATAATAAAATTGTTTCCT+TGG - chr1_3:65094694-65094713 None:intergenic 20.0%
!!! TCTATTTTGGTTTGTTTTAT+TGG + chr1_3:65094752-65094771 Msa0133760:intron 20.0%
!! TAATAGCAAAATTGAATTGA+AGG + chr1_3:65094990-65095009 Msa0133760:intron 20.0%
!! TTAATTGATTAGATTGTAGT+TGG - chr1_3:65095383-65095402 None:intergenic 20.0%
!! AATCTAATCAATTAAGTGTA+TGG + chr1_3:65095388-65095407 Msa0133760:intron 20.0%
!!! TAATGCTTAATGTGTATTTT+TGG + chr1_3:65096048-65096067 Msa0133760:intron 20.0%
!! TTGTATATATCCATTACTTT+GGG + chr1_3:65096668-65096687 Msa0133760:three_prime_UTR 20.0%
! AAGAAAGATATTACAAGTGT+TGG - chr1_3:65094278-65094297 None:intergenic 25.0%
!!! AAGAATTGGAACAACTTTTT+TGG - chr1_3:65094357-65094376 None:intergenic 25.0%
!! TCTTTCTAAGAAAGAAGAAT+TGG - chr1_3:65094371-65094390 None:intergenic 25.0%
!!! GATTCAGATTCTATCTTTTT+TGG - chr1_3:65094427-65094446 None:intergenic 25.0%
!!! TTTTATTTTATTGGTTGCCA+AGG + chr1_3:65094674-65094693 Msa0133760:intron 25.0%
! TTATAGGGAAAATTTGAAGA+TGG - chr1_3:65094823-65094842 None:intergenic 25.0%
! AAACATGCATTAAGATTCTA+GGG + chr1_3:65095488-65095507 Msa0133760:intron 25.0%
!!! AAAACGGTAAAGGTTATTTT+GGG - chr1_3:65095672-65095691 None:intergenic 25.0%
! AAACAAATATTACGGATGAA+TGG - chr1_3:65095708-65095727 None:intergenic 25.0%
! TATATCAATGTGATTATACG+CGG - chr1_3:65095751-65095770 None:intergenic 25.0%
! AATACACATTAAGCATTAGA+TGG - chr1_3:65096046-65096065 None:intergenic 25.0%
! TACTAATCTCCTTAGAATTA+TGG - chr1_3:65096111-65096130 None:intergenic 25.0%
! ACATTGAATGTGACAAAATA+TGG + chr1_3:65096278-65096297 Msa0133760:intron 25.0%
! CATTGAATGTGACAAAATAT+GGG + chr1_3:65096279-65096298 Msa0133760:intron 25.0%
! ATTGAATGTGACAAAATATG+GGG + chr1_3:65096280-65096299 Msa0133760:intron 25.0%
! GTTGTATATATCCATTACTT+TGG + chr1_3:65096667-65096686 Msa0133760:three_prime_UTR 25.0%
!!! TGTTTTTATTCCCAAAGTAA+TGG - chr1_3:65096681-65096700 None:intergenic 25.0%
TTCTACTAAAAGTCTTAGCT+GGG - chr1_3:65094176-65094195 None:intergenic 30.0%
ATTCTACTAAAAGTCTTAGC+TGG - chr1_3:65094177-65094196 None:intergenic 30.0%
!!! TTTGGTTTGTTTTATTGGAC+TGG + chr1_3:65094757-65094776 Msa0133760:intron 30.0%
!!! TGTTTTATTGGACTGGTATA+AGG + chr1_3:65094764-65094783 Msa0133760:intron 30.0%
ATATAAGCGAAATAGTTGAG+AGG + chr1_3:65094933-65094952 Msa0133760:intron 30.0%
GGAAACTGAGAAAAATACAA+GGG - chr1_3:65095283-65095302 None:intergenic 30.0%
TGGAAACTGAGAAAAATACA+AGG - chr1_3:65095284-65095303 None:intergenic 30.0%
ATCAGAAAAATTCCAGGAAA+TGG - chr1_3:65095304-65095323 None:intergenic 30.0%
ACAATCATCAGAAAAATTCC+AGG - chr1_3:65095310-65095329 None:intergenic 30.0%
TTGATTAGATTGTAGTTGGA+TGG - chr1_3:65095379-65095398 None:intergenic 30.0%
CTAGAATCTTAATGCATGTT+TGG - chr1_3:65095489-65095508 None:intergenic 30.0%
CAAACATGCATTAAGATTCT+AGG + chr1_3:65095487-65095506 Msa0133760:intron 30.0%
AGAAAAGAAACACATTAGCT+TGG + chr1_3:65095558-65095577 Msa0133760:intron 30.0%
! GAAAACGGTAAAGGTTATTT+TGG - chr1_3:65095673-65095692 None:intergenic 30.0%
CTAAAACATGAAAACGGTAA+AGG - chr1_3:65095682-65095701 None:intergenic 30.0%
! CTTTACCGTTTTCATGTTTT+AGG + chr1_3:65095680-65095699 Msa0133760:intron 30.0%
TGGTTCCTAAAACATGAAAA+CGG - chr1_3:65095688-65095707 None:intergenic 30.0%
CAATGACAAGATATATACCA+CGG - chr1_3:65095852-65095871 None:intergenic 30.0%
TAGCATATGAAGTGCATAAA+GGG - chr1_3:65095888-65095907 None:intergenic 30.0%
TTAGCATATGAAGTGCATAA+AGG - chr1_3:65095889-65095908 None:intergenic 30.0%
ACTTCATATGCTAACATTAC+AGG + chr1_3:65095895-65095914 Msa0133760:intron 30.0%
TTGAATGTGACAAAATATGG+GGG + chr1_3:65096281-65096300 Msa0133760:intron 30.0%
GAACAACTAGAGAAGAAAAA+GGG + chr1_3:65096339-65096358 Msa0133760:CDS 30.0%
AAAAGACCAAAGAGTTTCTT+TGG - chr1_3:65096510-65096529 None:intergenic 30.0%
! TGTTTTCTTCATGTGTGATT+TGG + chr1_3:65096589-65096608 Msa0133760:three_prime_UTR 30.0%
!! TTACAAGTGTTGGAGAGTTA+GGG - chr1_3:65094268-65094287 None:intergenic 35.0%
!! ATTACAAGTGTTGGAGAGTT+AGG - chr1_3:65094269-65094288 None:intergenic 35.0%
! TTTTCTCAGTTTCCATTTCC+TGG + chr1_3:65095289-65095308 Msa0133760:intron 35.0%
GATTGTAGTTGGATGGTAAT+TGG - chr1_3:65095372-65095391 None:intergenic 35.0%
GGGTAGGTTTAAAGAAATCA+AGG - chr1_3:65095454-65095473 None:intergenic 35.0%
TTAATGCATGTTTGGATGCA+CGG - chr1_3:65095481-65095500 None:intergenic 35.0%
TCACTTATAAACTCGTGTTC+AGG - chr1_3:65095604-65095623 None:intergenic 35.0%
!! ACTGATAAAACCGGTTTGTA+AGG - chr1_3:65095639-65095658 None:intergenic 35.0%
CGAACTCAAACTGATAAAAC+CGG - chr1_3:65095648-65095667 None:intergenic 35.0%
!! CGGTTTTATCAGTTTGAGTT+CGG + chr1_3:65095646-65095665 Msa0133760:intron 35.0%
TATGGTGACTCGATGTAATA+AGG - chr1_3:65096093-65096112 None:intergenic 35.0%
ATCGAGTCACCATAATTCTA+AGG + chr1_3:65096099-65096118 Msa0133760:intron 35.0%
AACTTTCATCCATAACAGCA+TGG + chr1_3:65096180-65096199 Msa0133760:CDS 35.0%
ACTTTCATCCATAACAGCAT+GGG + chr1_3:65096181-65096200 Msa0133760:CDS 35.0%
AGCTGAATTGAGAAAACTTG+AGG + chr1_3:65096229-65096248 Msa0133760:CDS 35.0%
GGAACAACTAGAGAAGAAAA+AGG + chr1_3:65096338-65096357 Msa0133760:intron 35.0%
AGAAAAGAAGGCTATGATTG+AGG + chr1_3:65096416-65096435 Msa0133760:CDS 35.0%
AGAATTACTCAAAGCTGAAG+AGG + chr1_3:65096452-65096471 Msa0133760:CDS 35.0%
TGCTACAATGTTCAAGATTG+TGG + chr1_3:65096534-65096553 Msa0133760:three_prime_UTR 35.0%
ACTCACTCATTCTTAGATGT+TGG + chr1_3:65096754-65096773 Msa0133760:three_prime_UTR 35.0%
ACTAAAAGTCTTAGCTGGGT+GGG - chr1_3:65094172-65094191 None:intergenic 40.0%
TACTAAAAGTCTTAGCTGGG+TGG - chr1_3:65094173-65094192 None:intergenic 40.0%
TCCAAAGAAAGATGTAGCTG+AGG + chr1_3:65094522-65094541 Msa0133760:CDS 40.0%
TCCTCAGCTACATCTTTCTT+TGG - chr1_3:65094526-65094545 None:intergenic 40.0%
GGAGGAGGATTATTATCTTG+TGG - chr1_3:65094562-65094581 None:intergenic 40.0%
TGTCCTATTGAGTTTCCTGA+AGG + chr1_3:65095151-65095170 Msa0133760:intron 40.0%
GTCCTATTGAGTTTCCTGAA+GGG + chr1_3:65095152-65095171 Msa0133760:intron 40.0%
TCCTATTGAGTTTCCTGAAG+GGG + chr1_3:65095153-65095172 Msa0133760:intron 40.0%
ATGTTTGGATGCACGGTATT+GGG - chr1_3:65095474-65095493 None:intergenic 40.0%
!! TGGTCTTGTAATCAGTGCAT+TGG + chr1_3:65095578-65095597 Msa0133760:intron 40.0%
!! TAAAACCGGTTTGTAAGGTG+AGG - chr1_3:65095634-65095653 None:intergenic 40.0%
TGCTGAAGAGAAACCAAAAG+AGG + chr1_3:65095813-65095832 Msa0133760:CDS 40.0%
GCTGAAGAGAAACCAAAAGA+GGG + chr1_3:65095814-65095833 Msa0133760:CDS 40.0%
CAAGAGTTGCAACAGAAAAG+AGG + chr1_3:65095954-65095973 Msa0133760:CDS 40.0%
GCTGTCACTAATCAAAGCAT+GGG + chr1_3:65095976-65095995 Msa0133760:CDS 40.0%
CATTAAGCATTAGATGGTGC+AGG - chr1_3:65096040-65096059 None:intergenic 40.0%
CAAAATAGCAGCACTTCACA+AGG + chr1_3:65096386-65096405 Msa0133760:CDS 40.0%
GCTATGATTGAGGCAAAGAA+AGG + chr1_3:65096426-65096445 Msa0133760:CDS 40.0%
AAGAGGTAGCTGCAAAATAC+AGG + chr1_3:65096469-65096488 Msa0133760:CDS 40.0%
AGAGGTAGCTGCAAAATACA+GGG + chr1_3:65096470-65096489 Msa0133760:CDS 40.0%
ACAGCTCCAAAGAAACTCTT+TGG + chr1_3:65096501-65096520 Msa0133760:CDS 40.0%
GAGTCAGTGGTCTAATACAA+AGG - chr1_3:65096738-65096757 None:intergenic 40.0%
TCTAAGAATGAGTGAGTCAG+TGG - chr1_3:65096751-65096770 None:intergenic 40.0%
TGGTAGGTACTCTTGTGAGT+TGG - chr1_3:65094144-65094163 None:intergenic 45.0%
! TCTTAGCTGGGTGGGTTTAT+TGG - chr1_3:65094164-65094183 None:intergenic 45.0%
!! AAGTGTTGGAGAGTTAGGGT+TGG - chr1_3:65094264-65094283 None:intergenic 45.0%
!! CATCTTTCTTTGGAGCCTCA+GGG - chr1_3:65094516-65094535 None:intergenic 45.0%
!! ACATCTTTCTTTGGAGCCTC+AGG - chr1_3:65094517-65094536 None:intergenic 45.0%
TTGGAGTCATCAACAACAGG+AGG - chr1_3:65094586-65094605 None:intergenic 45.0%
!! GCTTTGGAGTCATCAACAAC+AGG - chr1_3:65094589-65094608 None:intergenic 45.0%
CTGAGCTATGACAAGAGCTT+TGG - chr1_3:65094605-65094624 None:intergenic 45.0%
CCTATTGAGTTTCCTGAAGG+GGG + chr1_3:65095154-65095173 Msa0133760:intron 45.0%
CTATTGAGTTTCCTGAAGGG+GGG + chr1_3:65095155-65095174 Msa0133760:intron 45.0%
TATTGAGTTTCCTGAAGGGG+GGG + chr1_3:65095156-65095175 Msa0133760:intron 45.0%
ATTTCAATAACCCCCCCTTC+AGG - chr1_3:65095169-65095188 None:intergenic 45.0%
CATGTTTGGATGCACGGTAT+TGG - chr1_3:65095475-65095494 None:intergenic 45.0%
CGAATCCTCACCTTACAAAC+CGG + chr1_3:65095626-65095645 Msa0133760:intron 45.0%
ATTATACGCGGTCGATGTGA+AGG - chr1_3:65095739-65095758 None:intergenic 45.0%
GAAGAGAAACCAAAAGAGGG+CGG + chr1_3:65095817-65095836 Msa0133760:CDS 45.0%
GGCTGTCACTAATCAAAGCA+TGG + chr1_3:65095975-65095994 Msa0133760:CDS 45.0%
ACTGTTCTCCCATGCTGTTA+TGG - chr1_3:65096192-65096211 None:intergenic 45.0%
CAAGGCAGCTGAAGAAAAGA+AGG + chr1_3:65096404-65096423 Msa0133760:CDS 45.0%
! AGCTGGGTGGGTTTATTGGT+AGG - chr1_3:65094160-65094179 None:intergenic 50.0%
GTAGTCGTCTCAGTTGAAGC+AGG - chr1_3:65094472-65094491 None:intergenic 50.0%
TCATCAACAACAGGAGGAGG+AGG - chr1_3:65094580-65094599 None:intergenic 50.0%
GAGTCATCAACAACAGGAGG+AGG - chr1_3:65094583-65094602 None:intergenic 50.0%
CCCCCTTCAGGAAACTCAAT+AGG - chr1_3:65095157-65095176 None:intergenic 50.0%
! TTGGATGCACGGTATTGGGT+AGG - chr1_3:65095470-65095489 None:intergenic 50.0%
GCTGCAAAATACAGGGCAAC+TGG + chr1_3:65096477-65096496 Msa0133760:CDS 50.0%
GTTGGCACAGCGCACCAATA+GGG - chr1_3:65094126-65094145 None:intergenic 55.0%
AGTTGGCACAGCGCACCAAT+AGG - chr1_3:65094127-65094146 None:intergenic 55.0%
! TCAGTTGAAGCAGGTGGTGG+TGG - chr1_3:65094463-65094482 None:intergenic 55.0%
GTCTCAGTTGAAGCAGGTGG+TGG - chr1_3:65094466-65094485 None:intergenic 55.0%
GTCGTCTCAGTTGAAGCAGG+TGG - chr1_3:65094469-65094488 None:intergenic 55.0%
!! TTCTTTGGAGCCTCAGGGAG+AGG - chr1_3:65094511-65094530 None:intergenic 55.0%
TCAACAACAGGAGGAGGAGG+AGG - chr1_3:65094577-65094596 None:intergenic 55.0%
! TCGATAGAGCCGCCCTCTTT+TGG - chr1_3:65095829-65095848 None:intergenic 55.0%
!! GTTGAAGCAGGTGGTGGTGG+AGG - chr1_3:65094460-65094479 None:intergenic 60.0%
CTCAGGGAGAGGAGGAGAAG+GGG - chr1_3:65094500-65094519 None:intergenic 60.0%
CCTCAGGGAGAGGAGGAGAA+GGG - chr1_3:65094501-65094520 None:intergenic 60.0%
! TTTGGAGCCTCAGGGAGAGG+AGG - chr1_3:65094508-65094527 None:intergenic 60.0%
CCCTTCTCCTCCTCTCCCTG+AGG + chr1_3:65094498-65094517 Msa0133760:CDS 65.0%
GCCTCAGGGAGAGGAGGAGA+AGG - chr1_3:65094502-65094521 None:intergenic 65.0%
GAGGGCGGCTCTATCGACCG+TGG + chr1_3:65095832-65095851 Msa0133760:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 65094117 65096792 65094117 ID=Msa0133760;Name=Msa0133760
chr1_3 mRNA 65094117 65096792 65094117 ID=Msa0133760-mRNA-1;Parent=Msa0133760;Name=Msa0133760-mRNA-1;_AED=0.06;_eAED=0.06;_QI=289|1|1|1|1|1|5|259|212
chr1_3 exon 65094117 65094625 65094117 ID=Msa0133760-mRNA-1:exon:22072;Parent=Msa0133760-mRNA-1
chr1_3 exon 65095800 65095853 65095800 ID=Msa0133760-mRNA-1:exon:22073;Parent=Msa0133760-mRNA-1
chr1_3 exon 65095942 65096029 65095942 ID=Msa0133760-mRNA-1:exon:22074;Parent=Msa0133760-mRNA-1
chr1_3 exon 65096169 65096250 65096169 ID=Msa0133760-mRNA-1:exon:22075;Parent=Msa0133760-mRNA-1
chr1_3 exon 65096339 65096792 65096339 ID=Msa0133760-mRNA-1:exon:22076;Parent=Msa0133760-mRNA-1
chr1_3 five_prime_UTR 65094117 65094405 65094117 ID=Msa0133760-mRNA-1:five_prime_utr;Parent=Msa0133760-mRNA-1
chr1_3 CDS 65094406 65094625 65094406 ID=Msa0133760-mRNA-1:cds;Parent=Msa0133760-mRNA-1
chr1_3 CDS 65095800 65095853 65095800 ID=Msa0133760-mRNA-1:cds;Parent=Msa0133760-mRNA-1
chr1_3 CDS 65095942 65096029 65095942 ID=Msa0133760-mRNA-1:cds;Parent=Msa0133760-mRNA-1
chr1_3 CDS 65096169 65096250 65096169 ID=Msa0133760-mRNA-1:cds;Parent=Msa0133760-mRNA-1
chr1_3 CDS 65096339 65096533 65096339 ID=Msa0133760-mRNA-1:cds;Parent=Msa0133760-mRNA-1
chr1_3 three_prime_UTR 65096534 65096792 65096534 ID=Msa0133760-mRNA-1:three_prime_utr;Parent=Msa0133760-mRNA-1
Gene Sequence

>Msa0133760

ATGACAGATCAAGAGCAACCAAAAAAGATAGAATCTGAATCAAAATCGAATCCTCCACCACCACCTGCTTCAACTGAGACGACTACTACTACCCCTTCTCCTCCTCTCCCTGAGGCTCCAAAGAAAGATGTAGCTGAGGAGAAAGCTGTAATTCCACAAGATAATAATCCTCCTCCTCCTCCTGTTGTTGATGACTCCAAAGCTCTTGTCATAGCTCAGAAGACTGATGAAGTTGCTGAAGAGAAACCAAAAGAGGGCGGCTCTATCGACCGTGATGCTGTGCTTACAAGAGTTGCAACAGAAAAGAGGCTGTCACTAATCAAAGCATGGGAAGAAAGTGAAAAGTCAAAAGCAGAGAACAAAGCTCAGAGAAAACTTTCATCCATAACAGCATGGGAGAACAGTAAGAAAGCTGCTAAAGAAGCTGAATTGAGAAAACTTGAGGAACAACTAGAGAAGAAAAAGGGAGAATATGCTGAGAAAATGAAAAACAAAATAGCAGCACTTCACAAGGCAGCTGAAGAAAAGAAGGCTATGATTGAGGCAAAGAAAGGTGAAGAATTACTCAAAGCTGAAGAGGTAGCTGCAAAATACAGGGCAACTGGAACAGCTCCAAAGAAACTCTTTGGTCTTTTCTAA
Protein sequence

>Msa0133760

MTDQEQPKKIESESKSNPPPPPASTETTTTTPSPPLPEAPKKDVAEEKAVIPQDNNPPPPPVVDDSKALVIAQKTDEVAEEKPKEGGSIDRDAVLTRVATEKRLSLIKAWEESEKSKAENKAQRKLSSITAWENSKKAAKEAELRKLEEQLEKKKGEYAEKMKNKIAALHKAAEEKKAMIEAKKGEELLKAEEVAAKYRATGTAPKKLFGLF*