AlfalfaGEDB | Msa0134440

M. sativa cultivar zhongmu-4 / Msa0134440

KEGG: K09419
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134440	XP_013469753.1	93.939	330	16	1	1	326	1	330	0.0	635

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134440	sp\|Q9LUH8\|HFA6B_ARATH	52.078	361	113	6	22	323	43	402	2.37e-118	350

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134440	A0A072VQ54	93.939	330	16	1	1	326	1	330	0.0	635

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TFs/TRs:

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Gene ID	Type	Classification
Msa0134440	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0040140	Msa0134440	0.832641	8.416219e-56	-8.615850e-47
Msa0087850	Msa0134440	0.925806	1.112849e-90	-8.615850e-47
Msa0134440	Msa0180560	0.855560	5.811341e-62	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134440	MtrunA17_Chr1g0204741	93.939	330	16	1	1	326	1	330	0.0	635
Msa0134440	MtrunA17_Chr7g0260331	53.274	336	126	6	17	323	26	359	6.83e-109	321
Msa0134440	MtrunA17_Chr3g0135831	62.963	216	73	4	27	240	33	243	5.99e-91	276
Msa0134440	MtrunA17_Chr5g0410881	58.296	223	81	4	27	239	19	239	1.21e-81	256
Msa0134440	MtrunA17_Chr1g0185131	48.710	310	123	8	23	307	15	313	6.41e-76	236
Msa0134440	MtrunA17_Chr4g0039891	56.190	210	81	4	39	240	11	217	1.61e-71	229
Msa0134440	MtrunA17_Chr4g0071051	47.541	244	113	5	12	250	73	306	2.85e-69	222
Msa0134440	MtrunA17_Chr8g0379701	38.408	289	158	5	39	318	11	288	2.96e-57	190
Msa0134440	MtrunA17_Chr8g0392001	49.505	202	84	6	40	227	22	219	1.93e-52	176
Msa0134440	MtrunA17_Chr6g0485571	43.627	204	108	3	40	243	11	207	4.01e-52	175

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134440	AT3G22830.1	52.078	361	113	6	22	323	43	402	2.41e-119	350
Msa0134440	AT2G26150.1	43.252	326	146	9	27	324	31	345	1.42e-85	261
Msa0134440	AT2G26150.4	43.252	326	146	9	27	324	31	345	1.42e-85	261
Msa0134440	AT3G51910.1	57.820	211	74	3	31	240	20	216	1.98e-81	248
Msa0134440	AT4G17750.1	53.012	249	91	6	16	240	22	268	1.86e-77	245
Msa0134440	AT5G16820.1	58.065	217	82	3	36	244	23	238	8.86e-77	243
Msa0134440	AT5G16820.2	58.065	217	82	3	36	244	23	238	8.86e-77	243
Msa0134440	AT1G32330.2	53.333	240	95	4	16	239	11	249	8.45e-76	241
Msa0134440	AT1G32330.1	53.333	240	95	4	16	239	11	249	1.01e-75	241
Msa0134440	AT5G43840.1	57.732	194	69	1	27	220	6	186	3.22e-75	233

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Find 68 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGATGATGAATTTCA+TGG	0.398561	1_3:-65945797	Msa0134440:CDS
AAAAGGTTGATGCAGATAGG+TGG	0.546223	1_3:-65946092	Msa0134440:CDS
AAAGGTTGATGCAGATAGGT+GGG	0.494489	1_3:-65946091	Msa0134440:CDS
AATATGAATCTTCATCATGG+TGG	0.612258	1_3:-65947078	Msa0134440:CDS
ACAAGTTCTAATGGTTGAGT+TGG	0.523658	1_3:-65945902	Msa0134440:CDS
ACATCTGCATCAACATTACT+AGG	0.447700	1_3:+65945661	None:intergenic
ACTAACTGTTGCAAGAAATT+AGG	0.251206	1_3:+65945757	None:intergenic
AGAAGGAGAACTATTGACGA+AGG	0.628728	1_3:-65945685	Msa0134440:CDS
AGAATAATAGAAAAGGCTTG+AGG	0.598445	1_3:+65946868	None:intergenic
AGACCCGTCATGTGTTGAGC+TGG	0.495058	1_3:-65945974	Msa0134440:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATATGAATCTTCATCATGG+TGG	-	chr1_3:65945441-65945460	Msa0134440:CDS	30.0%
ATATGAATCTTCATCATGGT+GGG	-	chr1_3:65945442-65945461	Msa0134440:CDS	30.0%
CCATGAAACTATATGATTTG+TGG	+	chr1_3:65945598-65945617	None:intergenic	30.0%
CCACAAATCATATAGTTTCA+TGG	-	chr1_3:65945595-65945614	Msa0134440:CDS	30.0%
AGAATAATAGAAAAGGCTTG+AGG	+	chr1_3:65945654-65945673	None:intergenic	30.0%
AGGGAGAAGAATAATAGAAA+AGG	+	chr1_3:65945661-65945680	None:intergenic	30.0%
AAAGTCAAAATGTATTGGAC+GGG	-	chr1_3:65946030-65946049	Msa0134440:CDS	30.0%
AAACACGACATTGATACATT+TGG	-	chr1_3:65946225-65946244	Msa0134440:intron	30.0%
ACTAACTGTTGCAAGAAATT+AGG	+	chr1_3:65946765-65946784	None:intergenic	30.0%
AATATGAATCTTCATCATGG+TGG	-	chr1_3:65945441-65945460	Msa0134440:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	65945438	65947103	65945438	ID=Msa0134440;Name=Msa0134440
chr1_3	mRNA	65945438	65947103	65945438	ID=Msa0134440-mRNA-1;Parent=Msa0134440;Name=Msa0134440-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|0\|0\|1\|1\|1\|2\|0\|326
chr1_3	exon	65946807	65947103	65946807	ID=Msa0134440-mRNA-1:exon:22452;Parent=Msa0134440-mRNA-1
chr1_3	exon	65945438	65946121	65945438	ID=Msa0134440-mRNA-1:exon:22451;Parent=Msa0134440-mRNA-1
chr1_3	CDS	65946807	65947103	65946807	ID=Msa0134440-mRNA-1:cds;Parent=Msa0134440-mRNA-1
chr1_3	CDS	65945438	65946121	65945438	ID=Msa0134440-mRNA-1:cds;Parent=Msa0134440-mRNA-1

Gene Sequence

>Msa0134440

ATGAATATGAATCTTCATCATGGTGGGATGAAGGAGGAGTTTGTTGGAGGAAGTTCTTCCTTTTCTGTGGTTGGTGAACCTCAACCAAGGGAAGGACTTCATGAAAATGGTCCTCCACCATTCCTCACAAAAACATATGATATAGTTGATGATCCTTCCACAAATCATATAGTTTCATGGAGTACTGGGAACAATAGCTTTGTTGTTTGGGATCCTCAAGCCTTTTCTATTATTCTTCTCCCTAGATTCTTCAAGCACAACAATTTCTCCAGTTTTGTCCGGCAACTTAATACCTATGGGTTTAAAAAGGTTGATGCAGATAGGTGGGAATTTGCTAATGAGATGTTTCTAAGAGGACAAAAAATCCTTTTGAAGAACATTAGAAGAAGAAAAACAAATCAATCTCAAGTAATGCAAGCACTAGACCCGTCATGTGTTGAGCTGGGAAGGTTTGGAGTAGACGGAATCGAAGTGGACCGGTTGAGGCGCGACAGACAAGTTCTAATGGTTGAGTTGGTGAAACTTAGACAACAACAGCAGAATACTAGAACTCATCTTGAAGCAATGGAAAGAAGGCTTAAAAGAAGTGAACAGAAACAAAAACAGATGATGAATTTCATGGCAAGGGCAATACAAAAGCCTAATTTCTTGCAACAGTTAGTTCAGCAGAAGGAATGGAGAAAAGAGTTAGAGGAAATTTTTTCTAAGAAGAGAAGGAGAACTATTGACGAAGGGCCTAGTAATGTTGATGCAGATGTTGAATTAGGATATGTTGATAAAGATTGCTTAAATTTTGTCAAACTTGAACAACATCAAGATGTTGAGGAAAACTCCAATCAAATTAATCAACTTGAAGTAAGAGATACTGAAGTGTTTTGGCAAGATTTGTTTAAGGAGGGTATTGAAGATGAAATAGATATTGAAGATGTTGATGTATTGGCTGAGCAACTTGGTTATCTTGCCTCTAGTCCTCCTAAATAG

Protein sequence

>Msa0134440

MNMNLHHGGMKEEFVGGSSSFSVVGEPQPREGLHENGPPPFLTKTYDIVDDPSTNHIVSWSTGNNSFVVWDPQAFSIILLPRFFKHNNFSSFVRQLNTYGFKKVDADRWEFANEMFLRGQKILLKNIRRRKTNQSQVMQALDPSCVELGRFGVDGIEVDRLRRDRQVLMVELVKLRQQQQNTRTHLEAMERRLKRSEQKQKQMMNFMARAIQKPNFLQQLVQQKEWRKELEEIFSKKRRRTIDEGPSNVDADVELGYVDKDCLNFVKLEQHQDVEENSNQINQLEVRDTEVFWQDLFKEGIEDEIDIEDVDVLAEQLGYLASSPPK*