AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0139690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139690 MtrunA17_Chr8g0344921 99.160 119 1 0 1 119 1 119 2.01e-87 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139690 AT3G59650.1 83.193 119 20 0 1 119 1 119 7.54e-74 215
Msa0139690 AT3G59650.2 67.808 146 20 1 1 119 1 146 5.08e-68 201

Find 68 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTGATAGGGCATTTA+TGG 0.120191 1_3:-73517097 Msa0139690:intron
GTCGCATTTGCCAGCGTTTA+AGG 0.191698 1_3:-73517073 Msa0139690:CDS
CACACACCACTCGGTCATTA+TGG 0.253183 1_3:+73516892 None:intergenic
GTGAGCTGTGCGGCGGCGTT+AGG 0.286266 1_3:+73519266 None:intergenic
GTAACTATGAATTATTTCTT+TGG 0.303962 1_3:+73516403 None:intergenic
GACAACTGCTTTGAAATATT+AGG 0.339185 1_3:-73516745 Msa0139690:exon
AGATGGCATTGCGAGGAGTT+TGG 0.367673 1_3:-73519193 Msa0139690:CDS
CGTTGTTAGCTATAGCGATT+GGG 0.368754 1_3:-73519156 Msa0139690:CDS
TCCCAGTGCATTCCTTAGCC+TGG 0.373420 1_3:+73516830 None:intergenic
AATTCATAGTTACTATATAA+TGG 0.374235 1_3:-73516393 Msa0139690:three_prime_UTR
AGTGGTGTGTGTGAAGAATA+TGG 0.393785 1_3:-73516880 Msa0139690:CDS
AAAAGGTAGAAACTATATAA+TGG 0.394205 1_3:-73516352 Msa0139690:three_prime_UTR
ACCTTGCACGCTAGGGTGTT+TGG 0.399445 1_3:+73516773 None:intergenic
AAATTCTCAATCGGAGAAAA+CGG 0.414689 1_3:+73519319 None:intergenic
TGAGCTGTGCGGCGGCGTTA+GGG 0.416592 1_3:+73519267 None:intergenic
TGTTGTTTGGCGCTATTGCC+AGG 0.418369 1_3:-73516628 Msa0139690:three_prime_UTR
GCTTAAGTTCTCATGTTGTT+TGG 0.421272 1_3:-73516641 Msa0139690:three_prime_UTR
TTAATCAAATATCTGTGAAC+TGG 0.439984 1_3:-73516667 Msa0139690:three_prime_UTR
GTCCATGTACCTTGCACGCT+AGG 0.449503 1_3:+73516765 None:intergenic
GTGGAAGTAGCAGAGGCATT+AGG 0.465860 1_3:-73519133 Msa0139690:intron
GCATGCAGAAGTATCTCTTC+CGG 0.466200 1_3:+73516855 None:intergenic
GGAGGCGAACCCTCAGTTAG+AGG 0.466422 1_3:-73517052 Msa0139690:CDS
GTATGTATTAGTCAACAAAA+AGG 0.475742 1_3:-73516369 Msa0139690:three_prime_UTR
AATTCTCAATCGGAGAAAAC+GGG 0.485540 1_3:+73519320 None:intergenic
TGATTAACTGACCAGAGAAA+AGG 0.500162 1_3:+73516683 None:intergenic
ATCTGATGAATGGTGGTTGT+TGG 0.508884 1_3:+73519212 None:intergenic
ACCCTAGCGTGCAAGGTACA+TGG 0.511271 1_3:-73516767 Msa0139690:CDS
TCGTTGTTAGCTATAGCGAT+TGG 0.511918 1_3:-73519157 Msa0139690:CDS
TGGTCAGCATCCACATCTAA+AGG 0.514097 1_3:-73517010 Msa0139690:CDS
TTCTGCATGCAACCAGGCTA+AGG 0.518615 1_3:-73516842 Msa0139690:CDS
AACCAGGCTAAGGAATGCAC+TGG 0.528014 1_3:-73516832 Msa0139690:CDS
TCGCAATGCCATCTGATGAA+TGG 0.539991 1_3:+73519202 None:intergenic
TACAAACTAACAATATATAA+CGG 0.542968 1_3:+73516516 None:intergenic
ATGGTGTTTAACTGAAATCC+TGG 0.546587 1_3:+73516610 None:intergenic
GTTGGACGGCTGACGGTGAT+CGG 0.546998 1_3:+73519230 None:intergenic
GGGTTCGCCTCCTTAAACGC+TGG 0.551134 1_3:+73517063 None:intergenic
TCCATGTACCTTGCACGCTA+GGG 0.551964 1_3:+73516766 None:intergenic
GATTAACTATCAGCTGATTG+TGG 0.553497 1_3:-73516562 Msa0139690:three_prime_UTR
CGGCTGACGGTGATCGGAGA+CGG 0.561368 1_3:+73519236 None:intergenic
ACCAAACACCCTAGCGTGCA+AGG 0.562815 1_3:-73516774 Msa0139690:CDS
TCAGTGTCCACCTCTAACTG+AGG 0.586148 1_3:+73517042 None:intergenic
ACACTTAAAAGACTAAGCTA+TGG 0.587092 1_3:+73516591 None:intergenic
GTGTGTGAAGAATATGGATC+CGG 0.590195 1_3:-73516874 Msa0139690:CDS
GGCTAAGGAATGCACTGGGA+AGG 0.590269 1_3:-73516827 Msa0139690:CDS
AACAACCACCATTCATCAGA+TGG 0.591625 1_3:-73519210 Msa0139690:exon
GTGGACACTGAAATGATTCG+TGG 0.592382 1_3:-73517030 Msa0139690:CDS
GATGAATGGTGGTTGTTGGA+CGG 0.594620 1_3:+73519216 None:intergenic
AGATACTTCTGCATGCAACC+AGG 0.597112 1_3:-73516848 Msa0139690:CDS
CAGTGTCCACCTCTAACTGA+GGG 0.607705 1_3:+73517043 None:intergenic
GGATCGACTTAGCACTACTA+CGG 0.608740 1_3:+73516704 None:intergenic
TATTCTTCACACACACCACT+CGG 0.614073 1_3:+73516883 None:intergenic
GGCGAACCCTCAGTTAGAGG+TGG 0.624239 1_3:-73517049 Msa0139690:CDS
GGTGGTTGTTGGACGGCTGA+CGG 0.631122 1_3:+73519223 None:intergenic
TTTCTTTGGCGAGTAAACCA+TGG 0.636878 1_3:+73516417 None:intergenic
TTATCTTCGCTAGAGAACCA+TGG 0.637249 1_3:-73516434 Msa0139690:three_prime_UTR
GTTGTTAGCTATAGCGATTG+GGG 0.643269 1_3:-73519155 Msa0139690:CDS
GCATTTGCCAGCGTTTAAGG+AGG 0.645640 1_3:-73517070 Msa0139690:CDS
GAAGGAAAGTAATTAAGCTG+AGG 0.646557 1_3:-73516809 Msa0139690:CDS
ACCAGGCTAAGGAATGCACT+GGG 0.650150 1_3:-73516831 Msa0139690:CDS
AGGCTCTGTGAGCTGTGCGG+CGG 0.653718 1_3:+73519259 None:intergenic
ATTCATCAGATGGCATTGCG+AGG 0.654895 1_3:-73519200 Msa0139690:exon
GATTGGGGTGGAAGTAGCAG+AGG 0.657242 1_3:-73519140 Msa0139690:CDS
GTTAGCTATAGCGATTGGGG+TGG 0.672048 1_3:-73519152 Msa0139690:CDS
TATAACGGAGTACACAAACT+CGG 0.675147 1_3:+73516531 None:intergenic
CAATGCCATCTGATGAATGG+TGG 0.680972 1_3:+73519205 None:intergenic
CTGACGGTGATCGGAGACGG+AGG 0.714407 1_3:+73519239 None:intergenic
CGGAGGCTCTGTGAGCTGTG+CGG 0.730038 1_3:+73519256 None:intergenic
AGAGAACCATAATGACCGAG+TGG 0.735330 1_3:-73516898 Msa0139690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAATTAAAAGAGT+TGG - chr1_3:73516452-73516471 Msa0139690:three_prime_UTR 10.0%
!!! TATTTTTAATTAAAAGAGTT+GGG - chr1_3:73516453-73516472 Msa0139690:three_prime_UTR 10.0%
!! ATAAAAAAATGAAAACTTTA+GGG + chr1_3:73516770-73516789 None:intergenic 10.0%
!! AATAAAAAAATGAAAACTTT+AGG + chr1_3:73516771-73516790 None:intergenic 10.0%
!!! AAAGTTTTCATTTTTTTATT+TGG - chr1_3:73516771-73516790 Msa0139690:CDS 10.0%
!!! AAGTTTTCATTTTTTTATTT+GGG - chr1_3:73516772-73516791 Msa0139690:CDS 10.0%
!!! AATTTTATGAATTATGATAT+TGG - chr1_3:73517371-73517390 Msa0139690:intron 10.0%
!!! TTTTTAAAAAACTGTTTTTA+TGG - chr1_3:73517613-73517632 Msa0139690:intron 10.0%
!!! TTTTTTTAAAACTGTTTTTA+TGG - chr1_3:73518408-73518427 Msa0139690:intron 10.0%
!!! TTTAGTTTTAAATATTTGAT+AGG - chr1_3:73518699-73518718 Msa0139690:intron 10.0%
!!! TTAGTTTTAAATATTTGATA+GGG - chr1_3:73517897-73517916 Msa0139690:intron 10.0%
!!! ATTTTTTTATTTGGGTATTT+AGG - chr1_3:73516780-73516799 Msa0139690:CDS 15.0%
!! AAATCAAACAGATATAATAT+TGG - chr1_3:73517297-73517316 Msa0139690:intron 15.0%
!!! TTTTTTAAAAATTTCTGCAT+CGG + chr1_3:73517604-73517623 None:intergenic 15.0%
!!! CTTAGTTTTAAATATTTGAT+AGG - chr1_3:73517896-73517915 Msa0139690:intron 15.0%
!!! AAATGTTTAGAATGTTATTT+TGG + chr1_3:73518216-73518235 None:intergenic 15.0%
!! TACAAACTAACAATATATAA+CGG + chr1_3:73519293-73519312 None:intergenic 15.0%
!!! GATAAAAAAAAAAAGTACAA+AGG + chr1_3:73519357-73519376 None:intergenic 15.0%
!! AATTCATAGTTACTATATAA+TGG - chr1_3:73519413-73519432 Msa0139690:five_prime_UTR 15.0%
!! GAAAAATGAAAGAATGTAAA+GGG + chr1_3:73516714-73516733 None:intergenic 20.0%
!! TGAAAAATGAAAGAATGTAA+AGG + chr1_3:73516715-73516734 None:intergenic 20.0%
!!! GAAATCATTTAAAATCTGAA+TGG - chr1_3:73517489-73517508 Msa0139690:intron 20.0%
!!! GTAACTATGAATTATTTCTT+TGG + chr1_3:73519406-73519425 None:intergenic 20.0%
!!! GATTTTGAGAAATTCTCAAT+CGG + chr1_3:73516499-73516518 None:intergenic 25.0%
!!! TTAGGTTTGTTGAATTATTG+TGG - chr1_3:73516798-73516817 Msa0139690:CDS 25.0%
!!! TCTTGCTTTTATTCAAATAG+TGG - chr1_3:73516837-73516856 Msa0139690:CDS 25.0%
!! CAAATTGCTATTGTATTGTT+CGG + chr1_3:73517011-73517030 None:intergenic 25.0%
!!! TTTTTGTTTTGCTAGTTACA+AGG - chr1_3:73517114-73517133 Msa0139690:intron 25.0%
! GTTTCTAATTCTAAATGTCT+AGG - chr1_3:73517170-73517189 Msa0139690:intron 25.0%
! TTAAAAGGTCTGAAAAGAAT+AGG + chr1_3:73517245-73517264 None:intergenic 25.0%
!!! TGATATTGGTGATATTTTCT+TGG - chr1_3:73517385-73517404 Msa0139690:intron 25.0%
!!! AAAATAGTTTCTTTTTTCCC+TGG - chr1_3:73517460-73517479 Msa0139690:intron 25.0%
! TAAATGATTTCAAAACTCCA+GGG + chr1_3:73517480-73517499 None:intergenic 25.0%
! TTAAATGATTTCAAAACTCC+AGG + chr1_3:73517481-73517500 None:intergenic 25.0%
!!! AAAACTGTTTTTATGGTTTG+AGG - chr1_3:73517620-73517639 Msa0139690:intron 25.0%
! TTTGAGGACAATACTAATAA+TGG - chr1_3:73517636-73517655 Msa0139690:intron 25.0%
! ACTAATAATGGCATTGTAAA+TGG - chr1_3:73517648-73517667 Msa0139690:intron 25.0%
! AATATTTGATAGGCCATTTA+TGG - chr1_3:73517906-73517925 Msa0139690:intron 25.0%
!!! AAAATTGTTGCTTTTTTCCT+TGG - chr1_3:73518147-73518166 Msa0139690:intron 25.0%
! AAAATAGTATGCATCAGATT+CGG + chr1_3:73518385-73518404 None:intergenic 25.0%
! ATTGTTAAAAGGTTGTGATT+TGG + chr1_3:73518515-73518534 None:intergenic 25.0%
! TCTCGAATTTGATTGTTAAA+AGG + chr1_3:73518526-73518545 None:intergenic 25.0%
!! TGAATTTTACCTAGTACTAA+AGG - chr1_3:73518602-73518621 Msa0139690:intron 25.0%
!! AATATTTGATAGGGCATTTA+TGG - chr1_3:73518709-73518728 Msa0139690:intron 25.0%
!! CTTTTTACAGTAAGATTCTT+GGG - chr1_3:73518819-73518838 Msa0139690:intron 25.0%
! TTAATCAAATATCTGTGAAC+TGG - chr1_3:73519139-73519158 Msa0139690:CDS 25.0%
! GTATGTATTAGTCAACAAAA+AGG - chr1_3:73519437-73519456 Msa0139690:five_prime_UTR 25.0%
AAATTCTCAATCGGAGAAAA+CGG + chr1_3:73516490-73516509 None:intergenic 30.0%
GTTCGGTAATACACTATATT+CGG + chr1_3:73516994-73517013 None:intergenic 30.0%
TCTAGGTGATATGATAGTAT+AGG - chr1_3:73517187-73517206 Msa0139690:intron 30.0%
AAAAATTTCTGCATCGGATT+CGG + chr1_3:73517598-73517617 None:intergenic 30.0%
TCTCAGACTTGATTGTTAAA+AGG + chr1_3:73517730-73517749 None:intergenic 30.0%
! TGCATTTTACCTAGTACTAA+AGG - chr1_3:73517806-73517825 Msa0139690:intron 30.0%
TATTCATAGCATATTGTTCC+AGG + chr1_3:73517832-73517851 None:intergenic 30.0%
!! TATTCTCTCCTGTTTTGAAT+AGG - chr1_3:73517862-73517881 Msa0139690:intron 30.0%
AACAATCTCCTATTCAAAAC+AGG + chr1_3:73517873-73517892 None:intergenic 30.0%
!! TTTTTCAATGATTGACTCCT+AGG + chr1_3:73517964-73517983 None:intergenic 30.0%
! CTAGCAAGTATTAGTGATTT+TGG - chr1_3:73517985-73518004 Msa0139690:intron 30.0%
!!! TATTAGTGATTTTGGAGTCA+TGG - chr1_3:73517993-73518012 Msa0139690:intron 30.0%
!!! TGTTATTTTGGTTCTAGCAA+TGG + chr1_3:73518204-73518223 None:intergenic 30.0%
!! TCAGAGGCATTGAAAAAATA+TGG - chr1_3:73518302-73518321 Msa0139690:intron 30.0%
! TATTTTTCCTTTTGCCTGAT+TGG - chr1_3:73518470-73518489 Msa0139690:intron 30.0%
TAACAATCAAATTCGAGAGA+AGG - chr1_3:73518528-73518547 Msa0139690:intron 30.0%
AAGAATTCCCTATTCAAAAC+AGG + chr1_3:73518676-73518695 None:intergenic 30.0%
! GCTTTTTACAGTAAGATTCT+TGG - chr1_3:73518818-73518837 Msa0139690:intron 30.0%
! TGTAATTGATGATTCTCTTG+TGG - chr1_3:73518880-73518899 Msa0139690:intron 30.0%
!! GACAACTGCTTTGAAATATT+AGG - chr1_3:73519061-73519080 Msa0139690:intron 30.0%
ACACTTAAAAGACTAAGCTA+TGG + chr1_3:73519218-73519237 None:intergenic 30.0%
AATTCTCAATCGGAGAAAAC+GGG + chr1_3:73516489-73516508 None:intergenic 35.0%
ATCTCAATCATTATGTGCGT+GGG + chr1_3:73516875-73516894 None:intergenic 35.0%
AATCTCAATCATTATGTGCG+TGG + chr1_3:73516876-73516895 None:intergenic 35.0%
TTGATCAGTGTTAACGATAG+TGG - chr1_3:73516894-73516913 Msa0139690:CDS 35.0%
GCAATGCTATAGCTGTAAAT+TGG - chr1_3:73516956-73516975 Msa0139690:intron 35.0%
CAAATTTCAACTCGATTGCT+AGG - chr1_3:73517410-73517429 Msa0139690:intron 35.0%
TATTCTTCTTGTTGCCTGAT+TGG - chr1_3:73517675-73517694 Msa0139690:intron 35.0%
TAACAATCAAGTCTGAGAGA+AGG - chr1_3:73517732-73517751 Msa0139690:intron 35.0%
CTAAGAAGTCAGTAAAACCA+AGG + chr1_3:73518167-73518186 None:intergenic 35.0%
AGTCAAAGCAACTGAAAGAT+GGG + chr1_3:73518281-73518300 None:intergenic 35.0%
TAGTCAAAGCAACTGAAAGA+TGG + chr1_3:73518282-73518301 None:intergenic 35.0%
GCTAAGAATAACGTTGTAGA+TGG - chr1_3:73518443-73518462 Msa0139690:intron 35.0%
TCAGGCAAAAGGAAAAATAC+AGG + chr1_3:73518469-73518488 None:intergenic 35.0%
TTTACCTAGTACTAAAGGCT+CGG - chr1_3:73518607-73518626 Msa0139690:intron 35.0%
!! TATTCTCGCCTGTTTTGAAT+AGG - chr1_3:73518665-73518684 Msa0139690:intron 35.0%
!! ATTCTCGCCTGTTTTGAATA+GGG - chr1_3:73518666-73518685 Msa0139690:intron 35.0%
CTGTAAAAAGCCTTTAGATG+TGG + chr1_3:73518809-73518828 None:intergenic 35.0%
GAAGGAAAGTAATTAAGCTG+AGG - chr1_3:73518997-73519016 Msa0139690:intron 35.0%
TGATTAACTGACCAGAGAAA+AGG + chr1_3:73519126-73519145 None:intergenic 35.0%
GCTTAAGTTCTCATGTTGTT+TGG - chr1_3:73519165-73519184 Msa0139690:CDS 35.0%
ATGGTGTTTAACTGAAATCC+TGG + chr1_3:73519199-73519218 None:intergenic 35.0%
GATTAACTATCAGCTGATTG+TGG - chr1_3:73519244-73519263 Msa0139690:five_prime_UTR 35.0%
! TATAACGGAGTACACAAACT+CGG + chr1_3:73519278-73519297 None:intergenic 35.0%
ATCTGATGAATGGTGGTTGT+TGG + chr1_3:73516597-73516616 None:intergenic 40.0%
AACAACCACCATTCATCAGA+TGG - chr1_3:73516596-73516615 Msa0139690:three_prime_UTR 40.0%
TCGTTGTTAGCTATAGCGAT+TGG - chr1_3:73516649-73516668 Msa0139690:three_prime_UTR 40.0%
CGTTGTTAGCTATAGCGATT+GGG - chr1_3:73516650-73516669 Msa0139690:three_prime_UTR 40.0%
GTTGTTAGCTATAGCGATTG+GGG - chr1_3:73516651-73516670 Msa0139690:three_prime_UTR 40.0%
AATCATTATGTGCGTGGGTT+GGG + chr1_3:73516870-73516889 None:intergenic 40.0%
!! TCAGTGTTAACGATAGTGGA+TGG - chr1_3:73516898-73516917 Msa0139690:CDS 40.0%
! GCGGAAAATAGCGATTTGTT+TGG - chr1_3:73516920-73516939 Msa0139690:intron 40.0%
! TGTCAACGTCGGTGTTTAAA+AGG + chr1_3:73517260-73517279 None:intergenic 40.0%
ATTACTACCAGTGTCAACGT+CGG + chr1_3:73517271-73517290 None:intergenic 40.0%
! ATACCTTTGATCAGAAGTGC+CGG - chr1_3:73517324-73517343 Msa0139690:intron 40.0%
AATCAGAAGCTATGTAGCAC+CGG + chr1_3:73517346-73517365 None:intergenic 40.0%
AGTCAAAGCAACTGAAAGAC+GGG + chr1_3:73517516-73517535 None:intergenic 40.0%
CAGTCAAAGCAACTGAAAGA+CGG + chr1_3:73517517-73517536 None:intergenic 40.0%
! TTTCAGTTGCTTTGACTGAG+AGG - chr1_3:73517519-73517538 Msa0139690:intron 40.0%
! TTCAGTTGCTTTGACTGAGA+GGG - chr1_3:73517520-73517539 Msa0139690:intron 40.0%
TTTACCTAGTACTAAAGGCC+TGG - chr1_3:73517811-73517830 Msa0139690:intron 40.0%
TTGCCAGCCTAATAAGGTTT+AGG - chr1_3:73517937-73517956 Msa0139690:intron 40.0%
TGCCAGCCTAATAAGGTTTA+GGG - chr1_3:73517938-73517957 Msa0139690:intron 40.0%
! TCAGTTGCTTTGACTATCAG+AGG - chr1_3:73518286-73518305 Msa0139690:intron 40.0%
TTGCCACATCAGAATTGTTC+TGG + chr1_3:73518329-73518348 None:intergenic 40.0%
AGATTCGGAGTGTGATTACA+TGG + chr1_3:73518370-73518389 None:intergenic 40.0%
TTGGAAGTGACATGAAGTCA+CGG + chr1_3:73518496-73518515 None:intergenic 40.0%
! GACAATCAGAAGTAGCTTTG+TGG + chr1_3:73518580-73518599 None:intergenic 40.0%
TATTCTTCACACACACCACT+CGG + chr1_3:73518926-73518945 None:intergenic 40.0%
AGTGGTGTGTGTGAAGAATA+TGG - chr1_3:73518926-73518945 Msa0139690:intron 40.0%
GTGTGTGAAGAATATGGATC+CGG - chr1_3:73518932-73518951 Msa0139690:intron 40.0%
TTATCTTCGCTAGAGAACCA+TGG - chr1_3:73519372-73519391 Msa0139690:five_prime_UTR 40.0%
TTTCTTTGGCGAGTAAACCA+TGG + chr1_3:73519392-73519411 None:intergenic 40.0%
GATGAATGGTGGTTGTTGGA+CGG + chr1_3:73516593-73516612 None:intergenic 45.0%
CAATGCCATCTGATGAATGG+TGG + chr1_3:73516604-73516623 None:intergenic 45.0%
TCGCAATGCCATCTGATGAA+TGG + chr1_3:73516607-73516626 None:intergenic 45.0%
ATTCATCAGATGGCATTGCG+AGG - chr1_3:73516606-73516625 Msa0139690:three_prime_UTR 45.0%
CAATCATTATGTGCGTGGGT+TGG + chr1_3:73516871-73516890 None:intergenic 45.0%
!! GTGTTAACGATAGTGGATGG+CGG - chr1_3:73516901-73516920 Msa0139690:intron 45.0%
TTAAACACCGACGTTGACAC+TGG - chr1_3:73517261-73517280 Msa0139690:intron 45.0%
! TCAGTTGCTTTGACTGAGAG+GGG - chr1_3:73517521-73517540 Msa0139690:intron 45.0%
GGTCAAGAACAATTCTGCTG+TGG - chr1_3:73517557-73517576 Msa0139690:intron 45.0%
GAACAATTCTGCTGTGGCAA+CGG - chr1_3:73517563-73517582 Msa0139690:intron 45.0%
TGTTCCAGGCCTTTAGTACT+AGG + chr1_3:73517818-73517837 None:intergenic 45.0%
GGCAAATGCGACTCCATAAA+TGG + chr1_3:73517922-73517941 None:intergenic 45.0%
TCGCATTTGCCAGCCTAATA+AGG - chr1_3:73517931-73517950 Msa0139690:intron 45.0%
CCTAGGCCCTAAACCTTATT+AGG + chr1_3:73517947-73517966 None:intergenic 45.0%
! CCTAATAAGGTTTAGGGCCT+AGG - chr1_3:73517944-73517963 Msa0139690:intron 45.0%
GAAGCCACTTGAAACCTGAA+TGG - chr1_3:73518254-73518273 Msa0139690:intron 45.0%
ACTGAAAGATGGGTCCATTC+AGG + chr1_3:73518271-73518290 None:intergenic 45.0%
GGTCCAGAACAATTCTGATG+TGG - chr1_3:73518323-73518342 Msa0139690:intron 45.0%
ACTTCCGAGCCTTTAGTACT+AGG + chr1_3:73518614-73518633 None:intergenic 45.0%
AGTACTAAAGGCTCGGAAGT+TGG - chr1_3:73518614-73518633 Msa0139690:intron 45.0%
GTGGACACTGAAATGATTCG+TGG - chr1_3:73518776-73518795 Msa0139690:intron 45.0%
TGGTCAGCATCCACATCTAA+AGG - chr1_3:73518796-73518815 Msa0139690:intron 45.0%
AGAGAACCATAATGACCGAG+TGG - chr1_3:73518908-73518927 Msa0139690:intron 45.0%
!! GCATGCAGAAGTATCTCTTC+CGG + chr1_3:73518954-73518973 None:intergenic 45.0%
AGATACTTCTGCATGCAACC+AGG - chr1_3:73518958-73518977 Msa0139690:intron 45.0%
! GGATCGACTTAGCACTACTA+CGG + chr1_3:73519105-73519124 None:intergenic 45.0%
! GCTAAGTCGATCCTTTTCTC+TGG - chr1_3:73519112-73519131 Msa0139690:intron 45.0%
AGATGGCATTGCGAGGAGTT+TGG - chr1_3:73516613-73516632 Msa0139690:three_prime_UTR 50.0%
GTTAGCTATAGCGATTGGGG+TGG - chr1_3:73516654-73516673 Msa0139690:three_prime_UTR 50.0%
!! GTGGAAGTAGCAGAGGCATT+AGG - chr1_3:73516673-73516692 Msa0139690:three_prime_UTR 50.0%
!! GAGAGGGGCGTTGAAAGAAT+TGG - chr1_3:73517536-73517555 Msa0139690:intron 50.0%
ACCTGAAGTCACAGCCAATC+AGG + chr1_3:73517692-73517711 None:intergenic 50.0%
AACCACAACGCTCTTTCTGC+TGG - chr1_3:73517779-73517798 Msa0139690:intron 50.0%
!! AACCAGCAGAAAGAGCGTTG+TGG + chr1_3:73517784-73517803 None:intergenic 50.0%
GGCCCTAAACCTTATTAGGC+TGG + chr1_3:73517943-73517962 None:intergenic 50.0%
! GGGTCCATTCAGGTTTCAAG+TGG + chr1_3:73518261-73518280 None:intergenic 50.0%
ACATGAAGTCACGGCCAATC+AGG + chr1_3:73518487-73518506 None:intergenic 50.0%
GTCGCATTTGCCAGCGTTTA+AGG - chr1_3:73518733-73518752 Msa0139690:intron 50.0%
GCATTTGCCAGCGTTTAAGG+AGG - chr1_3:73518736-73518755 Msa0139690:intron 50.0%
CAGTGTCCACCTCTAACTGA+GGG + chr1_3:73518766-73518785 None:intergenic 50.0%
TCAGTGTCCACCTCTAACTG+AGG + chr1_3:73518767-73518786 None:intergenic 50.0%
CACACACCACTCGGTCATTA+TGG + chr1_3:73518917-73518936 None:intergenic 50.0%
TTCTGCATGCAACCAGGCTA+AGG - chr1_3:73518964-73518983 Msa0139690:intron 50.0%
AACCAGGCTAAGGAATGCAC+TGG - chr1_3:73518974-73518993 Msa0139690:intron 50.0%
ACCAGGCTAAGGAATGCACT+GGG - chr1_3:73518975-73518994 Msa0139690:intron 50.0%
TCCATGTACCTTGCACGCTA+GGG + chr1_3:73519043-73519062 None:intergenic 50.0%
! TGTTGTTTGGCGCTATTGCC+AGG - chr1_3:73519178-73519197 Msa0139690:CDS 50.0%
GATTGGGGTGGAAGTAGCAG+AGG - chr1_3:73516666-73516685 Msa0139690:three_prime_UTR 55.0%
! GCACCGGCACTTCTGATCAA+AGG + chr1_3:73517330-73517349 None:intergenic 55.0%
! GCCTGATTGGCTGTGACTTC+AGG - chr1_3:73517688-73517707 Msa0139690:intron 55.0%
GTCACGGCCAATCAGGCAAA+AGG + chr1_3:73518480-73518499 None:intergenic 55.0%
TCCCAGTGCATTCCTTAGCC+TGG + chr1_3:73518979-73518998 None:intergenic 55.0%
GGCTAAGGAATGCACTGGGA+AGG - chr1_3:73518979-73518998 Msa0139690:intron 55.0%
ACCAAACACCCTAGCGTGCA+AGG - chr1_3:73519032-73519051 Msa0139690:intron 55.0%
! ACCTTGCACGCTAGGGTGTT+TGG + chr1_3:73519036-73519055 None:intergenic 55.0%
ACCCTAGCGTGCAAGGTACA+TGG - chr1_3:73519039-73519058 Msa0139690:intron 55.0%
GTCCATGTACCTTGCACGCT+AGG + chr1_3:73519044-73519063 None:intergenic 55.0%
GTTGGACGGCTGACGGTGAT+CGG + chr1_3:73516579-73516598 None:intergenic 60.0%
GGTGGTTGTTGGACGGCTGA+CGG + chr1_3:73516586-73516605 None:intergenic 60.0%
GGGTTCGCCTCCTTAAACGC+TGG + chr1_3:73518746-73518765 None:intergenic 60.0%
GGAGGCGAACCCTCAGTTAG+AGG - chr1_3:73518754-73518773 Msa0139690:intron 60.0%
GGCGAACCCTCAGTTAGAGG+TGG - chr1_3:73518757-73518776 Msa0139690:intron 60.0%
TGAGCTGTGCGGCGGCGTTA+GGG + chr1_3:73516542-73516561 None:intergenic 65.0%
AGGCTCTGTGAGCTGTGCGG+CGG + chr1_3:73516550-73516569 None:intergenic 65.0%
CGGAGGCTCTGTGAGCTGTG+CGG + chr1_3:73516553-73516572 None:intergenic 65.0%
CTGACGGTGATCGGAGACGG+AGG + chr1_3:73516570-73516589 None:intergenic 65.0%
CGGCTGACGGTGATCGGAGA+CGG + chr1_3:73516573-73516592 None:intergenic 65.0%
GTGAGCTGTGCGGCGGCGTT+AGG + chr1_3:73516543-73516562 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 73516352 73519476 73516352 ID=Msa0139690;Name=Msa0139690
chr1_3 mRNA 73516352 73519476 73516352 ID=Msa0139690-mRNA-1;Parent=Msa0139690;Name=Msa0139690-mRNA-1;_AED=0.02;_eAED=0.02;_QI=263|1|1|1|1|1|3|394|119
chr1_3 exon 73516352 73516918 73516352 ID=Msa0139690-mRNA-1:exon:25622;Parent=Msa0139690-mRNA-1
chr1_3 exon 73517001 73517107 73517001 ID=Msa0139690-mRNA-1:exon:25621;Parent=Msa0139690-mRNA-1
chr1_3 exon 73519134 73519476 73519134 ID=Msa0139690-mRNA-1:exon:25620;Parent=Msa0139690-mRNA-1
chr1_3 five_prime_UTR 73519214 73519476 73519214 ID=Msa0139690-mRNA-1:five_prime_utr;Parent=Msa0139690-mRNA-1
chr1_3 CDS 73519134 73519213 73519134 ID=Msa0139690-mRNA-1:cds;Parent=Msa0139690-mRNA-1
chr1_3 CDS 73517001 73517107 73517001 ID=Msa0139690-mRNA-1:cds;Parent=Msa0139690-mRNA-1
chr1_3 CDS 73516746 73516918 73516746 ID=Msa0139690-mRNA-1:cds;Parent=Msa0139690-mRNA-1
chr1_3 three_prime_UTR 73516352 73516745 73516352 ID=Msa0139690-mRNA-1:three_prime_utr;Parent=Msa0139690-mRNA-1
Gene Sequence

>Msa0139690

ATGGCATTGCGAGGAGTTTGGCAACTGAAGAAGCTCGTTGTTAGCTATAGCGATTGGGGTGGAAGTAGCAGAGGCATTAGGGCATTTATGGAGTCGCATTTGCCAGCGTTTAAGGAGGCGAACCCTCAGTTAGAGGTGGACACTGAAATGATTCGTGGTCAGCATCCACATCTAAAGGCTTTTTACAAGAACCATAATGACCGAGTGGTGTGTGTGAAGAATATGGATCCGGAAGAGATACTTCTGCATGCAACCAGGCTAAGGAATGCACTGGGAAGGAAAGTAATTAAGCTGAGGACAAGACATGTAACCAAACACCCTAGCGTGCAAGGTACATGGACAACTGCTTTGAAATATTAG
Protein sequence

>Msa0139690

MALRGVWQLKKLVVSYSDWGGSSRGIRAFMESHLPAFKEANPQLEVDTEMIRGQHPHLKAFYKNHNDRVVCVKNMDPEEILLHATRLRNALGRKVIKLRTRHVTKHPSVQGTWTTALKY*