AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0155740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0155740 MtrunA17_Chr1g0155861 98.261 115 2 0 1 115 1 115 2.46e-82 236
Msa0155740 MtrunA17_Chr8g0392451 97.391 115 3 0 1 115 1 115 7.81e-81 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0155740 AT3G01435.1 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0155740 AT3G01435.4 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0155740 AT3G01435.2 57.534 146 30 1 1 114 1 146 5.45e-54 166
Msa0155740 AT3G01435.3 70.238 84 25 0 31 114 1 84 6.96e-42 133

Find 55 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTCAAGACAGTGATTT+TGG 0.154549 1_4:-21315414 None:intergenic
GGTTAGGGTTCTTTGAAAAT+TGG 0.180356 1_4:+21313414 Msa0155740:five_prime_UTR
CATATCTCATTTGCTATTCT+TGG 0.183234 1_4:-21317172 None:intergenic
TGAAAATTCAGTCTGTAATT+TGG 0.197049 1_4:+21317314 Msa0155740:three_prime_UTR
TGAATTCATGCAATTAATTA+AGG 0.202211 1_4:+21315429 Msa0155740:CDS
GACTTTCTTGGAAGAATATT+GGG 0.218800 1_4:-21313291 None:intergenic
TGACTTTCTTGGAAGAATAT+TGG 0.221095 1_4:-21313292 None:intergenic
TCAAATGCATATGACTTTCT+TGG 0.235673 1_4:-21313303 None:intergenic
GTTAGGGTTCTTTGAAAATT+GGG 0.254874 1_4:+21313415 Msa0155740:five_prime_UTR
AAAGACAGATTATGAAGTTT+TGG 0.264512 1_4:+21317462 Msa0155740:three_prime_UTR
CCATTTGAAAAGTGTGATTA+TGG 0.272416 1_4:+21317148 Msa0155740:CDS
AAGCTTATTTAATTGTCTAA+TGG 0.277330 1_4:-21313344 None:intergenic
ATAGAGAATTGTGAAGGTTT+TGG 0.292105 1_4:+21315330 Msa0155740:intron
AGCTTATTTAATTGTCTAAT+GGG 0.310583 1_4:-21313343 None:intergenic
GGGAGGAAATAGAGTAAATA+AGG 0.319714 1_4:+21313460 Msa0155740:five_prime_UTR
GTGCAACTTGAATTGCTTGC+AGG 0.330825 1_4:-21317349 None:intergenic
GGAGAAAGACTATTAAGGTT+AGG 0.339063 1_4:+21313398 Msa0155740:five_prime_UTR
TCTTTGAAAATTGGGGAAAA+AGG 0.342786 1_4:+21313423 Msa0155740:five_prime_UTR
ATTTATTTCGAGGGGTCATT+TGG 0.349026 1_4:+21317399 Msa0155740:three_prime_UTR
ACCAAGGAGAAAGACTATTA+AGG 0.372416 1_4:+21313393 Msa0155740:five_prime_UTR
AAAAGGTAGAGCTAGGATTT+GGG 0.386260 1_4:+21313440 Msa0155740:five_prime_UTR
ATAAGCTTAGTGAAAGTGAT+TGG 0.391447 1_4:+21313359 Msa0155740:five_prime_UTR
AAATGAGATATGTTTCAAAA+AGG 0.396887 1_4:+21317183 Msa0155740:CDS
GATGGTGATGTTTGAGTGTT+TGG 0.396997 1_4:-21313257 None:intergenic
ATATCTCATTTGCTATTCTT+GGG 0.398050 1_4:-21317171 None:intergenic
ATTGTTAATTGTTAAGAGAA+AGG 0.407894 1_4:+21313201 None:intergenic
GAGAAAGACTATTAAGGTTA+GGG 0.417995 1_4:+21313399 Msa0155740:five_prime_UTR
ACCTTAATAGTCTTTCTCCT+TGG 0.422251 1_4:-21313394 None:intergenic
GGAATATGTCATGTCACAAT+TGG 0.423342 1_4:+21317207 Msa0155740:CDS
AGCAACATCAATGGATTCAC+AGG 0.432506 1_4:+21315163 Msa0155740:exon
TTTATTTCGAGGGGTCATTT+GGG 0.435975 1_4:+21317400 Msa0155740:three_prime_UTR
TTGTTAATTGTTAAGAGAAA+GGG 0.451821 1_4:+21313202 None:intergenic
GTCTTTGCAATGAAGTTGTC+TGG 0.456591 1_4:-21315187 None:intergenic
AACTTTCATCCTTAGTATCA+AGG 0.457524 1_4:+21313505 Msa0155740:five_prime_UTR
TTTCATCACGCAACGCCACT+TGG 0.479312 1_4:-21317102 None:intergenic
AGGAACCCACCTTGATACTA+AGG 0.497854 1_4:-21313514 None:intergenic
ATAGAGTAAATAAGGAACAT+TGG 0.498437 1_4:+21313468 Msa0155740:five_prime_UTR
TATTTATGTATTTATTTCGA+GGG 0.499343 1_4:+21317390 Msa0155740:three_prime_UTR
TTGATACTAAGGATGAAAGT+TGG 0.502389 1_4:-21313503 None:intergenic
TTATTTATGTATTTATTTCG+AGG 0.502816 1_4:+21317389 Msa0155740:three_prime_UTR
CACAGAGAAAGCAACATCAA+TGG 0.506024 1_4:+21315154 Msa0155740:exon
TTAGGGTTCTTTGAAAATTG+GGG 0.511126 1_4:+21313416 Msa0155740:five_prime_UTR
ATGTCTTTAATCTCAAACAT+AGG 0.521066 1_4:-21317533 None:intergenic
TGCAACTTGAATTGCTTGCA+GGG 0.527566 1_4:-21317348 None:intergenic
TTGTCAATTGTGAACTATCT+CGG 0.532804 1_4:+21313234 Msa0155740:five_prime_UTR
GACGAGCTTGCAAGCCCAGT+TGG 0.535324 1_4:+21315373 Msa0155740:CDS
AAGACTTCAGAATGTCGAAA+AGG 0.556491 1_4:+21315205 Msa0155740:CDS
GGAGCTTGCAGGAGGAGTGA+TGG 0.557814 1_4:+21315351 Msa0155740:CDS
ACTTTCTTGGAAGAATATTG+GGG 0.595084 1_4:-21313290 None:intergenic
AGGTAGAGCTAGGATTTGGG+AGG 0.607159 1_4:+21313443 Msa0155740:five_prime_UTR
AATTCAAGTTGCACTCAACA+AGG 0.612180 1_4:+21317358 Msa0155740:three_prime_UTR
ATTTATGTATTTATTTCGAG+GGG 0.636208 1_4:+21317391 Msa0155740:three_prime_UTR
GTTTGTATAGAGAATTGTGA+AGG 0.675616 1_4:+21315324 Msa0155740:intron
ATTGGAGAGTAAATCAACCA+AGG 0.682126 1_4:+21313377 Msa0155740:five_prime_UTR
GCAACATCAATGGATTCACA+GGG 0.701218 1_4:+21315164 Msa0155740:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAGAATTATGAAAT+TGG + chr1_4:21313563-21313582 Msa0155740:intron 10.0%
!! ATATTAAAGTATATTAAAGA+AGG + chr1_4:21314436-21314455 Msa0155740:intron 10.0%
!! TATGATATTAATTATTGAAA+TGG - chr1_4:21315533-21315552 None:intergenic 10.0%
!! ATAATTTATCAAATGAATGA+GGG - chr1_4:21313550-21313569 None:intergenic 15.0%
!! TATAATTTATCAAATGAATG+AGG - chr1_4:21313551-21313570 None:intergenic 15.0%
!! TATAGTCTATAATTATTAAG+AGG - chr1_4:21314036-21314055 None:intergenic 15.0%
!! AATAAAGAAATCAACATAAT+GGG - chr1_4:21314601-21314620 None:intergenic 15.0%
!! TAATAAAGAAATCAACATAA+TGG - chr1_4:21314602-21314621 None:intergenic 15.0%
!!! ATTATGTTGATTTCTTTATT+AGG + chr1_4:21314601-21314620 Msa0155740:intron 15.0%
!! TAAAAAAAATCAAGTATGAA+AGG - chr1_4:21315273-21315292 None:intergenic 15.0%
!! ATGAATAAGTGTTTATAAAT+GGG - chr1_4:21315831-21315850 None:intergenic 15.0%
!!! TGATTTTCAATTACAATTAA+CGG + chr1_4:21316238-21316257 Msa0155740:intron 15.0%
!! TTCATTTCATATCTTTAATT+TGG - chr1_4:21316691-21316710 None:intergenic 15.0%
!!! AAACTATTTTTGATAGAAAT+GGG - chr1_4:21316716-21316735 None:intergenic 15.0%
!!! TAAACTATTTTTGATAGAAA+TGG - chr1_4:21316717-21316736 None:intergenic 15.0%
!! AATATTACAACATTATAGTA+GGG - chr1_4:21317026-21317045 None:intergenic 15.0%
!! AAAAATTAGAAAGAAAATGA+AGG - chr1_4:21317073-21317092 None:intergenic 15.0%
!!! ATTTTCTTTCTAATTTTTGT+AGG + chr1_4:21317075-21317094 Msa0155740:intron 15.0%
!! TTATTTATGTATTTATTTCG+AGG + chr1_4:21317389-21317408 Msa0155740:three_prime_UTR 15.0%
!! TATTTATGTATTTATTTCGA+GGG + chr1_4:21317390-21317409 Msa0155740:three_prime_UTR 15.0%
!! AGCTTATTTAATTGTCTAAT+GGG - chr1_4:21313346-21313365 None:intergenic 20.0%
!! AAGCTTATTTAATTGTCTAA+TGG - chr1_4:21313347-21313366 None:intergenic 20.0%
!! AGAATTATGAAATTGGAATT+TGG + chr1_4:21313570-21313589 Msa0155740:intron 20.0%
!! GAATTATGAAATTGGAATTT+GGG + chr1_4:21313571-21313590 Msa0155740:intron 20.0%
!! TTAGTAATATTCATATCTAG+TGG - chr1_4:21314134-21314153 None:intergenic 20.0%
!! TACTTGATAAAAATCTTACA+CGG - chr1_4:21314785-21314804 None:intergenic 20.0%
!! AAAATCTCTAAACAAATTTG+TGG + chr1_4:21314835-21314854 Msa0155740:intron 20.0%
!! AAGATATCAGGAAAAAAATT+AGG + chr1_4:21315021-21315040 Msa0155740:intron 20.0%
!! TGAATTCATGCAATTAATTA+AGG + chr1_4:21315429-21315448 Msa0155740:CDS 20.0%
!!! TAGTTGTTACACAATAATTT+TGG - chr1_4:21315568-21315587 None:intergenic 20.0%
!! AATTATTGTGTAACAACTAT+TGG + chr1_4:21315569-21315588 Msa0155740:intron 20.0%
!! TGAATAAGTGTTTATAAATG+GGG - chr1_4:21315830-21315849 None:intergenic 20.0%
!! CATGAATAAGTGTTTATAAA+TGG - chr1_4:21315832-21315851 None:intergenic 20.0%
!! TTCAATATATAAACTATGCT+TGG + chr1_4:21315880-21315899 Msa0155740:intron 20.0%
!! ACTCAATTACACAAATTTAA+AGG - chr1_4:21316349-21316368 None:intergenic 20.0%
!! GAATATTACAACATTATAGT+AGG - chr1_4:21317027-21317046 None:intergenic 20.0%
!! AAATGAGATATGTTTCAAAA+AGG + chr1_4:21317183-21317202 Msa0155740:CDS 20.0%
!!! ATTTAAGAAGCAAAATTTTC+AGG - chr1_4:21317272-21317291 None:intergenic 20.0%
!! ATTTATGTATTTATTTCGAG+GGG + chr1_4:21317391-21317410 Msa0155740:three_prime_UTR 20.0%
! ATAGAGTAAATAAGGAACAT+TGG + chr1_4:21313468-21313487 Msa0155740:five_prime_UTR 25.0%
!! ATAATATGATGATGTCCAAA+TGG - chr1_4:21313895-21313914 None:intergenic 25.0%
!!! TAGTTGATGTTTTTTATCAG+TGG + chr1_4:21314054-21314073 Msa0155740:intron 25.0%
! TTTATCAGTGGAATGAATAT+TGG + chr1_4:21314066-21314085 Msa0155740:intron 25.0%
!! CTAGTAGAAGATATTGATTA+AGG + chr1_4:21314157-21314176 Msa0155740:intron 25.0%
! ATTGTTATAGCATACTACAT+TGG + chr1_4:21314334-21314353 Msa0155740:intron 25.0%
!! ATTGGCAGTACAAATTATTT+TGG + chr1_4:21314352-21314371 Msa0155740:intron 25.0%
!! ATTTCCCTTTTTGTAAAATG+TGG + chr1_4:21314700-21314719 Msa0155740:intron 25.0%
!! TTTTCTAAGCAGTAAACTAA+TGG - chr1_4:21314907-21314926 None:intergenic 25.0%
! AATCTTGCCAAAATATTGTA+GGG - chr1_4:21315069-21315088 None:intergenic 25.0%
! TAATCTTGCCAAAATATTGT+AGG - chr1_4:21315070-21315089 None:intergenic 25.0%
!! TTTTTATTACAAGATTGCAC+TGG + chr1_4:21315482-21315501 Msa0155740:intron 25.0%
! TATATAACTATAGATGCAAG+TGG + chr1_4:21315505-21315524 Msa0155740:intron 25.0%
! GAATAAGTGTTTATAAATGG+GGG - chr1_4:21315829-21315848 None:intergenic 25.0%
! TATGTTGCATACAAATGAAA+GGG - chr1_4:21315940-21315959 None:intergenic 25.0%
!!! CTATTTCACATAACTGATTT+TGG + chr1_4:21316033-21316052 Msa0155740:intron 25.0%
! GACTATTAGCACTTAAAAAA+TGG + chr1_4:21316378-21316397 Msa0155740:intron 25.0%
! TTCAAATACCAAACTTACAA+AGG - chr1_4:21316512-21316531 None:intergenic 25.0%
! TAATGAACATTAGATTTCTC+AGG - chr1_4:21316536-21316555 None:intergenic 25.0%
! CTATCAAAAATAGTTTACCT+TGG + chr1_4:21316720-21316739 Msa0155740:intron 25.0%
! AAACAACTAAGTACATGTTT+GGG + chr1_4:21316747-21316766 Msa0155740:intron 25.0%
! AACACAAATAATTCTTCTAG+AGG - chr1_4:21316827-21316846 None:intergenic 25.0%
! TATATACTCCAATAACTAAC+AGG - chr1_4:21316888-21316907 None:intergenic 25.0%
!!! ATATCTCATTTGCTATTCTT+GGG - chr1_4:21317174-21317193 None:intergenic 25.0%
! TGAAAATTCAGTCTGTAATT+TGG + chr1_4:21317314-21317333 Msa0155740:three_prime_UTR 25.0%
!! TAATCTGTCTTTTACATGTT+TGG - chr1_4:21317454-21317473 None:intergenic 25.0%
!! AAAGACAGATTATGAAGTTT+TGG + chr1_4:21317462-21317481 Msa0155740:three_prime_UTR 25.0%
TTGTCAATTGTGAACTATCT+CGG + chr1_4:21313234-21313253 Msa0155740:five_prime_UTR 30.0%
ACTTTCTTGGAAGAATATTG+GGG - chr1_4:21313293-21313312 None:intergenic 30.0%
GACTTTCTTGGAAGAATATT+GGG - chr1_4:21313294-21313313 None:intergenic 30.0%
TGACTTTCTTGGAAGAATAT+TGG - chr1_4:21313295-21313314 None:intergenic 30.0%
TCAAATGCATATGACTTTCT+TGG - chr1_4:21313306-21313325 None:intergenic 30.0%
ATAAGCTTAGTGAAAGTGAT+TGG + chr1_4:21313359-21313378 Msa0155740:five_prime_UTR 30.0%
GAGAAAGACTATTAAGGTTA+GGG + chr1_4:21313399-21313418 Msa0155740:five_prime_UTR 30.0%
! GTTAGGGTTCTTTGAAAATT+GGG + chr1_4:21313415-21313434 Msa0155740:five_prime_UTR 30.0%
! TTAGGGTTCTTTGAAAATTG+GGG + chr1_4:21313416-21313435 Msa0155740:five_prime_UTR 30.0%
! TCTTTGAAAATTGGGGAAAA+AGG + chr1_4:21313423-21313442 Msa0155740:five_prime_UTR 30.0%
TTGATACTAAGGATGAAAGT+TGG - chr1_4:21313506-21313525 None:intergenic 30.0%
AACTTTCATCCTTAGTATCA+AGG + chr1_4:21313505-21313524 Msa0155740:five_prime_UTR 30.0%
! AAAGCATTCAAATACATCGA+AGG - chr1_4:21313770-21313789 None:intergenic 30.0%
CAGATTAAGATTGCACATTA+AGG + chr1_4:21313861-21313880 Msa0155740:intron 30.0%
ATTAAGGTCATAACTCCATT+TGG + chr1_4:21313877-21313896 Msa0155740:intron 30.0%
AAGTGAATAAGAATGAAGTC+TGG + chr1_4:21313930-21313949 Msa0155740:intron 30.0%
CAACAGATTGTATCGAAAAT+AGG + chr1_4:21313987-21314006 Msa0155740:intron 30.0%
AAATAGGATAGAACATGCAA+TGG + chr1_4:21314003-21314022 Msa0155740:intron 30.0%
TAGGTAGTGTTTAGATAATG+TGG + chr1_4:21314203-21314222 Msa0155740:intron 30.0%
AGGTAGTGTTTAGATAATGT+GGG + chr1_4:21314204-21314223 Msa0155740:intron 30.0%
CAATAATAAAGCTTTGCAAG+TGG - chr1_4:21314318-21314337 None:intergenic 30.0%
! ACTAGAAATTGATTCCAACT+TGG + chr1_4:21314522-21314541 Msa0155740:intron 30.0%
ATAAACATAGAATGCCAAGT+TGG - chr1_4:21314539-21314558 None:intergenic 30.0%
ATTCTATGTTTATCCCGATT+TGG + chr1_4:21314546-21314565 Msa0155740:intron 30.0%
TTCTATGTTTATCCCGATTT+GGG + chr1_4:21314547-21314566 Msa0155740:intron 30.0%
!! TAAGCTATTTTGCTGTCAAT+CGG + chr1_4:21314626-21314645 Msa0155740:intron 30.0%
! TCAGCCACATTTTACAAAAA+GGG - chr1_4:21314707-21314726 None:intergenic 30.0%
! ATCAGCCACATTTTACAAAA+AGG - chr1_4:21314708-21314727 None:intergenic 30.0%
TTGATAAAAATCTTACACGG+CGG - chr1_4:21314782-21314801 None:intergenic 30.0%
! AACTAATGGTGTGTTTGTTT+TGG - chr1_4:21314893-21314912 None:intergenic 30.0%
GTTTGTATAGAGAATTGTGA+AGG + chr1_4:21315324-21315343 Msa0155740:intron 30.0%
! ATAGAGAATTGTGAAGGTTT+TGG + chr1_4:21315330-21315349 Msa0155740:intron 30.0%
! TTTTGGACAAGATCTTTTCT+AGG - chr1_4:21315400-21315419 None:intergenic 30.0%
! TGAATTCAAGACAGTGATTT+TGG - chr1_4:21315417-21315436 None:intergenic 30.0%
AATTATTGAGAGACAAAGCA+AGG - chr1_4:21315459-21315478 None:intergenic 30.0%
CTAACTCATCCTTACAAAAT+CGG - chr1_4:21315771-21315790 None:intergenic 30.0%
ATTGAAATGTCTACGTACAT+CGG - chr1_4:21315866-21315885 None:intergenic 30.0%
ATGGGAATCACCATAAAAAA+GGG - chr1_4:21315915-21315934 None:intergenic 30.0%
TATGGGAATCACCATAAAAA+AGG - chr1_4:21315916-21315935 None:intergenic 30.0%
CATACAAATGAAAGGGTTAT+GGG - chr1_4:21315933-21315952 None:intergenic 30.0%
GTATGTTGCATACAAATGAA+AGG - chr1_4:21315941-21315960 None:intergenic 30.0%
TGAAATAGCAAATACTGTCA+CGG - chr1_4:21316021-21316040 None:intergenic 30.0%
ATGTAATCAATAGCAGATTG+CGG - chr1_4:21316070-21316089 None:intergenic 30.0%
AACAGATAGAATTGTGATCA+AGG - chr1_4:21316219-21316238 None:intergenic 30.0%
TACCTAAAAAGAGGATAAAG+CGG - chr1_4:21316272-21316291 None:intergenic 30.0%
CAGATTTGATACCTAAAAAG+AGG - chr1_4:21316281-21316300 None:intergenic 30.0%
TATCACAACCAAACTTACAA+AGG - chr1_4:21316481-21316500 None:intergenic 30.0%
AAACAACACTAGTTATGTGA+TGG + chr1_4:21316635-21316654 Msa0155740:intron 30.0%
CAAACATGTACTTAGTTGTT+TGG - chr1_4:21316748-21316767 None:intergenic 30.0%
CAAACAACTAAGTACATGTT+TGG + chr1_4:21316746-21316765 Msa0155740:intron 30.0%
TAAGTACATGTTTGGGTAAT+TGG + chr1_4:21316754-21316773 Msa0155740:intron 30.0%
ACTAAACCTGTTAGCAATTA+GGG - chr1_4:21316956-21316975 None:intergenic 30.0%
AACTAAACCTGTTAGCAATT+AGG - chr1_4:21316957-21316976 None:intergenic 30.0%
CTAACAGGTTTAGTTATTCA+GGG + chr1_4:21316962-21316981 Msa0155740:intron 30.0%
! CCATAATCACACTTTTCAAA+TGG - chr1_4:21317151-21317170 None:intergenic 30.0%
! CCATTTGAAAAGTGTGATTA+TGG + chr1_4:21317148-21317167 Msa0155740:CDS 30.0%
!! CATATCTCATTTGCTATTCT+TGG - chr1_4:21317175-21317194 None:intergenic 30.0%
TGAGATATGTTTCAAAAAGG+TGG + chr1_4:21317186-21317205 Msa0155740:CDS 30.0%
TATTGGGGAAAAAGCTATGA+TGG - chr1_4:21313278-21313297 None:intergenic 35.0%
ATTGGAGAGTAAATCAACCA+AGG + chr1_4:21313377-21313396 Msa0155740:five_prime_UTR 35.0%
ACCAAGGAGAAAGACTATTA+AGG + chr1_4:21313393-21313412 Msa0155740:five_prime_UTR 35.0%
ACCTTAATAGTCTTTCTCCT+TGG - chr1_4:21313397-21313416 None:intergenic 35.0%
GGAGAAAGACTATTAAGGTT+AGG + chr1_4:21313398-21313417 Msa0155740:five_prime_UTR 35.0%
! GGTTAGGGTTCTTTGAAAAT+TGG + chr1_4:21313414-21313433 Msa0155740:five_prime_UTR 35.0%
AAAAAGGTAGAGCTAGGATT+TGG + chr1_4:21313439-21313458 Msa0155740:five_prime_UTR 35.0%
AAAAGGTAGAGCTAGGATTT+GGG + chr1_4:21313440-21313459 Msa0155740:five_prime_UTR 35.0%
GGGAGGAAATAGAGTAAATA+AGG + chr1_4:21313460-21313479 Msa0155740:five_prime_UTR 35.0%
TTTCATCCTTAGTATCAAGG+TGG + chr1_4:21313508-21313527 Msa0155740:intron 35.0%
TTCATCCTTAGTATCAAGGT+GGG + chr1_4:21313509-21313528 Msa0155740:intron 35.0%
ATCAAATGAATGAGGGATGA+AGG - chr1_4:21313543-21313562 None:intergenic 35.0%
ATGTGCAATCTTAATCTGCA+AGG - chr1_4:21313859-21313878 None:intergenic 35.0%
ATACAGTGTGAACCTGATAT+AGG + chr1_4:21314102-21314121 Msa0155740:intron 35.0%
TACAGTGTGAACCTGATATA+GGG + chr1_4:21314103-21314122 Msa0155740:intron 35.0%
TAGTTGCAATTTGTGCAACT+AGG + chr1_4:21314184-21314203 Msa0155740:intron 35.0%
GGTAGTGTTTAGATAATGTG+GGG + chr1_4:21314205-21314224 Msa0155740:intron 35.0%
!! TTTTGCTGTCAATCGGATAT+CGG + chr1_4:21314633-21314652 Msa0155740:intron 35.0%
! TGTCTAGAGTCTAGAATTCT+CGG + chr1_4:21314664-21314683 Msa0155740:intron 35.0%
AGTGTTGCGTAAAGATTGAT+TGG + chr1_4:21314934-21314953 Msa0155740:intron 35.0%
AAGACTTCAGAATGTCGAAA+AGG + chr1_4:21315205-21315224 Msa0155740:CDS 35.0%
ATTGGAAATTCTGCCTTCAT+TGG + chr1_4:21315587-21315606 Msa0155740:intron 35.0%
TTCATTGTGTTGGGTTTGAA+GGG + chr1_4:21315671-21315690 Msa0155740:intron 35.0%
GTCATATCTCTAAGTGATGT+GGG - chr1_4:21315708-21315727 None:intergenic 35.0%
CCAATGCTTTATAAACACAC+AGG - chr1_4:21315730-21315749 None:intergenic 35.0%
CCTGTGTGTTTATAAAGCAT+TGG + chr1_4:21315727-21315746 Msa0155740:intron 35.0%
! CGATTTTGTAAGGATGAGTT+AGG + chr1_4:21315769-21315788 Msa0155740:intron 35.0%
ATCACCATAAAAAAGGGGAA+TGG - chr1_4:21315909-21315928 None:intergenic 35.0%
TGGGAATCACCATAAAAAAG+GGG - chr1_4:21315914-21315933 None:intergenic 35.0%
GCATACAAATGAAAGGGTTA+TGG - chr1_4:21315934-21315953 None:intergenic 35.0%
GAAATAGCAAATACTGTCAC+GGG - chr1_4:21316020-21316039 None:intergenic 35.0%
! ATCCGCTTTATCCTCTTTTT+AGG + chr1_4:21316267-21316286 Msa0155740:intron 35.0%
! AAAATTGTCCTGTTTCTCCA+TGG + chr1_4:21316410-21316429 Msa0155740:intron 35.0%
AAGCATGAAAATACACACCA+TGG - chr1_4:21316430-21316449 None:intergenic 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_4:21316470-21316489 Msa0155740:intron 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_4:21316470-21316489 Msa0155740:intron 35.0%
TACTTAGTTGTTTGGATCCA+AGG - chr1_4:21316740-21316759 None:intergenic 35.0%
TAATTGGTGCAAACTACAGA+GGG + chr1_4:21316770-21316789 Msa0155740:intron 35.0%
TCTTCTGAGTGCATTGTATT+TGG + chr1_4:21316793-21316812 Msa0155740:intron 35.0%
AGTTCTGTCCTGTTAGTTAT+TGG + chr1_4:21316877-21316896 Msa0155740:intron 35.0%
GCTAACAGGTTTAGTTATTC+AGG + chr1_4:21316961-21316980 Msa0155740:intron 35.0%
! ATTTTTGTAGGACATCCAAG+TGG + chr1_4:21317087-21317106 Msa0155740:intron 35.0%
GGAATATGTCATGTCACAAT+TGG + chr1_4:21317207-21317226 Msa0155740:CDS 35.0%
!! AATTCAAGTTGCACTCAACA+AGG + chr1_4:21317358-21317377 Msa0155740:three_prime_UTR 35.0%
ATTTATTTCGAGGGGTCATT+TGG + chr1_4:21317399-21317418 Msa0155740:three_prime_UTR 35.0%
TTTATTTCGAGGGGTCATTT+GGG + chr1_4:21317400-21317419 Msa0155740:three_prime_UTR 35.0%
! GATGGTGATGTTTGAGTGTT+TGG - chr1_4:21313260-21313279 None:intergenic 40.0%
! CTGAGTTGCTTTCATGTGTA+TGG + chr1_4:21313714-21313733 Msa0155740:intron 40.0%
! AGTTGCTTTCATGTGTATGG+AGG + chr1_4:21313717-21313736 Msa0155740:intron 40.0%
CGATACAATCTGTTGCATGA+AGG - chr1_4:21313982-21314001 None:intergenic 40.0%
TAGTGGCATTACCCTATATC+AGG - chr1_4:21314117-21314136 None:intergenic 40.0%
TGGGGAAGATTAGTAAGCTA+AGG + chr1_4:21314223-21314242 Msa0155740:intron 40.0%
! CCTAAGGTTTTTCACTGTGT+CGG - chr1_4:21314256-21314275 None:intergenic 40.0%
CAGTGAAAAACCTTAGGTGT+CGG + chr1_4:21314259-21314278 Msa0155740:intron 40.0%
TACATTGTAACCGACACCTA+AGG - chr1_4:21314272-21314291 None:intergenic 40.0%
!! TTAGGTGTCGGTTACAATGT+AGG + chr1_4:21314271-21314290 Msa0155740:intron 40.0%
! AAGAAGGCATGTGATGCTAA+CGG + chr1_4:21314452-21314471 Msa0155740:intron 40.0%
TCTGCATAATCCAACTTGTG+CGG + chr1_4:21314992-21315011 Msa0155740:intron 40.0%
! TACCGCTCCCTACAATATTT+TGG + chr1_4:21315059-21315078 Msa0155740:intron 40.0%
TGCCAAAATATTGTAGGGAG+CGG - chr1_4:21315064-21315083 None:intergenic 40.0%
CACAGAGAAAGCAACATCAA+TGG + chr1_4:21315154-21315173 Msa0155740:exon 40.0%
! AGCAACATCAATGGATTCAC+AGG + chr1_4:21315163-21315182 Msa0155740:exon 40.0%
! GCAACATCAATGGATTCACA+GGG + chr1_4:21315164-21315183 Msa0155740:exon 40.0%
GTCTTTGCAATGAAGTTGTC+TGG - chr1_4:21315190-21315209 None:intergenic 40.0%
! GGAAAGTTGTGTCACTCATA+TGG - chr1_4:21315252-21315271 None:intergenic 40.0%
!!! GATCTTTTCTAGGACCAACT+GGG - chr1_4:21315390-21315409 None:intergenic 40.0%
!!! AGATCTTTTCTAGGACCAAC+TGG - chr1_4:21315391-21315410 None:intergenic 40.0%
GAAACCCAATGGACACAATT+AGG - chr1_4:21315629-21315648 None:intergenic 40.0%
!! AACACAATGAAGGTGTTGGA+GGG - chr1_4:21315662-21315681 None:intergenic 40.0%
TCCAACACCTTCATTGTGTT+GGG + chr1_4:21315662-21315681 Msa0155740:intron 40.0%
CTTCAAACCCAACACAATGA+AGG - chr1_4:21315672-21315691 None:intergenic 40.0%
CTTCATTGTGTTGGGTTTGA+AGG + chr1_4:21315670-21315689 Msa0155740:intron 40.0%
TCATTGTGTTGGGTTTGAAG+GGG + chr1_4:21315672-21315691 Msa0155740:intron 40.0%
GGTCATATCTCTAAGTGATG+TGG - chr1_4:21315709-21315728 None:intergenic 40.0%
!! AAAATCGGCTTGTAAGGTGA+GGG - chr1_4:21315756-21315775 None:intergenic 40.0%
!! CCTTACAAAATCGGCTTGTA+AGG - chr1_4:21315762-21315781 None:intergenic 40.0%
!! CCTTACAAGCCGATTTTGTA+AGG + chr1_4:21315759-21315778 Msa0155740:intron 40.0%
ACCATAAAAAAGGGGAATGG+AGG - chr1_4:21315906-21315925 None:intergenic 40.0%
! AAATGGTGGAAACCACATTG+CGG - chr1_4:21315996-21316015 None:intergenic 40.0%
CAAATACTGTCACGGGAAAA+TGG - chr1_4:21316013-21316032 None:intergenic 40.0%
AACACGATCTTGATCAGAGT+AGG + chr1_4:21316139-21316158 Msa0155740:intron 40.0%
AATACACACCATGGAGAAAC+AGG - chr1_4:21316421-21316440 None:intergenic 40.0%
TGGCACATTCAATCTGTCTT+GGG - chr1_4:21316604-21316623 None:intergenic 40.0%
GTAATTGGTGCAAACTACAG+AGG + chr1_4:21316769-21316788 Msa0155740:intron 40.0%
GTTTCTCCCTAATTGCTAAC+AGG + chr1_4:21316947-21316966 Msa0155740:intron 40.0%
TGCAACTTGAATTGCTTGCA+GGG - chr1_4:21317351-21317370 None:intergenic 40.0%
TTGGGGAAAAAGGTAGAGCT+AGG + chr1_4:21313433-21313452 Msa0155740:five_prime_UTR 45.0%
AGGAACCCACCTTGATACTA+AGG - chr1_4:21313517-21313536 None:intergenic 45.0%
TGAATGAGGGATGAAGGAAG+AGG - chr1_4:21313537-21313556 None:intergenic 45.0%
CCGACACAGTGAAAAACCTT+AGG + chr1_4:21314253-21314272 Msa0155740:intron 45.0%
TTACAATGTAGGCAGGCAAG+TGG + chr1_4:21314282-21314301 Msa0155740:intron 45.0%
TACAATGTAGGCAGGCAAGT+GGG + chr1_4:21314283-21314302 Msa0155740:intron 45.0%
TTACCGACACCCGAGTAATT+TGG + chr1_4:21314376-21314395 Msa0155740:intron 45.0%
ACTGCGTCTGCCAAATTACT+CGG - chr1_4:21314389-21314408 None:intergenic 45.0%
TCACAGTGAGTTCCCAAATC+GGG - chr1_4:21314562-21314581 None:intergenic 45.0%
CTCACAGTGAGTTCCCAAAT+CGG - chr1_4:21314563-21314582 None:intergenic 45.0%
! TCTTTAAGCACCGCACAAGT+TGG - chr1_4:21315005-21315024 None:intergenic 45.0%
GTGCGGTGCTTAAAGATATC+AGG + chr1_4:21315009-21315028 Msa0155740:intron 45.0%
AGTCGCCTAATTGTGTCCAT+TGG + chr1_4:21315621-21315640 Msa0155740:intron 45.0%
GTCGCCTAATTGTGTCCATT+GGG + chr1_4:21315622-21315641 Msa0155740:intron 45.0%
GTGCAATGAAGGAAACCCAA+TGG - chr1_4:21315640-21315659 None:intergenic 45.0%
!! CAACACAATGAAGGTGTTGG+AGG - chr1_4:21315663-21315682 None:intergenic 45.0%
CTCCAACACCTTCATTGTGT+TGG + chr1_4:21315661-21315680 Msa0155740:intron 45.0%
! ACCCAACACAATGAAGGTGT+TGG - chr1_4:21315666-21315685 None:intergenic 45.0%
!! CAAAATCGGCTTGTAAGGTG+AGG - chr1_4:21315757-21315776 None:intergenic 45.0%
AGTTAGGCCCCGATATAAAC+CGG + chr1_4:21315785-21315804 Msa0155740:intron 45.0%
! TCACAAGCCGGTTTATATCG+GGG - chr1_4:21315795-21315814 None:intergenic 45.0%
! CTCACAAGCCGGTTTATATC+GGG - chr1_4:21315796-21315815 None:intergenic 45.0%
! CCTCACAAGCCGGTTTATAT+CGG - chr1_4:21315797-21315816 None:intergenic 45.0%
! TACGTACATCGGTTGAGAGA+TGG - chr1_4:21315855-21315874 None:intergenic 45.0%
!!! GCCTCCATTCCCCTTTTTTA+TGG + chr1_4:21315902-21315921 Msa0155740:intron 45.0%
GACTTCTTTCAACCGCAATG+TGG + chr1_4:21315981-21316000 Msa0155740:intron 45.0%
ATACTGTCACGGGAAAATGG+TGG - chr1_4:21316010-21316029 None:intergenic 45.0%
CTTGATCAGAGTAGGTGAGT+AGG + chr1_4:21316147-21316166 Msa0155740:intron 45.0%
GTGGCACATTCAATCTGTCT+TGG - chr1_4:21316605-21316624 None:intergenic 45.0%
!! GTGTTGTTTGAGCAGTACTG+TGG - chr1_4:21316624-21316643 None:intergenic 45.0%
TATACGTGTGTGTGTGTGTG+TGG + chr1_4:21316904-21316923 Msa0155740:intron 45.0%
! GTGTGTGGTTTTCGATGACA+TGG + chr1_4:21316919-21316938 Msa0155740:intron 45.0%
GTGCAACTTGAATTGCTTGC+AGG - chr1_4:21317352-21317371 None:intergenic 45.0%
AGGTAGAGCTAGGATTTGGG+AGG + chr1_4:21313443-21313462 Msa0155740:five_prime_UTR 50.0%
!! GTGTCGGTTACAATGTAGGC+AGG + chr1_4:21314275-21314294 Msa0155740:intron 50.0%
CTGCCAAATTACTCGGGTGT+CGG - chr1_4:21314382-21314401 None:intergenic 50.0%
CTGCGTCTGCCAAATTACTC+GGG - chr1_4:21314388-21314407 None:intergenic 50.0%
!!! GTGAAGGTTTTGGAGCTTGC+AGG + chr1_4:21315340-21315359 Msa0155740:CDS 50.0%
!!! AAGGTTTTGGAGCTTGCAGG+AGG + chr1_4:21315343-21315362 Msa0155740:CDS 50.0%
CACAATTAGGCGACTAGAGG+CGG - chr1_4:21315616-21315635 None:intergenic 50.0%
GGACACAATTAGGCGACTAG+AGG - chr1_4:21315619-21315638 None:intergenic 50.0%
TTGTGTTGGGTTTGAAGGGG+TGG + chr1_4:21315675-21315694 Msa0155740:intron 50.0%
!! CCGATATAAACCGGCTTGTG+AGG + chr1_4:21315794-21315813 Msa0155740:intron 50.0%
!! ATAAACCGGCTTGTGAGGTG+AGG + chr1_4:21315799-21315818 Msa0155740:intron 50.0%
TTTCATCACGCAACGCCACT+TGG - chr1_4:21317105-21317124 None:intergenic 50.0%
CTAGAGGCGGAGTCCAATGA+AGG - chr1_4:21315603-21315622 None:intergenic 55.0%
! GGTGTTGGAGGGTGCAATGA+AGG - chr1_4:21315651-21315670 None:intergenic 55.0%
GCAATCCTCACCTCACAAGC+CGG - chr1_4:21315807-21315826 None:intergenic 55.0%
GGAGCTTGCAGGAGGAGTGA+TGG + chr1_4:21315351-21315370 Msa0155740:CDS 60.0%
GACGAGCTTGCAAGCCCAGT+TGG + chr1_4:21315373-21315392 Msa0155740:CDS 60.0%
GTAGGCAGGCAAGTGGGACG+CGG + chr1_4:21314289-21314308 Msa0155740:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 21313214 21317551 21313214 ID=Msa0155740;Name=Msa0155740
chr1_4 mRNA 21313214 21317551 21313214 ID=Msa0155740-mRNA-1;Parent=Msa0155740;Name=Msa0155740-mRNA-1;_AED=0.15;_eAED=0.15;_QI=346|1|1|1|0.66|0.5|4|278|115
chr1_4 exon 21313214 21313526 21313214 ID=Msa0155740-mRNA-1:exon:7709;Parent=Msa0155740-mRNA-1
chr1_4 exon 21315140 21315226 21315140 ID=Msa0155740-mRNA-1:exon:7710;Parent=Msa0155740-mRNA-1
chr1_4 exon 21315334 21315450 21315334 ID=Msa0155740-mRNA-1:exon:7711;Parent=Msa0155740-mRNA-1
chr1_4 exon 21317097 21317551 21317097 ID=Msa0155740-mRNA-1:exon:7712;Parent=Msa0155740-mRNA-1
chr1_4 five_prime_UTR 21313214 21313526 21313214 ID=Msa0155740-mRNA-1:five_prime_utr;Parent=Msa0155740-mRNA-1
chr1_4 five_prime_UTR 21315140 21315172 21315140 ID=Msa0155740-mRNA-1:five_prime_utr;Parent=Msa0155740-mRNA-1
chr1_4 CDS 21315173 21315226 21315173 ID=Msa0155740-mRNA-1:cds;Parent=Msa0155740-mRNA-1
chr1_4 CDS 21315334 21315450 21315334 ID=Msa0155740-mRNA-1:cds;Parent=Msa0155740-mRNA-1
chr1_4 CDS 21317097 21317273 21317097 ID=Msa0155740-mRNA-1:cds;Parent=Msa0155740-mRNA-1
chr1_4 three_prime_UTR 21317274 21317551 21317274 ID=Msa0155740-mRNA-1:three_prime_utr;Parent=Msa0155740-mRNA-1
Gene Sequence

>Msa0155740

ATGGATTCACAGGGCCAGACAACTTCATTGCAAAGACTTCAGAATGTCGAAAAGAGAATTGTGAAGGTTTTGGAGCTTGCAGGAGGAGTGATGGACGAGCTTGCAAGCCCAGTTGGTCCTAGAAAAGATCTTGTCCAAAATCACTGTCTTGAATTCATGCAATTAATTAAGGACATCCAAGTGGCGTTGCGTGATGAAATCAAAAGTGCTTGTGAATATCGTCCATTTGAAAAGTGTGATTATGGCCCAAGAATAGCAAATGAGATATGTTTCAAAAAGGTGGAATATGTCATGTCACAATTGGAAGCAATGAAACAAACTATAGATGAGTATAATGCAGCAGCCTGA
Protein sequence

>Msa0155740

MDSQGQTTSLQRLQNVEKRIVKVLELAGGVMDELASPVGPRKDLVQNHCLEFMQLIKDIQVALRDEIKSACEYRPFEKCDYGPRIANEICFKKVEYVMSQLEAMKQTIDEYNAAA*