AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307540 MtrunA17_Chr8g0344921 99.160 119 1 0 1 119 1 119 2.01e-87 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307540 AT3G59650.1 83.193 119 20 0 1 119 1 119 7.54e-74 215
Msa1307540 AT3G59650.2 67.808 146 20 1 1 119 1 146 5.08e-68 201

Find 68 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTGATAGGGCATTTA+TGG 0.120191 8_4:-3322416 Msa1307540:intron
GTCGCATTTGCCAGCGTTTA+AGG 0.191698 8_4:-3322392 Msa1307540:CDS
CACACACCACTCGGTCATTA+TGG 0.253183 8_4:+3322211 None:intergenic
GTGAGCTGTGCGGCGGCGTT+AGG 0.286266 8_4:+3324585 None:intergenic
GTAACTATGAATTATTTCTT+TGG 0.303962 8_4:+3321722 None:intergenic
GACAACTGCTTTGAAATATT+AGG 0.339185 8_4:-3322064 Msa1307540:exon
AGATGGCATTGCGAGGAGTT+TGG 0.367673 8_4:-3324512 Msa1307540:CDS
CGTTGTTAGCTATAGCGATT+GGG 0.368754 8_4:-3324475 Msa1307540:CDS
TCCCAGTGCATTCCTTAGCC+TGG 0.373420 8_4:+3322149 None:intergenic
AATTCATAGTTACTATATAA+TGG 0.374235 8_4:-3321712 Msa1307540:three_prime_UTR
AGTGGTGTGTGTGAAGAATA+TGG 0.393785 8_4:-3322199 Msa1307540:CDS
AAAAGGTAGAAACTATATAA+TGG 0.394205 8_4:-3321671 Msa1307540:three_prime_UTR
ACCTTGCACGCTAGGGTGTT+TGG 0.399445 8_4:+3322092 None:intergenic
AAATTCTCAATCGGAGAAAA+CGG 0.414689 8_4:+3324638 None:intergenic
TGAGCTGTGCGGCGGCGTTA+GGG 0.416592 8_4:+3324586 None:intergenic
TGTTGTTTGGCGCTATTGCC+AGG 0.418369 8_4:-3321947 Msa1307540:three_prime_UTR
GCTTAAGTTCTCATGTTGTT+TGG 0.421272 8_4:-3321960 Msa1307540:three_prime_UTR
TTAATCAAATATCTGTGAAC+TGG 0.439984 8_4:-3321986 Msa1307540:three_prime_UTR
GTCCATGTACCTTGCACGCT+AGG 0.449503 8_4:+3322084 None:intergenic
GTGGAAGTAGCAGAGGCATT+AGG 0.465860 8_4:-3324452 Msa1307540:intron
GCATGCAGAAGTATCTCTTC+CGG 0.466200 8_4:+3322174 None:intergenic
GGAGGCGAACCCTCAGTTAG+AGG 0.466422 8_4:-3322371 Msa1307540:CDS
GTATGTATTAGTCAACAAAA+AGG 0.475742 8_4:-3321688 Msa1307540:three_prime_UTR
AATTCTCAATCGGAGAAAAC+GGG 0.485540 8_4:+3324639 None:intergenic
TGATTAACTGACCAGAGAAA+AGG 0.500162 8_4:+3322002 None:intergenic
ATCTGATGAATGGTGGTTGT+TGG 0.508884 8_4:+3324531 None:intergenic
ACCCTAGCGTGCAAGGTACA+TGG 0.511271 8_4:-3322086 Msa1307540:CDS
TCGTTGTTAGCTATAGCGAT+TGG 0.511918 8_4:-3324476 Msa1307540:CDS
TGGTCAGCATCCACATCTAA+AGG 0.514097 8_4:-3322329 Msa1307540:CDS
TTCTGCATGCAACCAGGCTA+AGG 0.518615 8_4:-3322161 Msa1307540:CDS
AACCAGGCTAAGGAATGCAC+TGG 0.528014 8_4:-3322151 Msa1307540:CDS
TCGCAATGCCATCTGATGAA+TGG 0.539991 8_4:+3324521 None:intergenic
TACAAACTAACAATATATAA+CGG 0.542968 8_4:+3321835 None:intergenic
ATGGTGTTTAACTGAAATCC+TGG 0.546587 8_4:+3321929 None:intergenic
GTTGGACGGCTGACGGTGAT+CGG 0.546998 8_4:+3324549 None:intergenic
GGGTTCGCCTCCTTAAACGC+TGG 0.551134 8_4:+3322382 None:intergenic
TCCATGTACCTTGCACGCTA+GGG 0.551964 8_4:+3322085 None:intergenic
GATTAACTATCAGCTGATTG+TGG 0.553497 8_4:-3321881 Msa1307540:three_prime_UTR
CGGCTGACGGTGATCGGAGA+CGG 0.561368 8_4:+3324555 None:intergenic
ACCAAACACCCTAGCGTGCA+AGG 0.562815 8_4:-3322093 Msa1307540:CDS
TCAGTGTCCACCTCTAACTG+AGG 0.586148 8_4:+3322361 None:intergenic
ACACTTAAAAGACTAAGCTA+TGG 0.587092 8_4:+3321910 None:intergenic
GTGTGTGAAGAATATGGATC+CGG 0.590195 8_4:-3322193 Msa1307540:CDS
GGCTAAGGAATGCACTGGGA+AGG 0.590269 8_4:-3322146 Msa1307540:CDS
AACAACCACCATTCATCAGA+TGG 0.591625 8_4:-3324529 Msa1307540:exon
GTGGACACTGAAATGATTCG+TGG 0.592382 8_4:-3322349 Msa1307540:CDS
GATGAATGGTGGTTGTTGGA+CGG 0.594620 8_4:+3324535 None:intergenic
AGATACTTCTGCATGCAACC+AGG 0.597112 8_4:-3322167 Msa1307540:CDS
CAGTGTCCACCTCTAACTGA+GGG 0.607705 8_4:+3322362 None:intergenic
GGATCGACTTAGCACTACTA+CGG 0.608740 8_4:+3322023 None:intergenic
TATTCTTCACACACACCACT+CGG 0.614073 8_4:+3322202 None:intergenic
GGCGAACCCTCAGTTAGAGG+TGG 0.624239 8_4:-3322368 Msa1307540:CDS
GGTGGTTGTTGGACGGCTGA+CGG 0.631122 8_4:+3324542 None:intergenic
TTTCTTTGGCGAGTAAACCA+TGG 0.636878 8_4:+3321736 None:intergenic
TTATCTTCGCTAGAGAACCA+TGG 0.637249 8_4:-3321753 Msa1307540:three_prime_UTR
GTTGTTAGCTATAGCGATTG+GGG 0.643269 8_4:-3324474 Msa1307540:CDS
GCATTTGCCAGCGTTTAAGG+AGG 0.645640 8_4:-3322389 Msa1307540:CDS
GAAGGAAAGTAATTAAGCTG+AGG 0.646557 8_4:-3322128 Msa1307540:CDS
ACCAGGCTAAGGAATGCACT+GGG 0.650150 8_4:-3322150 Msa1307540:CDS
AGGCTCTGTGAGCTGTGCGG+CGG 0.653718 8_4:+3324578 None:intergenic
ATTCATCAGATGGCATTGCG+AGG 0.654895 8_4:-3324519 Msa1307540:exon
GATTGGGGTGGAAGTAGCAG+AGG 0.657242 8_4:-3324459 Msa1307540:CDS
GTTAGCTATAGCGATTGGGG+TGG 0.672048 8_4:-3324471 Msa1307540:CDS
TATAACGGAGTACACAAACT+CGG 0.675147 8_4:+3321850 None:intergenic
CAATGCCATCTGATGAATGG+TGG 0.680972 8_4:+3324524 None:intergenic
CTGACGGTGATCGGAGACGG+AGG 0.714407 8_4:+3324558 None:intergenic
CGGAGGCTCTGTGAGCTGTG+CGG 0.730038 8_4:+3324575 None:intergenic
AGAGAACCATAATGACCGAG+TGG 0.735330 8_4:-3322217 Msa1307540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAATTAAAAGAGT+TGG - chr8_4:3321771-3321790 Msa1307540:three_prime_UTR 10.0%
!!! TATTTTTAATTAAAAGAGTT+GGG - chr8_4:3321772-3321791 Msa1307540:three_prime_UTR 10.0%
!! ATAAAAAAATGAAAACTTTA+GGG + chr8_4:3322089-3322108 None:intergenic 10.0%
!! AATAAAAAAATGAAAACTTT+AGG + chr8_4:3322090-3322109 None:intergenic 10.0%
!!! AAAGTTTTCATTTTTTTATT+TGG - chr8_4:3322090-3322109 Msa1307540:CDS 10.0%
!!! AAGTTTTCATTTTTTTATTT+GGG - chr8_4:3322091-3322110 Msa1307540:CDS 10.0%
!!! AATTTTATGAATTATGATAT+TGG - chr8_4:3322690-3322709 Msa1307540:intron 10.0%
!!! TTTTTAAAAAACTGTTTTTA+TGG - chr8_4:3322932-3322951 Msa1307540:intron 10.0%
!!! TTTTTTTAAAACTGTTTTTA+TGG - chr8_4:3323727-3323746 Msa1307540:intron 10.0%
!!! TTTAGTTTTAAATATTTGAT+AGG - chr8_4:3324018-3324037 Msa1307540:intron 10.0%
!!! TTAGTTTTAAATATTTGATA+GGG - chr8_4:3323216-3323235 Msa1307540:intron 10.0%
!!! ATTTTTTTATTTGGGTATTT+AGG - chr8_4:3322099-3322118 Msa1307540:CDS 15.0%
!! AAATCAAACAGATATAATAT+TGG - chr8_4:3322616-3322635 Msa1307540:intron 15.0%
!!! TTTTTTAAAAATTTCTGCAT+CGG + chr8_4:3322923-3322942 None:intergenic 15.0%
!!! CTTAGTTTTAAATATTTGAT+AGG - chr8_4:3323215-3323234 Msa1307540:intron 15.0%
!!! AAATGTTTAGAATGTTATTT+TGG + chr8_4:3323535-3323554 None:intergenic 15.0%
!! TACAAACTAACAATATATAA+CGG + chr8_4:3324612-3324631 None:intergenic 15.0%
!!! GATAAAAAAAAAAAGTACAA+AGG + chr8_4:3324676-3324695 None:intergenic 15.0%
!! AATTCATAGTTACTATATAA+TGG - chr8_4:3324732-3324751 Msa1307540:five_prime_UTR 15.0%
!! GAAAAATGAAAGAATGTAAA+GGG + chr8_4:3322033-3322052 None:intergenic 20.0%
!! TGAAAAATGAAAGAATGTAA+AGG + chr8_4:3322034-3322053 None:intergenic 20.0%
!!! GAAATCATTTAAAATCTGAA+TGG - chr8_4:3322808-3322827 Msa1307540:intron 20.0%
!!! GTAACTATGAATTATTTCTT+TGG + chr8_4:3324725-3324744 None:intergenic 20.0%
!!! GATTTTGAGAAATTCTCAAT+CGG + chr8_4:3321818-3321837 None:intergenic 25.0%
!!! TTAGGTTTGTTGAATTATTG+TGG - chr8_4:3322117-3322136 Msa1307540:CDS 25.0%
!!! TCTTGCTTTTATTCAAATAG+TGG - chr8_4:3322156-3322175 Msa1307540:CDS 25.0%
!! CAAATTGCTATTGTATTGTT+CGG + chr8_4:3322330-3322349 None:intergenic 25.0%
!!! TTTTTGTTTTGCTAGTTACA+AGG - chr8_4:3322433-3322452 Msa1307540:intron 25.0%
! GTTTCTAATTCTAAATGTCT+AGG - chr8_4:3322489-3322508 Msa1307540:intron 25.0%
! TTAAAAGGTCTGAAAAGAAT+AGG + chr8_4:3322564-3322583 None:intergenic 25.0%
!!! TGATATTGGTGATATTTTCT+TGG - chr8_4:3322704-3322723 Msa1307540:intron 25.0%
!!! AAAATAGTTTCTTTTTTCCC+TGG - chr8_4:3322779-3322798 Msa1307540:intron 25.0%
! TAAATGATTTCAAAACTCCA+GGG + chr8_4:3322799-3322818 None:intergenic 25.0%
! TTAAATGATTTCAAAACTCC+AGG + chr8_4:3322800-3322819 None:intergenic 25.0%
!!! AAAACTGTTTTTATGGTTTG+AGG - chr8_4:3322939-3322958 Msa1307540:intron 25.0%
! TTTGAGGACAATACTAATAA+TGG - chr8_4:3322955-3322974 Msa1307540:intron 25.0%
! ACTAATAATGGCATTGTAAA+TGG - chr8_4:3322967-3322986 Msa1307540:intron 25.0%
! AATATTTGATAGGCCATTTA+TGG - chr8_4:3323225-3323244 Msa1307540:intron 25.0%
!!! AAAATTGTTGCTTTTTTCCT+TGG - chr8_4:3323466-3323485 Msa1307540:intron 25.0%
! AAAATAGTATGCATCAGATT+CGG + chr8_4:3323704-3323723 None:intergenic 25.0%
! ATTGTTAAAAGGTTGTGATT+TGG + chr8_4:3323834-3323853 None:intergenic 25.0%
! TCTCGAATTTGATTGTTAAA+AGG + chr8_4:3323845-3323864 None:intergenic 25.0%
!! TGAATTTTACCTAGTACTAA+AGG - chr8_4:3323921-3323940 Msa1307540:intron 25.0%
!! AATATTTGATAGGGCATTTA+TGG - chr8_4:3324028-3324047 Msa1307540:intron 25.0%
!! CTTTTTACAGTAAGATTCTT+GGG - chr8_4:3324138-3324157 Msa1307540:intron 25.0%
! TTAATCAAATATCTGTGAAC+TGG - chr8_4:3324458-3324477 Msa1307540:CDS 25.0%
! GTATGTATTAGTCAACAAAA+AGG - chr8_4:3324756-3324775 Msa1307540:five_prime_UTR 25.0%
AAATTCTCAATCGGAGAAAA+CGG + chr8_4:3321809-3321828 None:intergenic 30.0%
GTTCGGTAATACACTATATT+CGG + chr8_4:3322313-3322332 None:intergenic 30.0%
TCTAGGTGATATGATAGTAT+AGG - chr8_4:3322506-3322525 Msa1307540:intron 30.0%
AAAAATTTCTGCATCGGATT+CGG + chr8_4:3322917-3322936 None:intergenic 30.0%
TCTCAGACTTGATTGTTAAA+AGG + chr8_4:3323049-3323068 None:intergenic 30.0%
! TGCATTTTACCTAGTACTAA+AGG - chr8_4:3323125-3323144 Msa1307540:intron 30.0%
TATTCATAGCATATTGTTCC+AGG + chr8_4:3323151-3323170 None:intergenic 30.0%
!! TATTCTCTCCTGTTTTGAAT+AGG - chr8_4:3323181-3323200 Msa1307540:intron 30.0%
AACAATCTCCTATTCAAAAC+AGG + chr8_4:3323192-3323211 None:intergenic 30.0%
!! TTTTTCAATGATTGACTCCT+AGG + chr8_4:3323283-3323302 None:intergenic 30.0%
! CTAGCAAGTATTAGTGATTT+TGG - chr8_4:3323304-3323323 Msa1307540:intron 30.0%
!!! TATTAGTGATTTTGGAGTCA+TGG - chr8_4:3323312-3323331 Msa1307540:intron 30.0%
!!! TGTTATTTTGGTTCTAGCAA+TGG + chr8_4:3323523-3323542 None:intergenic 30.0%
!! TCAGAGGCATTGAAAAAATA+TGG - chr8_4:3323621-3323640 Msa1307540:intron 30.0%
! TATTTTTCCTTTTGCCTGAT+TGG - chr8_4:3323789-3323808 Msa1307540:intron 30.0%
TAACAATCAAATTCGAGAGA+AGG - chr8_4:3323847-3323866 Msa1307540:intron 30.0%
AAGAATTCCCTATTCAAAAC+AGG + chr8_4:3323995-3324014 None:intergenic 30.0%
! GCTTTTTACAGTAAGATTCT+TGG - chr8_4:3324137-3324156 Msa1307540:intron 30.0%
! TGTAATTGATGATTCTCTTG+TGG - chr8_4:3324199-3324218 Msa1307540:intron 30.0%
!! GACAACTGCTTTGAAATATT+AGG - chr8_4:3324380-3324399 Msa1307540:intron 30.0%
ACACTTAAAAGACTAAGCTA+TGG + chr8_4:3324537-3324556 None:intergenic 30.0%
AATTCTCAATCGGAGAAAAC+GGG + chr8_4:3321808-3321827 None:intergenic 35.0%
ATCTCAATCATTATGTGCGT+GGG + chr8_4:3322194-3322213 None:intergenic 35.0%
AATCTCAATCATTATGTGCG+TGG + chr8_4:3322195-3322214 None:intergenic 35.0%
TTGATCAGTGTTAACGATAG+TGG - chr8_4:3322213-3322232 Msa1307540:CDS 35.0%
GCAATGCTATAGCTGTAAAT+TGG - chr8_4:3322275-3322294 Msa1307540:intron 35.0%
CAAATTTCAACTCGATTGCT+AGG - chr8_4:3322729-3322748 Msa1307540:intron 35.0%
TATTCTTCTTGTTGCCTGAT+TGG - chr8_4:3322994-3323013 Msa1307540:intron 35.0%
TAACAATCAAGTCTGAGAGA+AGG - chr8_4:3323051-3323070 Msa1307540:intron 35.0%
CTAAGAAGTCAGTAAAACCA+AGG + chr8_4:3323486-3323505 None:intergenic 35.0%
AGTCAAAGCAACTGAAAGAT+GGG + chr8_4:3323600-3323619 None:intergenic 35.0%
TAGTCAAAGCAACTGAAAGA+TGG + chr8_4:3323601-3323620 None:intergenic 35.0%
GCTAAGAATAACGTTGTAGA+TGG - chr8_4:3323762-3323781 Msa1307540:intron 35.0%
TCAGGCAAAAGGAAAAATAC+AGG + chr8_4:3323788-3323807 None:intergenic 35.0%
TTTACCTAGTACTAAAGGCT+CGG - chr8_4:3323926-3323945 Msa1307540:intron 35.0%
!! TATTCTCGCCTGTTTTGAAT+AGG - chr8_4:3323984-3324003 Msa1307540:intron 35.0%
!! ATTCTCGCCTGTTTTGAATA+GGG - chr8_4:3323985-3324004 Msa1307540:intron 35.0%
CTGTAAAAAGCCTTTAGATG+TGG + chr8_4:3324128-3324147 None:intergenic 35.0%
GAAGGAAAGTAATTAAGCTG+AGG - chr8_4:3324316-3324335 Msa1307540:intron 35.0%
TGATTAACTGACCAGAGAAA+AGG + chr8_4:3324445-3324464 None:intergenic 35.0%
GCTTAAGTTCTCATGTTGTT+TGG - chr8_4:3324484-3324503 Msa1307540:CDS 35.0%
ATGGTGTTTAACTGAAATCC+TGG + chr8_4:3324518-3324537 None:intergenic 35.0%
GATTAACTATCAGCTGATTG+TGG - chr8_4:3324563-3324582 Msa1307540:five_prime_UTR 35.0%
! TATAACGGAGTACACAAACT+CGG + chr8_4:3324597-3324616 None:intergenic 35.0%
ATCTGATGAATGGTGGTTGT+TGG + chr8_4:3321916-3321935 None:intergenic 40.0%
AACAACCACCATTCATCAGA+TGG - chr8_4:3321915-3321934 Msa1307540:three_prime_UTR 40.0%
TCGTTGTTAGCTATAGCGAT+TGG - chr8_4:3321968-3321987 Msa1307540:three_prime_UTR 40.0%
CGTTGTTAGCTATAGCGATT+GGG - chr8_4:3321969-3321988 Msa1307540:three_prime_UTR 40.0%
GTTGTTAGCTATAGCGATTG+GGG - chr8_4:3321970-3321989 Msa1307540:three_prime_UTR 40.0%
AATCATTATGTGCGTGGGTT+GGG + chr8_4:3322189-3322208 None:intergenic 40.0%
!! TCAGTGTTAACGATAGTGGA+TGG - chr8_4:3322217-3322236 Msa1307540:CDS 40.0%
! GCGGAAAATAGCGATTTGTT+TGG - chr8_4:3322239-3322258 Msa1307540:intron 40.0%
! TGTCAACGTCGGTGTTTAAA+AGG + chr8_4:3322579-3322598 None:intergenic 40.0%
ATTACTACCAGTGTCAACGT+CGG + chr8_4:3322590-3322609 None:intergenic 40.0%
! ATACCTTTGATCAGAAGTGC+CGG - chr8_4:3322643-3322662 Msa1307540:intron 40.0%
AATCAGAAGCTATGTAGCAC+CGG + chr8_4:3322665-3322684 None:intergenic 40.0%
AGTCAAAGCAACTGAAAGAC+GGG + chr8_4:3322835-3322854 None:intergenic 40.0%
CAGTCAAAGCAACTGAAAGA+CGG + chr8_4:3322836-3322855 None:intergenic 40.0%
! TTTCAGTTGCTTTGACTGAG+AGG - chr8_4:3322838-3322857 Msa1307540:intron 40.0%
! TTCAGTTGCTTTGACTGAGA+GGG - chr8_4:3322839-3322858 Msa1307540:intron 40.0%
TTTACCTAGTACTAAAGGCC+TGG - chr8_4:3323130-3323149 Msa1307540:intron 40.0%
TTGCCAGCCTAATAAGGTTT+AGG - chr8_4:3323256-3323275 Msa1307540:intron 40.0%
TGCCAGCCTAATAAGGTTTA+GGG - chr8_4:3323257-3323276 Msa1307540:intron 40.0%
! TCAGTTGCTTTGACTATCAG+AGG - chr8_4:3323605-3323624 Msa1307540:intron 40.0%
TTGCCACATCAGAATTGTTC+TGG + chr8_4:3323648-3323667 None:intergenic 40.0%
AGATTCGGAGTGTGATTACA+TGG + chr8_4:3323689-3323708 None:intergenic 40.0%
TTGGAAGTGACATGAAGTCA+CGG + chr8_4:3323815-3323834 None:intergenic 40.0%
! GACAATCAGAAGTAGCTTTG+TGG + chr8_4:3323899-3323918 None:intergenic 40.0%
TATTCTTCACACACACCACT+CGG + chr8_4:3324245-3324264 None:intergenic 40.0%
AGTGGTGTGTGTGAAGAATA+TGG - chr8_4:3324245-3324264 Msa1307540:intron 40.0%
GTGTGTGAAGAATATGGATC+CGG - chr8_4:3324251-3324270 Msa1307540:intron 40.0%
TTATCTTCGCTAGAGAACCA+TGG - chr8_4:3324691-3324710 Msa1307540:five_prime_UTR 40.0%
TTTCTTTGGCGAGTAAACCA+TGG + chr8_4:3324711-3324730 None:intergenic 40.0%
GATGAATGGTGGTTGTTGGA+CGG + chr8_4:3321912-3321931 None:intergenic 45.0%
CAATGCCATCTGATGAATGG+TGG + chr8_4:3321923-3321942 None:intergenic 45.0%
TCGCAATGCCATCTGATGAA+TGG + chr8_4:3321926-3321945 None:intergenic 45.0%
ATTCATCAGATGGCATTGCG+AGG - chr8_4:3321925-3321944 Msa1307540:three_prime_UTR 45.0%
CAATCATTATGTGCGTGGGT+TGG + chr8_4:3322190-3322209 None:intergenic 45.0%
!! GTGTTAACGATAGTGGATGG+CGG - chr8_4:3322220-3322239 Msa1307540:intron 45.0%
TTAAACACCGACGTTGACAC+TGG - chr8_4:3322580-3322599 Msa1307540:intron 45.0%
! TCAGTTGCTTTGACTGAGAG+GGG - chr8_4:3322840-3322859 Msa1307540:intron 45.0%
GGTCAAGAACAATTCTGCTG+TGG - chr8_4:3322876-3322895 Msa1307540:intron 45.0%
GAACAATTCTGCTGTGGCAA+CGG - chr8_4:3322882-3322901 Msa1307540:intron 45.0%
TGTTCCAGGCCTTTAGTACT+AGG + chr8_4:3323137-3323156 None:intergenic 45.0%
GGCAAATGCGACTCCATAAA+TGG + chr8_4:3323241-3323260 None:intergenic 45.0%
TCGCATTTGCCAGCCTAATA+AGG - chr8_4:3323250-3323269 Msa1307540:intron 45.0%
CCTAGGCCCTAAACCTTATT+AGG + chr8_4:3323266-3323285 None:intergenic 45.0%
! CCTAATAAGGTTTAGGGCCT+AGG - chr8_4:3323263-3323282 Msa1307540:intron 45.0%
GAAGCCACTTGAAACCTGAA+TGG - chr8_4:3323573-3323592 Msa1307540:intron 45.0%
ACTGAAAGATGGGTCCATTC+AGG + chr8_4:3323590-3323609 None:intergenic 45.0%
GGTCCAGAACAATTCTGATG+TGG - chr8_4:3323642-3323661 Msa1307540:intron 45.0%
ACTTCCGAGCCTTTAGTACT+AGG + chr8_4:3323933-3323952 None:intergenic 45.0%
AGTACTAAAGGCTCGGAAGT+TGG - chr8_4:3323933-3323952 Msa1307540:intron 45.0%
GTGGACACTGAAATGATTCG+TGG - chr8_4:3324095-3324114 Msa1307540:intron 45.0%
TGGTCAGCATCCACATCTAA+AGG - chr8_4:3324115-3324134 Msa1307540:intron 45.0%
AGAGAACCATAATGACCGAG+TGG - chr8_4:3324227-3324246 Msa1307540:intron 45.0%
!! GCATGCAGAAGTATCTCTTC+CGG + chr8_4:3324273-3324292 None:intergenic 45.0%
AGATACTTCTGCATGCAACC+AGG - chr8_4:3324277-3324296 Msa1307540:intron 45.0%
! GGATCGACTTAGCACTACTA+CGG + chr8_4:3324424-3324443 None:intergenic 45.0%
! GCTAAGTCGATCCTTTTCTC+TGG - chr8_4:3324431-3324450 Msa1307540:intron 45.0%
AGATGGCATTGCGAGGAGTT+TGG - chr8_4:3321932-3321951 Msa1307540:three_prime_UTR 50.0%
GTTAGCTATAGCGATTGGGG+TGG - chr8_4:3321973-3321992 Msa1307540:three_prime_UTR 50.0%
!! GTGGAAGTAGCAGAGGCATT+AGG - chr8_4:3321992-3322011 Msa1307540:three_prime_UTR 50.0%
!! GAGAGGGGCGTTGAAAGAAT+TGG - chr8_4:3322855-3322874 Msa1307540:intron 50.0%
ACCTGAAGTCACAGCCAATC+AGG + chr8_4:3323011-3323030 None:intergenic 50.0%
AACCACAACGCTCTTTCTGC+TGG - chr8_4:3323098-3323117 Msa1307540:intron 50.0%
!! AACCAGCAGAAAGAGCGTTG+TGG + chr8_4:3323103-3323122 None:intergenic 50.0%
GGCCCTAAACCTTATTAGGC+TGG + chr8_4:3323262-3323281 None:intergenic 50.0%
! GGGTCCATTCAGGTTTCAAG+TGG + chr8_4:3323580-3323599 None:intergenic 50.0%
ACATGAAGTCACGGCCAATC+AGG + chr8_4:3323806-3323825 None:intergenic 50.0%
GTCGCATTTGCCAGCGTTTA+AGG - chr8_4:3324052-3324071 Msa1307540:intron 50.0%
GCATTTGCCAGCGTTTAAGG+AGG - chr8_4:3324055-3324074 Msa1307540:intron 50.0%
CAGTGTCCACCTCTAACTGA+GGG + chr8_4:3324085-3324104 None:intergenic 50.0%
TCAGTGTCCACCTCTAACTG+AGG + chr8_4:3324086-3324105 None:intergenic 50.0%
CACACACCACTCGGTCATTA+TGG + chr8_4:3324236-3324255 None:intergenic 50.0%
TTCTGCATGCAACCAGGCTA+AGG - chr8_4:3324283-3324302 Msa1307540:intron 50.0%
AACCAGGCTAAGGAATGCAC+TGG - chr8_4:3324293-3324312 Msa1307540:intron 50.0%
ACCAGGCTAAGGAATGCACT+GGG - chr8_4:3324294-3324313 Msa1307540:intron 50.0%
TCCATGTACCTTGCACGCTA+GGG + chr8_4:3324362-3324381 None:intergenic 50.0%
! TGTTGTTTGGCGCTATTGCC+AGG - chr8_4:3324497-3324516 Msa1307540:CDS 50.0%
GATTGGGGTGGAAGTAGCAG+AGG - chr8_4:3321985-3322004 Msa1307540:three_prime_UTR 55.0%
! GCACCGGCACTTCTGATCAA+AGG + chr8_4:3322649-3322668 None:intergenic 55.0%
! GCCTGATTGGCTGTGACTTC+AGG - chr8_4:3323007-3323026 Msa1307540:intron 55.0%
GTCACGGCCAATCAGGCAAA+AGG + chr8_4:3323799-3323818 None:intergenic 55.0%
TCCCAGTGCATTCCTTAGCC+TGG + chr8_4:3324298-3324317 None:intergenic 55.0%
GGCTAAGGAATGCACTGGGA+AGG - chr8_4:3324298-3324317 Msa1307540:intron 55.0%
ACCAAACACCCTAGCGTGCA+AGG - chr8_4:3324351-3324370 Msa1307540:intron 55.0%
! ACCTTGCACGCTAGGGTGTT+TGG + chr8_4:3324355-3324374 None:intergenic 55.0%
ACCCTAGCGTGCAAGGTACA+TGG - chr8_4:3324358-3324377 Msa1307540:intron 55.0%
GTCCATGTACCTTGCACGCT+AGG + chr8_4:3324363-3324382 None:intergenic 55.0%
GTTGGACGGCTGACGGTGAT+CGG + chr8_4:3321898-3321917 None:intergenic 60.0%
GGTGGTTGTTGGACGGCTGA+CGG + chr8_4:3321905-3321924 None:intergenic 60.0%
GGGTTCGCCTCCTTAAACGC+TGG + chr8_4:3324065-3324084 None:intergenic 60.0%
GGAGGCGAACCCTCAGTTAG+AGG - chr8_4:3324073-3324092 Msa1307540:intron 60.0%
GGCGAACCCTCAGTTAGAGG+TGG - chr8_4:3324076-3324095 Msa1307540:intron 60.0%
TGAGCTGTGCGGCGGCGTTA+GGG + chr8_4:3321861-3321880 None:intergenic 65.0%
AGGCTCTGTGAGCTGTGCGG+CGG + chr8_4:3321869-3321888 None:intergenic 65.0%
CGGAGGCTCTGTGAGCTGTG+CGG + chr8_4:3321872-3321891 None:intergenic 65.0%
CTGACGGTGATCGGAGACGG+AGG + chr8_4:3321889-3321908 None:intergenic 65.0%
CGGCTGACGGTGATCGGAGA+CGG + chr8_4:3321892-3321911 None:intergenic 65.0%
GTGAGCTGTGCGGCGGCGTT+AGG + chr8_4:3321862-3321881 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 3321671 3324795 3321671 ID=Msa1307540;Name=Msa1307540
chr8_4 mRNA 3321671 3324795 3321671 ID=Msa1307540-mRNA-1;Parent=Msa1307540;Name=Msa1307540-mRNA-1;_AED=0.02;_eAED=0.02;_QI=263|1|1|1|1|1|3|394|119
chr8_4 exon 3321671 3322237 3321671 ID=Msa1307540-mRNA-1:exon:986;Parent=Msa1307540-mRNA-1
chr8_4 exon 3322320 3322426 3322320 ID=Msa1307540-mRNA-1:exon:985;Parent=Msa1307540-mRNA-1
chr8_4 exon 3324453 3324795 3324453 ID=Msa1307540-mRNA-1:exon:984;Parent=Msa1307540-mRNA-1
chr8_4 five_prime_UTR 3324533 3324795 3324533 ID=Msa1307540-mRNA-1:five_prime_utr;Parent=Msa1307540-mRNA-1
chr8_4 CDS 3324453 3324532 3324453 ID=Msa1307540-mRNA-1:cds;Parent=Msa1307540-mRNA-1
chr8_4 CDS 3322320 3322426 3322320 ID=Msa1307540-mRNA-1:cds;Parent=Msa1307540-mRNA-1
chr8_4 CDS 3322065 3322237 3322065 ID=Msa1307540-mRNA-1:cds;Parent=Msa1307540-mRNA-1
chr8_4 three_prime_UTR 3321671 3322064 3321671 ID=Msa1307540-mRNA-1:three_prime_utr;Parent=Msa1307540-mRNA-1
Gene Sequence

>Msa1307540

ATGGCATTGCGAGGAGTTTGGCAACTGAAGAAGCTCGTTGTTAGCTATAGCGATTGGGGTGGAAGTAGCAGAGGCATTAGGGCATTTATGGAGTCGCATTTGCCAGCGTTTAAGGAGGCGAACCCTCAGTTAGAGGTGGACACTGAAATGATTCGTGGTCAGCATCCACATCTAAAGGCTTTTTACAAGAACCATAATGACCGAGTGGTGTGTGTGAAGAATATGGATCCGGAAGAGATACTTCTGCATGCAACCAGGCTAAGGAATGCACTGGGAAGGAAAGTAATTAAGCTGAGGACAAGACATGTAACCAAACACCCTAGCGTGCAAGGTACATGGACAACTGCTTTGAAATATTAG
Protein sequence

>Msa1307540

MALRGVWQLKKLVVSYSDWGGSSRGIRAFMESHLPAFKEANPQLEVDTEMIRGQHPHLKAFYKNHNDRVVCVKNMDPEEILLHATRLRNALGRKVIKLRTRHVTKHPSVQGTWTTALKY*