AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0003130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003130 MtrunA17_Chr1g0155861 98.261 115 2 0 1 115 1 115 2.46e-82 236
Msa0003130 MtrunA17_Chr8g0392451 97.391 115 3 0 1 115 1 115 7.81e-81 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003130 AT3G01435.1 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0003130 AT3G01435.4 73.684 114 30 0 1 114 1 114 6.20e-61 182
Msa0003130 AT3G01435.2 57.534 146 30 1 1 114 1 146 5.45e-54 166
Msa0003130 AT3G01435.3 70.238 84 25 0 31 114 1 84 6.96e-42 133

Find 55 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTCAAGACAGTGATTT+TGG 0.154549 1_1:-5690521 None:intergenic
GGTTAGGGTTCTTTGAAAAT+TGG 0.180356 1_1:+5688521 Msa0003130:five_prime_UTR
CATATCTCATTTGCTATTCT+TGG 0.183234 1_1:-5692279 None:intergenic
TGAAAATTCAGTCTGTAATT+TGG 0.197049 1_1:+5692421 Msa0003130:three_prime_UTR
TGAATTCATGCAATTAATTA+AGG 0.202211 1_1:+5690536 Msa0003130:CDS
GACTTTCTTGGAAGAATATT+GGG 0.218800 1_1:-5688398 None:intergenic
TGACTTTCTTGGAAGAATAT+TGG 0.221095 1_1:-5688399 None:intergenic
TCAAATGCATATGACTTTCT+TGG 0.235673 1_1:-5688410 None:intergenic
GTTAGGGTTCTTTGAAAATT+GGG 0.254874 1_1:+5688522 Msa0003130:five_prime_UTR
AAAGACAGATTATGAAGTTT+TGG 0.264512 1_1:+5692569 Msa0003130:three_prime_UTR
CCATTTGAAAAGTGTGATTA+TGG 0.272416 1_1:+5692255 Msa0003130:CDS
AAGCTTATTTAATTGTCTAA+TGG 0.277330 1_1:-5688451 None:intergenic
ATAGAGAATTGTGAAGGTTT+TGG 0.292105 1_1:+5690437 Msa0003130:intron
AGCTTATTTAATTGTCTAAT+GGG 0.310583 1_1:-5688450 None:intergenic
GGGAGGAAATAGAGTAAATA+AGG 0.319714 1_1:+5688567 Msa0003130:five_prime_UTR
GTGCAACTTGAATTGCTTGC+AGG 0.330825 1_1:-5692456 None:intergenic
GGAGAAAGACTATTAAGGTT+AGG 0.339063 1_1:+5688505 Msa0003130:five_prime_UTR
TCTTTGAAAATTGGGGAAAA+AGG 0.342786 1_1:+5688530 Msa0003130:five_prime_UTR
ATTTATTTCGAGGGGTCATT+TGG 0.349026 1_1:+5692506 Msa0003130:three_prime_UTR
ACCAAGGAGAAAGACTATTA+AGG 0.372416 1_1:+5688500 Msa0003130:five_prime_UTR
AAAAGGTAGAGCTAGGATTT+GGG 0.386260 1_1:+5688547 Msa0003130:five_prime_UTR
ATAAGCTTAGTGAAAGTGAT+TGG 0.391447 1_1:+5688466 Msa0003130:five_prime_UTR
AAATGAGATATGTTTCAAAA+AGG 0.396887 1_1:+5692290 Msa0003130:CDS
GATGGTGATGTTTGAGTGTT+TGG 0.396997 1_1:-5688364 None:intergenic
ATATCTCATTTGCTATTCTT+GGG 0.398050 1_1:-5692278 None:intergenic
ATTGTTAATTGTTAAGAGAA+AGG 0.407894 1_1:+5688308 None:intergenic
GAGAAAGACTATTAAGGTTA+GGG 0.417995 1_1:+5688506 Msa0003130:five_prime_UTR
ACCTTAATAGTCTTTCTCCT+TGG 0.422251 1_1:-5688501 None:intergenic
GGAATATGTCATGTCACAAT+TGG 0.423342 1_1:+5692314 Msa0003130:CDS
AGCAACATCAATGGATTCAC+AGG 0.432506 1_1:+5690270 Msa0003130:exon
TTTATTTCGAGGGGTCATTT+GGG 0.435975 1_1:+5692507 Msa0003130:three_prime_UTR
TTGTTAATTGTTAAGAGAAA+GGG 0.451821 1_1:+5688309 None:intergenic
GTCTTTGCAATGAAGTTGTC+TGG 0.456591 1_1:-5690294 None:intergenic
AACTTTCATCCTTAGTATCA+AGG 0.457524 1_1:+5688612 Msa0003130:five_prime_UTR
TTTCATCACGCAACGCCACT+TGG 0.479312 1_1:-5692209 None:intergenic
AGGAACCCACCTTGATACTA+AGG 0.497854 1_1:-5688621 None:intergenic
ATAGAGTAAATAAGGAACAT+TGG 0.498437 1_1:+5688575 Msa0003130:five_prime_UTR
TATTTATGTATTTATTTCGA+GGG 0.499343 1_1:+5692497 Msa0003130:three_prime_UTR
TTGATACTAAGGATGAAAGT+TGG 0.502389 1_1:-5688610 None:intergenic
TTATTTATGTATTTATTTCG+AGG 0.502816 1_1:+5692496 Msa0003130:three_prime_UTR
CACAGAGAAAGCAACATCAA+TGG 0.506024 1_1:+5690261 Msa0003130:exon
TTAGGGTTCTTTGAAAATTG+GGG 0.511126 1_1:+5688523 Msa0003130:five_prime_UTR
ATGTCTTTAATCTCAAACAT+AGG 0.521066 1_1:-5692640 None:intergenic
TGCAACTTGAATTGCTTGCA+GGG 0.527566 1_1:-5692455 None:intergenic
TTGTCAATTGTGAACTATCT+CGG 0.532804 1_1:+5688341 Msa0003130:five_prime_UTR
GACGAGCTTGCAAGCCCAGT+TGG 0.535324 1_1:+5690480 Msa0003130:CDS
AAGACTTCAGAATGTCGAAA+AGG 0.556491 1_1:+5690312 Msa0003130:CDS
GGAGCTTGCAGGAGGAGTGA+TGG 0.557814 1_1:+5690458 Msa0003130:CDS
ACTTTCTTGGAAGAATATTG+GGG 0.595084 1_1:-5688397 None:intergenic
AGGTAGAGCTAGGATTTGGG+AGG 0.607159 1_1:+5688550 Msa0003130:five_prime_UTR
AATTCAAGTTGCACTCAACA+AGG 0.612180 1_1:+5692465 Msa0003130:three_prime_UTR
ATTTATGTATTTATTTCGAG+GGG 0.636208 1_1:+5692498 Msa0003130:three_prime_UTR
GTTTGTATAGAGAATTGTGA+AGG 0.675616 1_1:+5690431 Msa0003130:intron
ATTGGAGAGTAAATCAACCA+AGG 0.682126 1_1:+5688484 Msa0003130:five_prime_UTR
GCAACATCAATGGATTCACA+GGG 0.701218 1_1:+5690271 Msa0003130:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAGAATTATGAAAT+TGG + chr1_1:5688670-5688689 Msa0003130:intron 10.0%
!! ATATTAAAGTATATTAAAGA+AGG + chr1_1:5689543-5689562 Msa0003130:intron 10.0%
!! TATGATATTAATTATTGAAA+TGG - chr1_1:5690640-5690659 None:intergenic 10.0%
!! ATAATTTATCAAATGAATGA+GGG - chr1_1:5688657-5688676 None:intergenic 15.0%
!! TATAATTTATCAAATGAATG+AGG - chr1_1:5688658-5688677 None:intergenic 15.0%
!! TATAGTCTATAATTATTAAG+AGG - chr1_1:5689143-5689162 None:intergenic 15.0%
!! AATAAAGAAATCAACATAAT+GGG - chr1_1:5689708-5689727 None:intergenic 15.0%
!! TAATAAAGAAATCAACATAA+TGG - chr1_1:5689709-5689728 None:intergenic 15.0%
!!! ATTATGTTGATTTCTTTATT+AGG + chr1_1:5689708-5689727 Msa0003130:intron 15.0%
!! TAAAAAAAATCAAGTATGAA+AGG - chr1_1:5690380-5690399 None:intergenic 15.0%
!! ATGAATAAGTGTTTATAAAT+GGG - chr1_1:5690938-5690957 None:intergenic 15.0%
!!! TGATTTTCAATTACAATTAA+CGG + chr1_1:5691345-5691364 Msa0003130:intron 15.0%
!! TTCATTTCATATCTTTAATT+TGG - chr1_1:5691798-5691817 None:intergenic 15.0%
!!! AAACTATTTTTGATAGAAAT+GGG - chr1_1:5691823-5691842 None:intergenic 15.0%
!!! TAAACTATTTTTGATAGAAA+TGG - chr1_1:5691824-5691843 None:intergenic 15.0%
!! AATATTACAACATTATAGTA+GGG - chr1_1:5692133-5692152 None:intergenic 15.0%
!! AAAAATTAGAAAGAAAATGA+AGG - chr1_1:5692180-5692199 None:intergenic 15.0%
!!! ATTTTCTTTCTAATTTTTGT+AGG + chr1_1:5692182-5692201 Msa0003130:intron 15.0%
!! TTATTTATGTATTTATTTCG+AGG + chr1_1:5692496-5692515 Msa0003130:three_prime_UTR 15.0%
!! TATTTATGTATTTATTTCGA+GGG + chr1_1:5692497-5692516 Msa0003130:three_prime_UTR 15.0%
!! AGCTTATTTAATTGTCTAAT+GGG - chr1_1:5688453-5688472 None:intergenic 20.0%
!! AAGCTTATTTAATTGTCTAA+TGG - chr1_1:5688454-5688473 None:intergenic 20.0%
!! AGAATTATGAAATTGGAATT+TGG + chr1_1:5688677-5688696 Msa0003130:intron 20.0%
!! GAATTATGAAATTGGAATTT+GGG + chr1_1:5688678-5688697 Msa0003130:intron 20.0%
!! TTAGTAATATTCATATCTAG+TGG - chr1_1:5689241-5689260 None:intergenic 20.0%
!! TACTTGATAAAAATCTTACA+CGG - chr1_1:5689892-5689911 None:intergenic 20.0%
!! AAAATCTCTAAACAAATTTG+TGG + chr1_1:5689942-5689961 Msa0003130:intron 20.0%
!! AAGATATCAGGAAAAAAATT+AGG + chr1_1:5690128-5690147 Msa0003130:intron 20.0%
!! TGAATTCATGCAATTAATTA+AGG + chr1_1:5690536-5690555 Msa0003130:CDS 20.0%
!!! TAGTTGTTACACAATAATTT+TGG - chr1_1:5690675-5690694 None:intergenic 20.0%
!! AATTATTGTGTAACAACTAT+TGG + chr1_1:5690676-5690695 Msa0003130:intron 20.0%
!! TGAATAAGTGTTTATAAATG+GGG - chr1_1:5690937-5690956 None:intergenic 20.0%
!! CATGAATAAGTGTTTATAAA+TGG - chr1_1:5690939-5690958 None:intergenic 20.0%
!! TTCAATATATAAACTATGCT+TGG + chr1_1:5690987-5691006 Msa0003130:intron 20.0%
!! ACTCAATTACACAAATTTAA+AGG - chr1_1:5691456-5691475 None:intergenic 20.0%
!! GAATATTACAACATTATAGT+AGG - chr1_1:5692134-5692153 None:intergenic 20.0%
!! AAATGAGATATGTTTCAAAA+AGG + chr1_1:5692290-5692309 Msa0003130:CDS 20.0%
!!! ATTTAAGAAGCAAAATTTTC+AGG - chr1_1:5692379-5692398 None:intergenic 20.0%
!! ATTTATGTATTTATTTCGAG+GGG + chr1_1:5692498-5692517 Msa0003130:three_prime_UTR 20.0%
! ATAGAGTAAATAAGGAACAT+TGG + chr1_1:5688575-5688594 Msa0003130:five_prime_UTR 25.0%
!! ATAATATGATGATGTCCAAA+TGG - chr1_1:5689002-5689021 None:intergenic 25.0%
!!! TAGTTGATGTTTTTTATCAG+TGG + chr1_1:5689161-5689180 Msa0003130:intron 25.0%
! TTTATCAGTGGAATGAATAT+TGG + chr1_1:5689173-5689192 Msa0003130:intron 25.0%
!! CTAGTAGAAGATATTGATTA+AGG + chr1_1:5689264-5689283 Msa0003130:intron 25.0%
! ATTGTTATAGCATACTACAT+TGG + chr1_1:5689441-5689460 Msa0003130:intron 25.0%
!! ATTGGCAGTACAAATTATTT+TGG + chr1_1:5689459-5689478 Msa0003130:intron 25.0%
!! ATTTCCCTTTTTGTAAAATG+TGG + chr1_1:5689807-5689826 Msa0003130:intron 25.0%
!! TTTTCTAAGCAGTAAACTAA+TGG - chr1_1:5690014-5690033 None:intergenic 25.0%
! AATCTTGCCAAAATATTGTA+GGG - chr1_1:5690176-5690195 None:intergenic 25.0%
! TAATCTTGCCAAAATATTGT+AGG - chr1_1:5690177-5690196 None:intergenic 25.0%
!! TTTTTATTACAAGATTGCAC+TGG + chr1_1:5690589-5690608 Msa0003130:intron 25.0%
! TATATAACTATAGATGCAAG+TGG + chr1_1:5690612-5690631 Msa0003130:intron 25.0%
! GAATAAGTGTTTATAAATGG+GGG - chr1_1:5690936-5690955 None:intergenic 25.0%
! TATGTTGCATACAAATGAAA+GGG - chr1_1:5691047-5691066 None:intergenic 25.0%
!!! CTATTTCACATAACTGATTT+TGG + chr1_1:5691140-5691159 Msa0003130:intron 25.0%
! GACTATTAGCACTTAAAAAA+TGG + chr1_1:5691485-5691504 Msa0003130:intron 25.0%
! TTCAAATACCAAACTTACAA+AGG - chr1_1:5691619-5691638 None:intergenic 25.0%
! TAATGAACATTAGATTTCTC+AGG - chr1_1:5691643-5691662 None:intergenic 25.0%
! CTATCAAAAATAGTTTACCT+TGG + chr1_1:5691827-5691846 Msa0003130:intron 25.0%
! AAACAACTAAGTACATGTTT+GGG + chr1_1:5691854-5691873 Msa0003130:intron 25.0%
! AACACAAATAATTCTTCTAG+AGG - chr1_1:5691934-5691953 None:intergenic 25.0%
! TATATACTCCAATAACTAAC+AGG - chr1_1:5691995-5692014 None:intergenic 25.0%
!!! ATATCTCATTTGCTATTCTT+GGG - chr1_1:5692281-5692300 None:intergenic 25.0%
! TGAAAATTCAGTCTGTAATT+TGG + chr1_1:5692421-5692440 Msa0003130:three_prime_UTR 25.0%
!! TAATCTGTCTTTTACATGTT+TGG - chr1_1:5692561-5692580 None:intergenic 25.0%
!! AAAGACAGATTATGAAGTTT+TGG + chr1_1:5692569-5692588 Msa0003130:three_prime_UTR 25.0%
TTGTCAATTGTGAACTATCT+CGG + chr1_1:5688341-5688360 Msa0003130:five_prime_UTR 30.0%
ACTTTCTTGGAAGAATATTG+GGG - chr1_1:5688400-5688419 None:intergenic 30.0%
GACTTTCTTGGAAGAATATT+GGG - chr1_1:5688401-5688420 None:intergenic 30.0%
TGACTTTCTTGGAAGAATAT+TGG - chr1_1:5688402-5688421 None:intergenic 30.0%
TCAAATGCATATGACTTTCT+TGG - chr1_1:5688413-5688432 None:intergenic 30.0%
ATAAGCTTAGTGAAAGTGAT+TGG + chr1_1:5688466-5688485 Msa0003130:five_prime_UTR 30.0%
GAGAAAGACTATTAAGGTTA+GGG + chr1_1:5688506-5688525 Msa0003130:five_prime_UTR 30.0%
! GTTAGGGTTCTTTGAAAATT+GGG + chr1_1:5688522-5688541 Msa0003130:five_prime_UTR 30.0%
! TTAGGGTTCTTTGAAAATTG+GGG + chr1_1:5688523-5688542 Msa0003130:five_prime_UTR 30.0%
! TCTTTGAAAATTGGGGAAAA+AGG + chr1_1:5688530-5688549 Msa0003130:five_prime_UTR 30.0%
TTGATACTAAGGATGAAAGT+TGG - chr1_1:5688613-5688632 None:intergenic 30.0%
AACTTTCATCCTTAGTATCA+AGG + chr1_1:5688612-5688631 Msa0003130:five_prime_UTR 30.0%
! AAAGCATTCAAATACATCGA+AGG - chr1_1:5688877-5688896 None:intergenic 30.0%
CAGATTAAGATTGCACATTA+AGG + chr1_1:5688968-5688987 Msa0003130:intron 30.0%
ATTAAGGTCATAACTCCATT+TGG + chr1_1:5688984-5689003 Msa0003130:intron 30.0%
AAGTGAATAAGAATGAAGTC+TGG + chr1_1:5689037-5689056 Msa0003130:intron 30.0%
CAACAGATTGTATCGAAAAT+AGG + chr1_1:5689094-5689113 Msa0003130:intron 30.0%
AAATAGGATAGAACATGCAA+TGG + chr1_1:5689110-5689129 Msa0003130:intron 30.0%
TAGGTAGTGTTTAGATAATG+TGG + chr1_1:5689310-5689329 Msa0003130:intron 30.0%
AGGTAGTGTTTAGATAATGT+GGG + chr1_1:5689311-5689330 Msa0003130:intron 30.0%
CAATAATAAAGCTTTGCAAG+TGG - chr1_1:5689425-5689444 None:intergenic 30.0%
! ACTAGAAATTGATTCCAACT+TGG + chr1_1:5689629-5689648 Msa0003130:intron 30.0%
ATAAACATAGAATGCCAAGT+TGG - chr1_1:5689646-5689665 None:intergenic 30.0%
ATTCTATGTTTATCCCGATT+TGG + chr1_1:5689653-5689672 Msa0003130:intron 30.0%
TTCTATGTTTATCCCGATTT+GGG + chr1_1:5689654-5689673 Msa0003130:intron 30.0%
!! TAAGCTATTTTGCTGTCAAT+CGG + chr1_1:5689733-5689752 Msa0003130:intron 30.0%
! TCAGCCACATTTTACAAAAA+GGG - chr1_1:5689814-5689833 None:intergenic 30.0%
! ATCAGCCACATTTTACAAAA+AGG - chr1_1:5689815-5689834 None:intergenic 30.0%
TTGATAAAAATCTTACACGG+CGG - chr1_1:5689889-5689908 None:intergenic 30.0%
! AACTAATGGTGTGTTTGTTT+TGG - chr1_1:5690000-5690019 None:intergenic 30.0%
GTTTGTATAGAGAATTGTGA+AGG + chr1_1:5690431-5690450 Msa0003130:intron 30.0%
! ATAGAGAATTGTGAAGGTTT+TGG + chr1_1:5690437-5690456 Msa0003130:intron 30.0%
! TTTTGGACAAGATCTTTTCT+AGG - chr1_1:5690507-5690526 None:intergenic 30.0%
! TGAATTCAAGACAGTGATTT+TGG - chr1_1:5690524-5690543 None:intergenic 30.0%
AATTATTGAGAGACAAAGCA+AGG - chr1_1:5690566-5690585 None:intergenic 30.0%
CTAACTCATCCTTACAAAAT+CGG - chr1_1:5690878-5690897 None:intergenic 30.0%
ATTGAAATGTCTACGTACAT+CGG - chr1_1:5690973-5690992 None:intergenic 30.0%
ATGGGAATCACCATAAAAAA+GGG - chr1_1:5691022-5691041 None:intergenic 30.0%
TATGGGAATCACCATAAAAA+AGG - chr1_1:5691023-5691042 None:intergenic 30.0%
CATACAAATGAAAGGGTTAT+GGG - chr1_1:5691040-5691059 None:intergenic 30.0%
GTATGTTGCATACAAATGAA+AGG - chr1_1:5691048-5691067 None:intergenic 30.0%
TGAAATAGCAAATACTGTCA+CGG - chr1_1:5691128-5691147 None:intergenic 30.0%
ATGTAATCAATAGCAGATTG+CGG - chr1_1:5691177-5691196 None:intergenic 30.0%
AACAGATAGAATTGTGATCA+AGG - chr1_1:5691326-5691345 None:intergenic 30.0%
TACCTAAAAAGAGGATAAAG+CGG - chr1_1:5691379-5691398 None:intergenic 30.0%
CAGATTTGATACCTAAAAAG+AGG - chr1_1:5691388-5691407 None:intergenic 30.0%
TATCACAACCAAACTTACAA+AGG - chr1_1:5691588-5691607 None:intergenic 30.0%
AAACAACACTAGTTATGTGA+TGG + chr1_1:5691742-5691761 Msa0003130:intron 30.0%
CAAACATGTACTTAGTTGTT+TGG - chr1_1:5691855-5691874 None:intergenic 30.0%
CAAACAACTAAGTACATGTT+TGG + chr1_1:5691853-5691872 Msa0003130:intron 30.0%
TAAGTACATGTTTGGGTAAT+TGG + chr1_1:5691861-5691880 Msa0003130:intron 30.0%
ACTAAACCTGTTAGCAATTA+GGG - chr1_1:5692063-5692082 None:intergenic 30.0%
AACTAAACCTGTTAGCAATT+AGG - chr1_1:5692064-5692083 None:intergenic 30.0%
CTAACAGGTTTAGTTATTCA+GGG + chr1_1:5692069-5692088 Msa0003130:intron 30.0%
! CCATAATCACACTTTTCAAA+TGG - chr1_1:5692258-5692277 None:intergenic 30.0%
! CCATTTGAAAAGTGTGATTA+TGG + chr1_1:5692255-5692274 Msa0003130:CDS 30.0%
!! CATATCTCATTTGCTATTCT+TGG - chr1_1:5692282-5692301 None:intergenic 30.0%
TGAGATATGTTTCAAAAAGG+TGG + chr1_1:5692293-5692312 Msa0003130:CDS 30.0%
TATTGGGGAAAAAGCTATGA+TGG - chr1_1:5688385-5688404 None:intergenic 35.0%
ATTGGAGAGTAAATCAACCA+AGG + chr1_1:5688484-5688503 Msa0003130:five_prime_UTR 35.0%
ACCAAGGAGAAAGACTATTA+AGG + chr1_1:5688500-5688519 Msa0003130:five_prime_UTR 35.0%
ACCTTAATAGTCTTTCTCCT+TGG - chr1_1:5688504-5688523 None:intergenic 35.0%
GGAGAAAGACTATTAAGGTT+AGG + chr1_1:5688505-5688524 Msa0003130:five_prime_UTR 35.0%
! GGTTAGGGTTCTTTGAAAAT+TGG + chr1_1:5688521-5688540 Msa0003130:five_prime_UTR 35.0%
AAAAAGGTAGAGCTAGGATT+TGG + chr1_1:5688546-5688565 Msa0003130:five_prime_UTR 35.0%
AAAAGGTAGAGCTAGGATTT+GGG + chr1_1:5688547-5688566 Msa0003130:five_prime_UTR 35.0%
GGGAGGAAATAGAGTAAATA+AGG + chr1_1:5688567-5688586 Msa0003130:five_prime_UTR 35.0%
TTTCATCCTTAGTATCAAGG+TGG + chr1_1:5688615-5688634 Msa0003130:intron 35.0%
TTCATCCTTAGTATCAAGGT+GGG + chr1_1:5688616-5688635 Msa0003130:intron 35.0%
ATCAAATGAATGAGGGATGA+AGG - chr1_1:5688650-5688669 None:intergenic 35.0%
ATGTGCAATCTTAATCTGCA+AGG - chr1_1:5688966-5688985 None:intergenic 35.0%
ATACAGTGTGAACCTGATAT+AGG + chr1_1:5689209-5689228 Msa0003130:intron 35.0%
TACAGTGTGAACCTGATATA+GGG + chr1_1:5689210-5689229 Msa0003130:intron 35.0%
TAGTTGCAATTTGTGCAACT+AGG + chr1_1:5689291-5689310 Msa0003130:intron 35.0%
GGTAGTGTTTAGATAATGTG+GGG + chr1_1:5689312-5689331 Msa0003130:intron 35.0%
!! TTTTGCTGTCAATCGGATAT+CGG + chr1_1:5689740-5689759 Msa0003130:intron 35.0%
! TGTCTAGAGTCTAGAATTCT+CGG + chr1_1:5689771-5689790 Msa0003130:intron 35.0%
AGTGTTGCGTAAAGATTGAT+TGG + chr1_1:5690041-5690060 Msa0003130:intron 35.0%
AAGACTTCAGAATGTCGAAA+AGG + chr1_1:5690312-5690331 Msa0003130:CDS 35.0%
ATTGGAAATTCTGCCTTCAT+TGG + chr1_1:5690694-5690713 Msa0003130:intron 35.0%
TTCATTGTGTTGGGTTTGAA+GGG + chr1_1:5690778-5690797 Msa0003130:intron 35.0%
GTCATATCTCTAAGTGATGT+GGG - chr1_1:5690815-5690834 None:intergenic 35.0%
CCAATGCTTTATAAACACAC+AGG - chr1_1:5690837-5690856 None:intergenic 35.0%
CCTGTGTGTTTATAAAGCAT+TGG + chr1_1:5690834-5690853 Msa0003130:intron 35.0%
! CGATTTTGTAAGGATGAGTT+AGG + chr1_1:5690876-5690895 Msa0003130:intron 35.0%
ATCACCATAAAAAAGGGGAA+TGG - chr1_1:5691016-5691035 None:intergenic 35.0%
TGGGAATCACCATAAAAAAG+GGG - chr1_1:5691021-5691040 None:intergenic 35.0%
GCATACAAATGAAAGGGTTA+TGG - chr1_1:5691041-5691060 None:intergenic 35.0%
GAAATAGCAAATACTGTCAC+GGG - chr1_1:5691127-5691146 None:intergenic 35.0%
! ATCCGCTTTATCCTCTTTTT+AGG + chr1_1:5691374-5691393 Msa0003130:intron 35.0%
! AAAATTGTCCTGTTTCTCCA+TGG + chr1_1:5691517-5691536 Msa0003130:intron 35.0%
AAGCATGAAAATACACACCA+TGG - chr1_1:5691537-5691556 None:intergenic 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_1:5691577-5691596 Msa0003130:intron 35.0%
AACACACTCCTTTGTAAGTT+TGG + chr1_1:5691577-5691596 Msa0003130:intron 35.0%
TACTTAGTTGTTTGGATCCA+AGG - chr1_1:5691847-5691866 None:intergenic 35.0%
TAATTGGTGCAAACTACAGA+GGG + chr1_1:5691877-5691896 Msa0003130:intron 35.0%
TCTTCTGAGTGCATTGTATT+TGG + chr1_1:5691900-5691919 Msa0003130:intron 35.0%
AGTTCTGTCCTGTTAGTTAT+TGG + chr1_1:5691984-5692003 Msa0003130:intron 35.0%
GCTAACAGGTTTAGTTATTC+AGG + chr1_1:5692068-5692087 Msa0003130:intron 35.0%
! ATTTTTGTAGGACATCCAAG+TGG + chr1_1:5692194-5692213 Msa0003130:intron 35.0%
GGAATATGTCATGTCACAAT+TGG + chr1_1:5692314-5692333 Msa0003130:CDS 35.0%
!! AATTCAAGTTGCACTCAACA+AGG + chr1_1:5692465-5692484 Msa0003130:three_prime_UTR 35.0%
ATTTATTTCGAGGGGTCATT+TGG + chr1_1:5692506-5692525 Msa0003130:three_prime_UTR 35.0%
TTTATTTCGAGGGGTCATTT+GGG + chr1_1:5692507-5692526 Msa0003130:three_prime_UTR 35.0%
! GATGGTGATGTTTGAGTGTT+TGG - chr1_1:5688367-5688386 None:intergenic 40.0%
! CTGAGTTGCTTTCATGTGTA+TGG + chr1_1:5688821-5688840 Msa0003130:intron 40.0%
! AGTTGCTTTCATGTGTATGG+AGG + chr1_1:5688824-5688843 Msa0003130:intron 40.0%
CGATACAATCTGTTGCATGA+AGG - chr1_1:5689089-5689108 None:intergenic 40.0%
TAGTGGCATTACCCTATATC+AGG - chr1_1:5689224-5689243 None:intergenic 40.0%
TGGGGAAGATTAGTAAGCTA+AGG + chr1_1:5689330-5689349 Msa0003130:intron 40.0%
! CCTAAGGTTTTTCACTGTGT+CGG - chr1_1:5689363-5689382 None:intergenic 40.0%
CAGTGAAAAACCTTAGGTGT+CGG + chr1_1:5689366-5689385 Msa0003130:intron 40.0%
TACATTGTAACCGACACCTA+AGG - chr1_1:5689379-5689398 None:intergenic 40.0%
!! TTAGGTGTCGGTTACAATGT+AGG + chr1_1:5689378-5689397 Msa0003130:intron 40.0%
! AAGAAGGCATGTGATGCTAA+CGG + chr1_1:5689559-5689578 Msa0003130:intron 40.0%
TCTGCATAATCCAACTTGTG+CGG + chr1_1:5690099-5690118 Msa0003130:intron 40.0%
! TACCGCTCCCTACAATATTT+TGG + chr1_1:5690166-5690185 Msa0003130:intron 40.0%
TGCCAAAATATTGTAGGGAG+CGG - chr1_1:5690171-5690190 None:intergenic 40.0%
CACAGAGAAAGCAACATCAA+TGG + chr1_1:5690261-5690280 Msa0003130:exon 40.0%
! AGCAACATCAATGGATTCAC+AGG + chr1_1:5690270-5690289 Msa0003130:exon 40.0%
! GCAACATCAATGGATTCACA+GGG + chr1_1:5690271-5690290 Msa0003130:exon 40.0%
GTCTTTGCAATGAAGTTGTC+TGG - chr1_1:5690297-5690316 None:intergenic 40.0%
! GGAAAGTTGTGTCACTCATA+TGG - chr1_1:5690359-5690378 None:intergenic 40.0%
!!! GATCTTTTCTAGGACCAACT+GGG - chr1_1:5690497-5690516 None:intergenic 40.0%
!!! AGATCTTTTCTAGGACCAAC+TGG - chr1_1:5690498-5690517 None:intergenic 40.0%
GAAACCCAATGGACACAATT+AGG - chr1_1:5690736-5690755 None:intergenic 40.0%
!! AACACAATGAAGGTGTTGGA+GGG - chr1_1:5690769-5690788 None:intergenic 40.0%
TCCAACACCTTCATTGTGTT+GGG + chr1_1:5690769-5690788 Msa0003130:intron 40.0%
CTTCAAACCCAACACAATGA+AGG - chr1_1:5690779-5690798 None:intergenic 40.0%
CTTCATTGTGTTGGGTTTGA+AGG + chr1_1:5690777-5690796 Msa0003130:intron 40.0%
TCATTGTGTTGGGTTTGAAG+GGG + chr1_1:5690779-5690798 Msa0003130:intron 40.0%
GGTCATATCTCTAAGTGATG+TGG - chr1_1:5690816-5690835 None:intergenic 40.0%
!! AAAATCGGCTTGTAAGGTGA+GGG - chr1_1:5690863-5690882 None:intergenic 40.0%
!! CCTTACAAAATCGGCTTGTA+AGG - chr1_1:5690869-5690888 None:intergenic 40.0%
!! CCTTACAAGCCGATTTTGTA+AGG + chr1_1:5690866-5690885 Msa0003130:intron 40.0%
ACCATAAAAAAGGGGAATGG+AGG - chr1_1:5691013-5691032 None:intergenic 40.0%
! AAATGGTGGAAACCACATTG+CGG - chr1_1:5691103-5691122 None:intergenic 40.0%
CAAATACTGTCACGGGAAAA+TGG - chr1_1:5691120-5691139 None:intergenic 40.0%
AACACGATCTTGATCAGAGT+AGG + chr1_1:5691246-5691265 Msa0003130:intron 40.0%
AATACACACCATGGAGAAAC+AGG - chr1_1:5691528-5691547 None:intergenic 40.0%
TGGCACATTCAATCTGTCTT+GGG - chr1_1:5691711-5691730 None:intergenic 40.0%
GTAATTGGTGCAAACTACAG+AGG + chr1_1:5691876-5691895 Msa0003130:intron 40.0%
GTTTCTCCCTAATTGCTAAC+AGG + chr1_1:5692054-5692073 Msa0003130:intron 40.0%
TGCAACTTGAATTGCTTGCA+GGG - chr1_1:5692458-5692477 None:intergenic 40.0%
TTGGGGAAAAAGGTAGAGCT+AGG + chr1_1:5688540-5688559 Msa0003130:five_prime_UTR 45.0%
AGGAACCCACCTTGATACTA+AGG - chr1_1:5688624-5688643 None:intergenic 45.0%
TGAATGAGGGATGAAGGAAG+AGG - chr1_1:5688644-5688663 None:intergenic 45.0%
CCGACACAGTGAAAAACCTT+AGG + chr1_1:5689360-5689379 Msa0003130:intron 45.0%
TTACAATGTAGGCAGGCAAG+TGG + chr1_1:5689389-5689408 Msa0003130:intron 45.0%
TACAATGTAGGCAGGCAAGT+GGG + chr1_1:5689390-5689409 Msa0003130:intron 45.0%
TTACCGACACCCGAGTAATT+TGG + chr1_1:5689483-5689502 Msa0003130:intron 45.0%
ACTGCGTCTGCCAAATTACT+CGG - chr1_1:5689496-5689515 None:intergenic 45.0%
TCACAGTGAGTTCCCAAATC+GGG - chr1_1:5689669-5689688 None:intergenic 45.0%
CTCACAGTGAGTTCCCAAAT+CGG - chr1_1:5689670-5689689 None:intergenic 45.0%
! TCTTTAAGCACCGCACAAGT+TGG - chr1_1:5690112-5690131 None:intergenic 45.0%
GTGCGGTGCTTAAAGATATC+AGG + chr1_1:5690116-5690135 Msa0003130:intron 45.0%
AGTCGCCTAATTGTGTCCAT+TGG + chr1_1:5690728-5690747 Msa0003130:intron 45.0%
GTCGCCTAATTGTGTCCATT+GGG + chr1_1:5690729-5690748 Msa0003130:intron 45.0%
GTGCAATGAAGGAAACCCAA+TGG - chr1_1:5690747-5690766 None:intergenic 45.0%
!! CAACACAATGAAGGTGTTGG+AGG - chr1_1:5690770-5690789 None:intergenic 45.0%
CTCCAACACCTTCATTGTGT+TGG + chr1_1:5690768-5690787 Msa0003130:intron 45.0%
! ACCCAACACAATGAAGGTGT+TGG - chr1_1:5690773-5690792 None:intergenic 45.0%
!! CAAAATCGGCTTGTAAGGTG+AGG - chr1_1:5690864-5690883 None:intergenic 45.0%
AGTTAGGCCCCGATATAAAC+CGG + chr1_1:5690892-5690911 Msa0003130:intron 45.0%
! TCACAAGCCGGTTTATATCG+GGG - chr1_1:5690902-5690921 None:intergenic 45.0%
! CTCACAAGCCGGTTTATATC+GGG - chr1_1:5690903-5690922 None:intergenic 45.0%
! CCTCACAAGCCGGTTTATAT+CGG - chr1_1:5690904-5690923 None:intergenic 45.0%
! TACGTACATCGGTTGAGAGA+TGG - chr1_1:5690962-5690981 None:intergenic 45.0%
!!! GCCTCCATTCCCCTTTTTTA+TGG + chr1_1:5691009-5691028 Msa0003130:intron 45.0%
GACTTCTTTCAACCGCAATG+TGG + chr1_1:5691088-5691107 Msa0003130:intron 45.0%
ATACTGTCACGGGAAAATGG+TGG - chr1_1:5691117-5691136 None:intergenic 45.0%
CTTGATCAGAGTAGGTGAGT+AGG + chr1_1:5691254-5691273 Msa0003130:intron 45.0%
GTGGCACATTCAATCTGTCT+TGG - chr1_1:5691712-5691731 None:intergenic 45.0%
!! GTGTTGTTTGAGCAGTACTG+TGG - chr1_1:5691731-5691750 None:intergenic 45.0%
TATACGTGTGTGTGTGTGTG+TGG + chr1_1:5692011-5692030 Msa0003130:intron 45.0%
! GTGTGTGGTTTTCGATGACA+TGG + chr1_1:5692026-5692045 Msa0003130:intron 45.0%
GTGCAACTTGAATTGCTTGC+AGG - chr1_1:5692459-5692478 None:intergenic 45.0%
AGGTAGAGCTAGGATTTGGG+AGG + chr1_1:5688550-5688569 Msa0003130:five_prime_UTR 50.0%
!! GTGTCGGTTACAATGTAGGC+AGG + chr1_1:5689382-5689401 Msa0003130:intron 50.0%
CTGCCAAATTACTCGGGTGT+CGG - chr1_1:5689489-5689508 None:intergenic 50.0%
CTGCGTCTGCCAAATTACTC+GGG - chr1_1:5689495-5689514 None:intergenic 50.0%
!!! GTGAAGGTTTTGGAGCTTGC+AGG + chr1_1:5690447-5690466 Msa0003130:CDS 50.0%
!!! AAGGTTTTGGAGCTTGCAGG+AGG + chr1_1:5690450-5690469 Msa0003130:CDS 50.0%
CACAATTAGGCGACTAGAGG+CGG - chr1_1:5690723-5690742 None:intergenic 50.0%
GGACACAATTAGGCGACTAG+AGG - chr1_1:5690726-5690745 None:intergenic 50.0%
TTGTGTTGGGTTTGAAGGGG+TGG + chr1_1:5690782-5690801 Msa0003130:intron 50.0%
!! CCGATATAAACCGGCTTGTG+AGG + chr1_1:5690901-5690920 Msa0003130:intron 50.0%
!! ATAAACCGGCTTGTGAGGTG+AGG + chr1_1:5690906-5690925 Msa0003130:intron 50.0%
TTTCATCACGCAACGCCACT+TGG - chr1_1:5692212-5692231 None:intergenic 50.0%
CTAGAGGCGGAGTCCAATGA+AGG - chr1_1:5690710-5690729 None:intergenic 55.0%
! GGTGTTGGAGGGTGCAATGA+AGG - chr1_1:5690758-5690777 None:intergenic 55.0%
GCAATCCTCACCTCACAAGC+CGG - chr1_1:5690914-5690933 None:intergenic 55.0%
GGAGCTTGCAGGAGGAGTGA+TGG + chr1_1:5690458-5690477 Msa0003130:CDS 60.0%
GACGAGCTTGCAAGCCCAGT+TGG + chr1_1:5690480-5690499 Msa0003130:CDS 60.0%
GTAGGCAGGCAAGTGGGACG+CGG + chr1_1:5689396-5689415 Msa0003130:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 5688321 5692658 5688321 ID=Msa0003130;Name=Msa0003130
chr1_1 mRNA 5688321 5692658 5688321 ID=Msa0003130-mRNA-1;Parent=Msa0003130;Name=Msa0003130-mRNA-1;_AED=0.15;_eAED=0.15;_QI=346|1|1|1|0.66|0.5|4|278|115
chr1_1 exon 5688321 5688633 5688321 ID=Msa0003130-mRNA-1:exon:1704;Parent=Msa0003130-mRNA-1
chr1_1 exon 5690247 5690333 5690247 ID=Msa0003130-mRNA-1:exon:1705;Parent=Msa0003130-mRNA-1
chr1_1 exon 5690441 5690557 5690441 ID=Msa0003130-mRNA-1:exon:1706;Parent=Msa0003130-mRNA-1
chr1_1 exon 5692204 5692658 5692204 ID=Msa0003130-mRNA-1:exon:1707;Parent=Msa0003130-mRNA-1
chr1_1 five_prime_UTR 5688321 5688633 5688321 ID=Msa0003130-mRNA-1:five_prime_utr;Parent=Msa0003130-mRNA-1
chr1_1 five_prime_UTR 5690247 5690279 5690247 ID=Msa0003130-mRNA-1:five_prime_utr;Parent=Msa0003130-mRNA-1
chr1_1 CDS 5690280 5690333 5690280 ID=Msa0003130-mRNA-1:cds;Parent=Msa0003130-mRNA-1
chr1_1 CDS 5690441 5690557 5690441 ID=Msa0003130-mRNA-1:cds;Parent=Msa0003130-mRNA-1
chr1_1 CDS 5692204 5692380 5692204 ID=Msa0003130-mRNA-1:cds;Parent=Msa0003130-mRNA-1
chr1_1 three_prime_UTR 5692381 5692658 5692381 ID=Msa0003130-mRNA-1:three_prime_utr;Parent=Msa0003130-mRNA-1
Gene Sequence

>Msa0003130

ATGGATTCACAGGGCCAGACAACTTCATTGCAAAGACTTCAGAATGTCGAAAAGAGAATTGTGAAGGTTTTGGAGCTTGCAGGAGGAGTGATGGACGAGCTTGCAAGCCCAGTTGGTCCTAGAAAAGATCTTGTCCAAAATCACTGTCTTGAATTCATGCAATTAATTAAGGACATCCAAGTGGCGTTGCGTGATGAAATCAAAAGTGCTTGTGAATATCGTCCATTTGAAAAGTGTGATTATGGCCCAAGAATAGCAAATGAGATATGTTTCAAAAAGGTGGAATATGTCATGTCACAATTGGAAGCAATGAAACAAACTATAGATGAGTATAATGCAGCAGCCTGA
Protein sequence

>Msa0003130

MDSQGQTTSLQRLQNVEKRIVKVLELAGGVMDELASPVGPRKDLVQNHCLEFMQLIKDIQVALRDEIKSACEYRPFEKCDYGPRIANEICFKKVEYVMSQLEAMKQTIDEYNAAA*