AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0709150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0709150 MtrunA17_Chr8g0344921 99.160 119 1 0 1 119 1 119 2.01e-87 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0709150 AT3G59650.1 83.193 119 20 0 1 119 1 119 7.54e-74 215
Msa0709150 AT3G59650.2 67.808 146 20 1 1 119 1 146 5.08e-68 201

Find 68 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTGATAGGGCATTTA+TGG 0.120191 5_1:-279552 Msa0709150:intron
GTCGCATTTGCCAGCGTTTA+AGG 0.191698 5_1:-279528 Msa0709150:CDS
CACACACCACTCGGTCATTA+TGG 0.253183 5_1:+279347 None:intergenic
GTGAGCTGTGCGGCGGCGTT+AGG 0.286266 5_1:+281721 None:intergenic
GTAACTATGAATTATTTCTT+TGG 0.303962 5_1:+278859 None:intergenic
GACAACTGCTTTGAAATATT+AGG 0.339185 5_1:-279200 Msa0709150:exon
AGATGGCATTGCGAGGAGTT+TGG 0.367673 5_1:-281648 Msa0709150:CDS
CGTTGTTAGCTATAGCGATT+GGG 0.368754 5_1:-281611 Msa0709150:CDS
TCCCAGTGCATTCCTTAGCC+TGG 0.373420 5_1:+279285 None:intergenic
AATTCATAGTTACTATATAA+TGG 0.374235 5_1:-278849 Msa0709150:three_prime_UTR
AGTGGTGTGTGTGAAGAATA+TGG 0.393785 5_1:-279335 Msa0709150:CDS
AAAAGGTAGAAACTATATAA+TGG 0.394205 5_1:-278808 Msa0709150:three_prime_UTR
ACCTTGCACGCTAGGGTGTT+TGG 0.399445 5_1:+279228 None:intergenic
AAATTCTCAATCGGAGAAAA+CGG 0.414689 5_1:+281774 None:intergenic
TGAGCTGTGCGGCGGCGTTA+GGG 0.416592 5_1:+281722 None:intergenic
TGTTGTTTGGCGCTATTGCC+AGG 0.418369 5_1:-279083 Msa0709150:three_prime_UTR
GCTTAAGTTCTCATGTTGTT+TGG 0.421272 5_1:-279096 Msa0709150:three_prime_UTR
TTAATCAAATATCTGTGAAC+TGG 0.439984 5_1:-279122 Msa0709150:three_prime_UTR
GTCCATGTACCTTGCACGCT+AGG 0.449503 5_1:+279220 None:intergenic
GTGGAAGTAGCAGAGGCATT+AGG 0.465860 5_1:-281588 Msa0709150:intron
GCATGCAGAAGTATCTCTTC+CGG 0.466200 5_1:+279310 None:intergenic
GGAGGCGAACCCTCAGTTAG+AGG 0.466422 5_1:-279507 Msa0709150:CDS
GTATGTATTAGTCAACAAAA+AGG 0.475742 5_1:-278825 Msa0709150:three_prime_UTR
AATTCTCAATCGGAGAAAAC+GGG 0.485540 5_1:+281775 None:intergenic
TGATTAACTGACCAGAGAAA+AGG 0.500162 5_1:+279138 None:intergenic
ATCTGATGAATGGTGGTTGT+TGG 0.508884 5_1:+281667 None:intergenic
ACCCTAGCGTGCAAGGTACA+TGG 0.511271 5_1:-279222 Msa0709150:CDS
TCGTTGTTAGCTATAGCGAT+TGG 0.511918 5_1:-281612 Msa0709150:CDS
TGGTCAGCATCCACATCTAA+AGG 0.514097 5_1:-279465 Msa0709150:CDS
TTCTGCATGCAACCAGGCTA+AGG 0.518615 5_1:-279297 Msa0709150:CDS
AACCAGGCTAAGGAATGCAC+TGG 0.528014 5_1:-279287 Msa0709150:CDS
TCGCAATGCCATCTGATGAA+TGG 0.539991 5_1:+281657 None:intergenic
TACAAACTAACAATATATAA+CGG 0.542968 5_1:+278971 None:intergenic
ATGGTGTTTAACTGAAATCC+TGG 0.546587 5_1:+279065 None:intergenic
GTTGGACGGCTGACGGTGAT+CGG 0.546998 5_1:+281685 None:intergenic
GGGTTCGCCTCCTTAAACGC+TGG 0.551134 5_1:+279518 None:intergenic
TCCATGTACCTTGCACGCTA+GGG 0.551964 5_1:+279221 None:intergenic
GATTAACTATCAGCTGATTG+TGG 0.553497 5_1:-279017 Msa0709150:three_prime_UTR
CGGCTGACGGTGATCGGAGA+CGG 0.561368 5_1:+281691 None:intergenic
ACCAAACACCCTAGCGTGCA+AGG 0.562815 5_1:-279229 Msa0709150:CDS
TCAGTGTCCACCTCTAACTG+AGG 0.586148 5_1:+279497 None:intergenic
ACACTTAAAAGACTAAGCTA+TGG 0.587092 5_1:+279046 None:intergenic
GTGTGTGAAGAATATGGATC+CGG 0.590195 5_1:-279329 Msa0709150:CDS
GGCTAAGGAATGCACTGGGA+AGG 0.590269 5_1:-279282 Msa0709150:CDS
AACAACCACCATTCATCAGA+TGG 0.591625 5_1:-281665 Msa0709150:exon
GTGGACACTGAAATGATTCG+TGG 0.592382 5_1:-279485 Msa0709150:CDS
GATGAATGGTGGTTGTTGGA+CGG 0.594620 5_1:+281671 None:intergenic
AGATACTTCTGCATGCAACC+AGG 0.597112 5_1:-279303 Msa0709150:CDS
CAGTGTCCACCTCTAACTGA+GGG 0.607705 5_1:+279498 None:intergenic
GGATCGACTTAGCACTACTA+CGG 0.608740 5_1:+279159 None:intergenic
TATTCTTCACACACACCACT+CGG 0.614073 5_1:+279338 None:intergenic
GGCGAACCCTCAGTTAGAGG+TGG 0.624239 5_1:-279504 Msa0709150:CDS
GGTGGTTGTTGGACGGCTGA+CGG 0.631122 5_1:+281678 None:intergenic
TTTCTTTGGCGAGTAAACCA+TGG 0.636878 5_1:+278873 None:intergenic
TTATCTTCGCTAGAGAACCA+TGG 0.637249 5_1:-278890 Msa0709150:three_prime_UTR
GTTGTTAGCTATAGCGATTG+GGG 0.643269 5_1:-281610 Msa0709150:CDS
GCATTTGCCAGCGTTTAAGG+AGG 0.645640 5_1:-279525 Msa0709150:CDS
GAAGGAAAGTAATTAAGCTG+AGG 0.646557 5_1:-279264 Msa0709150:CDS
ACCAGGCTAAGGAATGCACT+GGG 0.650150 5_1:-279286 Msa0709150:CDS
AGGCTCTGTGAGCTGTGCGG+CGG 0.653718 5_1:+281714 None:intergenic
ATTCATCAGATGGCATTGCG+AGG 0.654895 5_1:-281655 Msa0709150:exon
GATTGGGGTGGAAGTAGCAG+AGG 0.657242 5_1:-281595 Msa0709150:CDS
GTTAGCTATAGCGATTGGGG+TGG 0.672048 5_1:-281607 Msa0709150:CDS
TATAACGGAGTACACAAACT+CGG 0.675147 5_1:+278986 None:intergenic
CAATGCCATCTGATGAATGG+TGG 0.680972 5_1:+281660 None:intergenic
CTGACGGTGATCGGAGACGG+AGG 0.714407 5_1:+281694 None:intergenic
CGGAGGCTCTGTGAGCTGTG+CGG 0.730038 5_1:+281711 None:intergenic
AGAGAACCATAATGACCGAG+TGG 0.735330 5_1:-279353 Msa0709150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAATTAAAAGAGT+TGG - chr5_1:278908-278927 Msa0709150:three_prime_UTR 10.0%
!!! TATTTTTAATTAAAAGAGTT+GGG - chr5_1:278909-278928 Msa0709150:three_prime_UTR 10.0%
!! ATAAAAAAATGAAAACTTTA+GGG + chr5_1:279226-279245 None:intergenic 10.0%
!! AATAAAAAAATGAAAACTTT+AGG + chr5_1:279227-279246 None:intergenic 10.0%
!!! AAAGTTTTCATTTTTTTATT+TGG - chr5_1:279227-279246 Msa0709150:CDS 10.0%
!!! AAGTTTTCATTTTTTTATTT+GGG - chr5_1:279228-279247 Msa0709150:CDS 10.0%
!!! AATTTTATGAATTATGATAT+TGG - chr5_1:279827-279846 Msa0709150:intron 10.0%
!!! TTTTTAAAAAACTGTTTTTA+TGG - chr5_1:280069-280088 Msa0709150:intron 10.0%
!!! TTTTTTTAAAACTGTTTTTA+TGG - chr5_1:280864-280883 Msa0709150:intron 10.0%
!!! TTTAGTTTTAAATATTTGAT+AGG - chr5_1:281155-281174 Msa0709150:intron 10.0%
!!! TTAGTTTTAAATATTTGATA+GGG - chr5_1:280353-280372 Msa0709150:intron 10.0%
!!! ATTTTTTTATTTGGGTATTT+AGG - chr5_1:279236-279255 Msa0709150:CDS 15.0%
!! AAATCAAACAGATATAATAT+TGG - chr5_1:279753-279772 Msa0709150:intron 15.0%
!!! TTTTTTAAAAATTTCTGCAT+CGG + chr5_1:280060-280079 None:intergenic 15.0%
!!! CTTAGTTTTAAATATTTGAT+AGG - chr5_1:280352-280371 Msa0709150:intron 15.0%
!!! AAATGTTTAGAATGTTATTT+TGG + chr5_1:280672-280691 None:intergenic 15.0%
!! TACAAACTAACAATATATAA+CGG + chr5_1:281749-281768 None:intergenic 15.0%
!!! AGATAAAAAAAAAAGTACAA+AGG + chr5_1:281813-281832 None:intergenic 15.0%
!! AATTCATAGTTACTATATAA+TGG - chr5_1:281868-281887 Msa0709150:five_prime_UTR 15.0%
!! GAAAAATGAAAGAATGTAAA+GGG + chr5_1:279170-279189 None:intergenic 20.0%
!! TGAAAAATGAAAGAATGTAA+AGG + chr5_1:279171-279190 None:intergenic 20.0%
!!! GAAATCATTTAAAATCTGAA+TGG - chr5_1:279945-279964 Msa0709150:intron 20.0%
!!! GTAACTATGAATTATTTCTT+TGG + chr5_1:281861-281880 None:intergenic 20.0%
!!! GATTTTGAGAAATTCTCAAT+CGG + chr5_1:278955-278974 None:intergenic 25.0%
!!! TTAGGTTTGTTGAATTATTG+TGG - chr5_1:279254-279273 Msa0709150:CDS 25.0%
!!! TCTTGCTTTTATTCAAATAG+TGG - chr5_1:279293-279312 Msa0709150:CDS 25.0%
!! CAAATTGCTATTGTATTGTT+CGG + chr5_1:279467-279486 None:intergenic 25.0%
!!! TTTTTGTTTTGCTAGTTACA+AGG - chr5_1:279570-279589 Msa0709150:intron 25.0%
! GTTTCTAATTCTAAATGTCT+AGG - chr5_1:279626-279645 Msa0709150:intron 25.0%
! TTAAAAGGTCTGAAAAGAAT+AGG + chr5_1:279701-279720 None:intergenic 25.0%
!!! TGATATTGGTGATATTTTCT+TGG - chr5_1:279841-279860 Msa0709150:intron 25.0%
!!! AAAATAGTTTCTTTTTTCCC+TGG - chr5_1:279916-279935 Msa0709150:intron 25.0%
! TAAATGATTTCAAAACTCCA+GGG + chr5_1:279936-279955 None:intergenic 25.0%
! TTAAATGATTTCAAAACTCC+AGG + chr5_1:279937-279956 None:intergenic 25.0%
!!! AAAACTGTTTTTATGGTTTG+AGG - chr5_1:280076-280095 Msa0709150:intron 25.0%
! TTTGAGGACAATACTAATAA+TGG - chr5_1:280092-280111 Msa0709150:intron 25.0%
! ACTAATAATGGCATTGTAAA+TGG - chr5_1:280104-280123 Msa0709150:intron 25.0%
! AATATTTGATAGGCCATTTA+TGG - chr5_1:280362-280381 Msa0709150:intron 25.0%
!!! AAAATTGTTGCTTTTTTCCT+TGG - chr5_1:280603-280622 Msa0709150:intron 25.0%
! AAAATAGTATGCATCAGATT+CGG + chr5_1:280841-280860 None:intergenic 25.0%
! ATTGTTAAAAGGTTGTGATT+TGG + chr5_1:280971-280990 None:intergenic 25.0%
! TCTCGAATTTGATTGTTAAA+AGG + chr5_1:280982-281001 None:intergenic 25.0%
!! TGAATTTTACCTAGTACTAA+AGG - chr5_1:281058-281077 Msa0709150:intron 25.0%
!! AATATTTGATAGGGCATTTA+TGG - chr5_1:281165-281184 Msa0709150:intron 25.0%
!! CTTTTTACAGTAAGATTCTT+GGG - chr5_1:281275-281294 Msa0709150:intron 25.0%
! TTAATCAAATATCTGTGAAC+TGG - chr5_1:281595-281614 Msa0709150:CDS 25.0%
! GTATGTATTAGTCAACAAAA+AGG - chr5_1:281892-281911 Msa0709150:five_prime_UTR 25.0%
AAATTCTCAATCGGAGAAAA+CGG + chr5_1:278946-278965 None:intergenic 30.0%
GTTCGGTAATACACTATATT+CGG + chr5_1:279450-279469 None:intergenic 30.0%
TCTAGGTGATATGATAGTAT+AGG - chr5_1:279643-279662 Msa0709150:intron 30.0%
AAAAATTTCTGCATCGGATT+CGG + chr5_1:280054-280073 None:intergenic 30.0%
TCTCAGACTTGATTGTTAAA+AGG + chr5_1:280186-280205 None:intergenic 30.0%
! TGCATTTTACCTAGTACTAA+AGG - chr5_1:280262-280281 Msa0709150:intron 30.0%
TATTCATAGCATATTGTTCC+AGG + chr5_1:280288-280307 None:intergenic 30.0%
!! TATTCTCTCCTGTTTTGAAT+AGG - chr5_1:280318-280337 Msa0709150:intron 30.0%
AACAATCTCCTATTCAAAAC+AGG + chr5_1:280329-280348 None:intergenic 30.0%
!! TTTTTCAATGATTGACTCCT+AGG + chr5_1:280420-280439 None:intergenic 30.0%
! CTAGCAAGTATTAGTGATTT+TGG - chr5_1:280441-280460 Msa0709150:intron 30.0%
!!! TATTAGTGATTTTGGAGTCA+TGG - chr5_1:280449-280468 Msa0709150:intron 30.0%
!!! TGTTATTTTGGTTCTAGCAA+TGG + chr5_1:280660-280679 None:intergenic 30.0%
!! TCAGAGGCATTGAAAAAATA+TGG - chr5_1:280758-280777 Msa0709150:intron 30.0%
! TATTTTTCCTTTTGCCTGAT+TGG - chr5_1:280926-280945 Msa0709150:intron 30.0%
TAACAATCAAATTCGAGAGA+AGG - chr5_1:280984-281003 Msa0709150:intron 30.0%
AAGAATTCCCTATTCAAAAC+AGG + chr5_1:281132-281151 None:intergenic 30.0%
! GCTTTTTACAGTAAGATTCT+TGG - chr5_1:281274-281293 Msa0709150:intron 30.0%
! TGTAATTGATGATTCTCTTG+TGG - chr5_1:281336-281355 Msa0709150:intron 30.0%
!! GACAACTGCTTTGAAATATT+AGG - chr5_1:281517-281536 Msa0709150:intron 30.0%
ACACTTAAAAGACTAAGCTA+TGG + chr5_1:281674-281693 None:intergenic 30.0%
AATTCTCAATCGGAGAAAAC+GGG + chr5_1:278945-278964 None:intergenic 35.0%
ATCTCAATCATTATGTGCGT+GGG + chr5_1:279331-279350 None:intergenic 35.0%
AATCTCAATCATTATGTGCG+TGG + chr5_1:279332-279351 None:intergenic 35.0%
TTGATCAGTGTTAACGATAG+TGG - chr5_1:279350-279369 Msa0709150:CDS 35.0%
GCAATGCTATAGCTGTAAAT+TGG - chr5_1:279412-279431 Msa0709150:intron 35.0%
CAAATTTCAACTCGATTGCT+AGG - chr5_1:279866-279885 Msa0709150:intron 35.0%
TATTCTTCTTGTTGCCTGAT+TGG - chr5_1:280131-280150 Msa0709150:intron 35.0%
TAACAATCAAGTCTGAGAGA+AGG - chr5_1:280188-280207 Msa0709150:intron 35.0%
CTAAGAAGTCAGTAAAACCA+AGG + chr5_1:280623-280642 None:intergenic 35.0%
AGTCAAAGCAACTGAAAGAT+GGG + chr5_1:280737-280756 None:intergenic 35.0%
TAGTCAAAGCAACTGAAAGA+TGG + chr5_1:280738-280757 None:intergenic 35.0%
GCTAAGAATAACGTTGTAGA+TGG - chr5_1:280899-280918 Msa0709150:intron 35.0%
TCAGGCAAAAGGAAAAATAC+AGG + chr5_1:280925-280944 None:intergenic 35.0%
TTTACCTAGTACTAAAGGCT+CGG - chr5_1:281063-281082 Msa0709150:intron 35.0%
!! TATTCTCGCCTGTTTTGAAT+AGG - chr5_1:281121-281140 Msa0709150:intron 35.0%
!! ATTCTCGCCTGTTTTGAATA+GGG - chr5_1:281122-281141 Msa0709150:intron 35.0%
CTGTAAAAAGCCTTTAGATG+TGG + chr5_1:281265-281284 None:intergenic 35.0%
GAAGGAAAGTAATTAAGCTG+AGG - chr5_1:281453-281472 Msa0709150:intron 35.0%
TGATTAACTGACCAGAGAAA+AGG + chr5_1:281582-281601 None:intergenic 35.0%
GCTTAAGTTCTCATGTTGTT+TGG - chr5_1:281621-281640 Msa0709150:CDS 35.0%
ATGGTGTTTAACTGAAATCC+TGG + chr5_1:281655-281674 None:intergenic 35.0%
GATTAACTATCAGCTGATTG+TGG - chr5_1:281700-281719 Msa0709150:five_prime_UTR 35.0%
! TATAACGGAGTACACAAACT+CGG + chr5_1:281734-281753 None:intergenic 35.0%
ATCTGATGAATGGTGGTTGT+TGG + chr5_1:279053-279072 None:intergenic 40.0%
AACAACCACCATTCATCAGA+TGG - chr5_1:279052-279071 Msa0709150:three_prime_UTR 40.0%
TCGTTGTTAGCTATAGCGAT+TGG - chr5_1:279105-279124 Msa0709150:three_prime_UTR 40.0%
CGTTGTTAGCTATAGCGATT+GGG - chr5_1:279106-279125 Msa0709150:three_prime_UTR 40.0%
GTTGTTAGCTATAGCGATTG+GGG - chr5_1:279107-279126 Msa0709150:three_prime_UTR 40.0%
AATCATTATGTGCGTGGGTT+GGG + chr5_1:279326-279345 None:intergenic 40.0%
!! TCAGTGTTAACGATAGTGGA+TGG - chr5_1:279354-279373 Msa0709150:CDS 40.0%
! GCGGAAAATAGCGATTTGTT+TGG - chr5_1:279376-279395 Msa0709150:intron 40.0%
! TGTCAACGTCGGTGTTTAAA+AGG + chr5_1:279716-279735 None:intergenic 40.0%
ATTACTACCAGTGTCAACGT+CGG + chr5_1:279727-279746 None:intergenic 40.0%
! ATACCTTTGATCAGAAGTGC+CGG - chr5_1:279780-279799 Msa0709150:intron 40.0%
AATCAGAAGCTATGTAGCAC+CGG + chr5_1:279802-279821 None:intergenic 40.0%
AGTCAAAGCAACTGAAAGAC+GGG + chr5_1:279972-279991 None:intergenic 40.0%
CAGTCAAAGCAACTGAAAGA+CGG + chr5_1:279973-279992 None:intergenic 40.0%
! TTTCAGTTGCTTTGACTGAG+AGG - chr5_1:279975-279994 Msa0709150:intron 40.0%
! TTCAGTTGCTTTGACTGAGA+GGG - chr5_1:279976-279995 Msa0709150:intron 40.0%
TTTACCTAGTACTAAAGGCC+TGG - chr5_1:280267-280286 Msa0709150:intron 40.0%
TTGCCAGCCTAATAAGGTTT+AGG - chr5_1:280393-280412 Msa0709150:intron 40.0%
TGCCAGCCTAATAAGGTTTA+GGG - chr5_1:280394-280413 Msa0709150:intron 40.0%
! TCAGTTGCTTTGACTATCAG+AGG - chr5_1:280742-280761 Msa0709150:intron 40.0%
TTGCCACATCAGAATTGTTC+TGG + chr5_1:280785-280804 None:intergenic 40.0%
AGATTCGGAGTGTGATTACA+TGG + chr5_1:280826-280845 None:intergenic 40.0%
TTGGAAGTGACATGAAGTCA+CGG + chr5_1:280952-280971 None:intergenic 40.0%
! GACAATCAGAAGTAGCTTTG+TGG + chr5_1:281036-281055 None:intergenic 40.0%
TATTCTTCACACACACCACT+CGG + chr5_1:281382-281401 None:intergenic 40.0%
AGTGGTGTGTGTGAAGAATA+TGG - chr5_1:281382-281401 Msa0709150:intron 40.0%
GTGTGTGAAGAATATGGATC+CGG - chr5_1:281388-281407 Msa0709150:intron 40.0%
TTATCTTCGCTAGAGAACCA+TGG - chr5_1:281827-281846 Msa0709150:five_prime_UTR 40.0%
TTTCTTTGGCGAGTAAACCA+TGG + chr5_1:281847-281866 None:intergenic 40.0%
GATGAATGGTGGTTGTTGGA+CGG + chr5_1:279049-279068 None:intergenic 45.0%
CAATGCCATCTGATGAATGG+TGG + chr5_1:279060-279079 None:intergenic 45.0%
TCGCAATGCCATCTGATGAA+TGG + chr5_1:279063-279082 None:intergenic 45.0%
ATTCATCAGATGGCATTGCG+AGG - chr5_1:279062-279081 Msa0709150:three_prime_UTR 45.0%
CAATCATTATGTGCGTGGGT+TGG + chr5_1:279327-279346 None:intergenic 45.0%
!! GTGTTAACGATAGTGGATGG+CGG - chr5_1:279357-279376 Msa0709150:intron 45.0%
TTAAACACCGACGTTGACAC+TGG - chr5_1:279717-279736 Msa0709150:intron 45.0%
! TCAGTTGCTTTGACTGAGAG+GGG - chr5_1:279977-279996 Msa0709150:intron 45.0%
GGTCAAGAACAATTCTGCTG+TGG - chr5_1:280013-280032 Msa0709150:intron 45.0%
GAACAATTCTGCTGTGGCAA+CGG - chr5_1:280019-280038 Msa0709150:intron 45.0%
TGTTCCAGGCCTTTAGTACT+AGG + chr5_1:280274-280293 None:intergenic 45.0%
GGCAAATGCGACTCCATAAA+TGG + chr5_1:280378-280397 None:intergenic 45.0%
TCGCATTTGCCAGCCTAATA+AGG - chr5_1:280387-280406 Msa0709150:intron 45.0%
CCTAGGCCCTAAACCTTATT+AGG + chr5_1:280403-280422 None:intergenic 45.0%
! CCTAATAAGGTTTAGGGCCT+AGG - chr5_1:280400-280419 Msa0709150:intron 45.0%
GAAGCCACTTGAAACCTGAA+TGG - chr5_1:280710-280729 Msa0709150:intron 45.0%
ACTGAAAGATGGGTCCATTC+AGG + chr5_1:280727-280746 None:intergenic 45.0%
GGTCCAGAACAATTCTGATG+TGG - chr5_1:280779-280798 Msa0709150:intron 45.0%
ACTTCCGAGCCTTTAGTACT+AGG + chr5_1:281070-281089 None:intergenic 45.0%
AGTACTAAAGGCTCGGAAGT+TGG - chr5_1:281070-281089 Msa0709150:intron 45.0%
GTGGACACTGAAATGATTCG+TGG - chr5_1:281232-281251 Msa0709150:intron 45.0%
TGGTCAGCATCCACATCTAA+AGG - chr5_1:281252-281271 Msa0709150:intron 45.0%
AGAGAACCATAATGACCGAG+TGG - chr5_1:281364-281383 Msa0709150:intron 45.0%
!! GCATGCAGAAGTATCTCTTC+CGG + chr5_1:281410-281429 None:intergenic 45.0%
AGATACTTCTGCATGCAACC+AGG - chr5_1:281414-281433 Msa0709150:intron 45.0%
! GGATCGACTTAGCACTACTA+CGG + chr5_1:281561-281580 None:intergenic 45.0%
! GCTAAGTCGATCCTTTTCTC+TGG - chr5_1:281568-281587 Msa0709150:intron 45.0%
AGATGGCATTGCGAGGAGTT+TGG - chr5_1:279069-279088 Msa0709150:three_prime_UTR 50.0%
GTTAGCTATAGCGATTGGGG+TGG - chr5_1:279110-279129 Msa0709150:three_prime_UTR 50.0%
!! GTGGAAGTAGCAGAGGCATT+AGG - chr5_1:279129-279148 Msa0709150:three_prime_UTR 50.0%
!! GAGAGGGGCGTTGAAAGAAT+TGG - chr5_1:279992-280011 Msa0709150:intron 50.0%
ACCTGAAGTCACAGCCAATC+AGG + chr5_1:280148-280167 None:intergenic 50.0%
AACCACAACGCTCTTTCTGC+TGG - chr5_1:280235-280254 Msa0709150:intron 50.0%
!! AACCAGCAGAAAGAGCGTTG+TGG + chr5_1:280240-280259 None:intergenic 50.0%
GGCCCTAAACCTTATTAGGC+TGG + chr5_1:280399-280418 None:intergenic 50.0%
! GGGTCCATTCAGGTTTCAAG+TGG + chr5_1:280717-280736 None:intergenic 50.0%
ACATGAAGTCACGGCCAATC+AGG + chr5_1:280943-280962 None:intergenic 50.0%
GTCGCATTTGCCAGCGTTTA+AGG - chr5_1:281189-281208 Msa0709150:intron 50.0%
GCATTTGCCAGCGTTTAAGG+AGG - chr5_1:281192-281211 Msa0709150:intron 50.0%
CAGTGTCCACCTCTAACTGA+GGG + chr5_1:281222-281241 None:intergenic 50.0%
TCAGTGTCCACCTCTAACTG+AGG + chr5_1:281223-281242 None:intergenic 50.0%
CACACACCACTCGGTCATTA+TGG + chr5_1:281373-281392 None:intergenic 50.0%
TTCTGCATGCAACCAGGCTA+AGG - chr5_1:281420-281439 Msa0709150:intron 50.0%
AACCAGGCTAAGGAATGCAC+TGG - chr5_1:281430-281449 Msa0709150:intron 50.0%
ACCAGGCTAAGGAATGCACT+GGG - chr5_1:281431-281450 Msa0709150:intron 50.0%
TCCATGTACCTTGCACGCTA+GGG + chr5_1:281499-281518 None:intergenic 50.0%
! TGTTGTTTGGCGCTATTGCC+AGG - chr5_1:281634-281653 Msa0709150:CDS 50.0%
GATTGGGGTGGAAGTAGCAG+AGG - chr5_1:279122-279141 Msa0709150:three_prime_UTR 55.0%
! GCACCGGCACTTCTGATCAA+AGG + chr5_1:279786-279805 None:intergenic 55.0%
! GCCTGATTGGCTGTGACTTC+AGG - chr5_1:280144-280163 Msa0709150:intron 55.0%
GTCACGGCCAATCAGGCAAA+AGG + chr5_1:280936-280955 None:intergenic 55.0%
TCCCAGTGCATTCCTTAGCC+TGG + chr5_1:281435-281454 None:intergenic 55.0%
GGCTAAGGAATGCACTGGGA+AGG - chr5_1:281435-281454 Msa0709150:intron 55.0%
ACCAAACACCCTAGCGTGCA+AGG - chr5_1:281488-281507 Msa0709150:intron 55.0%
! ACCTTGCACGCTAGGGTGTT+TGG + chr5_1:281492-281511 None:intergenic 55.0%
ACCCTAGCGTGCAAGGTACA+TGG - chr5_1:281495-281514 Msa0709150:intron 55.0%
GTCCATGTACCTTGCACGCT+AGG + chr5_1:281500-281519 None:intergenic 55.0%
GTTGGACGGCTGACGGTGAT+CGG + chr5_1:279035-279054 None:intergenic 60.0%
GGTGGTTGTTGGACGGCTGA+CGG + chr5_1:279042-279061 None:intergenic 60.0%
GGGTTCGCCTCCTTAAACGC+TGG + chr5_1:281202-281221 None:intergenic 60.0%
GGAGGCGAACCCTCAGTTAG+AGG - chr5_1:281210-281229 Msa0709150:intron 60.0%
GGCGAACCCTCAGTTAGAGG+TGG - chr5_1:281213-281232 Msa0709150:intron 60.0%
TGAGCTGTGCGGCGGCGTTA+GGG + chr5_1:278998-279017 None:intergenic 65.0%
AGGCTCTGTGAGCTGTGCGG+CGG + chr5_1:279006-279025 None:intergenic 65.0%
CGGAGGCTCTGTGAGCTGTG+CGG + chr5_1:279009-279028 None:intergenic 65.0%
CTGACGGTGATCGGAGACGG+AGG + chr5_1:279026-279045 None:intergenic 65.0%
CGGCTGACGGTGATCGGAGA+CGG + chr5_1:279029-279048 None:intergenic 65.0%
GTGAGCTGTGCGGCGGCGTT+AGG + chr5_1:278999-279018 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 278808 281931 278808 ID=Msa0709150;Name=Msa0709150
chr5_1 mRNA 278808 281931 278808 ID=Msa0709150-mRNA-1;Parent=Msa0709150;Name=Msa0709150-mRNA-1;_AED=0.02;_eAED=0.02;_QI=263|1|1|1|1|1|3|393|119
chr5_1 exon 278808 279373 278808 ID=Msa0709150-mRNA-1:exon:62;Parent=Msa0709150-mRNA-1
chr5_1 exon 279456 279562 279456 ID=Msa0709150-mRNA-1:exon:61;Parent=Msa0709150-mRNA-1
chr5_1 exon 281589 281931 281589 ID=Msa0709150-mRNA-1:exon:60;Parent=Msa0709150-mRNA-1
chr5_1 five_prime_UTR 281669 281931 281669 ID=Msa0709150-mRNA-1:five_prime_utr;Parent=Msa0709150-mRNA-1
chr5_1 CDS 281589 281668 281589 ID=Msa0709150-mRNA-1:cds;Parent=Msa0709150-mRNA-1
chr5_1 CDS 279456 279562 279456 ID=Msa0709150-mRNA-1:cds;Parent=Msa0709150-mRNA-1
chr5_1 CDS 279201 279373 279201 ID=Msa0709150-mRNA-1:cds;Parent=Msa0709150-mRNA-1
chr5_1 three_prime_UTR 278808 279200 278808 ID=Msa0709150-mRNA-1:three_prime_utr;Parent=Msa0709150-mRNA-1
Gene Sequence

>Msa0709150

ATGGCATTGCGAGGAGTTTGGCAACTGAAGAAGCTCGTTGTTAGCTATAGCGATTGGGGTGGAAGTAGCAGAGGCATTAGGGCATTTATGGAGTCGCATTTGCCAGCGTTTAAGGAGGCGAACCCTCAGTTAGAGGTGGACACTGAAATGATTCGTGGTCAGCATCCACATCTAAAGGCTTTTTACAAGAACCATAATGACCGAGTGGTGTGTGTGAAGAATATGGATCCGGAAGAGATACTTCTGCATGCAACCAGGCTAAGGAATGCACTGGGAAGGAAAGTAATTAAGCTGAGGACAAGACATGTAACCAAACACCCTAGCGTGCAAGGTACATGGACAACTGCTTTGAAATATTAG
Protein sequence

>Msa0709150

MALRGVWQLKKLVVSYSDWGGSSRGIRAFMESHLPAFKEANPQLEVDTEMIRGQHPHLKAFYKNHNDRVVCVKNMDPEEILLHATRLRNALGRKVIKLRTRHVTKHPSVQGTWTTALKY*